Cp4.1LG16g08970.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG16g08970.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Heterogeneous nuclear ribonucleoprotein
Location	Cp4.1LG16 : 8316952 .. 8317683 (-)
Sequence length	732

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGCAAAGCGTCAAAGGATAGTTGATCAAGGATCTTCTTATTATGGGACCCCTCCGGGTTCTGGGTTTATGTATAATACAAGTCCCTATGCATGTGTTAGTCAGCCCCCACCATTTCCTGTTGTCAGACTACGGGGTCTTCCTTTTGATTGCATGGAAACTGATGTCGTCGAGTTCTTTCATGGTCTGGACGTAGTTGACATTCTTTTTGTCCACAAGAATGGAAAATTCACAGGGGAAGGCTTTTGTGTTTTGGGTTATCCTCTGCAGGTTGATTTTGCCCTTCAAAGGAACAGGCAAAACATGGGGCGGAGATACGTCGAGGTATTCAGGAGTAATCGACAAGAATACTACAAGGCCGTAGCAAATGAAGTTTTTGATGCATCGGGTGGGTCACCAAGAAGAACTGCACCCAGGTCAAAGTTAACCGATGAGGTGAAAGACTCCGCCGAACACACCGGTGTATTACGCTTGAGGGGTTTGCCATATTCCGCAGGCAAGGATGACATACTGGACTTCTTCAAAGATTTTATCTTATCAGAAGACTCCATTCATTTAACACTGAATTCAGAGGGGAGACCAAGTGGGGAAGCATTTGTGGAATTCTCAAATGAGCAAGACTCGAAGGCAGCCATGTCCAAGGATAGAATGACCTTAGGTAGCCGTTACATAGAGTTGTTCCCATCATCTCATGAAGAATTGGACGAAGCACTCTCTAGAGGACGGTGA

mRNA sequence

ATGGGTGCAAAGCGTCAAAGGATAGTTGATCAAGGATCTTCTTATTATGGGACCCCTCCGGGTTCTGGGTTTATGTATAATACAAGTCCCTATGCATGTGTTAGTCAGCCCCCACCATTTCCTGTTGTCAGACTACGGGGTCTTCCTTTTGATTGCATGGAAACTGATGTCGTCGAGTTCTTTCATGGTCTGGACGTAGTTGACATTCTTTTTGTCCACAAGAATGGAAAATTCACAGGGGAAGGCTTTTGTGTTTTGGGTTATCCTCTGCAGGTTGATTTTGCCCTTCAAAGGAACAGGCAAAACATGGGGCGGAGATACGTCGAGGTATTCAGGAGTAATCGACAAGAATACTACAAGGCCGTAGCAAATGAAGTTTTTGATGCATCGGGTGGGTCACCAAGAAGAACTGCACCCAGGTCAAAGTTAACCGATGAGGTGAAAGACTCCGCCGAACACACCGGTGTATTACGCTTGAGGGGTTTGCCATATTCCGCAGGCAAGGATGACATACTGGACTTCTTCAAAGATTTTATCTTATCAGAAGACTCCATTCATTTAACACTGAATTCAGAGGGGAGACCAAGTGGGGAAGCATTTGTGGAATTCTCAAATGAGCAAGACTCGAAGGCAGCCATGTCCAAGGATAGAATGACCTTAGGTAGCCGTTACATAGAGTTGTTCCCATCATCTCATGAAGAATTGGACGAAGCACTCTCTAGAGGACGGTGA

Coding sequence (CDS)

ATGGGTGCAAAGCGTCAAAGGATAGTTGATCAAGGATCTTCTTATTATGGGACCCCTCCGGGTTCTGGGTTTATGTATAATACAAGTCCCTATGCATGTGTTAGTCAGCCCCCACCATTTCCTGTTGTCAGACTACGGGGTCTTCCTTTTGATTGCATGGAAACTGATGTCGTCGAGTTCTTTCATGGTCTGGACGTAGTTGACATTCTTTTTGTCCACAAGAATGGAAAATTCACAGGGGAAGGCTTTTGTGTTTTGGGTTATCCTCTGCAGGTTGATTTTGCCCTTCAAAGGAACAGGCAAAACATGGGGCGGAGATACGTCGAGGTATTCAGGAGTAATCGACAAGAATACTACAAGGCCGTAGCAAATGAAGTTTTTGATGCATCGGGTGGGTCACCAAGAAGAACTGCACCCAGGTCAAAGTTAACCGATGAGGTGAAAGACTCCGCCGAACACACCGGTGTATTACGCTTGAGGGGTTTGCCATATTCCGCAGGCAAGGATGACATACTGGACTTCTTCAAAGATTTTATCTTATCAGAAGACTCCATTCATTTAACACTGAATTCAGAGGGGAGACCAAGTGGGGAAGCATTTGTGGAATTCTCAAATGAGCAAGACTCGAAGGCAGCCATGTCCAAGGATAGAATGACCTTAGGTAGCCGTTACATAGAGTTGTTCCCATCATCTCATGAAGAATTGGACGAAGCACTCTCTAGAGGACGGTGA

