Csa5G157280.1 (mRNA) Cucumber (Chinese Long) v2

NameCsa5G157280.1
TypemRNA
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionSubtilisin-like serine protease; contains IPR015500 (Peptidase S8, subtilisin-related), IPR023827 (Peptidase S8, subtilisin, Asp-active site)
LocationChr5 : 5539360 .. 5541564 (+)
Sequence length2205
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACTGAGCTTTGGAGTGCTTCCAACACCAATTTACAAACCTACATTGTTCATGTTAAGCAACCAGAGGTTGAGATTTTGGGTGACACCATTGATCTACAAAATTGGTACACATCATTCTTACCAGAAACGATCGAAGCGTCATCCAACGAACAATCACGGTTACTTTATTCATATCGACACGTGATCAGTGGTTTCTCTGCAAGACTTACAAAAGAACAAGTTAAAACAATGGAAGAGAAGGATGGTTTCATCTCAGCCATGCCTGAAACCACATTGAATTTGCACACAACTCACACACCTGAATATTTGGGGTTGAACCAACATTTTGGGTTATGGAAAAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGCCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAACAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATGTTTCGATTGGGAAATCACCAAATGACGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTTGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCAGTTGGAATGGCACCTTTAGCTCATATTGCAGTGTACAAAGTTTGTTCTCCAAAAGGTTGTTCCAGTAGTGATATACTTGCTGCCTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCTTCGACTCCATTTTTCAAAGACACAATTGCCGTAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCAGGGAATTCAGGCCCTTCCAAAAATACATTAGCCAACGAAGCACCATGGATTTTAACTGTTGGAGCTAGCACCATTGATAGAAAAATCGTGGCATTGGCAAAGCTTGAAAGTGGCAAAGTCTTTACCGGTGAATCTCTGTTCCAGCCAAGAGATTTCTCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGTTCTGAATATTGTGTTGAAGGTTCACTTGAAAAGCTGAATGTGACAGGAAAAATCGTGGTATGTGAACGAGGAGGAGGAATAGGCAGAATAGCAAAAGGGTTGGTTGTGAAAAATGGTGGCGGCGCTGCCATGATTCTTGTAAACCAAAAACCAGATGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACAACCCATTTAAGCTATGAAGATGGACTTAAAATCAAAGAATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTGAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCTATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACAGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAACACAAACACGAACACAAAATCGACTTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTAATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCGTGGATCAAGATTTGAAACCAGCGAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTTTATGACATTCAGCCCGATGATTATGTTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAGACAAGTAACGTGTTCGACGGTATCAAGAATACGAGAAGGCGATCTGAATTATCCTTCGTTTGCTGTGAGTTTGGGAGCTGATTCACAGGCGTTTAATAGAACAGTGACAAATGTGGGTGATGCTAATTCAGTTTATTACGCCATTGTTAAGGCACCAGCTGGAGTTTCAGTGAGAGTTACACCAAGAAATTTGAAGTTCTCAAAGTTGAATGAAAAATTGACATATTCTGTAACTTTCAGCCGGATCGACTTTGTTAGAACAAGAAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAATAAACACATAGTGAGAAGTCCAATCTCTGTAAAGCTCACCTAA

mRNA sequence

ATGAAAACTGAGCTTTGGAGTGCTTCCAACACCAATTTACAAACCTACATTGTTCATGTTAAGCAACCAGAGGTTGAGATTTTGGGTGACACCATTGATCTACAAAATTGGTACACATCATTCTTACCAGAAACGATCGAAGCGTCATCCAACGAACAATCACGGTTACTTTATTCATATCGACACGTGATCAGTGGTTTCTCTGCAAGACTTACAAAAGAACAAGTTAAAACAATGGAAGAGAAGGATGGTTTCATCTCAGCCATGCCTGAAACCACATTGAATTTGCACACAACTCACACACCTGAATATTTGGGGTTGAACCAACATTTTGGGTTATGGAAAAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGCCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAACAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATGTTTCGATTGGGAAATCACCAAATGACGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTTGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCAGTTGGAATGGCACCTTTAGCTCATATTGCAGTGTACAAAGTTTGTTCTCCAAAAGGTTGTTCCAGTAGTGATATACTTGCTGCCTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCTTCGACTCCATTTTTCAAAGACACAATTGCCGTAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCAGGGAATTCAGGCCCTTCCAAAAATACATTAGCCAACGAAGCACCATGGATTTTAACTGTTGGAGCTAGCACCATTGATAGAAAAATCGTGGCATTGGCAAAGCTTGAAAGTGGCAAAGTCTTTACCGGTGAATCTCTGTTCCAGCCAAGAGATTTCTCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGTTCTGAATATTGTGTTGAAGGTTCACTTGAAAAGCTGAATGTGACAGGAAAAATCGTGGTATGTGAACGAGGAGGAGGAATAGGCAGAATAGCAAAAGGGTTGGTTGTGAAAAATGGTGGCGGCGCTGCCATGATTCTTGTAAACCAAAAACCAGATGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACAACCCATTTAAGCTATGAAGATGGACTTAAAATCAAAGAATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTGAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCTATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACAGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAACACAAACACGAACACAAAATCGACTTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTAATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCGTGGATCAAGATTTGAAACCAGCGAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTTTATGACATTCAGCCCGATGATTATGTTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAGACAAGTAACGTGTTCGACGGTATCAAGAATACGAGAAGGCGATCTGAATTATCCTTCGTTTGCTGTGAGTTTGGGAGCTGATTCACAGGCGTTTAATAGAACAGTGACAAATGTGGGTGATGCTAATTCAGTTTATTACGCCATTGTTAAGGCACCAGCTGGAGTTTCAGTGAGAGTTACACCAAGAAATTTGAAGTTCTCAAAGTTGAATGAAAAATTGACATATTCTGTAACTTTCAGCCGGATCGACTTTGTTAGAACAAGAAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAATAAACACATAGTGAGAAGTCCAATCTCTGTAAAGCTCACCTAA

Coding sequence (CDS)

ATGAAAACTGAGCTTTGGAGTGCTTCCAACACCAATTTACAAACCTACATTGTTCATGTTAAGCAACCAGAGGTTGAGATTTTGGGTGACACCATTGATCTACAAAATTGGTACACATCATTCTTACCAGAAACGATCGAAGCGTCATCCAACGAACAATCACGGTTACTTTATTCATATCGACACGTGATCAGTGGTTTCTCTGCAAGACTTACAAAAGAACAAGTTAAAACAATGGAAGAGAAGGATGGTTTCATCTCAGCCATGCCTGAAACCACATTGAATTTGCACACAACTCACACACCTGAATATTTGGGGTTGAACCAACATTTTGGGTTATGGAAAAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGCCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAACAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATGTTTCGATTGGGAAATCACCAAATGACGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTTGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCAGTTGGAATGGCACCTTTAGCTCATATTGCAGTGTACAAAGTTTGTTCTCCAAAAGGTTGTTCCAGTAGTGATATACTTGCTGCCTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCTTCGACTCCATTTTTCAAAGACACAATTGCCGTAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCAGGGAATTCAGGCCCTTCCAAAAATACATTAGCCAACGAAGCACCATGGATTTTAACTGTTGGAGCTAGCACCATTGATAGAAAAATCGTGGCATTGGCAAAGCTTGAAAGTGGCAAAGTCTTTACCGGTGAATCTCTGTTCCAGCCAAGAGATTTCTCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGTTCTGAATATTGTGTTGAAGGTTCACTTGAAAAGCTGAATGTGACAGGAAAAATCGTGGTATGTGAACGAGGAGGAGGAATAGGCAGAATAGCAAAAGGGTTGGTTGTGAAAAATGGTGGCGGCGCTGCCATGATTCTTGTAAACCAAAAACCAGATGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACAACCCATTTAAGCTATGAAGATGGACTTAAAATCAAAGAATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTGAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCTATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACAGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAACACAAACACGAACACAAAATCGACTTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTAATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCGTGGATCAAGATTTGAAACCAGCGAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTTTATGACATTCAGCCCGATGATTATGTTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAGACAAGTAACGTGTTCGACGGTATCAAGAATACGAGAAGGCGATCTGAATTATCCTTCGTTTGCTGTGAGTTTGGGAGCTGATTCACAGGCGTTTAATAGAACAGTGACAAATGTGGGTGATGCTAATTCAGTTTATTACGCCATTGTTAAGGCACCAGCTGGAGTTTCAGTGAGAGTTACACCAAGAAATTTGAAGTTCTCAAAGTTGAATGAAAAATTGACATATTCTGTAACTTTCAGCCGGATCGACTTTGTTAGAACAAGAAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAATAAACACATAGTGAGAAGTCCAATCTCTGTAAAGCTCACCTAA

