Csa6G446280.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa6G446280.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Phenylalanine ammonia-lyase; contains IPR001106 (Aromatic amino acid lyase), IPR008948 (L-Aspartase-like), IPR024083 (Fumarase/histidase, N-terminal)
Location	Chr6 : 20855215 .. 20855835 (-)
Sequence length	501

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGATTCTAATTCCAATAACTTACAAGAATTTTTTCATTCTTCGTTTTCTTATTCTTTTGGATTTCATTCCATTTCTTTCTTTCATACTTACAAATGGCACCAATTGAGACCACATGCAAGAGCTTCACTCATGATCCTCTCAATTGGGGTGTGGCTGCGGAGTCCCTCAAGGGGAGCCATCTAGATGAGGTCAAACGCATGGTAGAGGAGTACCGAAGGCCCCTTGTGAAGCTTGGTGGTGAAACTCTCACCATTTCTCAAGTAGCTGCTATTGCTACTCGAGATTTTGATGTTACTGTTGAGCTTTCTGAGTCTGCTAGAGCAGGTGTGAAAGCTAGTAGTGATTGGGTTATGGAGAGTATGAAAAAAGGCACTGATAGTTACGGAGTTACTACTGGTTTTGGTGCTACCTCTCACAGAAGGACAAAAAATGGTGGGGCTCTTCAGAAGGAGCTTATTCGATTCTTGAATGCTGGTATCTTTGGAAATGGTGCTGAGTCATGTCATACTTTGTCTCGTTCGGCTACCAGAGCAGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGTTACTCAGGTATTAGATTTGAGATTTTGGAAGCCATCACCAAACTCTTGA

mRNA sequence

ATGGCACCAATTGAGACCACATGCAAGAGCTTCACTCATGATCCTCTCAATTGGGGTGTGGCTGCGGAGTCCCTCAAGGGGAGCCATCTAGATGAGGTCAAACGCATGGTAGAGGAGTACCGAAGGCCCCTTGTGAAGCTTGGTGGTGAAACTCTCACCATTTCTCAAGTAGCTGCTATTGCTACTCGAGATTTTGATGTTACTGTTGAGCTTTCTGAGTCTGCTAGAGCAGGTGTGAAAGCTAGTAGTGATTGGGTTATGGAGAGTATGAAAAAAGGCACTGATAGTTACGGAGTTACTACTGGTTTTGGTGCTACCTCTCACAGAAGGACAAAAAATGGTGGGGCTCTTCAGAAGGAGCTTATTCGATTCTTGAATGCTGGTATCTTTGGAAATGGTGCTGAGTCATGTCATACTTTGTCTCGTTCGGCTACCAGAGCAGCCATGTTGGTTACTCAGGTATTAGATTTGAGATTTTGGAAGCCATCACCAAACTCTTGA

Coding sequence (CDS)

ATGGCACCAATTGAGACCACATGCAAGAGCTTCACTCATGATCCTCTCAATTGGGGTGTGGCTGCGGAGTCCCTCAAGGGGAGCCATCTAGATGAGGTCAAACGCATGGTAGAGGAGTACCGAAGGCCCCTTGTGAAGCTTGGTGGTGAAACTCTCACCATTTCTCAAGTAGCTGCTATTGCTACTCGAGATTTTGATGTTACTGTTGAGCTTTCTGAGTCTGCTAGAGCAGGTGTGAAAGCTAGTAGTGATTGGGTTATGGAGAGTATGAAAAAAGGCACTGATAGTTACGGAGTTACTACTGGTTTTGGTGCTACCTCTCACAGAAGGACAAAAAATGGTGGGGCTCTTCAGAAGGAGCTTATTCGATTCTTGAATGCTGGTATCTTTGGAAATGGTGCTGAGTCATGTCATACTTTGTCTCGTTCGGCTACCAGAGCAGCCATGTTGGTTACTCAGGTATTAGATTTGAGATTTTGGAAGCCATCACCAAACTCTTGA

Protein sequence

MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS*

Homology

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BLAST of Csa6G446280.1 vs. Swiss-Prot
Match: PAL2_POPKI (Phenylalanine ammonia-lyase G2B OS=Populus kitakamiensis GN=PALG2B PE=3 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 5.0e-63
Identity = 121/140 (86.43%), Postives = 125/140 (89.29%), Query Frame = 1


