Cucsa.108250.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.108250.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	NADH dehydrogenase 1 alpha subcomplex subunit 5
Location	scaffold00931 : 431566 .. 433342 (+)
Sequence length	870

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGCCCCTCCATCCTCCTGAAGCGACTGGCTCGTTACCACTGAGAAGCGTGATCAAGAGGAAGAGACAAGAGTTTTCCGACGATGTTCCTGCGAGCGATCGGACGGCCATTATTGGCCAAAGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTTGTTCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAGTAAAACCCTAAAGGAGATCCAGGCCGTCCCCGAGGACGAAGGATACCGTAAGGCGGTTGAGAGCTTTACGCGGCACCGCTTGAAGGTCTGCCAGGAAGAAGAGGATTGGGAGAATATCGAGAAGCGGCTTGGCTGTGGTCAGGTCGAGGAGCTAATCGAGGAGGCCCAGGACGAGCTCAAACTCATTGGTAAAATGATCGGTATGTGTTCAATTCATTGCCACCAAAATTTTAAGTTTACGAATTTGTGAAATTTTGTTGTCTGTAGATTTTCATTTCATTTTCTCGTCCTGTCTGTGTCTGGTTAGGATGGAATGTAAGAATTGGAAAAGGGAGGCATCAGGATTTGTAATCATATTTACATTTTTGCATTCCATTAGAATGACGAAAAAACATCTTCCCCCATGCCTTAGAGGATAAAAATTAACGACTTTCAAGGATTGAGAGTCAAGGAATGAAATTTGCATCTTGTTGCACTGAAAAACTTGGGCCACACCCATCAATTAGACCGTGTTTATCCTTCTTCCGTTCTACCCTTTTCTCATCTCGTCGAACCATTTTCCTTATCGCATAGCAGCAATATTTGATTTTCCTTTCGTCTTAACCTAAGATATTAGCTATCAAAATTGTCTAGGTATCTATTCATTTTACAGGAGTGGTATAAAGCTTCTTCAATGTATGAATCTCAGGTCCTTCGCTGGTCTTTAGCCACGATTTAGACTATTGGATCACCACTTGACCCATTAATATCTCTGAACTTTTTGAAGTTCGTCTTTTGTTCTCACTAAGGTGGATGAAAGTGCCATTTTGCAAAAGTCAACCTGGACTTATCAACTAGACAAGAAGCTTGAAATTCTTCACTAACGACAGAAATTATTTTCATACTGATTCACTGGCACTATATTTCTTTCTTTCCTCAATCTTAAGGAGGTTTGTTTGTTCAGCTTTTTAGTTTGATTGAGAGTGATTACTAGTTTGCCTAACTTGTCTGGTCTATTGCATTTGGAGAACACTTGAACTATTCCAGATATGGATCTTTGTAGAAATCTGTCAGTTCAACACAATTTGTTGGTGTTGTTTTAGTGATTAGTTTACTTCAACTATGCAGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGATCGAGAATGATGCTCCAGTACCGAAGCACATTCCTTTGCACCGACCAGGCCCTCTCCCTGAAGAGTTTTACAAGACGCTGGAGGCCATTAGTGGTGATAGTACTAAAAAAGTAGAGACACCCGAGAAAGCATCTCAGGTGACAGAATGACCTCTTTTTCATCATTTGAAGGATTCGAGTTTCGGATCCCGTTCTTTGTTTTTGGCTTTGATGTTATCTATTTTTGCTTGTTCCCCGATAGAGTGCCTGTATGCAGGGATTGTTCACAAAGTCATGTGTTTCAATAATCTCTATCCAATGTTGGAATTACATAAATTCACTTTTTCATTGCCTTGTTTACTTTTTACCGTGGACATCAAACCCCGTCATGGTTGGACTTTAGAATGAGCAATATTAAATTTAATTTTCCTACCATCTAAATACAAACTCTA