Protein sequence

MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISVKLT*
BLAST of Csa5G157280.1 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 6.0e-177
Identity = 347/748 (46.39%), Postives = 469/748 (62.70%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQ-NWYTSFLPETIEASSNEQSRLLYSYRHVIS 66
           S+S+++  TYIVH+ + ++     + DL  NWY S L      S ++ + LLY+Y + I 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMP---SSFDLHSNWYDSSL-----RSISDSAELLYTYENAIH 82

Query: 67  GFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFG-LWKNSNFGKGVI 126
           GFS RLT+E+  ++  + G IS +PE    LHTT TP +LGL++H   L+  +     V+
Sbjct: 83  GFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVV 142

Query: 127 IGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTFNLANNVSI 186
           +GVLDTG+ P   S++DEG    P+ WKG CE G    AS+CN KLIGAR F      ++
Sbjct: 143 VGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTM 202

Query: 187 G--------KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
           G        +SP D++GHGTHT+STAAG+ V+GA  LG A G A GMAP A +AVYKVC 
Sbjct: 203 GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW 262

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 306
             GC SSDILAA+D AI D V+VLS+SLG   + +++D +A+GAFAA+++GI VSCSAGN
Sbjct: 263 LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGN 322

Query: 307 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYA 366
           +GPS ++L+N APWI TVGA T+DR   ALA L +GK FTG SLF+      K LP +YA
Sbjct: 323 AGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA 382

Query: 367 GK-SGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPD 426
           G  S       C+ G+L    V GKIV+C+RG    R+ KG VVK  GG  MIL N   +
Sbjct: 383 GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAAN 442

Query: 427 GFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFS 486
           G   +A+AH+LP T +  + G  I+ Y+ +  NP ASIS  GT++G +    SP +A+FS
Sbjct: 443 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP---SPVVAAFS 502

Query: 487 SRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSG 546
           SRGP   +P ILKPD+  PGVNILAAW          + + +  FN+ISGTSMSCPH+SG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 547 IAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAA 606
           +AAL+KS HP WSPAAI+SA+MT+A      GKP++D    KP+  F  G+GHV+P+ A 
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 622

Query: 607 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSL-GA 666
           NPGL+YD+  +DY+ +LC L YT  Q+  + RR  TC         DLNYPSFAV++ G 
Sbjct: 623 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 682

Query: 667 DSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVR 726
            +  + RTVT+VG A +    +     GV + V P  L F + NEK +Y+VTF+ +D  +
Sbjct: 683 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSK 742

Query: 727 TRSEFSEGYLIWVSNKHIVRSPISVKLT 735
                S G + W   KH+V SP+++  T
Sbjct: 743 PSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Csa5G157280.1 vs. Swiss-Prot
Match: SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 3.9e-176
Identity = 351/754 (46.55%), Postives = 473/754 (62.73%), Query Frame = 1

Query: 14  QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETI----EASSNEQSRLLYSYRHVISGFSA 73
           QTYIV +  P  E         +W+ SFL E +    E      SRLLYSY   I GF+A
Sbjct: 26  QTYIVQL-HPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAA 85

Query: 74  RLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQ--HFGLWKNSNFGKGVIIGV 133
           +LT+ + + +      ++  P+  L + TT++ ++LGL+   + G+W  S FG+G IIGV
Sbjct: 86  QLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGV 145

Query: 134 LDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV--SIG 193
           LDTG+ P  PSF+D GMPS P KWKG C+    F +S CN KLIGAR F   + V  S  
Sbjct: 146 LDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPE 205

Query: 194 KSPN---------DENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 253
           +SPN         D  GHGTHTAST  G+ V  A  LGN  G A GMAP AHIAVYKVC 
Sbjct: 206 ESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCW 265

Query: 254 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 313
             GC SSDILAA+D AI D VDVLSLSLG    P + DTIA+G F A+++GI V C+AGN
Sbjct: 266 FNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGN 325

Query: 314 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDF--SSKFLPLV 373
           +GP ++++AN APW+ T+GA T+DR+  A+ +L +GK+  GESL+  +    + + + ++
Sbjct: 326 NGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVI 385

Query: 374 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 433
           Y    G +GSE+C+ GSL +  + GK+V+C+RG   GR  KG  VK  GG AMIL N + 
Sbjct: 386 YV-TGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 434 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 493
           +      + H+LP T + Y + + +K Y+N++  PKA I F GT++G    + +P +A F
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGR---SRAPEVAQF 505

Query: 494 SSRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLS 553
           S+RGP  A+P ILKPD+  PGVNI+AAWP  L        + +  F V+SGTSMSCPH+S
Sbjct: 506 SARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVS 565

Query: 554 GIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAA 613
           GI ALI+S +PNWSPAAIKSA+MT+AD+ + QGK I D + KPA  FA+G+GHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 614 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREG-DLNYPSFAV--SL 673
           NPGLVY+IQP DY+ YLC L +T + +  I  + V+C+ + R   G  LNYPS AV    
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685

Query: 674 GADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTF--SRI 733
           G  ++   R VTNVG  NS+Y   VKAP G+ V V P+ L F  +++ L+Y V F   + 
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745

BLAST of Csa5G157280.1 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.1e-170
Identity = 350/759 (46.11%), Postives = 456/759 (60.08%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISG 66
           S+S+  L++YIVHV++     L  + +  NW+ S L      SS + + LLYSY   + G
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLLRSL--PSSPQPATLLYSYSRAVHG 83

Query: 67  FSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 126
           FSARL+  Q   +      IS +P+    +HTTHTP +LG +Q+ GLW NSN+G+ VI+G
Sbjct: 84  FSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVG 143

Query: 127 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTF---------- 186
           VLDTGI P HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F          
Sbjct: 144 VLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNG 203

Query: 187 NLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
              +     +SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C 
Sbjct: 204 TKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICW 263

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPST--PFFKDTIAVGAFAAIKKGIFVSCSA 306
             GC  SDILAA+D A+ DGV V+SLS+GA  +   +  D+IA+GAF A + GI VSCSA
Sbjct: 264 TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSA 323

Query: 307 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 366
           GNSGP+  T  N APWILTVGAST+DR+  A A    GKVFTG SL+         L LV
Sbjct: 324 GNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLV 383

Query: 367 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 426
           Y+G     GS  C  G L    V GKIV+C+RGG   R+ KG  VK  GGA MIL N   
Sbjct: 384 YSGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAE 443

Query: 427 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 486
            G    A++H++P T +  + G +I++YI +S +P A ISF GTL+G   +  SP +A+F
Sbjct: 444 SGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG--PSPPSPRVAAF 503

Query: 487 SSRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTNTKSTFNVISGTSMSCP 546
           SSRGP   +P ILKPD+  PGVNILA W     P  L+ +     +  FN+ISGTSMSCP
Sbjct: 504 SSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR---RVQFNIISGTSMSCP 563

Query: 547 HLSGIAALIKSNHPNWSPAAIKSAIMTSA-DVRNPQGKPIVDQDL-KPANFFAMGSGHVN 606
           H+SG+AAL++  HP+WSPAAIKSA++T+A DV N  G+PI D    K +N F  G+GHV+
Sbjct: 564 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHVD 623

Query: 607 PSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVT----CSTVSRIREGDLNYPS 666
           P+KA NPGLVYDI+  +YV +LC +  +    ++  +  T    C T      GDLNYPS
Sbjct: 624 PNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPS 683

Query: 667 FAVSLGADSQA--FNRTVTNVG-DANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYS 726
           F+V   +  +   + R V NVG + ++VY   VK+PA V + V+P  L FSK    L Y 
Sbjct: 684 FSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYE 743

Query: 727 VTFSRIDF---VRTRSEFSEGYLIWVSNKHIVRSPISVK 733
           VTF  +     V +      G + W   +H+V+SP++V+
Sbjct: 744 VTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of Csa5G157280.1 vs. Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 7.4e-167
Identity = 339/754 (44.96%), Postives = 457/754 (60.61%), Query Frame = 1

