CsGy4G013150.1 (mRNA) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy4G013150.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	linoleate 13S-lipoxygenase 2-1, chloroplastic-like
Location	Chr4 : 18303607 .. 18304315 (+)
Sequence length	618

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAAACTCTTCAATACATTATAGGTCAAACCAAGCTTCGTTTGCGTATCAAAAGTCATTTATTCATAGAAATGTTGAACCTGTATTAGTCCATCTACATCAAGGGTTAAAACCAAATCCTTCTCATATTGGTTTCACCAATAATCTCAGTGTTGTTAAAGAGTCATCCTTTGCTATGACTTCCATCAACAGGCCAATTTCAATCAAAGTCGTGGTTACAATCAAACAGTGCATCAATGATTTGTTTCCACATTTTGGGTTCAAACCAAGGCTCGACCATATCACTAATTGGTGGCCAAGTCAAGCTCTTGGTTTGGAGTTTATTAGCAATGAGATGGATCCAAGTATGTTAATTTACTTAGCTTTATATATCAATGTTTTTATATTTAAGACATAGATAATTTAGACTTCATATATATATAATGTATTGATCAGCAACAGGATTGGAGAAGGGAACAACAAAAGCTTATGCAGAGAGAGTGAAGAGAAAGGATAACGAGGTAATTGTGTATGAGACAAAGGTGGTAATTCCAGCAGATTTTGGTGCAATTGGAGGAGTTTTGGTTGAGAATGAACACAACAAAGAGATGTTTCTTATGGACATTGTGATTCATGGAATTCCAACACAATATCATCTTCATTTCCCATGCAATTCTTGGATTCAGAGCAAAGATAGAAGAATCTTCTTCACCACTAAGGTCATTTAA

mRNA sequence

ATGCTAAACTCTTCAATACATTATAGGTCAAACCAAGCTTCGTTTGCGTATCAAAAGTCATTTATTCATAGAAATGTTGAACCTGTATTAGTCCATCTACATCAAGGGTTAAAACCAAATCCTTCTCATATTGGTTTCACCAATAATCTCAGTGTTGTTAAAGAGTCATCCTTTGCTATGACTTCCATCAACAGGCCAATTTCAATCAAAGTCGTGGTTACAATCAAACAGTGCATCAATGATTTGTTTCCACATTTTGGGTTCAAACCAAGGCTCGACCATATCACTAATTGGTGGCCAAGTCAAGCTCTTGGTTTGGAGTTTATTAGCAATGAGATGGATCCAACAACAGGATTGGAGAAGGGAACAACAAAAGCTTATGCAGAGAGAGTGAAGAGAAAGGATAACGAGGTAATTGTGTATGAGACAAAGGTGGTAATTCCAGCAGATTTTGGTGCAATTGGAGGAGTTTTGGTTGAGAATGAACACAACAAAGAGATGTTTCTTATGGACATTGTGATTCATGGAATTCCAACACAATATCATCTTCATTTCCCATGCAATTCTTGGATTCAGAGCAAAGATAGAAGAATCTTCTTCACCACTAAGGTCATTTAA

Coding sequence (CDS)

ATGCTAAACTCTTCAATACATTATAGGTCAAACCAAGCTTCGTTTGCGTATCAAAAGTCATTTATTCATAGAAATGTTGAACCTGTATTAGTCCATCTACATCAAGGGTTAAAACCAAATCCTTCTCATATTGGTTTCACCAATAATCTCAGTGTTGTTAAAGAGTCATCCTTTGCTATGACTTCCATCAACAGGCCAATTTCAATCAAAGTCGTGGTTACAATCAAACAGTGCATCAATGATTTGTTTCCACATTTTGGGTTCAAACCAAGGCTCGACCATATCACTAATTGGTGGCCAAGTCAAGCTCTTGGTTTGGAGTTTATTAGCAATGAGATGGATCCAACAACAGGATTGGAGAAGGGAACAACAAAAGCTTATGCAGAGAGAGTGAAGAGAAAGGATAACGAGGTAATTGTGTATGAGACAAAGGTGGTAATTCCAGCAGATTTTGGTGCAATTGGAGGAGTTTTGGTTGAGAATGAACACAACAAAGAGATGTTTCTTATGGACATTGTGATTCATGGAATTCCAACACAATATCATCTTCATTTCCCATGCAATTCTTGGATTCAGAGCAAAGATAGAAGAATCTTCTTCACCACTAAGGTCATTTAA