Query: 12  THDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVEL 71
           T+DPLNWG+AAESLKGSHLDEVKRM+EEYR P+VKLGGETLTI QV AIA+RD  V VEL
Sbjct: 18  TNDPLNWGMAAESLKGSHLDEVKRMIEEYRNPVVKLGGETLTIGQVTAIASRDVGVMVEL 77

Query: 72  SESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFG 131
           SE ARAGVKASSDWVM+SM KGTDSYGVTTGFGATSHRRTK GG LQKELIRFLNAGIFG
Sbjct: 78  SEEARAGVKASSDWVMDSMSKGTDSYGVTTGFGATSHRRTKQGGELQKELIRFLNAGIFG 137

Query: 132 NGAESCHTLSRSATRAAMLV 152
           NG ES HTL RSATRAAMLV
Sbjct: 138 NGTESSHTLPRSATRAAMLV 157

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BLAST of Csa6G446280.1 vs. Swiss-Prot
Match: PAL1_PEA (Phenylalanine ammonia-lyase 1 OS=Pisum sativum GN=PAL1 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 8.6e-63
Identity = 121/138 (87.68%), Postives = 125/138 (90.58%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNWGVAAE++KGSHLDEVKRMVEEYR+P+V+LGGETLTISQVAAIA  D  V VELSE
Sbjct: 33  DPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHDHGVKVELSE 92

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
           SARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIRFLNAGIFGNG
Sbjct: 93  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 152

Query: 134 AESCHTLSRSATRAAMLV 152
            ES HTL  +ATRAAMLV
Sbjct: 153 TESSHTLPHTATRAAMLV 170

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BLAST of Csa6G446280.1 vs. Swiss-Prot
Match: PALY_CAMSI (Phenylalanine ammonia-lyase OS=Camellia sinensis GN=PAL PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 3.3e-62
Identity = 119/138 (86.23%), Postives = 126/138 (91.30%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNWGVAAE++KGSHL+EVK MVEE+R+P+V+LGGETLTISQVAAIA R  +V VELSE
Sbjct: 23  DPLNWGVAAEAMKGSHLEEVKGMVEEFRKPVVRLGGETLTISQVAAIAVRGSEVAVELSE 82

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
           SAR GVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIRFLNAGIFGNG
Sbjct: 83  SAREGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKEGGALQKELIRFLNAGIFGNG 142

Query: 134 AESCHTLSRSATRAAMLV 152
            ESCHTL +SATRAAMLV
Sbjct: 143 TESCHTLPQSATRAAMLV 160

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BLAST of Csa6G446280.1 vs. Swiss-Prot
Match: PALY_TRISU (Phenylalanine ammonia-lyase OS=Trifolium subterraneum GN=PAL1 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.6e-61
Identity = 121/138 (87.68%), Postives = 125/138 (90.58%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNWGVAAE++KGSHLDEVKRMVEEYR+P+V+LGGETLTISQVAAIA  D   TVELSE
Sbjct: 35  DPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHD-GATVELSE 94

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
           SARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIRFLNAGIFGNG
Sbjct: 95  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 154

Query: 134 AESCHTLSRSATRAAMLV 152
            ES HTL  +ATRAAMLV
Sbjct: 155 TESNHTLPHTATRAAMLV 171

[Close]

BLAST of Csa6G446280.1 vs. Swiss-Prot
Match: PAL2_PEA (Phenylalanine ammonia-lyase 2 OS=Pisum sativum GN=PAL2 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.0e-60
Identity = 118/138 (85.51%), Postives = 124/138 (89.86%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNWGVAAE++KGSHLDEVKRMV+EYR+P+V+LGGETLTISQVAAIA  D  V VELSE
Sbjct: 34  DPLNWGVAAEAMKGSHLDEVKRMVDEYRKPVVRLGGETLTISQVAAIAAHDHGVKVELSE 93

