Csa5G157280 (gene) Cucumber (Chinese Long) v2

NameCsa5G157280
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionSubtilisin-like serine protease; contains IPR015500 (Peptidase S8, subtilisin-related), IPR023827 (Peptidase S8, subtilisin, Asp-active site)
LocationChr5 : 5539360 .. 5541564 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACTGAGCTTTGGAGTGCTTCCAACACCAATTTACAAACCTACATTGTTCATGTTAAGCAACCAGAGGTTGAGATTTTGGGTGACACCATTGATCTACAAAATTGGTACACATCATTCTTACCAGAAACGATCGAAGCGTCATCCAACGAACAATCACGGTTACTTTATTCATATCGACACGTGATCAGTGGTTTCTCTGCAAGACTTACAAAAGAACAAGTTAAAACAATGGAAGAGAAGGATGGTTTCATCTCAGCCATGCCTGAAACCACATTGAATTTGCACACAACTCACACACCTGAATATTTGGGGTTGAACCAACATTTTGGGTTATGGAAAAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGCCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAACAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATGTTTCGATTGGGAAATCACCAAATGACGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTTGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCAGTTGGAATGGCACCTTTAGCTCATATTGCAGTGTACAAAGTTTGTTCTCCAAAAGGTTGTTCCAGTAGTGATATACTTGCTGCCTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCTTCGACTCCATTTTTCAAAGACACAATTGCCGTAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCAGGGAATTCAGGCCCTTCCAAAAATACATTAGCCAACGAAGCACCATGGATTTTAACTGTTGGAGCTAGCACCATTGATAGAAAAATCGTGGCATTGGCAAAGCTTGAAAGTGGCAAAGTCTTTACCGGTGAATCTCTGTTCCAGCCAAGAGATTTCTCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGTTCTGAATATTGTGTTGAAGGTTCACTTGAAAAGCTGAATGTGACAGGAAAAATCGTGGTATGTGAACGAGGAGGAGGAATAGGCAGAATAGCAAAAGGGTTGGTTGTGAAAAATGGTGGCGGCGCTGCCATGATTCTTGTAAACCAAAAACCAGATGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACAACCCATTTAAGCTATGAAGATGGACTTAAAATCAAAGAATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTGAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCTATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACAGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAACACAAACACGAACACAAAATCGACTTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTAATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCGTGGATCAAGATTTGAAACCAGCGAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTTTATGACATTCAGCCCGATGATTATGTTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAGACAAGTAACGTGTTCGACGGTATCAAGAATACGAGAAGGCGATCTGAATTATCCTTCGTTTGCTGTGAGTTTGGGAGCTGATTCACAGGCGTTTAATAGAACAGTGACAAATGTGGGTGATGCTAATTCAGTTTATTACGCCATTGTTAAGGCACCAGCTGGAGTTTCAGTGAGAGTTACACCAAGAAATTTGAAGTTCTCAAAGTTGAATGAAAAATTGACATATTCTGTAACTTTCAGCCGGATCGACTTTGTTAGAACAAGAAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAATAAACACATAGTGAGAAGTCCAATCTCTGTAAAGCTCACCTAA

mRNA sequence

ATGAAAACTGAGCTTTGGAGTGCTTCCAACACCAATTTACAAACCTACATTGTTCATGTTAAGCAACCAGAGGTTGAGATTTTGGGTGACACCATTGATCTACAAAATTGGTACACATCATTCTTACCAGAAACGATCGAAGCGTCATCCAACGAACAATCACGGTTACTTTATTCATATCGACACGTGATCAGTGGTTTCTCTGCAAGACTTACAAAAGAACAAGTTAAAACAATGGAAGAGAAGGATGGTTTCATCTCAGCCATGCCTGAAACCACATTGAATTTGCACACAACTCACACACCTGAATATTTGGGGTTGAACCAACATTTTGGGTTATGGAAAAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGCCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAACAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATGTTTCGATTGGGAAATCACCAAATGACGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTTGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCAGTTGGAATGGCACCTTTAGCTCATATTGCAGTGTACAAAGTTTGTTCTCCAAAAGGTTGTTCCAGTAGTGATATACTTGCTGCCTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCTTCGACTCCATTTTTCAAAGACACAATTGCCGTAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCAGGGAATTCAGGCCCTTCCAAAAATACATTAGCCAACGAAGCACCATGGATTTTAACTGTTGGAGCTAGCACCATTGATAGAAAAATCGTGGCATTGGCAAAGCTTGAAAGTGGCAAAGTCTTTACCGGTGAATCTCTGTTCCAGCCAAGAGATTTCTCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGTTCTGAATATTGTGTTGAAGGTTCACTTGAAAAGCTGAATGTGACAGGAAAAATCGTGGTATGTGAACGAGGAGGAGGAATAGGCAGAATAGCAAAAGGGTTGGTTGTGAAAAATGGTGGCGGCGCTGCCATGATTCTTGTAAACCAAAAACCAGATGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACAACCCATTTAAGCTATGAAGATGGACTTAAAATCAAAGAATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTGAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCTATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACAGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAACACAAACACGAACACAAAATCGACTTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTAATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCGTGGATCAAGATTTGAAACCAGCGAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTTTATGACATTCAGCCCGATGATTATGTTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAGACAAGTAACGTGTTCGACGGTATCAAGAATACGAGAAGGCGATCTGAATTATCCTTCGTTTGCTGTGAGTTTGGGAGCTGATTCACAGGCGTTTAATAGAACAGTGACAAATGTGGGTGATGCTAATTCAGTTTATTACGCCATTGTTAAGGCACCAGCTGGAGTTTCAGTGAGAGTTACACCAAGAAATTTGAAGTTCTCAAAGTTGAATGAAAAATTGACATATTCTGTAACTTTCAGCCGGATCGACTTTGTTAGAACAAGAAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAATAAACACATAGTGAGAAGTCCAATCTCTGTAAAGCTCACCTAA

Coding sequence (CDS)

ATGAAAACTGAGCTTTGGAGTGCTTCCAACACCAATTTACAAACCTACATTGTTCATGTTAAGCAACCAGAGGTTGAGATTTTGGGTGACACCATTGATCTACAAAATTGGTACACATCATTCTTACCAGAAACGATCGAAGCGTCATCCAACGAACAATCACGGTTACTTTATTCATATCGACACGTGATCAGTGGTTTCTCTGCAAGACTTACAAAAGAACAAGTTAAAACAATGGAAGAGAAGGATGGTTTCATCTCAGCCATGCCTGAAACCACATTGAATTTGCACACAACTCACACACCTGAATATTTGGGGTTGAACCAACATTTTGGGTTATGGAAAAATTCAAACTTTGGTAAAGGAGTGATCATAGGAGTATTGGATACTGGAATTCATCCAAATCATCCTTCGTTTAACGATGAAGGAATGCCGTCACCACCGGCGAAATGGAAAGGGAGATGCGAATTTGGTGCTTCTATTTGTAACAACAAGTTGATTGGTGCAAGAACTTTCAATCTTGCTAATAATGTTTCGATTGGGAAATCACCAAATGACGAAAATGGACATGGCACACACACAGCAAGCACAGCTGCAGGCACTTTTGTTAAAGGTGCTGAAGCTTTGGGAAATGCAAGAGGCAAAGCAGTTGGAATGGCACCTTTAGCTCATATTGCAGTGTACAAAGTTTGTTCTCCAAAAGGTTGTTCCAGTAGTGATATACTTGCTGCCTTAGATGCTGCAATCGACGACGGTGTTGACGTGCTCTCGCTCTCCCTCGGTGCCCCTTCGACTCCATTTTTCAAAGACACAATTGCCGTAGGGGCATTTGCAGCCATTAAAAAGGGAATTTTTGTGAGTTGTTCAGCAGGGAATTCAGGCCCTTCCAAAAATACATTAGCCAACGAAGCACCATGGATTTTAACTGTTGGAGCTAGCACCATTGATAGAAAAATCGTGGCATTGGCAAAGCTTGAAAGTGGCAAAGTCTTTACCGGTGAATCTCTGTTCCAGCCAAGAGATTTCTCATCAAAATTCTTACCACTTGTATACGCTGGCAAAAGCGGAATCGAAGGTTCTGAATATTGTGTTGAAGGTTCACTTGAAAAGCTGAATGTGACAGGAAAAATCGTGGTATGTGAACGAGGAGGAGGAATAGGCAGAATAGCAAAAGGGTTGGTTGTGAAAAATGGTGGCGGCGCTGCCATGATTCTTGTAAACCAAAAACCAGATGGGTTTAGTACTTTAGCTGAAGCTCATGTTCTTCCAACAACCCATTTAAGCTATGAAGATGGACTTAAAATCAAAGAATATATAAATTCATCACATAATCCAAAAGCTTCAATTTCATTTGAAGGAACTCTGCTTGGAAACAGAGCCACCACTTTCTCCCCTGCTATGGCTTCTTTTTCTTCACGAGGGCCATGTCAAGCAAGCCCTGGGATCTTGAAACCTGACATAACAGGCCCTGGTGTCAACATTCTCGCAGCTTGGCCATTCCCATTAAACAACAACACAAACACGAACACAAAATCGACTTTTAATGTTATATCAGGAACATCAATGTCTTGTCCTCATTTAAGTGGAATTGCAGCTTTGATCAAGAGTAATCATCCTAATTGGTCCCCTGCTGCCATTAAATCTGCTATTATGACCTCTGCAGATGTAAGAAATCCTCAAGGGAAACCAATCGTGGATCAAGATTTGAAACCAGCGAACTTTTTCGCTATGGGGTCCGGACATGTTAATCCATCAAAAGCAGCTAACCCAGGATTGGTTTATGACATTCAGCCCGATGATTATGTTCCTTATCTTTGCCATTTGTACACAGATGCTCAAGTGTCGATTATTGTTCGTAGACAAGTAACGTGTTCGACGGTATCAAGAATACGAGAAGGCGATCTGAATTATCCTTCGTTTGCTGTGAGTTTGGGAGCTGATTCACAGGCGTTTAATAGAACAGTGACAAATGTGGGTGATGCTAATTCAGTTTATTACGCCATTGTTAAGGCACCAGCTGGAGTTTCAGTGAGAGTTACACCAAGAAATTTGAAGTTCTCAAAGTTGAATGAAAAATTGACATATTCTGTAACTTTCAGCCGGATCGACTTTGTTAGAACAAGAAGTGAATTCAGTGAAGGGTATCTCATATGGGTTTCTAATAAACACATAGTGAGAAGTCCAATCTCTGTAAAGCTCACCTAA

