Csa1G050300 (gene) Cucumber (Chinese Long) v2

NameCsa1G050300
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
Description4-coumarate-CoA ligase; contains IPR000873 (AMP-dependent synthetase/ligase), IPR025110 (Domain of unknown function DUF4009)
LocationChr1 : 5850954 .. 5853840 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAATTTGCGTACTTGGATCATCTTCAGCCACGAATTTCATTCATATTTTTCCTTCACAAATCTGAATTTCACATCAATGGCTACCCATTCTGTTGATTCTCGGAGCGGTTTCTGTTCTCAAACCAAGATTTACAACAGTCTCCGACCACCGTTATCGCTTCCCCCGTTATCTCAACCACTTACCGTTGTTGGGCATGCCCTCTCTGTTCTCCGATCATCTCCTCCTCCGCCCAACACTCCTGCTCTCATCGACTCCGACTCCAGTGTCAGTGTCTCTTACGCTCTTTTCCTCCGTCAAATTCGAAACCTCACCTCTAACCTTAAAGCTCTTTATTCCTTCTCCAATGGTCAAGTGGCGTTCATTCTTTCACCCACTTCTCTGCAAATCCCTGTGTTGTATTTCGTTTTATTATCTCTCGGTGTTGTTGTCTCTCCGGCTAATCCTATCGGTTCCGAATCGGAGATTGCTCACCAGGTTAGTCTCTGTATACCAGCCATTGCCTTCGCTACTTCCTCAACGGCGTCTAAGATCCCGAGGCTTCCACTTGGCACTGTATTGATAGATTCACCGGAGTTTCTTTCTTTGATGAATGAAAGTAATCCATCGGATGGAGTAAACGATGGAATTTTCGATCTCAAAATCAATCAAAACGACTCTGCGGCGATTCTGTACTCTTCAGGGACTACCGGGCGAGTGAAAGGCGTTTTAATCTCTCACCGGAACCTCATAACGGCGATCACTGGGCTTCAAGTCTTAGATAAAACGCTCGTTGATGGAGAGATTGAGCCACATCCCGTTGCTCTTTGTCTGTTACCTCTGTTTCATGTTTTCGGGTTCTTTATGTTATTTCGATCGATTTCAGAGGGCAATACATTGGTTTTGATGCGTAAGTTCGATTTCGAGAAGATGTTAAGAGCAGTGGAGAAGTATAGGATTACATACATTCCGGTCTCTCCGCCACTGATTGTGGCCATGGCCAAATCGGAGCTGGCAGCGAAGTATGATCTCAGTTCTCTTCAAATTTTGGCATGCGGCGGTGCTCCTCTTGGTAAAGAAGTCATCGATAAATTCCATGTAAAGTTCCCCAACGTAGAAATTATTCAGGTTAGTAACAGACACTTTCCCTTCTTAGTTACTCGTTACAATGTTCTTTGCTTTCTCATATCATATAAATGCGATGAACATAACATTCTGTCAATTCCTAGTTTGTAATCTGTTCTCAATTTTGTAAGTCCATTCCAAATTATCAATTCAAACTTTTCCACTTAAGTGTTCTGATTCCATGAGTTTTTTTTGCTATGAGTATACTTTTAAAGCTTGTGGAATTCTCACAATTAAGTTCAGATAGGCAAAGTTTTTAACCAAAATTTGTAACTTCACACCAAAGTTCAAATATGCAGTTTCAATGGTTGACCATGTGTTTTAATACTCTCTTTTAAGTCACCAACCCAAAGGATGAAGTTGATTGTTAAAAGACAAATTTAATATTATATTATTTAACACAATTCTAATCCATCCAATATCCTAGGATGGTTATAAGATAGTTAAGGAAGCACTTAAATCTATTTGAAGTTAAAAACAAGTAAAATGATAACAAAGAATGATGCATGGTTTTGTTGAATAGGGATATGGATTGACAGAGAGTTCAGGAGCAGCAGCAAGAACTGTGGGGCCTGAAGAATGCAGTACAGCAGGTTCCGTCGGTCGCCTATCCGAAAATATGGAAGCCAAAATAGTCGATCCTTCATCTGGAGAAGCTTTACCTCCTGGTCACAAAGGAGAACTTTGGATACGAGGTCCAGGAATCATGAAAGGTTTGCAATATTCTCTTCTATATGTGTTTTCTTCTATACTTCAGTATGAGAATGAAATCTAGCAAGTGAAGTATTATAAATTATTAATTGTATAATACTGAATTTGTCCCCATTCCTCTCTTCATTAGAGAGCTCTGTTTGAGAATAGGATGTTTTGCTACAAGGCATTAATCATGATGTTGTTTATTGTGTGATGAACATATGAAATGGTGTTTGATGCAGGTTATGTTGGAGATGAAAGGGCAACTGCTGAAACCTTGCATCCTGAGGGATGGCTAAAGACTGGTGACCTTTGTTACTTTGATTCTGATGGATTACTCTACATCGTTGATAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTGATTTATTCACTCCCAGTTCTTGACAAGATAAGAATATGACCATAAAATGCCATAAGTTATTATTGTTGCCTTAGTGTATCTTTAGTAATCGGAAGTCCTTTTGTTTGTTTTAAACAAATTCCAGGTCCCACCTGCCGAATTGGAACATTTGCTTCAATCCCACCCCGAAATCCTTGATGCTGCTGTGATACCGTGTGTATTCTTTGCTCTCTTCTATATTTGATTTTGAATTAATAAACAAACTTGTTTGATTGTGATTTTAACTACTTTATAACAACTTCGAAACATAAGTTTTACTCAAATTTTCTGATATTATTTTCACTCCTCTTGAGCAGCTATCCTGATGAAGACGCGGGACAAATTCCATTAGCTTACGTGGTAAGACAACTTGGAAGCAATATCAACGAAGCTCAAGTCATGGATTTCATTGCAAAAAAGGTACCTTTCAACTCGAAATCAACTTTAACTTTCACTTAGAAAGTCATTTCAAACTTATCCCGAACTATTCTAATATGGTTGTAGGTTGCACCATACAAGAAAATCCGAAGAGTTTCTTTTGTGAATGCTATCCCTAAATCGCCTGCAGGAAAGATTCTTAGGAGGGAACTTGTTAAACATGCTCTCTCCCAAGGTTCAAGCAAGCTATAATAAAGGGAAATGGAATTGGAATTGGAATTGTACATTCTTAATTTTAATCCT