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
           SARAGVKASSDWVMESM KGTDSYGVTT  GATSHRRTK GGALQKELIRFLNAGIFGNG
Sbjct: 94  SARAGVKASSDWVMESMNKGTDSYGVTTVHGATSHRRTKQGGALQKELIRFLNAGIFGNG 153

Query: 134 AESCHTLSRSATRAAMLV 152
           +ES HTL  +ATRAAMLV
Sbjct: 154 SESTHTLPHTATRAAMLV 171

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BLAST of Csa6G446280.1 vs. TrEMBL
Match: A0A0A0KK77_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G446280 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 3.1e-88
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 1


Query: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60
           MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI
Sbjct: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60

Query: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120
           ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE
Sbjct: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120

Query: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS 167
           LIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS
Sbjct: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS 166

[Close]

BLAST of Csa6G446280.1 vs. TrEMBL
Match: A0A0A0KH72_CUCSA (Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_6G445770 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 1.9e-64
Identity = 129/148 (87.16%), Postives = 136/148 (91.89%), Query Frame = 1


Query: 4   IETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATR 63
           +E+ C S   DPLNWGVAAE+LKGSHLDEVKRMV+EYRRP+VKLGGETLTISQVAAIATR
Sbjct: 16  MESFCTSHVVDPLNWGVAAEALKGSHLDEVKRMVDEYRRPVVKLGGETLTISQVAAIATR 75

Query: 64  DFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIR 123
           D DV V+L+ESARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIR
Sbjct: 76  DNDVVVKLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 135

Query: 124 FLNAGIFGNGAESCHTLSRSATRAAMLV 152
           FLNAGIFGNGAES HTL  SATRAAMLV
Sbjct: 136 FLNAGIFGNGAESNHTLPHSATRAAMLV 163

[Close]

BLAST of Csa6G446280.1 vs. TrEMBL
Match: A0A0A0KF39_CUCSA (Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_6G445760 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 2.4e-64
Identity = 131/148 (88.51%), Postives = 137/148 (92.57%), Query Frame = 1


Query: 4   IETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATR 63
           +E+ C   ++DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATR
Sbjct: 13  VESLCIG-SNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATR 72

Query: 64  DFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIR 123
           D DV VELSES+RAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIR
Sbjct: 73  DTDVIVELSESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 132

Query: 124 FLNAGIFGNGAESCHTLSRSATRAAMLV 152
           FLNAGIFG+GAES HTL  SATRAAMLV
Sbjct: 133 FLNAGIFGSGAESDHTLPHSATRAAMLV 159

[Close]

BLAST of Csa6G446280.1 vs. TrEMBL
Match: A0A0A0KID7_CUCSA (Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_6G445750 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 7.1e-64
Identity = 131/141 (92.91%), Postives = 132/141 (93.62%), Query Frame = 1


Query: 12  TH-DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVE 71
           TH DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRD DV VE
Sbjct: 14  THSDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDSDVIVE 73

Query: 72  LSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIF 131
           LSESAR GVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIRFLNAGIF
Sbjct: 74  LSESARVGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIF 133

Query: 132 GNGAESCHTLSRSATRAAMLV 152
           G+GAES HTL  SATRAAMLV
Sbjct: 134 GSGAESDHTLPHSATRAAMLV 154

[Close]

BLAST of Csa6G446280.1 vs. TrEMBL
Match: A0A0A0KK73_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G445740 PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 9.2e-64
Identity = 132/156 (84.62%), Postives = 139/156 (89.10%), Query Frame = 1


Query: 1   MAPIETT------CKSFTH-DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLT 60
           MAPI+ T      C + TH DPLNWG+AA+SLKGSHLDEVKRMV+EYRRPLVKLGGETLT
Sbjct: 1   MAPIDQTNDDSSFCTNTTHPDPLNWGLAADSLKGSHLDEVKRMVQEYRRPLVKLGGETLT 60

Query: 61  ISQVAAIATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKN 120
           ISQVAAIATRD DV VELSESARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK 
Sbjct: 61  ISQVAAIATRDTDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQ 120

Query: 121 GGALQKELIRFLNAGIFGNGAESCHTLSRSATRAAM 150
           GGALQKELIRFLNAGIFG+GAES HTL  SATRAA+
Sbjct: 121 GGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAI 156

[Close]

