Cucsa.153660 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.153660 vs. Swiss-Prot
Match: LOX2_ARATH (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana GN=LOX2 PE=1 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 4.6e-226
Identity = 408/784 (52.04%), Postives = 520/784 (66.33%), Query Frame = 1

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           +KY  E E+PE +G +GA+ ++      +Y + +    +  K    S TF+C+SWV  KS
Sbjct: 135 EKYECEFEMPEDFGPVGAIKIQ-----NQYHRQLFLKGVELKLPGGSITFTCESWVAPKS 194

Query: 121 VLDQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFY 180
           V   +R+FFS KSYLP +TP  L K R E+L  L+G   +E   + E   FERIYDYD Y
Sbjct: 195 VDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREE---VGEFTKFERIYDYDVY 254

Query: 181 NDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEV 240
           ND+GD D   E  RPV+GG  HPYPRRC+TGR  C+TDP SE+R    FYVPRDEEFS  
Sbjct: 255 NDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTA 314

Query: 241 KQHYF---------PSSEPDNKDLL--GKDSFSDLPHIESMFREGIKAPHAPHKLLTFNL 300
           K   F         PS  P  + +L   ++ F     I+++F EGI+ P     L     
Sbjct: 315 KGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLL----- 374

Query: 301 STIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQL 360
                P  P +   +       +L +  P    RDR+SWL D EFARQTLAGLNPYSIQL
Sbjct: 375 -----PLLPRI-IKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQL 434

Query: 361 VTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVN 420
           V   PL+S+LDP  YG   S      V+  +  ++ V+EA++ KRLFVLDYHD L+PYVN
Sbjct: 435 VEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVN 494

Query: 421 KVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWR 480
           KVR++N TTLY SRTLFFL  D TL P+ IELT PP    PQWKQ+FTPG +AT  WLW 
Sbjct: 495 KVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWN 554

Query: 481 IAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINA 540
           +AK H ++HD+  HQL+ HWLR H C EPY IA NRQLS +HPIYRLLHPHFRY M INA
Sbjct: 555 LAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINA 614

Query: 541 NARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAER-KKGDY 600
            AR+SL+N GGIIE+ F    Y++ELSS+VY   WRFD++ LP DLI+RG+AE  K  ++
Sbjct: 615 RARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEH 674

Query: 601 GRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEK 660
           G   + L I DYPFANDGL+LW+A+ EWVT+YV HYY D+E  + +D+ELQ WW+E++  
Sbjct: 675 G---VRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEE-LITSDEELQGWWSEVRNI 734

Query: 661 GHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNI 720
           GH DKK+   WP LKT++DLI + +TIAWV SGHHA+VNF QY Y GY PNRP+  R  +
Sbjct: 735 GHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRM 794

Query: 721 LTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWA 780
            TED   +  +EF + PE  LL+ +PS  QA++V  T+ LLS HSPDEEYIG++ E +WA
Sbjct: 795 PTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWA 854

Query: 781 LEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVP 830
            EP I+ AF+RF+  L+ LE  ID+ N N  LKNR GAGVV Y++LKPTS +G+TG GVP
Sbjct: 855 NEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVP 895

BLAST of Cucsa.153660 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 9.1e-222
Identity = 410/785 (52.23%), Postives = 525/785 (66.88%), Query Frame = 1

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  +  +P+ +GE+GA+++E E     Y K I    + D         +C SWV SK   
Sbjct: 137 YEADFVIPQDFGEVGAILIENEHHKEMYVKNI----VIDGFVHGKVEITCNSWVHSKFDN 196

Query: 123 DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYND 182
             +R+FF+ KSYLP +TP G+ +LR E+L  LRG        + ERK FERIYDYD YND
Sbjct: 197 PDKRIFFTNKSYLPSQTPSGVSRLREEELVTLRGD------GIGERKVFERIYDYDVYND 256

Query: 183 LGDCDGPAE-WKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVK 242
           LG+ D   +  KRPVLGG + PYPRRC+TGR   K DP+SE RS    YVPRDE FSEVK
Sbjct: 257 LGEADSNNDDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETRSTF-VYVPRDEAFSEVK 316

Query: 243 QHYFPSSE---------PDNKDLLGKDS--FSDLPHIESMFREGIKAPHAPHKLL-TFNL 302
              F  +          P  + ++   +  F   P I+S+F  G+  P    K    FN 
Sbjct: 317 SVAFSGNTVYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFNVGVDLPGLGDKKSGLFN- 376

Query: 303 STIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQL 362
              V P      S++   V    L +  P+  +RD++SW  D EFARQTLAGLNPYSI+L
Sbjct: 377 ---VVPRLIKAISDTRKDV----LLFESPQLVQRDKFSWFRDVEFARQTLAGLNPYSIRL 436

Query: 363 VTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVN 422
           VT  PL S+LDP  YGP ES      +++ IG  + V +A++ K+LF+LDYHD L+PYVN
Sbjct: 437 VTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQAVQQKKLFILDYHDLLLPYVN 496

Query: 423 KVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGI-EATDLWLW 482
           KV ++ G+ LYGSRT+FFL    TL PL IELTRPPVD  PQWK++++P    AT  WLW
Sbjct: 497 KVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDKPQWKEVYSPNDWNATGAWLW 556

Query: 483 RIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRIN 542
           ++AKAHVL+HDS  HQLV HWLR HCC EPY IA+NRQLS +HPIYRLLHPHFRY M IN
Sbjct: 557 KLAKAHVLSHDSGYHQLVSHWLRTHCCTEPYIIASNRQLSAMHPIYRLLHPHFRYTMEIN 616

Query: 543 ANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGD 602
           A ARE+LINA G+IES+F    Y++ELSS  Y  +WRFD++ALP++LI RG+A E     
Sbjct: 617 ALAREALINANGVIESSFFPGKYAIELSSIAYGAEWRFDQEALPQNLISRGLAVEDPNEP 676

Query: 603 YGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQE 662
           +G   L+LAI+DYPFANDGL+LW+ L +WVT YVNHYY    N + +DKELQAWW+EI+ 
Sbjct: 677 HG---LKLAIEDYPFANDGLVLWDILKQWVTNYVNHYY-PQTNLIESDKELQAWWSEIKN 736

Query: 663 KGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTN 722
            GH DK++   WP LKT NDLI I +TI WV SGHHA+VNF QY+YAGY PNRP++AR+ 
Sbjct: 737 VGHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSK 796

Query: 723 ILTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAW 782
           + TED   +  E F++ PE  LL+ FPS  QA+ V   + +LS HSPDEEYIG++IEP W
Sbjct: 797 MPTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYW 856

Query: 783 ALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGV 830
           A +P I+ AF+ F   LK+LE  ID  N ++KL NR+GAGV+PY++LKP S  G+TG+GV
Sbjct: 857 AEDPVINAAFEVFSGKLKELEGIIDARNNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGV 898

BLAST of Cucsa.153660 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 2.6e-213
Identity = 391/780 (50.13%), Postives = 510/780 (65.38%), Query Frame = 1

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  ++ VP  +G +GAV+VE E     + K I  I+  D+ +  + TF   SWV SK   
Sbjct: 134 YEAKMSVPASFGPVGAVLVENEHHREMFIKDIKLITGGDEST--AITFDVASWVHSKFDD 193

Query: 123 DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYND 182
            + R FF+ KSYLP +TP G+  LR ++L  LRG         +ERK  ER+YDYD YND
Sbjct: 194 PEPRAFFTVKSYLPSQTPPGIEALRKKELETLRGD------GHSERKFHERVYDYDTYND 253

Query: 183 LGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQ 242
           LGD D   + KRPVLG  +HPYPRRCRTGR     DP +E RS    YVPRDE+FS+VK 
Sbjct: 254 LGDPDKNIDHKRPVLGTKEHPYPRRCRTGRPKTLYDPETETRSSP-VYVPRDEQFSDVKG 313

Query: 243 HYFPSSE---------PDNKDLLGKD-SFSDLPHIESMFREGIKAPHAPHKLLTFNLSTI 302
             F ++          P    LL     FS  P I++++ +GI  P   H   +FN+   
Sbjct: 314 RTFSATTLRSGLHAILPAVAPLLNNSHGFSHFPAIDALYSDGIPLPVDGHGGNSFNVIND 373

Query: 303 VTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTR 362
           V P    +  ++   V    L +  PE   RDR+SW  D EFARQTLAGLNP  I+ +T 
Sbjct: 374 VIPRVVQMIEDTTEHV----LRFEVPEMLERDRFSWFRDEEFARQTLAGLNPICIRRLTE 433

Query: 363 LPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVR 422
            P++S+LDP  YGP ESA +   +++++   + V EA+E KRLF+LDYHD  +PYV++VR
Sbjct: 434 FPIVSKLDPAVYGPAESALSKEILEKMMNGRMTVEEAMEKKRLFLLDYHDVFLPYVHRVR 493

Query: 423 KINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAK 482
           ++  TTLYGSRT+FFL  + TL+PL IELTRP     PQWK+ FT G +AT+ WLW++AK
Sbjct: 494 ELPDTTLYGSRTVFFLSDEGTLMPLAIELTRPQSPTKPQWKRAFTHGSDATESWLWKLAK 553

Query: 483 AHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANAR 542
           AHVL HD+  HQLV HWLR H C+EPY IATNRQLS +HP+YRLLHPHFRY M INA AR
Sbjct: 554 AHVLTHDTGYHQLVSHWLRTHACVEPYIIATNRQLSRMHPVYRLLHPHFRYTMEINALAR 613

Query: 543 ESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDV 602
           E+LINA GIIE  F    YS+ELSS  Y   W+F+ +ALPEDLI RG+A R+  D G   
Sbjct: 614 EALINADGIIEEAFLAGKYSIELSSVAYGAAWQFNTEALPEDLINRGLAVRR--DDGE-- 673

Query: 603 LELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPD 662
           LELAIKDYP+A+DGLL+W ++ +W ++YV+ YY  D + V  D+EL+AWW E++ KGH D
Sbjct: 674 LELAIKDYPYADDGLLIWGSIKQWASDYVDFYYKSDGD-VAGDEELRAWWEEVRTKGHAD 733

Query: 663 KKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNI-LTE 722
           KK+   WP   T+ +L++I + I WV SGHHA+VNF QY YAGY PNRP++ R NI + E
Sbjct: 734 KKDEPWWPVCDTKENLVQILTIIMWVTSGHHAAVNFGQYHYAGYFPNRPTVVRRNIPVEE 793

