Cucsa.189340.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.189340.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Plant invertase/pectin methylesterase inhibitor
Location	scaffold01305 : 40488 .. 40760 (-)
Sequence length	273

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTCGTTTTGAAATTGCGCTAA

mRNA sequence

GACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTCGTTTTGAAATTGCGCTAA

Coding sequence (CDS)

GACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTCGTTTTGAAATTGCGCTAA

Protein sequence

DSWGIDEQQADEKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLLPLVLKLR*

Homology

[Close]

BLAST of Cucsa.189340.1 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 3.1e-06
Identity = 24/84 (28.57%), Postives = 44/84 (52.38%), Query Frame = 1


Query: 5   IDEQQADEKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTN 64
           I E Q D + +     C ++ +  +DEL  S+  L +      D  + +L  W++ A+++
Sbjct: 110 IMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISH 169

Query: 65  EDTCIEGFEGERGKVVNLLPLVLK 89
           E+TC+EGF+G +G     +   LK
Sbjct: 170 EETCLEGFQGTQGNAGETMKKALK 193

[Close]

BLAST of Cucsa.189340.1 vs. TrEMBL
Match: A0A0A0KAX0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 5.6e-23
Identity = 54/63 (85.71%), Postives = 58/63 (92.06%), Query Frame = 1


Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           CV+VFEA IDELHRS GVLRRLSRRNFDAQMGDLTTWV+ ALT+EDTC+EGFEGE GKVV
Sbjct: 103 CVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

Query: 81  NLL 84
            LL
Sbjct: 163 TLL 165

[Close]

BLAST of Cucsa.189340.1 vs. TrEMBL
Match: A0A0A0L1N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G615740 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 6.4e-19
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 1


Query: 23 KVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNL 82
          KVFEA IDELH S+GVLRRLSRRNFD QMG LTTWV+ ALTNED C++GFEG R K+VNL
Sbjct: 27 KVFEAAIDELHMSFGVLRRLSRRNFDTQMGYLTTWVSIALTNEDICVKGFEGGREKIVNL 86

Query: 83 L 84
          L
Sbjct: 87 L 87

[Close]

BLAST of Cucsa.189340.1 vs. TrEMBL
Match: M5VKL3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016389mg PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.7e-16
Identity = 40/63 (63.49%), Postives = 50/63 (79.37%), Query Frame = 1


Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           C++ F+  IDELH+S GVLR LSR  FD QMGDL TW++ ALTN DTC++GFEG+RG+ V
Sbjct: 92  CIECFQNAIDELHKSLGVLRSLSRTTFDTQMGDLNTWLSAALTNGDTCLDGFEGQRGRQV 151

Query: 81  NLL 84
            LL
Sbjct: 152 KLL 154

[Close]

BLAST of Cucsa.189340.1 vs. TrEMBL
Match: A0A0S3S0J0_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G394100 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.4e-15
Identity = 40/71 (56.34%), Postives = 50/71 (70.42%), Query Frame = 1


Query: 13  KEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGF 72
           + K     CV+ F   +DELHRS GVLRRLS+  F  QMGDL TW++ ALT+E+TC+EGF
Sbjct: 95  RNKVALSDCVECFSYALDELHRSLGVLRRLSKSTFSTQMGDLNTWISAALTDEETCLEGF 154

Query: 73  EGERGKVVNLL 84
           EGE  K V +L
Sbjct: 155 EGENEKHVKVL 165

[Close]

BLAST of Cucsa.189340.1 vs. TrEMBL
Match: A0A0L9U9W5_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g284300 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.4e-15
Identity = 40/71 (56.34%), Postives = 50/71 (70.42%), Query Frame = 1


Query: 13  KEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGF 72
           + K     CV+ F   +DELHRS GVLRRLS+  F  QMGDL TW++ ALT+E+TC+EGF
Sbjct: 95  RNKVALSDCVECFSYALDELHRSLGVLRRLSKSTFSTQMGDLNTWISAALTDEETCLEGF 154

Query: 73  EGERGKVVNLL 84
           EGE  K V +L
Sbjct: 155 EGENEKHVKVL 165

[Close]