mRNA sequence

ATGGCTACCCATTCTGTTGATTCTCGGAGCGGTTTCTGTTCTCAAACCAAGATTTACAACAGTCTCCGACCACCGTTATCGCTTCCCCCGTTATCTCAACCACTTACCGTTGTTGGGCATGCCCTCTCTGTTCTCCGATCATCTCCTCCTCCGCCCAACACTCCTGCTCTCATCGACTCCGACTCCAGTGTCAGTGTCTCTTACGCTCTTTTCCTCCGTCAAATTCGAAACCTCACCTCTAACCTTAAAGCTCTTTATTCCTTCTCCAATGGTCAAGTGGCGTTCATTCTTTCACCCACTTCTCTGCAAATCCCTGTGTTGTATTTCGTTTTATTATCTCTCGGTGTTGTTGTCTCTCCGGCTAATCCTATCGGTTCCGAATCGGAGATTGCTCACCAGGTTAGTCTCTGTATACCAGCCATTGCCTTCGCTACTTCCTCAACGGCGTCTAAGATCCCGAGGCTTCCACTTGGCACTGTATTGATAGATTCACCGGAGTTTCTTTCTTTGATGAATGAAAGTAATCCATCGGATGGAGTAAACGATGGAATTTTCGATCTCAAAATCAATCAAAACGACTCTGCGGCGATTCTGTACTCTTCAGGGACTACCGGGCGAGTGAAAGGCGTTTTAATCTCTCACCGGAACCTCATAACGGCGATCACTGGGCTTCAAGTCTTAGATAAAACGCTCGTTGATGGAGAGATTGAGCCACATCCCGTTGCTCTTTGTCTGTTACCTCTGTTTCATGTTTTCGGGTTCTTTATGTTATTTCGATCGATTTCAGAGGGCAATACATTGGTTTTGATGCGTAAGTTCGATTTCGAGAAGATGTTAAGAGCAGTGGAGAAGTATAGGATTACATACATTCCGGTCTCTCCGCCACTGATTGTGGCCATGGCCAAATCGGAGCTGGCAGCGAAGTATGATCTCAGTTCTCTTCAAATTTTGGCATGCGGCGGTGCTCCTCTTGGTAAAGAAGTCATCGATAAATTCCATGTAAAGTTCCCCAACGTAGAAATTATTCAGGGATATGGATTGACAGAGAGTTCAGGAGCAGCAGCAAGAACTGTGGGGCCTGAAGAATGCAGTACAGCAGGTTCCGTCGGTCGCCTATCCGAAAATATGGAAGCCAAAATAGTCGATCCTTCATCTGGAGAAGCTTTACCTCCTGGTCACAAAGGAGAACTTTGGATACGAGGTCCAGGAATCATGAAAGGTTATGTTGGAGATGAAAGGGCAACTGCTGAAACCTTGCATCCTGAGGGATGGCTAAAGACTGGTGACCTTTGTTACTTTGATTCTGATGGATTACTCTACATCGTTGATAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTGATTTATTCACTCCCAGTTCTTGACAAGATAAGAATATGA

Coding sequence (CDS)

ATGGCTACCCATTCTGTTGATTCTCGGAGCGGTTTCTGTTCTCAAACCAAGATTTACAACAGTCTCCGACCACCGTTATCGCTTCCCCCGTTATCTCAACCACTTACCGTTGTTGGGCATGCCCTCTCTGTTCTCCGATCATCTCCTCCTCCGCCCAACACTCCTGCTCTCATCGACTCCGACTCCAGTGTCAGTGTCTCTTACGCTCTTTTCCTCCGTCAAATTCGAAACCTCACCTCTAACCTTAAAGCTCTTTATTCCTTCTCCAATGGTCAAGTGGCGTTCATTCTTTCACCCACTTCTCTGCAAATCCCTGTGTTGTATTTCGTTTTATTATCTCTCGGTGTTGTTGTCTCTCCGGCTAATCCTATCGGTTCCGAATCGGAGATTGCTCACCAGGTTAGTCTCTGTATACCAGCCATTGCCTTCGCTACTTCCTCAACGGCGTCTAAGATCCCGAGGCTTCCACTTGGCACTGTATTGATAGATTCACCGGAGTTTCTTTCTTTGATGAATGAAAGTAATCCATCGGATGGAGTAAACGATGGAATTTTCGATCTCAAAATCAATCAAAACGACTCTGCGGCGATTCTGTACTCTTCAGGGACTACCGGGCGAGTGAAAGGCGTTTTAATCTCTCACCGGAACCTCATAACGGCGATCACTGGGCTTCAAGTCTTAGATAAAACGCTCGTTGATGGAGAGATTGAGCCACATCCCGTTGCTCTTTGTCTGTTACCTCTGTTTCATGTTTTCGGGTTCTTTATGTTATTTCGATCGATTTCAGAGGGCAATACATTGGTTTTGATGCGTAAGTTCGATTTCGAGAAGATGTTAAGAGCAGTGGAGAAGTATAGGATTACATACATTCCGGTCTCTCCGCCACTGATTGTGGCCATGGCCAAATCGGAGCTGGCAGCGAAGTATGATCTCAGTTCTCTTCAAATTTTGGCATGCGGCGGTGCTCCTCTTGGTAAAGAAGTCATCGATAAATTCCATGTAAAGTTCCCCAACGTAGAAATTATTCAGGGATATGGATTGACAGAGAGTTCAGGAGCAGCAGCAAGAACTGTGGGGCCTGAAGAATGCAGTACAGCAGGTTCCGTCGGTCGCCTATCCGAAAATATGGAAGCCAAAATAGTCGATCCTTCATCTGGAGAAGCTTTACCTCCTGGTCACAAAGGAGAACTTTGGATACGAGGTCCAGGAATCATGAAAGGTTATGTTGGAGATGAAAGGGCAACTGCTGAAACCTTGCATCCTGAGGGATGGCTAAAGACTGGTGACCTTTGTTACTTTGATTCTGATGGATTACTCTACATCGTTGATAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTGATTTATTCACTCCCAGTTCTTGACAAGATAAGAATATGA

Protein sequence

MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPVALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVIYSLPVLDKIRI*
BLAST of Csa1G050300 vs. Swiss-Prot
Match: 4CLL9_ARATH (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 664.1 bits (1712), Expect = 1.4e-189
Identity = 341/558 (61.11%), Postives = 429/558 (76.88%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPA-------LI 65
           +D  SGF  +T IY+SLRP LSLPP+ QPL+    ALS+L  S PP            L+
Sbjct: 11  IDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLV 70

Query: 66  DSDSSVSVSYALFLRQIRNLTSNLKALY-SFSNGQVAFILSPTSLQIPVLYFVLLSLGVV 125
           +S S  +++Y   LR++R+L  +L+  + S ++  VAFILSP+SL IPVLY  L+S+GVV
Sbjct: 71  NSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV 130

Query: 126 VSPANPIGSESEIAHQVSLCIPAIAFATSSTASKIPR--LPLGTVLIDSPEFLSLMNESN 185
           VSPANPIGSESE++HQV +  P IAFATS T  K+    LPLGTVL+DS EFLS +N S+
Sbjct: 131 VSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSD 190

Query: 186 PSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGE 245
            S  VN   F +++NQ+D AAIL+SSGTTGRVKGVL++HRNLI +     V  +  +   
Sbjct: 191 -SSSVNP--FQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTA---VSHQRTLQDP 250

Query: 246 IEPHPVALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPP 305
           +    V L  LPLFHVFGF M+ R+IS G TLVL+ +F+ E M +AVEKY++T +PVSPP
Sbjct: 251 VNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPP 310

Query: 306 LIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAA 365
           LIVA+ KSEL  KYDL SL+ L CGGAPLGK++ ++F  KFP+V+I+QGYGLTESSG AA
Sbjct: 311 LIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAA 370

Query: 366 RTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERAT 425
            T GPEE    GSVGR+SENMEAKIVDPS+GE+LPPG  GELW+RGP IMKGYVG+E+A+
Sbjct: 371 STFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKAS 430

Query: 426 AETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAA 485
           AET+  EGWLKTGDLCYFDS+  LYIVDRLKELIKYKAYQVPP ELE +L S+P+++DAA
Sbjct: 431 AETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAA 490

Query: 486 VIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGK 545
           V+P+PDEDAG+IP+A++VR+ GSN+NEAQ++DF+AK+V PYKK+RRV+F+NAIPK+PAGK
Sbjct: 491 VVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAIPKNPAGK 550

Query: 546 ILRRELVKHALSQGSSKL 554
           ILRREL K A+   +SKL
Sbjct: 551 ILRRELTKIAVDGNASKL 562

BLAST of Csa1G050300 vs. Swiss-Prot
Match: 4CLL5_ORYSJ (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-161
Identity = 306/556 (55.04%), Postives = 388/556 (69.78%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVS 65
           +DSRSGFC+ T+I++S R P  LPP S P+T   +A S+L SS  P   PAL+D+ + ++
Sbjct: 15  IDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLP-GRPALVDAATGIA 74

