Cucsa.153660.2 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.153660.2 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 1.9e-206
Identity = 368/680 (54.12%), Postives = 461/680 (67.79%), Query Frame = 1

Query: 1   MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKR 60
           + E   FERIYDYD YND+GD D   E  RPV+GG  HPYPRRC+TGR  C+TDP SE+R
Sbjct: 231 VGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQR 290

Query: 61  SKERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLL--GKDSFSDLPHIESMFRE 120
               FYVPRDEEFS  K   F         PS  P  + +L   ++ F     I+++F E
Sbjct: 291 YGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEE 350

Query: 121 GIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTE 180
           GI+ P     L          P  P +   +       +L +  P    RDR+SWL D E
Sbjct: 351 GIQLPKDAGLL----------PLLPRI-IKALGEAQDDILQFDAPVLINRDRFSWLRDDE 410

Query: 181 FARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELK 240
           FARQTLAGLNPYSIQLV   PL+S+LDP  YG   S      V+  +  ++ V+EA++ K
Sbjct: 411 FARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNK 470

Query: 241 RLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWK 300
           RLFVLDYHD L+PYVNKVR++N TTLY SRTLFFL  D TL P+ IELT PP    PQWK
Sbjct: 471 RLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWK 530

Query: 301 QIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPI 360
           Q+FTPG +AT  WLW +AK H ++HD+  HQL+ HWLR H C EPY IA NRQLS +HPI
Sbjct: 531 QVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPI 590

Query: 361 YRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPE 420
           YRLLHPHFRY M INA AR+SL+N GGIIE+ F    Y++ELSS+VY   WRFD++ LP 
Sbjct: 591 YRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPA 650

Query: 421 DLIRRGMAER-KKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAV 480
           DLI+RG+AE  K  ++G   + L I DYPFANDGL+LW+A+ EWVT+YV HYY D+E  +
Sbjct: 651 DLIKRGLAEEDKTAEHG---VRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEE-LI 710

Query: 481 INDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYA 540
            +D+ELQ WW+E++  GH DKK+   WP LKT++DLI + +TIAWV SGHHA+VNF QY 
Sbjct: 711 TSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYG 770

Query: 541 YAGYTPNRPSIARTNILTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAH 600
           Y GY PNRP+  R  + TED   +  +EF + PE  LL+ +PS  QA++V  T+ LLS H
Sbjct: 771 YGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTH 830

Query: 601 SPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYD 660
           SPDEEYIG++ E +WA EP I+ AF+RF+  L+ LE  ID+ N N  LKNR GAGVV Y+
Sbjct: 831 SPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYE 890

Query: 661 VLKPTSTYGITGRGVPYSVS 666
           +LKPTS +G+TG GVPYS+S
Sbjct: 891 LLKPTSEHGVTGMGVPYSIS 895

BLAST of Cucsa.153660.2 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.2e-203
Identity = 374/683 (54.76%), Postives = 472/683 (69.11%), Query Frame = 1

Query: 1   MNERKAFERIYDYDFYNDLGDCDGPAE-WKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEK 60
           + ERK FERIYDYD YNDLG+ D   +  KRPVLGG + PYPRRC+TGR   K DP+SE 
Sbjct: 229 IGERKVFERIYDYDVYNDLGEADSNNDDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSET 288

Query: 61  RSKERFYVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKDS--FSDLPHIESMFR 120
           RS    YVPRDE FSEVK   F  +          P  + ++   +  F   P I+S+F 
Sbjct: 289 RSTF-VYVPRDEAFSEVKSVAFSGNTVYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFN 348

Query: 121 EGIKAPHAPHKLL-TFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSD 180
            G+  P    K    FN    V P      S++   V    L +  P+  +RD++SW  D
Sbjct: 349 VGVDLPGLGDKKSGLFN----VVPRLIKAISDTRKDV----LLFESPQLVQRDKFSWFRD 408

Query: 181 TEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIE 240
            EFARQTLAGLNPYSI+LVT  PL S+LDP  YGP ES      +++ IG  + V +A++
Sbjct: 409 VEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQAVQ 468

Query: 241 LKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQ 300
            K+LF+LDYHD L+PYVNKV ++ G+ LYGSRT+FFL    TL PL IELTRPPVD  PQ
Sbjct: 469 QKKLFILDYHDLLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDKPQ 528

Query: 301 WKQIFTPGI-EATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTV 360
           WK++++P    AT  WLW++AKAHVL+HDS  HQLV HWLR HCC EPY IA+NRQLS +
Sbjct: 529 WKEVYSPNDWNATGAWLWKLAKAHVLSHDSGYHQLVSHWLRTHCCTEPYIIASNRQLSAM 588

Query: 361 HPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQA 420
           HPIYRLLHPHFRY M INA ARE+LINA G+IES+F    Y++ELSS  Y  +WRFD++A
Sbjct: 589 HPIYRLLHPHFRYTMEINALAREALINANGVIESSFFPGKYAIELSSIAYGAEWRFDQEA 648

Query: 421 LPEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDE 480
           LP++LI RG+A E     +G   L+LAI+DYPFANDGL+LW+ L +WVT YVNHYY    
Sbjct: 649 LPQNLISRGLAVEDPNEPHG---LKLAIEDYPFANDGLVLWDILKQWVTNYVNHYY-PQT 708

Query: 481 NAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFI 540
           N + +DKELQAWW+EI+  GH DK++   WP LKT NDLI I +TI WV SGHHA+VNF 
Sbjct: 709 NLIESDKELQAWWSEIKNVGHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFG 768

Query: 541 QYAYAGYTPNRPSIARTNILTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILL 600
           QY+YAGY PNRP++AR+ + TED   +  E F++ PE  LL+ FPS  QA+ V   + +L
Sbjct: 769 QYSYAGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVL 828

Query: 601 SAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVV 660
           S HSPDEEYIG++IEP WA +P I+ AF+ F   LK+LE  ID  N ++KL NR+GAGV+
Sbjct: 829 SNHSPDEEYIGEKIEPYWAEDPVINAAFEVFSGKLKELEGIIDARNNDSKLSNRNGAGVM 888

Query: 661 PYDVLKPTSTYGITGRGVPYSVS 666
           PY++LKP S  G+TG+GVPYS+S
Sbjct: 889 PYELLKPYSEPGVTGKGVPYSIS 898

BLAST of Cucsa.153660.2 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.8e-197
Identity = 353/677 (52.14%), Postives = 456/677 (67.36%), Query Frame = 1

Query: 2   NERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRS 61
           +ERK  ER+YDYD YNDLGD D   + KRPVLG  +HPYPRRCRTGR     DP +E RS
Sbjct: 229 SERKFHERVYDYDTYNDLGDPDKNIDHKRPVLGTKEHPYPRRCRTGRPKTLYDPETETRS 288

Query: 62  KERFYVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKD-SFSDLPHIESMFREGI 121
               YVPRDE+FS+VK   F ++          P    LL     FS  P I++++ +GI
Sbjct: 289 SP-VYVPRDEQFSDVKGRTFSATTLRSGLHAILPAVAPLLNNSHGFSHFPAIDALYSDGI 348

Query: 122 KAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFA 181
             P   H   +FN+   V P    +  ++   V    L +  PE   RDR+SW  D EFA
Sbjct: 349 PLPVDGHGGNSFNVINDVIPRVVQMIEDTTEHV----LRFEVPEMLERDRFSWFRDEEFA 408

Query: 182 RQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRL 241
           RQTLAGLNP  I+ +T  P++S+LDP  YGP ESA +   +++++   + V EA+E KRL
Sbjct: 409 RQTLAGLNPICIRRLTEFPIVSKLDPAVYGPAESALSKEILEKMMNGRMTVEEAMEKKRL 468

Query: 242 FVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQI 301
           F+LDYHD  +PYV++VR++  TTLYGSRT+FFL  + TL+PL IELTRP     PQWK+ 
Sbjct: 469 FLLDYHDVFLPYVHRVRELPDTTLYGSRTVFFLSDEGTLMPLAIELTRPQSPTKPQWKRA 528

Query: 302 FTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYR 361
           FT G +AT+ WLW++AKAHVL HD+  HQLV HWLR H C+EPY IATNRQLS +HP+YR
Sbjct: 529 FTHGSDATESWLWKLAKAHVLTHDTGYHQLVSHWLRTHACVEPYIIATNRQLSRMHPVYR 588

Query: 362 LLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDL 421
           LLHPHFRY M INA ARE+LINA GIIE  F    YS+ELSS  Y   W+F+ +ALPEDL
Sbjct: 589 LLHPHFRYTMEINALAREALINADGIIEEAFLAGKYSIELSSVAYGAAWQFNTEALPEDL 648