Protein sequence

MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISVKLT*
BLAST of Csa5G157280 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 6.0e-177
Identity = 347/748 (46.39%), Postives = 469/748 (62.70%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQ-NWYTSFLPETIEASSNEQSRLLYSYRHVIS 66
           S+S+++  TYIVH+ + ++     + DL  NWY S L      S ++ + LLY+Y + I 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMP---SSFDLHSNWYDSSL-----RSISDSAELLYTYENAIH 82

Query: 67  GFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFG-LWKNSNFGKGVI 126
           GFS RLT+E+  ++  + G IS +PE    LHTT TP +LGL++H   L+  +     V+
Sbjct: 83  GFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVV 142

Query: 127 IGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTFNLANNVSI 186
           +GVLDTG+ P   S++DEG    P+ WKG CE G    AS+CN KLIGAR F      ++
Sbjct: 143 VGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTM 202

Query: 187 G--------KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
           G        +SP D++GHGTHT+STAAG+ V+GA  LG A G A GMAP A +AVYKVC 
Sbjct: 203 GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW 262

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 306
             GC SSDILAA+D AI D V+VLS+SLG   + +++D +A+GAFAA+++GI VSCSAGN
Sbjct: 263 LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGN 322

Query: 307 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYA 366
           +GPS ++L+N APWI TVGA T+DR   ALA L +GK FTG SLF+      K LP +YA
Sbjct: 323 AGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA 382

Query: 367 GK-SGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPD 426
           G  S       C+ G+L    V GKIV+C+RG    R+ KG VVK  GG  MIL N   +
Sbjct: 383 GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAAN 442

Query: 427 GFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFS 486
           G   +A+AH+LP T +  + G  I+ Y+ +  NP ASIS  GT++G +    SP +A+FS
Sbjct: 443 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP---SPVVAAFS 502

Query: 487 SRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSG 546
           SRGP   +P ILKPD+  PGVNILAAW          + + +  FN+ISGTSMSCPH+SG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 547 IAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAA 606
           +AAL+KS HP WSPAAI+SA+MT+A      GKP++D    KP+  F  G+GHV+P+ A 
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 622

Query: 607 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSL-GA 666
           NPGL+YD+  +DY+ +LC L YT  Q+  + RR  TC         DLNYPSFAV++ G 
Sbjct: 623 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 682

Query: 667 DSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVR 726
            +  + RTVT+VG A +    +     GV + V P  L F + NEK +Y+VTF+ +D  +
Sbjct: 683 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSK 742

Query: 727 TRSEFSEGYLIWVSNKHIVRSPISVKLT 735
                S G + W   KH+V SP+++  T
Sbjct: 743 PSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Csa5G157280 vs. Swiss-Prot
Match: SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 3.9e-176
Identity = 351/754 (46.55%), Postives = 473/754 (62.73%), Query Frame = 1

Query: 14  QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETI----EASSNEQSRLLYSYRHVISGFSA 73
           QTYIV +  P  E         +W+ SFL E +    E      SRLLYSY   I GF+A
Sbjct: 26  QTYIVQL-HPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAA 85

Query: 74  RLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQ--HFGLWKNSNFGKGVIIGV 133
           +LT+ + + +      ++  P+  L + TT++ ++LGL+   + G+W  S FG+G IIGV
Sbjct: 86  QLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGV 145

Query: 134 LDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV--SIG 193
           LDTG+ P  PSF+D GMPS P KWKG C+    F +S CN KLIGAR F   + V  S  
Sbjct: 146 LDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPE 205

Query: 194 KSPN---------DENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 253
           +SPN         D  GHGTHTAST  G+ V  A  LGN  G A GMAP AHIAVYKVC 
Sbjct: 206 ESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCW 265

Query: 254 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 313
             GC SSDILAA+D AI D VDVLSLSLG    P + DTIA+G F A+++GI V C+AGN
Sbjct: 266 FNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGN 325

Query: 314 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDF--SSKFLPLV 373
           +GP ++++AN APW+ T+GA T+DR+  A+ +L +GK+  GESL+  +    + + + ++
Sbjct: 326 NGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVI 385

Query: 374 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 433
           Y    G +GSE+C+ GSL +  + GK+V+C+RG   GR  KG  VK  GG AMIL N + 
Sbjct: 386 YV-TGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 434 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 493
           +      + H+LP T + Y + + +K Y+N++  PKA I F GT++G    + +P +A F
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGR---SRAPEVAQF 505

Query: 494 SSRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLS 553
           S+RGP  A+P ILKPD+  PGVNI+AAWP  L        + +  F V+SGTSMSCPH+S
Sbjct: 506 SARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVS 565

Query: 554 GIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAA 613
           GI ALI+S +PNWSPAAIKSA+MT+AD+ + QGK I D + KPA  FA+G+GHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 614 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREG-DLNYPSFAV--SL 673
           NPGLVY+IQP DY+ YLC L +T + +  I  + V+C+ + R   G  LNYPS AV    
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685

Query: 674 GADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTF--SRI 733
           G  ++   R VTNVG  NS+Y   VKAP G+ V V P+ L F  +++ L+Y V F   + 
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745

BLAST of Csa5G157280 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.1e-170
Identity = 350/759 (46.11%), Postives = 456/759 (60.08%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISG 66
           S+S+  L++YIVHV++     L  + +  NW+ S L      SS + + LLYSY   + G
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLLRSL--PSSPQPATLLYSYSRAVHG 83

Query: 67  FSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 126
           FSARL+  Q   +      IS +P+    +HTTHTP +LG +Q+ GLW NSN+G+ VI+G
Sbjct: 84  FSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVG 143

Query: 127 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTF---------- 186
           VLDTGI P HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F          
Sbjct: 144 VLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNG 203

Query: 187 NLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
              +     +SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C 
Sbjct: 204 TKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICW 263

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPST--PFFKDTIAVGAFAAIKKGIFVSCSA 306
             GC  SDILAA+D A+ DGV V+SLS+GA  +   +  D+IA+GAF A + GI VSCSA
Sbjct: 264 TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSA 323

Query: 307 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 366
           GNSGP+  T  N APWILTVGAST+DR+  A A    GKVFTG SL+         L LV
Sbjct: 324 GNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLV 383

Query: 367 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 426
           Y+G     GS  C  G L    V GKIV+C+RGG   R+ KG  VK  GGA MIL N   
Sbjct: 384 YSGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAE 443

Query: 427 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 486
            G    A++H++P T +  + G +I++YI +S +P A ISF GTL+G   +  SP +A+F
Sbjct: 444 SGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG--PSPPSPRVAAF 503

Query: 487 SSRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTNTKSTFNVISGTSMSCP 546
           SSRGP   +P ILKPD+  PGVNILA W     P  L+ +     +  FN+ISGTSMSCP
Sbjct: 504 SSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR---RVQFNIISGTSMSCP 563

Query: 547 HLSGIAALIKSNHPNWSPAAIKSAIMTSA-DVRNPQGKPIVDQDL-KPANFFAMGSGHVN 606
           H+SG+AAL++  HP+WSPAAIKSA++T+A DV N  G+PI D    K +N F  G+GHV+
Sbjct: 564 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHVD 623

Query: 607 PSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVT----CSTVSRIREGDLNYPS 666
           P+KA NPGLVYDI+  +YV +LC +  +    ++  +  T    C T      GDLNYPS
Sbjct: 624 PNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPS 683

Query: 667 FAVSLGADSQA--FNRTVTNVG-DANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYS 726
           F+V   +  +   + R V NVG + ++VY   VK+PA V + V+P  L FSK    L Y 
Sbjct: 684 FSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYE 743

Query: 727 VTFSRIDF---VRTRSEFSEGYLIWVSNKHIVRSPISVK 733
           VTF  +     V +      G + W   +H+V+SP++V+
Sbjct: 744 VTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of Csa5G157280 vs. Swiss-Prot
Match: SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 7.4e-167
Identity = 339/754 (44.96%), Postives = 457/754 (60.61%), Query Frame = 1

