Cucsa.053580.3 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.053580.3
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Actin-depolymerizing factor
Location	scaffold00581 : 602964 .. 605705 (-)
Sequence length	1777

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGGGAAATTGAGAGGATTGGCTAAGTCACTGGCTCTAACTCCCTTTCTCTTTCTCTTCTTTCTATATCTTCTGTCTTACCCTCCGATTCTTGCCTACCACTTCTGACCTTTCTCTTATTCCATCCTTCTTTCTCTTCTTTTTCCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCATTTTCTCAACTTTTACTCATCCCCTTTCTCACACCCACCTCTTCTCCGATCATCATGTCGTTCCGAGGCCTCCGACGGGTATCCTTCTTTATGTTCTTCCTTTTATCTTTTCTTTACTTTAAACTCAACTGGGTATCACCTTTTTTCAATTGGGGTTTCTGGGTTTTCTTCCAATTTAATCCTAATCATGTTTATCTCAATATTCTTAACCCCTGCTTCAAGTGAATCCTGCATTTTTCTGTATTTTGTGACCTTAATTTCTTTCTCTCCCCTCTTCTGTGATGGGGTTTAGTTTTGTTTCCTCAAGAATGGTGTTTTTTGTTTTCTTTCCTTTTTTTTCTCTTTGAGGCATAGTTCAAAAGATTAAAAGAGTACTGTGTATTCAGATGAAAAAAGTTGGACTAGAAAGATCCTGTCTTTGATTTAAGGTTCTTGTTTTAGCTCAGAAAGCTTTGATTTAAAGGGAAGAAGTCATGATCCTTACTGATCAGGTTTTGGTGGTGAAGGGTGGGGTCTAGTAAGTATATGGGTTATGATTTTTTGCCAGTCTGTTGATGGACTTCTGTCTCCTTTGAGTTCTATGATTTTTTAAGGAAAATTTCAACTCAAGATTATAAAATTAGAATCTATTGGGGTTCTCAGGATTAATCATGATGTTCTGCTTCTTAGTCAAATTCAACTTTTGGTAACAAGAAGAGAACTCAGTAATTGAATAATGTTATAGATCTATCTCATGGTTTAATTTTTAAGGGCTTCTTCTGCTGCTCTATTTTGTTGAGGATTCTTACTTTGTTGGCATCATTTCCTTTGAAATCTACTGATTTGGAAATTTCTACTCTTACCGTTAATTTTTTATGCTTCTTTCATGGATCTCTGCTTGGTTGTTTGACATTAATGAATTTACCTTTGTAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACATTTTCTGAACTACAAAGAAAGAAAATGTATCGGTATGTGATCTTTAGGGTTGATGAAAAGAAAAGAGAGGTGGTGGTTGACAAGATTGGCAATCCTGCAGAGAGCTATGAAGATTTCACTGCTGCTCTGCCCGACAATGATTGCCGCTACGCTGTTTATGATTTTGATTTTGTGACATCCGACAATTGTCAGAAGAGCAAGATATTTTTCATAGCATGGTTAGCTTTCACCATCATTATACATCATGAACCTAATCTTATTTTTCTTACTGCTATATTAATGTCTATTGATCTGTATTATTCATTGGGTTGGCCCCATACAAAATTTTGGTCCATACTAAGGTAAACATTAGTTATCCCAAGGTCATAAGATTTTGATTCTATGATGACTATCATTTCTAAGCTCAGCCTCTAGGTTTTTCATGTTTCTAGTCAATTGCTCATTCATTGAACTGAAAAGCACTCATAAAGTGCTTCTTAGGCTTAGAACTTGGTTAATTTTATTGTGATATGTGTCGTTGATGCTTTTCTTTGACCTTCCTAAGGATATTTTCGTTATTTTTTGCTCTAGTTTGCCCATTCCAGTCTAAACTTTCTGTTCCTACAAATTTAACAAAGAAATGCATCAAATAACAAAATTTAACAGTAAAATCAATTAAGCCTAAGAAAAGACTGTTTTAGTGCTTTTAAGGAACTCATCTGGTGTACTTGCTAATTTCACTAAATACCTTCTTCGGGTGCATATGCTTTACTCTCCACACTGCAGAAGTTGGAGTTTAGTGGTTAGTTTAGTGGTTAGAGCTCTATATGCATCTTCTAATCTCGCTAAAGATCATAGTACATCAAAAGTTTTGGGCAAAACTTGAAGATCTTGTCATATTTTGTCAGAATAATTCTCCTTTTTGTTGATGATCTAGTGCACTCGTCGAATATCTTATTCTGAGAACCATTTAAGTTTTGACCTTGATTAGAAACAACAGGAATAGTTTTAATCATGTCATACCAATGATTATCGTGGGGTATCTTTTCTCTCTTGATCTCACACTACATTGTTGTACTTCAGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCAACATCCAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAAATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATCAGAGACCGTGCTCAATGAACATTCTCTGTTTGTTTAATTTGTTGTTCCCTTGGGGGTAAGCTGCCTTGTATTTTGGTTACTTATGAATTGGTTTATCCCTGCTTCAATATGTGTAAAACCGATCGTTGGAAAATGAAACCATTGCACAAAACCGTAAAATAAACTAACATCTGCAGAATGCTGATATATGGTGGATCGACATGATGTTGCTGTCTGGTTGCTATGAATGCTTCTCAGATGAAGATAAGTTTGTAATAGAAAATGCCTTAGAATCTATCAAACCTTATTATCATTGGAAATTTGTTCTATGATGGATCTGCCCCAATGTCTGGTTTTTTTCATTATGCCTTTAGAATTTGCACTTTATGATGTGAAATGAAGAACACGACTCGGTGTACCTCGGACATGGCA