Protein sequence

MLNSSIHYRSNQASFAYQKSFIHRNVEPVLVHLHQGLKPNPSHIGFTNNLSVVKESSFAMTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPTQYHLHFPCNSWIQSKDRRIFFTTKVI

Homology

[Close]

BLAST of CsGy4G013150.1 vs. NCBI nr
Match: XP_016900734.1 (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 374.0 bits (959), Expect = 3.2e-100
Identity = 181/205 (88.29%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MLNSSIHYRSNQASFAYQKSFIHRNVEPVLVHLHQGLKPNPSHIGFTNNLSVVKESSFAM 60
           MLNSSI YRSNQASFAYQK FIHRN E + +HL QG KPN S IG TNNLSVVKESSFAM
Sbjct: 1   MLNSSILYRSNQASFAYQKPFIHRNFEVLSLHLDQGFKPNASRIGSTNNLSVVKESSFAM 60

Query: 61  TSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGLE 120
           TSINRPIS+KVVVTIK+CIN+LFPHFGFKPRLDHITNWW SQAL LEFIS EMDPTTGLE
Sbjct: 61  TSINRPISVKVVVTIKRCINELFPHFGFKPRLDHITNWWTSQALRLEFISVEMDPTTGLE 120

Query: 121 KGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPTQ 180
           KGTTKAYAE+VKR+DNEVIVYETKVV+PADFGAIGGVLVENE NKE+FLMDIVIHGIPTQ
Sbjct: 121 KGTTKAYAEKVKREDNEVIVYETKVVLPADFGAIGGVLVENERNKELFLMDIVIHGIPTQ 180

Query: 181 YHLHFPCNSWIQSKDRRIFFTTKVI 206
           YHLHFPCNSWIQSKD+RIFFTTKVI
Sbjct: 181 YHLHFPCNSWIQSKDKRIFFTTKVI 205

[Close]

BLAST of CsGy4G013150.1 vs. NCBI nr
Match: KGN54139.1 (hypothetical protein Csa_4G288100 [Cucumis sativus])

HSP 1 Score: 308.9 bits (790), Expect = 1.3e-80
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 60  MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL 119
           MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL
Sbjct: 1   MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL 60

Query: 120 EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT 179
           EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT
Sbjct: 61  EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT 120

Query: 180 QYHLHFPCNSWIQSKDRRIFFTTKVI 206
           QYHLHFPCNSWIQSKDRRIFFTTKVI
Sbjct: 121 QYHLHFPCNSWIQSKDRRIFFTTKVI 146

[Close]

BLAST of CsGy4G013150.1 vs. NCBI nr
Match: XP_004142235.1 (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-79
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 60  MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL 119
           MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL
Sbjct: 1   MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL 60

Query: 120 EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT 179
           EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT
Sbjct: 61  EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT 120

Query: 180 QYHLHFPCNSWIQSKDRRIFFTTK 204
           QYHLHFPCNSWIQSKDRRIFFTTK
Sbjct: 121 QYHLHFPCNSWIQSKDRRIFFTTK 144

[Close]

BLAST of CsGy4G013150.1 vs. NCBI nr
Match: XP_022958530.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 181.8 bits (460), Expect = 2.4e-42
Identity = 115/213 (53.99%), Postives = 141/213 (66.20%), Query Frame = 0


Query: 1   MLN-SSIHYR-SNQAS-FAYQKSFIHRNVEPVLVHLHQGLKPNPSHIGFTNNLSVVKESS 60
           MLN   +HYR SNQA+ F YQK  +  NV+  +   H    P  S + FT+  +V+K S+
Sbjct: 1   MLNHPPLHYRCSNQATWFLYQKPLLPGNVDACVSLQHSLTPPAASPLRFTSP-NVIKPSA 60

