Csa1G050300.2 (mRNA) Cucumber (Chinese Long) v2

BLAST of Csa1G050300.2 vs. Swiss-Prot
Match: 4CLL9_ARATH (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 522.7 bits (1345), Expect = 4.1e-147
Identity = 277/461 (60.09%), Postives = 342/461 (74.19%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPA-------LI 65
           +D  SGF  +T IY+SLRP LSLPP+ QPL+    ALS+L  S PP            L+
Sbjct: 11  IDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLV 70

Query: 66  DSDSSVSVSYALFLRQIRNLTSNLKALY-SFSNGQVAFILSPTSLQIPVLYFVLLSLGVV 125
           +S S  +++Y   LR++R+L  +L+  + S ++  VAFILSP+SL IPVLY  L+S+GVV
Sbjct: 71  NSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV 130

Query: 126 VSPANPIGSESEIAHQVSLCIPAIAFATSSTASKIPR--LPLGTVLIDSPEFLSLMNESN 185
           VSPANPIGSESE++HQV +  P IAFATS T  K+    LPLGTVL+DS EFLS +N S+
Sbjct: 131 VSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSD 190

Query: 186 PSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGE 245
            S  VN   F +++NQ+D AAIL+SSGTTGRVKGVL++HRNLI +     V  +  +   
Sbjct: 191 -SSSVNP--FQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTA---VSHQRTLQDP 250

Query: 246 IEPHPVALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPP 305
           +    V L  LPLFHVFGF M+ R+IS G TLVL+ +F+ E M +AVEKY++T +PVSPP
Sbjct: 251 VNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPP 310

Query: 306 LIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAA 365
           LIVA+ KSEL  KYDL SL+ L CGGAPLGK++ ++F  KFP+V+I+QGYGLTESSG AA
Sbjct: 311 LIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAA 370

Query: 366 RTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERAT 425
            T GPEE    GSVGR+SENMEAKIVDPS+GE+LPPG  GELW+RGP IMKGYVG+E+A+
Sbjct: 371 STFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKAS 430

Query: 426 AETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           AET+  EGWLKTGDLCYFDS+  LYIVDRLKELIKYKAYQV
Sbjct: 431 AETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQV 465

BLAST of Csa1G050300.2 vs. Swiss-Prot
Match: 4CLL6_ORYSJ (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 428.3 bits (1100), Expect = 1.1e-118
Identity = 233/466 (50.00%), Postives = 301/466 (64.59%), Query Frame = 1

Query: 8   SRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPP---PPNTPALIDSDSSV 67
           SRSGFC+ T+ ++SLR    LPP   PLTV  +A S+L S+PP       PAL+D+ + +
Sbjct: 40  SRSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGI 99

Query: 68  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 127
           +VSY  F+ ++R L   L        G VA ++SP+ L + VLYF L+S+GVVVSPANP 
Sbjct: 100 AVSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPA 159

Query: 128 GSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDGI 187
            +  E AHQV L  PAIAF     A+++PR  +  V+I S  F  L + S          
Sbjct: 160 STADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVFDRLASASAAGGWAAPPA 219

Query: 188 FDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVD------GEIEP 247
             +K  Q  +AA+LYSSGTTGRVK V I+HRNLI  I+    + +T+        G+ +P
Sbjct: 220 VAMK--QPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKP 279

Query: 248 HP--------VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYI 307
            P        V L  LPLFHV GF +L R+IS G T V+MR+FD     RAVE+YR+T +
Sbjct: 280 PPPSPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKL 339

Query: 308 PVSPPLIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES 367
             +PP++VA+ KS+ A + DLSSL  +  GGAPLG+EV  +F   FP+V+I+Q YGLTES
Sbjct: 340 SAAPPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTES 399

Query: 368 SGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVG 427
           +G  A   GPEE +  GSVGRL+  ++AKIVD ++GE L PG +GELWIRGP +MKGYVG
Sbjct: 400 TGPVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVG 459

Query: 428 DERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           D  ATA T+ P+GWLKTGDLCYF+ DG LY+VDRLKELIKYK YQV
Sbjct: 460 DPEATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQV 502

BLAST of Csa1G050300.2 vs. Swiss-Prot
Match: 4CLL5_ORYSJ (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.4e-115
Identity = 232/459 (50.54%), Postives = 299/459 (65.14%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVS 65
           +DSRSGFC+ T+I++S R P  LPP S P+T   +A S+L SS  P   PAL+D+ + ++
Sbjct: 15  IDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLP-GRPALVDAATGIA 74

Query: 66  VSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPIG 125
           +SY  FL  +R+L   L        G VA +++P+ L++PVL F L+S+G VVSPANP+ 
Sbjct: 75  ISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFALMSIGAVVSPANPVS 134

Query: 126 SESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDGI- 185
           +  E AHQV+L  P +AFA    A+K+P   +  V+I S E+  L      SDG      
Sbjct: 135 TPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEYGRLA----ASDGRRAAAP 194

Query: 186 FDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEI-----EPH 245
             + + Q+D+AA+LYSSGTTGRVK V I+HRNLI A+  L   ++  V  E      EP 
Sbjct: 195 AAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLI-ALMSLHADNREKVAREAAEAGEEPP 254

Query: 246 PVALCLLP--LFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLI 305
           P A+ LLP  LFHVFGF M+ RS+S G T VLM +FDF   LRA+E+YR+T +P +PP++
Sbjct: 255 PPAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVTLLPAAPPVL 314

Query: 306 VAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAART 365
           VAM K E A + DLSSL ++  GGAPLG+EV ++F   FPNVE++QGYGLTESSGA A T
Sbjct: 315 VAMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLTESSGAVAAT 374

Query: 366 VGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAE 425
           VGPEE    GSVG+L  +++AKIVDPS+                     GYVGD+ ATA 
Sbjct: 375 VGPEESKAYGSVGKLGSHLQAKIVDPST---------------------GYVGDDEATAA 434

Query: 426 TLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           T+  EGWLKTGDLCYF+ DG LYIVDRLKELIKYK YQV
Sbjct: 435 TVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQV 445

BLAST of Csa1G050300.2 vs. Swiss-Prot
Match: 4CLL7_ORYSJ (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 6.5e-100
Identity = 196/459 (42.70%), Postives = 279/459 (60.78%), Query Frame = 1

Query: 8   SRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVL------RSSPPPPNTPALIDSD 67
           +R+G+C+ TK + SLRPP+ LPP   PL+    A S+L       SS   P  PAL+D+ 
Sbjct: 15  TRTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSSSSSSLLPANPALVDAA 74

Query: 68  SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 127
           +  +VS+  FL ++R L   L++      G VAF+L+P  L +PVLYF LLS+G VVSPA
Sbjct: 75  TGEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPA 134

Query: 128 NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 187
           NP  + +E++  VSL   ++AFA SSTA+K+P      VL+DSP F SL+ +   + G  
Sbjct: 135 NPALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQG-Q 194

Query: 188 DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 247
           + +  + + Q+++AAI YSSGTTGRVK   + HR+ I  + G   L     +        
Sbjct: 195 EPLPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAREVR------ 254

Query: 248 ALCLLPLFHVFGFFMLFRSISEGNTLVLMR----KFDFEKMLRAVEKYRITYIPVSPPLI 307
            L   P+FH  GF  + + ++ G T V++     +     ++ A E++ +  +  SPP++
Sbjct: 255 TLLGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVV 314

Query: 308 VAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAART 367
           + M K        L +L+ + CGGAPL    I++F  +FP+V++  GYG TE+ G + R 
Sbjct: 315 LGMTKQRCR----LPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEAGGIS-RM 374

Query: 368 VGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAE 427
           +  EEC+  GS GR++EN+E KIVD  +G+ LP G +GELW+RGP +M GYVGD  A A 
Sbjct: 375 ISQEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANAT 434

Query: 428 TLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           T + EGWLKTGDLCY D DG L++VDRLKELIKYKAYQV
Sbjct: 435 TFNSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQV 461

BLAST of Csa1G050300.2 vs. Swiss-Prot
Match: 4CLL3_ARATH (4-coumarate--CoA ligase-like 3 OS=Arabidopsis thaliana GN=4CLL3 PE=2 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 1.0e-92
Identity = 196/465 (42.15%), Postives = 272/465 (58.49%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVS 65
           VD RSGFC     + S R PL LPP +  L V     +   SS P   T A ID+ +   
Sbjct: 10  VDPRSGFCKSNSTFYSKRNPLCLPP-NPSLDV-----TTFISSQPQRGTTAFIDASTGHR 69

Query: 66  VSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPIG 125
           ++++   R +  +   L        G V  ILSP S+ IPV+   ++SLG VV+ AN + 
Sbjct: 70  LTFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPNSIYIPVVCLSVMSLGAVVTTANTLN 129

Query: 126 SESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPE-----------FLSLMNES 185
           +  EI+ Q++   P + F TS  A K+    +  VL D  +            + +++E 
Sbjct: 130 TSGEISKQIAQSNPTLVFTTSQLAPKLAAA-ISVVLTDEEDEKRVELTSGVRVVGILSEM 189