mRNA sequence

GTTGGGAAATTGAGAGGATTGGCTAAGTCACTGGCTCTAACTCCCTTTCTCTTTCTCTTCTTTCTATATCTTCTGTCTTACCCTCCGATTCTTGCCTACCACTTCTGACCTTTCTCTTATTCCATCcttctttctcttctttttccttttcttcttcttcttcttcttcttcttcattttctCAACTTTTACTCATCCCCTTTCTCACACCCACCTCTTCTCCGATCATCATGTCGTTCCGAGGCCTCCGACGGGTATCCTTCTTTATGTTCTTCCTTTTATCTTTTCTTTACTTTAAACTCAACTGGGTATCACCTTTTTTCAATTGGGGTTTCTGGGTTTTCTTCCAATTTAATCCTAATCATGTTTATCTCAATATTCTTAACCCCTGCTTCAAGTGAATCCTGCATTTTTCTGTATTTTGTGACCTTAATTTCTTTCTCTCCCCTCTTCTGTGATGGGGTTTAGTTTTGTTTCCTCAAGAATGGTGTTTTTTGTTTTCTTTCCTTTTTTTTCTCTTTGAGGCATAGTTCAAAAGATTAAAAGAGTACTGTGTATTCAGATGAAAAAAGTTGGACTAGAAAGATCCTGTCTTTGATTTAAGGTTCTTGTTTTAGCTCAGAAAGCTTTGATTTAAAGGGAAGAAGTCATGATCCTTACTGATCAGGTTTTGGTGGTGAAGGGTGGGGTCTAGTAAGTATATGGGTTATGATTTTTTGCCAGTCTGTTGATGGACTTCTGTCTCCTTTGAGTTCTATGATTTTTTAAGGAAAATTTCAACTCAAGATTATAAAATTAGAATCTATTGGGGTTCTCAGGATTAATCATGATGTTCTGCTTCTTAGTCAAATTCAACTTTTGGTAACAAGAAGAGAACTCAGTAATTGAATAATGTTATAGATCTATCTCATGGTTTAATTTTTAAGGGCTTCTTCTGCTGCTCTATTTTGTTGAGGATTCTTACTTTGTTGGCATCATTTCCTTTGAAATCTACTGATTTGGAAATTTCTACTCTTACCGTTAATTTTTTATGCTTCTTTCATGGATCTCTGCTTGGTTGTTTGACATTAATGAATTTACCTTTGTAGCAAAACACTTTATCTGCAATGGGTGTGAATGAACACACCAAGAAAACATTTTCTGAACTACAAAGAAAGAAAATGTATCGGTATGTGATCTTTAGGGTTGATGAAAAGAAAAGAGAGGTGGTGGTTGACAAGATTGGCAATCCTGCAGAGAGCTATGAAGATTTCACTGCTGCTCTGCCCGACAATGATTGCCGCTACGCTGTTTATGATTTTGATTTTGTGACATCCGACAATTGTCAGAAGAGCAAGATATTTTTCATAGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCAACATCCAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAAATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATCAGAGACCGTGCTCAATGAACATTCTCTGTTTGTTTAATTTGTTGTTCCCTTGGGGAATGCTGATATATGGTGGATCGACATGATGTTGCTGTCTGGTTGCTATGAATGCTTCTCAGATGAAGATAAGTTTGTAATAGAAAATGCCTTAGAATCTATCAAACCTTATTATCATTGGAAATTTGTTCTATGATGGATCTGCCCCAATGTCTGGTTTTTTTCATTATGCCTTTAGAATTTGCACTTTATGATGTGAAATGAAGAACACGACTCGGTGTACCTCGGACATGGCA