Query: 5   LWSASNTNL--QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRH 64
           ++ ASN +   QTY++H      + +  ++     + S   E I         + Y Y +
Sbjct: 31  IFFASNVSSRKQTYVIHTVTTSTKHIVTSL-----FNSLQTENINDDDFSLPEIHYIYEN 90

Query: 65  VISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKG 124
            +SGFSA LT +Q+ T++   GFISA P+  L+LHTT++ E+LGL    GLW  ++    
Sbjct: 91  AMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSD 150

Query: 125 VIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV 184
           VIIG++DTGI P H SF D  M   P++W+G C+    F +S CN K+IGA  F      
Sbjct: 151 VIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYES 210

Query: 185 SIGK--------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKV 244
            +GK        S  D  GHGTHTASTAAG  V  A   G A+G A GM   + IA YK 
Sbjct: 211 IVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKA 270

Query: 245 CSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSA 304
           C   GC+S+D++AA+D AI DGVDV+SLSLG  S PF+ D IA+  F A++K IFVSCSA
Sbjct: 271 CWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSA 330

Query: 305 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 364
           GNSGP+ +T++N APW++TV AS  DR   A+ ++ + K   G SL++ +  S K LPL 
Sbjct: 331 GNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGK--SLKNLPLA 390

Query: 365 YAGKSGIE-GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQK 424
           +   +G E G+ +C+  SL++  V GKIV+C RG   GR AKG  VK  GGAAM+LV+ +
Sbjct: 391 FNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAKGEEVKRSGGAAMLLVSTE 450

Query: 425 PDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMAS 484
            +G   LA+ HVLP   L + DG  +  Y+  + N  AS+ F GT  G  A    P +A+
Sbjct: 451 AEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYGATA----PMVAA 510

Query: 485 FSSRGPCQASPGILKPDITGPGVNILAAW-PFPLNNNTNTNTKST-FNVISGTSMSCPHL 544
           FSSRGP  A P I KPDI  PG+NILA W PF   +   ++ +   FN+ISGTSM+CPH+
Sbjct: 511 FSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHI 570

Query: 545 SGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDL----KPANFFAMGSGHVN 604
           SGIAALIKS H +WSPA IKSAIMT+A + + + +PI D+        A  FA G+G+V+
Sbjct: 571 SGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVD 630

Query: 605 PSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTC-STVSRIREGDLNYPSFA 664
           P++A +PGLVYD    DY+ YLC L YT  ++ +      TC S    +  GDLNYPSFA
Sbjct: 631 PTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFA 690

Query: 665 VSL--GADSQA--FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSV 724
           V+L  GA+ +   + RTVTNVG     Y   V+ P GV VRV P+ LKF K  E+L+Y+V
Sbjct: 691 VNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTV 750

Query: 725 TFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISV 732
           T+   +  R  S  S G L+W+ +K+ VRSPI+V
Sbjct: 751 TYD-AEASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of Csa5G157280.1 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.8e-165
Identity = 335/743 (45.09%), Postives = 446/743 (60.03%), Query Frame = 1

Query: 11  TNLQTYIV---HVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGF 70
           T  +TYI+   H  +PE       +   +WYTS L        N +S LLY+Y     GF
Sbjct: 25  TAKKTYIIRVNHSDKPE-----SFLTHHDWYTSQL--------NSESSLLYTYTTSFHGF 84

Query: 71  SARLTKEQVKTM-EEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 130
           SA L   +  ++    +  +    +    LHTT TPE+LGLN  FG+    +   GVIIG
Sbjct: 85  SAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIG 144

Query: 131 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNVSIGK 190
           VLDTG+ P   SF+D  MP  P+KWKG CE    F + +CN KLIGAR+F+    ++ G 
Sbjct: 145 VLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGG 204

Query: 191 ---------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSP 250
                    SP D +GHGTHT++TAAG+ V+ A  LG A G A GMA  A +A YKVC  
Sbjct: 205 GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWS 264

Query: 251 KGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNS 310
            GC  SDILAA+D AI DGVDVLSLSLG  S P+++DTIA+GAF+A+++G+FVSCSAGNS
Sbjct: 265 TGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNS 324

Query: 311 GPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAG 370
           GP++ ++AN APW++TVGA T+DR   A A L +GK  TG SL+      +K L LVY  
Sbjct: 325 GPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-N 384

Query: 371 KSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGF 430
           K     S  C+ GSL+   V GKIVVC+RG    R+ KG VV++ GG  MI+ N    G 
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGE 444

Query: 431 STLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSR 490
             +A++H+LP   +  + G  ++EY+ S   P A + F+GT+L  +    SP +A+FSSR
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP---SPVVAAFSSR 504

Query: 491 GPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSGIA 550
           GP   +P ILKPD+ GPGVNILA W   +        + ++ FN++SGTSMSCPH+SG+A
Sbjct: 505 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 564

Query: 551 ALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAANP 610
            L+K+ HP WSP+AIKSA+MT+A V +    P+ D  D   +N +A GSGHV+P KA +P
Sbjct: 565 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSP 624

Query: 611 GLVYDIQPDDYVPYLCHL-YT-DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADS 670
           GLVYDI  ++Y+ +LC L YT D  V+I+ R  V CS       G LNYPSF+V  G   
Sbjct: 625 GLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK-KFSDPGQLNYPSFSVLFGGKR 684

Query: 671 QA-FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRT 730
              + R VTNVG A+SVY   V     V + V P  L F  + EK  Y+VTF     V  
Sbjct: 685 VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM 744

BLAST of Csa5G157280.1 vs. TrEMBL
Match: A0A0A0KMQ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1)

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 1

Query: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60
           MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY
Sbjct: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60

Query: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120
           RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG
Sbjct: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120

Query: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180
           KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180

Query: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240

Query: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300
           ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360
           ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360

Query: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420
           EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH
Sbjct: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420

Query: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480
           VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540

Query: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600
           WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660
           YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD
Sbjct: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660

Query: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720
           ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS
Sbjct: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720

Query: 721 NKHIVRSPISVKLT 735
           NKHIVRSPISVKLT
Sbjct: 721 NKHIVRSPISVKLT 734

BLAST of Csa5G157280.1 vs. TrEMBL
Match: A0A0A0KKE3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 1.2e-277
Identity = 490/729 (67.22%), Postives = 578/729 (79.29%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           +NL TYIVHVK+PEV       DL++W+ SFLP ++E +S EQ  LLYSYR+V+SGFSAR
Sbjct: 28  SNLHTYIVHVKKPEV-----VDDLESWHRSFLPTSLE-NSEEQPTLLYSYRNVMSGFSAR 87

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E VK MEEKDGF+SA  ET ++LHTTH+P +LGLN+ FG WK+SNFGKGVIIGVLD 
Sbjct: 88  LTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGVLDG 147

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGK------SP 190
           GI P+HPSF D GMP PPAKWKGRCEF  S CNNKLIGAR+ NLA+    GK      SP
Sbjct: 148 GITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGARSLNLASQALKGKITTLDDSP 207

Query: 191 NDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAA 250
            DE+GHGTHTASTAAGTFV GAEALGNA G AVGMAPLAH+A+YKVC  + CS+ DILA 
Sbjct: 208 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDILAG 267

Query: 251 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 310
           LDAA++DGVDVLS+SLG P  PFF D  A+GAFAAI+KGIFVSCSA NSGP   TL+NEA
Sbjct: 268 LDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLSNEA 327

Query: 311 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 370
           PWILTV ASTIDRKI A AKL +G+ F GESLFQP DF   FLPLV+ G+   E    C 
Sbjct: 328 PWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKN-ETVALCA 387

Query: 371 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 430
           EGSL+ ++V GK+VVC+RGGGI RIAKG+ VKN GGAAMIL+N + DGF+T A+AHVLP 
Sbjct: 388 EGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHVLPA 447

Query: 431 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 490
           +H+S+   LKIK YINS+  P A+I F+GT +G+    FSPA+A+FSSRGP  ASPGILK
Sbjct: 448 SHVSHTAALKIKAYINSTTYPTATIVFKGTTIGD---DFSPAIAAFSSRGPSLASPGILK 507

