Csa1G070080.1 (mRNA) Cucumber (Chinese Long) v2

NameCsa1G070080.1
TypemRNA
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionUnknown protein; contains IPR008930 (Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid)
LocationChr1 : 7042036 .. 7042800 (-)
Sequence length654
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATCTTCAATCCTTTCTTGCTTCTCCCTGTCCAAAAATATTCATTTATCAAACAAAAAATCCACACAAAGTTCAGAATAACAACGCATGGCTTCAATTGAATGACCCTCTTGAAAAATCTTTTGCCAGAAAAGGGTGCATTCATGCCTTGAATTCTTCTTCATCATCCAATAAGTGCATCATCTCCAATTTGAAAATTGTATATTATCTTATTATTTTGGTCCATTGATTTTGTCTCCTTATGATTTCTCCTTTCATTTATTTATTTATTATTTTTTATGATGAGTCTTATAAATGATGATGTGTATAGGAGGAGTTTGGGGTTGAAACTCCAGCGAGAAAAGTGGAGATTTTAAAGGACATACTAACAGAAACAAGAGATGATCTGTGTTGGGAACGTTTGGAAATAATTGATGCAGCCCAATGGCTTGGCATTGACAACCAGTTCGAAGAAGAGATTGAAGATGTTCTTAAGAGGCAATATGATTTGATAAATGCATATCGTTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTCCTTTTTCGACTTTTGAGACAACAACGATACCTTATATCTCAAGGTTCATATTCCACACTCTTTACCAACGAATTAAGCTTGAAGGTCTATAAGAAACATGGTTGGAATCCCATTGGTTCCTTGAGAAAAACGGTATTTTTTGTTAGATCCAAATTTAGCATTTTGGAAACCAAACGGATTAACAAATGCTATATGACAGGGAAGTACTTACAAAAACATTAA

mRNA sequence

ATGTATCTTCAATCCTTTCTTGCTTCTCCCTGTCCAAAAATATTCATTTATCAAACAAAAAATCCACACAAAGTTCAGAATAACAACGCATGGCTTCAATTGAATGACCCTCTTGAAAAATCTTTTGCCAGAAAAGGGTGCATTCATGCCTTGAATTCTTCTTCATCATCCAATAAGTGCATCATCTCCAATTTGAAAATTGAGGAGTTTGGGGTTGAAACTCCAGCGAGAAAAGTGGAGATTTTAAAGGACATACTAACAGAAACAAGAGATGATCTGTGTTGGGAACGTTTGGAAATAATTGATGCAGCCCAATGGCTTGGCATTGACAACCAGTTCGAAGAAGAGATTGAAGATGTTCTTAAGAGGCAATATGATTTGATAAATGCATATCGTTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTCCTTTTTCGACTTTTGAGACAACAACGATACCTTATATCTCAAGGTTCATATTCCACACTCTTTACCAACGAATTAAGCTTGAAGGTCTATAAGAAACATGGTTGGAATCCCATTGGTTCCTTGAGAAAAACGGTATTTTTTGTTAGATCCAAATTTAGCATTTTGGAAACCAAACGGATTAACAAATGCTATATGACAGGGAAGTACTTACAAAAACATTAA

Coding sequence (CDS)

ATGTATCTTCAATCCTTTCTTGCTTCTCCCTGTCCAAAAATATTCATTTATCAAACAAAAAATCCACACAAAGTTCAGAATAACAACGCATGGCTTCAATTGAATGACCCTCTTGAAAAATCTTTTGCCAGAAAAGGGTGCATTCATGCCTTGAATTCTTCTTCATCATCCAATAAGTGCATCATCTCCAATTTGAAAATTGAGGAGTTTGGGGTTGAAACTCCAGCGAGAAAAGTGGAGATTTTAAAGGACATACTAACAGAAACAAGAGATGATCTGTGTTGGGAACGTTTGGAAATAATTGATGCAGCCCAATGGCTTGGCATTGACAACCAGTTCGAAGAAGAGATTGAAGATGTTCTTAAGAGGCAATATGATTTGATAAATGCATATCGTTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTCCTTTTTCGACTTTTGAGACAACAACGATACCTTATATCTCAAGGTTCATATTCCACACTCTTTACCAACGAATTAAGCTTGAAGGTCTATAAGAAACATGGTTGGAATCCCATTGGTTCCTTGAGAAAAACGGTATTTTTTGTTAGATCCAAATTTAGCATTTTGGAAACCAAACGGATTAACAAATGCTATATGACAGGGAAGTACTTACAAAAACATTAA