BLAST of Csa6G446280.1 vs. TAIR10
Match: AT3G53260.1 (AT3G53260.1 phenylalanine ammonia-lyase 2)

HSP 1 Score: 226.9 bits (577), Expect = 9.4e-60
Identity = 113/138 (81.88%), Postives = 122/138 (88.41%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNWG+AA+ +KGSHLDEVK+MVEEYRRP+V LGGETLTI QVAAI+T    V VEL+E
Sbjct: 26  DPLNWGLAADQMKGSHLDEVKKMVEEYRRPVVNLGGETLTIGQVAAISTVGGSVKVELAE 85

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
           ++RAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTKNG ALQ ELIRFLNAGIFGN 
Sbjct: 86  TSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNT 145

Query: 134 AESCHTLSRSATRAAMLV 152
            E+CHTL +SATRAAMLV
Sbjct: 146 KETCHTLPQSATRAAMLV 163

[Close]

BLAST of Csa6G446280.1 vs. TAIR10
Match: AT2G37040.1 (AT2G37040.1 PHE ammonia lyase 1)

HSP 1 Score: 219.9 bits (559), Expect = 1.2e-57
Identity = 112/138 (81.16%), Postives = 118/138 (85.51%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNWG AAE +KGSHLDEVKRMV E+R+P+V LGGETLTI QVAAI+T    V VELSE
Sbjct: 34  DPLNWGAAAEQMKGSHLDEVKRMVAEFRKPVVNLGGETLTIGQVAAISTIGNSVKVELSE 93

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
           +ARAGV ASSDWVMESM KGTDSYGVTTGFGATSHRRTKNG ALQKELIRFLNAGIFG+ 
Sbjct: 94  TARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNAGIFGST 153

Query: 134 AESCHTLSRSATRAAMLV 152
            E+ HTL  SATRAAMLV
Sbjct: 154 KETSHTLPHSATRAAMLV 171

[Close]

BLAST of Csa6G446280.1 vs. TAIR10
Match: AT3G10340.1 (AT3G10340.1 phenylalanine ammonia-lyase 4)

HSP 1 Score: 208.4 bits (529), Expect = 3.5e-54
Identity = 107/139 (76.98%), Postives = 115/139 (82.73%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNW   AE+LKGSHLDEVKRMV+EYR+  VKLGGETLTI QVAA+A      TVEL+E
Sbjct: 16  DPLNWNATAEALKGSHLDEVKRMVKEYRKEAVKLGGETLTIGQVAAVARGGGGSTVELAE 75

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
            ARAGVKASS+WVMESM +GTDSYGVTTGFGATSHRRTK GGALQ ELIRFLNAGIFG G
Sbjct: 76  EARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPG 135

Query: 134 A-ESCHTLSRSATRAAMLV 152
           A ++ HTL +  TRAAMLV
Sbjct: 136 AGDTSHTLPKPTTRAAMLV 154

[Close]

BLAST of Csa6G446280.1 vs. TAIR10
Match: AT5G04230.2 (AT5G04230.2 phenyl alanine ammonia-lyase 3)

HSP 1 Score: 186.0 bits (471), Expect = 1.8e-47
Identity = 97/141 (68.79%), Postives = 112/141 (79.43%), Query Frame = 1


Query: 14  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDFDVTVELSE 73
           DPLNW VAAE+LKGSHL+EVK+MV++YR+  V+LGGETLTI QVAA+A+     TVELSE
Sbjct: 13  DPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVASGG--PTVELSE 72

Query: 74  SARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNG 133
            AR GVKASSDWVMESM + TD+YG+TTGFG++S RRT  G ALQKELIR+LNAGIF  G
Sbjct: 73  EARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFATG 132

Query: 134 AES---CHTLSRSATRAAMLV 152
            E     +TL R ATRAAML+
Sbjct: 133 NEDDDRSNTLPRPATRAAMLI 151

[Close]

BLAST of Csa6G446280.1 vs. NCBI nr
Match: gi|700192962|gb|KGN48166.1| (hypothetical protein Csa_6G446280 [Cucumis sativus])

HSP 1 Score: 332.4 bits (851), Expect = 4.5e-88
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 1