Query: 491 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 550
           PDITGPGV+ILAAWPFPL+N  NTNTKSTFN++SGTSMSCPHLSGIAALIKS HP+WSPA
Sbjct: 508 PDITGPGVSILAAWPFPLDN--NTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPA 567

Query: 551 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 610
           AIKS+IMT+A++ N +G PIVDQ L+PA+ FA+G+GHVNPSKA +PGLVYDIQPDDY+PY
Sbjct: 568 AIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPY 627

Query: 611 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 670
           LC L YT+ QVS+I  + + C T + I EG+LNYPSF V LG   Q F+RTVT VG    
Sbjct: 628 LCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLG-QVQTFSRTVTYVGSGRE 687

Query: 671 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 730
           VY  +++AP GVSV V PR + FS LN+K TYSVTF RI  +   +EF+EGYL WVS KH
Sbjct: 688 VYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKH 743

Query: 731 IVRSPISVK 733
           +VRSPISVK
Sbjct: 748 LVRSPISVK 743

BLAST of Csa5G157280.1 vs. TrEMBL
Match: B9RBY5_RICCO (Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 1.8e-273
Identity = 480/729 (65.84%), Postives = 577/729 (79.15%), Query Frame = 1

Query: 12  NLQTYIVHVKQPEVEILGDTIDLQNWYTSFLP-ETIEASSNEQSRLLYSYRHVISGFSAR 71
           NLQTYIVHV QPE        DL+NW+ SFL   T  +   +Q R+LYSY+++ISGFSAR
Sbjct: 43  NLQTYIVHVNQPEGRTFSQPEDLKNWHKSFLSFSTASSEEEQQQRMLYSYQNIISGFSAR 102

Query: 72  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 131
           LT+E+VK MEE  GF+SA  E  L L TTHTP +LGL+Q  GLWK+S+FGKGVIIG+LD 
Sbjct: 103 LTQEEVKAMEEITGFVSACLERKLRLQTTHTPSFLGLHQQMGLWKDSDFGKGVIIGILDG 162

Query: 132 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIG---KSPNDE 191
           G++P+HPSF+DEGMP PPAKWKGRCEF AS CNNKLIGARTFNLA     G   + P D 
Sbjct: 163 GVYPSHPSFSDEGMPLPPAKWKGRCEFNASECNNKLIGARTFNLAAKTMKGAPTEPPIDV 222

Query: 192 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVC--SPKG-CSSSDILAA 251
           +GHGTHTASTAAG FV  ++ LGNA+G AVGMAP AH+A+YKVC   P   C  SD+LA 
Sbjct: 223 DGHGTHTASTAAGGFVYNSDVLGNAKGTAVGMAPFAHLAIYKVCFGDPNDDCPESDVLAG 282

Query: 252 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 311
           LDAA+DDGVDVLSLSLG  S PFF+D IA+G+FAAI+KGIFVSCSAGNSGPSK+TL+NEA
Sbjct: 283 LDAAVDDGVDVLSLSLGDVSMPFFQDNIAIGSFAAIQKGIFVSCSAGNSGPSKSTLSNEA 342

Query: 312 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 371
           PWILTVGASTIDR+IVA+AKL +G+   GES+ QP +F +  LP+VYAG +    S +C 
Sbjct: 343 PWILTVGASTIDRRIVAIAKLGNGEELDGESVSQPSNFPTTLLPIVYAGMNSKPDSAFCG 402

Query: 372 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 431
           EG+LE +NV  K+V+CERGGGIGRIAKG  VKN GGAAMILVN + +GFST+A+AHVLP 
Sbjct: 403 EGALEGMNVKDKVVMCERGGGIGRIAKGDEVKNAGGAAMILVNDETNGFSTIADAHVLPA 462

Query: 432 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 491
           TH+S+  GLKIK YINS+  P A+I F+GT++G+ +   SPA+ SFSSRGP  ASPGILK
Sbjct: 463 THVSFAAGLKIKAYINSTKTPMATILFKGTVIGDSS---SPAVTSFSSRGPSLASPGILK 522

Query: 492 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 551
           PDI GPGV+ILAAWPFPL+N  NTNTK TFN++SGTSMSCPHLSGIAAL+KS+HP WSPA
Sbjct: 523 PDIIGPGVSILAAWPFPLDN--NTNTKLTFNIMSGTSMSCPHLSGIAALLKSSHPYWSPA 582

Query: 552 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 611
           AIKSAI+T+AD+ N +GKPIVD+  +PA+FFA G+GHVNPS+A +PGLVYDIQPDDY+PY
Sbjct: 583 AIKSAIVTTADILNMEGKPIVDETHQPADFFATGAGHVNPSRANDPGLVYDIQPDDYIPY 642

Query: 612 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 671
           LC L YTD QVSII  R ++CST+  I EG LNYPSF+V+LG   Q F RTVTNVG ANS
Sbjct: 643 LCGLNYTDEQVSIIAHRPISCSTIQTIAEGQLNYPSFSVTLG-PPQTFIRTVTNVGYANS 702

Query: 672 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 731
           V+ A + +P GV+V V P  L FSKLN+K TYS+TFS   +    SEF +GY+ WVS+K+
Sbjct: 703 VFAATITSPPGVAVSVKPSRLYFSKLNQKATYSITFSHTGYGAKTSEFGQGYITWVSDKY 762

Query: 732 IVRSPISVK 733
            V SPISV+
Sbjct: 763 FVGSPISVR 765

BLAST of Csa5G157280.1 vs. TrEMBL
Match: B9GW37_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2)

HSP 1 Score: 939.1 bits (2426), Expect = 3.2e-270
Identity = 480/728 (65.93%), Postives = 567/728 (77.88%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           T L  YIVHV +PE   + +  DL++WY SFLP +  ASS +Q R+LY+Y++V+SGF+AR
Sbjct: 33  TTLLNYIVHVAKPEGRTMAEFEDLESWYQSFLPVST-ASSEKQQRMLYAYQNVMSGFAAR 92

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E+VK+MEEKDGF+SA PE  L+L TTHTP +LGL+Q  G WK SNFGKGVIIGVLD 
Sbjct: 93  LTQEEVKSMEEKDGFLSARPERILHLQTTHTPRFLGLHQELGFWKESNFGKGVIIGVLDG 152

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKS----PND 190
           GI P+HPSF+DEGMP PPAKWKGRC+F AS CNNKLIGAR+FN+A     G +    P D
Sbjct: 153 GIFPSHPSFSDEGMPPPPAKWKGRCDFNASDCNNKLIGARSFNIAAKAKKGSAATEPPID 212

Query: 191 ENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVC---SPKGCSSSDILA 250
            +GHGTHTASTAAG FVK AE LGNARG AVG+AP AH+A+YKVC       C  SDILA
Sbjct: 213 VDGHGTHTASTAAGAFVKDAEVLGNARGTAVGIAPHAHLAIYKVCFGDPGDDCPESDILA 272

Query: 251 ALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANE 310
            LDAA+ DGVDVLSLSLG  S P F DTIA+G+FAAI+KGIFVSCSAGNSGP   TL+NE
Sbjct: 273 GLDAAVQDGVDVLSLSLGEDSVPLFNDTIAIGSFAAIQKGIFVSCSAGNSGPFNGTLSNE 332

Query: 311 APWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYC 370
           APWILTVGAST+DR+  A A+L +G+   GESL Q  +F S  LPLVYAG SG   S  C
Sbjct: 333 APWILTVGASTVDRRFSATARLGNGEQIDGESLSQHSNFPSTLLPLVYAGMSGKPNSSLC 392

Query: 371 VEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLP 430
            EG+LE ++V GKIV+CERGGGIGRIAKG  VKN GGAAMIL+N++ DGFST A+ HVLP
Sbjct: 393 GEGALEGMDVKGKIVLCERGGGIGRIAKGGEVKNAGGAAMILMNEEADGFSTNADVHVLP 452

Query: 431 TTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGIL 490
            TH+S+  GLKIK YINS+  P A+I F+GT++G+ +   SP +ASFSSRGP  ASPGIL
Sbjct: 453 ATHVSFAAGLKIKAYINSTQAPMATILFKGTVIGDSS---SPFVASFSSRGPSLASPGIL 512

Query: 491 KPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSP 550
           KPDI GPGV+ILAAWPFPL+N  NTN+KSTFN+ISGTSMSCPHLSGIAAL+KS+HP WSP
Sbjct: 513 KPDIIGPGVSILAAWPFPLDN--NTNSKSTFNIISGTSMSCPHLSGIAALLKSSHPYWSP 572