Query: 66  VSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPIG 125
           +SY  FL  +R+L   L        G VA +++P+ L++PVL F L+S+G VVSPANP+ 
Sbjct: 75  ISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFALMSIGAVVSPANPVS 134

Query: 126 SESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDGI- 185
           +  E AHQV+L  P +AFA    A+K+P   +  V+I S E+  L      SDG      
Sbjct: 135 TPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEYGRLA----ASDGRRAAAP 194

Query: 186 FDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEI-----EPH 245
             + + Q+D+AA+LYSSGTTGRVK V I+HRNLI A+  L   ++  V  E      EP 
Sbjct: 195 AAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLI-ALMSLHADNREKVAREAAEAGEEPP 254

Query: 246 PVALCLLP--LFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLI 305
           P A+ LLP  LFHVFGF M+ RS+S G T VLM +FDF   LRA+E+YR+T +P +PP++
Sbjct: 255 PPAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVTLLPAAPPVL 314

Query: 306 VAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAART 365
           VAM K E A + DLSSL ++  GGAPLG+EV ++F   FPNVE++QGYGLTESSGA A T
Sbjct: 315 VAMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLTESSGAVAAT 374

Query: 366 VGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAE 425
           VGPEE    GSVG+L  +++AKIVDPS+G                     YVGD+ ATA 
Sbjct: 375 VGPEESKAYGSVGKLGSHLQAKIVDPSTG---------------------YVGDDEATAA 434

Query: 426 TLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVI 485
           T+  EGWLKTGDLCYF+ DG LYIVDRLKELIKYK YQVPPAELEH+LQSHP I DAAVI
Sbjct: 435 TVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSHPGIADAAVI 494

Query: 486 PYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKIL 545
           PYPDE+AG++P+A++VRQ GSNI + QVMD++AK+VAPYKK+RRV+FV AIPKSPAGKIL
Sbjct: 495 PYPDEEAGELPMAFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVTAIPKSPAGKIL 542

Query: 546 RRELVKHALSQGSSKL 554
           RRELV+ ALS G+SKL
Sbjct: 555 RRELVQQALSMGASKL 542

BLAST of Csa1G050300 vs. Swiss-Prot
Match: 4CLL6_ORYSJ (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 566.2 bits (1458), Expect = 3.9e-160
Identity = 301/560 (53.75%), Postives = 383/560 (68.39%), Query Frame = 1

Query: 8   SRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPP---PPNTPALIDSDSSV 67
           SRSGFC+ T+ ++SLR    LPP   PLTV  +A S+L S+PP       PAL+D+ + +
Sbjct: 40  SRSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGI 99

Query: 68  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 127
           +VSY  F+ ++R L   L        G VA ++SP+ L + VLYF L+S+GVVVSPANP 
Sbjct: 100 AVSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPA 159

Query: 128 GSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDGI 187
            +  E AHQV L  PAIAF     A+++PR  +  V+I S  F  L + S          
Sbjct: 160 STADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVFDRLASASAAGGWAAPPA 219

Query: 188 FDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVD------GEIEP 247
             +K  Q  +AA+LYSSGTTGRVK V I+HRNLI  I+    + +T+        G+ +P
Sbjct: 220 VAMK--QPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKP 279

Query: 248 HP--------VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYI 307
            P        V L  LPLFHV GF +L R+IS G T V+MR+FD     RAVE+YR+T +
Sbjct: 280 PPPSPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKL 339

Query: 308 PVSPPLIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES 367
             +PP++VA+ KS+ A + DLSSL  +  GGAPLG+EV  +F   FP+V+I+Q YGLTES
Sbjct: 340 SAAPPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTES 399

Query: 368 SGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVG 427
           +G  A   GPEE +  GSVGRL+  ++AKIVD ++GE L PG +GELWIRGP +MKGYVG
Sbjct: 400 TGPVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVG 459

Query: 428 DERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPE 487
           D  ATA T+ P+GWLKTGDLCYF+ DG LY+VDRLKELIKYK YQVPPAELEH+LQS PE
Sbjct: 460 DPEATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPE 519

Query: 488 ILDAAVIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPK 547
           I DAAV+PYPDE+AGQ+P+A+VVRQ G+ + E QVM+ +AK VAPYKK+RRV+FVNAIPK
Sbjct: 520 IADAAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVRRVAFVNAIPK 579

Query: 548 SPAGKILRRELVKHALSQGS 551
           SPAGKILRRELV  A++  S
Sbjct: 580 SPAGKILRRELVLQAMASTS 596

BLAST of Csa1G050300 vs. Swiss-Prot
Match: 4CLL7_ORYSJ (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.6e-136
Identity = 257/547 (46.98%), Postives = 355/547 (64.90%), Query Frame = 1

Query: 8   SRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVL------RSSPPPPNTPALIDSD 67
           +R+G+C+ TK + SLRPP+ LPP   PL+    A S+L       SS   P  PAL+D+ 
Sbjct: 15  TRTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSSSSSSLLPANPALVDAA 74

Query: 68  SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 127
           +  +VS+  FL ++R L   L++      G VAF+L+P  L +PVLYF LLS+G VVSPA
Sbjct: 75  TGEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPA 134

Query: 128 NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 187
           NP  + +E++  VSL   ++AFA SSTA+K+P      VL+DSP F SL+ +   + G  
Sbjct: 135 NPALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQG-Q 194

Query: 188 DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 247
           + +  + + Q+++AAI YSSGTTGRVK   + HR+ I  + G   L     +        
Sbjct: 195 EPLPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAREVR------ 254

Query: 248 ALCLLPLFHVFGFFMLFRSISEGNTLVLMR----KFDFEKMLRAVEKYRITYIPVSPPLI 307
            L   P+FH  GF  + + ++ G T V++     +     ++ A E++ +  +  SPP++
Sbjct: 255 TLLGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVV 314

Query: 308 VAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAART 367
           + M K        L +L+ + CGGAPL    I++F  +FP+V++  GYG TE+ G + R 
Sbjct: 315 LGMTKQRCR----LPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEAGGIS-RM 374

Query: 368 VGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAE 427
           +  EEC+  GS GR++EN+E KIVD  +G+ LP G +GELW+RGP +M GYVGD  A A 
Sbjct: 375 ISQEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANAT 434

Query: 428 TLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVI 487
           T + EGWLKTGDLCY D DG L++VDRLKELIKYKAYQVPPAELE +L S P+I+DAAV+
Sbjct: 435 TFNSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELELVLHSLPQIVDAAVM 494

Query: 488 PYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKIL 545
           PYP E+AGQIP+A VV+Q GS + EA+VM  +AK+VAPYKKIR+V FV++IPKSP+GKIL
Sbjct: 495 PYPHEEAGQIPVALVVKQPGSKLTEAEVMYNVAKQVAPYKKIRKVLFVDSIPKSPSGKIL 549

BLAST of Csa1G050300 vs. Swiss-Prot
Match: 4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 1.9e-127
Identity = 244/561 (43.49%), Postives = 362/561 (64.53%), Query Frame = 1

Query: 5   SVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSV 64
           SV+SRSGFC+    + S R P+ LPP +  L V     +   SS       A ID+ +  
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDV-----TTFISSQAHRGRIAFIDASTGQ 62

Query: 65  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 124
           ++++    R + ++   L  +     G V  +LSP S+  PV+   ++SLG +++  NP+
Sbjct: 63  NLTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPL 122

Query: 125 GSESEIAHQVSLCIPAIAFATS-------STASKIPRLPLGTVLIDSPEFLSLMNESNPS 184
            + +EIA Q+    P +AF TS       + A K+P + +    +DS   +  + E    
Sbjct: 123 NTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK 182

Query: 185 DGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVD--GE 244
           +   + + + +++Q+D+A +LYSSGTTG  KGV+ SHRNLI  +       +T+V+  G 
Sbjct: 183 EPSGNRVKE-RVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMV-------QTIVNRFGS 242