mRNA sequence

TGGCCCCTCCATCCTCCTGAAGCGACTGGCTCGTTACCACTGAGAAGCGTGATCAAGAGGAAGAGACAAGAGTTTTCCGACGATGTTCCTGCGAGCGATCGGACGGCCATTATTGGCCAAAGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTTGTTCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAGTAAAACCCTAAAGGAGATCCAGGCCGTCCCCGAGGACGAAGGATACCGTAAGGCGGTTGAGAGCTTTACGCGGCACCGCTTGAAGGTCTGCCAGGAAGAAGAGGATTGGGAGAATATCGAGAAGCGGCTTGGCTGTGGTCAGGTCGAGGAGCTAATCGAGGAGGCCCAGGACGAGCTCAAACTCATTGGTAAAATGATCGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGATCGAGAATGATGCTCCAGTACCGAAGCACATTCCTTTGCACCGACCAGGCCCTCTCCCTGAAGAGTTTTACAAGACGCTGGAGGCCATTAGTGGTGATAGTACTAAAAAAGTAGAGACACCCGAGAAAGCATCTCAGGTGACAGAATGACCTCTTTTTCATCATTTGAAGGATTCGAGTTTCGGATCCCGTTCTTTGTTTTTGGCTTTGATGTTATCTATTTTTGCTTGTTCCCCGATAGAGTGCCTGTATGCAGGGATTGTTCACAAAGTCATGTGTTTCAATAATCTCTATCCAATGTTGGAATTACATAAATTCACTTTTTCATTGCCTTGTTTACTTTTTACCGTGGACATCAAACCCCGTCATGGTTGGACTTTAGAATGAGCAATATTAAATTTAATTTTCCTACCATCTAAATACAAACTCTA

Coding sequence (CDS)

ATGTTCCTGCGAGCGATCGGACGGCCATTATTGGCCAAAGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTTGTTCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAGTAAAACCCTAAAGGAGATCCAGGCCGTCCCCGAGGACGAAGGATACCGTAAGGCGGTTGAGAGCTTTACGCGGCACCGCTTGAAGGTCTGCCAGGAAGAAGAGGATTGGGAGAATATCGAGAAGCGGCTTGGCTGTGGTCAGGTCGAGGAGCTAATCGAGGAGGCCCAGGACGAGCTCAAACTCATTGGTAAAATGATCGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGATCGAGAATGATGCTCCAGTACCGAAGCACATTCCTTTGCACCGACCAGGCCCTCTCCCTGAAGAGTTTTACAAGACGCTGGAGGCCATTAGTGGTGATAGTACTAAAAAAGTAGAGACACCCGAGAAAGCATCTCAGGTGACAGAATGA

Protein sequence

MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE*

Homology

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BLAST of Cucsa.108250.1 vs. Swiss-Prot
Match: NDUA5_ARATH (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Arabidopsis thaliana GN=At5g52840 PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.8e-76
Identity = 139/160 (86.88%), Postives = 146/160 (91.25%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAIGRPLLAKVKQTTGIVGLDVVPNAR VLI LYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TR RL VC+EEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETP 161
           ENDAP+PKH+P HRPGPLPE+FYKTLE +  +S  K E P
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEQFYKTLEGLIAES--KTEIP 158

[Close]

BLAST of Cucsa.108250.1 vs. Swiss-Prot
Match: NDUA5_DICDI (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyostelium discoideum GN=ndufa5 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.0e-18
Identity = 45/107 (42.06%), Postives = 71/107 (66.36%), Query Frame = 1


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K++TGI GL V PNAR +L GLY++ LK+++ +P   GYRK+ E  T+ RL V + E D
Sbjct: 6   IKKSTGITGLKVEPNARHILAGLYNQLLKKLEILPPTAGYRKSAELETKFRLGVIENETD 65

Query: 74  WENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 121
              IE ++  GQ+EELI +A+++LK++  + E   W +PD  +  V+
Sbjct: 66  IVKIENKIYAGQIEELIVQAKNDLKVVDLVHESRAWELPDKNKPPVV 112

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BLAST of Cucsa.108250.1 vs. Swiss-Prot
Match: NDUA5_BOVIN (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus GN=NDUFA5 PE=1 SV=3)

HSP 1 Score: 82.8 bits (203), Expect = 3.9e-15
Identity = 41/96 (42.71%), Postives = 61/96 (63.54%), Query Frame = 1


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY+K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPD 64

Query: 74  WENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPW 110
            + +E+RL  GQ+EE+I +A++EL L  KMI+W PW
Sbjct: 65  VKKLEERLQGGQIEEVILQAENELSLARKMIQWKPW 100