Query: 5   LWSASNTNL--QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRH 64
           ++ ASN +   QTY++H      + +  ++     + S   E I         + Y Y +
Sbjct: 31  IFFASNVSSRKQTYVIHTVTTSTKHIVTSL-----FNSLQTENINDDDFSLPEIHYIYEN 90

Query: 65  VISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKG 124
            +SGFSA LT +Q+ T++   GFISA P+  L+LHTT++ E+LGL    GLW  ++    
Sbjct: 91  AMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSD 150

Query: 125 VIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV 184
           VIIG++DTGI P H SF D  M   P++W+G C+    F +S CN K+IGA  F      
Sbjct: 151 VIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYES 210

Query: 185 SIGK--------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKV 244
            +GK        S  D  GHGTHTASTAAG  V  A   G A+G A GM   + IA YK 
Sbjct: 211 IVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKA 270

Query: 245 CSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSA 304
           C   GC+S+D++AA+D AI DGVDV+SLSLG  S PF+ D IA+  F A++K IFVSCSA
Sbjct: 271 CWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSA 330

Query: 305 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 364
           GNSGP+ +T++N APW++TV AS  DR   A+ ++ + K   G SL++ +  S K LPL 
Sbjct: 331 GNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGK--SLKNLPLA 390

Query: 365 YAGKSGIE-GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQK 424
           +   +G E G+ +C+  SL++  V GKIV+C RG   GR AKG  VK  GGAAM+LV+ +
Sbjct: 391 FNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAKGEEVKRSGGAAMLLVSTE 450

Query: 425 PDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMAS 484
            +G   LA+ HVLP   L + DG  +  Y+  + N  AS+ F GT  G  A    P +A+
Sbjct: 451 AEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYGATA----PMVAA 510

Query: 485 FSSRGPCQASPGILKPDITGPGVNILAAW-PFPLNNNTNTNTKST-FNVISGTSMSCPHL 544
           FSSRGP  A P I KPDI  PG+NILA W PF   +   ++ +   FN+ISGTSM+CPH+
Sbjct: 511 FSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHI 570

Query: 545 SGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDL----KPANFFAMGSGHVN 604
           SGIAALIKS H +WSPA IKSAIMT+A + + + +PI D+        A  FA G+G+V+
Sbjct: 571 SGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVD 630

Query: 605 PSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTC-STVSRIREGDLNYPSFA 664
           P++A +PGLVYD    DY+ YLC L YT  ++ +      TC S    +  GDLNYPSFA
Sbjct: 631 PTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFA 690

Query: 665 VSL--GADSQA--FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSV 724
           V+L  GA+ +   + RTVTNVG     Y   V+ P GV VRV P+ LKF K  E+L+Y+V
Sbjct: 691 VNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTV 750

Query: 725 TFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISV 732
           T+   +  R  S  S G L+W+ +K+ VRSPI+V
Sbjct: 751 TYD-AEASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of Csa5G157280 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.8e-165
Identity = 335/743 (45.09%), Postives = 446/743 (60.03%), Query Frame = 1

Query: 11  TNLQTYIV---HVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGF 70
           T  +TYI+   H  +PE       +   +WYTS L        N +S LLY+Y     GF
Sbjct: 25  TAKKTYIIRVNHSDKPE-----SFLTHHDWYTSQL--------NSESSLLYTYTTSFHGF 84

Query: 71  SARLTKEQVKTM-EEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 130
           SA L   +  ++    +  +    +    LHTT TPE+LGLN  FG+    +   GVIIG
Sbjct: 85  SAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIG 144

Query: 131 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNVSIGK 190
           VLDTG+ P   SF+D  MP  P+KWKG CE    F + +CN KLIGAR+F+    ++ G 
Sbjct: 145 VLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGG 204

Query: 191 ---------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSP 250
                    SP D +GHGTHT++TAAG+ V+ A  LG A G A GMA  A +A YKVC  
Sbjct: 205 GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWS 264

Query: 251 KGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNS 310
            GC  SDILAA+D AI DGVDVLSLSLG  S P+++DTIA+GAF+A+++G+FVSCSAGNS
Sbjct: 265 TGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNS 324

Query: 311 GPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAG 370
           GP++ ++AN APW++TVGA T+DR   A A L +GK  TG SL+      +K L LVY  
Sbjct: 325 GPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-N 384

Query: 371 KSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGF 430
           K     S  C+ GSL+   V GKIVVC+RG    R+ KG VV++ GG  MI+ N    G 
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGE 444

Query: 431 STLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSR 490
             +A++H+LP   +  + G  ++EY+ S   P A + F+GT+L  +    SP +A+FSSR
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP---SPVVAAFSSR 504

Query: 491 GPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSGIA 550
           GP   +P ILKPD+ GPGVNILA W   +        + ++ FN++SGTSMSCPH+SG+A
Sbjct: 505 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 564

Query: 551 ALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAANP 610
            L+K+ HP WSP+AIKSA+MT+A V +    P+ D  D   +N +A GSGHV+P KA +P
Sbjct: 565 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSP 624

Query: 611 GLVYDIQPDDYVPYLCHL-YT-DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADS 670
           GLVYDI  ++Y+ +LC L YT D  V+I+ R  V CS       G LNYPSF+V  G   
Sbjct: 625 GLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK-KFSDPGQLNYPSFSVLFGGKR 684

Query: 671 QA-FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRT 730
              + R VTNVG A+SVY   V     V + V P  L F  + EK  Y+VTF     V  
Sbjct: 685 VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM 744

BLAST of Csa5G157280 vs. TrEMBL
Match: A0A0A0KMQ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1)

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 1

Query: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60
           MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY
Sbjct: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60

Query: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120
           RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG
Sbjct: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120

Query: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180
           KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180

Query: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240

Query: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300
           ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360
           ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360

Query: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420
           EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH
Sbjct: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420

Query: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480
           VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540

Query: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600
           WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660
           YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD
Sbjct: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660

Query: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720
           ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS
Sbjct: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720

Query: 721 NKHIVRSPISVKLT 735
           NKHIVRSPISVKLT
Sbjct: 721 NKHIVRSPISVKLT 734

BLAST of Csa5G157280 vs. TrEMBL
Match: A0A0A0KKE3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 1.2e-277
Identity = 490/729 (67.22%), Postives = 578/729 (79.29%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           +NL TYIVHVK+PEV       DL++W+ SFLP ++E +S EQ  LLYSYR+V+SGFSAR
Sbjct: 28  SNLHTYIVHVKKPEV-----VDDLESWHRSFLPTSLE-NSEEQPTLLYSYRNVMSGFSAR 87

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E VK MEEKDGF+SA  ET ++LHTTH+P +LGLN+ FG WK+SNFGKGVIIGVLD 
Sbjct: 88  LTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGVLDG 147

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGK------SP 190
           GI P+HPSF D GMP PPAKWKGRCEF  S CNNKLIGAR+ NLA+    GK      SP
Sbjct: 148 GITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGARSLNLASQALKGKITTLDDSP 207

Query: 191 NDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAA 250
            DE+GHGTHTASTAAGTFV GAEALGNA G AVGMAPLAH+A+YKVC  + CS+ DILA 
Sbjct: 208 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDILAG 267

Query: 251 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 310
           LDAA++DGVDVLS+SLG P  PFF D  A+GAFAAI+KGIFVSCSA NSGP   TL+NEA
Sbjct: 268 LDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLSNEA 327

Query: 311 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 370
           PWILTV ASTIDRKI A AKL +G+ F GESLFQP DF   FLPLV+ G+   E    C 
Sbjct: 328 PWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKN-ETVALCA 387

Query: 371 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 430
           EGSL+ ++V GK+VVC+RGGGI RIAKG+ VKN GGAAMIL+N + DGF+T A+AHVLP 
Sbjct: 388 EGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHVLPA 447

Query: 431 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 490
           +H+S+   LKIK YINS+  P A+I F+GT +G+    FSPA+A+FSSRGP  ASPGILK
Sbjct: 448 SHVSHTAALKIKAYINSTTYPTATIVFKGTTIGD---DFSPAIAAFSSRGPSLASPGILK 507

Query: 491 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 550
           PDITGPGV+ILAAWPFPL+N  NTNTKSTFN++SGTSMSCPHLSGIAALIKS HP+WSPA
Sbjct: 508 PDITGPGVSILAAWPFPLDN--NTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPA 567

Query: 551 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 610
           AIKS+IMT+A++ N +G PIVDQ L+PA+ FA+G+GHVNPSKA +PGLVYDIQPDDY+PY
Sbjct: 568 AIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPY 627

Query: 611 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 670
           LC L YT+ QVS+I  + + C T + I EG+LNYPSF V LG   Q F+RTVT VG    
Sbjct: 628 LCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLG-QVQTFSRTVTYVGSGRE 687

Query: 671 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 730
           VY  +++AP GVSV V PR + FS LN+K TYSVTF RI  +   +EF+EGYL WVS KH
Sbjct: 688 VYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKH 743

Query: 731 IVRSPISVK 733
           +VRSPISVK
Sbjct: 748 LVRSPISVK 743

BLAST of Csa5G157280 vs. TrEMBL
Match: B9RBY5_RICCO (Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 1.8e-273
Identity = 480/729 (65.84%), Postives = 577/729 (79.15%), Query Frame = 1