Query: 551 AAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVP 610
           AAIKSAIMT+AD  N +GK IVDQ L+PA+ FA G+GHVNPS+A NPGLVYDIQPDDY+P
Sbjct: 573 AAIKSAIMTTADTLNMEGKLIVDQTLQPADIFATGAGHVNPSRANNPGLVYDIQPDDYIP 632

Query: 611 YLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDAN 670
           YLC L Y D +VSIIV  QV CS    I EG+LNYPSFAV+LG  SQ F RTVTNVGD N
Sbjct: 633 YLCGLGYADNEVSIIVHEQVKCSEKPSIPEGELNYPSFAVTLG-PSQTFTRTVTNVGDVN 692

Query: 671 SVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNK 730
           S Y   + +P GV V V P  L FSK+N+K TYSV FSR ++    SE ++GY++W S K
Sbjct: 693 SAYEVAIVSPPGVDVTVKPSKLYFSKVNQKATYSVAFSRTEYGGKTSETAQGYIVWASAK 752

BLAST of Csa5G157280.1 vs. TrEMBL
Match: A0A067JNI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 6.3e-266
Identity = 466/730 (63.84%), Postives = 566/730 (77.53%), Query Frame = 1

Query: 10  NTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSA 69
           N NLQ YIVHV  PE        +L+NW+ SFLP +  +S  +Q R+LYSY ++ISGFSA
Sbjct: 32  NKNLQAYIVHVSPPEGRTFSQRENLENWHKSFLPFSTASSEKQQKRMLYSYHNIISGFSA 91

Query: 70  RLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLD 129
           RLT E+VK MEE +GF+ A PE  L+L TTHTP +LGL++  G WK SNFGKGVIIGVLD
Sbjct: 92  RLTHEEVKAMEEINGFVLARPERKLHLQTTHTPSFLGLHRQMGFWKESNFGKGVIIGVLD 151

Query: 130 TGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIG---KSPND 189
            G+ P+HPSFND+GMP PPAKWKGRCEF AS CNNKLIGAR+FNLA     G   ++P D
Sbjct: 152 GGVFPSHPSFNDKGMPPPPAKWKGRCEFNASKCNNKLIGARSFNLAAKAMKGIAAETPID 211

Query: 190 ENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVC--SPKG-CSSSDILA 249
            +GHGTHTASTAAG+FV  A  LGNA+G AVGMAP AH+A+YKVC   P   C  SDILA
Sbjct: 212 VDGHGTHTASTAAGSFVYNANVLGNAKGTAVGMAPYAHLAIYKVCFGDPNDDCPESDILA 271

Query: 250 ALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANE 309
            LDAAI DGVDVLSLS+G  S PFF+D IA+G+FAAI+KGIFVSC+AGNSGP   TL+NE
Sbjct: 272 GLDAAIQDGVDVLSLSIGDISMPFFQDNIAIGSFAAIQKGIFVSCAAGNSGPFNGTLSNE 331

Query: 310 APWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYC 369
           APWILTVGASTIDRKI A AKL +G+   GES+ QP +F +  LPLVY G +G   S +C
Sbjct: 332 APWILTVGASTIDRKIAATAKLGNGEELDGESVLQPSNFPTTLLPLVYPGMNGKTESAFC 391

Query: 370 VEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLP 429
            E +++ ++V  K+V+CERGGGIGR+AKG  VKN GGAAMIL+N +  GFST+A+AHVLP
Sbjct: 392 SERAVQGMDVKDKVVLCERGGGIGRVAKGEEVKNAGGAAMILINDEISGFSTIADAHVLP 451

Query: 430 TTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGIL 489
            TH+S+  GL+IK YINS+  P A+I F+GT++G+     SPA+ SFSSRGP  ASPGIL
Sbjct: 452 ATHVSFAAGLQIKAYINSTKTPMATILFKGTVIGD---PLSPAVTSFSSRGPNLASPGIL 511

Query: 490 KPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSP 549
           KPDI GPGV+ILAAWPFPL+N   TNTKSTFN++SGTSM+CPHLSGIAAL+KS+HP WSP
Sbjct: 512 KPDIIGPGVSILAAWPFPLDN--TTNTKSTFNLVSGTSMACPHLSGIAALLKSSHPYWSP 571

Query: 550 AAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVP 609
           AAIKSAIMT+AD+ N +G PIVD+  +PA+ F +G+GHVNPS+A +PGL+YDIQPDDY+P
Sbjct: 572 AAIKSAIMTTADIFNMEGSPIVDEKHQPADLFTIGAGHVNPSRANDPGLIYDIQPDDYIP 631

Query: 610 YLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDAN 669
           YLC L Y + QVSII  R++ CS    I EG LNYPSF+V+LGA SQ F RTVTNVG+AN
Sbjct: 632 YLCGLGYKEEQVSIIAHRRIKCSEKLSIPEGQLNYPSFSVTLGA-SQTFTRTVTNVGEAN 691

Query: 670 SVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNK 729
           SVY A +  P GV+V V P  L FS++N+K+TYSVTFS        SEF++GY++W S K
Sbjct: 692 SVYAATIVPPPGVAVTVEPYRLYFSQVNQKVTYSVTFSPTGSSGKTSEFAQGYILWSSAK 751

Query: 730 HIVRSPISVK 733
           H+VRSPISV+
Sbjct: 752 HLVRSPISVR 755

BLAST of Csa5G157280.1 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 622.5 bits (1604), Expect = 3.4e-178
Identity = 347/748 (46.39%), Postives = 469/748 (62.70%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQ-NWYTSFLPETIEASSNEQSRLLYSYRHVIS 66
           S+S+++  TYIVH+ + ++     + DL  NWY S L      S ++ + LLY+Y + I 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMP---SSFDLHSNWYDSSL-----RSISDSAELLYTYENAIH 82

Query: 67  GFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFG-LWKNSNFGKGVI 126
           GFS RLT+E+  ++  + G IS +PE    LHTT TP +LGL++H   L+  +     V+
Sbjct: 83  GFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVV 142

Query: 127 IGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTFNLANNVSI 186
           +GVLDTG+ P   S++DEG    P+ WKG CE G    AS+CN KLIGAR F      ++
Sbjct: 143 VGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTM 202

Query: 187 G--------KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
           G        +SP D++GHGTHT+STAAG+ V+GA  LG A G A GMAP A +AVYKVC 
Sbjct: 203 GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW 262

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 306
             GC SSDILAA+D AI D V+VLS+SLG   + +++D +A+GAFAA+++GI VSCSAGN
Sbjct: 263 LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGN 322

Query: 307 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYA 366
           +GPS ++L+N APWI TVGA T+DR   ALA L +GK FTG SLF+      K LP +YA
Sbjct: 323 AGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA 382

Query: 367 GK-SGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPD 426
           G  S       C+ G+L    V GKIV+C+RG    R+ KG VVK  GG  MIL N   +
Sbjct: 383 GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAAN 442

Query: 427 GFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFS 486
           G   +A+AH+LP T +  + G  I+ Y+ +  NP ASIS  GT++G +    SP +A+FS
Sbjct: 443 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP---SPVVAAFS 502

Query: 487 SRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSG 546
           SRGP   +P ILKPD+  PGVNILAAW          + + +  FN+ISGTSMSCPH+SG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 547 IAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAA 606
           +AAL+KS HP WSPAAI+SA+MT+A      GKP++D    KP+  F  G+GHV+P+ A 
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 622

Query: 607 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSL-GA 666
           NPGL+YD+  +DY+ +LC L YT  Q+  + RR  TC         DLNYPSFAV++ G 
Sbjct: 623 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 682

Query: 667 DSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVR 726
            +  + RTVT+VG A +    +     GV + V P  L F + NEK +Y+VTF+ +D  +
Sbjct: 683 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSK 742

Query: 727 TRSEFSEGYLIWVSNKHIVRSPISVKLT 735
                S G + W   KH+V SP+++  T
Sbjct: 743 PSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Csa5G157280.1 vs. TAIR10
Match: AT1G04110.1 (AT1G04110.1 Subtilase family protein)

HSP 1 Score: 619.8 bits (1597), Expect = 2.2e-177
Identity = 351/754 (46.55%), Postives = 473/754 (62.73%), Query Frame = 1

