Cucsa.151990.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.151990.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPseudouridine synthase family protein
Locationscaffold01119 : 295101 .. 295487 (+)
Sequence length322
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTCTCATCTTCTACTGCCTCCACTGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACGTTACTCTCTGTTTCTCTTGACCATCCTCTTGTGAATCCTTCACCATTTCTCATTAACTCACTAGTTTTGACAATTCAGGACTACAAATGCAACGCAACCAACTGTCACATCCAA

mRNA sequence

ATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTCTCATCTTCTACTGCCTCCACTGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACTTTTGACAATTCAGGACTACAAATGCAACGCAACCAACTGTCACATCCAA

Coding sequence (CDS)

ATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTCTCATCTTCTACTGCCTCCACTGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACTTTTGACAATTCAGGACTACAAATGCAACGCAACCAACTGTCACATCCAA

Protein sequence

MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLSHPX
BLAST of Cucsa.151990.1 vs. Swiss-Prot
Match: PUSH_ARATH (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.3e-08
Identity = 42/109 (38.53%), Postives = 59/109 (54.13%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           M+VS LR+P+        S  F +P     +R   + PALL F  SS+A+    ST  +P
Sbjct: 1   MAVSFLRIPIP-------SRVFWYP-----TRLIRVTPALLRF--SSSAAFQPSSTSLSP 60

Query: 61  DQ-----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQL 105
                    KW+++RKKKVV+RIGYVGTDYRG     D+  ++    +L
Sbjct: 61  PSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGEL 95

BLAST of Cucsa.151990.1 vs. TrEMBL
Match: A0A0A0LVB6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G533670 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 9.6e-46
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
          MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP
Sbjct: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFT 92
          DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFT
Sbjct: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFT 91

BLAST of Cucsa.151990.1 vs. TrEMBL
Match: M5WP30_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004352mg PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 6.9e-12
Identity = 47/105 (44.76%), Postives = 62/105 (59.05%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           M++SSLRLP S W+ +     FRF  P+      PLR  L++  SS +++    ST    
Sbjct: 1   MAISSLRLPFSLWLPRLPIQSFRFSKPIL-----PLRLNLIYCSSSISSTAQQTSTH--- 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLS 106
             L KWQ+ RKKKVVLR+GYVG+DYR         GLQMQR++ S
Sbjct: 61  --LEKWQSVRKKKVVLRVGYVGSDYR---------GLQMQRDEHS 86

BLAST of Cucsa.151990.1 vs. TrEMBL
Match: A0A068U688_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00016610001 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 9.0e-12
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWIT-KASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAF----- 60
           M+V  LRLPLSPW+  +A+ +  +F +P       PL+P LL F +S+ AS  A      
Sbjct: 1   MAVIPLRLPLSPWLAARAAPTTLQFFNP-----NKPLKPFLLRFSTSAAASDAAAQDSVP 60

Query: 61  ---STRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLSHP 108
              S+  +P    KW+ FRKKKVV+R+GYVG+DYR         GLQMQR++ + P
Sbjct: 61  LPPSSSPSPPPQNKWEPFRKKKVVMRVGYVGSDYR---------GLQMQRDEHALP 102

BLAST of Cucsa.151990.1 vs. TrEMBL
Match: F6GUG3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g07710 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 2.6e-11
Identity = 48/106 (45.28%), Postives = 64/106 (60.38%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           M VSSLR PL PWI    +S  RF +P  + +        LH  SSS+++     T   P
Sbjct: 1   MVVSSLRFPLFPWIISNHTSNLRFSNPRILPQWS------LHCSSSSSSTV----TESNP 60

Query: 61  DQ-LRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLS 106
           DQ ++KW++FRKKKVV+R+GYVGT+YR         GLQMQR++ S
Sbjct: 61  DQSVQKWESFRKKKVVMRVGYVGTNYR---------GLQMQRDESS 87

BLAST of Cucsa.151990.1 vs. TrEMBL
Match: A5C2V2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034891 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 2.6e-11
Identity = 48/106 (45.28%), Postives = 64/106 (60.38%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           M VSSLR PL PWI    +S  RF +P  + +        LH  SSS+++     T   P
Sbjct: 1   MVVSSLRFPLFPWIISNHTSNLRFSNPRILPQWS------LHCSSSSSSTV----TESNP 60

Query: 61  DQ-LRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLS 106
           DQ ++KW++FRKKKVV+R+GYVGT+YR         GLQMQR++ S
Sbjct: 61  DQSVQKWESFRKKKVVMRVGYVGTNYR---------GLQMQRDESS 87

BLAST of Cucsa.151990.1 vs. TAIR10
Match: AT2G30320.1 (AT2G30320.1 Pseudouridine synthase family protein)

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-10
Identity = 42/109 (38.53%), Postives = 59/109 (54.13%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           M+VS LR+P+        S  F +P     +R   + PALL F  SS+A+    ST  +P
Sbjct: 1   MAVSFLRIPIP-------SRVFWYP-----TRLIRVTPALLRF--SSSAAFQPSSTSLSP 60