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BLAST of Cucsa.108250.1 vs. Swiss-Prot
Match: NDUA5_MOUSE (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Mus musculus GN=Ndufa5 PE=1 SV=3)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 40/96 (41.67%), Postives = 58/96 (60.42%), Query Frame = 1


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY+KTL  ++  P+   YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCDTPHERLTILYTKTLDILKHFPKHAAYRKYTEQITNEKLDMVKAEPD 64

Query: 74  WENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPW 110
            + +E  L  G+VEE+I +A+ EL L  KM++W PW
Sbjct: 65  VKKLEALLQGGEVEEVILQAEKELSLARKMLKWKPW 100

[Close]

BLAST of Cucsa.108250.1 vs. Swiss-Prot
Match: NDUA5_MACFA (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Macaca fascicularis GN=NDUFA5 PE=3 SV=3)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 39/96 (40.62%), Postives = 58/96 (60.42%), Query Frame = 1


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY+K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCSTPHERLSILYTKILDVLAEIPKNAAYRKYTEQITNEKLAMVKAEPD 64

Query: 74  WENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPW 110
            + +E +L  GQ+EE+I +A+ EL L  KM +W PW
Sbjct: 65  VKKLEDQLQGGQLEEVILQAEHELSLARKMRDWKPW 100

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BLAST of Cucsa.108250.1 vs. TrEMBL
Match: A0A0A0L623_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G184070 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.2e-79
Identity = 151/168 (89.88%), Postives = 153/168 (91.07%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWENIEKRLGCGQ+  LIE             +WDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQLFSLIE-------------KWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 169
           ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE
Sbjct: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 155

[Close]

BLAST of Cucsa.108250.1 vs. TrEMBL
Match: A0A061G198_THECC (NADH-ubiquinone oxidoreductase-related OS=Theobroma cacao GN=TCM_015272 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 5.6e-77
Identity = 142/161 (88.20%), Postives = 149/161 (92.55%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR I RPLLAKVK+TTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRLISRPLLAKVKETTGIVGLDVVPNAREVLIGLYTKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE DWE IEKRLGCGQVEELIEEA+DEL LIGKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEGDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPE 162
           ENDAPVPKH+PLHRPGPLPEEFY+TLEA+S     KV + E
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYQTLEAVSKKDAPKVTSGE 161

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BLAST of Cucsa.108250.1 vs. TrEMBL
Match: V4MNG3_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10026409mg PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 5.6e-77
Identity = 143/164 (87.20%), Postives = 149/164 (90.85%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGLDVVPNAR VLI LYSKTLKEIQ VPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNARTVLIDLYSKTLKEIQVVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEAQDEL L+GKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEAIEKRLGCGQVEELIEEAQDELTLVGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKAS 165
           ENDAP+PKH+P HRPGPLPEEFYKTL+AI+ +S  K E P   S
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEEFYKTLQAINSES--KTEIPAARS 162

[Close]

BLAST of Cucsa.108250.1 vs. TrEMBL
Match: A0A0B0PIL0_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_06511 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 7.4e-77
Identity = 141/157 (89.81%), Postives = 147/157 (93.63%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR I RPLLAKVK+TTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRMISRPLLAKVKETTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKV 158
           ENDAP+PKH+P HRPGPLPEEFYKTLEA+S     KV
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEEFYKTLEALSKKDEPKV 157

[Close]

BLAST of Cucsa.108250.1 vs. TrEMBL
Match: A0A0D2QJJ0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G013400 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 7.4e-77
Identity = 141/157 (89.81%), Postives = 147/157 (93.63%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR I RPLLAKVK+TTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRMISRPLLAKVKETTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKV 158
           ENDAP+PKH+P HRPGPLPEEFYKTLEA+S     KV
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEEFYKTLEALSKKDEPKV 157

[Close]

BLAST of Cucsa.108250.1 vs. TAIR10
Match: AT5G52840.1 (AT5G52840.1 NADH-ubiquinone oxidoreductase-related)