Query: 14  QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETI----EASSNEQSRLLYSYRHVISGFSA 73
           QTYIV +  P  E         +W+ SFL E +    E      SRLLYSY   I GF+A
Sbjct: 26  QTYIVQL-HPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAA 85

Query: 74  RLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQ--HFGLWKNSNFGKGVIIGV 133
           +LT+ + + +      ++  P+  L + TT++ ++LGL+   + G+W  S FG+G IIGV
Sbjct: 86  QLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGV 145

Query: 134 LDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV--SIG 193
           LDTG+ P  PSF+D GMPS P KWKG C+    F +S CN KLIGAR F   + V  S  
Sbjct: 146 LDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPE 205

Query: 194 KSPN---------DENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 253
           +SPN         D  GHGTHTAST  G+ V  A  LGN  G A GMAP AHIAVYKVC 
Sbjct: 206 ESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCW 265

Query: 254 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 313
             GC SSDILAA+D AI D VDVLSLSLG    P + DTIA+G F A+++GI V C+AGN
Sbjct: 266 FNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGN 325

Query: 314 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDF--SSKFLPLV 373
           +GP ++++AN APW+ T+GA T+DR+  A+ +L +GK+  GESL+  +    + + + ++
Sbjct: 326 NGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVI 385

Query: 374 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 433
           Y    G +GSE+C+ GSL +  + GK+V+C+RG   GR  KG  VK  GG AMIL N + 
Sbjct: 386 YV-TGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 434 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 493
           +      + H+LP T + Y + + +K Y+N++  PKA I F GT++G    + +P +A F
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGR---SRAPEVAQF 505

Query: 494 SSRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLS 553
           S+RGP  A+P ILKPD+  PGVNI+AAWP  L        + +  F V+SGTSMSCPH+S
Sbjct: 506 SARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVS 565

Query: 554 GIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAA 613
           GI ALI+S +PNWSPAAIKSA+MT+AD+ + QGK I D + KPA  FA+G+GHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 614 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREG-DLNYPSFAV--SL 673
           NPGLVY+IQP DY+ YLC L +T + +  I  + V+C+ + R   G  LNYPS AV    
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685

Query: 674 GADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTF--SRI 733
           G  ++   R VTNVG  NS+Y   VKAP G+ V V P+ L F  +++ L+Y V F   + 
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745

BLAST of Csa5G157280.1 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 601.7 bits (1550), Expect = 6.2e-172
Identity = 350/759 (46.11%), Postives = 456/759 (60.08%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISG 66
           S+S+  L++YIVHV++     L  + +  NW+ S L      SS + + LLYSY   + G
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLLRSL--PSSPQPATLLYSYSRAVHG 83

Query: 67  FSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 126
           FSARL+  Q   +      IS +P+    +HTTHTP +LG +Q+ GLW NSN+G+ VI+G
Sbjct: 84  FSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVG 143

Query: 127 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTF---------- 186
           VLDTGI P HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F          
Sbjct: 144 VLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNG 203

Query: 187 NLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
              +     +SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C 
Sbjct: 204 TKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICW 263

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPST--PFFKDTIAVGAFAAIKKGIFVSCSA 306
             GC  SDILAA+D A+ DGV V+SLS+GA  +   +  D+IA+GAF A + GI VSCSA
Sbjct: 264 TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSA 323

Query: 307 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 366
           GNSGP+  T  N APWILTVGAST+DR+  A A    GKVFTG SL+         L LV
Sbjct: 324 GNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLV 383

Query: 367 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 426
           Y+G     GS  C  G L    V GKIV+C+RGG   R+ KG  VK  GGA MIL N   
Sbjct: 384 YSGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAE 443

Query: 427 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 486
            G    A++H++P T +  + G +I++YI +S +P A ISF GTL+G   +  SP +A+F
Sbjct: 444 SGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG--PSPPSPRVAAF 503

Query: 487 SSRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTNTKSTFNVISGTSMSCP 546
           SSRGP   +P ILKPD+  PGVNILA W     P  L+ +     +  FN+ISGTSMSCP
Sbjct: 504 SSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR---RVQFNIISGTSMSCP 563

Query: 547 HLSGIAALIKSNHPNWSPAAIKSAIMTSA-DVRNPQGKPIVDQDL-KPANFFAMGSGHVN 606
           H+SG+AAL++  HP+WSPAAIKSA++T+A DV N  G+PI D    K +N F  G+GHV+
Sbjct: 564 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHVD 623

Query: 607 PSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVT----CSTVSRIREGDLNYPS 666
           P+KA NPGLVYDI+  +YV +LC +  +    ++  +  T    C T      GDLNYPS
Sbjct: 624 PNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPS 683

Query: 667 FAVSLGADSQA--FNRTVTNVG-DANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYS 726
           F+V   +  +   + R V NVG + ++VY   VK+PA V + V+P  L FSK    L Y 
Sbjct: 684 FSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYE 743

Query: 727 VTFSRIDF---VRTRSEFSEGYLIWVSNKHIVRSPISVK 733
           VTF  +     V +      G + W   +H+V+SP++V+
Sbjct: 744 VTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of Csa5G157280.1 vs. TAIR10
Match: AT1G01900.1 (AT1G01900.1 subtilase family protein)

HSP 1 Score: 589.0 bits (1517), Expect = 4.2e-168
Identity = 339/754 (44.96%), Postives = 457/754 (60.61%), Query Frame = 1

Query: 5   LWSASNTNL--QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRH 64
           ++ ASN +   QTY++H      + +  ++     + S   E I         + Y Y +
Sbjct: 31  IFFASNVSSRKQTYVIHTVTTSTKHIVTSL-----FNSLQTENINDDDFSLPEIHYIYEN 90

Query: 65  VISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKG 124
            +SGFSA LT +Q+ T++   GFISA P+  L+LHTT++ E+LGL    GLW  ++    
Sbjct: 91  AMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSD 150

Query: 125 VIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV 184
           VIIG++DTGI P H SF D  M   P++W+G C+    F +S CN K+IGA  F      
Sbjct: 151 VIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYES 210

Query: 185 SIGK--------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKV 244
            +GK        S  D  GHGTHTASTAAG  V  A   G A+G A GM   + IA YK 
Sbjct: 211 IVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKA 270

Query: 245 CSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSA 304
           C   GC+S+D++AA+D AI DGVDV+SLSLG  S PF+ D IA+  F A++K IFVSCSA
Sbjct: 271 CWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSA 330

Query: 305 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 364
           GNSGP+ +T++N APW++TV AS  DR   A+ ++ + K   G SL++ +  S K LPL 
Sbjct: 331 GNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGK--SLKNLPLA 390

Query: 365 YAGKSGIE-GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQK 424
           +   +G E G+ +C+  SL++  V GKIV+C RG   GR AKG  VK  GGAAM+LV+ +
Sbjct: 391 FNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAKGEEVKRSGGAAMLLVSTE 450

Query: 425 PDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMAS 484
            +G   LA+ HVLP   L + DG  +  Y+  + N  AS+ F GT  G  A    P +A+
Sbjct: 451 AEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYGATA----PMVAA 510

Query: 485 FSSRGPCQASPGILKPDITGPGVNILAAW-PFPLNNNTNTNTKST-FNVISGTSMSCPHL 544
           FSSRGP  A P I KPDI  PG+NILA W PF   +   ++ +   FN+ISGTSM+CPH+
Sbjct: 511 FSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHI 570

Query: 545 SGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDL----KPANFFAMGSGHVN 604
           SGIAALIKS H +WSPA IKSAIMT+A + + + +PI D+        A  FA G+G+V+
Sbjct: 571 SGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVD 630

Query: 605 PSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTC-STVSRIREGDLNYPSFA 664
           P++A +PGLVYD    DY+ YLC L YT  ++ +      TC S    +  GDLNYPSFA
Sbjct: 631 PTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFA 690

Query: 665 VSL--GADSQA--FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSV 724
           V+L  GA+ +   + RTVTNVG     Y   V+ P GV VRV P+ LKF K  E+L+Y+V
Sbjct: 691 VNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTV 750

Query: 725 TFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISV 732
           T+   +  R  S  S G L+W+ +K+ VRSPI+V
Sbjct: 751 TYD-AEASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of Csa5G157280.1 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 584.3 bits (1505), Expect = 1.0e-166
Identity = 335/743 (45.09%), Postives = 446/743 (60.03%), Query Frame = 1