Coding sequence (CDS)

ATGGGTGTGAATGAACACACCAAGAAAACATTTTCTGAACTACAAAGAAAGAAAATGTATCGGTATGTGATCTTTAGGGTTGATGAAAAGAAAAGAGAGGTGGTGGTTGACAAGATTGGCAATCCTGCAGAGAGCTATGAAGATTTCACTGCTGCTCTGCCCGACAATGATTGCCGCTACGCTGTTTATGATTTTGATTTTGTGACATCCGACAATTGTCAGAAGAGCAAGATATTTTTCATAGCATGGTCTCCAGCTAGCTCACGAATCCGTGCTAAGATGCTCTATGCAACATCCAAAGACAACTTTCGGCACGAGCTTGATGGCATCCACTATGAAATTCAAGCTACTGACCCTGCAGAGATGGACCTGGAAGTGATCAGAGACCGTGCTCAATGA

Protein sequence

MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRYAVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEMDLEVIRDRAQ*

Homology

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BLAST of Cucsa.053580.3 vs. Swiss-Prot
Match: ADF_VITVI (Actin-depolymerizing factor OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-57
Identity = 103/131 (78.63%), Postives = 121/131 (92.37%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV +H+K TF EL+RKK++RYVIF++DEKK+EVVV+K G PAES+++F AALP+NDCRY
Sbjct: 12  MGVADHSKNTFLELKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPENDCRY 71

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP SSRIRAKMLYATSK+ FR ELDG+HYEIQATDP 
Sbjct: 72  AVYDFDFVTSENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQATDPT 131

Query: 121 EMDLEVIRDRA 132
           EMDLEV+R+RA
Sbjct: 132 EMDLEVLRERA 142

[Close]

BLAST of Cucsa.053580.3 vs. Swiss-Prot
Match: ADF6_ARATH (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana GN=ADF6 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 3.8e-53
Identity = 96/131 (73.28%), Postives = 114/131 (87.02%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV + +K TF ELQRKK +RYV+F++DE K+EVVV+K GNP ESY+DF A+LPDNDCRY
Sbjct: 15  MGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    EL GIHYEIQATDP 
Sbjct: 75  AVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPT 134

Query: 121 EMDLEVIRDRA 132
           E+DLEV+R+RA
Sbjct: 135 EVDLEVLRERA 145

[Close]

BLAST of Cucsa.053580.3 vs. Swiss-Prot
Match: ADF11_ORYSJ (Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica GN=ADF11 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.0e-50
Identity = 89/131 (67.94%), Postives = 115/131 (87.79%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           +GV    K+TF ELQRKK +RYVIF++D+K +EVVV+K G+  ES++DF  +LP++DCRY
Sbjct: 14  IGVAAECKQTFLELQRKKSHRYVIFKIDDKCKEVVVEKTGSSTESFDDFMDSLPESDCRY 73