HSP 1 Score: 286.6 bits (732), Expect = 1.0e-77
Identity = 139/160 (86.88%), Postives = 146/160 (91.25%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAIGRPLLAKVKQTTGIVGLDVVPNAR VLI LYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TR RL VC+EEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETP 161
           ENDAP+PKH+P HRPGPLPE+FYKTLE +  +S  K E P
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEQFYKTLEGLIAES--KTEIP 158

[Close]

BLAST of Cucsa.108250.1 vs. TAIR10
Match: AT4G28005.1 (AT4G28005.1 BEST Arabidopsis thaliana protein match is: NADH-ubiquinone oxidoreductase-related (TAIR:AT5G52840.1))

HSP 1 Score: 104.4 bits (259), Expect = 7.1e-23
Identity = 52/93 (55.91%), Postives = 66/93 (70.97%), Query Frame = 1


Query: 65  LKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVIENDA 124
           ++VC EEEDW  IEKRL CG+VEELIEEA DEL L+GKMI              +I   A
Sbjct: 21  VEVCPEEEDWGVIEKRLACGKVEELIEEASDELTLVGKMI--------GSLHPNLIL--A 80

Query: 125 PVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKV 158
           P+PKH+P HRPGPLPEEFY+TL+A++ +S  ++
Sbjct: 81  PIPKHVPHHRPGPLPEEFYRTLQAVNSESKTEI 103

HSP 2 Score: 39.7 bits (91), Expect = 2.2e-03
Identity = 19/21 (90.48%), Postives = 19/21 (90.48%), Query Frame = 1


Query: 1  MFLRAIGRPLLAKVKQTTGIV 22
          MF R IGRPLLAKVKQTTGIV
Sbjct: 1  MFQRVIGRPLLAKVKQTTGIV 21

[Close]

BLAST of Cucsa.108250.1 vs. NCBI nr
Match: gi|449445977|ref|XP_004140748.1| (PREDICTED: probable NADH dehydrogenase [ubiquinone])

HSP 1 Score: 344.7 bits (883), Expect = 8.9e-92
Identity = 168/168 (100.00%), Postives = 168/168 (100.00%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 169
           ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE
Sbjct: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 168

[Close]

BLAST of Cucsa.108250.1 vs. NCBI nr
Match: gi|659077626|ref|XP_008439300.1| (PREDICTED: probable NADH dehydrogenase [ubiquinone])

HSP 1 Score: 337.8 bits (865), Expect = 1.1e-89
Identity = 164/168 (97.62%), Postives = 166/168 (98.81%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLI KMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAISGDST+KVET EKASQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISGDSTRKVETTEKASQVTE 168

[Close]

BLAST of Cucsa.108250.1 vs. NCBI nr
Match: gi|700202292|gb|KGN57425.1| (hypothetical protein Csa_3G184070 [Cucumis sativus])

HSP 1 Score: 303.9 bits (777), Expect = 1.7e-79
Identity = 151/168 (89.88%), Postives = 153/168 (91.07%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWENIEKRLGCGQ+  LIE             +WDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQLFSLIE-------------KWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 169
           ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE
Sbjct: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 155

[Close]

BLAST of Cucsa.108250.1 vs. NCBI nr
Match: gi|1009123008|ref|XP_015878313.1| (PREDICTED: probable NADH dehydrogenase [ubiquinone])

HSP 1 Score: 296.2 bits (757), Expect = 3.6e-77
Identity = 143/168 (85.12%), Postives = 152/168 (90.48%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRAI RPLLAKVKQTTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVE+F
Sbjct: 1   MFLRAIARPLLAKVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVENF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           T+HRLKVCQEEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMIEW+PWGVPDDYECEVI
Sbjct: 61  TQHRLKVCQEEEDWEAIEKRLGCGQVEELIEEARDELTLIGKMIEWEPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEA++  S +        SQ  E
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEALTSSSKEAPAVTSGESQSKE 168

[Close]

BLAST of Cucsa.108250.1 vs. NCBI nr
Match: gi|567218752|ref|XP_006413005.1| (hypothetical protein EUTSA_v10026409mg [Eutrema salsugineum])

HSP 1 Score: 295.0 bits (754), Expect = 8.1e-77
Identity = 143/164 (87.20%), Postives = 149/164 (90.85%), Query Frame = 1