Protein sequence

MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKCIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWNPIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH*
BLAST of Csa1G070080.1 vs. Swiss-Prot
Match: TPS13_RICCO (Probable terpene synthase 13 OS=Ricinus communis GN=TPS13 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.5e-09
Identity = 42/111 (37.84%), Postives = 60/111 (54.05%), Query Frame = 1

Query: 56  SSNKCIISNLKIE-----EFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGID 115
           S N+ ++S L  +     +  V    R+    K IL +  +    E L +IDA Q LGID
Sbjct: 43  SINQTLVSPLSAQCIHLTDEDVRIKERRTRAFKHILRKEGEG-SHEVLAMIDAIQRLGID 102

Query: 116 NQFEEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSY 162
           + F++EI+++L+RQY + + Y    D DLH  AL FRLLRQ  Y +S G +
Sbjct: 103 HHFQDEIDEILQRQYTIPSYY---NDNDLHGLALRFRLLRQGGYNVSAGVF 149


HSP 2 Score: 42.0 bits (97), Expect = 1.0e-02
Identity = 16/23 (69.57%), Postives = 18/23 (78.26%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLRKT 189
           TNE+  KVYK+HGWNP+ SLR+T
Sbjct: 371 TNEIGYKVYKQHGWNPVHSLRET 393

BLAST of Csa1G070080.1 vs. Swiss-Prot
Match: NES1_FRAAN ((3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 9.3e-09
Identity = 33/87 (37.93%), Postives = 52/87 (59.77%), Query Frame = 1

Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDG 135
           ++K+E+LK +L    +    E L +IDA Q LGID  F+ EI+++L +Q  +++A     
Sbjct: 19  SQKLELLKTVLRNVAELDALEGLNMIDAVQRLGIDYNFQREIDEILHKQMSIVSARD--- 78

Query: 136 DMDLHKAALLFRLLRQQRYLISQGSYS 163
             DLH+ AL FRLLRQ  Y + +  ++
Sbjct: 79  --DLHEVALRFRLLRQHGYFVPEDVFN 100


HSP 2 Score: 37.4 bits (85), Expect = 2.5e-01
Identity = 15/21 (71.43%), Postives = 15/21 (71.43%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLR 187
           TNE S KVY KHGWNP+ SL+
Sbjct: 318 TNEFSSKVYLKHGWNPLQSLK 338

BLAST of Csa1G070080.1 vs. Swiss-Prot
Match: NES2_FRAAN ((3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-07
Identity = 31/86 (36.05%), Postives = 49/86 (56.98%), Query Frame = 1

Query: 77  RKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGD 136
           +K+E+ +++L    +    E L +IDA Q LGID  F+ EI+++L +Q   ++A      
Sbjct: 79  QKLELFRNVLRNVAELDALEGLNMIDAVQRLGIDFHFQREIDEILHKQMSNVSA-----S 138

Query: 137 MDLHKAALLFRLLRQQRYLISQGSYS 163
            DLH+ AL FRLLRQ  Y + +  ++
Sbjct: 139 DDLHEVALRFRLLRQHGYFVPEDVFN 159


HSP 2 Score: 39.7 bits (91), Expect = 4.9e-02
Identity = 15/21 (71.43%), Postives = 17/21 (80.95%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLR 187
           TNE+S KVY+KHGWNP+ SL+
Sbjct: 377 TNEISCKVYQKHGWNPLQSLK 397

BLAST of Csa1G070080.1 vs. Swiss-Prot
Match: NES1_FRAVE ((3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.3e-07
Identity = 30/87 (34.48%), Postives = 51/87 (58.62%), Query Frame = 1

Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDG 135
           ++K+E+ +++L    +    E L +IDA Q LGID  F+ EI+++L +Q  +++A     
Sbjct: 80  SQKLELFRNVLRNAAELDALEGLNMIDAVQRLGIDYHFQREIDEILHKQMGIVSACD--- 139