Query: 723 DYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPS 782
           +     ++F+  PE  LLQ  PS  QA  V  T+ +LS+HSPDEEY+G+  EPAW  EP 
Sbjct: 794 NRDDEMKKFMARPEEVLLQSLPSQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWLAEPM 853

Query: 783 ISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
           +  AF++F   LK+ E  ID  N N + KNR GAG+VPY++LKP S  G+TGRG+P S+S
Sbjct: 854 VKAAFEKFSGRLKEAEGTIDMRNNNPENKNRCGAGIVPYELLKPFSEPGVTGRGIPNSIS 895

BLAST of Cucsa.153660 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 711.4 bits (1835), Expect = 1.1e-203
Identity = 391/834 (46.88%), Postives = 522/834 (62.59%), Query Frame = 1

Query: 24  NIILKFASIELDSDQ-QQKPFIKCVVEPILPDDETEEYKKYVGEVEVPEGYGEIGAVIVE 83
           ++ L+  S ELD+   ++K  ++     +  DD+      Y  + +VP G+G IGA+IV 
Sbjct: 113 SLSLELVSSELDAKTGKEKATVRSYAHNV--DDDDHSVVTYEADFDVPSGFGPIGAIIVT 172

Query: 84  LEGDTTKYEKFIDTISITDKKSRNSTT---FSCKSWVQSKSVLDQ----RRVFFSTKSYL 143
            E    + E F++ I++T      ++T     C SWVQ KSV D+    +R+FF+ K+YL
Sbjct: 173 NE---LRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVGDEGTPSKRIFFANKTYL 232

Query: 144 PEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDCDGPAEWKRP 203
           P +TP GL   R  DL   RG   D T    ER+A +R+YDYD YNDLG+ D   +  RP
Sbjct: 233 PGQTPAGLRSYRKNDLQQKRG---DGT---GEREADDRVYDYDVYNDLGNPDSNGDLARP 292

Query: 204 VLGGSDH-PYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQHYF--------- 263
           VLGG+   PYPRRCRTGR   K DP SE R K   YVPRDEEFS  K+ YF         
Sbjct: 293 VLGGNKQFPYPRRCRTGRPPSKKDPKSETR-KGNVYVPRDEEFSPEKEDYFLRKTVGSVL 352

Query: 264 PSSEPDNKDLLGKDSFSDLPH-----IESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDL 323
            ++ P  + LL      +LP      I+ +F +G++ P      L F  S +     P L
Sbjct: 353 QAAVPAAQSLLLDKLKWNLPFPSFFVIDKLFEDGVELPGVDK--LNFLESVV-----PRL 412

Query: 324 HSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELD 383
             +   +    +L +  P + ++D+++WL D EFAR+TLAG+NPY+I+LV   PL S+LD
Sbjct: 413 LEHLRDTPAEKILRFETPANIQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLD 472

Query: 384 PDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLY 443
           P  YGP ESA     ++E +   + V EAI  KRLF+LD+HD  +PYV+K+R ++ TT+Y
Sbjct: 473 PAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLDHTTMY 532

Query: 444 GSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDS 503
           GSRT+FFL  D TL  L IELTRP     PQW+Q+FTP  +AT  WLWR+AKAHV AHD+
Sbjct: 533 GSRTVFFLTDDGTLQLLAIELTRPASPSQPQWRQVFTPSTDATMSWLWRMAKAHVRAHDA 592

Query: 504 CIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGG 563
             H+L+ HWLR HC +EPY IA NRQLS +HPIY+LL PHFRY MRINA AR +LI+AGG
Sbjct: 593 GHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLRPHFRYTMRINARARSALISAGG 652

Query: 564 IIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAER-KKGDYGRDVLELAIKD 623
           IIE +FS   YS+ELSS  Y   WRFD +ALP DL+RRGMAE     ++G   L+LAI+D
Sbjct: 653 IIERSFSPQKYSMELSSVAYDKLWRFDTEALPADLVRRGMAEEDPTAEHG---LKLAIED 712

Query: 624 YPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEG--W 683
           YPFANDGLL+W+A+  WV  YV  +Y  D ++V  D+ELQA+W E++ KGH DKK+   W
Sbjct: 713 YPFANDGLLIWDAIKTWVQAYVARFY-PDADSVAGDEELQAFWTEVRTKGHGDKKDAPWW 772

Query: 684 PTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTED--YHQLP 743
           P L +   L    +TI WV + HHA+VNF QY + GY PNRPSIART +  E+       
Sbjct: 773 PKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAM 832

Query: 744 EEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFK 803
           E F+D P+  L + FPS  QA+VV   + +LS+HS DEEY+G E    W  + ++  A+ 
Sbjct: 833 ERFLDNPDQALRECFPSQVQATVVMAVLDVLSSHSTDEEYLGGEQTRPWNSDAAVQAAYD 892

Query: 804 RFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
            F A LK++E  ID  NK+ KLKNR GAG++PY ++KP S  G+TG G+P S S
Sbjct: 893 GFAARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDSGVTGMGIPNSTS 923

BLAST of Cucsa.153660 vs. Swiss-Prot
Match: LOX21_HORVU (Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.7e-199
Identity = 396/874 (45.31%), Postives = 521/874 (59.61%), Query Frame = 1

Query: 4   EVIASVTVK-PKTKGPTYPYANIILK------FASIELD--SDQQQKPFIKCVVEPILPD 63
           E+ A+VTV   K  G      ++ILK        S ELD  + Q+++P    V      D
Sbjct: 87  EMKATVTVHMSKAAGVRDFLYDLILKTWLHVDLVSSELDPQTGQEREPISGAVKHSGRVD 146

Query: 64  DETEEYKKYVGEVEVPEGYGEIGAVIVELEGDTTKY---EKFIDTISITDK-KSRNSTTF 123
           DE + Y+      +VP  +G IGAV V      T Y   E  +  I +    +  ++ TF
Sbjct: 147 DEWDMYE---ATFKVPASFGPIGAVQV------TNYHHSEMLLGDIEVFPTGQEESAVTF 206

Query: 124 SCKSWVQSKSVLDQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKA 183
            CKSW+        +RVFF   SYLP +TP G+  LR  +L  LRG          ERK 
Sbjct: 207 HCKSWIDPSHCTPDKRVFFPAHSYLPSQTPKGVEGLRKRELEILRG------TGCGERKE 266

Query: 184 FERIYDYDFYNDLGDCDGPAE-WKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRS-KER 243
            +RIYDYD YNDLG+ D       RPVLGG +HPYPRRCRTGR   K DP SE+RS KE 
Sbjct: 267 HDRIYDYDVYNDLGNPDDDNNPTTRPVLGGKEHPYPRRCRTGRPRSKKDPFSEERSHKEH 326

Query: 244 FYVPRDEEFSEVKQHYFPSSEPDNKDLLGK---------------DSFSDLPHIESMFRE 303
            YVPRDE F+E K   F     D K  + +                SF  L  I+ ++ +
Sbjct: 327 IYVPRDEAFTERKMGAF-----DTKKFMSQLHALTTGLKTAKHKSQSFPSLSAIDQLYDD 386

Query: 304 GIK-APHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSSL---LHYPPPESYRRDRYSWL 363
             +  P  P       +  ++  +   L     ++    +     +  PE + RD+++W 
Sbjct: 387 NFRNQPVQPEGGKLRFVIDLLETELLHLFKLEGAAFLEGIRRVFKFETPEIHDRDKFAWF 446

Query: 364 SDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEA 423
            D EFARQT+AG+NP SIQLVT  P+ S LD  TYGP +S      V+E I   +  +EA
Sbjct: 447 RDEEFARQTIAGMNPMSIQLVTEFPIKSNLDEATYGPADSLITKEVVEEQIRRVMTADEA 506

Query: 424 IELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGY 483
           ++ K+LF+LDYHD L+PYV+KVRK++GTTLYGSR LFFL +D TL P+ IELTRP     
Sbjct: 507 VQNKKLFMLDYHDLLLPYVHKVRKLDGTTLYGSRALFFLTADGTLRPIAIELTRPKSKKK 566

Query: 484 PQWKQIFTPGIEA--TDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQL 543
           PQW+Q+FTPG +   T  WLW++AKAH+LAHD+ +HQLV HWLR H C EPY IA NRQL
Sbjct: 567 PQWRQVFTPGCDGSVTGSWLWQLAKAHILAHDAGVHQLVSHWLRTHACTEPYIIAANRQL 626

Query: 544 STVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFD 603
           S +HP+YRLLHPHFR+ M INA AR  LINAGGIIE +F    YS+ELSS  Y  QWRFD
Sbjct: 627 SQMHPVYRLLHPHFRFTMEINAQARAMLINAGGIIEGSFVPGEYSLELSSVAYDQQWRFD 686

Query: 604 EQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGD 663
            +ALPEDLIRRGMA R         LELAI+DYP+ANDGLL+W+A+ +W   YV HYY  
Sbjct: 687 MEALPEDLIRRGMAVRNP----NGELELAIEDYPYANDGLLVWDAIKQWALTYVQHYYPC 746

Query: 664 DENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVN 723
             + +++D+ELQAWW E++ KGH DK++   WP L +  +L +  +TI WV SGHHA+VN
Sbjct: 747 AAD-IVDDEELQAWWTEVRTKGHADKQDEPWWPELDSHENLAQTLATIMWVTSGHHAAVN 806

Query: 724 FIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMIL 783
           F QY  AGY PNRP++AR N+ TE       +F++ PE  LL  FPS  Q+++V   + L
Sbjct: 807 FGQYPMAGYIPNRPTMARRNMPTEIGGDDMRDFVEAPEKVLLDTFPSQYQSAIVLAILDL 866

Query: 784 LSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGV 830
           LS HS DEEY+G   EPAW  +  I++AF+ F+ S + + +Q+D+ N +   KNRHGAG+
Sbjct: 867 LSTHSSDEEYMGTHEEPAWTKDGVINQAFEEFKESTRKIVEQVDEWNNDPDRKNRHGAGM 926

BLAST of Cucsa.153660 vs. TrEMBL
Match: S4UKZ5_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.9e-234
Identity = 438/785 (55.80%), Postives = 535/785 (68.15%), Query Frame = 1

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  + +VP  +GEIGA++VE E    + E F+  I + D     S   SC SW+ SK   
Sbjct: 179 YETDFKVPVDFGEIGAILVENEH---RKEMFLKEI-VVDGLPCGSVHHSCNSWIHSKYDN 238