Protein sequence

MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEFFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYKAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALSRGR

Homology

[Close]

BLAST of Cp4.1LG16g08970.1 vs. Swiss-Prot
Match: RBM12_MOUSE (RNA-binding protein 12 OS=Mus musculus GN=Rbm12 PE=1 SV=3)

HSP 1 Score: 119.0 bits (297), Expect = 7.1e-26
Identity = 75/210 (35.71%), Postives = 111/210 (52.86%), Query Frame = 1


Query: 43  VRLRGLPFDCMETDVVEFFHGL--DVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNR 102
           V + G+PF  ME DV EFFHGL  D V +L  H  G+  G G      P     AL+RNR
Sbjct: 306 VSVHGMPFSAMENDVREFFHGLRVDAVHLLKDHV-GRNNGNGLVKFLSPQDTFEALKRNR 365

Query: 103 QNMGRRYVEVFRSNRQEYYKAVANEVFDASGGS------PRRTAPRSKLTDEVKDSAEHT 162
             M +RYVEV  +  +++  A  +  F  S G       P +T PRSK     K S   +
Sbjct: 366 MLMIQRYVEVSPATERQWVAAGGHITFKQSMGPSGQAHPPPQTLPRSKSPSGQKRSRSRS 425

Query: 163 G-----VLRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRPSGEAFVEFSNEQDS 222
                  + L+GLP+ A    ++DFFK   + EDSI++     G+ +GE FVEF N+ D 
Sbjct: 426 PHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKATGEGFVEFRNDADY 485

Query: 223 KAAMSKDRMTLGSRYIELFPSSHEELDEAL 240
           KAA+ + +  +G+R+I++ P + + + E +
Sbjct: 486 KAALCRHKQYMGNRFIQVHPITKKGMLEKI 514

[Close]

BLAST of Cp4.1LG16g08970.1 vs. Swiss-Prot
Match: HNRPF_MACFA (Heterogeneous nuclear ribonucleoprotein F OS=Macaca fascicularis GN=HNRNPF PE=2 SV=3)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 71/199 (35.68%), Postives = 115/199 (57.79%), Query Frame = 1


Query: 42  VVRLRGLPFDCMETDVVEFFHGLDVVD----ILFVH-KNGKFTGEGFCVLGYPLQVDFAL 101
           VV+LRGLP+ C   DV  F     + D    + F++ + G+ +GE F  LG    V  AL
Sbjct: 12  VVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMAL 71

Query: 102 QRNRQNMGRRYVEVFRSNRQEYYKAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGV 161
           +++R++MG RY+EVF+S+R E    +            + + P S       DSA + G 
Sbjct: 72  KKDRESMGHRYIEVFKSHRTEMDWVL------------KHSGPNSA------DSA-NDGF 131

Query: 162 LRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKD 221
           +RLRGLP+   K++I+ FF    +  + I L ++ EG+ +GEAFV+F++++ ++ A+ K 
Sbjct: 132 VRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKH 191

Query: 222 RMTLGSRYIELFPSSHEEL 236
           +  +G RYIE+F SS EE+
Sbjct: 192 KERIGHRYIEVFKSSQEEV 191

[Close]

BLAST of Cp4.1LG16g08970.1 vs. Swiss-Prot
Match: HNRPF_HUMAN (Heterogeneous nuclear ribonucleoprotein F OS=Homo sapiens GN=HNRNPF PE=1 SV=3)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 71/199 (35.68%), Postives = 115/199 (57.79%), Query Frame = 1


Query: 42  VVRLRGLPFDCMETDVVEFFHGLDVVD----ILFVH-KNGKFTGEGFCVLGYPLQVDFAL 101
           VV+LRGLP+ C   DV  F     + D    + F++ + G+ +GE F  LG    V  AL
Sbjct: 12  VVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMAL 71

Query: 102 QRNRQNMGRRYVEVFRSNRQEYYKAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGV 161
           +++R++MG RY+EVF+S+R E    +            + + P S       DSA + G 
Sbjct: 72  KKDRESMGHRYIEVFKSHRTEMDWVL------------KHSGPNSA------DSA-NDGF 131

Query: 162 LRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKD 221
           +RLRGLP+   K++I+ FF    +  + I L ++ EG+ +GEAFV+F++++ ++ A+ K 
Sbjct: 132 VRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKH 191

