Cucsa.189340 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.189340
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor
Locationscaffold01305 : 40488 .. 40760 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTCGTTTTGAAATTGCGCTAA

mRNA sequence

GACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTCGTTTTGAAATTGCGCTAA

Coding sequence (CDS)

GACTCCTGGGGGATTGACGAACAACAAGCGGATGAAAAGGAGAAACAAGGCAGTCCAAGCTGTGTGAAGGTGTTTGAAGCCACAATTGATGAGCTTCACAGGTCGTATGGGGTTCTTAGAAGATTGAGTAGGAGGAATTTTGACGCACAAATGGGGGATCTAACGACGTGGGTCAACACTGCACTGACGAATGAAGATACTTGCATTGAAGGATTTGAAGGTGAAAGGGGAAAAGTAGTGAATTTGTTGCCTCTCGTTTTGAAATTGCGCTAA

Protein sequence

DSWGIDEQQADEKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNLLPLVLKLR*
Homology
[Close]
BLAST of Cucsa.189340 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 3.1e-06
Identity = 24/84 (28.57%), Postives = 44/84 (52.38%), Query Frame = 1 

Query: 5   IDEQQADEKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTN 64
           I E Q D + +     C ++ +  +DEL  S+  L +      D  + +L  W++ A+++
Sbjct: 110 IMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISH 169

Query: 65  EDTCIEGFEGERGKVVNLLPLVLK 89
           E+TC+EGF+G +G     +   LK
Sbjct: 170 EETCLEGFQGTQGNAGETMKKALK 193

[Close]
BLAST of Cucsa.189340 vs. TrEMBL
Match: A0A0A0KAX0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 5.6e-23
Identity = 54/63 (85.71%), Postives = 58/63 (92.06%), Query Frame = 1 

Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           CV+VFEA IDELHRS GVLRRLSRRNFDAQMGDLTTWV+ ALT+EDTC+EGFEGE GKVV
Sbjct: 103 CVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

Query: 81  NLL 84
            LL
Sbjct: 163 TLL 165

[Close]
BLAST of Cucsa.189340 vs. TrEMBL
Match: A0A0A0L1N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G615740 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 6.4e-19
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 1 

Query: 23 KVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNL 82
          KVFEA IDELH S+GVLRRLSRRNFD QMG LTTWV+ ALTNED C++GFEG R K+VNL
Sbjct: 27 KVFEAAIDELHMSFGVLRRLSRRNFDTQMGYLTTWVSIALTNEDICVKGFEGGREKIVNL 86

Query: 83 L 84
          L
Sbjct: 87 L 87

[Close]
BLAST of Cucsa.189340 vs. TrEMBL
Match: M5VKL3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016389mg PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 6.7e-16
Identity = 40/63 (63.49%), Postives = 50/63 (79.37%), Query Frame = 1 

Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           C++ F+  IDELH+S GVLR LSR  FD QMGDL TW++ ALTN DTC++GFEG+RG+ V
Sbjct: 92  CIECFQNAIDELHKSLGVLRSLSRTTFDTQMGDLNTWLSAALTNGDTCLDGFEGQRGRQV 151

Query: 81  NLL 84
            LL
Sbjct: 152 KLL 154

[Close]
BLAST of Cucsa.189340 vs. TrEMBL
Match: A0A0S3S0J0_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G394100 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.4e-15
Identity = 40/71 (56.34%), Postives = 50/71 (70.42%), Query Frame = 1 

Query: 13  KEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGF 72
           + K     CV+ F   +DELHRS GVLRRLS+  F  QMGDL TW++ ALT+E+TC+EGF
Sbjct: 95  RNKVALSDCVECFSYALDELHRSLGVLRRLSKSTFSTQMGDLNTWISAALTDEETCLEGF 154

Query: 73  EGERGKVVNLL 84
           EGE  K V +L
Sbjct: 155 EGENEKHVKVL 165

[Close]
BLAST of Cucsa.189340 vs. TrEMBL
Match: A0A0L9U9W5_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g284300 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.4e-15
Identity = 40/71 (56.34%), Postives = 50/71 (70.42%), Query Frame = 1 

Query: 13  KEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGF 72
           + K     CV+ F   +DELHRS GVLRRLS+  F  QMGDL TW++ ALT+E+TC+EGF
Sbjct: 95  RNKVALSDCVECFSYALDELHRSLGVLRRLSKSTFSTQMGDLNTWISAALTDEETCLEGF 154

Query: 73  EGERGKVVNLL 84
           EGE  K V +L
Sbjct: 155 EGENEKHVKVL 165

[Close]
BLAST of Cucsa.189340 vs. TAIR10
Match: AT2G47670.1 (AT2G47670.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 4.9e-10
Identity = 26/62 (41.94%), Postives = 41/62 (66.13%), Query Frame = 1 