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           A+YDFDFVT +NCQKSKIFF+AWSP+ SRIRAKMLYATSK+ FR ELDG+HYEIQATDP+
Sbjct: 74  AIYDFDFVTEENCQKSKIFFVAWSPSVSRIRAKMLYATSKERFRRELDGVHYEIQATDPS 133

Query: 121 EMDLEVIRDRA 132
           E+D+E++R+RA
Sbjct: 134 ELDIELLRERA 144

[Close]

BLAST of Cucsa.053580.3 vs. Swiss-Prot
Match: ADF2_ORYSJ (Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica GN=ADF2 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 5.6e-49
Identity = 90/131 (68.70%), Postives = 111/131 (84.73%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV    + TF ELQ KK +RYVIF+++EK+++VVV+K G   ESY+DF A+LP+NDCRY
Sbjct: 14  MGVAPDIRDTFLELQMKKAFRYVIFKIEEKQKQVVVEKTGATTESYDDFLASLPENDCRY 73

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           A+YDFDFVT +N QKSKIFFIAWSP++SRIRAKMLY+TSKD  + ELDG HYEIQATDP 
Sbjct: 74  ALYDFDFVTGENVQKSKIFFIAWSPSTSRIRAKMLYSTSKDRIKQELDGFHYEIQATDPT 133

Query: 121 EMDLEVIRDRA 132
           E+DLEV+R+RA
Sbjct: 134 EVDLEVLRERA 144

[Close]

BLAST of Cucsa.053580.3 vs. Swiss-Prot
Match: ADF10_ORYSJ (Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica GN=ADF10 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 8.1e-48
Identity = 85/129 (65.89%), Postives = 111/129 (86.05%), Query Frame = 1


Query: 3   VNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRYAV 62
           V E +K  F EL+R+K++RYVIF++D+++ E+VV+K G P ESY+DFTA+LP +DCRYAV
Sbjct: 20  VPEKSKSAFWELKRRKVHRYVIFKIDDRREEIVVEKTGAPGESYDDFTASLPADDCRYAV 79

Query: 63  YDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPAEM 122
           YD DFV+ DNC+KSKIFFI+WSP+ SRIRAK +YA S++ FRHELDG+H+EIQATDP +M
Sbjct: 80  YDLDFVSDDNCRKSKIFFISWSPSVSRIRAKTIYAVSRNQFRHELDGVHFEIQATDPDDM 139

Query: 123 DLEVIRDRA 132
           DLEV+R RA
Sbjct: 140 DLEVLRGRA 148

[Close]

BLAST of Cucsa.053580.3 vs. TrEMBL
Match: A0A0A0L7Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G389830 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 5.2e-70
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY
Sbjct: 15  MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA
Sbjct: 75  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 134

Query: 121 EMDLEVIRDRAQ 133
           EMDLEVIRDRAQ
Sbjct: 135 EMDLEVIRDRAQ 146

[Close]

BLAST of Cucsa.053580.3 vs. TrEMBL
Match: M5VQ33_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012988mg PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-58
Identity = 110/131 (83.97%), Postives = 124/131 (94.66%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV++ +K TF ELQRKK++RYVIF+VDEKKREVVV+KIG PAESY+DF AALP+NDCRY
Sbjct: 15  MGVSDDSKNTFMELQRKKVHRYVIFKVDEKKREVVVEKIGGPAESYDDFAAALPENDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP++SRIRAKMLYATSKD FR ELDGIHYEIQATDP+
Sbjct: 75  AVYDFDFVTSENCQKSKIFFIAWSPSTSRIRAKMLYATSKDRFRRELDGIHYEIQATDPS 134

Query: 121 EMDLEVIRDRA 132
           EMDLEV+RDRA
Sbjct: 135 EMDLEVLRDRA 145

[Close]