Query: 136 DMDLHKAALLFRLLRQQRYLISQGSYS 163
             DL++ AL FRLLRQ  Y + +  ++
Sbjct: 140 --DLYEVALRFRLLRQHGYFVPEDVFN 161


HSP 2 Score: 38.9 bits (89), Expect = 8.4e-02
Identity = 15/21 (71.43%), Postives = 16/21 (76.19%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLR 187
           TNE S KVY+KHGWNP+ SL+
Sbjct: 379 TNEFSCKVYQKHGWNPLRSLK 399

BLAST of Csa1G070080.1 vs. Swiss-Prot
Match: MYRS_QUEIL (Myrcene synthase, chloroplastic OS=Quercus ilex PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.1e-07
Identity = 49/150 (32.67%), Postives = 75/150 (50.00%), Query Frame = 1

Query: 58  NKCIISNLKIEEFGVETPARKVEILKD----ILTETRDDLCWERLEIIDAAQWLGIDNQF 117
           N   I +L+IE  G ET  R++ +LK+    +L +  + L  E+LE+I+  Q LG+   F
Sbjct: 68  NHDYIESLRIEYVG-ETCTRQINVLKEQVRMMLHKVVNPL--EQLELIEILQRLGLSYHF 127

Query: 118 EEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKV 177
           EEEI+ +L   Y+  +        +L+  AL FRLLRQ  Y +SQ  +++      S K 
Sbjct: 128 EEEIKRILDGVYNNDHGGDTWKAENLYATALKFRLLRQHGYSVSQEVFNSFKDERGSFKA 187

Query: 178 YKKHGWNPIGSLRKTVFFVRSKFSILETKR 204
                   + SL +  FF+    +ILE  R
Sbjct: 188 CLCEDTKGMLSLYEASFFLIEGENILEEAR 214

BLAST of Csa1G070080.1 vs. TrEMBL
Match: A0A0A0LX82_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G070080 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 9.7e-122
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 1

Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWN 180
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWN
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWN 180

Query: 181 PIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH 218
           PIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH
Sbjct: 181 PIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH 217

BLAST of Csa1G070080.1 vs. TrEMBL
Match: A0A0A0LUC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G068570 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-16
Identity = 68/155 (43.87%), Postives = 85/155 (54.84%), Query Frame = 1

Query: 26  QNNNAWLQLNDPLEKSFARKGCIHALNSS------SSSNKCIISNLKIEEFGVE--TPAR 85
           QNNN    +N+PL          H+L S+      S     I+SN      G+E    A 
Sbjct: 20  QNNNFNPIINNPLLNKQIIYLPHHSLLSTPLKPFDSKIQPSILSN------GIEYGEAAI 79

Query: 86  KVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGDM 145
           K  ILKD+L E  D   WE L +IDA Q LGID  F+EEIE VL+RQY L NA +++ D 
Sbjct: 80  KGRILKDVLREIGDP--WECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAIQYNPDT 139

Query: 146 DLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLK 173
           DLHKAALLFRL RQQ YL+S   + +    +   K
Sbjct: 140 DLHKAALLFRLFRQQGYLVSADVFKSFMDKKGKFK 166

BLAST of Csa1G070080.1 vs. TrEMBL
Match: A0A0A0LUC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G068570 PE=3 SV=1)

HSP 1 Score: 34.7 bits (78), Expect = 1.8e+02
Identity = 13/22 (59.09%), Postives = 16/22 (72.73%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLRK 188
           TNE+   +Y+KHGWNPI  L K
Sbjct: 373 TNEICYHIYQKHGWNPIHFLHK 394


HSP 2 Score: 72.8 bits (177), Expect = 5.9e-10
Identity = 56/169 (33.14%), Postives = 84/169 (49.70%), Query Frame = 1

Query: 6   FLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKCIISNL 65
           F+     K+      N + +  NN    +ND +  S   K   +    SS+ N     ++
Sbjct: 17  FIPKHISKVGNRNLNNINSLLPNNKKGSINDNIGVSARLKRFTYPSEHSSNFN----DDI 76

Query: 66  KIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQY 125
            I+       A+K+E++K IL +  DD  +E L +ID  Q LGID  F++EIE +L+RQY
Sbjct: 77  HIKH------AKKLEVIKHILIKLGDDDSFEGLAMIDVVQRLGIDYYFQDEIELILRRQY 136