Query: 186 NPSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDG 245
              +     + D ++NQ+D+A +LYSSGTTG  KGV+ SHRNL TA     + DK   D 
Sbjct: 190 MKKETSGQRVRD-RVNQDDTAMMLYSSGTTGTSKGVISSHRNL-TAYVAKYIDDKWKRD- 249

Query: 246 EIEPHPVALCLLPLFHVFGFFML-FRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVS 305
                 + +C +P+FH FG       S++ G+T+V++R+F  + M++AVEKY+ T + ++
Sbjct: 250 -----EIFVCTVPMFHSFGLLAFAMGSVASGSTVVILRRFGLDDMMQAVEKYKATILSLA 309

Query: 306 PPLIVAMAKS--ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESS 365
           PP++VAM     +L AKYDL+SL+ + CGGAPL KEV+D F  K+P V I QGY LTES 
Sbjct: 310 PPVLVAMINGADQLKAKYDLTSLRKVRCGGAPLSKEVMDSFLEKYPTVNIFQGYALTESH 369

Query: 366 GAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGD 425
           G+ A T   EE    G+VG LS  +EA+IVDP +G  +     GELW++GP I KGY G+
Sbjct: 370 GSGASTESVEESLKYGAVGLLSSGIEARIVDPDTGRVMGVNQPGELWLKGPSISKGYFGN 429

Query: 426 ERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           E AT ET++ EGWLK GDLCY D DG L++VDRLKELIKYK YQV
Sbjct: 430 EEATNETINLEGWLKLGDLCYIDEDGFLFVVDRLKELIKYKGYQV 459

BLAST of Csa1G050300.2 vs. TrEMBL
Match: A0A0A0LWL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G050300 PE=4 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 5.3e-258
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV
Sbjct: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 456

BLAST of Csa1G050300.2 vs. TrEMBL
Match: E5GBV5_CUCME (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 4.3e-244
Identity = 430/458 (93.89%), Postives = 441/458 (96.29%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIY SLRPPLSLPPLSQPLTV GHALSVLRSSPPPPNT ALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYF LLSLGVVVSP
Sbjct: 61  DSGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEIAHQVSLC P IAFA SSTASKIPRLPLGTVLIDSPEFLSLMNESN S+GV
Sbjct: 121 ANPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVL+SHRNLITAITG+QVLDKT VDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYR+TYIPVSPPL++AM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELA KYDLSSLQIL CGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES+GAA+RTVGP
Sbjct: 301 AKSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECS A SVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVIY 459
           PEGWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQ+++
Sbjct: 421 PEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQLMW 458

BLAST of Csa1G050300.2 vs. TrEMBL
Match: E5GBV5_CUCME (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 3.4e-188
Identity = 335/455 (73.63%), Postives = 382/455 (83.96%), Query Frame = 1

Query: 2   ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61
           A HS+D RSGFC  TKI++SLRPPLSLPPLSQPL+V  HA S+L+SS PPPN+  LIDS+
Sbjct: 497 APHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPNSTLLIDSN 556

Query: 62  SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121
           S + +SYA+FLRQIRNL SNLK+L S  NG VAFIL+PTSLQIPVLYF LLSLGVV+SPA
Sbjct: 557 SGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPA 616

Query: 122 NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181
           NP  S SEI+HQ+ L  P IAFATSSTASK+P L  GTV+IDSP FLS++ E+N SDG+ 
Sbjct: 617 NPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLT 676

Query: 182 DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241
           D    +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +G       + +GE++PHPV
Sbjct: 677 D----IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPV 736

Query: 242 ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301
           ALCLLPLFHVFGF ML R+IS G TLVLM +F+FE MLRAVEK+R+ YIPVSPPL+VAMA
Sbjct: 737 ALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMA 796

Query: 302 KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361
           KS+L AKYDLSSLQIL CGGAPLGKEVIDKFH K P+VEI QGYGLTES+  AART+ PE
Sbjct: 797 KSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPE 856

Query: 362 ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421
           E S   SVGRLS +MEAKIVDP+SGEAL P HKGELW+RGP IMKGYVGD++AT ETL P
Sbjct: 857 EISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDP 916

Query: 422 EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 917 DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 947

HSP 2 Score: 844.0 bits (2179), Expect = 9.0e-242
Identity = 429/456 (94.08%), Postives = 438/456 (96.05%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVD RSGFCSQTKIYNSLRPPLSLPPLSQPLT+  H LSVLRSS PPPN+  LIDS
Sbjct: 1   MATHSVDPRSGFCSQTKIYNSLRPPLSLPPLSQPLTIAEHVLSVLRSSSPPPNSAVLIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS +SVSYALFL QIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSGLSVSYALFLHQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEI HQVSLC PAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNES PSDGV
Sbjct: 121 ANPISSESEIVHQVSLCKPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESKPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAIL+SSGTTGR+KGVLISHRNLI+AI G++VLDKT VDGEIE HP
Sbjct: 181 NDGIFDLKINQNDSAAILFSSGTTGRLKGVLISHRNLISAINGVRVLDKTPVDGEIETHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISE +TLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISENHTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 456