Query: 11  TNLQTYIV---HVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGF 70
           T  +TYI+   H  +PE       +   +WYTS L        N +S LLY+Y     GF
Sbjct: 25  TAKKTYIIRVNHSDKPE-----SFLTHHDWYTSQL--------NSESSLLYTYTTSFHGF 84

Query: 71  SARLTKEQVKTM-EEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 130
           SA L   +  ++    +  +    +    LHTT TPE+LGLN  FG+    +   GVIIG
Sbjct: 85  SAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIG 144

Query: 131 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNVSIGK 190
           VLDTG+ P   SF+D  MP  P+KWKG CE    F + +CN KLIGAR+F+    ++ G 
Sbjct: 145 VLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGG 204

Query: 191 ---------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSP 250
                    SP D +GHGTHT++TAAG+ V+ A  LG A G A GMA  A +A YKVC  
Sbjct: 205 GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWS 264

Query: 251 KGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNS 310
            GC  SDILAA+D AI DGVDVLSLSLG  S P+++DTIA+GAF+A+++G+FVSCSAGNS
Sbjct: 265 TGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNS 324

Query: 311 GPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAG 370
           GP++ ++AN APW++TVGA T+DR   A A L +GK  TG SL+      +K L LVY  
Sbjct: 325 GPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-N 384

Query: 371 KSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGF 430
           K     S  C+ GSL+   V GKIVVC+RG    R+ KG VV++ GG  MI+ N    G 
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGE 444

Query: 431 STLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSR 490
             +A++H+LP   +  + G  ++EY+ S   P A + F+GT+L  +    SP +A+FSSR
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP---SPVVAAFSSR 504

Query: 491 GPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSGIA 550
           GP   +P ILKPD+ GPGVNILA W   +        + ++ FN++SGTSMSCPH+SG+A
Sbjct: 505 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 564

Query: 551 ALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAANP 610
            L+K+ HP WSP+AIKSA+MT+A V +    P+ D  D   +N +A GSGHV+P KA +P
Sbjct: 565 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSP 624

Query: 611 GLVYDIQPDDYVPYLCHL-YT-DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADS 670
           GLVYDI  ++Y+ +LC L YT D  V+I+ R  V CS       G LNYPSF+V  G   
Sbjct: 625 GLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK-KFSDPGQLNYPSFSVLFGGKR 684

Query: 671 QA-FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRT 730
              + R VTNVG A+SVY   V     V + V P  L F  + EK  Y+VTF     V  
Sbjct: 685 VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM 744

BLAST of Csa5G157280.1 vs. NCBI nr
Match: gi|449459730|ref|XP_004147599.1| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 1

Query: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60
           MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY
Sbjct: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60

Query: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120
           RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG
Sbjct: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120

Query: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180
           KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180

Query: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240

Query: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300
           ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360
           ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360

Query: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420
           EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH
Sbjct: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420

Query: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480
           VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540

Query: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600
           WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660
           YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD
Sbjct: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660

Query: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720
           ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS
Sbjct: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720

Query: 721 NKHIVRSPISVKLT 735
           NKHIVRSPISVKLT
Sbjct: 721 NKHIVRSPISVKLT 734

BLAST of Csa5G157280.1 vs. NCBI nr
Match: gi|659073650|ref|XP_008437177.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 688/734 (93.73%), Postives = 712/734 (97.00%), Query Frame = 1

Query: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60
           MKTEL S SNTNLQTYIVHVKQP +EILGDTIDLQNWYTSFLPETIEASS+EQ RLLYSY
Sbjct: 21  MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 80

Query: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120
           RHV+SGFSARLTKEQVK MEEKDGFISAMPETTLNLHTTHTPEYLGLN+HFGLWKNSNFG
Sbjct: 81  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNFG 140

Query: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180
           KGVIIGVLDTGIHPNHPSFNDEGM SPPAKWKGRCEFGASICNNKLIGARTFNLANN  I
Sbjct: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 200

Query: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIA+YKVCSP GCSSSD
Sbjct: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 260

Query: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300
           ILAALDAAIDDGVDVLSLSLGAPSTPFF+DTIA+GAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 261 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNTL 320

Query: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360
           ANEAPWILTVGASTIDRKIVALAKL SG+V+TGESLFQPR+F SKFLPLVYAGKSGIEGS
Sbjct: 321 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 380

Query: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420
           EYC++GSLEKLNVTGK+VVCERGGGI R+AKGLVVKNGGGAAMIL+NQKP+GFSTLAEAH
Sbjct: 381 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 440

Query: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480
           VLPTTHLSYEDGLKIK YINSSHNPKASISF+GTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 500

Query: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKS HPN
Sbjct: 501 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 560

Query: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600
           WSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 561 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620

Query: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660
           Y+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSFAVSLGA SQ FNRTVTNVGD
Sbjct: 621 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGD 680

Query: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720
           ANSVYY IV+APAGVSV+VTP NLKFSKLNEK+TYSVTFSRIDFVRT SEFSEGYLIWVS
Sbjct: 681 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 740

Query: 721 NKHIVRSPISVKLT 735
            K +VRSPISVKLT
Sbjct: 741 KKLMVRSPISVKLT 754

BLAST of Csa5G157280.1 vs. NCBI nr
Match: gi|659074655|ref|XP_008437722.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1061.6 bits (2744), Expect = 6.2e-307
Identity = 521/726 (71.76%), Postives = 618/726 (85.12%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTID--LQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFS 70
           + LQTYIVHV+QP++ ++G++ +  +++WY SF+P++ E +  EQ +LLYSYR+V+SGFS
Sbjct: 41  SKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTV-EQPQLLYSYRNVMSGFS 100

Query: 71  ARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVL 130
           ARLT EQVK ME+KDGFISAMPET ++LHTTHTPEYLGLNQ FG+WKNSNFGKGVI+GVL
Sbjct: 101 ARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVL 160

Query: 131 DTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDEN 190
           DTGIHPNHPSFNDEGM  PPAKWKG+CEF +S+CNNKLIGARTFNL NN  + +SPNDE 
Sbjct: 161 DTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEK 220

Query: 191 GHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAA 250
           GHGTHTASTAAG FV+ AEALGNA+GKA G+APLAH+A+YKVCS K C SSD+ A +DAA
Sbjct: 221 GHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAA 280

Query: 251 IDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWIL 310
           IDDGVDVLS+SLG+ S PFFKD IAV  F AI+KGIFVS SAGNSGP  +TL+N+APWIL
Sbjct: 281 IDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWIL 340

Query: 311 TVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSL 370
           TVGASTI+R+IVA+AKL +G+ + GESL+QP DF SKFLPLVYAG    +   +C EGSL
Sbjct: 341 TVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSL 400

Query: 371 EKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLS 430
           E ++V GK+VVCE  GG+GR+AKGLVVKN GGAAMIL+NQ+ DGFSTL+EAHVLP TH+S
Sbjct: 401 ENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVS 460

Query: 431 YEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDIT 490
           Y+ G+ IK YINSS NP ASISF+GT++G+     +P+MASFSSRGPC  SPGILKPDIT
Sbjct: 461 YKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDIT 520

Query: 491 GPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKS 550
           GPGVNILAAWPFPL+N  +TNTKSTFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKS
Sbjct: 521 GPGVNILAAWPFPLDN--DTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKS 580

Query: 551 AIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL 610
           AIMT+A+++ PQG+PI DQDL+PANFFAMG+GHVNPSKAA+PGLVYDIQPDDY+PYLC L
Sbjct: 581 AIMTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGL 640

Query: 611 YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAI 670
           Y D +VSIIV R V C  V  IREGDLNYPSF+V+LG   Q F RTVTNVG+ANSVY AI
Sbjct: 641 YKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGG-LQTFKRTVTNVGEANSVYTAI 700

Query: 671 VKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWV--SNKHIVR 730
           V+AP GVS+ V PR L FS++N+ +T++VTF+RI +V+   EF EGYL WV  S K++VR
Sbjct: 701 VEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVR 760

Query: 731 SPISVK 733
           SP+SVK
Sbjct: 761 SPVSVK 762

BLAST of Csa5G157280.1 vs. NCBI nr
Match: gi|449459724|ref|XP_004147596.1| (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus])