Query: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGLDVVPNAR VLI LYSKTLKEIQ VPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNARTVLIDLYSKTLKEIQVVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEAQDEL L+GKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEAIEKRLGCGQVEELIEEAQDELTLVGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKAS 165
           ENDAP+PKH+P HRPGPLPEEFYKTL+AI+ +S  K E P   S
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEEFYKTLQAINSES--KTEIPAARS 162

The following BLAST results are available for this feature:

BLAST of Cucsa.108250.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NDUA5_ARATH	1.8e-76	86.88	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
NDUA5_DICDI	5.0e-18	42.06	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyos...	[more]
NDUA5_BOVIN	3.9e-15	42.71	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus GN=ND...	[more]
NDUA5_MOUSE	1.3e-13	41.67	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Mus musculus GN=...	[more]
NDUA5_MACFA	2.2e-13	40.63	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Macaca fascicula...	[more]

BLAST of Cucsa.108250.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L623_CUCSA	1.2e-79	89.88	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G184070 PE=4 SV=1	[more]
A0A061G198_THECC	5.6e-77	88.20	NADH-ubiquinone oxidoreductase-related OS=Theobroma cacao GN=TCM_015272 PE=4 SV=...	[more]
V4MNG3_EUTSA	5.6e-77	87.20	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10026409mg PE=4 SV=1	[more]
A0A0B0PIL0_GOSAR	7.4e-77	89.81	Uncharacterized protein OS=Gossypium arboreum GN=F383_06511 PE=4 SV=1	[more]
A0A0D2QJJ0_GOSRA	7.4e-77	89.81	Uncharacterized protein OS=Gossypium raimondii GN=B456_007G013400 PE=4 SV=1	[more]

BLAST of Cucsa.108250.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G52840.1	1.0e-77	86.88	NADH-ubiquinone oxidoreductase-related	[more]
AT4G28005.1	7.1e-23	55.91	BEST Arabidopsis thaliana protein match is: NADH-ubiquinone oxidored...	[more]

BLAST of Cucsa.108250.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445977\|ref\|XP_004140748.1\|	8.9e-92	100.00	PREDICTED: probable NADH dehydrogenase [ubiquinone]	[more]
gi\|659077626\|ref\|XP_008439300.1\|	1.1e-89	97.62	PREDICTED: probable NADH dehydrogenase [ubiquinone]	[more]
gi\|700202292\|gb\|KGN57425.1\|	1.7e-79	89.88	hypothetical protein Csa_3G184070 [Cucumis sativus]	[more]
gi\|1009123008\|ref\|XP_015878313.1\|	3.6e-77	85.12	PREDICTED: probable NADH dehydrogenase [ubiquinone]	[more]
gi\|567218752\|ref\|XP_006413005.1\|	8.1e-77	87.20	hypothetical protein EUTSA_v10026409mg [Eutrema salsugineum]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006806	ETC_CI_29_9

Vocabulary: **Molecular Function**
Term	Definition
GO:0016651	oxidoreductase activity, acting on NAD(P)H

Vocabulary: **Cellular Component**
Term	Definition
GO:0005743	mitochondrial inner membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0022904	respiratory electron transport chain

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022904	respiratory electron transport chain
biological_process	GO:0006120	mitochondrial electron transport, NADH to ubiquinone
biological_process	GO:0015992	proton transport
biological_process	GO:0006814	sodium ion transport
biological_process	GO:0006744	ubiquinone biosynthetic process
cellular_component	GO:0005743	mitochondrial inner membrane
molecular_function	GO:0016651	oxidoreductase activity, acting on NAD(P)H
molecular_function	GO:0008137	NADH dehydrogenase (ubiquinone) activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.108250	Cucsa.108250	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.108250.1	Cucsa.108250.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.108250.1.five_prime_UTR.1	Cucsa.108250.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.108250.1.CDS.1	Cucsa.108250.1.CDS.1	CDS
Cucsa.108250.1.CDS.2	Cucsa.108250.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.108250.1.three_prime_UTR.1	Cucsa.108250.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006806	ETC complex I subunit	PFAM	PF04716	ETC_C1_NDUFA5	coord: 22..88 score: 2.2
None	No IPR available	PANTHER	PTHR12653	NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT	coord: 1..143 score: 6.0