Query: 123 DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYND 182
             +RVFF+ KSYLP +TP GL KLR E+L  LRG          ERK+FERIYDYD YND
Sbjct: 239 PAKRVFFTNKSYLPSQTPSGLAKLREEELVTLRGN------GQGERKSFERIYDYDVYND 298

Query: 183 LGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQ 242
           LGD D     +RPVLGG + PYPRRCRTGRL C T  + EKRS++ +YVPRDE FSEVKQ
Sbjct: 299 LGDPDKNLRLERPVLGGKEFPYPRRCRTGRLPCDTGSLYEKRSRKHWYVPRDEAFSEVKQ 358

Query: 243 HYF-------------PSSEPDNKDL-LGKDSFSDLPHIESMFREGIKAPHAPHKLLTFN 302
             F             PS E    D  LG   F     I+S+F EGI+ P        F 
Sbjct: 359 LTFSAKTLYSVMHALVPSLEIAIADTDLG---FKYFTAIDSLFHEGIQLP-------PFK 418

Query: 303 LSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQ 362
              ++    P L    +S     +L + PPE+  RD++ W  D EFARQTLAGLNPYSI+
Sbjct: 419 EQGVLKALLPRLVKVMSSG--DDVLRFVPPETMNRDKFFWFRDDEFARQTLAGLNPYSIK 478

Query: 363 LVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYV 422
           LVT  PL SELDP+ YGP ESA     +++ I     + EAIE K+LF+LDYHD  +PYV
Sbjct: 479 LVTEWPLKSELDPEIYGPPESAITKEIIEQEIRGFATITEAIEEKKLFILDYHDLFLPYV 538

Query: 423 NKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLW 482
           +KVRK+ GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQWKQ+F P   ATD+WLW
Sbjct: 539 SKVRKLEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQWKQVFQPAWNATDVWLW 598

Query: 483 RIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRIN 542
           R+AKAHVLAHDS  HQLV HWLR HC  EPY IATNRQLS +HPIYRLLHPHFRY M IN
Sbjct: 599 RLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHPIYRLLHPHFRYTMEIN 658

Query: 543 ANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGD 602
           + ARESLINAGGIIE++FS   YS+EL S VY  +WRFD++ALP DLIRRGMA E     
Sbjct: 659 SLARESLINAGGIIETSFSPKKYSLELCSVVYGKEWRFDQEALPADLIRRGMAVEDPTAP 718

Query: 603 YGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQE 662
           +G   L L I+DYPFANDGLLLW+A+ +WVT+YVNHYY  D + V  D+ELQAWW EI+ 
Sbjct: 719 HG---LRLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY-PDSSIVQTDQELQAWWTEIKT 778

Query: 663 KGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTN 722
            GH DKK+   WP L T  DL+ I +T+ WV SGHHA+VNF QYAYAGY P+RP+IARTN
Sbjct: 779 VGHADKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQYAYAGYFPSRPTIARTN 838

Query: 723 ILTEDYHQLP-EEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAW 782
           + TED  +   + F+  P++ LLQ FP+  QA+ +   + +LS HSPDEEYIG+++E AW
Sbjct: 839 MPTEDPSEEDWKNFVRKPDSALLQCFPTQIQATTIMAVLDILSNHSPDEEYIGEKMEQAW 898

Query: 783 ALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGV 830
           A EP I  AF+RF+  L  LE+ ID  N +++LKNR+GAGVVPY++LKP S  G+TG+GV
Sbjct: 899 AEEPVIKAAFERFKGRLLALERTIDDRNASSELKNRNGAGVVPYELLKPFSQPGVTGKGV 937

BLAST of Cucsa.153660 vs. TrEMBL
Match: S4UKU6_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6b PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 5.5e-234
Identity = 437/785 (55.67%), Postives = 534/785 (68.03%), Query Frame = 1

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  + +VP  +GEIGA++VE E    + E F+  I + D     S   SC SW+ SK   
Sbjct: 179 YETDFKVPVDFGEIGAILVENEH---RKEMFLKEI-VVDGLPCGSVHHSCNSWIHSKYDN 238

Query: 123 DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYND 182
             +RVFF+ KSYLP +TP GL KLR E+L  LRG          ERK+FERIYDYD YND
Sbjct: 239 PAKRVFFTNKSYLPSQTPSGLAKLREEELVTLRGN------GQGERKSFERIYDYDVYND 298

Query: 183 LGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQ 242
           LGD D     +RPVLGG + PYPRRCRTGRL C T  + EKRS++ +YVPRDE FSEVKQ
Sbjct: 299 LGDPDKNLRLERPVLGGKEFPYPRRCRTGRLPCDTGSLYEKRSRKHWYVPRDEAFSEVKQ 358

Query: 243 HYF-------------PSSEPDNKDL-LGKDSFSDLPHIESMFREGIKAPHAPHKLLTFN 302
             F             PS E    D  LG   F     I+S+F EGI+ P        F 
Sbjct: 359 LTFSAKTLYSVMHALVPSLEIAIADTDLG---FKYFTAIDSLFHEGIQLP-------PFK 418

Query: 303 LSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQ 362
              ++    P L    +S     +L + PPE+  RD++ W  D EFARQTLAGLNPYSI+
Sbjct: 419 EQGVLKALLPRLVKVMSSG--DDVLRFVPPETMNRDKFFWFRDDEFARQTLAGLNPYSIK 478

Query: 363 LVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYV 422
           LVT  PL SELDP+ YGP ESA     +++ I     + EAIE K+LF+LDYHD  +PYV
Sbjct: 479 LVTEWPLKSELDPEIYGPPESAITKEIIEQEIRGFATITEAIEEKKLFILDYHDLFLPYV 538

Query: 423 NKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLW 482
           +KVRK+ GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQWKQ+F P   ATD+WLW
Sbjct: 539 SKVRKLEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQWKQVFQPAWNATDVWLW 598

Query: 483 RIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRIN 542
           R+AKAHVLAHDS  HQLV HWLR HC  EPY IATNRQLS +HPIYRLLHPHFRY M IN
Sbjct: 599 RLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHPIYRLLHPHFRYTMEIN 658

Query: 543 ANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGD 602
           + ARESLINAGGIIE++FS   YS+EL S  Y  +WRFD++ALP DLIRRGMA E     
Sbjct: 659 SLARESLINAGGIIETSFSPKKYSLELCSVAYGKEWRFDQEALPADLIRRGMAVEDPTAP 718

Query: 603 YGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQE 662
           +G   L L I+DYPFANDGLLLW+A+ +WVT+YVNHYY  D + V  D+ELQAWW EI+ 
Sbjct: 719 HG---LRLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY-PDSSIVQTDQELQAWWTEIKT 778

Query: 663 KGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTN 722
            GH DKK+   WP L T  DL+ I +T+ WV SGHHA+VNF QYAYAGY P+RP+IARTN
Sbjct: 779 VGHADKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQYAYAGYFPSRPTIARTN 838

Query: 723 ILTEDYHQLP-EEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAW 782
           + TED  +   + F+  P++ LLQ FP+  QA+ +   + +LS HSPDEEYIG+++E AW
Sbjct: 839 MPTEDPSEEDWKNFVRKPDSALLQCFPTQIQATTIMAVLDILSNHSPDEEYIGEKMEQAW 898

Query: 783 ALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGV 830
           A EP I  AF+RF+  L  LE+ ID  N +++LKNR+GAGVVPY++LKP S  G+TG+GV
Sbjct: 899 AEEPVIKAAFERFKGRLLALERTIDDRNASSELKNRNGAGVVPYELLKPFSQPGVTGKGV 937

BLAST of Cucsa.153660 vs. TrEMBL
Match: S4UL90_MALDO (Lipoxygenase OS=Malus domestica GN=LOX6a PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.6e-233
Identity = 434/790 (54.94%), Postives = 530/790 (67.09%), Query Frame = 1

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  +++VP  +GEIGA++VE E     + K I    + D     S   SC SW+ SK   
Sbjct: 180 YETDIKVPVDFGEIGAILVENEHHKEMFLKEI----VVDGLPCGSVHHSCNSWIHSKYDN 239

Query: 123 DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYND 182
            ++RVFF+ KSYLP +TP GL++LR E+L  LRG          ERK+FERIYDYD YND
Sbjct: 240 PEKRVFFTNKSYLPSQTPSGLVRLREEELLTLRGN------GQGERKSFERIYDYDVYND 299

Query: 183 LGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQ 242
           LGD D     +RPVLGG + P+PRRCRTGR  C  DP+SEKRS++ +YVPRDE FSEVKQ
Sbjct: 300 LGDPDKNLRLQRPVLGGKEFPHPRRCRTGRPPCDIDPLSEKRSRKHWYVPRDEAFSEVKQ 359

Query: 243 HYF-------------PSSEPDNKDL-LGKDSFSDLPHIESMFREGIKAPHAPHK----- 302
             F             PS E    D  LG   F     I+S+F EGI  P    +     
Sbjct: 360 LTFSAKTLYSVMHALVPSLEMAIADTNLG---FKYFTAIDSLFHEGIHLPPFKEQGVLKA 419

Query: 303 LLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLN 362
           LL   ++ + T D               +L + PPE+  RD++ W  D EF RQTLAGLN
Sbjct: 420 LLPRLVNVMATGD--------------DVLRFVPPETMNRDKFFWFRDEEFGRQTLAGLN 479

Query: 363 PYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDT 422
           PYSI+LVT  PL SELDP  YGP ESA  +  ++  IG    + EAI  K+LF+LDYHD 
Sbjct: 480 PYSIKLVTEWPLKSELDPAIYGPPESAITNEIIEREIGGFATITEAIREKKLFILDYHDL 539

Query: 423 LMPYVNKVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEAT 482
            +PYV+KVRK+ GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQWKQ+F P   +T
Sbjct: 540 FLPYVSKVRKLEGTTLYGSRTLFFLTPEGTLRPLVIELTRPPMDGKPQWKQVFQPAWNST 599

Query: 483 DLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRY 542
           D+WLWR+AKAHVLAHDS  HQLV HWLR HC  EPY IATNRQLS +HPIYRLLHPHFRY
Sbjct: 600 DVWLWRLAKAHVLAHDSGYHQLVSHWLRTHCATEPYIIATNRQLSVMHPIYRLLHPHFRY 659

Query: 543 NMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-E 602
            M IN+ AR+SLINA GIIE++FS   YS+EL S  Y  +WRFD++ALP DLIRRGMA E
Sbjct: 660 TMEINSLARDSLINADGIIETSFSPGKYSLELCSIAYGKEWRFDQEALPADLIRRGMAVE 719