Query: 126 DLINAYRFDGDM--DLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLK 173
            +   +  DGD   DL + AL FRLLRQQ Y +S   ++     E   K
Sbjct: 137 SI---FFTDGDRYNDLQEVALRFRLLRQQGYYVSADVFNRFRNKEGEFK 172

BLAST of Csa1G070080.1 vs. TrEMBL
Match: A0A0A0LX78_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G066550 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-09
Identity = 43/101 (42.57%), Postives = 61/101 (60.40%), Query Frame = 1

Query: 78  KVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYR-FDGD 137
           +++ILK +L+ET  +   E L +ID  Q LGID+ F+EEIE +LK QY ++  +  FD  
Sbjct: 94  EMKILKHLLSETAKNGSLEVLNMIDGIQRLGIDHCFKEEIEAILKEQYTIMATHHYFDTK 153

Query: 138 MDLHKAALLFRLLRQQRYLISQGSYS--TLFTNELSLKVYK 176
             LH+ AL FRLLRQ  Y I    +   T    EL+ KV++
Sbjct: 154 CSLHEVALRFRLLRQHGYFIPSDVFESFTAKNGELNKKVFE 194

BLAST of Csa1G070080.1 vs. TrEMBL
Match: A0A0A0LX78_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G066550 PE=3 SV=1)

HSP 1 Score: 34.3 bits (77), Expect = 2.3e+02
Identity = 31/92 (33.70%), Postives = 43/92 (46.74%), Query Frame = 1

Query: 110 DNQFEEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQ--QRYLIS---------Q 169
           D +F EE  ++ K    +   Y  D   DL+ +    RL  Q  QR+ +          +
Sbjct: 330 DPKFSEERVELTK---SISFIYLLDDIYDLYGSIEELRLFTQAIQRWNLEAINSLPHPMK 389

Query: 170 GSYSTLF--TNELSLKVYKKHGWNPIGSLRKT 189
             Y  L+  TNELS K   KHGWNPI  L+K+
Sbjct: 390 FCYIKLYETTNELSHKFSLKHGWNPIHCLQKS 418


HSP 2 Score: 69.7 bits (169), Expect = 5.0e-09
Identity = 41/113 (36.28%), Postives = 68/113 (60.18%), Query Frame = 1

Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLI---NAYR 135
           ARK+++ + +L++  ++   E L +IDA Q LGID+ F+EEI+ +L++Q+ +I   +A+ 
Sbjct: 71  ARKLDVFRHVLSKLGEEAV-EGLNMIDAVQRLGIDHHFQEEIDQILQKQHLIITSGSAHG 130

Query: 136 FDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWNPIGSL 186
            D   DLH+ A+ FRLLRQQ Y +    ++    +E S K     G   + SL
Sbjct: 131 ADHHSDLHEVAVRFRLLRQQGYFVPDDVFNKFKESEGSFKKMLSEGIQGLMSL 182

BLAST of Csa1G070080.1 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 53.5 bits (127), Expect = 1.9e-07
Identity = 40/133 (30.08%), Postives = 66/133 (49.62%), Query Frame = 1

Query: 58  NKCIISNLKIEEFGVETPARKVEILKDILTETRDD---LCWERLEIIDAAQWLGIDNQFE 117
           N C + +++  ++  ET     ++LK  +    DD      E+LE+ID  Q LG+   FE
Sbjct: 58  NHCYLLSIE-NKYASETEVITRDVLKKKVKSMLDDEKKSRLEQLELIDDLQKLGVSYHFE 117

Query: 118 EEIEDVLKRQYDLI--NAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLK 177
            EI D L   +  +  N ++ D + DLH  +L FRLLRQ  + +S+  +  +  +++   
Sbjct: 118 IEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEFRLLRQHGFDVSENIFDVII-DQIESN 177

Query: 178 VYKKHGWNPIGSL 186
            +K +  N I SL
Sbjct: 178 TFKTNNINGIISL 188

BLAST of Csa1G070080.1 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 53.5 bits (127), Expect = 1.9e-07
Identity = 40/133 (30.08%), Postives = 66/133 (49.62%), Query Frame = 1