HSP 1 Score: 963.8 bits (2490), Expect = 1.8e-277
Identity = 490/729 (67.22%), Postives = 578/729 (79.29%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           +NL TYIVHVK+PEV       DL++W+ SFLP ++E +S EQ  LLYSYR+V+SGFSAR
Sbjct: 28  SNLHTYIVHVKKPEV-----VDDLESWHRSFLPTSLE-NSEEQPTLLYSYRNVMSGFSAR 87

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E VK MEEKDGF+SA  ET ++LHTTH+P +LGLN+ FG WK+SNFGKGVIIGVLD 
Sbjct: 88  LTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGVLDG 147

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGK------SP 190
           GI P+HPSF D GMP PPAKWKGRCEF  S CNNKLIGAR+ NLA+    GK      SP
Sbjct: 148 GITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGARSLNLASQALKGKITTLDDSP 207

Query: 191 NDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAA 250
            DE+GHGTHTASTAAGTFV GAEALGNA G AVGMAPLAH+A+YKVC  + CS+ DILA 
Sbjct: 208 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDILAG 267

Query: 251 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 310
           LDAA++DGVDVLS+SLG P  PFF D  A+GAFAAI+KGIFVSCSA NSGP   TL+NEA
Sbjct: 268 LDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLSNEA 327

Query: 311 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 370
           PWILTV ASTIDRKI A AKL +G+ F GESLFQP DF   FLPLV+ G+   E    C 
Sbjct: 328 PWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKN-ETVALCA 387

Query: 371 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 430
           EGSL+ ++V GK+VVC+RGGGI RIAKG+ VKN GGAAMIL+N + DGF+T A+AHVLP 
Sbjct: 388 EGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHVLPA 447

Query: 431 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 490
           +H+S+   LKIK YINS+  P A+I F+GT +G+    FSPA+A+FSSRGP  ASPGILK
Sbjct: 448 SHVSHTAALKIKAYINSTTYPTATIVFKGTTIGD---DFSPAIAAFSSRGPSLASPGILK 507

Query: 491 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 550
           PDITGPGV+ILAAWPFPL+N  NTNTKSTFN++SGTSMSCPHLSGIAALIKS HP+WSPA
Sbjct: 508 PDITGPGVSILAAWPFPLDN--NTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPA 567

Query: 551 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 610
           AIKS+IMT+A++ N +G PIVDQ L+PA+ FA+G+GHVNPSKA +PGLVYDIQPDDY+PY
Sbjct: 568 AIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPY 627

Query: 611 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 670
           LC L YT+ QVS+I  + + C T + I EG+LNYPSF V LG   Q F+RTVT VG    
Sbjct: 628 LCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLG-QVQTFSRTVTYVGSGRE 687

Query: 671 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 730
           VY  +++AP GVSV V PR + FS LN+K TYSVTF RI  +   +EF+EGYL WVS KH
Sbjct: 688 VYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKH 743

Query: 731 IVRSPISVK 733
           +VRSPISVK
Sbjct: 748 LVRSPISVK 743

BLAST of Csa5G157280.1 vs. NCBI nr
Match: gi|659073656|ref|XP_008437181.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 961.1 bits (2483), Expect = 1.1e-276
Identity = 490/729 (67.22%), Postives = 575/729 (78.88%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           +NL TYIVHVK+PEV       DL+ W+ SFLP +++ +  EQ  LLYSYR+V+SGFSAR
Sbjct: 28  SNLHTYIVHVKKPEV-----VDDLEIWHRSFLPTSLD-NEEEQPTLLYSYRNVMSGFSAR 87

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E VK MEEKDGF+SA  ET ++LHTTHTP++LGLN+ FG WK+SNFGKGVIIGVLD 
Sbjct: 88  LTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDG 147

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGK------SP 190
           GI PNHPSF+D GM  PPAKWKGRCEF  S CNNKLIGAR+ NLA+    GK      SP
Sbjct: 148 GITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGARSMNLASQALKGKITTLDDSP 207

Query: 191 NDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAA 250
            DE+GHGTHTASTAAGTFV GAEALGNA G AVGMAPLAH+A+YKVC  + CS  DILA 
Sbjct: 208 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGEDCSDVDILAG 267

Query: 251 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 310
           LDAA++DGVDVLS+SLG PS PFF D  A+G+FAAI+KGIFVSCSA NSGP   TL+NEA
Sbjct: 268 LDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNATLSNEA 327

Query: 311 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 370
           PWILTV ASTIDRKI A AKL +G+ F GESLFQP DF    LPLV+ G+   E    C 
Sbjct: 328 PWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTLLPLVFPGEKN-ETVALCA 387

Query: 371 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 430
           EGSL+ ++V GK+VVCERGGGI RIAKG+ VKNGGGAAMIL+N + DGF+T  +AHVLP 
Sbjct: 388 EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTTEVDAHVLPA 447

Query: 431 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 490
           +H+S+   LKIK YINS+  P A+I F+GT +G+    FSPA+ASFSSRGP  ASPGILK
Sbjct: 448 SHVSHTAALKIKAYINSTTYPTATILFKGTTIGD---DFSPAIASFSSRGPSLASPGILK 507

Query: 491 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 550
           PDITGPGV+ILAAWPFPL+N  NTNTKSTFN+ISGTSMSCPHLSGIAALIKS HP+WSPA
Sbjct: 508 PDITGPGVSILAAWPFPLDN--NTNTKSTFNIISGTSMSCPHLSGIAALIKSAHPDWSPA 567

Query: 551 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 610
           AIKS+IMT+A++ N +G PI+D+ L+PA+ FA+G+GHVNPSKA +PGLVYDIQPDDY+PY
Sbjct: 568 AIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPY 627

Query: 611 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 670
           LC L YT+ QVS+I  + + C T S I EG+LNYPSF V LG   Q F+RTVT+VG    
Sbjct: 628 LCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLG-PVQTFSRTVTSVGSGRV 687

Query: 671 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 730
           VY  +++AP GVSV V PR L FS LN+K TYSVTF R   +    EF+EGYL WVS KH
Sbjct: 688 VYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSIEFAEGYLKWVSAKH 743

Query: 731 IVRSPISVK 733
           +VRSPISVK
Sbjct: 748 VVRSPISVK 743

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT17_ARATH6.0e-17746.39Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT12_ARATH3.9e-17646.55Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1[more]
SBT14_ARATH1.1e-17046.11Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1[more]
SBT11_ARATH7.4e-16744.96Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1[more]
SBT18_ARATH1.8e-16545.09Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMQ7_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1[more]
A0A0A0KKE3_CUCSA1.2e-27767.22Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1[more]
B9RBY5_RICCO1.8e-27365.84Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1[more]
B9GW37_POPTR3.2e-27065.93Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2[more]
A0A067JNI9_JATCU6.3e-26663.84Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G67360.13.4e-17846.39 Subtilase family protein[more]
AT1G04110.12.2e-17746.55 Subtilase family protein[more]
AT3G14067.16.2e-17246.11 Subtilase family protein[more]
AT1G01900.14.2e-16844.96 subtilase family protein[more]
AT2G05920.11.0e-16645.09 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|449459730|ref|XP_004147599.1|0.0e+00100.00PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus][more]
gi|659073650|ref|XP_008437177.1|0.0e+0093.73PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|659074655|ref|XP_008437722.1|6.2e-30771.76PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|449459724|ref|XP_004147596.1|1.8e-27767.22PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus][more]
gi|659073656|ref|XP_008437181.1|1.1e-27667.22PREDICTED: subtilisin-like protease [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023827Peptidase_S8_Asp-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csa5G157280Csa5G157280gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csa5G157280.1Csa5G157280.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csa5G157280.1.cds1Csa5G157280.1.cds1CDS


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 464..592
score: 2.2E-84coord: 71..315
score: 2.2
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 120..556
score: 8.3
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 96..354
score: 3.8E-84coord: 467..592
score: 3.8
IPR003137PA domainPFAMPF02225PAcoord: 348..435
score: 3.2
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 15..97
score: 5.3
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 186..199
score: 1.7E-20coord: 120..139
score: 1.7E-20coord: 518..534
score: 1.7
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 10..733
score:
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 125..136
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 342..444
score: 7.1
NoneNo IPR availablePANTHERPTHR10795:SF370SUBFAMILY NOT NAMEDcoord: 10..733
score:
NoneNo IPR availableunknownSSF52025PA domaincoord: 348..442
score: 3.6