BLAST of Csa1G050300.2 vs. TrEMBL
Match: A0A0A0LTS5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G050280 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.2e-187
Identity = 330/453 (72.85%), Postives = 380/453 (83.89%), Query Frame = 1

Query: 4   HSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSS 63
           HS+D RSGFC QTKI++SLRPPLSLPPLS PL+V+ HA S+L+SSPPPPN+  LID +S 
Sbjct: 13  HSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPPPNSTLLIDFNSG 72

Query: 64  VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANP 123
           + +SY +FLRQIRNL SNLK+L S  NGQVAFIL+PTSLQIPVLYF LLSLGVV+SPANP
Sbjct: 73  LHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFALLSLGVVLSPANP 132

Query: 124 IGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDG 183
             S SEI+HQ+ L  P IAFATSSTASK+P+L  GTVLIDSP F S++ E+N SDG+ + 
Sbjct: 133 TSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSMLTETNGSDGIPN- 192

Query: 184 IFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPVAL 243
              +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI    G   L   + +GE+EPHPV L
Sbjct: 193 ---IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGEMEPHPVVL 252

Query: 244 CLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMAKS 303
           C LPLFH+FGF ML R++S G+TLVLM +F+FE MLRAVEKYR+ YIPVSPPL+VAMAKS
Sbjct: 253 CPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPPLVVAMAKS 312

Query: 304 ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPEEC 363
           EL  KYDLSSLQ+L CGGAPLGKEVIDKFH K P VEI+QGYGLTES+  A+RT+ PEE 
Sbjct: 313 ELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGASRTMDPEEI 372

Query: 364 STAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHPEG 423
           S   SVGRLS NMEAKIVDP+SG+AL P HKGELW+RGP IMKGYVGD++AT ETL P+G
Sbjct: 373 SNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKATTETLDPDG 432

Query: 424 WLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           WLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 433 WLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 461

BLAST of Csa1G050300.2 vs. TrEMBL
Match: V7AZ93_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G167700g PE=4 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 8.7e-160
Identity = 295/453 (65.12%), Postives = 359/453 (79.25%), Query Frame = 1

Query: 5   SVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSV 64
           ++D  +GFCS ++I++SLRP + LPP SQPL++  +ALS+L ++    +  A+ID+ +  
Sbjct: 14  TIDPNTGFCSSSRIFHSLRPNVPLPPPSQPLSLADYALSLLPAAATDKS--AIIDATTGR 73

Query: 65  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 124
            +SY+LFLRQ+++L S++++L   S G VA IL+PTSL +PVLYF LLSLGV ++PANP+
Sbjct: 74  HLSYSLFLRQVKSLASSIQSLTPLSKGHVALILTPTSLHVPVLYFSLLSLGVTIAPANPL 133

Query: 125 GSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDGI 184
            S SE+AH + L  PAIAFATS+ A+ +P L  GTVLIDSP FLS+++ +  S     G+
Sbjct: 134 SSPSELAHLIQLTKPAIAFATSAVATNVPTLKFGTVLIDSPLFLSMLDANGDSYSRAPGV 193

Query: 185 FDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDG-EIEPHPVAL 244
              +++Q++SAAIL+SSGTTGRVKGVL+SHRNLI  I G   L K L D  E  PHPV+L
Sbjct: 194 ---ELSQSESAAILFSSGTTGRVKGVLLSHRNLIALIGGFCHLKKILQDSDEAVPHPVSL 253

Query: 245 CLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMAKS 304
             LPLFHVFGFFML RSI+ G TLVLM++FDFE ML+AVE+Y+I+ IPVSPPL+VA+AKS
Sbjct: 254 FTLPLFHVFGFFMLVRSIAMGETLVLMQRFDFEGMLKAVERYKISMIPVSPPLVVALAKS 313

Query: 305 ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPEEC 364
           EL  KYDLSSLQIL CGGAPLG++V D F  KFPNVEI QGYGLTES G AART+GPEE 
Sbjct: 314 ELVNKYDLSSLQILGCGGAPLGEQVADDFKAKFPNVEIAQGYGLTESGGGAARTLGPEEI 373

Query: 365 STAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHPEG 424
              GSVGRLSENMEAKIVDP +GEAL P  KGELWIRGP IMKGYVGDE+ATAETL  EG
Sbjct: 374 KRHGSVGRLSENMEAKIVDPVTGEALLPCQKGELWIRGPTIMKGYVGDEKATAETLDSEG 433