Query: 58  NKCIISNLKIEEFGVETPARKVEILKDILTETRDD---LCWERLEIIDAAQWLGIDNQFE 117
           N C + +++  ++  ET     ++LK  +    DD      E+LE+ID  Q LG+   FE
Sbjct: 58  NHCYLLSIE-NKYASETEVITRDVLKKKVKSMLDDEKKSRLEQLELIDDLQKLGVSYHFE 117

Query: 118 EEIEDVLKRQYDLI--NAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLK 177
            EI D L   +  +  N ++ D + DLH  +L FRLLRQ  + +S+  +  +  +++   
Sbjct: 118 IEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEFRLLRQHGFDVSENIFDVII-DQIESN 177

Query: 178 VYKKHGWNPIGSL 186
            +K +  N I SL
Sbjct: 178 TFKTNNINGIISL 188

BLAST of Csa1G070080.1 vs. TAIR10
Match: AT3G25810.1 (AT3G25810.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 52.4 bits (124), Expect = 4.2e-07
Identity = 35/105 (33.33%), Postives = 53/105 (50.48%), Query Frame = 1

Query: 62  ISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVL 121
           I N  + E  V T     + +K +L E       E+LE+ID  Q LG+   FE+EI ++L
Sbjct: 65  IENKYVNEKEVITRHVLKKKVKKMLEEVETKSRLEKLELIDDLQKLGVSYHFEQEINNIL 124

Query: 122 KRQY--DLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTL 165
              +  +  N ++ D + DLH  AL FRLLRQ  + +S+  +  +
Sbjct: 125 TNFHLENGKNIWKCDKEEDLHATALEFRLLRQHGFGVSEDIFDVI 169

BLAST of Csa1G070080.1 vs. TAIR10
Match: AT1G61680.1 (AT1G61680.1 terpene synthase 14)

HSP 1 Score: 51.6 bits (122), Expect = 7.1e-07
Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 1

Query: 43  ARKGCIHALNSSSSSNKCIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIID 102
           A++  +  +++S S+      N    E+  E   +K+   K+IL+    D+  E LE+ID
Sbjct: 43  AKRSILRNVHASVSNPSKQFHNKTSLEYLHELNIKKI---KNILS-ANVDVPSENLEMID 102

Query: 103 AAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYS 162
             Q LGID  F +EIE  L   Y      +F+G  DLH+ AL FRLLRQ+ + + +    
Sbjct: 103 VIQSLGIDLHFRQEIEQTLHMIYK--EGLQFNG--DLHEIALRFRLLRQEGHYVQE---- 160

Query: 163 TLFTNELSLK 173
            +F N L  K
Sbjct: 163 IIFKNILDKK 160


HSP 2 Score: 40.4 bits (93), Expect = 1.6e-03
Identity = 20/54 (37.04%), Postives = 32/54 (59.26%), Query Frame = 1

Query: 164 LFTNELSLKVYKKHGWNPIGSLRKTVFFVRSKFSILETKRINKCYM--TGKYLQ 216
           + T E+S+K+YK HGWNP  +LR++   +   F ++E K  N  Y+  T +Y++
Sbjct: 374 MITTEISMKIYKSHGWNPTYALRQSWASLCKAF-LVEAKWFNSGYLPTTEEYMK 426

BLAST of Csa1G070080.1 vs. TAIR10
Match: AT4G16730.1 (AT4G16730.1 terpene synthase 02)

HSP 1 Score: 50.1 bits (118), Expect = 2.1e-06
Identity = 39/134 (29.10%), Postives = 68/134 (50.75%), Query Frame = 1

Query: 72  VETPARKVEILKDILTETRDDL--CWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLIN 131
           VET A K  + K+ + +T +++    E+LE+ID+ Q LGI   ++ EI D+L++ +D   
Sbjct: 18  VET-AEKAILFKEEVRKTLNEIEGSIEQLEMIDSLQRLGISYHYKHEIHDILRKIHDQHG 77

Query: 132 AYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWNPIGSLRKTV 191
               +   DLH  +L F LLRQ  + +SQ ++    +     +         + SL +  
Sbjct: 78  EIERE-TQDLHATSLEFILLRQHGFDVSQDAFDVFISETGEFRKTLHSDIKGLLSLYEAS 137