Query: 61  DQ-----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQL 105
                    KW+++RKKKVV+RIGYVGTDYRG     D+  ++    +L
Sbjct: 61  PSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGEL 95

BLAST of Cucsa.151990.1 vs. NCBI nr
Match: gi|449454977|ref|XP_004145230.1| (PREDICTED: putative tRNA pseudouridine synthase [Cucumis sativus])

HSP 1 Score: 195.3 bits (495), Expect = 5.6e-47
Identity = 98/107 (91.59%), Postives = 98/107 (91.59%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP
Sbjct: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLSHP 108
           DQLRKWQAFRKKKVVLRIGYVGTDYR         GLQMQRNQLSHP
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYR---------GLQMQRNQLSHP 98

BLAST of Cucsa.151990.1 vs. NCBI nr
Match: gi|700210764|gb|KGN65860.1| (hypothetical protein Csa_1G533670 [Cucumis sativus])

HSP 1 Score: 190.7 bits (483), Expect = 1.4e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1

Query: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
          MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP
Sbjct: 1  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60

Query: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFT 92
          DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFT
Sbjct: 61 DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFT 91

BLAST of Cucsa.151990.1 vs. NCBI nr
Match: gi|659114949|ref|XP_008457308.1| (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 173.7 bits (439), Expect = 1.7e-40
Identity = 88/107 (82.24%), Postives = 92/107 (85.98%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           MSVSSLRLPLSPWITK+SSSCFRFP PVFV RTP LRP LL+ FSS+ ASTDAFST+CAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLSHP 108
           DQLRKWQAFRKKKVVLRIGYVGTDYR         GLQMQRNQLSHP
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYR---------GLQMQRNQLSHP 98

BLAST of Cucsa.151990.1 vs. NCBI nr
Match: gi|595837032|ref|XP_007207376.1| (hypothetical protein PRUPE_ppa004352mg [Prunus persica])

HSP 1 Score: 78.2 bits (191), Expect = 9.9e-12
Identity = 47/105 (44.76%), Postives = 62/105 (59.05%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAFSTRCAP 60
           M++SSLRLP S W+ +     FRF  P+      PLR  L++  SS +++    ST    
Sbjct: 1   MAISSLRLPFSLWLPRLPIQSFRFSKPIL-----PLRLNLIYCSSSISSTAQQTSTH--- 60

Query: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLS 106
             L KWQ+ RKKKVVLR+GYVG+DYR         GLQMQR++ S
Sbjct: 61  --LEKWQSVRKKKVVLRVGYVGSDYR---------GLQMQRDEHS 86

BLAST of Cucsa.151990.1 vs. NCBI nr
Match: gi|661892912|emb|CDP04070.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-11
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 1

Query: 1   MSVSSLRLPLSPWIT-KASSSCFRFPDPVFVSRTPPLRPALLHFFSSSTASTDAF----- 60
           M+V  LRLPLSPW+  +A+ +  +F +P       PL+P LL F +S+ AS  A      
Sbjct: 1   MAVIPLRLPLSPWLAARAAPTTLQFFNP-----NKPLKPFLLRFSTSAAASDAAAQDSVP 60

Query: 61  ---STRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTFDNSGLQMQRNQLSHP 108
              S+  +P    KW+ FRKKKVV+R+GYVG+DYR         GLQMQR++ + P
Sbjct: 61  LPPSSSPSPPPQNKWEPFRKKKVVMRVGYVGSDYR---------GLQMQRDEHALP 102

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PUSH_ARATH1.3e-0838.53Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana GN=At2g30320 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A0A0LVB6_CUCSA9.6e-46100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G533670 PE=4 SV=1[more]
M5WP30_PRUPE6.9e-1244.76Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004352mg PE=4 SV=1[more]
A0A068U688_COFCA9.0e-1242.24Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00016610001 PE=4 SV=1[more]
F6GUG3_VITVI2.6e-1145.28Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g07710 PE=4 SV=... [more]
A5C2V2_VITVI2.6e-1145.28Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034891 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G30320.17.6e-1038.53 Pseudouridine synthase family protein[more]
Match NameE-valueIdentityDescription
gi|449454977|ref|XP_004145230.1|5.6e-4791.59PREDICTED: putative tRNA pseudouridine synthase [Cucumis sativus][more]
gi|700210764|gb|KGN65860.1|1.4e-45100.00hypothetical protein Csa_1G533670 [Cucumis sativus][more]
gi|659114949|ref|XP_008457308.1|1.7e-4082.24PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo][more]
gi|595837032|ref|XP_007207376.1|9.9e-1244.76hypothetical protein PRUPE_ppa004352mg [Prunus persica][more]
gi|661892912|emb|CDP04070.1|1.3e-1142.24unnamed protein product [Coffea canephora][more]
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.151990Cucsa.151990gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.151990.1Cucsa.151990.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.151990.1.CDS.1Cucsa.151990.1.CDS.1CDS
Cucsa.151990.1.CDS.2Cucsa.151990.1.CDS.2CDS