Query: 425 WLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           WLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 434 WLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 461

BLAST of Csa1G050300.2 vs. TAIR10
Match: AT5G63380.1 (AT5G63380.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 522.7 bits (1345), Expect = 2.3e-148
Identity = 277/461 (60.09%), Postives = 342/461 (74.19%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPA-------LI 65
           +D  SGF  +T IY+SLRP LSLPP+ QPL+    ALS+L  S PP            L+
Sbjct: 11  IDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLV 70

Query: 66  DSDSSVSVSYALFLRQIRNLTSNLKALY-SFSNGQVAFILSPTSLQIPVLYFVLLSLGVV 125
           +S S  +++Y   LR++R+L  +L+  + S ++  VAFILSP+SL IPVLY  L+S+GVV
Sbjct: 71  NSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV 130

Query: 126 VSPANPIGSESEIAHQVSLCIPAIAFATSSTASKIPR--LPLGTVLIDSPEFLSLMNESN 185
           VSPANPIGSESE++HQV +  P IAFATS T  K+    LPLGTVL+DS EFLS +N S+
Sbjct: 131 VSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSD 190

Query: 186 PSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGE 245
            S  VN   F +++NQ+D AAIL+SSGTTGRVKGVL++HRNLI +     V  +  +   
Sbjct: 191 -SSSVNP--FQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTA---VSHQRTLQDP 250

Query: 246 IEPHPVALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPP 305
           +    V L  LPLFHVFGF M+ R+IS G TLVL+ +F+ E M +AVEKY++T +PVSPP
Sbjct: 251 VNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPP 310

Query: 306 LIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAA 365
           LIVA+ KSEL  KYDL SL+ L CGGAPLGK++ ++F  KFP+V+I+QGYGLTESSG AA
Sbjct: 311 LIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAA 370

Query: 366 RTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERAT 425
            T GPEE    GSVGR+SENMEAKIVDPS+GE+LPPG  GELW+RGP IMKGYVG+E+A+
Sbjct: 371 STFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKAS 430

Query: 426 AETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           AET+  EGWLKTGDLCYFDS+  LYIVDRLKELIKYKAYQV
Sbjct: 431 AETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQV 465

BLAST of Csa1G050300.2 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 335.5 bits (859), Expect = 5.3e-92
Identity = 184/464 (39.66%), Postives = 278/464 (59.91%), Query Frame = 1

Query: 5   SVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSV 64
           SV+SRSGFC+    + S R P+ LPP +  L V     +   SS       A ID+ +  
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDV-----TTFISSQAHRGRIAFIDASTGQ 62

Query: 65  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 124
           ++++    R + ++   L  +     G V  +LSP S+  PV+   ++SLG +++  NP+
Sbjct: 63  NLTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPL 122

Query: 125 GSESEIAHQVSLCIPAIAFATS-------STASKIPRLPLGTVLIDSPEFLSLMNESNPS 184
            + +EIA Q+    P +AF TS       + A K+P + +    +DS   +  + E    
Sbjct: 123 NTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK 182

Query: 185 DGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVD--GE 244
           +   + + + +++Q+D+A +LYSSGTTG  KGV+ SHRNLI  +       +T+V+  G 
Sbjct: 183 EPSGNRVKE-RVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMV-------QTIVNRFGS 242

Query: 245 IEPHPVALCLLPLFHVFGFFMLFRSI-SEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSP 304
            +     +C +P+FH++G       + + G+T++++ KF+  +M+ A+ KY+ T +P+ P
Sbjct: 243 DDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVP 302

Query: 305 PLIVAMAKS--ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSG 364
           P++VAM     ++ AKYDLSS+  + CGGAPL KEV + F  K+P V+I+QGYGLTES+G
Sbjct: 303 PILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG 362

Query: 365 AAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDE 424
             A T   EE    G+ G+LS +ME +IVDP +G+ L P   GELW++GP IMKGY  +E
Sbjct: 363 IGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNE 422

Query: 425 RATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
            AT+ TL  EGWL+TGDLCY D DG +++VDRLKELIKYK YQV
Sbjct: 423 EATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQV 451

BLAST of Csa1G050300.2 vs. TAIR10
Match: AT1G20500.1 (AT1G20500.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 330.1 bits (845), Expect = 2.2e-90
Identity = 185/464 (39.87%), Postives = 269/464 (57.97%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPP-LSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSV 65
           VD RSGFC     + S R PLSLPP LS+ +T          SS P     A ID+ +  
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTT-------FISSQPHRGKTAFIDAATGQ 69