Query: 603 RKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWW 662
                +G   L L I+DYPFANDGLLLW+A+ +WVT+YVNHYY  D   V  D+ELQAWW
Sbjct: 720 DPTAPHG---LRLTIEDYPFANDGLLLWDAIKQWVTDYVNHYY-PDSILVQTDEELQAWW 779

Query: 663 NEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPS 722
            EI+  GH DKK+   WP L T  DL+ I +T+ WV SGHHA+VNF QYAY GY PNRP+
Sbjct: 780 TEIKTVGHADKKDEPWWPELNTPEDLMGIITTMVWVASGHHAAVNFGQYAYGGYFPNRPT 839

Query: 723 IARTNILTEDYHQLP-EEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDE 782
           +ARTN+ TED  +   + FI  PE+ LLQ FPS  QA+ +   + +LS HSPDEEYIG++
Sbjct: 840 VARTNVPTEDPSEEDWKNFIKKPESALLQCFPSQIQATRIMAVLDILSNHSPDEEYIGEK 899

Query: 783 IEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGI 830
           +E AWA EP I  AF+RF+  L  LE  ID  N N++LKNRHGAGV+PY++LKP S  G+
Sbjct: 900 MEQAWAEEPVIKAAFERFKGRLLALEGSIDDRNANSELKNRHGAGVLPYELLKPFSQPGV 938

BLAST of Cucsa.153660 vs. TrEMBL
Match: A0A067L4M8_JATCU (Lipoxygenase OS=Jatropha curcas GN=JCGZ_16708 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 2.6e-231
Identity = 429/823 (52.13%), Postives = 544/823 (66.10%), Query Frame = 1

Query: 24  NIILKFASIELDSDQQ-QKPFIKCVVEPILPDDETEEYKKYVGEVEVPEGYGEIGAVIVE 83
           +++L+  S ELD +   +K  +K      L  ++  +  K   +  VP G+GE+GAV++E
Sbjct: 104 SLLLELVSAELDPNTGLEKETVKSYAHIQLGTNKPGK-GKLEAKFNVPAGFGEVGAVLIE 163

Query: 84  LEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFSTKSYLPEKTPGG 143
            E      E FI++I + D       T SC SW  SK    ++R+FF+ KSYLP +TP G
Sbjct: 164 NEHHK---EVFIESI-VLDGFPNGPVTVSCNSWAHSKHDNPKKRIFFTNKSYLPAETPSG 223

Query: 144 LLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGSDH 203
           L +LR E+L NLRG          ERK+FERIYDYD YNDLGD D   +  RPVLG  ++
Sbjct: 224 LKRLREEELENLRGN------GQGERKSFERIYDYDTYNDLGDPDKDQDLARPVLGTKEY 283

Query: 204 PYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQHYF-------------PSSE 263
           PYPRRCRTGR   K DP+SE RS    YVPRDE FSE+K+  F             PS E
Sbjct: 284 PYPRRCRTGRPRTKKDPLSESRSSGDIYVPRDEAFSELKEATFGINTVKSLLHALVPSIE 343

Query: 264 PDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVP 323
               D  G   F     I+ +F EGI+ P    K   + L T++          +     
Sbjct: 344 TAIVD--GNLGFPYFTAIDKLFNEGIELPKDVEK--PWYLQTLLPRTV-----KAAKETK 403

Query: 324 SSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQES 383
             +L +  P+ + RD++SW  D EF+RQTLAGLNP+ IQLVT  PL SELDP+ YGP ES
Sbjct: 404 DDILLFETPQLFDRDKFSWFRDEEFSRQTLAGLNPFGIQLVTEWPLKSELDPEIYGPAES 463

Query: 384 AFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLK 443
           A     V++ I   + V EA++ KRLF+LDYHD L+PYV KVR++ GTTLYGSRTLFFL 
Sbjct: 464 AITKEIVEKEIRGFMTVEEAVKQKRLFMLDYHDILLPYVEKVRELEGTTLYGSRTLFFLV 523

Query: 444 SDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHW 503
           ++  L+P+ IELTRPP+   PQWKQ+FTPG +AT  WLWR+AKAHV+AHDS IHQLV HW
Sbjct: 524 NESNLMPVAIELTRPPIGDKPQWKQVFTPGHDATGCWLWRLAKAHVIAHDSGIHQLVSHW 583

Query: 504 LRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTA 563
           LR H C+EPY IA NRQLS +HPIYR LHPHFRY M INA AR+ LINA GIIESTFS  
Sbjct: 584 LRTHACVEPYIIAANRQLSEMHPIYRFLHPHFRYTMEINALARDKLINADGIIESTFSPG 643

Query: 564 SYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFANDGLLL 623
            YS+ELSS  Y   WRFD +ALP DLI+RGMAE  +    +  L+LAI DYPFANDGL+L
Sbjct: 644 KYSIELSSVAYDKLWRFDTEALPADLIKRGMAE--EDPTAKHGLKLAIDDYPFANDGLML 703

Query: 624 WNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLI 683
           W+A+ +W T+YVNHYY  + + V +D ELQAWW E++ KGH DKK+   WP LKT+ DLI
Sbjct: 704 WDAIKQWATDYVNHYY-PEASKVASDTELQAWWEEVRTKGHADKKDEPWWPVLKTQEDLI 763

Query: 684 KIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLP-EEFIDLPENEL 743
           ++ +TI WV SGHHA+VNF QY +AGY PNRP++ARTN+ TE+  +   E F+  PE  L
Sbjct: 764 QVLTTITWVTSGHHAAVNFGQYVFAGYFPNRPTVARTNMPTEEPSEEDFELFLKKPEAAL 823

Query: 744 LQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQ 803
           LQ FPS  QA+ V   + +LS+HSPDEEYIG  +EP+W   P+I  A++RF A LK+LE 
Sbjct: 824 LQCFPSQQQATKVMAILDVLSSHSPDEEYIGHTLEPSWEANPAIKSAYERFNARLKELEA 883

Query: 804 QIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
            ID+ N + K  NR GAGVVPY++LKP S  G+TG+GVP S+S
Sbjct: 884 TIDERNNDLKNSNRAGAGVVPYELLKPVSESGVTGKGVPNSIS 903

BLAST of Cucsa.153660 vs. TrEMBL
Match: M5W1M2_PRUPE (Lipoxygenase OS=Prunus persica GN=PRUPE_ppa000968mg PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 1.4e-229
Identity = 434/784 (55.36%), Postives = 526/784 (67.09%), Query Frame = 1

Query: 63  YVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVL 122
           Y  + +VP  +GE+GAV+VE E     + K I    + D     S   SC SWV SK   
Sbjct: 189 YETDFKVPVHFGEVGAVLVENEHHKEMFLKDI----VLDGLPYGSVHLSCNSWVHSKYDN 248

Query: 123 DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYND 182
            ++RVFF+ KSYLP +TP GL++LR E+L  LRG          ERK FERIYDYD YND
Sbjct: 249 PEKRVFFTNKSYLPSQTPNGLVRLREEELVTLRGN------GQGERKFFERIYDYDVYND 308

Query: 183 LGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQ 242
           LG+ D     +RPVLGG + PYPRRCRTGR  C+TD +SEKRS  ++YVPRDE FSEVKQ
Sbjct: 309 LGEPDKNLRLERPVLGGIEFPYPRRCRTGRPQCETDSLSEKRSN-KWYVPRDEAFSEVKQ 368

Query: 243 HYF-------------PSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNL 302
             F             PS E    D      F+    I+S+F EGI  P        F  
Sbjct: 369 LTFSAKTVYSVMHALVPSLETAMAD--NDHGFAYFTAIDSLFNEGINLP-------PFKE 428

Query: 303 STIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQL 362
             I+    P L +   S     +L + PPE+  RD++ W  D EFARQTLAGLNP S++L
Sbjct: 429 QGILKTLLPRLVNVVASG--DDVLRFVPPETMNRDKFFWFRDEEFARQTLAGLNPCSLKL 488

Query: 363 VTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVN 422
           VT+ PL SELDP+ YGP ESA     +++ I     V EAI  K+LF+LDYHD  +PYV+
Sbjct: 489 VTKWPLKSELDPEIYGPPESAITKEIIEQEIRGFPTVQEAIREKKLFILDYHDLFLPYVS 548

Query: 423 KVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWR 482
           KVRKI GTTLYGSRTLFFL  + TL PL IELTRPP+DG PQWKQ+F P   AT +WLWR
Sbjct: 549 KVRKIEGTTLYGSRTLFFLTREGTLRPLAIELTRPPMDGKPQWKQVFQPSWNATGVWLWR 608

Query: 483 IAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINA 542
           +AKAHVLAHDS  HQLV HWLR HC  EPY IAT+RQLS +HPIYR LHPHFRY M INA
Sbjct: 609 LAKAHVLAHDSGYHQLVSHWLRTHCVTEPYIIATSRQLSVMHPIYRFLHPHFRYTMEINA 668

Query: 543 NARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGDY 602
            ARESLINAGGIIES+FS   YS+ELS+  Y  +WRFD++ALP DLIRRGMA E     +
Sbjct: 669 LARESLINAGGIIESSFSPGKYSLELSAVAYGKEWRFDQEALPADLIRRGMAVEDPTAPH 728

Query: 603 GRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEK 662
           G   L+L I+DYPFANDGLL+W+A+ +WVT+YVNHYY  D + V  D ELQAWW EI+  
Sbjct: 729 G---LKLTIEDYPFANDGLLMWDAIKQWVTDYVNHYY-PDSSLVQTDGELQAWWTEIKTV 788

Query: 663 GHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNI 722
           GH DKK+   WP L T  DLI I +T+ WV SGHHA+VNF QY YAGY PNRP+IARTN+
Sbjct: 789 GHADKKDEPWWPELNTPEDLIGIITTMVWVTSGHHAAVNFGQYVYAGYFPNRPTIARTNM 848

Query: 723 LTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWA 782
            TED   +  + F+  PE  LL+ FPS  QA+ +   + +LS HSPDEEYIG+++E AWA
Sbjct: 849 PTEDPSPEFWKSFLKKPEVALLRCFPSQIQATRIMAVLDILSNHSPDEEYIGEKMEAAWA 908

Query: 783 LEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVP 830
            EP I  AF+RF+  L  +E  ID  N N++LKNR+GAGVVPY++LKP S  G+TG GVP
Sbjct: 909 EEPVIKAAFERFKGRLLVIEGIIDDRNANSELKNRNGAGVVPYELLKPFSQPGVTGMGVP 946

BLAST of Cucsa.153660 vs. TAIR10
Match: AT3G45140.1 (AT3G45140.1 lipoxygenase 2)