Query: 422 IRRGMAERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVIND 481
           I RG+A R+  D G   LELAIKDYP+A+DGLL+W ++ +W ++YV+ YY  D + V  D
Sbjct: 649 INRGLAVRR--DDGE--LELAIKDYPYADDGLLIWGSIKQWASDYVDFYYKSDGD-VAGD 708

Query: 482 KELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAG 541
           +EL+AWW E++ KGH DKK+   WP   T+ +L++I + I WV SGHHA+VNF QY YAG
Sbjct: 709 EELRAWWEEVRTKGHADKKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAVNFGQYHYAG 768

Query: 542 YTPNRPSIARTNI-LTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPD 601
           Y PNRP++ R NI + E+     ++F+  PE  LLQ  PS  QA  V  T+ +LS+HSPD
Sbjct: 769 YFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPD 828

Query: 602 EEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLK 661
           EEY+G+  EPAW  EP +  AF++F   LK+ E  ID  N N + KNR GAG+VPY++LK
Sbjct: 829 EEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEAEGTIDMRNNNPENKNRCGAGIVPYELLK 888

Query: 662 PTSTYGITGRGVPYSVS 666
           P S  G+TGRG+P S+S
Sbjct: 889 PFSEPGVTGRGIPNSIS 895

BLAST of Cucsa.153660.2 vs. Swiss-Prot
Match: LOX21_HORVU (Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 7.9e-184
Identity = 340/698 (48.71%), Postives = 443/698 (63.47%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAE-WKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRS 62
           ERK  +RIYDYD YNDLG+ D       RPVLGG +HPYPRRCRTGR   K DP SE+RS
Sbjct: 248 ERKEHDRIYDYDVYNDLGNPDDDNNPTTRPVLGGKEHPYPRRCRTGRPRSKKDPFSEERS 307

Query: 63  -KERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGK---------------DSFSDLPHIES 122
            KE  YVPRDE F+E K   F     D K  + +                SF  L  I+ 
Sbjct: 308 HKEHIYVPRDEAFTERKMGAF-----DTKKFMSQLHALTTGLKTAKHKSQSFPSLSAIDQ 367

Query: 123 MFREGIK-APHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSL---LHYPPPESYRRDR 182
           ++ +  +  P  P       +  ++  +   L     ++    +     +  PE + RD+
Sbjct: 368 LYDDNFRNQPVQPEGGKLRFVIDLLETELLHLFKLEGAAFLEGIRRVFKFETPEIHDRDK 427

Query: 183 YSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIE 242
           ++W  D EFARQT+AG+NP SIQLVT  P+ S LD  TYGP +S      V+E I   + 
Sbjct: 428 FAWFRDEEFARQTIAGMNPMSIQLVTEFPIKSNLDEATYGPADSLITKEVVEEQIRRVMT 487

Query: 243 VNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPP 302
            +EA++ K+LF+LDYHD L+PYV+KVRK++GTTLYGSR LFFL +D TL P+ IELTRP 
Sbjct: 488 ADEAVQNKKLFMLDYHDLLLPYVHKVRKLDGTTLYGSRALFFLTADGTLRPIAIELTRPK 547

Query: 303 VDGYPQWKQIFTPGIEA--TDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIAT 362
               PQW+Q+FTPG +   T  WLW++AKAH+LAHD+ +HQLV HWLR H C EPY IA 
Sbjct: 548 SKKKPQWRQVFTPGCDGSVTGSWLWQLAKAHILAHDAGVHQLVSHWLRTHACTEPYIIAA 607

Query: 363 NRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQ 422
           NRQLS +HP+YRLLHPHFR+ M INA AR  LINAGGIIE +F    YS+ELSS  Y  Q
Sbjct: 608 NRQLSQMHPVYRLLHPHFRFTMEINAQARAMLINAGGIIEGSFVPGEYSLELSSVAYDQQ 667

Query: 423 WRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNH 482
           WRFD +ALPEDLIRRGMA R         LELAI+DYP+ANDGLL+W+A+ +W   YV H
Sbjct: 668 WRFDMEALPEDLIRRGMAVRNP----NGELELAIEDYPYANDGLLVWDAIKQWALTYVQH 727

Query: 483 YYGDDENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHH 542
           YY    + +++D+ELQAWW E++ KGH DK++   WP L +  +L +  +TI WV SGHH
Sbjct: 728 YYPCAAD-IVDDEELQAWWTEVRTKGHADKQDEPWWPELDSHENLAQTLATIMWVTSGHH 787

Query: 543 ASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTT 602
           A+VNF QY  AGY PNRP++AR N+ TE       +F++ PE  LL  FPS  Q+++V  
Sbjct: 788 AAVNFGQYPMAGYIPNRPTMARRNMPTEIGGDDMRDFVEAPEKVLLDTFPSQYQSAIVLA 847

Query: 603 TMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRH 662
            + LLS HS DEEY+G   EPAW  +  I++AF+ F+ S + + +Q+D+ N +   KNRH
Sbjct: 848 ILDLLSTHSSDEEYMGTHEEPAWTKDGVINQAFEEFKESTRKIVEQVDEWNNDPDRKNRH 907

Query: 663 GAGVVPYDVLK----------PTSTYGITGRGVPYSVS 666
           GAG+VPY +L+          PT    +   G+P S+S
Sbjct: 908 GAGMVPYVLLRPSDGDPTDGDPTDEKMVMEMGIPNSIS 935

BLAST of Cucsa.153660.2 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 643.7 bits (1659), Expect = 2.3e-183
Identity = 340/683 (49.78%), Postives = 444/683 (65.01%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDH-PYPRRCRTGRLHCKTDPVSEKRS 62
           ER+A +R+YDYD YNDLG+ D   +  RPVLGG+   PYPRRCRTGR   K DP SE R 
Sbjct: 253 EREADDRVYDYDVYNDLGNPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETR- 312

Query: 63  KERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLLGKDSFSDLPH-----IESMF 122
           K   YVPRDEEFS  K+ YF          ++ P  + LL      +LP      I+ +F
Sbjct: 313 KGNVYVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLF 372

Query: 123 REGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSD 182
            +G++ P      L F  S +     P L  +   +    +L +  P + ++D+++WL D
Sbjct: 373 EDGVELPGVDK--LNFLESVV-----PRLLEHLRDTPAEKILRFETPANIQKDKFAWLRD 432

Query: 183 TEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIE 242
            EFAR+TLAG+NPY+I+LV   PL S+LDP  YGP ESA     ++E +   + V EAI 
Sbjct: 433 EEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAIS 492

Query: 243 LKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQ 302
            KRLF+LD+HD  +PYV+K+R ++ TT+YGSRT+FFL  D TL  L IELTRP     PQ
Sbjct: 493 QKRLFMLDFHDLFLPYVHKIRSLDHTTMYGSRTVFFLTDDGTLQLLAIELTRPASPSQPQ 552

Query: 303 WKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVH 362
           W+Q+FTP  +AT  WLWR+AKAHV AHD+  H+L+ HWLR HC +EPY IA NRQLS +H
Sbjct: 553 WRQVFTPSTDATMSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMH 612

Query: 363 PIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQAL 422
           PIY+LL PHFRY MRINA AR +LI+AGGIIE +FS   YS+ELSS  Y   WRFD +AL
Sbjct: 613 PIYQLLRPHFRYTMRINARARSALISAGGIIERSFSPQKYSMELSSVAYDKLWRFDTEAL 672

Query: 423 PEDLIRRGMAER-KKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDEN 482
           P DL+RRGMAE     ++G   L+LAI+DYPFANDGLL+W+A+  WV  YV  +Y  D +
Sbjct: 673 PADLVRRGMAEEDPTAEHG---LKLAIEDYPFANDGLLIWDAIKTWVQAYVARFY-PDAD 732

Query: 483 AVINDKELQAWWNEIQEKGHPDKKEG--WPTLKTRNDLIKIASTIAWVGSGHHASVNFIQ 542
           +V  D+ELQA+W E++ KGH DKK+   WP L +   L    +TI WV + HHA+VNF Q
Sbjct: 733 SVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQ 792

Query: 543 YAYAGYTPNRPSIARTNILTED--YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILL 602
           Y + GY PNRPSIART +  E+       E F+D P+  L + FPS  QA+VV   + +L
Sbjct: 793 YDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVL 852

Query: 603 SAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVV 662
           S+HS DEEY+G E    W  + ++  A+  F A LK++E  ID  NK+ KLKNR GAG++
Sbjct: 853 SSHSTDEEYLGGEQTRPWNSDAAVQAAYDGFAARLKEIEGVIDGRNKDRKLKNRCGAGIL 912

