Cucsa.152690.2 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.152690.2
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Mediator of RNA polymerase II transcription subunit 21
Location	scaffold01124 : 81612 .. 84096 (+)
Sequence length	886

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACGTTCTAAAACTAGAAAATTAGGAAGGTATAAGGAAAAGAAGGAACCCTAATTCATCTGCTTTTTCTCCGACTCACGTTCAACCGCCCAGCCCCTTCCTTGTCTCCGGTAGCATCTCGATGACAGTCCTTTGAGATAAACGGCGGATCGTACTCTCACTCACTCAGTTTCTGGAACTGATTTGCTGATTAAAAGGAGCGATATGTGTATTATTTGCGGAATCATCTTCTGAACAATTCATCCTAGGGATAAGGTTGAAGATGGATATAATTTCCCAATTGCAGGAACAAGTTAACACAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTGCAAAGAGATGCTCCTCCAGTTCGTTTATCCCCTAATTATCCCGAACCACCGTCTCAGGAGCCTACAGAGGATAATGCAACCATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAGCAGGTGCGTATCATTTATTCTGCACTTTAATACTTCGGGGTGGAAAATATGAATGAGTTAGAGTACCTCTTGTTAATAAACTTTCCACGCATTGAAACATCAAGAAAAATATTTGCATTTTCCATTTGAGGAAATTCAGTGTCCTAGATTAACTTTACATGCATTCTGTCTAGTGTCTACTGGGTAAGTAGCCGAGAATGCAGTATCTACTTGAAGCACCCTTGTGTTGAGGTTGGAAGAGAAAGAAAAATCAAGGCAGCAGGTTTCATGCTACTTGTTTGAGATGAGTACATATTCTAATGCTATGATATATGATACAATCCACAAATTTGGTATTGAAAACTTCAGTCTATTTTTCCCATCTAACATCAAATACTACTGAGGTAGGTATGGCTATTCGATCGGCAATAAAaCAAaGTACTATGGTTCTTTTTTTtAGTTATCCTTTtACTTTTtAACTGCTTGTTATGTATATTTAGGCCCACTTTAAGGTGTTCTTTAAGTGTTGATATTTTGGAATCTGATACTCAAACCCTGTATTTGAGCAATTTTGCTGAGAGAGTTTTGATTTTCACAAGTTTCCATCGTCGAACTCAGCAATGCATTTAGGTTTGTTCTTCCGAATAATCTTTTAAGGATTATATAGAATTATAAAATAGACTGATTCTGTCCACGTTAAGTTTGAAAATCTCAGTGTGATAGTATTAAGTAATAGAAACGTACAAAGTTTTCCAACTCTGCTGGACTTTTAATGACTTCTAGATGTGATTCTACAACGAGTTAGATTTGTTCTATATATTCTCTCATTTTCTGTATTTTTTACGTTTACCTCTTGATTGGTATGTTATTTTATTAGTTTTTTGAGGAGTTGCAAGGAGCCAACTGAGGAATTTCTCCTCCATCCACTTTTCTCTCTCGGAGAAATGGCTGTTTTCATAGTAGGTTGGAGTATATGCTAATTTGTGGGGTTTGGGGTGCGAGAGAAATGATAGAATCTTCAAGGGAGAAATAACCGAATCTTGGATCTTGAAGATTCGTTTTTAATAATTAATCGCTTAGTCACATTTCACTTGATTGGAGCCCTTCCCCTTCTTATAGTTAGCTACACTTTCTGTGAACACCATTCTGTATGGTTATGTATTCTTTCACCTTGTTTTTCAACAAAAGTCTAGTTGTACATCAAAAACAAAATTTATCAGCATTTTGAACAAAAGAAAGGGATGGGATCTTGCTTCATTTATTTGCAATTATCCAATTGAACATGTGTACCTTTTCTTAAAAAAaTTATTGTCAATATTTTCTAGCACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGTATGAATTCTCATATTCTGATGTTGATATAAGATCAAGAGTTGTTGTGTTGTTTACTTCTCTCAGTATGTATTTCAGGCTGAGAACGATGTTGTAGGTCAAGAACTTCAAAAACAGCTACAAGCAGCAGGTGTTATCTCTGTCTTCTTGTTAATCTCTACATTTTCAAACCGTGCCCCGGTGTGGTTACATAAGACTGATGTTGTTTGGCTGGCGACTTACTTTAGTTGAAATAATTCTATTTAATAATTTTAGCCCTAAATGTAAATTTCTCATGTTCACTTCATTCTATTGAACATAAGGATATTTTGCGCAACTAACAATCTTACCCAAACTGTGTTTCAGAGAAAGAATTAGTACAAGTTCAGGAGCTGTTCAGCCAAGTGGCAGATAATTGCTTGAACTTAATGAAAGCAGATTGAAAGAGTGGGGGAATTTTCCTATGCTACCAATGTATGGAAATTTTCCGTATCTCCCCGTGCATTTCTGCCGCATGACTTTTTATTTTTATATTACATATATGTAGAAATATACGTGAATCTACAGTTTATGGAATAAAAATACTTTTATCCATTGTATTAATTTTTTTTtATGACGGATTGATATTTTCATCAATATTTCTACGTTTTC