Query: 66  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 125
            ++++   R +  +   L        G V  ILSP S+ IPV+   ++SLG V + AN +
Sbjct: 70  CLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTL 129

Query: 126 GSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEF---------LSLMNESN 185
            +  EI+ Q++   P + F T   A K+P + +  VL D   +         + +++E  
Sbjct: 130 NTSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMV 189

Query: 186 PSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGE 245
             +     + D ++NQ+D+A +LYSSGTTG  KGV+ SHRNL   +          +   
Sbjct: 190 KKEPSGQRVRD-RVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVA-------RFISDN 249

Query: 246 IEPHPVALCLLPLFHVFGFFML-FRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSP 305
           ++   + +C +P+FH +G       +++ G+T+V++R+F    M+ AVEK+R T + ++P
Sbjct: 250 LKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAP 309

Query: 306 PLIVAMAKSE--LAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSG 365
           P++VAM      + AKYDLSSL+ + CGGAPL KEV + F  K+P V+I+QGY LTES+G
Sbjct: 310 PVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNG 369

Query: 366 AAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDE 425
             A T   EE    G+ G L+ ++EA+IVDP++G  +     GELW++GP I KGY  ++
Sbjct: 370 GGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQ 429

Query: 426 RATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
            AT ET++ EGWLKTGDLCY D DG L++VDRLKELIKYK YQV
Sbjct: 430 EATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQV 457

BLAST of Csa1G050300.2 vs. TAIR10
Match: AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 321.2 bits (822), Expect = 1.0e-87
Identity = 190/474 (40.08%), Postives = 264/474 (55.70%), Query Frame = 1

Query: 3   THSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDS 62
           T +VD +SGFC  T I+ S R P++LPP +Q L V         +S P       +D+ +
Sbjct: 18  TLAVDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVT 77

Query: 63  SVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPAN 122
              +S+      +  +   L AL     G V  ILSP S+  P++   ++SLG +++ AN
Sbjct: 78  GRRLSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTAN 137

Query: 123 PIGSESEIAHQVSLCIPAIAFATSSTASKIP---RLPLGTVLIDSPEF------------ 182
           PI +  EI+ Q+    P +AF T    SK+       L  VL+D                
Sbjct: 138 PINTSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLV 197

Query: 183 --LSLMNESNPSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQ 242
             L  M E+ PS+         ++NQ+D+AA+LYSSGTTG  KGV++SHRNLI  +   +
Sbjct: 198 GRLETMIETEPSESR----VKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYR 257

Query: 243 VLDKTLVDGEIEPHPVALCLLPLFHVFGFFMLFRS-ISEGNTLVLMRKFDFEKMLRAVEK 302
                            +C +P+ H+FGF       I+ G T+V++ KFD  K+L AVE 
Sbjct: 258 A--------RFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVET 317

Query: 303 YRITYIPVSPPLIVAMAK--SELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEII 362
           +R +Y+ + PP++VAM    +E+ +KYDLSSL  +  GGAPL +EV +KF   +P V+I+
Sbjct: 318 HRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKIL 377

Query: 363 QGYGLTESSGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGP 422
           QGYGLTES+  AA     EE    G+ G L+ N+E KIVDP +G  L     GELWIR P
Sbjct: 378 QGYGLTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSP 437

Query: 423 GIMKGYVGDERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
            +MKGY  ++ ATA T+  EGWLKTGDLCY D DG +++VDRLKELIK   YQV
Sbjct: 438 TVMKGYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQV 472

BLAST of Csa1G050300.2 vs. TAIR10
Match: AT5G38120.1 (AT5G38120.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 303.5 bits (776), Expect = 2.2e-82
Identity = 177/469 (37.74%), Postives = 261/469 (55.65%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVS 65
           +D R+GFC+    + S R PL+LP   + L +     +   SS       A ID+ +   
Sbjct: 11  IDPRNGFCTSNSTFYSKRKPLALPS-KESLDI-----TTFISSQTYRGKTAFIDAATDHR 70

Query: 66  VSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPIG 125
           +S++     +  +   L        G V  +LSP ++ IP++   ++SLG V++ ANP+ 
Sbjct: 71  ISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLN 130

Query: 126 SESEIAHQVSLCIPAIAFATSSTASKIP---------------RLPLGTVLIDSPEFLSL 185
           + SEI  Q++   P +AF T   A KI                R+P G  ++ +     +
Sbjct: 131 TASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVGN--LTEM 190

Query: 186 MNESNPSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKT 245
           M +      V +     +++++D+A +LYSSGTTGR KGV  SH NLI  +       + 
Sbjct: 191 MKKEPSGQAVRN-----QVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVA------RY 250