Query: 192 FF-VRSKFSILETK 203
           +F + S+F + ET+
Sbjct: 138 YFSMDSEFKLKETR 149

BLAST of Csa1G070080.1 vs. NCBI nr
Match: gi|778658558|ref|XP_011652894.1| (PREDICTED: myrcene synthase, chloroplastic-like isoform X1 [Cucumis sativus])

HSP 1 Score: 444.1 bits (1141), Expect = 1.4e-121
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 1

Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWN 180
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWN
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGWN 180

Query: 181 PIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH 218
           PIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH
Sbjct: 181 PIGSLRKTVFFVRSKFSILETKRINKCYMTGKYLQKH 217

BLAST of Csa1G070080.1 vs. NCBI nr
Match: gi|778658562|ref|XP_011652895.1| (PREDICTED: probable terpene synthase 13 isoform X2 [Cucumis sativus])

HSP 1 Score: 325.5 bits (833), Expect = 7.2e-86
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 1

Query: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60
           MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120
           IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 160
           LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQG 159

BLAST of Csa1G070080.1 vs. NCBI nr
Match: gi|659068831|ref|XP_008446523.1| (PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo])

HSP 1 Score: 262.3 bits (669), Expect = 7.5e-67
Identity = 140/186 (75.27%), Postives = 151/186 (81.18%), Query Frame = 1

Query: 1   MYLQSFLA-SPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNK 60
           +YLQSFLA  P PKIFI QTK PHK  NNNAWLQLN+PLEKSFA KG IHALNSSSSSNK
Sbjct: 5   IYLQSFLAFPPRPKIFISQTKIPHKAHNNNAWLQLNNPLEKSFAEKGFIHALNSSSSSNK 64

Query: 61  CIISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIED 120
            IIS+LKIEE GVETPARKV+ILK++LTETRDDLC E LEIIDAAQ LGIDNQFEEEIED
Sbjct: 65  IIISDLKIEELGVETPARKVKILKELLTETRDDLCLECLEIIDAAQRLGIDNQFEEEIED 124

Query: 121 VLKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLKVYKKHGW 180
           VLKRQY LINAY FDGD+DLHKAALLFRLLRQQRYL+SQ  +      +   K   K   
Sbjct: 125 VLKRQYALINAYHFDGDVDLHKAALLFRLLRQQRYLVSQDVFKVFLNEKGKFKEKLKADL 184

Query: 181 NPIGSL 186
           N + SL
Sbjct: 185 NGMTSL 190

BLAST of Csa1G070080.1 vs. NCBI nr
Match: gi|659068831|ref|XP_008446523.1| (PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo])

HSP 1 Score: 52.4 bits (124), Expect = 1.2e-03
Identity = 22/23 (95.65%), Postives = 23/23 (100.00%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLRKT 189
           TNELSLKVYKKHGWNP+GSLRKT
Sbjct: 393 TNELSLKVYKKHGWNPVGSLRKT 415


HSP 2 Score: 95.1 bits (235), Expect = 1.6e-16
Identity = 68/155 (43.87%), Postives = 85/155 (54.84%), Query Frame = 1

Query: 26  QNNNAWLQLNDPLEKSFARKGCIHALNSS------SSSNKCIISNLKIEEFGVE--TPAR 85
           QNNN    +N+PL          H+L S+      S     I+SN      G+E    A 
Sbjct: 20  QNNNFNPIINNPLLNKQIIYLPHHSLLSTPLKPFDSKIQPSILSN------GIEYGEAAI 79

Query: 86  KVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDGDM 145
           K  ILKD+L E  D   WE L +IDA Q LGID  F+EEIE VL+RQY L NA +++ D 
Sbjct: 80  KGRILKDVLREIGDP--WECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAIQYNPDT 139

Query: 146 DLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLK 173
           DLHKAALLFRL RQQ YL+S   + +    +   K
Sbjct: 140 DLHKAALLFRLFRQQGYLVSADVFKSFMDKKGKFK 166

BLAST of Csa1G070080.1 vs. NCBI nr
Match: gi|700209493|gb|KGN64589.1| (hypothetical protein Csa_1G068570 [Cucumis sativus])