Query: 61  --FAMTSINRPISIK-VVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMD 120
              ++ SI  P+  K VV T+++ I+ LFP FGF  RL  I N   S+ L LE +S +MD
Sbjct: 61  VHVSVPSIQSPVYTKAVVTTVQRPIHQLFPKFGFIGRLHDIIN-RRSRVLALELLSADMD 120

Query: 121 PTTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVI 180
           PTTGLEKG  KAY ++VKR+D+E I+YE   VIPADFG IG VLVEN H KE+FL DIVI
Sbjct: 121 PTTGLEKGRMKAYEQKVKREDDE-IIYEISFVIPADFGPIGAVLVENMHKKEVFLKDIVI 180

Query: 181 HGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           HGIPT   LHF CNSWI SK    DRRIFFTTK
Sbjct: 181 HGIPTG-PLHFSCNSWITSKAQCNDRRIFFTTK 209

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BLAST of CsGy4G013150.1 vs. NCBI nr
Match: XP_023533000.1 (LOW QUALITY PROTEIN: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 181.8 bits (460), Expect = 2.4e-42
Identity = 115/213 (53.99%), Postives = 141/213 (66.20%), Query Frame = 0


Query: 1   MLN-SSIHYR-SNQAS-FAYQKSFIHRNVEPVLVHLHQGLKPNPSHIGFTNNLSVVKESS 60
           MLN   +HYR SNQA+ F YQK  +  NV+  +   H    P  S + FT+  +V+K S+
Sbjct: 1   MLNHPPLHYRCSNQATWFLYQKPLLPGNVDACVSLQHSLTPPAASPLRFTSP-NVIKPSA 60

Query: 61  --FAMTSINRPISIK-VVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMD 120
              ++ SI  P+  K VV T+++ I+ LFP FGF  RL  I N   S+ L LE +S +MD
Sbjct: 61  VHVSVPSIQSPVYTKAVVTTVQRPIHQLFPKFGFIGRLHDIIN-RRSRVLALELLSADMD 120

Query: 121 PTTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVI 180
           PTTGLEKG  KAY ++VKR+D+E I+YE   VIPADFG IG VLVEN H KE+FL DIVI
Sbjct: 121 PTTGLEKGRMKAYEQKVKREDDE-IIYEISFVIPADFGPIGAVLVENMHKKEVFLKDIVI 180

Query: 181 HGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           HGIPT   LHF CNSWI SK    DRRIFFTTK
Sbjct: 181 HGIPTG-PLHFSCNSWITSKAQCNDRRIFFTTK 209

[Close]

BLAST of CsGy4G013150.1 vs. TAIR10
Match: AT1G17420.1 (lipoxygenase 3)

HSP 1 Score: 75.9 bits (185), Expect = 3.3e-14
Identity = 42/105 (40.00%), Postives = 58/105 (55.24%), Query Frame = 0


Query: 106 LEFISNEMDPTTGLEKGTTKAYAERVKRKDN---EVIVYETKVVIPADFGAIGGVLVENE 165
           LE IS ++DP T L K +  A  +   +K     E + Y  +  + A FG+ G + V N+
Sbjct: 121 LELISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPGAITVMNK 180

Query: 166 HNKEMFLMDIVIHGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           H KE FL  I I G      +HFPCNSW+QS+    D+RIFFT +
Sbjct: 181 HQKEFFLESITIEGFALG-PVHFPCNSWVQSQKDHPDKRIFFTNQ 224

[Close]

BLAST of CsGy4G013150.1 vs. TAIR10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 68.9 bits (167), Expect = 4.0e-12
Identity = 38/105 (36.19%), Postives = 56/105 (53.33%), Query Frame = 0


Query: 106 LEFISNEMDPTTGLEKGTTKAYAERVKRKDN---EVIVYETKVVIPADFGAIGGVLVENE 165
           LE +S ++DP T   K +  A  +   +K N   E + Y  +  + + FG+ G + V N+
Sbjct: 127 LELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAEFTVDSAFGSPGAITVTNK 186

Query: 166 HNKEMFLMDIVIHGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           H KE FL  I I G      +HFPCNSW+QS+     +RI FT +
Sbjct: 187 HQKEFFLESITIEGFACG-PVHFPCNSWVQSQKDHPSKRILFTNQ 230

[Close]

BLAST of CsGy4G013150.1 vs. Swiss-Prot
Match: sp|O24370|LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.3e-19
Identity = 57/160 (35.62%), Postives = 88/160 (55.00%), Query Frame = 0


Query: 48  NNLSVVKESSFAMTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLE 107
           +N +    +   ++S  +   +K VVT+++ +N           LD I +    ++L L 
Sbjct: 51  HNYNGASTTKAVLSSTEKATGVKAVVTVQKQVN-----LNLSRGLDDIGDLL-GKSLLLW 110

Query: 108 FISNEMDPTTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEM 167
            ++ E+D  TG+EK   +AYA R +  D +   YE   VIP DFG +G +L+ENEH+KEM
Sbjct: 111 IVAAELDHKTGIEKPGIRAYAHRGRDVDGDT-HYEADFVIPQDFGEVGAILIENEHHKEM 170

Query: 168 FLMDIVIHGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           ++ +IVI G      +   CNSW+ SK    D+RIFFT K
Sbjct: 171 YVKNIVIDGF-VHGKVEITCNSWVHSKFDNPDKRIFFTNK 202

[Close]

BLAST of CsGy4G013150.1 vs. Swiss-Prot
Match: sp|P38419|LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 9.8e-16
Identity = 45/113 (39.82%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 102 QALGLEFISNEMDPTTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVEN 161
           ++L LE +S+E+D  TG EK T ++YA  V   D+ V+ YE    +P+ FG IG ++V N
Sbjct: 112 RSLSLELVSSELDAKTGKEKATVRSYAHNVDDDDHSVVTYEADFDVPSGFGPIGAIIVTN 171

Query: 162 EHNKEMFLMDIVI---HGIPTQYHLHFPCNSWIQSK--------DRRIFFTTK 204
           E  +EMFL DI +    G      L   CNSW+Q K         +RIFF  K
Sbjct: 172 ELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVGDEGTPSKRIFFANK 224

[Close]

BLAST of CsGy4G013150.1 vs. Swiss-Prot
Match: sp|Q84YK8|LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 50/145 (34.48%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 67  ISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGLEKGTTKA 126
           + +K V TIK  +  L         +D+I +    ++L LE +S+E++  TG +K T  +
Sbjct: 96  VKVKAVATIKVTVEGLLNSLRPSKAIDNIRD-LIGRSLFLELVSSELEAKTGKKKATVHS 155

Query: 127 YAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVI---HGIPTQYHL 186
           YA +V   D+ V+ YE    +P  FG IG V+V NE  +EMFL D+ +    G      L
Sbjct: 156 YAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVVTNELGQEMFLEDLNLTAGDGAGNSTVL 215

Query: 187 HFPCNSWIQSKD--------RRIFF 201
              CNSW+Q K         +RIFF
Sbjct: 216 PIRCNSWVQPKSSIDEGTPGKRIFF 239

[Close]

BLAST of CsGy4G013150.1 vs. Swiss-Prot
Match: sp|Q8GSM2|LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.4e-15
Identity = 54/152 (35.53%), Postives = 83/152 (54.61%), Query Frame = 0


Query: 57  SFAMTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPT 116
           S ++T+  R +++  +VT  Q    ++   G    LD I + +  + L LE +S+E+DP 
Sbjct: 60  STSVTTKERALTVTAIVT-AQVPTSVYVARG----LDDIQDLF-GKTLLLELVSSELDPK 119

Query: 117 TGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDI-VIH 176
           TG E+   K +A    ++      YE K+ +PA FG +G VLVENEH++EMF+ DI +I 
Sbjct: 120 TGRERERVKGFAHMTLKEG----TYEAKMSVPASFGPVGAVLVENEHHREMFIKDIKLIT 179