mRNA sequence

GACGTTCTAAAACTAGAAAATTAGGAAGGTATAAGGAAAAGAAGGAACCCTAATTCATCTGCTTTTTCTCCGACTCACGTTCAACCGCCCAGCCCCTTCCTTGTCTCCGGTAGCATCTCGATGACAGTCCTTTGAGATAAACGGCGGATCGTACTCTCACTCACTCAGTTTCTGGAACTGATTTGCTGATTAAAAGGAGCGATATGTGTATTATTTGCGGAATCATCTTCTGAACAATTCATCCTAGGGATAAGGTTGAAGATGGATATAATTTCCCAATTGCAGGAACAAGTTAACACAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTGCAAAGAGATGCTCCTCCAGTTCGTTTATCCCCTAATTATCCCGAACCACCGTCTCAGGAGCCTACAGAGGATAATGCAACCATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAGCAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGTTGTAGGTCAAGAACTTCAAAAACAGCTACAAGCAGCAGAGAAAGAATTAGTACAAGTTCAGGAGCTGTTCAGCCAAGTGGCAGATAATTGCTTGAACTTAATGAAAGCAGATTGAAAGAGTGGGGGAATTTTCCTATGCTACCAATGTATGGAAATTTTCCGTATCTCCCCGTGCATTTCTGCCGCATGACTTTTTATTTTTATATTACATATATGTAGAAATATACGTGAATCTACAGTTTATGGAATAAAAATACTTTTATCCATTGTATTAATTTTTTTTTATGACGGATTGATATTTTCATCAATATTTCTACGTTTTC

Coding sequence (CDS)

ATGGATATAATTTCCCAATTGCAGGAACAAGTTAACACAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTGCAAAGAGATGCTCCTCCAGTTCGTTTATCCCCTAATTATCCCGAACCACCGTCTCAGGAGCCTACAGAGGATAATGCAACCATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAGCAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGTTGTAGGTCAAGAACTTCAAAAACAGCTACAAGCAGCAGAGAAAGAATTAGTACAAGTTCAGGAGCTGTTCAGCCAAGTGGCAGATAATTGCTTGAACTTAATGAAAGCAGATTGA

Protein sequence

MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQVQELFSQVADNCLNLMKAD*

Homology

[Close]

BLAST of Cucsa.152690.2 vs. Swiss-Prot
Match: MED21_ARATH (Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana GN=MED21 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.0e-53
Identity = 109/139 (78.42%), Postives = 119/139 (85.61%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNAT-IAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP+     D+AT   EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQ 120
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END+VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLMKAD 139
           VQELF Q ADNCLN+ K +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

[Close]

BLAST of Cucsa.152690.2 vs. Swiss-Prot
Match: MED21_DANRE (Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio GN=med21 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 7.2e-07
Identity = 38/136 (27.94%), Postives = 66/136 (48.53%), Query Frame = 1


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSP-----NYPEPPSQEPTEDNATIAEQ 61
           D ++QLQ+ VN++A    N  G LQ+ APP   S      N  +P +  PTE+ A     
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSN--PTEEYA----- 62

Query: 62  PKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKE 121
            +L +A + + AK  D L+ +LP  +     Q   + +L+ EN      L++ +   +  
Sbjct: 63  -QLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDAL 122

Query: 122 LVQVQELFSQVADNCL 133
           L ++Q   + +A + L
Sbjct: 123 LEKIQTALADIAQSQL 130

[Close]

BLAST of Cucsa.152690.2 vs. Swiss-Prot
Match: MED21_ANOGA (Mediator of RNA polymerase II transcription subunit 21 OS=Anopheles gambiae GN=MED21 PE=3 SV=5)

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-06
Identity = 38/148 (25.68%), Postives = 70/148 (47.30%), Query Frame = 1


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPV------RLSPNYPE---------PPSQEP 61
           D ++QLQ+ VN  A    N+ G LQ+ + P       R     P+         P  Q+ 
Sbjct: 3   DRLTQLQDTVNQQAEHFCNSIGILQQCSVPSKFAGFERTGSQTPQQQVHQQQQLPQQQQQ 62