Query: 12  NLQTYIVHVKQPEVEILGDTIDLQNWYTSFLP-ETIEASSNEQSRLLYSYRHVISGFSAR 71
           NLQTYIVHV QPE        DL+NW+ SFL   T  +   +Q R+LYSY+++ISGFSAR
Sbjct: 43  NLQTYIVHVNQPEGRTFSQPEDLKNWHKSFLSFSTASSEEEQQQRMLYSYQNIISGFSAR 102

Query: 72  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 131
           LT+E+VK MEE  GF+SA  E  L L TTHTP +LGL+Q  GLWK+S+FGKGVIIG+LD 
Sbjct: 103 LTQEEVKAMEEITGFVSACLERKLRLQTTHTPSFLGLHQQMGLWKDSDFGKGVIIGILDG 162

Query: 132 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIG---KSPNDE 191
           G++P+HPSF+DEGMP PPAKWKGRCEF AS CNNKLIGARTFNLA     G   + P D 
Sbjct: 163 GVYPSHPSFSDEGMPLPPAKWKGRCEFNASECNNKLIGARTFNLAAKTMKGAPTEPPIDV 222

Query: 192 NGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVC--SPKG-CSSSDILAA 251
           +GHGTHTASTAAG FV  ++ LGNA+G AVGMAP AH+A+YKVC   P   C  SD+LA 
Sbjct: 223 DGHGTHTASTAAGGFVYNSDVLGNAKGTAVGMAPFAHLAIYKVCFGDPNDDCPESDVLAG 282

Query: 252 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 311
           LDAA+DDGVDVLSLSLG  S PFF+D IA+G+FAAI+KGIFVSCSAGNSGPSK+TL+NEA
Sbjct: 283 LDAAVDDGVDVLSLSLGDVSMPFFQDNIAIGSFAAIQKGIFVSCSAGNSGPSKSTLSNEA 342

Query: 312 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 371
           PWILTVGASTIDR+IVA+AKL +G+   GES+ QP +F +  LP+VYAG +    S +C 
Sbjct: 343 PWILTVGASTIDRRIVAIAKLGNGEELDGESVSQPSNFPTTLLPIVYAGMNSKPDSAFCG 402

Query: 372 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 431
           EG+LE +NV  K+V+CERGGGIGRIAKG  VKN GGAAMILVN + +GFST+A+AHVLP 
Sbjct: 403 EGALEGMNVKDKVVMCERGGGIGRIAKGDEVKNAGGAAMILVNDETNGFSTIADAHVLPA 462

Query: 432 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 491
           TH+S+  GLKIK YINS+  P A+I F+GT++G+ +   SPA+ SFSSRGP  ASPGILK
Sbjct: 463 THVSFAAGLKIKAYINSTKTPMATILFKGTVIGDSS---SPAVTSFSSRGPSLASPGILK 522

Query: 492 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 551
           PDI GPGV+ILAAWPFPL+N  NTNTK TFN++SGTSMSCPHLSGIAAL+KS+HP WSPA
Sbjct: 523 PDIIGPGVSILAAWPFPLDN--NTNTKLTFNIMSGTSMSCPHLSGIAALLKSSHPYWSPA 582

Query: 552 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 611
           AIKSAI+T+AD+ N +GKPIVD+  +PA+FFA G+GHVNPS+A +PGLVYDIQPDDY+PY
Sbjct: 583 AIKSAIVTTADILNMEGKPIVDETHQPADFFATGAGHVNPSRANDPGLVYDIQPDDYIPY 642

Query: 612 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 671
           LC L YTD QVSII  R ++CST+  I EG LNYPSF+V+LG   Q F RTVTNVG ANS
Sbjct: 643 LCGLNYTDEQVSIIAHRPISCSTIQTIAEGQLNYPSFSVTLG-PPQTFIRTVTNVGYANS 702

Query: 672 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 731
           V+ A + +P GV+V V P  L FSKLN+K TYS+TFS   +    SEF +GY+ WVS+K+
Sbjct: 703 VFAATITSPPGVAVSVKPSRLYFSKLNQKATYSITFSHTGYGAKTSEFGQGYITWVSDKY 762

Query: 732 IVRSPISVK 733
            V SPISV+
Sbjct: 763 FVGSPISVR 765

BLAST of Csa5G157280 vs. TrEMBL
Match: B9GW37_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2)

HSP 1 Score: 939.1 bits (2426), Expect = 3.2e-270
Identity = 480/728 (65.93%), Postives = 567/728 (77.88%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           T L  YIVHV +PE   + +  DL++WY SFLP +  ASS +Q R+LY+Y++V+SGF+AR
Sbjct: 33  TTLLNYIVHVAKPEGRTMAEFEDLESWYQSFLPVST-ASSEKQQRMLYAYQNVMSGFAAR 92

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E+VK+MEEKDGF+SA PE  L+L TTHTP +LGL+Q  G WK SNFGKGVIIGVLD 
Sbjct: 93  LTQEEVKSMEEKDGFLSARPERILHLQTTHTPRFLGLHQELGFWKESNFGKGVIIGVLDG 152

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKS----PND 190
           GI P+HPSF+DEGMP PPAKWKGRC+F AS CNNKLIGAR+FN+A     G +    P D
Sbjct: 153 GIFPSHPSFSDEGMPPPPAKWKGRCDFNASDCNNKLIGARSFNIAAKAKKGSAATEPPID 212

Query: 191 ENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVC---SPKGCSSSDILA 250
            +GHGTHTASTAAG FVK AE LGNARG AVG+AP AH+A+YKVC       C  SDILA
Sbjct: 213 VDGHGTHTASTAAGAFVKDAEVLGNARGTAVGIAPHAHLAIYKVCFGDPGDDCPESDILA 272

Query: 251 ALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANE 310
            LDAA+ DGVDVLSLSLG  S P F DTIA+G+FAAI+KGIFVSCSAGNSGP   TL+NE
Sbjct: 273 GLDAAVQDGVDVLSLSLGEDSVPLFNDTIAIGSFAAIQKGIFVSCSAGNSGPFNGTLSNE 332

Query: 311 APWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYC 370
           APWILTVGAST+DR+  A A+L +G+   GESL Q  +F S  LPLVYAG SG   S  C
Sbjct: 333 APWILTVGASTVDRRFSATARLGNGEQIDGESLSQHSNFPSTLLPLVYAGMSGKPNSSLC 392

Query: 371 VEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLP 430
            EG+LE ++V GKIV+CERGGGIGRIAKG  VKN GGAAMIL+N++ DGFST A+ HVLP
Sbjct: 393 GEGALEGMDVKGKIVLCERGGGIGRIAKGGEVKNAGGAAMILMNEEADGFSTNADVHVLP 452

Query: 431 TTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGIL 490
            TH+S+  GLKIK YINS+  P A+I F+GT++G+ +   SP +ASFSSRGP  ASPGIL
Sbjct: 453 ATHVSFAAGLKIKAYINSTQAPMATILFKGTVIGDSS---SPFVASFSSRGPSLASPGIL 512

Query: 491 KPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSP 550
           KPDI GPGV+ILAAWPFPL+N  NTN+KSTFN+ISGTSMSCPHLSGIAAL+KS+HP WSP
Sbjct: 513 KPDIIGPGVSILAAWPFPLDN--NTNSKSTFNIISGTSMSCPHLSGIAALLKSSHPYWSP 572

Query: 551 AAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVP 610
           AAIKSAIMT+AD  N +GK IVDQ L+PA+ FA G+GHVNPS+A NPGLVYDIQPDDY+P
Sbjct: 573 AAIKSAIMTTADTLNMEGKLIVDQTLQPADIFATGAGHVNPSRANNPGLVYDIQPDDYIP 632

Query: 611 YLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDAN 670
           YLC L Y D +VSIIV  QV CS    I EG+LNYPSFAV+LG  SQ F RTVTNVGD N
Sbjct: 633 YLCGLGYADNEVSIIVHEQVKCSEKPSIPEGELNYPSFAVTLG-PSQTFTRTVTNVGDVN 692

Query: 671 SVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNK 730
           S Y   + +P GV V V P  L FSK+N+K TYSV FSR ++    SE ++GY++W S K
Sbjct: 693 SAYEVAIVSPPGVDVTVKPSKLYFSKVNQKATYSVAFSRTEYGGKTSETAQGYIVWASAK 752

BLAST of Csa5G157280 vs. TrEMBL
Match: A0A067JNI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 6.3e-266
Identity = 466/730 (63.84%), Postives = 566/730 (77.53%), Query Frame = 1

Query: 10  NTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSA 69
           N NLQ YIVHV  PE        +L+NW+ SFLP +  +S  +Q R+LYSY ++ISGFSA
Sbjct: 32  NKNLQAYIVHVSPPEGRTFSQRENLENWHKSFLPFSTASSEKQQKRMLYSYHNIISGFSA 91

Query: 70  RLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLD 129
           RLT E+VK MEE +GF+ A PE  L+L TTHTP +LGL++  G WK SNFGKGVIIGVLD
Sbjct: 92  RLTHEEVKAMEEINGFVLARPERKLHLQTTHTPSFLGLHRQMGFWKESNFGKGVIIGVLD 151