HSP 1 Score: 34.7 bits (78), Expect = 2.5e+02
Identity = 13/22 (59.09%), Postives = 16/22 (72.73%), Query Frame = 1

Query: 166 TNELSLKVYKKHGWNPIGSLRK 188
           TNE+   +Y+KHGWNPI  L K
Sbjct: 373 TNEICYHIYQKHGWNPIHFLHK 394


HSP 2 Score: 93.2 bits (230), Expect = 6.0e-16
Identity = 52/97 (53.61%), Postives = 63/97 (64.95%), Query Frame = 1

Query: 76  ARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDVLKRQYDLINAYRFDG 135
           A K  ILKD+L E  D   WE L +IDA Q LGID  F+EEIE VL+RQY L NA +++ 
Sbjct: 60  AIKGRILKDVLREIGDP--WECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAIQYNP 119

Query: 136 DMDLHKAALLFRLLRQQRYLISQGSYSTLFTNELSLK 173
           D DLHKAALLFRL RQQ YL+S   + +    +   K
Sbjct: 120 DTDLHKAALLFRLFRQQGYLVSADVFKSFMDKKGKFK 154

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TPS13_RICCO5.5e-0937.84Probable terpene synthase 13 OS=Ricinus communis GN=TPS13 PE=3 SV=1[more]
NES1_FRAAN9.3e-0937.93(3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa PE=1 SV=1[more]
NES2_FRAAN1.8e-0736.05(3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa P... [more]
NES1_FRAVE2.3e-0734.48(3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca PE=1 SV=1[more]
MYRS_QUEIL5.1e-0732.67Myrcene synthase, chloroplastic OS=Quercus ilex PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LX82_CUCSA9.7e-122100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G070080 PE=3 SV=1[more]
A0A0A0LUC8_CUCSA1.1e-1643.87Uncharacterized protein OS=Cucumis sativus GN=Csa_1G068570 PE=3 SV=1[more]
A0A0A0LUC8_CUCSA1.8e+0259.09Uncharacterized protein OS=Cucumis sativus GN=Csa_1G068570 PE=3 SV=1[more]
A0A0A0LX78_CUCSA2.9e-0942.57Uncharacterized protein OS=Cucumis sativus GN=Csa_1G066550 PE=3 SV=1[more]
A0A0A0LX78_CUCSA2.3e+0233.70Uncharacterized protein OS=Cucumis sativus GN=Csa_1G066550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G25820.11.9e-0730.08 terpene synthase-like sequence-1,8-cineole[more]
AT3G25830.11.9e-0730.08 terpene synthase-like sequence-1,8-cineole[more]
AT3G25810.14.2e-0733.33 Terpenoid cyclases/Protein prenyltransferases superfamily protein[more]
AT1G61680.17.1e-0733.85 terpene synthase 14[more]
AT4G16730.12.1e-0629.10 terpene synthase 02[more]
Match NameE-valueIdentityDescription
gi|778658558|ref|XP_011652894.1|1.4e-121100.00PREDICTED: myrcene synthase, chloroplastic-like isoform X1 [Cucumis sativus][more]
gi|778658562|ref|XP_011652895.1|7.2e-86100.00PREDICTED: probable terpene synthase 13 isoform X2 [Cucumis sativus][more]
gi|659068831|ref|XP_008446523.1|7.5e-6775.27PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo][more]
gi|659068831|ref|XP_008446523.1|1.2e-0395.65PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo][more]
gi|700209493|gb|KGN64589.1|2.5e+0259.09hypothetical protein Csa_1G068570 [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR001906Terpene_synth_N
IPR008930Terpenoid_cyclase/PrenylTrfase
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0010333terpene synthase activity
GO:0016829lyase activity
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csa1G070080Csa1G070080gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csa1G070080.1Csa1G070080.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csa1G070080.1.cds2Csa1G070080.1.cds2CDS
Csa1G070080.1.cds1Csa1G070080.1.cds1CDS


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainGENE3DG3DSA:1.50.30.10coord: 82..161
score: 2.5
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 79..166
score: 1.5
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidunknownSSF48239Terpenoid cyclases/Protein prenyltransferasescoord: 79..175
score: 4.94
NoneNo IPR availablePANTHERPTHR31225FAMILY NOT NAMEDcoord: 80..159
score: 1.8