BLAST of Cucsa.189340.1 vs. TAIR10
Match: AT2G47670.1 (AT2G47670.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 4.9e-10
Identity = 26/62 (41.94%), Postives = 41/62 (66.13%), Query Frame = 1


Query: 12  EKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEG 71
           E E+     C ++F  ++D L++S  VLR L+   F  Q+ DL TW++ ALT++DTC++G
Sbjct: 109 ESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDDTCLDG 168

Query: 72  FE 74
           FE
Sbjct: 169 FE 170

[Close]

BLAST of Cucsa.189340.1 vs. TAIR10
Match: AT3G62820.1 (AT3G62820.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 60.5 bits (145), Expect = 6.4e-10
Identity = 24/62 (38.71%), Postives = 42/62 (67.74%), Query Frame = 1


Query: 12  EKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEG 71
           ++++     C ++ + ++D LH+S  VLR L    F  QM DL TW++++LT++DTC++G
Sbjct: 94  KRDRIALSDCRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLTDKDTCLDG 153

Query: 72  FE 74
           FE
Sbjct: 154 FE 155

[Close]

BLAST of Cucsa.189340.1 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 2.4e-09
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1


Query: 13  KEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGF 72
           KE      CV+    T++EL +S G + +LS  N++  + D+ TWV+ ALT+ +TC +GF
Sbjct: 105 KEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAALTDVNTCTDGF 164

Query: 73  EGE 76
           EGE
Sbjct: 165 EGE 167

[Close]

BLAST of Cucsa.189340.1 vs. TAIR10
Match: AT5G20740.1 (AT5G20740.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.4 bits (137), Expect = 5.4e-09
Identity = 27/68 (39.71%), Postives = 43/68 (63.24%), Query Frame = 1


Query: 12  EKEKQGSPSCVKVFEATIDELHRSYGVLRRL-----SRRNFDAQMGDLTTWVNTALTNED 71
           ++EK     CV++   ++DEL R+ GVL+ L     S + F  QM +  TW + ALT++D
Sbjct: 95  KQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDD 154

Query: 72  TCIEGFEG 75
           TC++GF+G
Sbjct: 155 TCLDGFQG 162

[Close]

BLAST of Cucsa.189340.1 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-07
Identity = 24/84 (28.57%), Postives = 44/84 (52.38%), Query Frame = 1


Query: 5   IDEQQADEKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTN 64
           I E Q D + +     C ++ +  +DEL  S+  L +      D  + +L  W++ A+++
Sbjct: 110 IMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISH 169

Query: 65  EDTCIEGFEGERGKVVNLLPLVLK 89
           E+TC+EGF+G +G     +   LK
Sbjct: 170 EETCLEGFQGTQGNAGETMKKALK 193

[Close]

BLAST of Cucsa.189340.1 vs. NCBI nr
Match: gi|778728057|ref|XP_011659361.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 114.8 bits (286), Expect = 8.1e-23
Identity = 54/63 (85.71%), Postives = 58/63 (92.06%), Query Frame = 1


Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           CV+VFEA IDELHRS GVLRRLSRRNFDAQMGDLTTWV+ ALT+EDTC+EGFEGE GKVV
Sbjct: 103 CVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

Query: 81  NLL 84
            LL
Sbjct: 163 TLL 165

[Close]

BLAST of Cucsa.189340.1 vs. NCBI nr
Match: gi|659101359|ref|XP_008451567.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 114.8 bits (286), Expect = 8.1e-23
Identity = 53/63 (84.13%), Postives = 60/63 (95.24%), Query Frame = 1


Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           CV+VF+A IDELHRS GVLRRLSRRNFD+QMGDLTTWV+ ALT+EDTC+EGFEGERGKVV
Sbjct: 103 CVEVFDAAIDELHRSLGVLRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVV 162

Query: 81  NLL 84
           +LL
Sbjct: 163 SLL 165

[Close]

BLAST of Cucsa.189340.1 vs. NCBI nr
Match: gi|700199796|gb|KGN54954.1| (hypothetical protein Csa_4G615740 [Cucumis sativus])