Query: 245 IEPHPVALCLLPLFHVFGFFMLFRSI-SEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSP 304
            +     +C +P+FH++G       + + G+T++++ KF+  +M+ A+ KY+ T +P+ P
Sbjct: 243 DDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVP 302

Query: 305 PLIVAMAKS--ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSG 364
           P++VAM     ++ AKYDLSS+  + CGGAPL KEV + F  K+P V+I+QGYGLTES+G
Sbjct: 303 PILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG 362

Query: 365 AAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDE 424
             A T   EE    G+ G+LS +ME +IVDP +G+ L P   GELW++GP IMKGY  +E
Sbjct: 363 IGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNE 422

Query: 425 RATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIL 484
            AT+ TL  EGWL+TGDLCY D DG +++VDRLKELIKYK YQV PAELE LL +HPEI 
Sbjct: 423 EATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEIT 482

Query: 485 DAAVIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSP 544
           DAAVIP+PD++ GQ P+AYVVR+ GS+++E  +M+F+AK+VAPYK+IR+V+FV++IPK+P
Sbjct: 483 DAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNP 542

Query: 545 AGKILRRELVKHALSQGSSKL 554
           +GKILR++L+K A S  +SKL
Sbjct: 543 SGKILRKDLIKIATS--NSKL 546

BLAST of Csa1G050300 vs. TrEMBL
Match: A0A0A0LWL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G050300 PE=4 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV
Sbjct: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480

Query: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540
           DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE
Sbjct: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540

Query: 541 LVKHALSQGSSKL 554
           LVKHALSQGSSKL
Sbjct: 541 LVKHALSQGSSKL 553

BLAST of Csa1G050300 vs. TrEMBL
Match: A0A0A0LR49_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G050290 PE=4 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 3.5e-293
Identity = 517/553 (93.49%), Postives = 530/553 (95.84%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVD RSGFCSQTKIYNSLRPPLSLPPLSQPLT+  H LSVLRSS PPPN+  LIDS
Sbjct: 1   MATHSVDPRSGFCSQTKIYNSLRPPLSLPPLSQPLTIAEHVLSVLRSSSPPPNSAVLIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS +SVSYALFL QIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSGLSVSYALFLHQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEI HQVSLC PAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNES PSDGV
Sbjct: 121 ANPISSESEIVHQVSLCKPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESKPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAIL+SSGTTGR+KGVLISHRNLI+AI G++VLDKT VDGEIE HP
Sbjct: 181 NDGIFDLKINQNDSAAILFSSGTTGRLKGVLISHRNLISAINGVRVLDKTPVDGEIETHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISE +TLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISENHTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480

Query: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540
           DEDAGQIPLAYVVRQ GSNINEAQVMD IAK+VAPYKKIR+VSFVN IPKS  GKILR+E
Sbjct: 481 DEDAGQIPLAYVVRQPGSNINEAQVMDLIAKQVAPYKKIRKVSFVNVIPKSSLGKILRKE 540

Query: 541 LVKHALSQGSSKL 554
           LVKHALSQGS+KL
Sbjct: 541 LVKHALSQGSNKL 553

BLAST of Csa1G050300 vs. TrEMBL
Match: E5GBV5_CUCME (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.1e-243
Identity = 430/456 (94.30%), Postives = 439/456 (96.27%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIY SLRPPLSLPPLSQPLTV GHALSVLRSSPPPPNT ALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYF LLSLGVVVSP
Sbjct: 61  DSGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEIAHQVSLC P IAFA SSTASKIPRLPLGTVLIDSPEFLSLMNESN S+GV
Sbjct: 121 ANPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVL+SHRNLITAITG+QVLDKT VDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYR+TYIPVSPPL++AM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELA KYDLSSLQIL CGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES+GAA+RTVGP
Sbjct: 301 AKSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECS A SVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQ+
Sbjct: 421 PEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQL 456

BLAST of Csa1G050300 vs. TrEMBL
Match: E5GBV5_CUCME (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 1.9e-235
Identity = 418/563 (74.25%), Postives = 476/563 (84.55%), Query Frame = 1

Query: 2    ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61
            A HS+D RSGFC  TKI++SLRPPLSLPPLSQPL+V  HA S+L+SS PPPN+  LIDS+
Sbjct: 497  APHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPNSTLLIDSN 556

Query: 62   SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121
            S + +SYA+FLRQIRNL SNLK+L S  NG VAFIL+PTSLQIPVLYF LLSLGVV+SPA
Sbjct: 557  SGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPA 616

Query: 122  NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181
            NP  S SEI+HQ+ L  P IAFATSSTASK+P L  GTV+IDSP FLS++ E+N SDG+ 
Sbjct: 617  NPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLT 676

Query: 182  DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241
            D    +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +G       + +GE++PHPV
Sbjct: 677  D----IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPV 736

Query: 242  ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301
            ALCLLPLFHVFGF ML R+IS G TLVLM +F+FE MLRAVEK+R+ YIPVSPPL+VAMA
Sbjct: 737  ALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMA 796

Query: 302  KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361
            KS+L AKYDLSSLQIL CGGAPLGKEVIDKFH K P+VEI QGYGLTES+  AART+ PE
Sbjct: 797  KSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPE 856

Query: 362  ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421
            E S   SVGRLS +MEAKIVDP+SGEAL P HKGELW+RGP IMKGYVGD++AT ETL P
Sbjct: 857  EISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDP 916

Query: 422  EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPD 481
            +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPD
Sbjct: 917  DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPD 976

Query: 482  EDAGQIPLAYVVRQLGSNINEAQVMDFIAKK-----------VAPYKKIRRVSFVNAIPK 541
            E+AG+IP+AYVVR+ GSNI+EAQV+DFIAK+           VAPYKKIRRVSF++AIPK
Sbjct: 977  EEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSFIDAIPK 1036

Query: 542  SPAGKILRRELVKHALSQGSSKL 554
            SPAGKILRREL KHALS GSSKL
Sbjct: 1037 SPAGKILRRELAKHALSHGSSKL 1055


HSP 2 Score: 827.8 bits (2137), Expect = 7.9e-237
Identity = 412/550 (74.91%), Postives = 474/550 (86.18%), Query Frame = 1

Query: 4   HSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSS 63
           HS+D RSGFC QTKI++SLRPPLSLPPLS PL+V+ HA S+L+SSPPPPN+  LID +S 
Sbjct: 13  HSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPPPNSTLLIDFNSG 72

Query: 64  VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANP 123
           + +SY +FLRQIRNL SNLK+L S  NGQVAFIL+PTSLQIPVLYF LLSLGVV+SPANP
Sbjct: 73  LHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFALLSLGVVLSPANP 132

Query: 124 IGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDG 183
             S SEI+HQ+ L  P IAFATSSTASK+P+L  GTVLIDSP F S++ E+N SDG+ + 
Sbjct: 133 TSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSMLTETNGSDGIPN- 192

Query: 184 IFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPVAL 243
              +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI    G   L   + +GE+EPHPV L
Sbjct: 193 ---IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGEMEPHPVVL 252

Query: 244 CLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMAKS 303
           C LPLFH+FGF ML R++S G+TLVLM +F+FE MLRAVEKYR+ YIPVSPPL+VAMAKS
Sbjct: 253 CPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPPLVVAMAKS 312

Query: 304 ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPEEC 363
           EL  KYDLSSLQ+L CGGAPLGKEVIDKFH K P VEI+QGYGLTES+  A+RT+ PEE 
Sbjct: 313 ELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGASRTMDPEEI 372

Query: 364 STAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHPEG 423
           S   SVGRLS NMEAKIVDP+SG+AL P HKGELW+RGP IMKGYVGD++AT ETL P+G
Sbjct: 373 SNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKATTETLDPDG 432