HSP 1 Score: 785.8 bits (2028), Expect = 2.6e-227
Identity = 408/784 (52.04%), Postives = 520/784 (66.33%), Query Frame = 1

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           +KY  E E+PE +G +GA+ ++      +Y + +    +  K    S TF+C+SWV  KS
Sbjct: 135 EKYECEFEMPEDFGPVGAIKIQ-----NQYHRQLFLKGVELKLPGGSITFTCESWVAPKS 194

Query: 121 VLDQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFY 180
           V   +R+FFS KSYLP +TP  L K R E+L  L+G   +E   + E   FERIYDYD Y
Sbjct: 195 VDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREE---VGEFTKFERIYDYDVY 254

Query: 181 NDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEV 240
           ND+GD D   E  RPV+GG  HPYPRRC+TGR  C+TDP SE+R    FYVPRDEEFS  
Sbjct: 255 NDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTA 314

Query: 241 KQHYF---------PSSEPDNKDLL--GKDSFSDLPHIESMFREGIKAPHAPHKLLTFNL 300
           K   F         PS  P  + +L   ++ F     I+++F EGI+ P     L     
Sbjct: 315 KGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKDAGLL----- 374

Query: 301 STIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQL 360
                P  P +   +       +L +  P    RDR+SWL D EFARQTLAGLNPYSIQL
Sbjct: 375 -----PLLPRI-IKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQL 434

Query: 361 VTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVN 420
           V   PL+S+LDP  YG   S      V+  +  ++ V+EA++ KRLFVLDYHD L+PYVN
Sbjct: 435 VEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVN 494

Query: 421 KVRKINGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWR 480
           KVR++N TTLY SRTLFFL  D TL P+ IELT PP    PQWKQ+FTPG +AT  WLW 
Sbjct: 495 KVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATSCWLWN 554

Query: 481 IAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINA 540
           +AK H ++HD+  HQL+ HWLR H C EPY IA NRQLS +HPIYRLLHPHFRY M INA
Sbjct: 555 LAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINA 614

Query: 541 NARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAER-KKGDY 600
            AR+SL+N GGIIE+ F    Y++ELSS+VY   WRFD++ LP DLI+RG+AE  K  ++
Sbjct: 615 RARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEH 674

Query: 601 GRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEK 660
           G   + L I DYPFANDGL+LW+A+ EWVT+YV HYY D+E  + +D+ELQ WW+E++  
Sbjct: 675 G---VRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEE-LITSDEELQGWWSEVRNI 734

Query: 661 GHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNI 720
           GH DKK+   WP LKT++DLI + +TIAWV SGHHA+VNF QY Y GY PNRP+  R  +
Sbjct: 735 GHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRM 794

Query: 721 LTED-YHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWA 780
            TED   +  +EF + PE  LL+ +PS  QA++V  T+ LLS HSPDEEYIG++ E +WA
Sbjct: 795 PTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWA 854

Query: 781 LEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVP 830
            EP I+ AF+RF+  L+ LE  ID+ N N  LKNR GAGVV Y++LKPTS +G+TG GVP
Sbjct: 855 NEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGVTGMGVP 895

BLAST of Cucsa.153660 vs. TAIR10
Match: AT1G67560.1 (AT1G67560.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 609.8 bits (1571), Expect = 2.6e-174
Identity = 345/801 (43.07%), Postives = 473/801 (59.05%), Query Frame = 1

Query: 60  YKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSK 119
           Y  +  +  VP  +G+ GA++V    +    E  +  I I D  S ++  F   +W+ SK
Sbjct: 151 YLVFTADFTVPINFGKPGAILVT---NLLSTEICLSEIIIED--STDTILFPANTWIHSK 210

Query: 120 SVLDQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDF 179
           +   Q R+ F ++  LP +TP G+ +LR +DL ++RG          ERK  ERIYDYD 
Sbjct: 211 NDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGD------GKGERKPHERIYDYDV 270

Query: 180 YNDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKER--FYVPRDEEF 239
           YNDLGD     E  RPVLG  + PYPRRCRTGR     DP  E R KE+  FYVPRDE F
Sbjct: 271 YNDLGD-PRKTERVRPVLGVPETPYPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVF 330

Query: 240 SEVKQHYF-------------PS--SEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPH 299
            E+K+  F             PS  +   N D+     F+    I+++++  I   H   
Sbjct: 331 EEIKRDTFRAGRFKALFHNLVPSIAAALSNLDI----PFTCFSDIDNLYKSNIVLGHTEP 390

Query: 300 KLLTFNLSTIVTPDKPDLHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGL 359
           K     L   +         N   +V  +LL Y  P   + DR++WL D EF RQ LAG+
Sbjct: 391 K--DTGLGGFIGG-----FMNGILNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQALAGV 450

Query: 360 NPYSIQLVTRLPLMSELDPDTYGPQESAFNHTKVQELIGCSIE-----VNEAIELKRLFV 419
           NP +I+L+  LP+ S LDP  YGPQES       +E+I   +E     + +A+E KRLF+
Sbjct: 451 NPVNIELLKELPIRSNLDPALYGPQESVLT----EEIIAREVEHYGTTIEKALEEKRLFL 510

Query: 420 LDYHDTLMPYVNKVRKI--NGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQI 479
           +DYHD L+P+V K+  I  +    Y SRT+FF   +  L PL IEL+ PP     + K +
Sbjct: 511 VDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELSLPPT-AESENKFV 570

Query: 480 FTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYR 539
           +T G +AT  W+W++AKAHV ++D+ +HQLV HWLR H  MEPY IATNRQLST+HP+Y+
Sbjct: 571 YTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIATNRQLSTMHPVYK 630

Query: 540 LLHPHFRYNMRINANARESLINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDL 599
           LLHPH RY + INA AR+SLIN GGIIES F+   Y++ELSS+ Y+  WRFD + LP DL
Sbjct: 631 LLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRFDMEGLPADL 690

Query: 600 IRRGMAERKKGDYGRDVLELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVIND 659
           +RRGMAE          + L I DYP+A DGLL+W A+ + V  YV H+Y D + ++ +D
Sbjct: 691 VRRGMAEEDSS--AECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHFYSDSK-SITSD 750

Query: 660 KELQAWWNEIQEKGHPDKKE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAG 719
            ELQAWW+EI+ KGH DKK+   WP L T  DL +I + + W+ SG HA++NF QY + G
Sbjct: 751 LELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFGQYPFGG 810

Query: 720 YTPNRPSIARTNILTE---DYHQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHS 779
           Y PNRP++ R  I  E   DY    E F+  P+   L   P+  QA+ V      LS HS
Sbjct: 811 YVPNRPTLLRKLIPQETDPDY----EMFMRNPQYSFLGSLPTQLQATKVMAVQETLSTHS 870

Query: 780 PDEEYIGD--EIEPAWALEPSISKAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPY 830
           PDEEY+ +  E++  W  +  + K F +F   L  +E+ I++ NK+ KLKNR GAG+ PY
Sbjct: 871 PDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRTGAGMPPY 916

BLAST of Cucsa.153660 vs. TAIR10
Match: AT1G72520.1 (AT1G72520.1 PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 601.7 bits (1550), Expect = 7.0e-172
Identity = 338/829 (40.77%), Postives = 487/829 (58.75%), Query Frame = 1

Query: 24  NIILKFASIELDSDQQQKPFIKCVV--EPILPDDETEEYKKYVGEVEVPEGYGEIGAVIV 83
           N++L+  S ++D    +    K  V  +     +   E   Y  E  V   +G  GA+ V
Sbjct: 124 NVVLELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAEFTVDSAFGSPGAITV 183

Query: 84  ELEGDTTKYEK--FIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFSTKSYLPEKT 143
                T K++K  F+++I+I +  +     F C SWVQS+     +R+ F+ + YLP +T
Sbjct: 184 -----TNKHQKEFFLESITI-EGFACGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSET 243

Query: 144 PGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGG 203
           P GL  LR ++L NLRG          ERK  +RIYDYD YND+G+ D   E  RP LGG
Sbjct: 244 PSGLRTLREKELENLRGN------GKGERKLSDRIYDYDVYNDIGNPDISRELARPTLGG 303

Query: 204 SDHPYPRRCRTGRLHCKTDPVSEKRSKERF--YVPRDEEFSEVKQHYFPSSE-------- 263
            + PYPRRCRTGR    TD +SE+R ++    YVPRDE+F E KQ+ F +          
Sbjct: 304 REFPYPRRCRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACRLKAVLHNL 363

Query: 264 -PDNKDLLGKDSFSDLPHIESMFREGI--KAPHAPHKLLTFNLSTIVTPDKPDLHSNSNS 323
            P  K  +  + F++   I+S+++EG+  K          F L  IVT     L  +S  
Sbjct: 364 IPSLKASILAEDFANFGEIDSLYKEGLLLKLGFQDDMFKKFPLPKIVTT----LQKSSEG 423

Query: 324 SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGP 383
                LL Y  P+   +D+Y+WL D EFARQ +AG+NP +I+ VT  P +S LDP+ YGP
Sbjct: 424 -----LLRYDTPKIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGP 483

Query: 384 Q-ESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTL 443
              SA     +   +   + V +A+E  RLF++DYHD  +P+++++  ++G   Y +RT+
Sbjct: 484 GLHSALTEDHIIGQLD-GLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTI 543

Query: 444 FFLKSDDTLIPLGIELTRPPVDGYPQW-KQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQ 503
            FL    TL P+ IEL+ P      Q  K++ TP ++AT  W+W++AKAHV ++D+ +HQ
Sbjct: 544 LFLTRLGTLKPIAIELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQ 603

Query: 504 LVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIES 563
           LV HWLR H C+EP+ +A +RQLS +HPI++LL PH RY + INA AR++LI+A G+IES
Sbjct: 604 LVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIES 663

Query: 564 TFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFA 623
            F+   Y +E+SS+ Y+++WRFD + LP DLIRRGMA       +G   L+L ++DYP+A
Sbjct: 664 CFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGMAVPDPTQPHG---LKLLVEDYPYA 723

Query: 624 NDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKK--EGWPTLK 683
           NDGLLLW+A+  WV  YV  YY  + N +  D ELQAW++E    GH D +  E WP L 
Sbjct: 724 NDGLLLWSAIQTWVRTYVERYYA-NSNLIQTDTELQAWYSESINVGHADHRDAEWWPKLS 783