Query: 663 PYDVLKPTSTYGITGRGVPYSVS 666
           PY ++KP S  G+TG G+P S S
Sbjct: 913 PYQLMKPFSDSGVTGMGIPNSTS 923

BLAST of Cucsa.153660.2 vs. TrEMBL
Match: S4UKZ5_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 1.6e-215
Identity = 395/681 (58.00%), Postives = 479/681 (70.34%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           ERK+FERIYDYD YNDLGD D     +RPVLGG + PYPRRCRTGRL C T  + EKRS+
Sbjct: 273 ERKSFERIYDYDVYNDLGDPDKNLRLERPVLGGKEFPYPRRCRTGRLPCDTGSLYEKRSR 332

Query: 63  ERFYVPRDEEFSEVKQHYF-------------PSSEPDNKDL-LGKDSFSDLPHIESMFR 122
           + +YVPRDE FSEVKQ  F             PS E    D  LG   F     I+S+F 
Sbjct: 333 KHWYVPRDEAFSEVKQLTFSAKTLYSVMHALVPSLEIAIADTDLG---FKYFTAIDSLFH 392

Query: 123 EGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDT 182
           EGI+ P        F    ++    P L    +S     +L + PPE+  RD++ W  D 
Sbjct: 393 EGIQLP-------PFKEQGVLKALLPRLVKVMSSG--DDVLRFVPPETMNRDKFFWFRDD 452

Query: 183 EFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIEL 242
           EFARQTLAGLNPYSI+LVT  PL SELDP+ YGP ESA     +++ I     + EAIE 
Sbjct: 453 EFARQTLAGLNPYSIKLVTEWPLKSELDPEIYGPPESAITKEIIEQEIRGFATITEAIEE 512

Query: 243 KRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQW 302
           K+LF+LDYHD  +PYV+KVRK+ GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQW
Sbjct: 513 KKLFILDYHDLFLPYVSKVRKLEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQW 572

Query: 303 KQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHP 362
           KQ+F P   ATD+WLWR+AKAHVLAHDS  HQLV HWLR HC  EPY IATNRQLS +HP
Sbjct: 573 KQVFQPAWNATDVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHP 632

Query: 363 IYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALP 422
           IYRLLHPHFRY M IN+ ARESLINAGGIIE++FS   YS+EL S VY  +WRFD++ALP
Sbjct: 633 IYRLLHPHFRYTMEINSLARESLINAGGIIETSFSPKKYSLELCSVVYGKEWRFDQEALP 692

Query: 423 EDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENA 482
            DLIRRGMA E     +G   L L I+DYPFANDGLLLW+A+ +WVT+YVNHYY  D + 
Sbjct: 693 ADLIRRGMAVEDPTAPHG---LRLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY-PDSSI 752

Query: 483 VINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQY 542
           V  D+ELQAWW EI+  GH DKK+   WP L T  DL+ I +T+ WV SGHHA+VNF QY
Sbjct: 753 VQTDQELQAWWTEIKTVGHADKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQY 812

Query: 543 AYAGYTPNRPSIARTNILTEDYHQLP-EEFIDLPENELLQVFPSVDQASVVTTTMILLSA 602
           AYAGY P+RP+IARTN+ TED  +   + F+  P++ LLQ FP+  QA+ +   + +LS 
Sbjct: 813 AYAGYFPSRPTIARTNMPTEDPSEEDWKNFVRKPDSALLQCFPTQIQATTIMAVLDILSN 872

Query: 603 HSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPY 662
           HSPDEEYIG+++E AWA EP I  AF+RF+  L  LE+ ID  N +++LKNR+GAGVVPY
Sbjct: 873 HSPDEEYIGEKMEQAWAEEPVIKAAFERFKGRLLALERTIDDRNASSELKNRNGAGVVPY 932

Query: 663 DVLKPTSTYGITGRGVPYSVS 666
           ++LKP S  G+TG+GVPYS+S
Sbjct: 933 ELLKPFSQPGVTGKGVPYSIS 937

BLAST of Cucsa.153660.2 vs. TrEMBL
Match: S4UKU6_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 4.6e-215
Identity = 394/681 (57.86%), Postives = 478/681 (70.19%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           ERK+FERIYDYD YNDLGD D     +RPVLGG + PYPRRCRTGRL C T  + EKRS+
Sbjct: 273 ERKSFERIYDYDVYNDLGDPDKNLRLERPVLGGKEFPYPRRCRTGRLPCDTGSLYEKRSR 332

Query: 63  ERFYVPRDEEFSEVKQHYF-------------PSSEPDNKDL-LGKDSFSDLPHIESMFR 122
           + +YVPRDE FSEVKQ  F             PS E    D  LG   F     I+S+F 
Sbjct: 333 KHWYVPRDEAFSEVKQLTFSAKTLYSVMHALVPSLEIAIADTDLG---FKYFTAIDSLFH 392

Query: 123 EGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDT 182
           EGI+ P        F    ++    P L    +S     +L + PPE+  RD++ W  D 
Sbjct: 393 EGIQLP-------PFKEQGVLKALLPRLVKVMSSG--DDVLRFVPPETMNRDKFFWFRDD 452

Query: 183 EFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIEL 242
           EFARQTLAGLNPYSI+LVT  PL SELDP+ YGP ESA     +++ I     + EAIE 
Sbjct: 453 EFARQTLAGLNPYSIKLVTEWPLKSELDPEIYGPPESAITKEIIEQEIRGFATITEAIEE 512

Query: 243 KRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQW 302
           K+LF+LDYHD  +PYV+KVRK+ GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQW
Sbjct: 513 KKLFILDYHDLFLPYVSKVRKLEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQW 572

Query: 303 KQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHP 362
           KQ+F P   ATD+WLWR+AKAHVLAHDS  HQLV HWLR HC  EPY IATNRQLS +HP
Sbjct: 573 KQVFQPAWNATDVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHP 632

Query: 363 IYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALP 422
           IYRLLHPHFRY M IN+ ARESLINAGGIIE++FS   YS+EL S  Y  +WRFD++ALP
Sbjct: 633 IYRLLHPHFRYTMEINSLARESLINAGGIIETSFSPKKYSLELCSVAYGKEWRFDQEALP 692

Query: 423 EDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENA 482
            DLIRRGMA E     +G   L L I+DYPFANDGLLLW+A+ +WVT+YVNHYY  D + 
Sbjct: 693 ADLIRRGMAVEDPTAPHG---LRLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY-PDSSI 752

Query: 483 VINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQY 542
           V  D+ELQAWW EI+  GH DKK+   WP L T  DL+ I +T+ WV SGHHA+VNF QY
Sbjct: 753 VQTDQELQAWWTEIKTVGHADKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQY 812

Query: 543 AYAGYTPNRPSIARTNILTEDYHQLP-EEFIDLPENELLQVFPSVDQASVVTTTMILLSA 602
           AYAGY P+RP+IARTN+ TED  +   + F+  P++ LLQ FP+  QA+ +   + +LS 
Sbjct: 813 AYAGYFPSRPTIARTNMPTEDPSEEDWKNFVRKPDSALLQCFPTQIQATTIMAVLDILSN 872

Query: 603 HSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPY 662
           HSPDEEYIG+++E AWA EP I  AF+RF+  L  LE+ ID  N +++LKNR+GAGVVPY
Sbjct: 873 HSPDEEYIGEKMEQAWAEEPVIKAAFERFKGRLLALERTIDDRNASSELKNRNGAGVVPY 932

Query: 663 DVLKPTSTYGITGRGVPYSVS 666
           ++LKP S  G+TG+GVPYS+S
Sbjct: 933 ELLKPFSQPGVTGKGVPYSIS 937

BLAST of Cucsa.153660.2 vs. TrEMBL
Match: S4UL90_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6a PE=2 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 5.1e-214
Identity = 393/686 (57.29%), Postives = 473/686 (68.95%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           ERK+FERIYDYD YNDLGD D     +RPVLGG + P+PRRCRTGR  C  DP+SEKRS+
Sbjct: 274 ERKSFERIYDYDVYNDLGDPDKNLRLQRPVLGGKEFPHPRRCRTGRPPCDIDPLSEKRSR 333

Query: 63  ERFYVPRDEEFSEVKQHYF-------------PSSEPDNKDL-LGKDSFSDLPHIESMFR 122
           + +YVPRDE FSEVKQ  F             PS E    D  LG   F     I+S+F 
Sbjct: 334 KHWYVPRDEAFSEVKQLTFSAKTLYSVMHALVPSLEMAIADTNLG---FKYFTAIDSLFH 393

Query: 123 EGIKAPHAPHK-----LLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYS 182
           EGI  P    +     LL   ++ + T D               +L + PPE+  RD++ 
Sbjct: 394 EGIHLPPFKEQGVLKALLPRLVNVMATGD--------------DVLRFVPPETMNRDKFF 453