Query: 424 WLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPDED 483
           WLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPDE+
Sbjct: 433 WLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPDEE 492

Query: 484 AGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRELVK 543
           AG+IP+AYVVR+ GSN++EAQV+DFIAK+VAPYKKIRRVSF++AIPKSPAGKILRREL K
Sbjct: 493 AGEIPMAYVVRKPGSNMSEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRRELAK 552

Query: 544 HALSQGSSKL 554
           HALS GSSKL
Sbjct: 553 HALSHGSSKL 558

BLAST of Csa1G050300 vs. TrEMBL
Match: M5WU33_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003658mg PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 8.0e-205
Identity = 368/554 (66.43%), Postives = 449/554 (81.05%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVS 65
           +D +SG+C QTK +++LRPP+ LPPLSQPL++   ALS+L+SS   P T  LID+ S   
Sbjct: 11  IDPKSGYCPQTKTFHTLRPPVPLPPLSQPLSITHFALSLLQSSAVAPTTTVLIDAASDHH 70

Query: 66  VSYALFLRQIRNLTSNLKALY-SFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 125
           VSY  F+ Q+ +LT +L++L  + S G VAFILSPTSL +PVLYF LL+LGVVVSPANPI
Sbjct: 71  VSYENFIGQVHSLTLSLRSLAPALSKGHVAFILSPTSLHVPVLYFSLLALGVVVSPANPI 130

Query: 126 GSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTV-LIDSPEFLSLMNESNPSDGVNDG 185
           GS+SEIA+QV L  PAIAFATS+TA K+P+  L T+ L+DSPEFLS++N    SD   D 
Sbjct: 131 GSDSEIAYQVRLTRPAIAFATSATAHKLPKDELRTIILLDSPEFLSMLNRVGSSDTRPD- 190

Query: 186 IFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVL----DKTLVDGEIEPH 245
            F ++++Q DSAAIL+SSGTTGRVKGV+++HRN I  + GL  L    D TL + ++   
Sbjct: 191 -FGIEVSQTDSAAILFSSGTTGRVKGVILTHRNFIGLLAGLHTLRREPDLTLPEEQV--- 250

Query: 246 PVALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVA 305
            V+L  LPLFHVFGFFML R+++ G TLVLM +FDFE MLRAVE+Y+++Y+PVSPPLIVA
Sbjct: 251 -VSLFTLPLFHVFGFFMLVRAVAMGETLVLMERFDFEAMLRAVERYKVSYMPVSPPLIVA 310

Query: 306 MAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVG 365
           + KSELA KYDLSSL++L CGGAPLGKEV ++F  +FPNVE++QGYGLTE+  AA R + 
Sbjct: 311 LVKSELAQKYDLSSLRLLGCGGAPLGKEVAERFTERFPNVELVQGYGLTETGAAATRMID 370

Query: 366 PEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETL 425
            EE     SVGRL+ENMEA+IVDP +GEALPPG +GELW+RGP +MKGYVGD++ATAETL
Sbjct: 371 SEESKRYASVGRLAENMEAQIVDPETGEALPPGRRGELWLRGPTVMKGYVGDDKATAETL 430

Query: 426 HPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPY 485
              GWLKTGDLC+FD +G LYIVDRLKELIKYKAYQVPPAELE +LQSHP+I DAAVIPY
Sbjct: 431 DSAGWLKTGDLCFFDDEGFLYIVDRLKELIKYKAYQVPPAELEPILQSHPDIADAAVIPY 490

Query: 486 PDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRR 545
           PDE+AGQIP+AYVVR+ GSNI EA VMDF+AK+VAPYKKIRRVSF+N+IPKSPAGKILRR
Sbjct: 491 PDEEAGQIPMAYVVRKAGSNITEAAVMDFVAKQVAPYKKIRRVSFINSIPKSPAGKILRR 550

Query: 546 ELVKHALSQGSSKL 554
           ELV HALS  SSKL
Sbjct: 551 ELVSHALSSRSSKL 558

BLAST of Csa1G050300 vs. TAIR10
Match: AT5G63380.1 (AT5G63380.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 664.1 bits (1712), Expect = 7.7e-191
Identity = 341/558 (61.11%), Postives = 429/558 (76.88%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPA-------LI 65
           +D  SGF  +T IY+SLRP LSLPP+ QPL+    ALS+L  S PP            L+
Sbjct: 11  IDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEALTYLV 70

Query: 66  DSDSSVSVSYALFLRQIRNLTSNLKALY-SFSNGQVAFILSPTSLQIPVLYFVLLSLGVV 125
           +S S  +++Y   LR++R+L  +L+  + S ++  VAFILSP+SL IPVLY  L+S+GVV
Sbjct: 71  NSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV 130

Query: 126 VSPANPIGSESEIAHQVSLCIPAIAFATSSTASKIPR--LPLGTVLIDSPEFLSLMNESN 185
           VSPANPIGSESE++HQV +  P IAFATS T  K+    LPLGTVL+DS EFLS +N S+
Sbjct: 131 VSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSD 190

Query: 186 PSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGE 245
            S  VN   F +++NQ+D AAIL+SSGTTGRVKGVL++HRNLI +     V  +  +   
Sbjct: 191 -SSSVNP--FQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTA---VSHQRTLQDP 250

Query: 246 IEPHPVALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPP 305
           +    V L  LPLFHVFGF M+ R+IS G TLVL+ +F+ E M +AVEKY++T +PVSPP
Sbjct: 251 VNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVTGMPVSPP 310

Query: 306 LIVAMAKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAA 365
           LIVA+ KSEL  KYDL SL+ L CGGAPLGK++ ++F  KFP+V+I+QGYGLTESSG AA
Sbjct: 311 LIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLTESSGPAA 370

Query: 366 RTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERAT 425
            T GPEE    GSVGR+SENMEAKIVDPS+GE+LPPG  GELW+RGP IMKGYVG+E+A+
Sbjct: 371 STFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKAS 430

Query: 426 AETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAA 485
           AET+  EGWLKTGDLCYFDS+  LYIVDRLKELIKYKAYQVPP ELE +L S+P+++DAA
Sbjct: 431 AETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAA 490

Query: 486 VIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGK 545
           V+P+PDEDAG+IP+A++VR+ GSN+NEAQ++DF+AK+V PYKK+RRV+F+NAIPK+PAGK
Sbjct: 491 VVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAIPKNPAGK 550

Query: 546 ILRRELVKHALSQGSSKL 554
           ILRREL K A+   +SKL
Sbjct: 551 ILRRELTKIAVDGNASKL 562

BLAST of Csa1G050300 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.1e-128
Identity = 244/561 (43.49%), Postives = 362/561 (64.53%), Query Frame = 1

Query: 5   SVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSV 64
           SV+SRSGFC+    + S R P+ LPP +  L V     +   SS       A ID+ +  
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDV-----TTFISSQAHRGRIAFIDASTGQ 62

Query: 65  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 124
           ++++    R + ++   L  +     G V  +LSP S+  PV+   ++SLG +++  NP+
Sbjct: 63  NLTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPL 122

Query: 125 GSESEIAHQVSLCIPAIAFATS-------STASKIPRLPLGTVLIDSPEFLSLMNESNPS 184
            + +EIA Q+    P +AF TS       + A K+P + +    +DS   +  + E    
Sbjct: 123 NTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKK 182

Query: 185 DGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVD--GE 244
           +   + + + +++Q+D+A +LYSSGTTG  KGV+ SHRNLI  +       +T+V+  G 
Sbjct: 183 EPSGNRVKE-RVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMV-------QTIVNRFGS 242

Query: 245 IEPHPVALCLLPLFHVFGFFMLFRSI-SEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSP 304
            +     +C +P+FH++G       + + G+T++++ KF+  +M+ A+ KY+ T +P+ P
Sbjct: 243 DDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVP 302