Query: 684 TRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDL 743
           T  DL+ + +TI W+ S  HA++NF QY Y GY PNRP + R  I  E   +    FI+ 
Sbjct: 784 TVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIPDESDPEF-TSFIED 843

Query: 744 PENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPA-WALEPSISKAFKRFQAS 803
           P+       PS+ Q +     +  LS HSPDEEYIG+  +P+ W  +  I  AF  F A 
Sbjct: 844 PQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAE 903

Query: 804 LKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
           +  +E++IDK N++   +NR GAGV+PY+++ P+S  G+T RGVP SVS
Sbjct: 904 IGRIEKEIDKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVS 925

BLAST of Cucsa.153660 vs. TAIR10
Match: AT1G17420.1 (AT1G17420.1 lipoxygenase 3)

HSP 1 Score: 594.0 bits (1530), Expect = 1.5e-169
Identity = 333/826 (40.31%), Postives = 485/826 (58.72%), Query Frame = 1

Query: 24  NIILKFASIELDSDQQ-QKPFIKCVVEPILPDDETE-EYKKYVGEVEVPEGYGEIGAVIV 83
           NI+L+  S +LD   +  K     V++      +T+ E   Y  E  V   +G  GA+ V
Sbjct: 118 NIVLELISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPGAITV 177

Query: 84  ELEGDTTKYEK--FIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFSTKSYLPEKT 143
                  K++K  F+++I+I +  +     F C SWVQS+     +R+FF+ + YLP +T
Sbjct: 178 -----MNKHQKEFFLESITI-EGFALGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNET 237

Query: 144 PGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGG 203
           P GL  LR ++L NLRG  +        RK  +RIYD+D YNDLG+ D  +E  RP LGG
Sbjct: 238 PSGLRVLREKELKNLRGDGS------GVRKLSDRIYDFDVYNDLGNPDKSSELSRPKLGG 297

Query: 204 SDHPYPRRCRTGRLHCKTDPVSEKRSKERF--YVPRDEEFSEVKQHYFPSSE-------- 263
            + PYPRRCRTGR    +D  +E R ++    YVPRDE+F E KQ  F +          
Sbjct: 298 KEVPYPRRCRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAGRLKAVLHHL 357

Query: 264 -PDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSV 323
            P  K  +  + F+D   I+ +++EG+         L F          P +  ++    
Sbjct: 358 IPSLKASIVAEDFADFGEIDRLYKEGLLLK------LGFQDDIFKKFPLPKVVVDTLQES 417

Query: 324 PSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQE 383
              LL Y  P+   +D+ +WL D EFARQ +AG+NP +I+ V   P +S LDP  YGPQ 
Sbjct: 418 TKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQH 477

Query: 384 SAFNHTKV-QELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFF 443
           SA     +   L G S++  +A+E  RL++LDYHD  +P+++++  ++G   Y +RT+FF
Sbjct: 478 SALTDDHIIGHLDGFSVQ--QALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFF 537

Query: 444 LKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVI 503
           L    TL P+ IEL+ PP     + K++ TP ++AT  W+W++AKAHV ++D+ +HQLV 
Sbjct: 538 LTRLGTLKPVAIELSLPPHGPKHRSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVN 597

Query: 504 HWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFS 563
           HWLR H C+EP+ +A +RQLS +HPI++LL PH RY + INA AR+SLI+A G+IE  F+
Sbjct: 598 HWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISADGVIEGGFT 657

Query: 564 TASYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDG 623
             +Y +E+S++ Y+  WRFD + LP DLIRRGMA       +G   L+L I+DYP+ANDG
Sbjct: 658 AGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGMAIPDATQPHG---LKLLIEDYPYANDG 717

Query: 624 LLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEG--WPTLKTRN 683
           LLLW+A+  WV  YV  YY  + N +  D ELQ+W++E    GH D ++   WP L T +
Sbjct: 718 LLLWSAIQTWVRTYVERYY-PNPNLIKTDSELQSWYSESINVGHADLRDADWWPELSTVD 777

Query: 684 DLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPEN 743
           DL+ I +T+ W+ S  HA++NF QY Y GY PNRP + R  ++ ++       FI  PE 
Sbjct: 778 DLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMR-RLIPDESDPEYASFISHPEK 837

Query: 744 ELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPA-WALEPSISKAFKRFQASLKD 803
                 PS+ Q S     +  LS HSPDEEYIG+  +P+ W  +  I +AF  F A +  
Sbjct: 838 YYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGR 897

Query: 804 LEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
           +E++I+K N +   +NR GAGV+PY++L P+S  G+T RGVP SVS
Sbjct: 898 IEKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGVPNSVS 918

BLAST of Cucsa.153660 vs. TAIR10
Match: AT1G55020.1 (AT1G55020.1 lipoxygenase 1)

HSP 1 Score: 535.4 bits (1378), Expect = 6.2e-152
Identity = 313/763 (41.02%), Postives = 443/763 (58.06%), Query Frame = 1

Query: 91  EKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRVFFSTKSYLPEKTPGGLLKLRAED 150
           E  + ++++ D        + C SW+         RVFFS K+YLP +TP  LLK R E+
Sbjct: 122 EFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTDRVFFSNKTYLPHETPATLLKYREEE 181

Query: 151 LSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDCDGPAEWKRPVLGGS-DHPYPRRCR 210
           L +LRG          E K ++R+YDY +YNDLG    P +  RPVLGG+ ++PYPRR R
Sbjct: 182 LVSLRG------TGEGELKEWDRVYDYAYYNDLGV---PPKNPRPVLGGTQEYPYPRRGR 241

Query: 211 TGRLHCKTDPVSEKR----SKERFYVPRDEEFSEVKQHYF-------------PSSEPDN 270
           TGR   K DP +E R    S    YVPRDE F  +K   F             P+ E   
Sbjct: 242 TGRKPTKEDPQTESRLPITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVF 301

Query: 271 KDLLGK-DSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDLHSNSNSSVPSS 330
            D   + DSF D+  I   + EGI  P+         + +IV     ++      +    
Sbjct: 302 DDTPKEFDSFEDVLKI---YEEGIDLPNQAL------IDSIVKNIPLEMLKEIFRTDGQK 361

Query: 331 LLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTYGPQESAF 390
            L +P P+  + D+ +W +D EFAR+ LAGLNP  IQL+   P  S+LD ++YG Q S  
Sbjct: 362 FLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTI 421

Query: 391 NHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRTLFFLKSD 450
             + ++  +   + V EA+E +RLF+LD+HDTLMPY+ +V     T  Y SRTL FLK D
Sbjct: 422 TKSHIEHNLD-GLTVEEALEKERLFILDHHDTLMPYLGRVNTTT-TKTYASRTLLFLKDD 481

Query: 451 DTLIPLGIELTRPPVDG--YPQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIHQLVIHW 510
            TL PL IEL+ P  +G  +    +++TPG    D  LW++AKA V  +DS  HQL+ HW
Sbjct: 482 GTLKPLVIELSLPHPNGDKFGAVSEVYTPGEGVYDS-LWQLAKAFVGVNDSGNHQLISHW 541

Query: 511 LRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIESTFSTA 570
           ++ H  +EP+ IATNRQLS +HP+++LL PHFR  M INA AR+ LIN GGI E T   +
Sbjct: 542 MQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPS 601

Query: 571 SYSVELSSSVYRDQWRFDEQALPEDLIRRGMA-ERKKGDYGRDVLELAIKDYPFANDGLL 630
            Y++E+SS +Y++ W F +QALP +L +RGMA E  +  +G   L L IKDYP+A DGL 
Sbjct: 602 KYAMEMSSFIYKNHWTFPDQALPAELKKRGMAVEDPEAPHG---LRLRIKDYPYAVDGLE 661

Query: 631 LWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKE--GWPTLKTRNDL 690
           +W A+  WV +Y+  +Y  +E+ +  D ELQAWW E++E+GH DKK    WP ++TR +L
Sbjct: 662 VWYAIESWVRDYIFLFYKIEED-IQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREEL 721

Query: 691 IKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFIDLPENEL 750
           ++  + I WV S  HA+VNF QY  AGY PNRP+I+R  +  E+  +  EE    P+   
Sbjct: 722 VESCTIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKENTPEF-EELEKNPDKVF 781

Query: 751 LQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLKDLEQ 810
           L+   +  Q  +  + + +LS HS DE Y+G      WA E    +AF++F   +K++E+
Sbjct: 782 LKTITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEK 841

Query: 811 QIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
            ID+ N +  LKNR G   +PY +L P+S  G+TGRG+P SVS
Sbjct: 842 NIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVS 858

BLAST of Cucsa.153660 vs. NCBI nr
Match: gi|449448968|ref|XP_004142237.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1703.7 bits (4411), Expect = 0.0e+00
Identity = 827/830 (99.64%), Postives = 830/830 (100.00%), Query Frame = 1

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY
Sbjct: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS
Sbjct: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120

Query: 121 VLDQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFY 180
           VLDQRRVFFSTKSYLPE+TPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFY
Sbjct: 121 VLDQRRVFFSTKSYLPERTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFY 180

Query: 181 NDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEV 240
           NDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEV
Sbjct: 181 NDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEV 240

Query: 241 KQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDL 300
           KQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDL
Sbjct: 241 KQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPDKPDL 300

Query: 301 HSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELD 360
           HSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELD
Sbjct: 301 HSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELD 360

Query: 361 PDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLY 420
           PDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLY
Sbjct: 361 PDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLY 420

Query: 421 GSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDS 480
           GSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDS
Sbjct: 421 GSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHDS 480

Query: 481 CIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGG 540
           CIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGG
Sbjct: 481 CIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGG 540

Query: 541 IIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDY 600
           IIESTFSTASYSVELSSS+YRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDY
Sbjct: 541 IIESTFSTASYSVELSSSMYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDY 600

Query: 601 PFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPTL 660
           PFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPTL
Sbjct: 601 PFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPTL 660

Query: 661 KTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFID 720
           KTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFID
Sbjct: 661 KTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFID 720

Query: 721 LPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQAS 780
           LPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQAS
Sbjct: 721 LPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQAS 780

Query: 781 LKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVST 831
           LKDLEQQID+NNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVST
Sbjct: 781 LKDLEQQIDENNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVST 830

BLAST of Cucsa.153660 vs. NCBI nr
Match: gi|659097677|ref|XP_008449754.1| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1584.3 bits (4101), Expect = 0.0e+00
Identity = 770/831 (92.66%), Postives = 793/831 (95.43%), Query Frame = 1