Query: 177 GIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           G      + F   SW+ SK    + R FFT K
Sbjct: 180 GGDESTAITFDVASWVHSKFDDPEPRAFFTVK 201

[Close]

BLAST of CsGy4G013150.1 vs. Swiss-Prot
Match: sp|Q9LNR3|LOX3_ARATH (Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-13
Identity = 42/105 (40.00%), Postives = 58/105 (55.24%), Query Frame = 0


Query: 106 LEFISNEMDPTTGLEKGTTKAYAERVKRKDN---EVIVYETKVVIPADFGAIGGVLVENE 165
           LE IS ++DP T L K +  A  +   +K     E + Y  +  + A FG+ G + V N+
Sbjct: 121 LELISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPGAITVMNK 180

Query: 166 HNKEMFLMDIVIHGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           H KE FL  I I G      +HFPCNSW+QS+    D+RIFFT +
Sbjct: 181 HQKEFFLESITIEGFALG-PVHFPCNSWVQSQKDHPDKRIFFTNQ 224

[Close]

BLAST of CsGy4G013150.1 vs. TrEMBL
Match: tr|A0A1S4DXM1|A0A1S4DXM1_CUCME (linoleate 13S-lipoxygenase 2-1, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC107991006 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.1e-100
Identity = 181/205 (88.29%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MLNSSIHYRSNQASFAYQKSFIHRNVEPVLVHLHQGLKPNPSHIGFTNNLSVVKESSFAM 60
           MLNSSI YRSNQASFAYQK FIHRN E + +HL QG KPN S IG TNNLSVVKESSFAM
Sbjct: 1   MLNSSILYRSNQASFAYQKPFIHRNFEVLSLHLDQGFKPNASRIGSTNNLSVVKESSFAM 60

Query: 61  TSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGLE 120
           TSINRPIS+KVVVTIK+CIN+LFPHFGFKPRLDHITNWW SQAL LEFIS EMDPTTGLE
Sbjct: 61  TSINRPISVKVVVTIKRCINELFPHFGFKPRLDHITNWWTSQALRLEFISVEMDPTTGLE 120

Query: 121 KGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPTQ 180
           KGTTKAYAE+VKR+DNEVIVYETKVV+PADFGAIGGVLVENE NKE+FLMDIVIHGIPTQ
Sbjct: 121 KGTTKAYAEKVKREDNEVIVYETKVVLPADFGAIGGVLVENERNKELFLMDIVIHGIPTQ 180

Query: 181 YHLHFPCNSWIQSKDRRIFFTTKVI 206
           YHLHFPCNSWIQSKD+RIFFTTKVI
Sbjct: 181 YHLHFPCNSWIQSKDKRIFFTTKVI 205

[Close]

BLAST of CsGy4G013150.1 vs. TrEMBL
Match: tr|A0A0A0KZ97|A0A0A0KZ97_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G288100 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 8.4e-81
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 60  MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL 119
           MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL
Sbjct: 1   MTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTGL 60

Query: 120 EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT 179
           EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT
Sbjct: 61  EKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIPT 120

Query: 180 QYHLHFPCNSWIQSKDRRIFFTTKVI 206
           QYHLHFPCNSWIQSKDRRIFFTTKVI
Sbjct: 121 QYHLHFPCNSWIQSKDRRIFFTTKVI 146

[Close]

BLAST of CsGy4G013150.1 vs. TrEMBL
Match: tr|A0A2P4JDM9|A0A2P4JDM9_QUESU (Lipoxygenase OS=Quercus suber OX=58331 GN=CFP56_59405 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 9.2e-27
Identity = 75/209 (35.89%), Postives = 113/209 (54.07%), Query Frame = 0


Query: 1   MLNSSIHY-RSNQASFAYQKSFIHRNVEPVL-VHLHQGLKPNPSHIGFTNNLSVVKESSF 60
           ML   +H   S +  F   K FIH N+   L + +H   + +   +    N + +K  + 
Sbjct: 1   MLKPQLHLSNSTKVPFLLHKPFIHGNLNASLPISMHSFCRKHKHSVRVGFNPNNIKAQAV 60