Query: 305 PLIVAMAKS--ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSG 364
           P++VAM     ++ AKYDLSS+  + CGGAPL KEV + F  K+P V+I+QGYGLTES+G
Sbjct: 303 PILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG 362

Query: 365 AAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDE 424
             A T   EE    G+ G+LS +ME +IVDP +G+ L P   GELW++GP IMKGY  +E
Sbjct: 363 IGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNE 422

Query: 425 RATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIL 484
            AT+ TL  EGWL+TGDLCY D DG +++VDRLKELIKYK YQV PAELE LL +HPEI 
Sbjct: 423 EATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEIT 482

Query: 485 DAAVIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSP 544
           DAAVIP+PD++ GQ P+AYVVR+ GS+++E  +M+F+AK+VAPYK+IR+V+FV++IPK+P
Sbjct: 483 DAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNP 542

Query: 545 AGKILRRELVKHALSQGSSKL 554
           +GKILR++L+K A S  +SKL
Sbjct: 543 SGKILRKDLIKIATS--NSKL 546

BLAST of Csa1G050300 vs. TAIR10
Match: AT1G20500.1 (AT1G20500.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 455.7 bits (1171), Expect = 4.1e-128
Identity = 246/556 (44.24%), Postives = 351/556 (63.13%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPP-LSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSV 65
           VD RSGFC     + S R PLSLPP LS+ +T          SS P     A ID+ +  
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTT-------FISSQPHRGKTAFIDAATGQ 69

Query: 66  SVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPI 125
            ++++   R +  +   L        G V  ILSP S+ IPV+   ++SLG V + AN +
Sbjct: 70  CLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTL 129

Query: 126 GSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEF---------LSLMNESN 185
            +  EI+ Q++   P + F T   A K+P + +  VL D   +         + +++E  
Sbjct: 130 NTSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMV 189

Query: 186 PSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGE 245
             +     + D ++NQ+D+A +LYSSGTTG  KGV+ SHRNL   +          +   
Sbjct: 190 KKEPSGQRVRD-RVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVA-------RFISDN 249

Query: 246 IEPHPVALCLLPLFHVFGFFML-FRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSP 305
           ++   + +C +P+FH +G       +++ G+T+V++R+F    M+ AVEK+R T + ++P
Sbjct: 250 LKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAP 309

Query: 306 PLIVAMAKSE--LAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSG 365
           P++VAM      + AKYDLSSL+ + CGGAPL KEV + F  K+P V+I+QGY LTES+G
Sbjct: 310 PVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNG 369

Query: 366 AAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDE 425
             A T   EE    G+ G L+ ++EA+IVDP++G  +     GELW++GP I KGY  ++
Sbjct: 370 GGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQ 429

Query: 426 RATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIL 485
            AT ET++ EGWLKTGDLCY D DG L++VDRLKELIKYK YQVPPAELE LL +HP+IL
Sbjct: 430 EATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDIL 489

Query: 486 DAAVIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSP 545
           DAAVIP+PD++AGQ P+AYVVR+  SN++E QV+DFI+K+VAPYKKIR+VSF+N+IPK+ 
Sbjct: 490 DAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTA 549

Query: 546 AGKILRRELVKHALSQ 549
           +GK LR++L+K A S+
Sbjct: 550 SGKTLRKDLIKLATSK 549

BLAST of Csa1G050300 vs. TAIR10
Match: AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-123
Identity = 248/566 (43.82%), Postives = 342/566 (60.42%), Query Frame = 1

Query: 3   THSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDS 62
           T +VD +SGFC  T I+ S R P++LPP +Q L V         +S P       +D+ +
Sbjct: 18  TLAVDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVT 77

Query: 63  SVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPAN 122
              +S+      +  +   L AL     G V  ILSP S+  P++   ++SLG +++ AN
Sbjct: 78  GRRLSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTAN 137

Query: 123 PIGSESEIAHQVSLCIPAIAFATSSTASKIP---RLPLGTVLIDSPEF------------ 182
           PI +  EI+ Q+    P +AF T    SK+       L  VL+D                
Sbjct: 138 PINTSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLV 197

Query: 183 --LSLMNESNPSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQ 242
             L  M E+ PS+         ++NQ+D+AA+LYSSGTTG  KGV++SHRNLI  +   +
Sbjct: 198 GRLETMIETEPSESR----VKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYR 257

Query: 243 VLDKTLVDGEIEPHPVALCLLPLFHVFGFFMLFRS-ISEGNTLVLMRKFDFEKMLRAVEK 302
                            +C +P+ H+FGF       I+ G T+V++ KFD  K+L AVE 
Sbjct: 258 A--------RFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVET 317

Query: 303 YRITYIPVSPPLIVAMAK--SELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEII 362
           +R +Y+ + PP++VAM    +E+ +KYDLSSL  +  GGAPL +EV +KF   +P V+I+
Sbjct: 318 HRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKIL 377

Query: 363 QGYGLTESSGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGP 422
           QGYGLTES+  AA     EE    G+ G L+ N+E KIVDP +G  L     GELWIR P
Sbjct: 378 QGYGLTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSP 437

Query: 423 GIMKGYVGDERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELE 482
            +MKGY  ++ ATA T+  EGWLKTGDLCY D DG +++VDRLKELIK   YQV PAELE
Sbjct: 438 TVMKGYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELE 497

Query: 483 HLLQSHPEILDAAVIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRV 542
            LL +HPEI DAAVIP PD  AGQ P+AY+VR++GSN++E+++M F+AK+V+PYKKIR+V
Sbjct: 498 ALLLAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKV 557

Query: 543 SFVNAIPKSPAGKILRRELVKHALSQ 549
           +F+ +IPK+P+GKILRREL K   S+
Sbjct: 558 TFLASIPKNPSGKILRRELTKLTTSK 564

BLAST of Csa1G050300 vs. TAIR10
Match: AT5G38120.1 (AT5G38120.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 427.2 bits (1097), Expect = 1.6e-119
Identity = 235/561 (41.89%), Postives = 343/561 (61.14%), Query Frame = 1

Query: 6   VDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSSVS 65
           +D R+GFC+    + S R PL+LP   + L +     +   SS       A ID+ +   
Sbjct: 11  IDPRNGFCTSNSTFYSKRKPLALPS-KESLDI-----TTFISSQTYRGKTAFIDAATDHR 70

Query: 66  VSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANPIG 125
           +S++     +  +   L        G V  +LSP ++ IP++   ++SLG V++ ANP+ 
Sbjct: 71  ISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLN 130

Query: 126 SESEIAHQVSLCIPAIAFATSSTASKIP---------------RLPLGTVLIDSPEFLSL 185
           + SEI  Q++   P +AF T   A KI                R+P G  ++ +     +
Sbjct: 131 TASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVGN--LTEM 190

Query: 186 MNESNPSDGVNDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKT 245
           M +      V +     +++++D+A +LYSSGTTGR KGV  SH NLI  +       + 
Sbjct: 191 MKKEPSGQAVRN-----QVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVA------RY 250

Query: 246 LVDGEIEPHPVALCLLPLFHVFGFF-MLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITY 305
           + +   +P    +C +PLFH FG    +  +++ G T+V++ +FD  +M+ AVEKYR T 
Sbjct: 251 IAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATT 310

Query: 306 IPVSPPLIVAMAKS--ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGL 365
           + + PP++V M     ++  KYD+S L+ + CGGAPL KEV   F  K+P V++ QGY L
Sbjct: 311 LILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYAL 370

Query: 366 TESSGAAARTVGPEECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKG 425
           TES+GA A     EE    G+VG LS  +EA+IVDP++G+ +     GELW++GP I KG
Sbjct: 371 TESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKG 430