Query: 130 TGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIG---KSPND 189
            G+ P+HPSFND+GMP PPAKWKGRCEF AS CNNKLIGAR+FNLA     G   ++P D
Sbjct: 152 GGVFPSHPSFNDKGMPPPPAKWKGRCEFNASKCNNKLIGARSFNLAAKAMKGIAAETPID 211

Query: 190 ENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVC--SPKG-CSSSDILA 249
            +GHGTHTASTAAG+FV  A  LGNA+G AVGMAP AH+A+YKVC   P   C  SDILA
Sbjct: 212 VDGHGTHTASTAAGSFVYNANVLGNAKGTAVGMAPYAHLAIYKVCFGDPNDDCPESDILA 271

Query: 250 ALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANE 309
            LDAAI DGVDVLSLS+G  S PFF+D IA+G+FAAI+KGIFVSC+AGNSGP   TL+NE
Sbjct: 272 GLDAAIQDGVDVLSLSIGDISMPFFQDNIAIGSFAAIQKGIFVSCAAGNSGPFNGTLSNE 331

Query: 310 APWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYC 369
           APWILTVGASTIDRKI A AKL +G+   GES+ QP +F +  LPLVY G +G   S +C
Sbjct: 332 APWILTVGASTIDRKIAATAKLGNGEELDGESVLQPSNFPTTLLPLVYPGMNGKTESAFC 391

Query: 370 VEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLP 429
            E +++ ++V  K+V+CERGGGIGR+AKG  VKN GGAAMIL+N +  GFST+A+AHVLP
Sbjct: 392 SERAVQGMDVKDKVVLCERGGGIGRVAKGEEVKNAGGAAMILINDEISGFSTIADAHVLP 451

Query: 430 TTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGIL 489
            TH+S+  GL+IK YINS+  P A+I F+GT++G+     SPA+ SFSSRGP  ASPGIL
Sbjct: 452 ATHVSFAAGLQIKAYINSTKTPMATILFKGTVIGD---PLSPAVTSFSSRGPNLASPGIL 511

Query: 490 KPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSP 549
           KPDI GPGV+ILAAWPFPL+N   TNTKSTFN++SGTSM+CPHLSGIAAL+KS+HP WSP
Sbjct: 512 KPDIIGPGVSILAAWPFPLDN--TTNTKSTFNLVSGTSMACPHLSGIAALLKSSHPYWSP 571

Query: 550 AAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVP 609
           AAIKSAIMT+AD+ N +G PIVD+  +PA+ F +G+GHVNPS+A +PGL+YDIQPDDY+P
Sbjct: 572 AAIKSAIMTTADIFNMEGSPIVDEKHQPADLFTIGAGHVNPSRANDPGLIYDIQPDDYIP 631

Query: 610 YLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDAN 669
           YLC L Y + QVSII  R++ CS    I EG LNYPSF+V+LGA SQ F RTVTNVG+AN
Sbjct: 632 YLCGLGYKEEQVSIIAHRRIKCSEKLSIPEGQLNYPSFSVTLGA-SQTFTRTVTNVGEAN 691

Query: 670 SVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNK 729
           SVY A +  P GV+V V P  L FS++N+K+TYSVTFS        SEF++GY++W S K
Sbjct: 692 SVYAATIVPPPGVAVTVEPYRLYFSQVNQKVTYSVTFSPTGSSGKTSEFAQGYILWSSAK 751

Query: 730 HIVRSPISVK 733
           H+VRSPISV+
Sbjct: 752 HLVRSPISVR 755

BLAST of Csa5G157280 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 622.5 bits (1604), Expect = 3.4e-178
Identity = 347/748 (46.39%), Postives = 469/748 (62.70%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQ-NWYTSFLPETIEASSNEQSRLLYSYRHVIS 66
           S+S+++  TYIVH+ + ++     + DL  NWY S L      S ++ + LLY+Y + I 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMP---SSFDLHSNWYDSSL-----RSISDSAELLYTYENAIH 82

Query: 67  GFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFG-LWKNSNFGKGVI 126
           GFS RLT+E+  ++  + G IS +PE    LHTT TP +LGL++H   L+  +     V+
Sbjct: 83  GFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVV 142

Query: 127 IGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTFNLANNVSI 186
           +GVLDTG+ P   S++DEG    P+ WKG CE G    AS+CN KLIGAR F      ++
Sbjct: 143 VGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTM 202

Query: 187 G--------KSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
           G        +SP D++GHGTHT+STAAG+ V+GA  LG A G A GMAP A +AVYKVC 
Sbjct: 203 GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW 262

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 306
             GC SSDILAA+D AI D V+VLS+SLG   + +++D +A+GAFAA+++GI VSCSAGN
Sbjct: 263 LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGN 322

Query: 307 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYA 366
           +GPS ++L+N APWI TVGA T+DR   ALA L +GK FTG SLF+      K LP +YA
Sbjct: 323 AGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYA 382

Query: 367 GK-SGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPD 426
           G  S       C+ G+L    V GKIV+C+RG    R+ KG VVK  GG  MIL N   +
Sbjct: 383 GNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAAN 442

Query: 427 GFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFS 486
           G   +A+AH+LP T +  + G  I+ Y+ +  NP ASIS  GT++G +    SP +A+FS
Sbjct: 443 GEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP---SPVVAAFS 502

Query: 487 SRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSG 546
           SRGP   +P ILKPD+  PGVNILAAW          + + +  FN+ISGTSMSCPH+SG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 547 IAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAA 606
           +AAL+KS HP WSPAAI+SA+MT+A      GKP++D    KP+  F  G+GHV+P+ A 
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTAT 622

Query: 607 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSL-GA 666
           NPGL+YD+  +DY+ +LC L YT  Q+  + RR  TC         DLNYPSFAV++ G 
Sbjct: 623 NPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV 682

Query: 667 DSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVR 726
            +  + RTVT+VG A +    +     GV + V P  L F + NEK +Y+VTF+ +D  +
Sbjct: 683 GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFT-VDSSK 742

Query: 727 TRSEFSEGYLIWVSNKHIVRSPISVKLT 735
                S G + W   KH+V SP+++  T
Sbjct: 743 PSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Csa5G157280 vs. TAIR10
Match: AT1G04110.1 (AT1G04110.1 Subtilase family protein)

HSP 1 Score: 619.8 bits (1597), Expect = 2.2e-177
Identity = 351/754 (46.55%), Postives = 473/754 (62.73%), Query Frame = 1

Query: 14  QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETI----EASSNEQSRLLYSYRHVISGFSA 73
           QTYIV +  P  E         +W+ SFL E +    E      SRLLYSY   I GF+A
Sbjct: 26  QTYIVQL-HPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAA 85

Query: 74  RLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQ--HFGLWKNSNFGKGVIIGV 133
           +LT+ + + +      ++  P+  L + TT++ ++LGL+   + G+W  S FG+G IIGV
Sbjct: 86  QLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGV 145

Query: 134 LDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV--SIG 193
           LDTG+ P  PSF+D GMPS P KWKG C+    F +S CN KLIGAR F   + V  S  
Sbjct: 146 LDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPE 205

Query: 194 KSPN---------DENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 253
           +SPN         D  GHGTHTAST  G+ V  A  LGN  G A GMAP AHIAVYKVC 
Sbjct: 206 ESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCW 265

Query: 254 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGN 313
             GC SSDILAA+D AI D VDVLSLSLG    P + DTIA+G F A+++GI V C+AGN
Sbjct: 266 FNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGN 325

Query: 314 SGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDF--SSKFLPLV 373
           +GP ++++AN APW+ T+GA T+DR+  A+ +L +GK+  GESL+  +    + + + ++
Sbjct: 326 NGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVI 385

Query: 374 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 433
           Y    G +GSE+C+ GSL +  + GK+V+C+RG   GR  KG  VK  GG AMIL N + 
Sbjct: 386 YV-TGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 434 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 493
           +      + H+LP T + Y + + +K Y+N++  PKA I F GT++G    + +P +A F
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGR---SRAPEVAQF 505

Query: 494 SSRGPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLS 553
           S+RGP  A+P ILKPD+  PGVNI+AAWP  L        + +  F V+SGTSMSCPH+S
Sbjct: 506 SARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVS 565

Query: 554 GIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAA 613
           GI ALI+S +PNWSPAAIKSA+MT+AD+ + QGK I D + KPA  FA+G+GHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 614 NPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTCSTVSRIREG-DLNYPSFAV--SL 673
           NPGLVY+IQP DY+ YLC L +T + +  I  + V+C+ + R   G  LNYPS AV    
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685

Query: 674 GADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTF--SRI 733
           G  ++   R VTNVG  NS+Y   VKAP G+ V V P+ L F  +++ L+Y V F   + 
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745

BLAST of Csa5G157280 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 601.7 bits (1550), Expect = 6.2e-172
Identity = 350/759 (46.11%), Postives = 456/759 (60.08%), Query Frame = 1

Query: 7   SASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISG 66
           S+S+  L++YIVHV++     L  + +  NW+ S L      SS + + LLYSY   + G
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLLRSL--PSSPQPATLLYSYSRAVHG 83

Query: 67  FSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 126
           FSARL+  Q   +      IS +P+    +HTTHTP +LG +Q+ GLW NSN+G+ VI+G
Sbjct: 84  FSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVG 143