Query: 222 RMTLGSRYIELFPSSHEEL 236
           +  +G RYIE+F SS EE+
Sbjct: 192 KERIGHRYIEVFKSSQEEV 191

[Close]

BLAST of Cp4.1LG16g08970.1 vs. Swiss-Prot
Match: RBM12_PONAB (RNA-binding protein 12 OS=Pongo abelii GN=RBM12 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 77/224 (34.38%), Postives = 115/224 (51.34%), Query Frame = 1


Query: 35  SQPPPFPV------VRLRGLPFDCMETDVVEFFHGL--DVVDILFVHKNGKFTGEGFCVL 94
           S   P P+      V + G+PF  ME DV +FFHGL  D V +L  H  G+  G G    
Sbjct: 292 SSVKPLPINPDDLYVSVHGMPFSAMENDVRDFFHGLRVDAVHLLKDHV-GRNNGNGLVKF 351

Query: 95  GYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYKAVANEVFDASGGS------PRRTAPR 154
             P     AL+RNR  M +RYVEV  +  +++  A  +  F  + G       P +T PR
Sbjct: 352 LSPQDTFEALKRNRMLMIQRYVEVSPATERQWVAAGGHITFKQNMGPSGQTHPPPQTLPR 411

Query: 155 SKLTDEVKDSAEHTG-----VLRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRP 214
           SK     K S   +       + L+GLP+ A    ++DFFK   + EDSI++     G+ 
Sbjct: 412 SKSPSGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKA 471

Query: 215 SGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAL 240
           +GE FVEF NE D KAA+ + +  +G+R+I++ P + + + E +
Sbjct: 472 TGEGFVEFRNEADYKAALCRHKQYMGNRFIQVHPITKKGMLEKI 514

[Close]

BLAST of Cp4.1LG16g08970.1 vs. Swiss-Prot
Match: RBM12_MACMU (RNA-binding protein 12 OS=Macaca mulatta GN=RBM12 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 77/224 (34.38%), Postives = 115/224 (51.34%), Query Frame = 1


Query: 35  SQPPPFPV------VRLRGLPFDCMETDVVEFFHGL--DVVDILFVHKNGKFTGEGFCVL 94
           S   P P+      V + G+PF  ME DV +FFHGL  D V +L  H  G+  G G    
Sbjct: 292 SSVKPLPINPDDLYVSVHGMPFSAMENDVRDFFHGLRVDAVHLLKDHV-GRNNGNGLVKF 351

Query: 95  GYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYKAVANEVFDASGGS------PRRTAPR 154
             P     AL+RNR  M +RYVEV  +  +++  A  +  F  + G       P +T PR
Sbjct: 352 LSPQDTFEALKRNRMLMIQRYVEVSPATERQWVAAGGHITFKQNMGPSGQSHPPPQTLPR 411

Query: 155 SKLTDEVKDSAEHTG-----VLRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRP 214
           SK     K S   +       + L+GLP+ A    ++DFFK   + EDSI++     G+ 
Sbjct: 412 SKSPSGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKA 471

Query: 215 SGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAL 240
           +GE FVEF NE D KAA+ + +  +G+R+I++ P + + + E +
Sbjct: 472 TGEGFVEFRNEADYKAALCRHKQYMGNRFIQVHPITKKGMLEKI 514

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TrEMBL
Match: A0A0A0L205_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G291930 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 4.4e-131
Identity = 232/243 (95.47%), Postives = 237/243 (97.53%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEF 60
           MGAKRQRIVDQGSSYYGT PGSGFMYNTSPYA V+QPPPFPVVRLRGLPFDCMETDVVEF
Sbjct: 18  MGAKRQRIVDQGSSYYGTSPGSGFMYNTSPYAYVNQPPPFPVVRLRGLPFDCMETDVVEF 77

Query: 61  FHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYK 120
           FHGLD+VDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVE+FRSNRQEYYK
Sbjct: 78  FHGLDIVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEIFRSNRQEYYK 137

Query: 121 AVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFIL 180
           AVANEVFDA GGSPRR+APRSKL DEVKDSAEHTGVLRLRGLPYSAGKDDILDFFK F L
Sbjct: 138 AVANEVFDARGGSPRRSAPRSKLNDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKGFNL 197

Query: 181 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALS 240
           SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEA+S
Sbjct: 198 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAIS 257

Query: 241 RGR 244
           RGR
Sbjct: 258 RGR 260

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TrEMBL
Match: V4W0L4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016322mg PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 7.5e-115
Identity = 200/244 (81.97%), Postives = 220/244 (90.16%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSP-YACVSQPPPFPVVRLRGLPFDCMETDVVE 60
           MGAKRQR++DQG S+YGTPP S FMYN  P Y  VSQPPPFPVVRLRGLPFDC E DV E
Sbjct: 16  MGAKRQRLIDQGPSFYGTPPSSSFMYNPPPAYGYVSQPPPFPVVRLRGLPFDCTEVDVAE 75