Query: 426 YVGDERATAETLHPEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQS 485
           Y  +E    E +  EGWLKTGDLCY D+DG L+IVDRLKELIKYK YQVPPAELE LL +
Sbjct: 431 YFRNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLN 490

Query: 486 HPEILDAAVIPYPDEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNA 545
           HP+ILDAAVIP+PD++AGQ P+AYV R+  SN+ E +V+DFI+K+VAPYKKIR+V+F+++
Sbjct: 491 HPDILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDS 549

Query: 546 IPKSPAGKILRRELVKHALSQ 549
           IPK+P+GK LR++L+K A+S+
Sbjct: 551 IPKTPSGKTLRKDLIKFAISK 549

BLAST of Csa1G050300 vs. NCBI nr
Match: gi|778658041|ref|XP_004152591.2| (PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis sativus])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV
Sbjct: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480

Query: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540
           DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE
Sbjct: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540

Query: 541 LVKHALSQGSSKL 554
           LVKHALSQGSSKL
Sbjct: 541 LVKHALSQGSSKL 553

BLAST of Csa1G050300 vs. NCBI nr
Match: gi|659068783|ref|XP_008446209.1| (PREDICTED: uncharacterized protein LOC103489008 [Cucumis melo])

HSP 1 Score: 1023.8 bits (2646), Expect = 1.1e-295
Identity = 518/552 (93.84%), Postives = 532/552 (96.38%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIY SLRPPLSLPPLSQPLTV GHALSVLRSSPPPPNT ALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYF LLSLGVVVSP
Sbjct: 61  DSGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEIAHQVSLC P IAFA SSTASKIPRLPLGTVLIDSPEFLSLMNESN S+GV
Sbjct: 121 ANPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVL+SHRNLITAITG+QVLDKT VDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYR+TYIPVSPPL++AM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELA KYDLSSLQIL CGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES+GAA+RTVGP
Sbjct: 301 AKSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECS A SVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480
           PEGWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP
Sbjct: 421 PEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480

Query: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540
           DE+AGQIP+AYVVRQ GSNINE QVMDFIAK+VAPYKKIRRV FVNA+PKSPAGKILRRE
Sbjct: 481 DEEAGQIPVAYVVRQPGSNINETQVMDFIAKQVAPYKKIRRVFFVNAVPKSPAGKILRRE 540

Query: 541 LVKHALSQGSSK 553
           LV+HALSQGSS+
Sbjct: 541 LVQHALSQGSSR 552

BLAST of Csa1G050300 vs. NCBI nr
Match: gi|659068783|ref|XP_008446209.1| (PREDICTED: uncharacterized protein LOC103489008 [Cucumis melo])

HSP 1 Score: 831.6 bits (2147), Expect = 7.9e-238
Identity = 418/552 (75.72%), Postives = 476/552 (86.23%), Query Frame = 1

Query: 2    ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61
            A HS+D RSGFC  TKI++SLRPPLSLPPLSQPL+V  HA S+L+SS PPPN+  LIDS+
Sbjct: 576  APHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPNSTLLIDSN 635

Query: 62   SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121
            S + +SYA+FLRQIRNL SNLK+L S  NG VAFIL+PTSLQIPVLYF LLSLGVV+SPA
Sbjct: 636  SGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPA 695

Query: 122  NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181
            NP  S SEI+HQ+ L  P IAFATSSTASK+P L  GTV+IDSP FLS++ E+N SDG+ 
Sbjct: 696  NPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLT 755

Query: 182  DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241
            D    +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +G       + +GE++PHPV
Sbjct: 756  D----IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPV 815

Query: 242  ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301
            ALCLLPLFHVFGF ML R+IS G TLVLM +F+FE MLRAVEK+R+ YIPVSPPL+VAMA
Sbjct: 816  ALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMA 875

Query: 302  KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361
            KS+L AKYDLSSLQIL CGGAPLGKEVIDKFH K P+VEI QGYGLTES+  AART+ PE
Sbjct: 876  KSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPE 935

Query: 362  ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421
            E S   SVGRLS +MEAKIVDP+SGEAL P HKGELW+RGP IMKGYVGD++AT ETL P
Sbjct: 936  EISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDP 995

Query: 422  EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPD 481
            +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPD
Sbjct: 996  DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPD 1055

Query: 482  EDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRREL 541
            E+AG+IP+AYVVR+ GSNI+EAQV+DFIAK+VAPYKKIRRVSF++AIPKSPAGKILRREL
Sbjct: 1056 EEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRREL 1115

Query: 542  VKHALSQGSSKL 554
             KHALS GSSKL
Sbjct: 1116 AKHALSHGSSKL 1123


HSP 2 Score: 1015.0 bits (2623), Expect = 5.0e-293
Identity = 517/553 (93.49%), Postives = 530/553 (95.84%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVD RSGFCSQTKIYNSLRPPLSLPPLSQPLT+  H LSVLRSS PPPN+  LIDS
Sbjct: 1   MATHSVDPRSGFCSQTKIYNSLRPPLSLPPLSQPLTIAEHVLSVLRSSSPPPNSAVLIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS +SVSYALFL QIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP
Sbjct: 61  DSGLSVSYALFLHQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEI HQVSLC PAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNES PSDGV
Sbjct: 121 ANPISSESEIVHQVSLCKPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESKPSDGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAIL+SSGTTGR+KGVLISHRNLI+AI G++VLDKT VDGEIE HP
Sbjct: 181 NDGIFDLKINQNDSAAILFSSGTTGRLKGVLISHRNLISAINGVRVLDKTPVDGEIETHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISE +TLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISENHTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP
Sbjct: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480
           PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP
Sbjct: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYP 480

Query: 481 DEDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRE 540
           DEDAGQIPLAYVVRQ GSNINEAQVMD IAK+VAPYKKIR+VSFVN IPKS  GKILR+E
Sbjct: 481 DEDAGQIPLAYVVRQPGSNINEAQVMDLIAKQVAPYKKIRKVSFVNVIPKSSLGKILRKE 540

Query: 541 LVKHALSQGSSKL 554
           LVKHALSQGS+KL
Sbjct: 541 LVKHALSQGSNKL 553

BLAST of Csa1G050300 vs. NCBI nr
Match: gi|307136108|gb|ADN33954.1| (4-coumarate-CoA ligase [Cucumis melo subsp. melo])

HSP 1 Score: 850.5 bits (2196), Expect = 1.6e-243
Identity = 430/456 (94.30%), Postives = 439/456 (96.27%), Query Frame = 1

Query: 1   MATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDS 60
           MATHSVDSRSGFCSQTKIY SLRPPLSLPPLSQPLTV GHALSVLRSSPPPPNT ALIDS
Sbjct: 1   MATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDS 60

Query: 61  DSSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSP 120
           DS VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYF LLSLGVVVSP
Sbjct: 61  DSGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSP 120

Query: 121 ANPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGV 180
           ANPI SESEIAHQVSLC P IAFA SSTASKIPRLPLGTVLIDSPEFLSLMNESN S+GV
Sbjct: 121 ANPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGV 180

Query: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHP 240
           NDGIFDLKINQNDSAAILYSSGTTGRVKGVL+SHRNLITAITG+QVLDKT VDGEIEPHP
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHP 240

Query: 241 VALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAM 300
           VAL LLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYR+TYIPVSPPL++AM
Sbjct: 241 VALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAM 300

Query: 301 AKSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGP 360
           AKSELA KYDLSSLQIL CGGAPLGKEVIDKFHVKFPNVEIIQGYGLTES+GAA+RTVGP
Sbjct: 301 AKSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGP 360

Query: 361 EECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420
           EECS A SVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH
Sbjct: 361 EECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLH 420

Query: 421 PEGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQV 457
           PEGWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQ+
Sbjct: 421 PEGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQL 456