BLAST of Cucsa.053580.3 vs. TrEMBL
Match: A0A067F314_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031876mg PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 4.6e-58
Identity = 109/132 (82.58%), Postives = 123/132 (93.18%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV +H+K T+ ELQRKK++RYVIF++DEKK+EVVV+K G PAESY+DFTA+LPDNDCRY
Sbjct: 20  MGVADHSKSTYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPDNDCRY 79

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP  SRIRAKMLYATSKD FR ELDGIHYEIQATDP+
Sbjct: 80  AVYDFDFVTSENCQKSKIFFIAWSPDVSRIRAKMLYATSKDRFRRELDGIHYEIQATDPS 139

Query: 121 EMDLEVIRDRAQ 133
           EMDLEVIRDRA+
Sbjct: 140 EMDLEVIRDRAR 151

[Close]

BLAST of Cucsa.053580.3 vs. TrEMBL
Match: V4S974_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006173mg PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 4.6e-58
Identity = 109/132 (82.58%), Postives = 123/132 (93.18%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV +H+K T+ ELQRKK++RYVIF++DEKK+EVVV+K G PAESY+DFTA+LPDNDCRY
Sbjct: 12  MGVADHSKSTYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPDNDCRY 71

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP  SRIRAKMLYATSKD FR ELDGIHYEIQATDP+
Sbjct: 72  AVYDFDFVTSENCQKSKIFFIAWSPDVSRIRAKMLYATSKDRFRRELDGIHYEIQATDPS 131

Query: 121 EMDLEVIRDRAQ 133
           EMDLEVIRDRA+
Sbjct: 132 EMDLEVIRDRAR 143

[Close]

BLAST of Cucsa.053580.3 vs. TrEMBL
Match: A0A067EUN7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031876mg PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 4.6e-58
Identity = 109/132 (82.58%), Postives = 123/132 (93.18%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV +H+K T+ ELQRKK++RYVIF++DEKK+EVVV+K G PAESY+DFTA+LPDNDCRY
Sbjct: 12  MGVADHSKSTYLELQRKKVHRYVIFKIDEKKKEVVVEKTGGPAESYDDFTASLPDNDCRY 71

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP  SRIRAKMLYATSKD FR ELDGIHYEIQATDP+
Sbjct: 72  AVYDFDFVTSENCQKSKIFFIAWSPDVSRIRAKMLYATSKDRFRRELDGIHYEIQATDPS 131

Query: 121 EMDLEVIRDRAQ 133
           EMDLEVIRDRA+
Sbjct: 132 EMDLEVIRDRAR 143

[Close]

BLAST of Cucsa.053580.3 vs. TAIR10
Match: AT2G31200.1 (AT2G31200.1 actin depolymerizing factor 6)

HSP 1 Score: 208.8 bits (530), Expect = 2.1e-54
Identity = 96/131 (73.28%), Postives = 114/131 (87.02%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV + +K TF ELQRKK +RYV+F++DE K+EVVV+K GNP ESY+DF A+LPDNDCRY
Sbjct: 15  MGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFF AWSP++S IRAK+LY+TSKD    EL GIHYEIQATDP 
Sbjct: 75  AVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPT 134

Query: 121 EMDLEVIRDRA 132
           E+DLEV+R+RA
Sbjct: 135 EVDLEVLRERA 145

[Close]

BLAST of Cucsa.053580.3 vs. TAIR10
Match: AT4G00680.1 (AT4G00680.1 actin depolymerizing factor 8)

HSP 1 Score: 184.5 bits (467), Expect = 4.3e-47
Identity = 78/130 (60.00%), Postives = 110/130 (84.62%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           M VN+  K  F EL+ K+ YR+++F++DEK ++V ++K+GNP E+Y+DFT+++PD++CRY
Sbjct: 8   MHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPDDECRY 67

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDF T DNCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ E++GI  E+QATDP+
Sbjct: 68  AVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVELQATDPS 127

Query: 121 EMDLEVIRDR 131
           EM L++I+ R
Sbjct: 128 EMSLDIIKGR 137

[Close]