Query: 61  FFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYY 120
           FFHGLD+VD+LFVHKN KFTGE FCVLGYPLQVDFALQRNRQNMGRRYVEVFRS RQEYY
Sbjct: 76  FFHGLDIVDVLFVHKNNKFTGEAFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSKRQEYY 135

Query: 121 KAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFI 180
           KA+ANEV D  GGSP R+ PR+K  DE KDSA HTG+LRLRGLP+SAGKDDI+DFFKDF+
Sbjct: 136 KAIANEVSDVRGGSPHRSIPRAKSHDEGKDSAVHTGILRLRGLPFSAGKDDIMDFFKDFV 195

Query: 181 LSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAL 240
           LSEDSIH+T+NS+GRP+GEAFVEF+N +DSKAAM+KDRMTLGSRYIELFPSSHEE+DEAL
Sbjct: 196 LSEDSIHITMNSDGRPTGEAFVEFANAEDSKAAMAKDRMTLGSRYIELFPSSHEEMDEAL 255

Query: 241 SRGR 244
           SRGR
Sbjct: 256 SRGR 259

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TrEMBL
Match: A0A067F1Q2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025001mg PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 7.5e-115
Identity = 200/244 (81.97%), Postives = 220/244 (90.16%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSP-YACVSQPPPFPVVRLRGLPFDCMETDVVE 60
           MGAKRQR++DQG S+YGTPP S FMYN  P Y  VSQPPPFPVVRLRGLPFDC E DV E
Sbjct: 16  MGAKRQRLIDQGPSFYGTPPSSSFMYNPPPAYGYVSQPPPFPVVRLRGLPFDCTEVDVAE 75

Query: 61  FFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYY 120
           FFHGLD+VD+LFVHKN KFTGE FCVLGYPLQVDFALQRNRQNMGRRYVEVFRS RQEYY
Sbjct: 76  FFHGLDIVDVLFVHKNNKFTGEAFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSKRQEYY 135

Query: 121 KAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFI 180
           KA+ANEV D  GGSP R+ PR+K  DE KDSA HTG+LRLRGLP+SAGKDDI+DFFKDF+
Sbjct: 136 KAIANEVSDVRGGSPHRSIPRAKSHDEGKDSAVHTGILRLRGLPFSAGKDDIMDFFKDFV 195

Query: 181 LSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAL 240
           LSEDSIH+T+NS+GRP+GEAFVEF+N +DSKAAM+KDRMTLGSRYIELFPSSHEE+DEAL
Sbjct: 196 LSEDSIHITMNSDGRPTGEAFVEFANAEDSKAAMAKDRMTLGSRYIELFPSSHEEMDEAL 255

Query: 241 SRGR 244
           SRGR
Sbjct: 256 SRGR 259

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TrEMBL
Match: W9RTH9_9ROSA (Heterogeneous nuclear ribonucleoprotein F OS=Morus notabilis GN=L484_015297 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 9.8e-115
Identity = 200/243 (82.30%), Postives = 219/243 (90.12%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEF 60
           MGAKRQR+V+QGSS+YGT PG  FMYN + +A V QPPPFPVV+LRGLPFDC E DV EF
Sbjct: 1   MGAKRQRLVEQGSSFYGTSPGPSFMYNPATFAYVGQPPPFPVVKLRGLPFDCAEADVAEF 60

Query: 61  FHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYK 120
           FHGLD+VD+LFVHK GKFTGE FCV GYPLQVDFALQRNRQNMGRRYVEVFRS RQEYYK
Sbjct: 61  FHGLDIVDVLFVHKYGKFTGEAFCVFGYPLQVDFALQRNRQNMGRRYVEVFRSKRQEYYK 120

Query: 121 AVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFIL 180
           A+A EVFDA GGSPRR  PRSK  DE KDSAEHTGVLRLRGLP+SAGKDDI+DFFKD +L
Sbjct: 121 AIAGEVFDARGGSPRRNIPRSKSYDEGKDSAEHTGVLRLRGLPFSAGKDDIIDFFKDIVL 180

Query: 181 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALS 240
           SEDSIH T+NSEGRP+GEAFVEF+N +DS+AAM+KDRMTLGSRYIELFPSSHEELDEAL+
Sbjct: 181 SEDSIHFTMNSEGRPTGEAFVEFANAEDSEAAMAKDRMTLGSRYIELFPSSHEELDEALA 240