Query: 62  TEDNATIAEQPKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQEL 121
            +      + P+L S  + + AK  D L+ +LP  +   E Q++ +  L+AEN    ++L
Sbjct: 63  QQQPQQQEDFPQLFSTLISRCAKDIDTLIESLPSEESSIELQVQSLQRLEAENKESAEKL 122

Query: 122 QKQLQAAEKELVQVQELFSQVADNCLNL 135
           ++ ++  E  L ++Q   S +A + L++
Sbjct: 123 EEIVRKGELLLEKIQAALSDIAQSQLDM 150

[Close]

BLAST of Cucsa.152690.2 vs. Swiss-Prot
Match: MED21_BOVIN (Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus GN=MED21 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 4.7e-06
Identity = 36/136 (26.47%), Postives = 66/136 (48.53%), Query Frame = 1


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPP-----VRLSPNYPEPPSQEPTEDNATIAEQ 61
           D ++QLQ+ VN++A    N  G LQ+  PP     ++ + N  +P +  PTE+ A     
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPAN--PTEEYA----- 62

Query: 62  PKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKE 121
            +L +A + + AK  D L+ +LP  +     Q   + +L+ EN      L+  +   +  
Sbjct: 63  -QLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATSLEDVVYRGDML 122

Query: 122 LVQVQELFSQVADNCL 133
           L ++Q   + +A + L
Sbjct: 123 LEKIQSALADIAQSQL 130

[Close]

BLAST of Cucsa.152690.2 vs. TrEMBL
Match: A0A0A0KZ00_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268030 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 6.7e-68
Identity = 137/138 (99.28%), Postives = 137/138 (99.28%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELFSQVADNCLNL KAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Cucsa.152690.2 vs. TrEMBL
Match: M1BD21_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400016459 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.3e-58
Identity = 119/138 (86.23%), Postives = 124/138 (89.86%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED+  +AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELF+Q  DNCLNL K +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Cucsa.152690.2 vs. TrEMBL
Match: A0A0V0HDL0_SOLCH (Putative mediator of RNA polymerase II transcription subunit 21-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.3e-58
Identity = 119/138 (86.23%), Postives = 124/138 (89.86%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED+  +AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELF+Q  DNCLNL K +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Cucsa.152690.2 vs. TrEMBL
Match: K4B9R4_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 6.3e-58
Identity = 118/138 (85.51%), Postives = 123/138 (89.13%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED+A +AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSANVAEQPKQM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAA VKAAKQFD LVAALPLSDG EE QLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDVLVAALPLSDGSEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELF+Q  DNCLNL K +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Cucsa.152690.2 vs. TrEMBL
Match: A0A0S2LKQ9_REHGL (Mediator of RNA polymerase II transcription subunit 21 OS=Rehmannia glutinosa GN=Med21 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 9.1e-57
Identity = 118/139 (84.89%), Postives = 126/139 (90.65%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQ-EPTEDNATIAEQPKL 60
           MDIISQLQEQVNTIAA+AFNTFG+LQRDAPPV+LSPNYPEPP+   P ED A +AEQPKL
Sbjct: 1   MDIISQLQEQVNTIAAIAFNTFGSLQRDAPPVQLSPNYPEPPANGNPVEDAANLAEQPKL 60

Query: 61  MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQ 120
           +SA LVKAAKQFDALVAALPLS+GGEE QLKRIAELQAEND VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAELVKAAKQFDALVAALPLSEGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELRQ 120

Query: 121 VQELFSQVADNCLNLMKAD 139
           VQELFSQ ADNCLNL K D
Sbjct: 121 VQELFSQAADNCLNLKKPD 139

[Close]

BLAST of Cucsa.152690.2 vs. TAIR10
Match: AT4G04780.1 (AT4G04780.1 mediator 21)

HSP 1 Score: 210.7 bits (535), Expect = 5.8e-55
Identity = 109/139 (78.42%), Postives = 119/139 (85.61%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNAT-IAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP+     D+AT   EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQ 120
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END+VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLMKAD 139
           VQELF Q ADNCLN+ K +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

[Close]

BLAST of Cucsa.152690.2 vs. NCBI nr
Match: gi|449463842|ref|XP_004149640.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus])

HSP 1 Score: 264.6 bits (675), Expect = 9.6e-68
Identity = 137/138 (99.28%), Postives = 137/138 (99.28%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELFSQVADNCLNL KAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Cucsa.152690.2 vs. NCBI nr
Match: gi|659097951|ref|XP_008449895.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo])