Query: 183 WLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVN 242
           W  D EF RQTLAGLNPYSI+LVT  PL SELDP  YGP ESA  +  ++  IG    + 
Sbjct: 454 WFRDEEFGRQTLAGLNPYSIKLVTEWPLKSELDPAIYGPPESAITNEIIEREIGGFATIT 513

Query: 243 EAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVD 302
           EAI  K+LF+LDYHD  +PYV+KVRK+ GTTLYGSRTLFFL  + TL PL IELTRPP+D
Sbjct: 514 EAIREKKLFILDYHDLFLPYVSKVRKLEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMD 573

Query: 303 GYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQL 362
           G PQWKQ+F P   +TD+WLWR+AKAHVLAHDS  HQLV HWLR HC  EPY IATNRQL
Sbjct: 574 GKPQWKQVFQPAWNSTDVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQL 633

Query: 363 STVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFD 422
           S +HPIYRLLHPHFRY M IN+ AR+SLINA GIIE++FS   YS+EL S  Y  +WRFD
Sbjct: 634 SVMHPIYRLLHPHFRYTMEINSLARDSLINADGIIETSFSPGKYSLELCSIAYGKEWRFD 693

Query: 423 EQALPEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYG 482
           ++ALP DLIRRGMA E     +G   L L I+DYPFANDGLLLW+A+ +WVT+YVNHYY 
Sbjct: 694 QEALPADLIRRGMAVEDPTAPHG---LRLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY- 753

Query: 483 DDENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASV 542
            D   V  D+ELQAWW EI+  GH DKK+   WP L T  DL+ I +T+ WV SGHHA+V
Sbjct: 754 PDSILVQTDEELQAWWTEIKTVGHADKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAV 813

Query: 543 NFIQYAYAGYTPNRPSIARTNILTEDYHQLP-EEFIDLPENELLQVFPSVDQASVVTTTM 602
           NF QYAY GY PNRP++ARTN+ TED  +   + FI  PE+ LLQ FPS  QA+ +   +
Sbjct: 814 NFGQYAYGGYFPNRPTVARTNVPTEDPSEEDWKNFIKKPESALLQCFPSQIQATRIMAVL 873

Query: 603 ILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGA 662
            +LS HSPDEEYIG+++E AWA EP I  AF+RF+  L  LE  ID  N N++LKNRHGA
Sbjct: 874 DILSNHSPDEEYIGEKMEQAWAEEPVIKAAFERFKGRLLALEGSIDDRNANSELKNRHGA 933

Query: 663 GVVPYDVLKPTSTYGITGRGVPYSVS 666
           GV+PY++LKP S  G+TG+GVPYS+S
Sbjct: 934 GVLPYELLKPFSQPGVTGKGVPYSIS 938

BLAST of Cucsa.153660.2 vs. TrEMBL
Match: S4UL41_MALDO (Lipoxygenase OS=Malus domestica GN=LOX5b PE=2 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 1.2e-210
Identity = 392/681 (57.56%), Postives = 470/681 (69.02%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           +RK  +RIYDYD YND+GD D   E  RPVLGG DHPYPRRCRTGR   K DP+SE+RS 
Sbjct: 249 QRKTSDRIYDYDTYNDIGDPDSKDELARPVLGGKDHPYPRRCRTGRPRSKKDPLSEQRSS 308

Query: 63  ERFYVPRDEEFSEVKQHYF-------------PSSEPDNKDLLGKD-SFSDLPHIESMFR 122
              YVPRDE F++VKQ  F             PS E     LL  D  F     I+S++ 
Sbjct: 309 S-VYVPRDEAFADVKQLTFSTKTLKSVLHALLPSLETA---LLDPDLGFPYFTAIDSLYN 368

Query: 123 EGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDT 182
           EG+  P    K   F  + I    K  +    +      LL +  PE   RD++SW  D 
Sbjct: 369 EGVTLPKP--KTGGFFQTIIPRLVKTIIDGGDD------LLLFETPEIIDRDKFSWFRDE 428

Query: 183 EFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIEL 242
           EF+RQTLAGLNPYSI+LVT  PL S+L+P+ YGP ES      V++ I   + VNEA+E 
Sbjct: 429 EFSRQTLAGLNPYSIELVTEWPLKSKLNPEIYGPPESLITTELVEKEIKGCMTVNEALER 488

Query: 243 KRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQW 302
           K++F+LDYHD LMPYVNKVR+I GTTLYGSRTLFFL +D TL P+ IELTRPPV   PQW
Sbjct: 489 KKMFILDYHDLLMPYVNKVREIEGTTLYGSRTLFFLTADGTLRPVAIELTRPPVGDKPQW 548

Query: 303 KQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHP 362
           KQ+FTP  +AT  WLWR+AKAHVLAHD+  HQLV+HWLR HCC EPY IA NRQLS +HP
Sbjct: 549 KQVFTPTWDATGCWLWRLAKAHVLAHDAGYHQLVVHWLRTHCCTEPYIIAANRQLSAMHP 608

Query: 363 IYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALP 422
           IYRLLHPHFRY M INA ARESLINAGG+IESTFS   YS+ELSS+ Y   WRFD +ALP
Sbjct: 609 IYRLLHPHFRYTMEINALARESLINAGGVIESTFSPGKYSIELSSAAYDQLWRFDMEALP 668

Query: 423 EDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENA 482
            DLIRRGMA E    ++G   L+LAI+DYPFANDGL+LW+A+ EWV++YVNHYY  D N 
Sbjct: 669 ADLIRRGMAVEDSTAEHG---LKLAIEDYPFANDGLILWDAIKEWVSDYVNHYY-TDPNL 728

Query: 483 VINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQY 542
           + +D ELQ WW E++ KGH DKK+   WP LKT  +LI I +TI WV +GHHA+VNF QY
Sbjct: 729 IESDTELQGWWTEVRTKGHADKKDEPWWPVLKTPENLIHILTTIIWVTAGHHAAVNFGQY 788

Query: 543 AYAGYTPNRPSIARTNILTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSA 602
            YAGY PNRP+IARTN+ TED   +  + F+  PE  LL  FPS  QA+ V   + +LS 
Sbjct: 789 MYAGYFPNRPTIARTNMPTEDPSDEYFQNFLKKPEMALLMCFPSQIQATKVMAVLDVLSN 848

Query: 603 HSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPY 662
           HSPDEEYIG   E +WA  P I  A+++F  +LK LE  ID+ N N KLKNR GAGVVPY
Sbjct: 849 HSPDEEYIGGNPESSWAENPVIKAAYEKFNGNLKRLEGIIDERNTNLKLKNRVGAGVVPY 908

Query: 663 DVLKPTSTYGITGRGVPYSVS 666
           ++LKP ST G+TG GVP S+S
Sbjct: 909 ELLKPFSTSGVTGMGVPNSIS 913

BLAST of Cucsa.153660.2 vs. TrEMBL
Match: M5W1M2_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa000968mg PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 2.0e-210
Identity = 392/680 (57.65%), Postives = 470/680 (69.12%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           ERK FERIYDYD YNDLG+ D     +RPVLGG + PYPRRCRTGR  C+TD +SEKRS 
Sbjct: 283 ERKFFERIYDYDVYNDLGEPDKNLRLERPVLGGIEFPYPRRCRTGRPQCETDSLSEKRSN 342

Query: 63  ERFYVPRDEEFSEVKQHYF-------------PSSEPDNKDLLGKDSFSDLPHIESMFRE 122
            ++YVPRDE FSEVKQ  F             PS E    D      F+    I+S+F E
Sbjct: 343 -KWYVPRDEAFSEVKQLTFSAKTVYSVMHALVPSLETAMAD--NDHGFAYFTAIDSLFNE 402

Query: 123 GIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTE 182
           GI  P        F    I+    P L +   S     +L + PPE+  RD++ W  D E
Sbjct: 403 GINLP-------PFKEQGILKTLLPRLVNVVASG--DDVLRFVPPETMNRDKFFWFRDEE 462

Query: 183 FARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELK 242
           FARQTLAGLNP S++LVT+ PL SELDP+ YGP ESA     +++ I     V EAI  K
Sbjct: 463 FARQTLAGLNPCSLKLVTKWPLKSELDPEIYGPPESAITKEIIEQEIRGFPTVQEAIREK 522

Query: 243 RLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWK 302
           +LF+LDYHD  +PYV+KVRKI GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQWK
Sbjct: 523 KLFILDYHDLFLPYVSKVRKIEGTTLYGSRTLFFLTREGTLRPLAIELTRPPMDGKPQWK 582

Query: 303 QIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPI 362
           Q+F P   AT +WLWR+AKAHVLAHDS  HQLV HWLR HC  EPY IAT+RQLS +HPI
Sbjct: 583 QVFQPSWNATGVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCVTEPYIIATSRQLSVMHPI 642