Query: 12  EKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEG 71
           E E+     C ++F  ++D L++S  VLR L+   F  Q+ DL TW++ ALT++DTC++G
Sbjct: 109 ESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDDTCLDG 168

Query: 72  FE 74
           FE
Sbjct: 169 FE 170

[Close]
BLAST of Cucsa.189340 vs. TAIR10
Match: AT3G62820.1 (AT3G62820.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 60.5 bits (145), Expect = 6.4e-10
Identity = 24/62 (38.71%), Postives = 42/62 (67.74%), Query Frame = 1 

Query: 12  EKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEG 71
           ++++     C ++ + ++D LH+S  VLR L    F  QM DL TW++++LT++DTC++G
Sbjct: 94  KRDRIALSDCRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLTDKDTCLDG 153

Query: 72  FE 74
           FE
Sbjct: 154 FE 155

[Close]
BLAST of Cucsa.189340 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 2.4e-09
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1 

Query: 13  KEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGF 72
           KE      CV+    T++EL +S G + +LS  N++  + D+ TWV+ ALT+ +TC +GF
Sbjct: 105 KEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAALTDVNTCTDGF 164

Query: 73  EGE 76
           EGE
Sbjct: 165 EGE 167

[Close]
BLAST of Cucsa.189340 vs. TAIR10
Match: AT5G20740.1 (AT5G20740.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 57.4 bits (137), Expect = 5.4e-09
Identity = 27/68 (39.71%), Postives = 43/68 (63.24%), Query Frame = 1 

Query: 12  EKEKQGSPSCVKVFEATIDELHRSYGVLRRL-----SRRNFDAQMGDLTTWVNTALTNED 71
           ++EK     CV++   ++DEL R+ GVL+ L     S + F  QM +  TW + ALT++D
Sbjct: 95  KQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDD 154

Query: 72  TCIEGFEG 75
           TC++GF+G
Sbjct: 155 TCLDGFQG 162

[Close]
BLAST of Cucsa.189340 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 52.4 bits (124), Expect = 1.7e-07
Identity = 24/84 (28.57%), Postives = 44/84 (52.38%), Query Frame = 1 

Query: 5   IDEQQADEKEKQGSPSCVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTN 64
           I E Q D + +     C ++ +  +DEL  S+  L +      D  + +L  W++ A+++
Sbjct: 110 IMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISH 169

Query: 65  EDTCIEGFEGERGKVVNLLPLVLK 89
           E+TC+EGF+G +G     +   LK
Sbjct: 170 EETCLEGFQGTQGNAGETMKKALK 193

[Close]
BLAST of Cucsa.189340 vs. NCBI nr
Match: gi|778728057|ref|XP_011659361.1| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 114.8 bits (286), Expect = 8.1e-23
Identity = 54/63 (85.71%), Postives = 58/63 (92.06%), Query Frame = 1 

Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           CV+VFEA IDELHRS GVLRRLSRRNFDAQMGDLTTWV+ ALT+EDTC+EGFEGE GKVV
Sbjct: 103 CVEVFEAAIDELHRSLGVLRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

Query: 81  NLL 84
            LL
Sbjct: 163 TLL 165

[Close]
BLAST of Cucsa.189340 vs. NCBI nr
Match: gi|659101359|ref|XP_008451567.1| (PREDICTED: 21 kDa protein [Cucumis melo])

HSP 1 Score: 114.8 bits (286), Expect = 8.1e-23
Identity = 53/63 (84.13%), Postives = 60/63 (95.24%), Query Frame = 1 

Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           CV+VF+A IDELHRS GVLRRLSRRNFD+QMGDLTTWV+ ALT+EDTC+EGFEGERGKVV
Sbjct: 103 CVEVFDAAIDELHRSLGVLRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVV 162

Query: 81  NLL 84
           +LL
Sbjct: 163 SLL 165

[Close]
BLAST of Cucsa.189340 vs. NCBI nr
Match: gi|700199796|gb|KGN54954.1| (hypothetical protein Csa_4G615740 [Cucumis sativus])

HSP 1 Score: 101.3 bits (251), Expect = 9.2e-19
Identity = 48/61 (78.69%), Postives = 53/61 (86.89%), Query Frame = 1 

Query: 23 KVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVVNL 82
          KVFEA IDELH S+GVLRRLSRRNFD QMG LTTWV+ ALTNED C++GFEG R K+VNL
Sbjct: 27 KVFEAAIDELHMSFGVLRRLSRRNFDTQMGYLTTWVSIALTNEDICVKGFEGGREKIVNL 86

Query: 83 L 84
          L
Sbjct: 87 L 87

[Close]
BLAST of Cucsa.189340 vs. NCBI nr
Match: gi|595792857|ref|XP_007200177.1| (hypothetical protein PRUPE_ppa016389mg [Prunus persica])

HSP 1 Score: 91.3 bits (225), Expect = 9.5e-16
Identity = 40/63 (63.49%), Postives = 50/63 (79.37%), Query Frame = 1 

Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           C++ F+  IDELH+S GVLR LSR  FD QMGDL TW++ ALTN DTC++GFEG+RG+ V
Sbjct: 92  CIECFQNAIDELHKSLGVLRSLSRTTFDTQMGDLNTWLSAALTNGDTCLDGFEGQRGRQV 151

Query: 81  NLL 84
            LL
Sbjct: 152 KLL 154

[Close]
BLAST of Cucsa.189340 vs. NCBI nr
Match: gi|645258878|ref|XP_008235094.1| (PREDICTED: 21 kDa protein-like [Prunus mume])

HSP 1 Score: 90.1 bits (222), Expect = 2.1e-15
Identity = 39/63 (61.90%), Postives = 50/63 (79.37%), Query Frame = 1 

Query: 21  CVKVFEATIDELHRSYGVLRRLSRRNFDAQMGDLTTWVNTALTNEDTCIEGFEGERGKVV 80
           C++ F+ +IDELH+S GVLR LSR  FD QMGDL TW++  LTN DTC++GFEG+RG+ V
Sbjct: 106 CIECFQNSIDELHKSLGVLRSLSRTTFDTQMGDLNTWLSATLTNGDTCLDGFEGQRGRQV 165

Query: 81  NLL 84
            LL
Sbjct: 166 KLL 168

The following BLAST results are available for this feature:
BLAST of Cucsa.189340 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PME21_ARATH3.1e-0628.57Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=P... [more]
BLAST of Cucsa.189340 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KAX0_CUCSA5.6e-2385.71Uncharacterized protein OS=Cucumis sativus GN=Csa_7G397050 PE=4 SV=1[more]
A0A0A0L1N4_CUCSA6.4e-1978.69Uncharacterized protein OS=Cucumis sativus GN=Csa_4G615740 PE=4 SV=1[more]
M5VKL3_PRUPE6.7e-1663.49Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016389mg PE=4 SV=1[more]
A0A0S3S0J0_PHAAN7.4e-1556.34Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G394100 PE=... [more]
A0A0L9U9W5_PHAAN7.4e-1556.34Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan03g284300 PE=4 SV=1[more]
BLAST of Cucsa.189340 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G47670.14.9e-1041.94 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G62820.16.4e-1038.71 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62360.12.4e-0941.27 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G20740.15.4e-0939.71 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT3G05610.11.7e-0728.57 Plant invertase/pectin methylesterase inhibitor superfamily[more]
BLAST of Cucsa.189340 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778728057|ref|XP_011659361.1|8.1e-2385.71PREDICTED: 21 kDa protein [Cucumis sativus][more]
gi|659101359|ref|XP_008451567.1|8.1e-2384.13PREDICTED: 21 kDa protein [Cucumis melo][more]
gi|700199796|gb|KGN54954.1|9.2e-1978.69hypothetical protein Csa_4G615740 [Cucumis sativus][more]
gi|595792857|ref|XP_007200177.1|9.5e-1663.49hypothetical protein PRUPE_ppa016389mg [Prunus persica][more]
gi|645258878|ref|XP_008235094.1|2.1e-1561.90PREDICTED: 21 kDa protein-like [Prunus mume][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.189340.1Cucsa.189340.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 8..80
score: 1.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 15..80
score: 1.
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 8..81
score: 1.5
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 9..84
score: 8.5
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 21..83
score: 6.2
NoneNo IPR availablePANTHERPTHR31080:SF16SUBFAMILY NOT NAMEDcoord: 21..83
score: 6.2

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.189340Melon (DHL92) v3.6.1cgymedB340
Cucsa.189340Silver-seed gourdcarcgyB0782
Cucsa.189340Cucumber (Chinese Long) v3cgycucB304
Cucsa.189340Watermelon (97103) v2cgywmbB352
Cucsa.189340Watermelon (97103) v2cgywmbB353
Cucsa.189340Wax gourdcgywgoB394
Cucsa.189340Cucurbita maxima (Rimu)cgycmaB0552
Cucsa.189340Cucurbita moschata (Rifu)cgycmoB0553
Cucsa.189340Cucurbita moschata (Rifu)cgycmoB0554
Cucsa.189340Wild cucumber (PI 183967)cgycpiB301
Cucsa.189340Cucumber (Chinese Long) v2cgycuB292
Cucsa.189340Melon (DHL92) v3.5.1cgymeB345
Cucsa.189340Watermelon (Charleston Gray)cgywcgB346
Cucsa.189340Watermelon (Charleston Gray)cgywcgB347
Cucsa.189340Watermelon (97103) v1cgywmB368
Cucsa.189340Watermelon (97103) v1cgywmB369
Cucsa.189340Cucurbita pepo (Zucchini)cgycpeB0533
Cucsa.189340Cucurbita pepo (Zucchini)cgycpeB0534
Cucsa.189340Bottle gourd (USVL1VR-Ls)cgylsiB324