Query: 246 LVDGEIEPHPVALCLLPLFHVFGFF-MLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITY 305
           + +   +P    +C +PLFH FG    +  +++ G T+V++ +FD  +M+ AVEKYR T 
Sbjct: 251 IAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATT 310

Query: 306 IPVSPPLIVAMAKS--ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGL 365
           + + PP++V M     ++  KYD+S L+ + CGGAPL KEV   F  K+P V++ QGY L
Sbjct: 311 LILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYAL 370

Query: 366 TESSGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKG 425
           TES+GA A     EE    G+VG LS  +EA+IVDP++G+ +     GELW++GP I KG
Sbjct: 371 TESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKG 430

Query: 426 YVGDERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           Y  +E    E +  EGWLKTGDLCY D+DG L+IVDRLKELIKYK YQV
Sbjct: 431 YFRNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQV 457

BLAST of Csa1G050300.2 vs. NCBI nr
Match: gi|778658041|ref|XP_004152591.2| (PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis sativus])

HSP 1 Score: 897.9 bits (2319), Expect = 7.6e-258
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV
Sbjct: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 456

BLAST of Csa1G050300.2 vs. NCBI nr
Match: gi|659068783|ref|XP_008446209.1| (PREDICTED: uncharacterized protein LOC103489008 [Cucumis melo])

HSP 1 Score: 851.7 bits (2199), Expect = 6.2e-244
Identity = 431/456 (94.52%), Postives = 439/456 (96.27%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIY SLRPPLSLPPLSQPLTV GHALSVLRSSPPPPNT ALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYF LLSLGVVVSP
Sbjct: 61  DSGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEIAHQVSLC P IAFA SSTASKIPRLPLGTVLIDSPEFLSLMNESN S+GV
Sbjct: 121 ANPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVL+SHRNLITAITG+QVLDKT VDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYR+TYIPVSPPL++AM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELA KYDLSSLQIL CGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES+GAA+RTVGP
Sbjct: 301 AKSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECS A SVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 421 PEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 456

BLAST of Csa1G050300.2 vs. NCBI nr
Match: gi|659068783|ref|XP_008446209.1| (PREDICTED: uncharacterized protein LOC103489008 [Cucumis melo])

HSP 1 Score: 666.0 bits (1717), Expect = 4.8e-188
Identity = 335/455 (73.63%), Postives = 382/455 (83.96%), Query Frame = 1

Query: 2    ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61
            A HS+D RSGFC  TKI++SLRPPLSLPPLSQPL+V  HA S+L+SS PPPN+  LIDS+
Sbjct: 576  APHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPNSTLLIDSN 635

Query: 62   SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121
            S + +SYA+FLRQIRNL SNLK+L S  NG VAFIL+PTSLQIPVLYF LLSLGVV+SPA
Sbjct: 636  SGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPA 695

Query: 122  NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181
            NP  S SEI+HQ+ L  P IAFATSSTASK+P L  GTV+IDSP FLS++ E+N SDG+ 
Sbjct: 696  NPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLT 755

Query: 182  DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241
            D    +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +G       + +GE++PHPV
Sbjct: 756  D----IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPV 815

Query: 242  ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301
            ALCLLPLFHVFGF ML R+IS G TLVLM +F+FE MLRAVEK+R+ YIPVSPPL+VAMA
Sbjct: 816  ALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMA 875

Query: 302  KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361
            KS+L AKYDLSSLQIL CGGAPLGKEVIDKFH K P+VEI QGYGLTES+  AART+ PE
Sbjct: 876  KSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPE 935

Query: 362  ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421
            E S   SVGRLS +MEAKIVDP+SGEAL P HKGELW+RGP IMKGYVGD++AT ETL P
Sbjct: 936  EISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDP 995

Query: 422  EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
            +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 996  DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 1026

HSP 2 Score: 851.7 bits (2199), Expect = 6.2e-244
Identity = 430/458 (93.89%), Postives = 441/458 (96.29%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIY SLRPPLSLPPLSQPLTV GHALSVLRSSPPPPNT ALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYF LLSLGVVVSP
Sbjct: 61  DSGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEIAHQVSLC P IAFA SSTASKIPRLPLGTVLIDSPEFLSLMNESN S+GV
Sbjct: 121 ANPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVL+SHRNLITAITG+QVLDKT VDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYR+TYIPVSPPL++AM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELA KYDLSSLQIL CGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES+GAA+RTVGP
Sbjct: 301 AKSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECS A SVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVIY 459
           PEGWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQ+++
Sbjct: 421 PEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQLMW 458