BLAST of Csa1G050300 vs. NCBI nr
Match: gi|307136108|gb|ADN33954.1| (4-coumarate-CoA ligase [Cucumis melo subsp. melo])

HSP 1 Score: 823.2 bits (2125), Expect = 2.8e-235
Identity = 418/563 (74.25%), Postives = 476/563 (84.55%), Query Frame = 1

Query: 2    ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61
            A HS+D RSGFC  TKI++SLRPPLSLPPLSQPL+V  HA S+L+SS PPPN+  LIDS+
Sbjct: 497  APHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPPPNSTLLIDSN 556

Query: 62   SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121
            S + +SYA+FLRQIRNL SNLK+L S  NG VAFIL+PTSLQIPVLYF LLSLGVV+SPA
Sbjct: 557  SGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFALLSLGVVLSPA 616

Query: 122  NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181
            NP  S SEI+HQ+ L  P IAFATSSTASK+P L  GTV+IDSP FLS++ E+N SDG+ 
Sbjct: 617  NPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSMLTETNGSDGLT 676

Query: 182  DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241
            D    +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +G       + +GE++PHPV
Sbjct: 677  D----IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGEMKPHPV 736

Query: 242  ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301
            ALCLLPLFHVFGF ML R+IS G TLVLM +F+FE MLRAVEK+R+ YIPVSPPL+VAMA
Sbjct: 737  ALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPPLVVAMA 796

Query: 302  KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361
            KS+L AKYDLSSLQIL CGGAPLGKEVIDKFH K P+VEI QGYGLTES+  AART+ PE
Sbjct: 797  KSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAARTMEPE 856

Query: 362  ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421
            E S   SVGRLS +MEAKIVDP+SGEAL P HKGELW+RGP IMKGYVGD++AT ETL P
Sbjct: 857  EISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKATTETLDP 916

Query: 422  EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPD 481
            +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPD
Sbjct: 917  DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPD 976

Query: 482  EDAGQIPLAYVVRQLGSNINEAQVMDFIAKK-----------VAPYKKIRRVSFVNAIPK 541
            E+AG+IP+AYVVR+ GSNI+EAQV+DFIAK+           VAPYKKIRRVSF++AIPK
Sbjct: 977  EEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSFIDAIPK 1036

Query: 542  SPAGKILRRELVKHALSQGSSKL 554
            SPAGKILRREL KHALS GSSKL
Sbjct: 1037 SPAGKILRRELAKHALSHGSSKL 1055


HSP 2 Score: 827.8 bits (2137), Expect = 1.1e-236
Identity = 412/550 (74.91%), Postives = 474/550 (86.18%), Query Frame = 1

Query: 4   HSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSDSS 63
           HS+D RSGFC QTKI++SLRPPLSLPPLS PL+V+ HA S+L+SSPPPPN+  LID +S 
Sbjct: 13  HSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPPPNSTLLIDFNSG 72

Query: 64  VSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPANP 123
           + +SY +FLRQIRNL SNLK+L S  NGQVAFIL+PTSLQIPVLYF LLSLGVV+SPANP
Sbjct: 73  LHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFALLSLGVVLSPANP 132

Query: 124 IGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVNDG 183
             S SEI+HQ+ L  P IAFATSSTASK+P+L  GTVLIDSP F S++ E+N SDG+ + 
Sbjct: 133 TSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSMLTETNGSDGIPN- 192

Query: 184 IFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPVAL 243
              +KI+Q+DSAAILYSSGTTGRVKGVL+SHRNLI    G   L   + +GE+EPHPV L
Sbjct: 193 ---IKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGEMEPHPVVL 252

Query: 244 CLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMAKS 303
           C LPLFH+FGF ML R++S G+TLVLM +F+FE MLRAVEKYR+ YIPVSPPL+VAMAKS
Sbjct: 253 CPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPPLVVAMAKS 312

Query: 304 ELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPEEC 363
           EL  KYDLSSLQ+L CGGAPLGKEVIDKFH K P VEI+QGYGLTES+  A+RT+ PEE 
Sbjct: 313 ELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGASRTMDPEEI 372

Query: 364 STAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHPEG 423
           S   SVGRLS NMEAKIVDP+SG+AL P HKGELW+RGP IMKGYVGD++AT ETL P+G
Sbjct: 373 SNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKATTETLDPDG 432

Query: 424 WLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPDED 483
           WLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPDE+
Sbjct: 433 WLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPDEE 492

Query: 484 AGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRRELVK 543
           AG+IP+AYVVR+ GSN++EAQV+DFIAK+VAPYKKIRRVSF++AIPKSPAGKILRREL K
Sbjct: 493 AGEIPMAYVVRKPGSNMSEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRRELAK 552

Query: 544 HALSQGSSKL 554
           HALS GSSKL
Sbjct: 553 HALSHGSSKL 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
4CLL9_ARATH1.4e-18961.114-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana GN=4CLL9 PE=1 SV=2[more]
4CLL5_ORYSJ1.6e-16155.044-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica GN=4CLL5 PE=2 SV=... [more]
4CLL6_ORYSJ3.9e-16053.754-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica GN=4CLL6 PE=2 SV=... [more]
4CLL7_ORYSJ6.6e-13646.984-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica GN=4CLL7 PE=2 SV=... [more]
4CLL5_ARATH1.9e-12743.494-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana GN=4CLL5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LWL5_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G050300 PE=4 SV=1[more]
A0A0A0LR49_CUCSA3.5e-29393.49Uncharacterized protein OS=Cucumis sativus GN=Csa_1G050290 PE=4 SV=1[more]
E5GBV5_CUCME1.1e-24394.304-coumarate-CoA ligase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
E5GBV5_CUCME1.9e-23574.254-coumarate-CoA ligase OS=Cucumis melo subsp. melo PE=4 SV=1[more]
M5WU33_PRUPE8.0e-20566.43Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003658mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G63380.17.7e-19161.11 AMP-dependent synthetase and ligase family protein[more]
AT1G20510.11.1e-12843.49 OPC-8:0 CoA ligase1[more]
AT1G20500.14.1e-12844.24 AMP-dependent synthetase and ligase family protein[more]
AT1G20480.11.1e-12343.82 AMP-dependent synthetase and ligase family protein[more]
AT5G38120.11.6e-11941.89 AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
gi|778658041|ref|XP_004152591.2|0.0e+00100.00PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis sativus][more]
gi|659068783|ref|XP_008446209.1|1.1e-29593.84PREDICTED: uncharacterized protein LOC103489008 [Cucumis melo][more]
gi|659068783|ref|XP_008446209.1|7.9e-23875.72PREDICTED: uncharacterized protein LOC103489008 [Cucumis melo][more]
gi|307136108|gb|ADN33954.1|1.6e-24394.304-coumarate-CoA ligase [Cucumis melo subsp. melo][more]
gi|307136108|gb|ADN33954.1|2.8e-23574.254-coumarate-CoA ligase [Cucumis melo subsp. melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016874 ligase activity
molecular_function GO:0003824 catalytic activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU092239cucumber EST collection version 3.0transcribed_cluster
CU163298cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa1G050300.2Csa1G050300.2mRNA
Csa1G050300.1Csa1G050300.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU092239CU092239transcribed_cluster
CU163298CU163298transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 52..451
score: 9.4
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 197..208
scor
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 367..442
score: 3.4
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 52..202
score: 3.3E-18coord: 203..357
score: 7.5
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 1..456
score: 1.3E
NoneNo IPR availablePANTHERPTHR24096:SF2514-COUMARATE--COA LIGASE-LIKE 9coord: 1..456
score: 1.3E
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 51..456
score: 1.57

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa1G050300Cla97C03G054710Watermelon (97103) v2cuwmbB032
The following gene(s) are paralogous to this gene:

None