HSP 1 Score: 101.3 bits (251), Expect = 9.2e-19
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 1


Query: 23 KVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNL 82
          KVFEA IDELH S+GVLRRLSRRNFD QMG LTTWV+ ALTNED C++GFEG R K+VNL
Sbjct: 27 KVFEAAIDELHMSFGVLRRLSRRNFDTQMGYLTTWVSIALTNEDICVKGFEGGREKIVNL 86

Query: 83 L 84
          L
Sbjct: 87 L 87

[Close]

BLAST of Cucsa.189340.1 vs. NCBI nr
Match: gi|595792857|ref|XP_007200177.1| (hypothetical protein PRUPE_ppa016389mg [Prunus persica])

HSP 1 Score: 91.3 bits (225), Expect = 9.5e-16
Identity = 40/63 (63.49%), Postives = 50/63 (79.37%), Query Frame = 1


Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           C++ F+  IDELH+S GVLR LSR  FD QMGDL TW++ ALTN DTC++GFEG+RG+ V
Sbjct: 92  CIECFQNAIDELHKSLGVLRSLSRTTFDTQMGDLNTWLSAALTNGDTCLDGFEGQRGRQV 151

Query: 81  NLL 84
            LL
Sbjct: 152 KLL 154

[Close]

BLAST of Cucsa.189340.1 vs. NCBI nr
Match: gi|645258878|ref|XP_008235094.1| (PREDICTED: 21 kDa protein-like [Prunus mume])

HSP 1 Score: 90.1 bits (222), Expect = 2.1e-15
Identity = 39/63 (61.90%), Postives = 50/63 (79.37%), Query Frame = 1


Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           C++ F+ +IDELH+S GVLR LSR  FD QMGDL TW++  LTN DTC++GFEG+RG+ V
Sbjct: 106 CIECFQNSIDELHKSLGVLRSLSRTTFDTQMGDLNTWLSATLTNGDTCLDGFEGQRGRQV 165

Query: 81  NLL 84
            LL
Sbjct: 166 KLL 168

The following BLAST results are available for this feature:

BLAST of Cucsa.189340.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME21_ARATH	3.1e-06	28.57	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=P...	[more]

BLAST of Cucsa.189340.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAX0_CUCSA	5.6e-23	85.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1	[more]
A0A0A0L1N4_CUCSA	6.4e-19	78.69	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G615740 PE=4 SV=1	[more]
M5VKL3_PRUPE	6.7e-16	63.49	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016389mg PE=4 SV=1	[more]
A0A0S3S0J0_PHAAN	7.4e-15	56.34	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G394100 PE=...	[more]
A0A0L9U9W5_PHAAN	7.4e-15	56.34	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g284300 PE=4 SV=1	[more]

BLAST of Cucsa.189340.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G47670.1	4.9e-10	41.94	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G62820.1	6.4e-10	38.71	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	2.4e-09	41.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G20740.1	5.4e-09	39.71	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G05610.1	1.7e-07	28.57	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of Cucsa.189340.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778728057\|ref\|XP_011659361.1\|	8.1e-23	85.71	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|659101359\|ref\|XP_008451567.1\|	8.1e-23	84.13	PREDICTED: 21 kDa protein [Cucumis melo]	[more]
gi\|700199796\|gb\|KGN54954.1\|	9.2e-19	78.69	hypothetical protein Csa_4G615740 [Cucumis sativus]	[more]
gi\|595792857\|ref\|XP_007200177.1\|	9.5e-16	63.49	hypothetical protein PRUPE_ppa016389mg [Prunus persica]	[more]
gi\|645258878\|ref\|XP_008235094.1\|	2.1e-15	61.90	PREDICTED: 21 kDa protein-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.189340	Cucsa.189340	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.189340.1	Cucsa.189340.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.189340.1.CDS.1	Cucsa.189340.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 8..80 score: 1.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 15..80 score: 1.
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 8..81 score: 1.5
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 9..84 score: 8.5
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 21..83 score: 6.2
None	No IPR available	PANTHER	PTHR31080:SF16	SUBFAMILY NOT NAMED	coord: 21..83 score: 6.2