Query: 363 YRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPE 422
           YR LHPHFRY M INA ARESLINAGGIIES+FS   YS+ELS+  Y  +WRFD++ALP 
Sbjct: 643 YRFLHPHFRYTMEINALARESLINAGGIIESSFSPGKYSLELSAVAYGKEWRFDQEALPA 702

Query: 423 DLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAV 482
           DLIRRGMA E     +G   L+L I+DYPFANDGLL+W+A+ +WVT+YVNHYY  D + V
Sbjct: 703 DLIRRGMAVEDPTAPHG---LKLTIEDYPFANDGLLMWDAIKQWVTDYVNHYY-PDSSLV 762

Query: 483 INDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYA 542
             D ELQAWW EI+  GH DKK+   WP L T  DLI I +T+ WV SGHHA+VNF QY 
Sbjct: 763 QTDGELQAWWTEIKTVGHADKKDEPWWPELNTPEDLIGIITTMVWVTSGHHAAVNFGQYV 822

Query: 543 YAGYTPNRPSIARTNILTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAH 602
           YAGY PNRP+IARTN+ TED   +  + F+  PE  LL+ FPS  QA+ +   + +LS H
Sbjct: 823 YAGYFPNRPTIARTNMPTEDPSPEFWKSFLKKPEVALLRCFPSQIQATRIMAVLDILSNH 882

Query: 603 SPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYD 662
           SPDEEYIG+++E AWA EP I  AF+RF+  L  +E  ID  N N++LKNR+GAGVVPY+
Sbjct: 883 SPDEEYIGEKMEAAWAEEPVIKAAFERFKGRLLVIEGIIDDRNANSELKNRNGAGVVPYE 942

Query: 663 VLKPTSTYGITGRGVPYSVS 666
           +LKP S  G+TG GVPYS+S
Sbjct: 943 LLKPFSQPGVTGMGVPYSIS 946

BLAST of Cucsa.153660.2 vs. TAIR10
Match: AT3G45140.1 (AT3G45140.1 lipoxygenase 2)

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-207
Identity = 368/680 (54.12%), Postives = 461/680 (67.79%), Query Frame = 1

Query: 1   MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKR 60
           + E   FERIYDYD YND+GD D   E  RPV+GG  HPYPRRC+TGR  C+TDP SE+R
Sbjct: 231 VGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQR 290

Query: 61  SKERFYVPRDEEFSEVKQHYF---------PSSEPDNKDLL--GKDSFSDLPHIESMFRE 120
               FYVPRDEEFS  K   F         PS  P  + +L   ++ F     I+++F E
Sbjct: 291 YGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEE 350

Query: 121 GIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTE 180
           GI+ P     L          P  P +   +       +L +  P    RDR+SWL D E
Sbjct: 351 GIQLPKDAGLL----------PLLPRI-IKALGEAQDDILQFDAPVLINRDRFSWLRDDE 410

Query: 181 FARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELK 240
           FARQTLAGLNPYSIQLV   PL+S+LDP  YG   S      V+  +  ++ V+EA++ K
Sbjct: 411 FARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNK 470

Query: 241 RLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWK 300
           RLFVLDYHD L+PYVNKVR++N TTLY SRTLFFL  D TL P+ IELT PP    PQWK
Sbjct: 471 RLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWK 530

Query: 301 QIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPI 360
           Q+FTPG +AT  WLW +AK H ++HD+  HQL+ HWLR H C EPY IA NRQLS +HPI
Sbjct: 531 QVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPI 590

Query: 361 YRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPE 420
           YRLLHPHFRY M INA AR+SL+N GGIIE+ F    Y++ELSS+VY   WRFD++ LP 
Sbjct: 591 YRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPA 650

Query: 421 DLIRRGMAER-KKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAV 480
           DLI+RG+AE  K  ++G   + L I DYPFANDGL+LW+A+ EWVT+YV HYY D+E  +
Sbjct: 651 DLIKRGLAEEDKTAEHG---VRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEE-LI 710

Query: 481 INDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYA 540
            +D+ELQ WW+E++  GH DKK+   WP LKT++DLI + +TIAWV SGHHA+VNF QY 
Sbjct: 711 TSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYG 770

Query: 541 YAGYTPNRPSIARTNILTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAH 600
           Y GY PNRP+  R  + TED   +  +EF + PE  LL+ +PS  QA++V  T+ LLS H
Sbjct: 771 YGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTH 830

Query: 601 SPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYD 660
           SPDEEYIG++ E +WA EP I+ AF+RF+  L+ LE  ID+ N N  LKNR GAGVV Y+
Sbjct: 831 SPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYE 890

Query: 661 VLKPTSTYGITGRGVPYSVS 666
           +LKPTS +G+TG GVPYS+S
Sbjct: 891 LLKPTSEHGVTGMGVPYSIS 895

BLAST of Cucsa.153660.2 vs. TAIR10
Match: AT1G67560.1 (AT1G67560.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 570.9 bits (1470), Expect = 1.1e-162
Identity = 315/694 (45.39%), Postives = 421/694 (60.66%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           ERK  ERIYDYD YNDLGD     E  RPVLG  + PYPRRCRTGR     DP  E R K
Sbjct: 247 ERKPHERIYDYDVYNDLGD-PRKTERVRPVLGVPETPYPRRCRTGRPLVSKDPPCESRGK 306

Query: 63  ER--FYVPRDEEFSEVKQHYF-------------PS--SEPDNKDLLGKDSFSDLPHIES 122
           E+  FYVPRDE F E+K+  F             PS  +   N D+     F+    I++
Sbjct: 307 EKEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSIAAALSNLDI----PFTCFSDIDN 366

Query: 123 MFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWL 182
           +++  I   H   K     L   +         N   +V  +LL Y  P   + DR++WL
Sbjct: 367 LYKSNIVLGHTEPK--DTGLGGFIGG-----FMNGILNVTETLLKYDTPAVIKWDRFAWL 426

Query: 183 SDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIE---- 242
            D EF RQ LAG+NP +I+L+  LP+ S LDP  YGPQES       +E+I   +E    
Sbjct: 427 RDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLT----EEIIAREVEHYGT 486

Query: 243 -VNEAIELKRLFVLDYHDTLMPYVNKVRKI--NGTTLYGSRTLFFLKSDDTLIPLGIELT 302
            + +A+E KRLF++DYHD L+P+V K+  I  +    Y SRT+FF   +  L PL IEL+
Sbjct: 487 TIEKALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELS 546

Query: 303 RPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIA 362
            PP     + K ++T G +AT  W+W++AKAHV ++D+ +HQLV HWLR H  MEPY IA
Sbjct: 547 LPPT-AESENKFVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIA 606

Query: 363 TNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRD 422
           TNRQLST+HP+Y+LLHPH RY + INA AR+SLIN GGIIES F+   Y++ELSS+ Y+ 
Sbjct: 607 TNRQLSTMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKS 666

Query: 423 QWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVN 482
            WRFD + LP DL+RRGMAE          + L I DYP+A DGLL+W A+ + V  YV 
Sbjct: 667 MWRFDMEGLPADLVRRGMAEEDSS--AECGVRLVIDDYPYAADGLLIWKAIKDLVESYVK 726

Query: 483 HYYGDDENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGH 542
           H+Y D + ++ +D ELQAWW+EI+ KGH DKK+   WP L T  DL +I + + W+ SG 
Sbjct: 727 HFYSDSK-SITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQ 786

Query: 543 HASVNFIQYAYAGYTPNRPSIARTNILTE---DYHQLPEEFIDLPENELLQVFPSVDQAS 602
           HA++NF QY + GY PNRP++ R  I  E   DY    E F+  P+   L   P+  QA+
Sbjct: 787 HAAINFGQYPFGGYVPNRPTLLRKLIPQETDPDY----EMFMRNPQYSFLGSLPTQLQAT 846

Query: 603 VVTTTMILLSAHSPDEEYIGD--EIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNN 662
            V      LS HSPDEEY+ +  E++  W  +  + K F +F   L  +E+ I++ NK+ 
Sbjct: 847 KVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKDK 906

Query: 663 KLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 666
           KLKNR GAG+ PY++L PTS +G+TGRG+P S+S
Sbjct: 907 KLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSIS 916

BLAST of Cucsa.153660.2 vs. TAIR10
Match: AT1G72520.1 (AT1G72520.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 548.5 bits (1412), Expect = 5.6e-156
Identity = 295/682 (43.26%), Postives = 418/682 (61.29%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSK 62
           ERK  +RIYDYD YND+G+ D   E  RP LGG + PYPRRCRTGR    TD +SE+R +
Sbjct: 259 ERKLSDRIYDYDVYNDIGNPDISRELARPTLGGREFPYPRRCRTGRSSTDTDMMSERRVE 318