BLAST of Cucsa.053580.3 vs. TAIR10
Match: AT1G01750.1 (AT1G01750.1 actin depolymerizing factor 11)

HSP 1 Score: 183.3 bits (464), Expect = 9.5e-47
Identity = 78/130 (60.00%), Postives = 110/130 (84.62%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           M V++  K  F EL+ K+ YR+++F++DEK ++V++DK+GNP E+YEDFT ++P+++CRY
Sbjct: 8   MHVSDECKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPEDECRY 67

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYD+DF T +NCQKSKIFFIAWSP +SR+R+KMLYA+SKD F+ ELDGI  E+QATDP+
Sbjct: 68  AVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPS 127

Query: 121 EMDLEVIRDR 131
           EM L++I+ R
Sbjct: 128 EMSLDIIKGR 137

[Close]

BLAST of Cucsa.053580.3 vs. TAIR10
Match: AT4G25590.1 (AT4G25590.1 actin depolymerizing factor 7)

HSP 1 Score: 178.3 bits (451), Expect = 3.1e-45
Identity = 81/131 (61.83%), Postives = 107/131 (81.68%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           M V +  K  F EL+ K+ YR++IFR+D   ++VVV+K+GNP E+Y+DFTA+LP N+CRY
Sbjct: 8   MAVEDECKLKFLELKSKRNYRFIIFRIDG--QQVVVEKLGNPDETYDDFTASLPANECRY 67

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AV+DFDF+T +NCQKSKIFFIAWSP SSR+R KM+YA+SKD F+ ELDGI  E+QATDP+
Sbjct: 68  AVFDFDFITDENCQKSKIFFIAWSPDSSRVRMKMVYASSKDRFKRELDGIQVELQATDPS 127

Query: 121 EMDLEVIRDRA 132
           EM  ++I+ RA
Sbjct: 128 EMSFDIIKSRA 136

[Close]

BLAST of Cucsa.053580.3 vs. TAIR10
Match: AT2G16700.1 (AT2G16700.1 actin depolymerizing factor 5)

HSP 1 Score: 176.0 bits (445), Expect = 1.5e-44
Identity = 78/132 (59.09%), Postives = 105/132 (79.55%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           M V +    +F +++ KK++RY++F+++EK R+V VDK+G   ESY D   +LP +DCRY
Sbjct: 12  MRVTDECTSSFMDMKWKKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDLEDSLPVDDCRY 71

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AV+DFDFVT DNC+KSKIFFIAWSP +S+IRAK+LYATSKD  R  L+GIHYE+QATDP 
Sbjct: 72  AVFDFDFVTVDNCRKSKIFFIAWSPEASKIRAKILYATSKDGLRRVLEGIHYELQATDPT 131

Query: 121 EMDLEVIRDRAQ 133
           EM  ++I+DRA+
Sbjct: 132 EMGFDIIQDRAK 143

[Close]

BLAST of Cucsa.053580.3 vs. NCBI nr
Match: gi|449468548|ref|XP_004151983.1| (PREDICTED: actin-depolymerizing factor [Cucumis sativus])

HSP 1 Score: 271.6 bits (693), Expect = 7.5e-70
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY
Sbjct: 15  MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA
Sbjct: 75  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 134

Query: 121 EMDLEVIRDRAQ 133
           EMDLEVIRDRAQ
Sbjct: 135 EMDLEVIRDRAQ 146

[Close]

BLAST of Cucsa.053580.3 vs. NCBI nr
Match: gi|659093222|ref|XP_008447430.1| (PREDICTED: actin-depolymerizing factor [Cucumis melo])

HSP 1 Score: 270.4 bits (690), Expect = 1.7e-69
Identity = 131/132 (99.24%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGVNEHTKKTFSELQRKKMYRYVIF+VDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY
Sbjct: 15  MGVNEHTKKTFSELQRKKMYRYVIFKVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA
Sbjct: 75  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 134