Query: 127 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFG----ASICNNKLIGARTF---------- 186
           VLDTGI P HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F          
Sbjct: 144 VLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNG 203

Query: 187 NLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCS 246
              +     +SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A IA YK+C 
Sbjct: 204 TKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICW 263

Query: 247 PKGCSSSDILAALDAAIDDGVDVLSLSLGAPST--PFFKDTIAVGAFAAIKKGIFVSCSA 306
             GC  SDILAA+D A+ DGV V+SLS+GA  +   +  D+IA+GAF A + GI VSCSA
Sbjct: 264 TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSA 323

Query: 307 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 366
           GNSGP+  T  N APWILTVGAST+DR+  A A    GKVFTG SL+         L LV
Sbjct: 324 GNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLV 383

Query: 367 YAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKP 426
           Y+G     GS  C  G L    V GKIV+C+RGG   R+ KG  VK  GGA MIL N   
Sbjct: 384 YSGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAE 443

Query: 427 DGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASF 486
            G    A++H++P T +  + G +I++YI +S +P A ISF GTL+G   +  SP +A+F
Sbjct: 444 SGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG--PSPPSPRVAAF 503

Query: 487 SSRGPCQASPGILKPDITGPGVNILAAW-----PFPLNNNTNTNTKSTFNVISGTSMSCP 546
           SSRGP   +P ILKPD+  PGVNILA W     P  L+ +     +  FN+ISGTSMSCP
Sbjct: 504 SSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR---RVQFNIISGTSMSCP 563

Query: 547 HLSGIAALIKSNHPNWSPAAIKSAIMTSA-DVRNPQGKPIVDQDL-KPANFFAMGSGHVN 606
           H+SG+AAL++  HP+WSPAAIKSA++T+A DV N  G+PI D    K +N F  G+GHV+
Sbjct: 564 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHVD 623

Query: 607 PSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVT----CSTVSRIREGDLNYPS 666
           P+KA NPGLVYDI+  +YV +LC +  +    ++  +  T    C T      GDLNYPS
Sbjct: 624 PNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPS 683

Query: 667 FAVSLGADSQA--FNRTVTNVG-DANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYS 726
           F+V   +  +   + R V NVG + ++VY   VK+PA V + V+P  L FSK    L Y 
Sbjct: 684 FSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYE 743

Query: 727 VTFSRIDF---VRTRSEFSEGYLIWVSNKHIVRSPISVK 733
           VTF  +     V +      G + W   +H+V+SP++V+
Sbjct: 744 VTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of Csa5G157280 vs. TAIR10
Match: AT1G01900.1 (AT1G01900.1 subtilase family protein)

HSP 1 Score: 589.0 bits (1517), Expect = 4.2e-168
Identity = 339/754 (44.96%), Postives = 457/754 (60.61%), Query Frame = 1

Query: 5   LWSASNTNL--QTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRH 64
           ++ ASN +   QTY++H      + +  ++     + S   E I         + Y Y +
Sbjct: 31  IFFASNVSSRKQTYVIHTVTTSTKHIVTSL-----FNSLQTENINDDDFSLPEIHYIYEN 90

Query: 65  VISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKG 124
            +SGFSA LT +Q+ T++   GFISA P+  L+LHTT++ E+LGL    GLW  ++    
Sbjct: 91  AMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSD 150

Query: 125 VIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNV 184
           VIIG++DTGI P H SF D  M   P++W+G C+    F +S CN K+IGA  F      
Sbjct: 151 VIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYES 210

Query: 185 SIGK--------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKV 244
            +GK        S  D  GHGTHTASTAAG  V  A   G A+G A GM   + IA YK 
Sbjct: 211 IVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKA 270

Query: 245 CSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSA 304
           C   GC+S+D++AA+D AI DGVDV+SLSLG  S PF+ D IA+  F A++K IFVSCSA
Sbjct: 271 CWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSA 330

Query: 305 GNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLV 364
           GNSGP+ +T++N APW++TV AS  DR   A+ ++ + K   G SL++ +  S K LPL 
Sbjct: 331 GNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGK--SLKNLPLA 390

Query: 365 YAGKSGIE-GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQK 424
           +   +G E G+ +C+  SL++  V GKIV+C RG   GR AKG  VK  GGAAM+LV+ +
Sbjct: 391 FNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAKGEEVKRSGGAAMLLVSTE 450

Query: 425 PDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMAS 484
            +G   LA+ HVLP   L + DG  +  Y+  + N  AS+ F GT  G  A    P +A+
Sbjct: 451 AEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYGATA----PMVAA 510

Query: 485 FSSRGPCQASPGILKPDITGPGVNILAAW-PFPLNNNTNTNTKST-FNVISGTSMSCPHL 544
           FSSRGP  A P I KPDI  PG+NILA W PF   +   ++ +   FN+ISGTSM+CPH+
Sbjct: 511 FSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHI 570

Query: 545 SGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDL----KPANFFAMGSGHVN 604
           SGIAALIKS H +WSPA IKSAIMT+A + + + +PI D+        A  FA G+G+V+
Sbjct: 571 SGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVD 630

Query: 605 PSKAANPGLVYDIQPDDYVPYLCHL-YTDAQVSIIVRRQVTC-STVSRIREGDLNYPSFA 664
           P++A +PGLVYD    DY+ YLC L YT  ++ +      TC S    +  GDLNYPSFA
Sbjct: 631 PTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFA 690

Query: 665 VSL--GADSQA--FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSV 724
           V+L  GA+ +   + RTVTNVG     Y   V+ P GV VRV P+ LKF K  E+L+Y+V
Sbjct: 691 VNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTV 750

Query: 725 TFSRIDFVRTRSEFSEGYLIWVSNKHIVRSPISV 732
           T+   +  R  S  S G L+W+ +K+ VRSPI+V
Sbjct: 751 TYD-AEASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of Csa5G157280 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 584.3 bits (1505), Expect = 1.0e-166
Identity = 335/743 (45.09%), Postives = 446/743 (60.03%), Query Frame = 1

Query: 11  TNLQTYIV---HVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGF 70
           T  +TYI+   H  +PE       +   +WYTS L        N +S LLY+Y     GF
Sbjct: 25  TAKKTYIIRVNHSDKPE-----SFLTHHDWYTSQL--------NSESSLLYTYTTSFHGF 84

Query: 71  SARLTKEQVKTM-EEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIG 130
           SA L   +  ++    +  +    +    LHTT TPE+LGLN  FG+    +   GVIIG
Sbjct: 85  SAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIG 144

Query: 131 VLDTGIHPNHPSFNDEGMPSPPAKWKGRCE----FGASICNNKLIGARTFNLANNVSIGK 190
           VLDTG+ P   SF+D  MP  P+KWKG CE    F + +CN KLIGAR+F+    ++ G 
Sbjct: 145 VLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGG 204

Query: 191 ---------SPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSP 250
                    SP D +GHGTHT++TAAG+ V+ A  LG A G A GMA  A +A YKVC  
Sbjct: 205 GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWS 264

Query: 251 KGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNS 310
            GC  SDILAA+D AI DGVDVLSLSLG  S P+++DTIA+GAF+A+++G+FVSCSAGNS
Sbjct: 265 TGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNS 324

Query: 311 GPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAG 370
           GP++ ++AN APW++TVGA T+DR   A A L +GK  TG SL+      +K L LVY  
Sbjct: 325 GPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-N 384

Query: 371 KSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGF 430
           K     S  C+ GSL+   V GKIVVC+RG    R+ KG VV++ GG  MI+ N    G 
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGE 444

Query: 431 STLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSR 490
             +A++H+LP   +  + G  ++EY+ S   P A + F+GT+L  +    SP +A+FSSR
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP---SPVVAAFSSR 504

Query: 491 GPCQASPGILKPDITGPGVNILAAWPFPLNNN--TNTNTKSTFNVISGTSMSCPHLSGIA 550
           GP   +P ILKPD+ GPGVNILA W   +        + ++ FN++SGTSMSCPH+SG+A
Sbjct: 505 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 564

Query: 551 ALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVD-QDLKPANFFAMGSGHVNPSKAANP 610
            L+K+ HP WSP+AIKSA+MT+A V +    P+ D  D   +N +A GSGHV+P KA +P
Sbjct: 565 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSP 624

Query: 611 GLVYDIQPDDYVPYLCHL-YT-DAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADS 670
           GLVYDI  ++Y+ +LC L YT D  V+I+ R  V CS       G LNYPSF+V  G   
Sbjct: 625 GLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK-KFSDPGQLNYPSFSVLFGGKR 684

Query: 671 QA-FNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRT 730
              + R VTNVG A+SVY   V     V + V P  L F  + EK  Y+VTF     V  
Sbjct: 685 VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSM 744

BLAST of Csa5G157280 vs. NCBI nr
Match: gi|449459730|ref|XP_004147599.1| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1473.8 bits (3814), Expect = 0.0e+00
Identity = 734/734 (100.00%), Postives = 734/734 (100.00%), Query Frame = 1

Query: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60
           MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY
Sbjct: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60

Query: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120
           RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG
Sbjct: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120

Query: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180
           KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI
Sbjct: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180

Query: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD
Sbjct: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240

Query: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300
           ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300

Query: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360
           ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS
Sbjct: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360