Query: 63  ERF--YVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKDSFSDLPHIESMFREGI 122
           +    YVPRDE+F E KQ+ F +           P  K  +  + F++   I+S+++EG+
Sbjct: 319 KPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKASILAEDFANFGEIDSLYKEGL 378

Query: 123 --KAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTE 182
             K          F L  IVT     L  +S       LL Y  P+   +D+Y+WL D E
Sbjct: 379 LLKLGFQDDMFKKFPLPKIVTT----LQKSSEG-----LLRYDTPKIVSKDKYAWLRDDE 438

Query: 183 FARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQ-ESAFNHTKVQELIGCSIEVNEAIEL 242
           FARQ +AG+NP +I+ VT  P +S LDP+ YGP   SA     +   +   + V +A+E 
Sbjct: 439 FARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQLD-GLTVQQALET 498

Query: 243 KRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQW 302
            RLF++DYHD  +P+++++  ++G   Y +RT+ FL    TL P+ IEL+ P      Q 
Sbjct: 499 NRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLPSQSSSNQK 558

Query: 303 -KQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVH 362
            K++ TP ++AT  W+W++AKAHV ++D+ +HQLV HWLR H C+EP+ +A +RQLS +H
Sbjct: 559 SKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMH 618

Query: 363 PIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQAL 422
           PI++LL PH RY + INA AR++LI+A G+IES F+   Y +E+SS+ Y+++WRFD + L
Sbjct: 619 PIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFDMEGL 678

Query: 423 PEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDEN 482
           P DLIRRGMA       +G   L+L ++DYP+ANDGLLLW+A+  WV  YV  YY  + N
Sbjct: 679 PADLIRRGMAVPDPTQPHG---LKLLVEDYPYANDGLLLWSAIQTWVRTYVERYYA-NSN 738

Query: 483 AVINDKELQAWWNEIQEKGHPDKK--EGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQ 542
            +  D ELQAW++E    GH D +  E WP L T  DL+ + +TI W+ S  HA++NF Q
Sbjct: 739 LIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQ 798

Query: 543 YAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSA 602
           Y Y GY PNRP + R  I  E   +    FI+ P+       PS+ Q +     +  LS 
Sbjct: 799 YPYGGYVPNRPPLMRRLIPDESDPEF-TSFIEDPQKYFFSSMPSLLQTTKFMAVVDTLST 858

Query: 603 HSPDEEYIGDEIEPA-WALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVP 662
           HSPDEEYIG+  +P+ W  +  I  AF  F A +  +E++IDK N++   +NR GAGV+P
Sbjct: 859 HSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNRCGAGVLP 918

Query: 663 YDVLKPTSTYGITGRGVPYSVS 666
           Y+++ P+S  G+T RGVP SVS
Sbjct: 919 YELMAPSSEPGVTCRGVPNSVS 925

BLAST of Cucsa.153660.2 vs. TAIR10
Match: AT1G17420.1 (AT1G17420.1 lipoxygenase 3)

HSP 1 Score: 541.6 bits (1394), Expect = 6.9e-154
Identity = 287/678 (42.33%), Postives = 412/678 (60.77%), Query Frame = 1

Query: 4   RKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKE 63
           RK  +RIYD+D YNDLG+ D  +E  RP LGG + PYPRRCRTGR    +D  +E R ++
Sbjct: 254 RKLSDRIYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRRCRTGRQSTVSDKDAESRVEK 313

Query: 64  RF--YVPRDEEFSEVKQHYFPSSE---------PDNKDLLGKDSFSDLPHIESMFREGIK 123
               YVPRDE+F E KQ  F +           P  K  +  + F+D   I+ +++EG+ 
Sbjct: 314 PLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGLL 373

Query: 124 APHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFAR 183
                   L F          P +  ++       LL Y  P+   +D+ +WL D EFAR
Sbjct: 374 LK------LGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFAR 433

Query: 184 QTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKV-QELIGCSIEVNEAIELKRL 243
           Q +AG+NP +I+ V   P +S LDP  YGPQ SA     +   L G S++  +A+E  RL
Sbjct: 434 QAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDGFSVQ--QALEENRL 493

Query: 244 FVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQI 303
           ++LDYHD  +P+++++  ++G   Y +RT+FFL    TL P+ IEL+ PP     + K++
Sbjct: 494 YMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLPPHGPKHRSKRV 553

Query: 304 FTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYR 363
            TP ++AT  W+W++AKAHV ++D+ +HQLV HWLR H C+EP+ +A +RQLS +HPI++
Sbjct: 554 LTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFK 613

Query: 364 LLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDL 423
           LL PH RY + INA AR+SLI+A G+IE  F+  +Y +E+S++ Y+  WRFD + LP DL
Sbjct: 614 LLDPHMRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADL 673

Query: 424 IRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVIN 483
           IRRGMA       +G   L+L I+DYP+ANDGLLLW+A+  WV  YV  YY  + N +  
Sbjct: 674 IRRGMAIPDATQPHG---LKLLIEDYPYANDGLLLWSAIQTWVRTYVERYY-PNPNLIKT 733

Query: 484 DKELQAWWNEIQEKGHPDKKEG--WPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYA 543
           D ELQ+W++E    GH D ++   WP L T +DL+ I +T+ W+ S  HA++NF QY Y 
Sbjct: 734 DSELQSWYSESINVGHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYG 793

Query: 544 GYTPNRPSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPD 603
           GY PNRP + R  ++ ++       FI  PE       PS+ Q S     +  LS HSPD
Sbjct: 794 GYVPNRPPLMR-RLIPDESDPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPD 853

Query: 604 EEYIGDEIEPA-WALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVL 663
           EEYIG+  +P+ W  +  I +AF  F A +  +E++I+K N +   +NR GAGV+PY++L
Sbjct: 854 EEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLPYELL 913

Query: 664 KPTSTYGITGRGVPYSVS 666
            P+S  G+T RGVP SVS
Sbjct: 914 VPSSEPGVTCRGVPNSVS 918

BLAST of Cucsa.153660.2 vs. TAIR10
Match: AT1G55020.1 (AT1G55020.1 lipoxygenase 1)

HSP 1 Score: 494.6 bits (1272), Expect = 9.7e-140
Identity = 288/687 (41.92%), Postives = 407/687 (59.24%), Query Frame = 1

Query: 3   ERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGS-DHPYPRRCRTGRLHCKTDPVSEKR- 62
           E K ++R+YDY +YNDLG    P +  RPVLGG+ ++PYPRR RTGR   K DP +E R 
Sbjct: 192 ELKEWDRVYDYAYYNDLGV---PPKNPRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRL 251

Query: 63  ---SKERFYVPRDEEFSEVKQHYF-------------PSSEPDNKDLLGK-DSFSDLPHI 122
              S    YVPRDE F  +K   F             P+ E    D   + DSF D+  I
Sbjct: 252 PITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKI 311

Query: 123 ESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYS 182
              + EGI  P+         + +IV     ++      +     L +P P+  + D+ +
Sbjct: 312 ---YEEGIDLPNQAL------IDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTA 371

Query: 183 WLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVN 242
           W +D EFAR+ LAGLNP  IQL+   P  S+LD ++YG Q S    + ++  +   + V 
Sbjct: 372 WRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLD-GLTVE 431

Query: 243 EAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVD 302
           EA+E +RLF+LD+HDTLMPY+ +V     T  Y SRTL FLK D TL PL IEL+ P  +
Sbjct: 432 EALEKERLFILDHHDTLMPYLGRVNTTT-TKTYASRTLLFLKDDGTLKPLVIELSLPHPN 491

Query: 303 G--YPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNR 362
           G  +    +++TPG    D  LW++AKA V  +DS  HQL+ HW++ H  +EP+ IATNR
Sbjct: 492 GDKFGAVSEVYTPGEGVYDS-LWQLAKAFVGVNDSGNHQLISHWMQTHASIEPFVIATNR 551

Query: 363 QLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWR 422
           QLS +HP+++LL PHFR  M INA AR+ LIN GGI E T   + Y++E+SS +Y++ W 
Sbjct: 552 QLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMSSFIYKNHWT 611

Query: 423 FDEQALPEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHY 482
           F +QALP +L +RGMA E  +  +G   L L IKDYP+A DGL +W A+  WV +Y+  +
Sbjct: 612 FPDQALPAELKKRGMAVEDPEAPHG---LRLRIKDYPYAVDGLEVWYAIESWVRDYIFLF 671

Query: 483 YGDDENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHA 542
           Y  +E+ +  D ELQAWW E++E+GH DKK    WP ++TR +L++  + I WV S  HA
Sbjct: 672 YKIEED-IQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIIIWVASALHA 731