Query: 1   MGVEVIASVTVKPKTKGPTYPYANIILKFASIELDSDQQQKPFIKCVVEPILPDDETEEY 60
           MGVE   SVTVKPKT GPTYPYANIILKFASIELDSDQQQK FIKCVVEPI PDD+TEEY
Sbjct: 1   MGVEATVSVTVKPKTTGPTYPYANIILKFASIELDSDQQQKQFIKCVVEPIRPDDDTEEY 60

Query: 61  KKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKS 120
           K+Y GEVEVPEGYGEIGAVIVELE DT KYEKFIDTIS+TDKKSR+STTFSCKSWVQSKS
Sbjct: 61  KEYEGEVEVPEGYGEIGAVIVELECDTAKYEKFIDTISLTDKKSRHSTTFSCKSWVQSKS 120

Query: 121 VLDQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFY 180
           VLDQRR+FFSTKSYLP KTPGGLLKLRAEDLSNLRGIK D TVDMNERKAFERIYDYDFY
Sbjct: 121 VLDQRRIFFSTKSYLPGKTPGGLLKLRAEDLSNLRGIKPDGTVDMNERKAFERIYDYDFY 180

Query: 181 NDLGDCDGPAEWKRPVLGGSDHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEV 240
           NDLGDCDGPAEWKRPVLGGS+ PYPRRCRTGRLHC  DPVSEKRSKERFYVPRDEEFSEV
Sbjct: 181 NDLGDCDGPAEWKRPVLGGSERPYPRRCRTGRLHCIADPVSEKRSKERFYVPRDEEFSEV 240

Query: 241 KQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFNLSTIVTPD-KPD 300
           KQHYFPSSEPDNKDLLGK+SF DLPHIESMFREGIKAP APHKLL FNLSTIVTPD KPD
Sbjct: 241 KQHYFPSSEPDNKDLLGKNSFHDLPHIESMFREGIKAPDAPHKLLKFNLSTIVTPDHKPD 300

Query: 301 LHSNSNSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSEL 360
           LHSNS  SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSEL
Sbjct: 301 LHSNS--SVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSEL 360

Query: 361 DPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTL 420
           DP+ YGPQESAFNHTKVQEL+GC  EVNEAIE KRLFV+DYHDTLMPYVNKVR INGTTL
Sbjct: 361 DPEIYGPQESAFNHTKVQELLGCFNEVNEAIECKRLFVVDYHDTLMPYVNKVRTINGTTL 420

Query: 421 YGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAHVLAHD 480
           YGSRTLFFLKSD TLIPLGIELTRPPVDGYPQWKQIFTPG EATD+WLWRIAKAHVLAHD
Sbjct: 421 YGSRTLFFLKSDGTLIPLGIELTRPPVDGYPQWKQIFTPGTEATDVWLWRIAKAHVLAHD 480

Query: 481 SCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAG 540
           SCIHQLVIHWLRAHCCMEPYAIATNRQLST+HPIYRLLHPHFRYNMRINANARESLINAG
Sbjct: 481 SCIHQLVIHWLRAHCCMEPYAIATNRQLSTLHPIYRLLHPHFRYNMRINANARESLINAG 540

Query: 541 GIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKD 600
           GIIE TFSTASYS+ELSSSVYRDQWRFDEQA PEDLIRRGMAERKK +YGRD+LEL IKD
Sbjct: 541 GIIEGTFSTASYSMELSSSVYRDQWRFDEQAFPEDLIRRGMAERKKDEYGRDILELTIKD 600

Query: 601 YPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPT 660
           YPFANDGL+LWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPT
Sbjct: 601 YPFANDGLILWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPT 660

Query: 661 LKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFI 720
           LKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFI
Sbjct: 661 LKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDYHQLPEEFI 720

Query: 721 DLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQA 780
           DLPEN LLQVFPSVDQA++VTTTMILLSAHSPDEEYIG E+EPAWALEP+I KAFKRFQA
Sbjct: 721 DLPENVLLQVFPSVDQATMVTTTMILLSAHSPDEEYIGVEVEPAWALEPAIDKAFKRFQA 780

Query: 781 SLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVST 831
           +LKDLEQQID+NNKN  LKNRHGAGVVPY+VLKPTST GITGRGVPYSVST
Sbjct: 781 NLKDLEQQIDENNKNKSLKNRHGAGVVPYEVLKPTSTDGITGRGVPYSVST 829

BLAST of Cucsa.153660 vs. NCBI nr
Match: gi|778693100|ref|XP_011653578.1| (PREDICTED: LOW QUALITY PROTEIN: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 568/827 (68.68%), Postives = 672/827 (81.26%), Query Frame = 1

Query: 9   VTVKPKTKGPTYPYANII-LKFASIELDSDQQQKPFIKCVVEPILPDDETEEYKKYVGEV 68
           VTV PK K   +P+  II LKFAS+ELDS++Q K FIKC  + +   DE+ EYKKY G++
Sbjct: 9   VTVIPK-KNEQFPWVEIIYLKFASVELDSNEQPKEFIKCEAQ-LQEVDESVEYKKYKGDI 68

Query: 69  EVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRV 128
           +V EG+G+IGAVIVEL+      E+F+DTIS+  +K   S TFSCKSWVQ K ++D RR+
Sbjct: 69  KVAEGFGKIGAVIVELQDGVN--ERFVDTISVEAEKPPISVTFSCKSWVQPKGLIDHRRI 128

Query: 129 FFST-KSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDC 188
           FFS+ KSYL  KTPGGL+KLR EDL+NLRG KAD +VD NERKAFERIYDYD YNDLGD 
Sbjct: 129 FFSSNKSYLSGKTPGGLVKLREEDLANLRGEKADGSVDRNERKAFERIYDYDLYNDLGDP 188

Query: 189 DGPAEWKRPVLGGSD-HPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQHYF 248
           D     KRPVLGGS+ +PYPRRCRTGR   + DP SEKR +E FYVPRDEEFSE+KQ   
Sbjct: 189 DQSMNLKRPVLGGSEEYPYPRRCRTGRPLTQNDPASEKRIEEWFYVPRDEEFSEIKQS-- 248

Query: 249 PSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL-LTFNLSTIVTPDKPDLHSNS 308
            SS+P N+ LL K  FSDLP +E      IK P A  K  L FN+++I++  +P    +S
Sbjct: 249 -SSQPGNEKLLRKSPFSDLPELE------IKTPSAASKFNLQFNIASILSSHRPPALKSS 308

Query: 309 NSSVPSSLLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDTY 368
           ++   S+L+  PPPESY+RD+Y+WLSD EFAR TL GLNPYSIQLV  LP MS+LD   Y
Sbjct: 309 DTP-SSTLVELPPPESYKRDKYNWLSDIEFARLTLVGLNPYSIQLVKSLPFMSKLDEGDY 368

Query: 369 GPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSRT 428
            P+ES F    VQEL+GC I V++A+  KRLFV+DYHDTLMPYV+KVRKI GTTLYGSRT
Sbjct: 369 RPRESKFTPEXVQELLGCYITVDKALADKRLFVVDYHDTLMPYVSKVRKIKGTTLYGSRT 428

Query: 429 LFFLKSDDTLIPLGIELTRPPVDGY-PQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCIH 488
           LFFL SD  L+PLGIELTRPP+DG+  QWK++FTPG  +TD+WLWR+AKAHVL+HDSCIH
Sbjct: 429 LFFLNSDGILMPLGIELTRPPMDGHLEQWKEVFTPGTNSTDVWLWRLAKAHVLSHDSCIH 488

Query: 489 QLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGIIE 548
           QLVIHWLRAHCCME YAIATNRQLST+HPIYRLLHPHFRYNMRINANAR++LINA GIIE
Sbjct: 489 QLVIHWLRAHCCMESYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINARGIIE 548

Query: 549 STFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPFA 608
           + FS ASYSVELSSS Y+  WRFDEQALPEDLI RGMAERK+   GRDVLEL IKDYPFA
Sbjct: 549 NAFSGASYSVELSSSAYK-AWRFDEQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPFA 608

Query: 609 NDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPTLKTR 668
           NDGLLLWNALLEWVTEYVNHYYGD+ENAV NDKELQAWW EIQEKGHPDK+EGWP L+TR
Sbjct: 609 NDGLLLWNALLEWVTEYVNHYYGDEENAVTNDKELQAWWGEIQEKGHPDKREGWPALRTR 668

Query: 669 NDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDY-HQLPEEFIDLP 728
           +DLIKIASTIAWVGSGHHASVNF+QYAY GY PNRP+IARTN+LTE++  ++ ++F + P
Sbjct: 669 DDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDEILKDFRNYP 728

Query: 729 ENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASLK 788
           EN+L ++FPSV QA++V  TM LLS HSPDEEYIGD IEPAWAL+ SISKAF++F+A+L 
Sbjct: 729 ENKLNELFPSVVQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDQSISKAFEKFKANLT 788

Query: 789 DLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
           DLE++ID+ N+N  LKNR+GAG++PY+V+KP S  G+TG GVPYSVS
Sbjct: 789 DLEKKIDELNENKDLKNRYGAGIIPYEVMKPRSKPGVTGIGVPYSVS 820

BLAST of Cucsa.153660 vs. NCBI nr
Match: gi|778693093|ref|XP_004142238.2| (PREDICTED: lipoxygenase 2, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 570/828 (68.84%), Postives = 667/828 (80.56%), Query Frame = 1

Query: 9   VTVKPKTKGPTYPYANII-LKFASIELDSDQQQKPFIKCVVEPILPDDETEEYKKYVGEV 68
           VT+KPK K   + +  II LKFAS+ELDS++Q K FIKC  + +   DE  EYKKY+GE+
Sbjct: 9   VTMKPK-KDEQFRWVEIIYLKFASVELDSNEQPKEFIKCEAQ-LQEVDELVEYKKYIGEI 68

Query: 69  EVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSKSVLDQRRV 128
            V EG+GEIGAVIVEL+      E+FIDTIS+  ++   S TFSCKSWVQ K ++D RR+
Sbjct: 69  VVAEGFGEIGAVIVELQEGVN--ERFIDTISVEAEEPPISITFSCKSWVQPKGLIDHRRI 128

Query: 129 FFST-KSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYDFYNDLGDC 188
           FFS+ KSYL  KTP GL+KLR EDL+NLRG K D +VD NERKAFERIYDYD YNDLGD 
Sbjct: 129 FFSSNKSYLLGKTPRGLVKLREEDLANLRGEKEDGSVDRNERKAFERIYDYDLYNDLGDP 188