Query: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420
           EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH
Sbjct: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420

Query: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480
           VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480

Query: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN
Sbjct: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540

Query: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600
           WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600

Query: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660
           YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD
Sbjct: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660

Query: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720
           ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS
Sbjct: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720

Query: 721 NKHIVRSPISVKLT 735
           NKHIVRSPISVKLT
Sbjct: 721 NKHIVRSPISVKLT 734

BLAST of Csa5G157280 vs. NCBI nr
Match: gi|659073650|ref|XP_008437177.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 688/734 (93.73%), Postives = 712/734 (97.00%), Query Frame = 1

Query: 1   MKTELWSASNTNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSY 60
           MKTEL S SNTNLQTYIVHVKQP +EILGDTIDLQNWYTSFLPETIEASS+EQ RLLYSY
Sbjct: 21  MKTELSSTSNTNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYSY 80

Query: 61  RHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFG 120
           RHV+SGFSARLTKEQVK MEEKDGFISAMPETTLNLHTTHTPEYLGLN+HFGLWKNSNFG
Sbjct: 81  RHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNFG 140

Query: 121 KGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSI 180
           KGVIIGVLDTGIHPNHPSFNDEGM SPPAKWKGRCEFGASICNNKLIGARTFNLANN  I
Sbjct: 141 KGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVI 200

Query: 181 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSD 240
           GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIA+YKVCSP GCSSSD
Sbjct: 201 GKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSD 260

Query: 241 ILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTL 300
           ILAALDAAIDDGVDVLSLSLGAPSTPFF+DTIA+GAFAAIKKGIFVSCSAGNSGPSKNTL
Sbjct: 261 ILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNTL 320

Query: 301 ANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGS 360
           ANEAPWILTVGASTIDRKIVALAKL SG+V+TGESLFQPR+F SKFLPLVYAGKSGIEGS
Sbjct: 321 ANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGS 380

Query: 361 EYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAH 420
           EYC++GSLEKLNVTGK+VVCERGGGI R+AKGLVVKNGGGAAMIL+NQKP+GFSTLAEAH
Sbjct: 381 EYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAH 440

Query: 421 VLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASP 480
           VLPTTHLSYEDGLKIK YINSSHNPKASISF+GTLLGNRATTFSPAMASFSSRGPCQASP
Sbjct: 441 VLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASP 500

Query: 481 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPN 540
           GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKS HPN
Sbjct: 501 GILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPN 560

Query: 541 WSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 600
           WSPAAIKSAIMTSADVRNPQGKPI+DQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD
Sbjct: 561 WSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDD 620

Query: 601 YVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGD 660
           Y+PYLCHLYTDAQVSIIVR+QVTCSTV RIREGDLNYPSFAVSLGA SQ FNRTVTNVGD
Sbjct: 621 YIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGD 680

Query: 661 ANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVS 720
           ANSVYY IV+APAGVSV+VTP NLKFSKLNEK+TYSVTFSRIDFVRT SEFSEGYLIWVS
Sbjct: 681 ANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWVS 740

Query: 721 NKHIVRSPISVKLT 735
            K +VRSPISVKLT
Sbjct: 741 KKLMVRSPISVKLT 754

BLAST of Csa5G157280 vs. NCBI nr
Match: gi|659074655|ref|XP_008437722.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1061.6 bits (2744), Expect = 6.2e-307
Identity = 521/726 (71.76%), Postives = 618/726 (85.12%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTID--LQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFS 70
           + LQTYIVHV+QP++ ++G++ +  +++WY SF+P++ E +  EQ +LLYSYR+V+SGFS
Sbjct: 41  SKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTV-EQPQLLYSYRNVMSGFS 100

Query: 71  ARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVL 130
           ARLT EQVK ME+KDGFISAMPET ++LHTTHTPEYLGLNQ FG+WKNSNFGKGVI+GVL
Sbjct: 101 ARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVL 160

Query: 131 DTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDEN 190
           DTGIHPNHPSFNDEGM  PPAKWKG+CEF +S+CNNKLIGARTFNL NN  + +SPNDE 
Sbjct: 161 DTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEK 220

Query: 191 GHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAA 250
           GHGTHTASTAAG FV+ AEALGNA+GKA G+APLAH+A+YKVCS K C SSD+ A +DAA
Sbjct: 221 GHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAA 280

Query: 251 IDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWIL 310
           IDDGVDVLS+SLG+ S PFFKD IAV  F AI+KGIFVS SAGNSGP  +TL+N+APWIL
Sbjct: 281 IDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWIL 340

Query: 311 TVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSL 370
           TVGASTI+R+IVA+AKL +G+ + GESL+QP DF SKFLPLVYAG    +   +C EGSL
Sbjct: 341 TVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSL 400

Query: 371 EKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLS 430
           E ++V GK+VVCE  GG+GR+AKGLVVKN GGAAMIL+NQ+ DGFSTL+EAHVLP TH+S
Sbjct: 401 ENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVS 460

Query: 431 YEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDIT 490
           Y+ G+ IK YINSS NP ASISF+GT++G+     +P+MASFSSRGPC  SPGILKPDIT
Sbjct: 461 YKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDIT 520

Query: 491 GPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKS 550
           GPGVNILAAWPFPL+N  +TNTKSTFNVISGTSMSCPHLSGIAALIKS HPNWSPAAIKS
Sbjct: 521 GPGVNILAAWPFPLDN--DTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKS 580

Query: 551 AIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHL 610
           AIMT+A+++ PQG+PI DQDL+PANFFAMG+GHVNPSKAA+PGLVYDIQPDDY+PYLC L
Sbjct: 581 AIMTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGL 640

Query: 611 YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAI 670
           Y D +VSIIV R V C  V  IREGDLNYPSF+V+LG   Q F RTVTNVG+ANSVY AI
Sbjct: 641 YKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGG-LQTFKRTVTNVGEANSVYTAI 700

Query: 671 VKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWV--SNKHIVR 730
           V+AP GVS+ V PR L FS++N+ +T++VTF+RI +V+   EF EGYL WV  S K++VR
Sbjct: 701 VEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKWVSKSKKYVVR 760

Query: 731 SPISVK 733
           SP+SVK
Sbjct: 761 SPVSVK 762

BLAST of Csa5G157280 vs. NCBI nr
Match: gi|449459724|ref|XP_004147596.1| (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus])

HSP 1 Score: 963.8 bits (2490), Expect = 1.8e-277
Identity = 490/729 (67.22%), Postives = 578/729 (79.29%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           +NL TYIVHVK+PEV       DL++W+ SFLP ++E +S EQ  LLYSYR+V+SGFSAR
Sbjct: 28  SNLHTYIVHVKKPEV-----VDDLESWHRSFLPTSLE-NSEEQPTLLYSYRNVMSGFSAR 87

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E VK MEEKDGF+SA  ET ++LHTTH+P +LGLN+ FG WK+SNFGKGVIIGVLD 
Sbjct: 88  LTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNRQFGFWKDSNFGKGVIIGVLDG 147

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGK------SP 190
           GI P+HPSF D GMP PPAKWKGRCEF  S CNNKLIGAR+ NLA+    GK      SP
Sbjct: 148 GITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGARSLNLASQALKGKITTLDDSP 207

Query: 191 NDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAA 250
            DE+GHGTHTASTAAGTFV GAEALGNA G AVGMAPLAH+A+YKVC  + CS+ DILA 
Sbjct: 208 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGESCSNVDILAG 267

Query: 251 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 310
           LDAA++DGVDVLS+SLG P  PFF D  A+GAFAAI+KGIFVSCSA NSGP   TL+NEA
Sbjct: 268 LDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKGIFVSCSAANSGPFNATLSNEA 327

Query: 311 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 370
           PWILTV ASTIDRKI A AKL +G+ F GESLFQP DF   FLPLV+ G+   E    C 
Sbjct: 328 PWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKN-ETVALCA 387

Query: 371 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 430
           EGSL+ ++V GK+VVC+RGGGI RIAKG+ VKN GGAAMIL+N + DGF+T A+AHVLP 
Sbjct: 388 EGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMILLNAESDGFTTEADAHVLPA 447

Query: 431 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 490
           +H+S+   LKIK YINS+  P A+I F+GT +G+    FSPA+A+FSSRGP  ASPGILK
Sbjct: 448 SHVSHTAALKIKAYINSTTYPTATIVFKGTTIGD---DFSPAIAAFSSRGPSLASPGILK 507

Query: 491 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 550
           PDITGPGV+ILAAWPFPL+N  NTNTKSTFN++SGTSMSCPHLSGIAALIKS HP+WSPA
Sbjct: 508 PDITGPGVSILAAWPFPLDN--NTNTKSTFNIVSGTSMSCPHLSGIAALIKSAHPDWSPA 567

Query: 551 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 610
           AIKS+IMT+A++ N +G PIVDQ L+PA+ FA+G+GHVNPSKA +PGLVYDIQPDDY+PY
Sbjct: 568 AIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVDPGLVYDIQPDDYIPY 627

Query: 611 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 670
           LC L YT+ QVS+I  + + C T + I EG+LNYPSF V LG   Q F+RTVT VG    
Sbjct: 628 LCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLG-QVQTFSRTVTYVGSGRE 687