Query: 61  AMTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDPTTG 120
           A +S  + +S+K +VT+K  +     + G    LD I +    + L LE I  E+DP TG
Sbjct: 61  A-SSTEKSVSVKAIVTVKPTVGGFLTNLGIDRGLDDIQDVL-GKTLLLELIQAELDPKTG 120

Query: 121 LEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIHGIP 180
           LEK T K YA R K ++N V+ YE+   +P DFG IG V VENEH+ EM+L DI++ G  
Sbjct: 121 LEKETIKGYAHRTKGEENGVVKYESSFEVPVDFGEIGAVYVENEHHNEMYLQDIILDGF- 180

Query: 181 TQYHLHFPCNSWIQSK----DRRIFFTTK 204
           +   ++  CNSW+ SK     +R+FF+ K
Sbjct: 181 SNGPVYVTCNSWVHSKYDNPQKRVFFSNK 206

[Close]

BLAST of CsGy4G013150.1 vs. TrEMBL
Match: tr|A0A2N9GKV6|A0A2N9GKV6_FAGSY (Lipoxygenase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30969 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.7e-26
Identity = 77/212 (36.32%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 1   MLNSSIHY-RSNQASFAYQKSFIHRNVE---PVLVH-LHQGLKPNPSHIGFTNNLSVVKE 60
           ML   +H+  S Q  F   K FIH N     P+ +H      K N   + F+N+ ++   
Sbjct: 1   MLKPQLHHSNSTQIPFLLHKPFIHGNGNASFPISMHSFRTKNKQNVRVVRFSNSSNIKAV 60

Query: 61  SSFAMTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHITNWWPSQALGLEFISNEMDP 120
           +S    S  + +S+K VVT+K+ +   F + G +  LD I +    + L LE +  E+DP
Sbjct: 61  AS----STEKYVSVKAVVTVKRTVTGFFSNLGIERGLDDIKDLL-DKTLLLELVHTELDP 120

Query: 121 TTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGGVLVENEHNKEMFLMDIVIH 180
            TGLEK T K YA R   ++ +V+ YE+   +P DFG +G + VENEH+ EM+L DIV+ 
Sbjct: 121 KTGLEKETIKGYAHRTS-QEKDVVKYESSFEVPIDFGEVGAIFVENEHHTEMYLQDIVLD 180

Query: 181 GIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           G      +H  CNSW+ SK     +R+FF+ K
Sbjct: 181 GF-ANGPVHVNCNSWVHSKYDNPQKRVFFSNK 205

[Close]

BLAST of CsGy4G013150.1 vs. TrEMBL
Match: tr|A0A200Q875|A0A200Q875_9MAGN (Lipoxygenase OS=Macleaya cordata OX=56857 GN=BVC80_8919g24 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.7e-26
Identity = 71/171 (41.52%), Postives = 102/171 (59.65%), Query Frame = 0


Query: 38  KPNPSHI-GFTNNLSVVKESSFAMTSINRPISIKVVVTIKQCINDLFPHFGFKPRLDHIT 97
           KPN  H+   +NN+     +S   T+  + I +K +VT+K  +  +  + G    LD IT
Sbjct: 44  KPNGGHVKSISNNI----RASVTKTTTEKAIKVKGIVTVKMTVGGILSNLGLTRALDDIT 103

Query: 98  NWWPSQALGLEFISNEMDPTTGLEKGTTKAYAERVKRKDNEVIVYETKVVIPADFGAIGG 157
           +    +++ +E +S+E+DP TG EK T K YAE V +KD EV  YE + ++PA FG IG 
Sbjct: 104 D-LARKSMLIELVSSEVDPKTGKEKETIKDYAEWVSQKDQEV-KYECEFMVPASFGEIGA 163