BLAST of Csa1G050300.2 vs. NCBI nr
Match: gi|307136108|gb|ADN33954.1| (4-coumarate-CoA ligase [Cucumis melo subsp. melo])

HSP 1 Score: 666.0 bits (1717), Expect = 4.8e-188
Identity = 335/455 (73.63%), Postives = 382/455 (83.96%), Query Frame = 1

Query: 2   ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61
           A HS+D RSGFC  TKI++SLRPPLSLPPLSQPL+V  HA S+L+SS PPPN+  LIDS+
Sbjct: 497 APHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPNSTLLIDSN 556

Query: 62  SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121
           S + +SYA+FLRQIRNL SNLK+L S  NG VAFIL+PTSLQIPVLYF LLSLGVV+SPA
Sbjct: 557 SGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPA 616

Query: 122 NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181
           NP  S SEI+HQ+ L  P IAFATSSTASK+P L  GTV+IDSP FLS++ E+N SDG+ 
Sbjct: 617 NPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLT 676

Query: 182 DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241
           D    +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +G       + +GE++PHPV
Sbjct: 677 D----IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPV 736

Query: 242 ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301
           ALCLLPLFHVFGF ML R+IS G TLVLM +F+FE MLRAVEK+R+ YIPVSPPL+VAMA
Sbjct: 737 ALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMA 796

Query: 302 KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361
           KS+L AKYDLSSLQIL CGGAPLGKEVIDKFH K P+VEI QGYGLTES+  AART+ PE
Sbjct: 797 KSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPE 856

Query: 362 ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421
           E S   SVGRLS +MEAKIVDP+SGEAL P HKGELW+RGP IMKGYVGD++AT ETL P
Sbjct: 857 EISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDP 916

Query: 422 EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 917 DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 947

HSP 2 Score: 844.0 bits (2179), Expect = 1.3e-241
Identity = 429/456 (94.08%), Postives = 438/456 (96.05%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVD RSGFCSQTKIYNSLRPPLSLPPLSQPLT+  H LSVLRSS PPPN+  LIDS
Sbjct: 1   MATHSVDPRSGFCSQTKIYNSLRPPLSLPPLSQPLTIAEHVLSVLRSSSPPPNSAVLIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS +SVSYALFL QIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSGLSVSYALFLHQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEI HQVSLC PAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNES PSDGV
Sbjct: 121 ANPISSESEIVHQVSLCKPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESKPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAIL+SSGTTGR+KGVLISHRNLI+AI G++VLDKT VDGEIE HP
Sbjct: 181 NDGIFDLKINQNDSAAILFSSGTTGRLKGVLISHRNLISAINGVRVLDKTPVDGEIETHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISE +TLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISENHTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 456

BLAST of Csa1G050300.2 vs. NCBI nr
Match: gi|778658037|ref|XP_011652003.1| (PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis sativus])

HSP 1 Score: 663.3 bits (1710), Expect = 3.1e-187
Identity = 330/453 (72.85%), Postives = 380/453 (83.89%), Query Frame = 1

Query: 4   HSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSS 63
           HS+D RSGFC QTKI++SLRPPLSLPPLS PL+V+ HA S+L+SSPPPPN+  LID +S 
Sbjct: 13  HSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPPPNSTLLIDFNSG 72

Query: 64  VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANP 123
           + +SY +FLRQIRNL SNLK+L S  NGQVAFIL+PTSLQIPVLYF LLSLGVV+SPANP
Sbjct: 73  LHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFALLSLGVVLSPANP 132

Query: 124 IGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDG 183
             S SEI+HQ+ L  P IAFATSSTASK+P+L  GTVLIDSP F S++ E+N SDG+ + 
Sbjct: 133 TSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSMLTETNGSDGIPN- 192

Query: 184 IFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPVAL 243
              +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI    G   L   + +GE+EPHPV L
Sbjct: 193 ---IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGEMEPHPVVL 252

Query: 244 CLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMAKS 303
           C LPLFH+FGF ML R++S G+TLVLM +F+FE MLRAVEKYR+ YIPVSPPL+VAMAKS
Sbjct: 253 CPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPPLVVAMAKS 312

Query: 304 ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPEEC 363
           EL  KYDLSSLQ+L CGGAPLGKEVIDKFH K P VEI+QGYGLTES+  A+RT+ PEE 
Sbjct: 313 ELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGASRTMDPEEI 372

Query: 364 STAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHPEG 423
           S   SVGRLS NMEAKIVDP+SG+AL P HKGELW+RGP IMKGYVGD++AT ETL P+G
Sbjct: 373 SNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKATTETLDPDG 432

Query: 424 WLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           WLKTGDLCYFDSDG LYIVDRLKELIKYKAYQV
Sbjct: 433 WLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQV 461