Query: 671 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 730
           VY  +++AP GVSV V PR + FS LN+K TYSVTF RI  +   +EF+EGYL WVS KH
Sbjct: 688 VYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPSTEFAEGYLKWVSAKH 743

Query: 731 IVRSPISVK 733
           +VRSPISVK
Sbjct: 748 LVRSPISVK 743

BLAST of Csa5G157280 vs. NCBI nr
Match: gi|659073656|ref|XP_008437181.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 961.1 bits (2483), Expect = 1.1e-276
Identity = 490/729 (67.22%), Postives = 575/729 (78.88%), Query Frame = 1

Query: 11  TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSAR 70
           +NL TYIVHVK+PEV       DL+ W+ SFLP +++ +  EQ  LLYSYR+V+SGFSAR
Sbjct: 28  SNLHTYIVHVKKPEV-----VDDLEIWHRSFLPTSLD-NEEEQPTLLYSYRNVMSGFSAR 87

Query: 71  LTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDT 130
           LT+E VK MEEKDGF+SA  ET ++LHTTHTP++LGLN+ FG WK+SNFGKGVIIGVLD 
Sbjct: 88  LTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDG 147

Query: 131 GIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGK------SP 190
           GI PNHPSF+D GM  PPAKWKGRCEF  S CNNKLIGAR+ NLA+    GK      SP
Sbjct: 148 GITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGARSMNLASQALKGKITTLDDSP 207

Query: 191 NDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAA 250
            DE+GHGTHTASTAAGTFV GAEALGNA G AVGMAPLAH+A+YKVC  + CS  DILA 
Sbjct: 208 IDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLAHLAIYKVCFGEDCSDVDILAG 267

Query: 251 LDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEA 310
           LDAA++DGVDVLS+SLG PS PFF D  A+G+FAAI+KGIFVSCSA NSGP   TL+NEA
Sbjct: 268 LDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKGIFVSCSAANSGPFNATLSNEA 327

Query: 311 PWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCV 370
           PWILTV ASTIDRKI A AKL +G+ F GESLFQP DF    LPLV+ G+   E    C 
Sbjct: 328 PWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFPQTLLPLVFPGEKN-ETVALCA 387

Query: 371 EGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPT 430
           EGSL+ ++V GK+VVCERGGGI RIAKG+ VKNGGGAAMIL+N + DGF+T  +AHVLP 
Sbjct: 388 EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMILLNAESDGFTTEVDAHVLPA 447

Query: 431 THLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILK 490
           +H+S+   LKIK YINS+  P A+I F+GT +G+    FSPA+ASFSSRGP  ASPGILK
Sbjct: 448 SHVSHTAALKIKAYINSTTYPTATILFKGTTIGD---DFSPAIASFSSRGPSLASPGILK 507

Query: 491 PDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPA 550
           PDITGPGV+ILAAWPFPL+N  NTNTKSTFN+ISGTSMSCPHLSGIAALIKS HP+WSPA
Sbjct: 508 PDITGPGVSILAAWPFPLDN--NTNTKSTFNIISGTSMSCPHLSGIAALIKSAHPDWSPA 567

Query: 551 AIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPY 610
           AIKS+IMT+A++ N +G PI+D+ L+PA+ FA+G+GHVNPSKA +PGLVYDIQPDDY+PY
Sbjct: 568 AIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAIDPGLVYDIQPDDYIPY 627

Query: 611 LCHL-YTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANS 670
           LC L YT+ QVS+I  + + C T S I EG+LNYPSF V LG   Q F+RTVT+VG    
Sbjct: 628 LCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLG-PVQTFSRTVTSVGSGRV 687

Query: 671 VYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWVSNKH 730
           VY  +++AP GVSV V PR L FS LN+K TYSVTF R   +    EF+EGYL WVS KH
Sbjct: 688 VYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSIEFAEGYLKWVSAKH 743

Query: 731 IVRSPISVK 733
           +VRSPISVK
Sbjct: 748 VVRSPISVK 743

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT17_ARATH6.0e-17746.39Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT12_ARATH3.9e-17646.55Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana GN=SBT1.2 PE=2 SV=1[more]
SBT14_ARATH1.1e-17046.11Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1[more]
SBT11_ARATH7.4e-16744.96Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana GN=SBTI1.1 PE=1 SV=1[more]
SBT18_ARATH1.8e-16545.09Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KMQ7_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157280 PE=4 SV=1[more]
A0A0A0KKE3_CUCSA1.2e-27767.22Uncharacterized protein OS=Cucumis sativus GN=Csa_5G157240 PE=4 SV=1[more]
B9RBY5_RICCO1.8e-27365.84Cucumisin, putative OS=Ricinus communis GN=RCOM_1682320 PE=4 SV=1[more]
B9GW37_POPTR3.2e-27065.93Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s11870g PE=4 SV=2[more]
A0A067JNI9_JATCU6.3e-26663.84Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20707 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G67360.13.4e-17846.39 Subtilase family protein[more]
AT1G04110.12.2e-17746.55 Subtilase family protein[more]
AT3G14067.16.2e-17246.11 Subtilase family protein[more]
AT1G01900.14.2e-16844.96 subtilase family protein[more]
AT2G05920.11.0e-16645.09 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|449459730|ref|XP_004147599.1|0.0e+00100.00PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus][more]
gi|659073650|ref|XP_008437177.1|0.0e+0093.73PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|659074655|ref|XP_008437722.1|6.2e-30771.76PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|449459724|ref|XP_004147596.1|1.8e-27767.22PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus][more]
gi|659073656|ref|XP_008437181.1|1.1e-27667.22PREDICTED: subtilisin-like protease [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023827Peptidase_S8_Asp-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G157280.1Csa5G157280.1mRNA


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 464..592
score: 2.2E-84coord: 71..315
score: 2.2
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 120..556
score: 8.3
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 96..354
score: 3.8E-84coord: 467..592
score: 3.8
IPR003137PA domainPFAMPF02225PAcoord: 348..435
score: 3.2
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 15..97
score: 5.3
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 186..199
score: 1.7E-20coord: 120..139
score: 1.7E-20coord: 518..534
score: 1.7
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 10..733
score:
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 125..136
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 342..444
score: 7.1
NoneNo IPR availablePANTHERPTHR10795:SF370SUBFAMILY NOT NAMEDcoord: 10..733
score:
NoneNo IPR availableunknownSSF52025PA domaincoord: 348..442
score: 3.6

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa5G157280Cucurbita pepo (Zucchini)cpecuB046
Csa5G157280Cucurbita pepo (Zucchini)cpecuB174
Csa5G157280Cucurbita pepo (Zucchini)cpecuB733
Csa5G157280Cucurbita pepo (Zucchini)cpecuB872
Csa5G157280Bottle gourd (USVL1VR-Ls)culsiB322
Csa5G157280Bottle gourd (USVL1VR-Ls)culsiB376
Csa5G157280Bottle gourd (USVL1VR-Ls)culsiB386
Csa5G157280Cucumber (Gy14) v2cgybcuB128
Csa5G157280Cucumber (Gy14) v2cgybcuB129
Csa5G157280Cucumber (Gy14) v2cgybcuB224
Csa5G157280Melon (DHL92) v3.6.1cumedB336
Csa5G157280Melon (DHL92) v3.6.1cumedB389
Csa5G157280Melon (DHL92) v3.6.1cumedB390
Csa5G157280Melon (DHL92) v3.6.1cumedB391
Csa5G157280Silver-seed gourdcarcuB0519
Csa5G157280Silver-seed gourdcarcuB0882
Csa5G157280Silver-seed gourdcarcuB0954
Csa5G157280Watermelon (97103) v2cuwmbB336
Csa5G157280Watermelon (97103) v2cuwmbB356
Csa5G157280Wax gourdcuwgoB438
Csa5G157280Cucumber (Chinese Long) v2cucuB096
Csa5G157280Cucumber (Chinese Long) v2cucuB100
Csa5G157280Cucumber (Chinese Long) v2cucuB102
Csa5G157280Cucumber (Gy14) v1cgycuB368
Csa5G157280Cucumber (Gy14) v1cgycuB434
Csa5G157280Cucumber (Gy14) v1cgycuB461
Csa5G157280Cucurbita maxima (Rimu)cmacuB298
Csa5G157280Cucurbita maxima (Rimu)cmacuB420
Csa5G157280Cucurbita maxima (Rimu)cmacuB608
Csa5G157280Cucurbita maxima (Rimu)cmacuB614
Csa5G157280Cucurbita maxima (Rimu)cmacuB838
Csa5G157280Cucurbita moschata (Rifu)cmocuB286
Csa5G157280Cucurbita moschata (Rifu)cmocuB411
Csa5G157280Cucurbita moschata (Rifu)cmocuB598
Csa5G157280Cucurbita moschata (Rifu)cmocuB606
Csa5G157280Cucurbita moschata (Rifu)cmocuB826
Csa5G157280Melon (DHL92) v3.5.1cumeB357
Csa5G157280Melon (DHL92) v3.5.1cumeB401
Csa5G157280Melon (DHL92) v3.5.1cumeB405
Csa5G157280Watermelon (Charleston Gray)cuwcgB351
Csa5G157280Watermelon (Charleston Gray)cuwcgB364
Csa5G157280Watermelon (97103) v1cuwmB403
Csa5G157280Watermelon (97103) v1cuwmB408