Query: 158 VLVENEHNKEMFLMDIVIHGIPTQYHLHFPCNSWIQSK----DRRIFFTTK 204
           VLV+NEH+KE F  DIV +G+P       PC+SW+ SK    D+RIFF  K
Sbjct: 164 VLVQNEHHKEAFFKDIVFNGLPGG-PFTIPCDSWVHSKFDNPDKRIFFANK 207

The following BLAST results are available for this feature:

BLAST of CsGy4G013150.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_016900734.1	3.2e-100	88.29	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo]	[more]
KGN54139.1	1.3e-80	100.00	hypothetical protein Csa_4G288100 [Cucumis sativus]	[more]
XP_004142235.1	1.1e-79	100.00	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus]	[more]
XP_022958530.1	2.4e-42	53.99	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata]	[more]
XP_023533000.1	2.4e-42	53.99	LOW QUALITY PROTEIN: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbi...	[more]

BLAST of CsGy4G013150.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17420.1	3.3e-14	40.00	lipoxygenase 3	[more]
AT1G72520.1	4.0e-12	36.19	PLAT/LH2 domain-containing lipoxygenase family protein	[more]

BLAST of CsGy4G013150.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O24370\|LOX21_SOLTU	4.3e-19	35.63	Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO...	[more]
sp\|P38419\|LOXC1_ORYSJ	9.8e-16	39.82	Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX...	[more]
sp\|Q84YK8\|LOXC2_ORYSJ	2.2e-15	34.48	Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q8GSM2\|LOX23_HORVU	6.4e-15	35.53	Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1	[more]
sp\|Q9LNR3\|LOX3_ARATH	5.9e-13	40.00	Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX3 PE=2 SV=1	[more]

BLAST of CsGy4G013150.1 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4DXM1\|A0A1S4DXM1_CUCME	2.1e-100	88.29	linoleate 13S-lipoxygenase 2-1, chloroplastic-like OS=Cucumis melo OX=3656 GN=LO...	[more]
tr\|A0A0A0KZ97\|A0A0A0KZ97_CUCSA	8.4e-81	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G288100 PE=4 SV=1	[more]
tr\|A0A2P4JDM9\|A0A2P4JDM9_QUESU	9.2e-27	35.89	Lipoxygenase OS=Quercus suber OX=58331 GN=CFP56_59405 PE=3 SV=1	[more]
tr\|A0A2N9GKV6\|A0A2N9GKV6_FAGSY	2.7e-26	36.32	Lipoxygenase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30969 PE=3 SV=1	[more]
tr\|A0A200Q875\|A0A200Q875_9MAGN	2.7e-26	41.52	Lipoxygenase OS=Macleaya cordata OX=56857 GN=BVC80_8919g24 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0005515	protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR036392	PLAT/LH2_dom_sf
IPR000907	LipOase
IPR001024	PLAT/LH2_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006631	fatty acid metabolic process
biological_process	GO:0072330	monocarboxylic acid biosynthetic process
biological_process	GO:0051707	response to other organism
cellular_component	GO:0044434	chloroplast part
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function	GO:0005515	protein binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CsGy4G013150	CsGy4G013150	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CsGy4G013150.1.CDS.1	CsGy4G013150.1.CDS.1	CDS
CsGy4G013150.1.CDS.2	CsGy4G013150.1.CDS.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CsGy4G013150.1	CsGy4G013150.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001024	PLAT/LH2 domain	SMART	SM00308	LH2_4	coord: 67..202 e-value: 7.3E-13 score: 58.7
IPR001024	PLAT/LH2 domain	PFAM	PF01477	PLAT	coord: 142..200 e-value: 6.5E-6 score: 26.6
IPR001024	PLAT/LH2 domain	PROSITE	PS50095	PLAT	coord: 84..205 score: 11.714
None	No IPR available	GENE3D	G3DSA:2.60.60.20		coord: 70..205 e-value: 1.8E-23 score: 85.0
None	No IPR available	PANTHER	PTHR11771:SF65	SUBFAMILY NOT NAMED	coord: 16..203
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 16..203
IPR036392	PLAT/LH2 domain superfamily	SUPERFAMILY	SSF49723	Lipase/lipooxygenase domain (PLAT/LH2 domain)	coord: 69..203