Query: 241 RGR 244
           RGR
Sbjct: 241 RGR 243

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TrEMBL
Match: A0A061GSA0_THECC (RNA-binding family protein OS=Theobroma cacao GN=TCM_040707 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 4.9e-114
Identity = 197/245 (80.41%), Postives = 221/245 (90.20%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSP--YACVSQPPPFPVVRLRGLPFDCMETDVV 60
           MGAKRQRI+DQG S+YGT PGS FMYN  P  Y  VSQPPPFPVVRLRGLPFDC E DV 
Sbjct: 16  MGAKRQRIIDQGPSFYGTSPGSSFMYNAPPPPYTYVSQPPPFPVVRLRGLPFDCTEADVA 75

Query: 61  EFFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEY 120
           EFFHGLD+VD+LFVHKN KFTGE FC+LGYPLQVDFALQ+NRQNMGRRYVEVFRS RQEY
Sbjct: 76  EFFHGLDIVDVLFVHKNSKFTGEAFCILGYPLQVDFALQKNRQNMGRRYVEVFRSKRQEY 135

Query: 121 YKAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDF 180
           YKA+ANEV DA GGSPRR  PR+K +D+ KDSAEHTG+LRLRGLP+SAGKDDI++FFKDF
Sbjct: 136 YKAIANEVADARGGSPRRNVPRAKSSDDAKDSAEHTGILRLRGLPFSAGKDDIIEFFKDF 195

Query: 181 ILSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEA 240
           +LSED+IH+ +NSEGRPSGEAFVEF+N +DSKAAM+KDRMTLGSRYIELFPSS EE+D+A
Sbjct: 196 VLSEDAIHIIMNSEGRPSGEAFVEFANAEDSKAAMAKDRMTLGSRYIELFPSSPEEMDDA 255

Query: 241 LSRGR 244
           +SRGR
Sbjct: 256 ISRGR 260

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TAIR10
Match: AT3G20890.1 (AT3G20890.1 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 354.4 bits (908), Expect = 5.7e-98
Identity = 181/278 (65.11%), Postives = 206/278 (74.10%), Query Frame = 1


Query: 1   MGAKRQRIVDQG--SSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVV 60
           MG KRQR++DQG    +YG  P SGFMYN   +     PPPFP VRLRGLPFDC E DVV
Sbjct: 15  MGPKRQRMIDQGPPGPFYGPHPSSGFMYNPYGFVAPPPPPPFPAVRLRGLPFDCAELDVV 74

Query: 61  EFFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEY 120
           EFFHGLDVVD+LFVH+N K TGE FCVLGYPLQVDFALQ+NRQNMGRRYVEVFRS +QEY
Sbjct: 75  EFFHGLDVVDVLFVHRNNKVTGEAFCVLGYPLQVDFALQKNRQNMGRRYVEVFRSTKQEY 134

Query: 121 YKAVANEVFD------ASGG---------------------------SPRRTAPRSKLTD 180
           YKA+ANEV +      ASGG                           SPRR   R++ +D
Sbjct: 135 YKAIANEVAESRVHGMASGGGGGLGGGNGSGGGGGGGGGGGRISGGSSPRRHVQRARSSD 194

Query: 181 EVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFILSEDSIHLTLNSEGRPSGEAFVEFSN 240
           + K+  EHTG+LRLRGLP+SAGK+DILDFFKDF LSED +H+T+N EGRP+GEAFVEF N
Sbjct: 195 DGKEDIEHTGILRLRGLPFSAGKEDILDFFKDFELSEDFVHVTVNGEGRPTGEAFVEFRN 254

Query: 241 EQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALSRGR 244
            +DS+AAM KDR TLGSRYIELFPSS EEL+EALSRGR
Sbjct: 255 AEDSRAAMVKDRKTLGSRYIELFPSSVEELEEALSRGR 292

[Close]

BLAST of Cp4.1LG16g08970.1 vs. TAIR10
Match: AT5G66010.1 (AT5G66010.1 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 211.8 bits (538), Expect = 4.6e-55
Identity = 115/249 (46.18%), Postives = 163/249 (65.46%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYY--GTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVV 60
           +G+KRQR++ Q + Y   GT P S   +    YA       FPVVRLRGLPF+C + D+ 
Sbjct: 16  VGSKRQRMM-QSNPYLAVGTGPTS---FPPFGYA-----GGFPVVRLRGLPFNCADIDIF 75

Query: 61  EFFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEY 120
           EFF GL++VD+L V KNGKF+GE F V   P+QV+ ALQR+R NMGRRYVEVFR ++Q+Y
Sbjct: 76  EFFAGLNIVDVLLVSKNGKFSGEAFVVFAGPMQVEIALQRDRHNMGRRYVEVFRCSKQDY 135