Query: 121 EMDLEVIRDRAQ 133
           EMDLEVIRDRAQ
Sbjct: 135 EMDLEVIRDRAQ 146

[Close]

BLAST of Cucsa.053580.3 vs. NCBI nr
Match: gi|645260128|ref|XP_008235687.1| (PREDICTED: actin-depolymerizing factor [Prunus mume])

HSP 1 Score: 235.3 bits (599), Expect = 6.0e-59
Identity = 111/131 (84.73%), Postives = 125/131 (95.42%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV++ +KKTF ELQRKK++RYVIF+VDEKKREVVV+KIG PAESY+DF AALP+NDCRY
Sbjct: 15  MGVSDDSKKTFMELQRKKVHRYVIFKVDEKKREVVVEKIGGPAESYDDFVAALPENDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP++SRIRAKMLYATSKD FR ELDGIHYEIQATDP+
Sbjct: 75  AVYDFDFVTSENCQKSKIFFIAWSPSTSRIRAKMLYATSKDRFRRELDGIHYEIQATDPS 134

Query: 121 EMDLEVIRDRA 132
           EMDLEV+RDRA
Sbjct: 135 EMDLEVLRDRA 145

[Close]

BLAST of Cucsa.053580.3 vs. NCBI nr
Match: gi|694387478|ref|XP_009369488.1| (PREDICTED: actin-depolymerizing factor [Pyrus x bretschneideri])

HSP 1 Score: 234.2 bits (596), Expect = 1.3e-58
Identity = 110/131 (83.97%), Postives = 123/131 (93.89%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV++ +K TF ELQRKK++RYVIF+VDEKKREVVV+KIG PAESY+DF AALPDNDCRY
Sbjct: 15  MGVSDESKNTFMELQRKKLHRYVIFKVDEKKREVVVEKIGGPAESYDDFVAALPDNDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP+++RIRAKMLYATSKD FR ELDGIHYEIQATDP 
Sbjct: 75  AVYDFDFVTSENCQKSKIFFIAWSPSTARIRAKMLYATSKDRFRRELDGIHYEIQATDPT 134

Query: 121 EMDLEVIRDRA 132
           EMDLEV+RDRA
Sbjct: 135 EMDLEVLRDRA 145

[Close]

BLAST of Cucsa.053580.3 vs. NCBI nr
Match: gi|595801654|ref|XP_007201857.1| (hypothetical protein PRUPE_ppa012988mg [Prunus persica])

HSP 1 Score: 233.4 bits (594), Expect = 2.3e-58
Identity = 110/131 (83.97%), Postives = 124/131 (94.66%), Query Frame = 1


Query: 1   MGVNEHTKKTFSELQRKKMYRYVIFRVDEKKREVVVDKIGNPAESYEDFTAALPDNDCRY 60
           MGV++ +K TF ELQRKK++RYVIF+VDEKKREVVV+KIG PAESY+DF AALP+NDCRY
Sbjct: 15  MGVSDDSKNTFMELQRKKVHRYVIFKVDEKKREVVVEKIGGPAESYDDFAAALPENDCRY 74

Query: 61  AVYDFDFVTSDNCQKSKIFFIAWSPASSRIRAKMLYATSKDNFRHELDGIHYEIQATDPA 120
           AVYDFDFVTS+NCQKSKIFFIAWSP++SRIRAKMLYATSKD FR ELDGIHYEIQATDP+
Sbjct: 75  AVYDFDFVTSENCQKSKIFFIAWSPSTSRIRAKMLYATSKDRFRRELDGIHYEIQATDPS 134

Query: 121 EMDLEVIRDRA 132
           EMDLEV+RDRA
Sbjct: 135 EMDLEVLRDRA 145

The following BLAST results are available for this feature:

BLAST of Cucsa.053580.3 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ADF_VITVI	1.5e-57	78.63	Actin-depolymerizing factor OS=Vitis vinifera PE=2 SV=1	[more]
ADF6_ARATH	3.8e-53	73.28	Actin-depolymerizing factor 6 OS=Arabidopsis thaliana GN=ADF6 PE=1 SV=1	[more]
ADF11_ORYSJ	3.0e-50	67.94	Actin-depolymerizing factor 11 OS=Oryza sativa subsp. japonica GN=ADF11 PE=2 SV=...	[more]
ADF2_ORYSJ	5.6e-49	68.70	Actin-depolymerizing factor 2 OS=Oryza sativa subsp. japonica GN=ADF2 PE=2 SV=1	[more]
ADF10_ORYSJ	8.1e-48	65.89	Actin-depolymerizing factor 10 OS=Oryza sativa subsp. japonica GN=ADF10 PE=2 SV=...	[more]

BLAST of Cucsa.053580.3 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7Z2_CUCSA	5.2e-70	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G389830 PE=3 SV=1	[more]
M5VQ33_PRUPE	1.6e-58	83.97	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012988mg PE=3 SV=1	[more]
A0A067F314_CITSI	4.6e-58	82.58	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031876mg PE=3 SV=1	[more]
V4S974_9ROSI	4.6e-58	82.58	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006173mg PE=3 SV=1	[more]
A0A067EUN7_CITSI	4.6e-58	82.58	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031876mg PE=3 SV=1	[more]

BLAST of Cucsa.053580.3 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G31200.1	2.1e-54	73.28	actin depolymerizing factor 6	[more]
AT4G00680.1	4.3e-47	60.00	actin depolymerizing factor 8	[more]
AT1G01750.1	9.5e-47	60.00	actin depolymerizing factor 11	[more]
AT4G25590.1	3.1e-45	61.83	actin depolymerizing factor 7	[more]
AT2G16700.1	1.5e-44	59.09	actin depolymerizing factor 5	[more]

BLAST of Cucsa.053580.3 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449468548\|ref\|XP_004151983.1\|	7.5e-70	100.00	PREDICTED: actin-depolymerizing factor [Cucumis sativus]	[more]
gi\|659093222\|ref\|XP_008447430.1\|	1.7e-69	99.24	PREDICTED: actin-depolymerizing factor [Cucumis melo]	[more]
gi\|645260128\|ref\|XP_008235687.1\|	6.0e-59	84.73	PREDICTED: actin-depolymerizing factor [Prunus mume]	[more]
gi\|694387478\|ref\|XP_009369488.1\|	1.3e-58	83.97	PREDICTED: actin-depolymerizing factor [Pyrus x bretschneideri]	[more]
gi\|595801654\|ref\|XP_007201857.1\|	2.3e-58	83.97	hypothetical protein PRUPE_ppa012988mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002108	ADF-H
IPR017904	ADF/Cofilin

Vocabulary: **Molecular Function**
Term	Definition
GO:0003779	actin binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular
GO:0015629	actin cytoskeleton

Vocabulary: **Biological Process**
Term	Definition
GO:0030042	actin filament depolymerization

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
cellular_component	GO:0005622	intracellular
molecular_function	GO:0003779	actin binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.053580	Cucsa.053580	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.053580.3	Cucsa.053580.3-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.053580.3.three_prime_UTR.2	Cucsa.053580.3.three_prime_UTR.2	three_prime_UTR
Cucsa.053580.3.three_prime_UTR.1	Cucsa.053580.3.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.053580.3.CDS.2	Cucsa.053580.3.CDS.2	CDS
Cucsa.053580.3.CDS.1	Cucsa.053580.3.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.053580.3.five_prime_UTR.1	Cucsa.053580.3.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 8..129 score: 4.3
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 5..132 score: 2.9
IPR002108	Actin-depolymerising factor homology domain	PROFILE	PS51263	ADF_H	coord: 1..132 score: 39
IPR017904	ADF/Cofilin/Destrin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 1..132 score: 8.5
None	No IPR available	PANTHER	PTHR11913:SF20	ACTIN-DEPOLYMERIZING FACTOR 6	coord: 1..132 score: 8.5
None	No IPR available	unknown	SSF55753	Actin depolymerizing proteins	coord: 2..130 score: 1.31