Query: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60
           MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI
Sbjct: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60

Query: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120
           ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE
Sbjct: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120

Query: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS 167
           LIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS
Sbjct: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLVTQVLDLRFWKPSPNS 166

[Close]

BLAST of Csa6G446280.1 vs. NCBI nr
Match: gi|778722345|ref|XP_011658467.1| (PREDICTED: phenylalanine ammonia-lyase 1-like [Cucumis sativus])

HSP 1 Score: 300.4 bits (768), Expect = 1.9e-78
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 1


Query: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60
           MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI
Sbjct: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60

Query: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120
           ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE
Sbjct: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120

Query: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLV 152
           LIRFLNAGIFGNGAESCHTLSRSATRAAMLV
Sbjct: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLV 151

[Close]

BLAST of Csa6G446280.1 vs. NCBI nr
Match: gi|659096694|ref|XP_008449236.1| (PREDICTED: phenylalanine ammonia-lyase-like [Cucumis melo])

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-74
Identity = 145/151 (96.03%), Postives = 145/151 (96.03%), Query Frame = 1


Query: 1   MAPIETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60
           MAPIET CKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI
Sbjct: 1   MAPIETICKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 60

Query: 61  ATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKE 120
           ATRD DVTVELSESARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKE
Sbjct: 61  ATRDIDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKE 120

Query: 121 LIRFLNAGIFGNGAESCHTLSRSATRAAMLV 152
           LIRFLNAGIFGNG ES HTLSRSATRAAMLV
Sbjct: 121 LIRFLNAGIFGNGVESSHTLSRSATRAAMLV 151

[Close]

BLAST of Csa6G446280.1 vs. NCBI nr
Match: gi|778722342|ref|XP_011658466.1| (PREDICTED: phenylalanine ammonia-lyase 1-like [Cucumis sativus])

HSP 1 Score: 256.5 bits (654), Expect = 3.1e-65
Identity = 139/168 (82.74%), Postives = 145/168 (86.31%), Query Frame = 1


Query: 1   MAPIETT------CKSFTH-DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLT 60
           MAPI+ T      C + TH DPLNWG+AA+SLKGSHLDEVKRMV+EYRRPLVKLGGETLT
Sbjct: 1   MAPIDQTNDDSSFCTNTTHPDPLNWGLAADSLKGSHLDEVKRMVQEYRRPLVKLGGETLT 60

Query: 61  ISQVAAIATRDFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKN 120
           ISQVAAIATRD DV VELSESARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK 
Sbjct: 61  ISQVAAIATRDTDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQ 120

Query: 121 GGALQKELIRFLNAGIFGNGAESCHTLSRSATRAAMLV-----TQVLD 157
           GGALQKELIRFLNAGIFG+GAES HTL  SATRAAMLV      QVLD
Sbjct: 121 GGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQVLD 168

[Close]

BLAST of Csa6G446280.1 vs. NCBI nr
Match: gi|659096696|ref|XP_008449237.1| (PREDICTED: phenylalanine ammonia-lyase-like [Cucumis melo])

HSP 1 Score: 253.8 bits (647), Expect = 2.0e-64
Identity = 129/148 (87.16%), Postives = 136/148 (91.89%), Query Frame = 1


Query: 4   IETTCKSFTHDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATR 63
           +E+ C S   DPLNWGVAAE+LKGSHLDEVKRMV+EYRRP+VKLGGETLTISQVAAIATR
Sbjct: 16  MESFCTSHVVDPLNWGVAAEALKGSHLDEVKRMVDEYRRPVVKLGGETLTISQVAAIATR 75

Query: 64  DFDVTVELSESARAGVKASSDWVMESMKKGTDSYGVTTGFGATSHRRTKNGGALQKELIR 123
           D DV V+L+ESARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRTK GGALQKELIR
Sbjct: 76  DSDVVVKLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 135

Query: 124 FLNAGIFGNGAESCHTLSRSATRAAMLV 152
           FLNAGIFGNGAES HTL  SATRAAMLV
Sbjct: 136 FLNAGIFGNGAESNHTLPHSATRAAMLV 163

The following BLAST results are available for this feature:

BLAST of Csa6G446280.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PAL2_POPKI	5.0e-63	86.43	Phenylalanine ammonia-lyase G2B OS=Populus kitakamiensis GN=PALG2B PE=3 SV=1	[more]
PAL1_PEA	8.6e-63	87.68	Phenylalanine ammonia-lyase 1 OS=Pisum sativum GN=PAL1 PE=2 SV=1	[more]
PALY_CAMSI	3.3e-62	86.23	Phenylalanine ammonia-lyase OS=Camellia sinensis GN=PAL PE=2 SV=1	[more]
PALY_TRISU	1.6e-61	87.68	Phenylalanine ammonia-lyase OS=Trifolium subterraneum GN=PAL1 PE=3 SV=1	[more]
PAL2_PEA	1.0e-60	85.51	Phenylalanine ammonia-lyase 2 OS=Pisum sativum GN=PAL2 PE=2 SV=1	[more]

BLAST of Csa6G446280.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK77_CUCSA	3.1e-88	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G446280 PE=4 SV=1	[more]
A0A0A0KH72_CUCSA	1.9e-64	87.16	Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_6G445770 PE=3 SV=1	[more]
A0A0A0KF39_CUCSA	2.4e-64	88.51	Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_6G445760 PE=3 SV=1	[more]
A0A0A0KID7_CUCSA	7.1e-64	92.91	Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_6G445750 PE=3 SV=1	[more]
A0A0A0KK73_CUCSA	9.2e-64	84.62	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G445740 PE=3 SV=1	[more]

BLAST of Csa6G446280.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G53260.1	9.4e-60	81.88	phenylalanine ammonia-lyase 2	[more]
AT2G37040.1	1.2e-57	81.16	PHE ammonia lyase 1	[more]
AT3G10340.1	3.5e-54	76.98	phenylalanine ammonia-lyase 4	[more]
AT5G04230.2	1.8e-47	68.79	phenyl alanine ammonia-lyase 3	[more]

BLAST of Csa6G446280.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700192962\|gb\|KGN48166.1\|	4.5e-88	100.00	hypothetical protein Csa_6G446280 [Cucumis sativus]	[more]
gi\|778722345\|ref\|XP_011658467.1\|	1.9e-78	100.00	PREDICTED: phenylalanine ammonia-lyase 1-like [Cucumis sativus]	[more]
gi\|659096694\|ref\|XP_008449236.1\|	3.7e-74	96.03	PREDICTED: phenylalanine ammonia-lyase-like [Cucumis melo]	[more]
gi\|778722342\|ref\|XP_011658466.1\|	3.1e-65	82.74	PREDICTED: phenylalanine ammonia-lyase 1-like [Cucumis sativus]	[more]
gi\|659096696\|ref\|XP_008449237.1\|	2.0e-64	87.16	PREDICTED: phenylalanine ammonia-lyase-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001106	Aromatic_Lyase
IPR008948	L-Aspartase-like
IPR024083	Fumarase/histidase_N

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009821	alkaloid biosynthetic process
biological_process	GO:0009800	cinnamic acid biosynthetic process
biological_process	GO:0006559	L-phenylalanine catabolic process
biological_process	GO:0006570	tyrosine metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0045548	phenylalanine ammonia-lyase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa6G446280	Csa6G446280	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa6G446280.1	Csa6G446280.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G446280.1.cds2	Csa6G446280.1.cds2	CDS
Csa6G446280.1.cds1	Csa6G446280.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G446280.1.utr5p1	Csa6G446280.1.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001106	Aromatic amino acid lyase	PFAM	PF00221	Lyase_aromatic	coord: 52..151 score: 2.3
IPR008948	L-Aspartase-like	unknown	SSF48557	L-aspartase-like	coord: 14..151 score: 4.71
IPR024083	Fumarase/histidase, N-terminal	GENE3D	G3DSA:1.10.275.10		coord: 13..151 score: 1.3
None	No IPR available	PANTHER	PTHR10362	HISTIDINE AMMONIA-LYASE	coord: 14..151 score: 1.2E
None	No IPR available	PANTHER	PTHR10362:SF17	SUBFAMILY NOT NAMED	coord: 14..151 score: 1.2E