Query: 121 YKAVANE----VFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDF 180
           Y AVA E     ++     P     R+K   E K+  E+T VL++RGLPYS  K  I++F
Sbjct: 136 YNAVAAEEGAYEYEVRASPPPTGPSRAKRFSE-KEKLEYTEVLKMRGLPYSVNKPQIIEF 195

Query: 181 FKDFILSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEE 240
           F  + + +  + +    +G+ +GEAFVEF   ++++ AM+KD+M++GSRY+ELFP++ EE
Sbjct: 196 FSGYKVIQGRVQVVCRPDGKATGEAFVEFETGEEARRAMAKDKMSIGSRYVELFPTTREE 254

Query: 241 LDEALSRGR 244
              A +R R
Sbjct: 256 ARRAEARSR 254

[Close]

BLAST of Cp4.1LG16g08970.1 vs. NCBI nr
Match: gi|449448717|ref|XP_004142112.1| (PREDICTED: heterogeneous nuclear ribonucleoprotein F [Cucumis sativus])

HSP 1 Score: 475.3 bits (1222), Expect = 6.3e-131
Identity = 232/243 (95.47%), Postives = 237/243 (97.53%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEF 60
           MGAKRQRIVDQGSSYYGT PGSGFMYNTSPYA V+QPPPFPVVRLRGLPFDCMETDVVEF
Sbjct: 18  MGAKRQRIVDQGSSYYGTSPGSGFMYNTSPYAYVNQPPPFPVVRLRGLPFDCMETDVVEF 77

Query: 61  FHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYK 120
           FHGLD+VDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVE+FRSNRQEYYK
Sbjct: 78  FHGLDIVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEIFRSNRQEYYK 137

Query: 121 AVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFIL 180
           AVANEVFDA GGSPRR+APRSKL DEVKDSAEHTGVLRLRGLPYSAGKDDILDFFK F L
Sbjct: 138 AVANEVFDARGGSPRRSAPRSKLNDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKGFNL 197

Query: 181 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALS 240
           SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEA+S
Sbjct: 198 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAIS 257

Query: 241 RGR 244
           RGR
Sbjct: 258 RGR 260

[Close]

BLAST of Cp4.1LG16g08970.1 vs. NCBI nr
Match: gi|1009118467|ref|XP_015875876.1| (PREDICTED: heterogeneous nuclear ribonucleoprotein F isoform X2 [Ziziphus jujuba])

HSP 1 Score: 421.8 bits (1083), Expect = 8.2e-115
Identity = 199/243 (81.89%), Postives = 221/243 (90.95%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEF 60
           M AKRQR+V+QGSS+YG  PGS FMYN +PYA V QPPPFPVV+LRGLPFDC ETDV EF
Sbjct: 1   MSAKRQRVVEQGSSFYGASPGSSFMYNPTPYAYVGQPPPFPVVKLRGLPFDCTETDVAEF 60

Query: 61  FHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYK 120
           FHGLD+VD+LFVHKNGKFTGE FCVLGYPLQVDFALQRNR NMGRRYVEVFRS RQEYYK
Sbjct: 61  FHGLDIVDVLFVHKNGKFTGEAFCVLGYPLQVDFALQRNRHNMGRRYVEVFRSKRQEYYK 120

Query: 121 AVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFIL 180
           A+A EV DA GGSPRR+ PR+K   E K++AEHTGVLRLRGLP+SAGKDDI++FFKDF+L
Sbjct: 121 AIAYEVSDARGGSPRRSIPRAKSYSEGKETAEHTGVLRLRGLPFSAGKDDIIEFFKDFVL 180

Query: 181 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALS 240
           SEDS+H T+NSEGRP+GEAFVEF+N +DSKAAM+KDRMTLGSRYIELFPSSHEELDEALS
Sbjct: 181 SEDSVHFTMNSEGRPTGEAFVEFANAEDSKAAMAKDRMTLGSRYIELFPSSHEELDEALS 240

Query: 241 RGR 244
           RGR
Sbjct: 241 RGR 243

[Close]

BLAST of Cp4.1LG16g08970.1 vs. NCBI nr
Match: gi|1009118465|ref|XP_015875875.1| (PREDICTED: heterogeneous nuclear ribonucleoprotein H isoform X1 [Ziziphus jujuba])

HSP 1 Score: 421.8 bits (1083), Expect = 8.2e-115
Identity = 199/243 (81.89%), Postives = 221/243 (90.95%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEF 60
           M AKRQR+V+QGSS+YG  PGS FMYN +PYA V QPPPFPVV+LRGLPFDC ETDV EF
Sbjct: 16  MSAKRQRVVEQGSSFYGASPGSSFMYNPTPYAYVGQPPPFPVVKLRGLPFDCTETDVAEF 75