Query: 189 DGPAEWKRPVLGGSD-HPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEFSEVKQHYF 248
           D   + KRPVLGGS+ +PYPRRCRTGR   + DP SEKR +E FY+PRDEEFSE+KQ   
Sbjct: 189 DLSMDLKRPVLGGSEEYPYPRRCRTGRPPTQNDPASEKRVEEWFYIPRDEEFSEIKQS-- 248

Query: 249 PSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKL-LTFNLSTIVTPDKPDLHSNS 308
            S++P NK LL K   SDLP I      GIK P A  K+ L  N+S+IV   +P   + +
Sbjct: 249 -SNQPRNKKLLKKAPSSDLPEI------GIKMPSAASKINLQCNVSSIVCSHQPPAAAIN 308

Query: 309 NSSVPSS-LLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLPLMSELDPDT 368
           +S VPSS L+  PPPESY+RD+Y+WLSD EFAR TLAGLNPYSIQLV  LP MS+LD   
Sbjct: 309 SSDVPSSTLVELPPPESYKRDQYNWLSDIEFARLTLAGLNPYSIQLVKSLPFMSKLDEGD 368

Query: 369 YGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKINGTTLYGSR 428
           YGP+ES F   +VQEL+GC I V +A+  KRLFV+DYHDTLMPYV +VRKI GTTLYGSR
Sbjct: 369 YGPRESKFTPERVQELLGCCITVGKALADKRLFVVDYHDTLMPYVRRVRKIKGTTLYGSR 428

Query: 429 TLFFLKSDDTLIPLGIELTRPPVDGY-PQWKQIFTPGIEATDLWLWRIAKAHVLAHDSCI 488
           TLFFL SD TL+PLGIELTRPP+D +  QWK++FTPG  +TDLWLWR AKAHVL+HDSCI
Sbjct: 429 TLFFLNSDGTLMPLGIELTRPPMDEHLEQWKEVFTPGTNSTDLWLWRFAKAHVLSHDSCI 488

Query: 489 HQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARESLINAGGII 548
           HQLVIHWLRAHCCMEPYAIATNRQLST+HPIYRLLHPHFRYNMRINANAR++LINAGGII
Sbjct: 489 HQLVIHWLRAHCCMEPYAIATNRQLSTMHPIYRLLHPHFRYNMRINANARKNLINAGGII 548

Query: 549 ESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAERKKGDYGRDVLELAIKDYPF 608
           E+ FS ASYSVELSSS Y++ WRFD QALPEDLI RGMAERK+   GRDVLEL IKDYPF
Sbjct: 549 ENAFSAASYSVELSSSAYKE-WRFDMQALPEDLIHRGMAERKRDSEGRDVLELTIKDYPF 608

Query: 609 ANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDKKEGWPTLKT 668
           ANDGLLLWNALLEWVTEYVNHYYGD+ENAVIND ELQAWW EIQEKGHPDK+EGW  L+T
Sbjct: 609 ANDGLLLWNALLEWVTEYVNHYYGDEENAVINDTELQAWWGEIQEKGHPDKREGWFALRT 668

Query: 669 RNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDY-HQLPEEFIDL 728
           R+DLIKIASTIAWVGSGHHASVNF+QYAY GY PNRP+IARTN+LTE++  +  ++FI+ 
Sbjct: 669 RDDLIKIASTIAWVGSGHHASVNFLQYAYGGYMPNRPTIARTNMLTENHSDKFLKDFINQ 728

Query: 729 PENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSISKAFKRFQASL 788
           PE +L ++FPS  QA++V  TM LLS HSPDEEYIGD IEPAWAL+ SISKAF++F+A+L
Sbjct: 729 PEKKLNELFPSDAQAAIVKQTMFLLSIHSPDEEYIGDAIEPAWALDQSISKAFEKFKANL 788

Query: 789 KDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVS 830
            DLE++ID+ N+N  LKNR+GA ++PY+ +KP S  GITG GVPYSVS
Sbjct: 789 TDLEKKIDELNQNKDLKNRYGAAIIPYEAMKPRSKPGITGSGVPYSVS 822

BLAST of Cucsa.153660 vs. NCBI nr
Match: gi|778693083|ref|XP_011653576.1| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 551/839 (65.67%), Postives = 657/839 (78.31%), Query Frame = 1

Query: 1   MGVEVIASVTVKPKTKGPTYPYAN-IILKFASIELDSDQQQKPFIKCVVEPILPDDETEE 60
           M   +IA V+V PK+ G    Y + ++L FAS +LD D QQKP I+ V E ++ ++ T+ 
Sbjct: 1   MASNIIAIVSVIPKSNGSIIHYNDSLLLFFASSDLDRDGQQKPLIRGVAE-LIKNESTKA 60

Query: 61  YKKYVGEVEVPEGYGEIGAVIVELEGDTTKYEKFIDTISITDKKSRNSTTFSCKSWVQSK 120
             KYV +V+VP+GYGEIGAV+VELE  T   EKFIDT+ + +  S+N+ TFSC SWVQ K
Sbjct: 61  KHKYVAKVQVPKGYGEIGAVVVELE--THSSEKFIDTVIVANSTSQNTITFSCTSWVQPK 120

Query: 121 SVL-DQRRVFFSTKSYLPEKTPGGLLKLRAEDLSNLRGIKADETVDMNERKAFERIYDYD 180
           S++ DQRR+FFSTKSYLPE TP GLLKLRAEDL NLRG K D TVD NER  FERIYDYD
Sbjct: 121 SLIPDQRRIFFSTKSYLPEDTPAGLLKLRAEDLENLRGQKQDGTVDTNERTKFERIYDYD 180

Query: 181 FYNDLGDCDGPAEWKRPVLGGS-DHPYPRRCRTGRLHCKTDPVSEKRSKERFYVPRDEEF 240
            YNDLGD D   +WKRPVLGGS  +PYPRRCRTGR H  +DP SE+RS + FYVPRDE F
Sbjct: 181 LYNDLGDPDVDTKWKRPVLGGSVQYPYPRRCRTGRPHTVSDPKSEQRSAKSFYVPRDEAF 240

Query: 241 SEVKQHYFPSSEPDNKDLLGKDSFSDLPHIESMFREGIKAPHAPHKLLTFN-LSTIVTPD 300
           SEVKQ  FPS+   NKD LG   F+D   I+ MFR+GI  P  PH++L FN LST+   +
Sbjct: 241 SEVKQTMFPSNS-GNKDKLGTKPFTDFTQIDLMFRDGIALPSNPHEILQFNILSTLNASN 300

Query: 301 KPDLHSNSNSSVPSS--LLHYPPPESYRRDRYSWLSDTEFARQTLAGLNPYSIQLVTRLP 360
           +P         V  +  ++ +PPPE+ +RD+++WLSD+EFARQT+AGLNPY IQLV   P
Sbjct: 301 QPAPPPVQPIPVQPNTPVIKFPPPEALKRDKFNWLSDSEFARQTIAGLNPYCIQLVKSWP 360

Query: 361 LMSELDPDTYGPQESAFNHTKVQELIGCSIEVNEAIELKRLFVLDYHDTLMPYVNKVRKI 420
           L SELDP  YGPQESAF    VQ+LIG SI V EAI  K+LFVLDYHDTLM YV KVR I
Sbjct: 361 LKSELDPVEYGPQESAFTTELVQKLIGSSITVEEAIAQKKLFVLDYHDTLMQYVEKVRSI 420

Query: 421 NGTTLYGSRTLFFLKSDDTLIPLGIELTRPPVDGYPQWKQIFTPGIEATDLWLWRIAKAH 480
             TTLYGSRTLFFL+ DDTL P+GIELTRPP+DG PQWKQ+F+P  + T+ WLWR+AKAH
Sbjct: 421 KRTTLYGSRTLFFLEYDDTLTPMGIELTRPPMDGKPQWKQVFSPSTQGTNAWLWRLAKAH 480

Query: 481 VLAHDSCIHQLVIHWLRAHCCMEPYAIATNRQLSTVHPIYRLLHPHFRYNMRINANARES 540
           VLAHDSC+HQLVIHWLR HCCMEPYAIA NRQLST+HPIYRLLHPHFR+NMRINANARES
Sbjct: 481 VLAHDSCVHQLVIHWLRTHCCMEPYAIALNRQLSTMHPIYRLLHPHFRFNMRINANARES 540

Query: 541 LINAGGIIESTFSTASYSVELSSSVYRDQWRFDEQALPEDLIRRGMAER-KKGDYGRDVL 600
           L NAGGIIESTFS  +YS+E SS VY+ +W+FD QALPEDLIRRGMAER     +G   L
Sbjct: 541 LNNAGGIIESTFSVGAYSMEFSSLVYKKEWQFDTQALPEDLIRRGMAERDASAPHG---L 600

Query: 601 ELAIKDYPFANDGLLLWNALLEWVTEYVNHYYGDDENAVINDKELQAWWNEIQEKGHPDK 660
           +LAIKDYPFANDGL+LW  L+EW++EYVNHYY  DE A+ NDKEL+AWW EI EKGHPDK
Sbjct: 601 KLAIKDYPFANDGLILWEVLVEWISEYVNHYY-PDEKAIKNDKELKAWWKEIMEKGHPDK 660

Query: 661 KE--GWPTLKTRNDLIKIASTIAWVGSGHHASVNFIQYAYAGYTPNRPSIARTNILTEDY 720
           K+  GWP+LKT  DLI+I STIAWVG GHH++VNFIQYA+AGY PNRPSI RTN+ TEDY
Sbjct: 661 KKEAGWPSLKTPKDLIQIVSTIAWVGCGHHSAVNFIQYAHAGYFPNRPSITRTNMPTEDY 720

Query: 721 HQLPEEFIDLPENELLQVFPSVDQASVVTTTMILLSAHSPDEEYIGDEIEPAWALEPSIS 780
            Q+PE FI+ PE+ +L+ FPS+ QAS V  TM++LSAHSPDEEYI  +IEPAWA +P I+
Sbjct: 721 DQIPEAFINNPESVILEAFPSIAQASAVAQTMLILSAHSPDEEYIAKKIEPAWAEDPMIA 780

Query: 781 KAFKRFQASLKDLEQQIDKNNKNNKLKNRHGAGVVPYDVLKPTSTYGITGRGVPYSVST 831
           KAF +F+  L +LE+ ID+ N+NN+LKNR GAG+VPY+VLKPTS +G+TG+GVPYSVST
Sbjct: 781 KAFDKFKMKLDELEKTIDQRNENNELKNRRGAGLVPYEVLKPTSGFGVTGKGVPYSVST 831