Query: 543 SVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTT 602
           +VNF QY  AGY PNRP+I+R  +  E+  +  EE    P+   L+   +  Q  +  + 
Sbjct: 732 AVNFGQYPVAGYLPNRPTISRQYMPKENTPEF-EELEKNPDKVFLKTITAQLQTLLGISL 791

Query: 603 MILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHG 662
           + +LS HS DE Y+G      WA E    +AF++F   +K++E+ ID+ N +  LKNR G
Sbjct: 792 IEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRTG 851

Query: 663 AGVVPYDVLKPTSTYGITGRGVPYSVS 666
              +PY +L P+S  G+TGRG+P SVS
Sbjct: 852 LVKMPYTLLFPSSEGGVTGRGIPNSVS 858

BLAST of Cucsa.153660.2 vs. NCBI nr
Match: gi|449448968|ref|XP_004142237.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 664/666 (99.70%), Postives = 666/666 (100.00%), Query Frame = 1

Query: 1   MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKR 60
           MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKR
Sbjct: 165 MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKR 224

Query: 61  SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL 120
           SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL
Sbjct: 225 SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL 284

Query: 121 LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNP 180
           LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNP
Sbjct: 285 LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNP 344

Query: 181 YSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTL 240
           YSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTL
Sbjct: 345 YSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTL 404

Query: 241 MPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATD 300
           MPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATD
Sbjct: 405 MPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATD 464

Query: 301 LWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYN 360
           LWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYN
Sbjct: 465 LWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYN 524

Query: 361 MRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERK 420
           MRINANARESLINAGGIIESTFSTASYSVELSSS+YRDQWRFDEQALPEDLIRRGMAERK
Sbjct: 525 MRINANARESLINAGGIIESTFSTASYSVELSSSMYRDQWRFDEQALPEDLIRRGMAERK 584

Query: 421 KGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNE 480
           KGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNE
Sbjct: 585 KGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNE 644

Query: 481 IQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIART 540
           IQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIART
Sbjct: 645 IQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIART 704

Query: 541 NILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAW 600
           NILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAW
Sbjct: 705 NILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAW 764

Query: 601 ALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGV 660
           ALEPSISKAFKRFQASLKDLEQQID+NNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGV
Sbjct: 765 ALEPSISKAFKRFQASLKDLEQQIDENNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGV 824

Query: 661 PYSVST 667
           PYSVST
Sbjct: 825 PYSVST 830

BLAST of Cucsa.153660.2 vs. NCBI nr
Match: gi|659097677|ref|XP_008449754.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 623/667 (93.40%), Postives = 641/667 (96.10%), Query Frame = 1

Query: 1   MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKR 60
           MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGS+ PYPRRCRTGRLHC  DPVSEKR
Sbjct: 165 MNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSERPYPRRCRTGRLHCIADPVSEKR 224

Query: 61  SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL 120
           SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGK+SF DLPHIESMFREGIKAP APHKL
Sbjct: 225 SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKNSFHDLPHIESMFREGIKAPDAPHKL 284

Query: 121 LTFNLSTIVTPD-KPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLN 180
           L FNLSTIVTPD KPDLHSNS  SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLN
Sbjct: 285 LKFNLSTIVTPDHKPDLHSNS--SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLN 344

Query: 181 PYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDT 240
           PYSIQLVTRLPLMSELDP+ YGPQESAFNHTKVQEL+GC  EVNEAIE KRLFV+DYHDT
Sbjct: 345 PYSIQLVTRLPLMSELDPEIYGPQESAFNHTKVQELLGCFNEVNEAIECKRLFVVDYHDT 404

Query: 241 LMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEAT 300
           LMPYVNKVR INGTTLYGSRTLFFLKSD TLIPLGIELTRPPVDGYPQWKQIFTPG EAT
Sbjct: 405 LMPYVNKVRTINGTTLYGSRTLFFLKSDGTLIPLGIELTRPPVDGYPQWKQIFTPGTEAT 464

Query: 301 DLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRY 360
           D+WLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLST+HPIYRLLHPHFRY
Sbjct: 465 DVWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTLHPIYRLLHPHFRY 524

Query: 361 NMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAER 420
           NMRINANARESLINAGGIIE TFSTASYS+ELSSSVYRDQWRFDEQA PEDLIRRGMAER
Sbjct: 525 NMRINANARESLINAGGIIEGTFSTASYSMELSSSVYRDQWRFDEQAFPEDLIRRGMAER 584

Query: 421 KKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWN 480
           KK +YGRD+LEL IKDYPFANDGL+LWNALLEWVTEYVNHYYGDDENAVINDKELQAWWN
Sbjct: 585 KKDEYGRDILELTIKDYPFANDGLILWNALLEWVTEYVNHYYGDDENAVINDKELQAWWN 644

Query: 481 EIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIAR 540
           EIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIAR
Sbjct: 645 EIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIAR 704

Query: 541 TNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPA 600
           TNILTEDYHQLPEEFIDLPEN LLQVFPSVDQA++VTTTMILLSAHSPDEEYIG E+EPA
Sbjct: 705 TNILTEDYHQLPEEFIDLPENVLLQVFPSVDQATMVTTTMILLSAHSPDEEYIGVEVEPA 764

Query: 601 WALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRG 660
           WALEP+I KAFKRFQA+LKDLEQQID+NNKN  LKNRHGAGVVPY+VLKPTST GITGRG
Sbjct: 765 WALEPAIDKAFKRFQANLKDLEQQIDENNKNKSLKNRHGAGVVPYEVLKPTSTDGITGRG 824

Query: 661 VPYSVST 667
           VPYSVST
Sbjct: 825 VPYSVST 829

BLAST of Cucsa.153660.2 vs. NCBI nr
Match: gi|778693100|ref|XP_011653578.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 960.3 bits (2481), Expect = 1.8e-276
Identity = 475/668 (71.11%), Postives = 554/668 (82.93%), Query Frame = 1

Query: 2   NERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSD-HPYPRRCRTGRLHCKTDPVSEKR 61
           NERKAFERIYDYD YNDLGD D     KRPVLGGS+ +PYPRRCRTGR   + DP SEKR
Sbjct: 164 NERKAFERIYDYDLYNDLGDPDQSMNLKRPVLGGSEEYPYPRRCRTGRPLTQNDPASEKR 223

Query: 62  SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL 121
            +E FYVPRDEEFSE+KQ    SS+P N+ LL K  FSDLP +E      IK P A  K 
Sbjct: 224 IEEWFYVPRDEEFSEIKQS---SSQPGNEKLLRKSPFSDLPELE------IKTPSAASKF 283

Query: 122 -LTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLN 181
            L FN+++I++  +P    +S++   S+L+  PPPESY+RD+Y+WLSD EFAR TL GLN
Sbjct: 284 NLQFNIASILSSHRPPALKSSDTP-SSTLVELPPPESYKRDKYNWLSDIEFARLTLVGLN 343

Query: 182 PYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDT 241
           PYSIQLV  LP MS+LD   Y P+ES F    VQEL+GC I V++A+  KRLFV+DYHDT
Sbjct: 344 PYSIQLVKSLPFMSKLDEGDYRPRESKFTPEXVQELLGCYITVDKALADKRLFVVDYHDT 403

Query: 242 LMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGY-PQWKQIFTPGIEA 301
           LMPYV+KVRKI GTTLYGSRTLFFL SD  L+PLGIELTRPP+DG+  QWK++FTPG  +
Sbjct: 404 LMPYVSKVRKIKGTTLYGSRTLFFLNSDGILMPLGIELTRPPMDGHLEQWKEVFTPGTNS 463

Query: 302 TDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFR 361
           TD+WLWR+AKAHVL+HDSCIHQLVIHWLRAHCCME YAIATNRQLST+HPIYRLLHPHFR
Sbjct: 464 TDVWLWRLAKAHVLSHDSCIHQLVIHWLRAHCCMESYAIATNRQLSTMHPIYRLLHPHFR 523

Query: 362 YNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAE 421
           YNMRINANAR++LINA GIIE+ FS ASYSVELSSS Y+  WRFDEQALPEDLI RGMAE
Sbjct: 524 YNMRINANARKNLINARGIIENAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAE 583

Query: 422 RKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWW 481
           RK+   GRDVLEL IKDYPFANDGLLLWNALLEWVTEYVNHYYGD+ENAV NDKELQAWW
Sbjct: 584 RKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWW 643

Query: 482 NEIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIA 541
            EIQEKGHPDK+EGWP L+TR+DLIKIASTIAWVGSGHHASVNF+QYAY GY PNRP+IA
Sbjct: 644 GEIQEKGHPDKREGWPALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIA 703