Query: 61  FHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYK 120
           FHGLD+VD+LFVHKNGKFTGE FCVLGYPLQVDFALQRNR NMGRRYVEVFRS RQEYYK
Sbjct: 76  FHGLDIVDVLFVHKNGKFTGEAFCVLGYPLQVDFALQRNRHNMGRRYVEVFRSKRQEYYK 135

Query: 121 AVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFIL 180
           A+A EV DA GGSPRR+ PR+K   E K++AEHTGVLRLRGLP+SAGKDDI++FFKDF+L
Sbjct: 136 AIAYEVSDARGGSPRRSIPRAKSYSEGKETAEHTGVLRLRGLPFSAGKDDIIEFFKDFVL 195

Query: 181 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALS 240
           SEDS+H T+NSEGRP+GEAFVEF+N +DSKAAM+KDRMTLGSRYIELFPSSHEELDEALS
Sbjct: 196 SEDSVHFTMNSEGRPTGEAFVEFANAEDSKAAMAKDRMTLGSRYIELFPSSHEELDEALS 255

Query: 241 RGR 244
           RGR
Sbjct: 256 RGR 258

[Close]

BLAST of Cp4.1LG16g08970.1 vs. NCBI nr
Match: gi|567907621|ref|XP_006446124.1| (hypothetical protein CICLE_v10016322mg [Citrus clementina])

HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-114
Identity = 200/244 (81.97%), Postives = 220/244 (90.16%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSP-YACVSQPPPFPVVRLRGLPFDCMETDVVE 60
           MGAKRQR++DQG S+YGTPP S FMYN  P Y  VSQPPPFPVVRLRGLPFDC E DV E
Sbjct: 16  MGAKRQRLIDQGPSFYGTPPSSSFMYNPPPAYGYVSQPPPFPVVRLRGLPFDCTEVDVAE 75

Query: 61  FFHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYY 120
           FFHGLD+VD+LFVHKN KFTGE FCVLGYPLQVDFALQRNRQNMGRRYVEVFRS RQEYY
Sbjct: 76  FFHGLDIVDVLFVHKNNKFTGEAFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSKRQEYY 135

Query: 121 KAVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFI 180
           KA+ANEV D  GGSP R+ PR+K  DE KDSA HTG+LRLRGLP+SAGKDDI+DFFKDF+
Sbjct: 136 KAIANEVSDVRGGSPHRSIPRAKSHDEGKDSAVHTGILRLRGLPFSAGKDDIMDFFKDFV 195

Query: 181 LSEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEAL 240
           LSEDSIH+T+NS+GRP+GEAFVEF+N +DSKAAM+KDRMTLGSRYIELFPSSHEE+DEAL
Sbjct: 196 LSEDSIHITMNSDGRPTGEAFVEFANAEDSKAAMAKDRMTLGSRYIELFPSSHEEMDEAL 255

Query: 241 SRGR 244
           SRGR
Sbjct: 256 SRGR 259

[Close]

BLAST of Cp4.1LG16g08970.1 vs. NCBI nr
Match: gi|703121528|ref|XP_010102348.1| (Heterogeneous nuclear ribonucleoprotein F [Morus notabilis])

HSP 1 Score: 421.0 bits (1081), Expect = 1.4e-114
Identity = 200/243 (82.30%), Postives = 219/243 (90.12%), Query Frame = 1


Query: 1   MGAKRQRIVDQGSSYYGTPPGSGFMYNTSPYACVSQPPPFPVVRLRGLPFDCMETDVVEF 60
           MGAKRQR+V+QGSS+YGT PG  FMYN + +A V QPPPFPVV+LRGLPFDC E DV EF
Sbjct: 1   MGAKRQRLVEQGSSFYGTSPGPSFMYNPATFAYVGQPPPFPVVKLRGLPFDCAEADVAEF 60

Query: 61  FHGLDVVDILFVHKNGKFTGEGFCVLGYPLQVDFALQRNRQNMGRRYVEVFRSNRQEYYK 120
           FHGLD+VD+LFVHK GKFTGE FCV GYPLQVDFALQRNRQNMGRRYVEVFRS RQEYYK
Sbjct: 61  FHGLDIVDVLFVHKYGKFTGEAFCVFGYPLQVDFALQRNRQNMGRRYVEVFRSKRQEYYK 120

Query: 121 AVANEVFDASGGSPRRTAPRSKLTDEVKDSAEHTGVLRLRGLPYSAGKDDILDFFKDFIL 180
           A+A EVFDA GGSPRR  PRSK  DE KDSAEHTGVLRLRGLP+SAGKDDI+DFFKD +L
Sbjct: 121 AIAGEVFDARGGSPRRNIPRSKSYDEGKDSAEHTGVLRLRGLPFSAGKDDIIDFFKDIVL 180