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-66
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTED+ATIAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAE+ELVQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELFSQVADNCLNL KAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Cucsa.152690.2 vs. NCBI nr
Match: gi|698553696|ref|XP_009770008.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21-like isoform X1 [Nicotiana sylvestris])

HSP 1 Score: 234.2 bits (596), Expect = 1.4e-58
Identity = 120/138 (86.96%), Postives = 124/138 (89.86%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED A +AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDAANVAEQPKQM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELF+Q  DNCLNL K +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Cucsa.152690.2 vs. NCBI nr
Match: gi|565362742|ref|XP_006348104.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum tuberosum])

HSP 1 Score: 233.8 bits (595), Expect = 1.8e-58
Identity = 119/138 (86.23%), Postives = 124/138 (89.86%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED+  +AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAA VKAAKQFDALVAALPLSDGGEE QLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDALVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELF+Q  DNCLNL K +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

[Close]

BLAST of Cucsa.152690.2 vs. NCBI nr
Match: gi|970005966|ref|XP_015063828.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum pennellii])

HSP 1 Score: 232.3 bits (591), Expect = 5.3e-58
Identity = 118/138 (85.51%), Postives = 123/138 (89.13%), Query Frame = 1


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP   PTED+  +AEQPK M
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPANPTEDSTNVAEQPKQM 60

Query: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120
           SAA VKAAKQFD LVAALPLSDGGEE QLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAAFVKAAKQFDVLVAALPLSDGGEEAQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLMKAD 139
           QELF+Q  DNCLNL K +
Sbjct: 121 QELFNQATDNCLNLKKPE 138

The following BLAST results are available for this feature:

BLAST of Cucsa.152690.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MED21_ARATH	1.0e-53	78.42	Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana G...	[more]
MED21_DANRE	7.2e-07	27.94	Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio GN=med21 P...	[more]
MED21_ANOGA	1.6e-06	25.68	Mediator of RNA polymerase II transcription subunit 21 OS=Anopheles gambiae GN=M...	[more]
MED21_BOVIN	4.7e-06	26.47	Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus GN=MED21 PE...	[more]

BLAST of Cucsa.152690.2 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ00_CUCSA	6.7e-68	99.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G268030 PE=4 SV=1	[more]
M1BD21_SOLTU	1.3e-58	86.23	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400016459 PE=4 SV=1	[more]
A0A0V0HDL0_SOLCH	1.3e-58	86.23	Putative mediator of RNA polymerase II transcription subunit 21-like OS=Solanum ...	[more]
K4B9R4_SOLLC	6.3e-58	85.51	Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1	[more]
A0A0S2LKQ9_REHGL	9.1e-57	84.89	Mediator of RNA polymerase II transcription subunit 21 OS=Rehmannia glutinosa GN...	[more]

BLAST of Cucsa.152690.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G04780.1	5.8e-55	78.42	mediator 21	[more]

BLAST of Cucsa.152690.2 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449463842\|ref\|XP_004149640.1\|	9.6e-68	99.28	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis sativ...	[more]
gi\|659097951\|ref\|XP_008449895.1\|	1.1e-66	97.83	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo]	[more]
gi\|698553696\|ref\|XP_009770008.1\|	1.4e-58	86.96	PREDICTED: mediator of RNA polymerase II transcription subunit 21-like isoform X...	[more]
gi\|565362742\|ref\|XP_006348104.1\|	1.8e-58	86.23	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum tuber...	[more]
gi\|970005966\|ref\|XP_015063828.1\|	5.3e-58	85.51	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Solanum penne...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR021384	Mediator_Med21

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0043078	polar nucleus
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.152690	Cucsa.152690	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.152690.2	Cucsa.152690.2-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.152690.2.five_prime_UTR.1	Cucsa.152690.2.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.152690.2.CDS.1	Cucsa.152690.2.CDS.1	CDS
Cucsa.152690.2.CDS.2	Cucsa.152690.2.CDS.2	CDS
Cucsa.152690.2.CDS.3	Cucsa.152690.2.CDS.3	CDS
Cucsa.152690.2.CDS.4	Cucsa.152690.2.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.152690.2.three_prime_UTR.1	Cucsa.152690.2.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021384	Mediator complex, subunit Med21	PFAM	PF11221	Med21	coord: 1..128 score: 1.4
None	No IPR available	unknown	Coil	Coil	coord: 92..130 scor
None	No IPR available	PANTHER	PTHR13381	RNA POLYMERASE II HOLOENZYME COMPONENT SRB7	coord: 1..134 score: 9.4
None	No IPR available	unknown	SSF140718	Mediator hinge subcomplex-like	coord: 1..126 score: 6.8