Query: 542 RTNILTEDY-HQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIE 601
           RTN+LTE++  ++ ++F + PEN+L ++FPSV QA++V  TM LLS HSPDEEYIGD IE
Sbjct: 704 RTNMLTENHSDEILKDFRNYPENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIE 763

Query: 602 PAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITG 661
           PAWAL+ SISKAF++F+A+L DLE++ID+ N+N  LKNR+GAG++PY+V+KP S  G+TG
Sbjct: 764 PAWALDQSISKAFEKFKANLTDLEKKIDELNENKDLKNRYGAGIIPYEVMKPRSKPGVTG 820

Query: 662 RGVPYSVS 666
            GVPYSVS
Sbjct: 824 IGVPYSVS 820

BLAST of Cucsa.153660.2 vs. NCBI nr
Match: gi|778693093|ref|XP_004142238.2| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 959.1 bits (2478), Expect = 3.9e-276
Identity = 478/669 (71.45%), Postives = 552/669 (82.51%), Query Frame = 1

Query: 2   NERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSD-HPYPRRCRTGRLHCKTDPVSEKR 61
           NERKAFERIYDYD YNDLGD D   + KRPVLGGS+ +PYPRRCRTGR   + DP SEKR
Sbjct: 164 NERKAFERIYDYDLYNDLGDPDLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKR 223

Query: 62  SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL 121
            +E FY+PRDEEFSE+KQ    S++P NK LL K   SDLP I      GIK P A  K+
Sbjct: 224 VEEWFYIPRDEEFSEIKQS---SNQPRNKKLLKKAPSSDLPEI------GIKMPSAASKI 283

Query: 122 -LTFNLSTIVTPDKPDLHSNSNSSVPSS-LLHYPPPESYRRDRYSWLSDTEFARQTLAGL 181
            L  N+S+IV   +P   + ++S VPSS L+  PPPESY+RD+Y+WLSD EFAR TLAGL
Sbjct: 284 NLQCNVSSIVCSHQPPAAAINSSDVPSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGL 343

Query: 182 NPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHD 241
           NPYSIQLV  LP MS+LD   YGP+ES F   +VQEL+GC I V +A+  KRLFV+DYHD
Sbjct: 344 NPYSIQLVKSLPFMSKLDEGDYGPRESKFTPERVQELLGCCITVGKALADKRLFVVDYHD 403

Query: 242 TLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGY-PQWKQIFTPGIE 301
           TLMPYV +VRKI GTTLYGSRTLFFL SD TL+PLGIELTRPP+D +  QWK++FTPG  
Sbjct: 404 TLMPYVRRVRKIKGTTLYGSRTLFFLNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTN 463

Query: 302 ATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHF 361
           +TDLWLWR AKAHVL+HDSCIHQLVIHWLRAHCCMEPYAIATNRQLST+HPIYRLLHPHF
Sbjct: 464 STDLWLWRFAKAHVLSHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHF 523

Query: 362 RYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA 421
           RYNMRINANAR++LINAGGIIE+ FS ASYSVELSSS Y++ WRFD QALPEDLI RGMA
Sbjct: 524 RYNMRINANARKNLINAGGIIENAFSAASYSVELSSSAYKE-WRFDMQALPEDLIHRGMA 583

Query: 422 ERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAW 481
           ERK+   GRDVLEL IKDYPFANDGLLLWNALLEWVTEYVNHYYGD+ENAVIND ELQAW
Sbjct: 584 ERKRDSEGRDVLELTIKDYPFANDGLLLWNALLEWVTEYVNHYYGDEENAVINDTELQAW 643

Query: 482 WNEIQEKGHPDKKEGWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSI 541
           W EIQEKGHPDK+EGW  L+TR+DLIKIASTIAWVGSGHHASVNF+QYAY GY PNRP+I
Sbjct: 644 WGEIQEKGHPDKREGWFALRTRDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTI 703

Query: 542 ARTNILTEDY-HQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEI 601
           ARTN+LTE++  +  ++FI+ PE +L ++FPS  QA++V  TM LLS HSPDEEYIGD I
Sbjct: 704 ARTNMLTENHSDKFLKDFINQPEKKLNELFPSDAQAAIVKQTMFLLSIHSPDEEYIGDAI 763

Query: 602 EPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGIT 661
           EPAWAL+ SISKAF++F+A+L DLE++ID+ N+N  LKNR+GA ++PY+ +KP S  GIT
Sbjct: 764 EPAWALDQSISKAFEKFKANLTDLEKKIDELNQNKDLKNRYGAAIIPYEAMKPRSKPGIT 822

Query: 662 GRGVPYSVS 666
           G GVPYSVS
Sbjct: 824 GSGVPYSVS 822

BLAST of Cucsa.153660.2 vs. NCBI nr
Match: gi|778693086|ref|XP_004142240.2| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 927.5 bits (2396), Expect = 1.3e-266
Identity = 458/672 (68.15%), Postives = 536/672 (79.76%), Query Frame = 1

Query: 2   NERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGS-DHPYPRRCRTGRLHCKTDPVSEKR 61
           NERKAFERIYDYD YNDLGD D   +WKRPVLGGS  +PYPRRCRTGR    +DP SE+R
Sbjct: 164 NERKAFERIYDYDVYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPATVSDPKSEQR 223

Query: 62  SKERFYVPRDEEFSEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL 121
           S E FYVPRDE FSEVKQ  FPS+ P  KD LG   F+D   I+ MFR+GI  P  PH++
Sbjct: 224 STESFYVPRDEAFSEVKQKMFPSN-PGKKDKLGAKPFTDFTQIDLMFRDGIAPPSNPHEI 283

Query: 122 LTFNL-STIVTPDKPDLHSNSNSSVPSS--LLHYPPPESYRRDRYSWLSDTEFARQTLAG 181
           L FN+ ST+   ++P         V  +  ++ +PPPE+ +RD+++WLSD+EFARQT+AG
Sbjct: 284 LQFNIISTLNASNQPAPPPVQPIPVQPNTPVIKFPPPEALKRDKFNWLSDSEFARQTIAG 343

Query: 182 LNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYH 241
           LNPY IQLV   PL SELDP  YGPQESAF    VQ+LIG SI V EAI  K+LFVLDYH
Sbjct: 344 LNPYCIQLVKSWPLKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYH 403

Query: 242 DTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIE 301
           DTLM YV KVR I  TTLYGSRTLFFL  DDTL PLGIELTRPP+ G PQWKQ+F+P  +
Sbjct: 404 DTLMQYVEKVRSIKRTTLYGSRTLFFLNDDDTLSPLGIELTRPPMAGKPQWKQVFSPSTQ 463

Query: 302 ATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHF 361
           AT+ WLWR+AKAHVLAHDSC+HQLVIHWLR HCCMEPYAIA NRQLST+HPIYRLLHPHF
Sbjct: 464 ATNAWLWRLAKAHVLAHDSCVHQLVIHWLRTHCCMEPYAIALNRQLSTMHPIYRLLHPHF 523

Query: 362 RYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA 421
           R+NMRIN+NARE LINAGGIIESTFS   YS+E SS VY+ +W+FD QALPEDLIRRGMA
Sbjct: 524 RFNMRINSNAREILINAGGIIESTFSAGGYSMEFSSLVYKKEWQFDTQALPEDLIRRGMA 583

Query: 422 ER-KKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQA 481
           ER     +G   L+LAIKDYPFANDGL+LW AL+EW++EYVNHYY  DE A+ NDKEL+A
Sbjct: 584 ERDASAPHG---LKLAIKDYPFANDGLILWEALVEWISEYVNHYY-PDEKAIKNDKELKA 643

Query: 482 WWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNR 541
           WW EI EKGHPDKK+  GWP+LKT NDLI+I STIAWVG GHH++VNFIQYA+AGY PNR
Sbjct: 644 WWKEIMEKGHPDKKKEAGWPSLKTPNDLIQIVSTIAWVGCGHHSAVNFIQYAHAGYFPNR 703

Query: 542 PSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGD 601
           PSI RTN+ TEDY Q+PE FI+ PE+ +L+ FPS+ QAS V  TM++LSAHSPDEE IG 
Sbjct: 704 PSITRTNMPTEDYDQIPEAFINNPESVILEAFPSIAQASTVAQTMLILSAHSPDEEDIGK 763

Query: 602 EIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYG 661
           +IEPAWA +P I+KAF +F+  L  LE+ ID+ N+NN+LKNR GAG+VPY+VLKPTS +G
Sbjct: 764 KIEPAWAEDPMIAKAFDKFKMKLDKLEKTIDQRNENNELKNRRGAGLVPYEVLKPTSGFG 823

Query: 662 ITGRGVPYSVST 667
           +TG+GVPYSVST
Sbjct: 824 VTGKGVPYSVST 830