Query: 181 SEDSIHLTLNSEGRPSGEAFVEFSNEQDSKAAMSKDRMTLGSRYIELFPSSHEELDEALS 240
           SEDSIH T+NSEGRP+GEAFVEF+N +DS+AAM+KDRMTLGSRYIELFPSSHEELDEAL+
Sbjct: 181 SEDSIHFTMNSEGRPTGEAFVEFANAEDSEAAMAKDRMTLGSRYIELFPSSHEELDEALA 240

Query: 241 RGR 244
           RGR
Sbjct: 241 RGR 243

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g08970.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RBM12_MOUSE	7.1e-26	35.71	RNA-binding protein 12 OS=Mus musculus GN=Rbm12 PE=1 SV=3	[more]
HNRPF_MACFA	1.2e-25	35.68	Heterogeneous nuclear ribonucleoprotein F OS=Macaca fascicularis GN=HNRNPF PE=2 ...	[more]
HNRPF_HUMAN	1.2e-25	35.68	Heterogeneous nuclear ribonucleoprotein F OS=Homo sapiens GN=HNRNPF PE=1 SV=3	[more]
RBM12_PONAB	2.7e-25	34.38	RNA-binding protein 12 OS=Pongo abelii GN=RBM12 PE=2 SV=1	[more]
RBM12_MACMU	2.7e-25	34.38	RNA-binding protein 12 OS=Macaca mulatta GN=RBM12 PE=2 SV=1	[more]

BLAST of Cp4.1LG16g08970.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L205_CUCSA	4.4e-131	95.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G291930 PE=4 SV=1	[more]
V4W0L4_9ROSI	7.5e-115	81.97	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10016322mg PE=4 SV=1	[more]
A0A067F1Q2_CITSI	7.5e-115	81.97	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025001mg PE=4 SV=1	[more]
W9RTH9_9ROSA	9.8e-115	82.30	Heterogeneous nuclear ribonucleoprotein F OS=Morus notabilis GN=L484_015297 PE=4...	[more]
A0A061GSA0_THECC	4.9e-114	80.41	RNA-binding family protein OS=Theobroma cacao GN=TCM_040707 PE=4 SV=1	[more]

BLAST of Cp4.1LG16g08970.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G20890.1	5.7e-98	65.11	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT5G66010.1	4.6e-55	46.18	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]

BLAST of Cp4.1LG16g08970.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449448717\|ref\|XP_004142112.1\|	6.3e-131	95.47	PREDICTED: heterogeneous nuclear ribonucleoprotein F [Cucumis sativus]	[more]
gi\|1009118467\|ref\|XP_015875876.1\|	8.2e-115	81.89	PREDICTED: heterogeneous nuclear ribonucleoprotein F isoform X2 [Ziziphus jujuba...	[more]
gi\|1009118465\|ref\|XP_015875875.1\|	8.2e-115	81.89	PREDICTED: heterogeneous nuclear ribonucleoprotein H isoform X1 [Ziziphus jujuba...	[more]
gi\|567907621\|ref\|XP_006446124.1\|	1.1e-114	81.97	hypothetical protein CICLE_v10016322mg [Citrus clementina]	[more]
gi\|703121528\|ref\|XP_010102348.1\|	1.4e-114	82.30	Heterogeneous nuclear ribonucleoprotein F [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding

Vocabulary: **INTERPRO**
Term	Definition
IPR012677	Nucleotide-bd_a/b_plait_sf
IPR000504	RRM_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0030529	intracellular ribonucleoprotein complex
cellular_component	GO:0019013	viral nucleocapsid
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG16g08970	Cp4.1LG16g08970	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG16g08970.1:cds:001	Cp4.1LG16g08970.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG16g08970.1	Cp4.1LG16g08970.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 158..225 score: 1.
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 42..111 score: 0.084coord: 156..228 score: 2.
IPR000504	RNA recognition motif domain	PROFILE	PS50102	RRM	coord: 155..243 score: 10
IPR012677	Nucleotide-binding alpha-beta plait domain	GENE3D	G3DSA:3.30.70.330		coord: 41..121 score: 9.1E-15coord: 141..243 score: 9.5
IPR012677	Nucleotide-binding alpha-beta plait domain	unknown	SSF54928	RNA-binding domain, RBD	coord: 34..120 score: 1.75E-14coord: 149..239 score: 1.75
None	No IPR available	PANTHER	PTHR13976	HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED	coord: 1..243 score: 4.1E
None	No IPR available	PANTHER	PTHR13976:SF40	RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN	coord: 1..243 score: 4.1E