Cp4.1LG10g00230.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG10g00230.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	RING finger protein
Location	Cp4.1LG10 : 3839902 .. 3840462 (+)
Sequence length	561

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCAGCGCAAGCTCCCGGCTACACTTACAGGCGCCTTCTTAAAGATCCCTTACTCCAACGTCCATTTGGACACCATGAAAGACTGGTTACCATATGCGTCGTCTCCGCCATCGCGGCTGCCTTCGTGCTCTCTTTGTTCATCTCCCTCTTCACTTGCTTTCGGCGTCAGCTGACCTCGGCGTCCTCTAAGCCACCCTCGTCGACATGTAGCAGGCAGCTCAGTAGCATGCCTGTATACATATATGGCGACTCGGCGCCTTATTGTTTTACGTCGTCGTCGTCCTTTGAGGCCAGGAACTGTGTCATATGCTTGGCGGAGTTCGAGCTTGGAGATGAGCTTCGTGTTCTGCCCAACTGCAACCATGCGTTCCATAAAGGTTGCATTGATCAGTGGCTGCCCCTGAGGTCGTTGCTTTGCCCGCTTTGCCGGGAGCTTTCGATAGATGATATTGGGAGCATGAGGAAGCCCGTTTGTACCAATCATTGGGCCCGAAACCCGGCCTTCGTATTGGCCTTCGCCCCTGCCAGTAATGCCCCATTGCCCTCCCAATTGTAA

mRNA sequence

ATGAACTCAGCGCAAGCTCCCGGCTACACTTACAGGCGCCTTCTTAAAGATCCCTTACTCCAACGTCCATTTGGACACCATGAAAGACTGGTTACCATATGCGTCGTCTCCGCCATCGCGGCTGCCTTCGTGCTCTCTTTGTTCATCTCCCTCTTCACTTGCTTTCGGCGTCAGCTGACCTCGGCGTCCTCTAAGCCACCCTCGTCGACATGTAGCAGGCAGCTCAGTAGCATGCCTGTATACATATATGGCGACTCGGCGCCTTATTGTTTTACGTCGTCGTCGTCCTTTGAGGCCAGGAACTGTGTCATATGCTTGGCGGAGTTCGAGCTTGGAGATGAGCTTCGTGTTCTGCCCAACTGCAACCATGCGTTCCATAAAGGTTGCATTGATCAGTGGCTGCCCCTGAGGTCGTTGCTTTGCCCGCTTTGCCGGGAGCTTTCGATAGATGATATTGGGAGCATGAGGAAGCCCGTTTGTACCAATCATTGGGCCCGAAACCCGGCCTTCGTATTGGCCTTCGCCCCTGCCAGTAATGCCCCATTGCCCTCCCAATTGTAA

Coding sequence (CDS)

ATGAACTCAGCGCAAGCTCCCGGCTACACTTACAGGCGCCTTCTTAAAGATCCCTTACTCCAACGTCCATTTGGACACCATGAAAGACTGGTTACCATATGCGTCGTCTCCGCCATCGCGGCTGCCTTCGTGCTCTCTTTGTTCATCTCCCTCTTCACTTGCTTTCGGCGTCAGCTGACCTCGGCGTCCTCTAAGCCACCCTCGTCGACATGTAGCAGGCAGCTCAGTAGCATGCCTGTATACATATATGGCGACTCGGCGCCTTATTGTTTTACGTCGTCGTCGTCCTTTGAGGCCAGGAACTGTGTCATATGCTTGGCGGAGTTCGAGCTTGGAGATGAGCTTCGTGTTCTGCCCAACTGCAACCATGCGTTCCATAAAGGTTGCATTGATCAGTGGCTGCCCCTGAGGTCGTTGCTTTGCCCGCTTTGCCGGGAGCTTTCGATAGATGATATTGGGAGCATGAGGAAGCCCGTTTGTACCAATCATTGGGCCCGAAACCCGGCCTTCGTATTGGCCTTCGCCCCTGCCAGTAATGCCCCATTGCCCTCCCAATTGTAA

Protein sequence

MNSAQAPGYTYRRLLKDPLLQRPFGHHERLVTICVVSAIAAAFVLSLFISLFTCFRRQLTSASSKPPSSTCSRQLSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWARNPAFVLAFAPASNAPLPSQL

Homology

[Close]

BLAST of Cp4.1LG10g00230.1 vs. Swiss-Prot
Match: ATL13_ARATH (RING-H2 finger protein ATL13 OS=Arabidopsis thaliana GN=ATL13 PE=2 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 3.4e-12
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 1


Query: 101 NCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKP 159
           +C +CL EFE  D+LR+LP C+HAFH  CID WL L    CPLCR   + D+ S + P
Sbjct: 133 DCAVCLCEFETEDKLRLLPKCSHAFHMDCIDTWL-LSHSTCPLCRSSLLSDLSSHQDP 189

[Close]

BLAST of Cp4.1LG10g00230.1 vs. Swiss-Prot
Match: ATL57_ARATH (RING-H2 finger protein ATL57 OS=Arabidopsis thaliana GN=ATL57 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-11
Identity = 34/90 (37.78%), Postives = 50/90 (55.56%), Query Frame = 1


Query: 72  SRQLSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCID 131
           S+ + S+PVY Y        T ++     +CVICL++FE G+ ++V+P+C H FH  C+D
Sbjct: 118 SQAVRSLPVYRY--------TKAAKQRNEDCVICLSDFEEGETVKVIPHCGHVFHVDCVD 177

Query: 132 QWLPLRSLLCPLCRE---LSIDDIGSMRKP 159
            WL    + CPLCR     S  D+G    P
Sbjct: 178 TWLS-SYVTCPLCRSNQLFSDKDLGMQEPP 198

[Close]

BLAST of Cp4.1LG10g00230.1 vs. Swiss-Prot
Match: ATL72_ARATH (RING-H2 finger protein ATL72 OS=Arabidopsis thaliana GN=ATL72 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.8e-11
Identity = 33/72 (45.83%), Postives = 42/72 (58.33%), Query Frame = 1


Query: 75  LSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWL 134
           L  +PV +YG             +A  C+ICL +FE G+++RVLP CNH FH  CID WL
Sbjct: 94  LKQIPVGLYGSGI-------IDMKATECLICLGDFEDGEKVRVLPKCNHGFHVRCIDTWL 153

Query: 135 PLRSLLCPLCRE 147
             RS  CP CR+
Sbjct: 154 LSRS-SCPTCRQ 157

[Close]

BLAST of Cp4.1LG10g00230.1 vs. Swiss-Prot
Match: ATL47_ARATH (RING-H2 finger protein ATL47 OS=Arabidopsis thaliana GN=ATL47 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.0e-11
Identity = 35/91 (38.46%), Postives = 50/91 (54.95%), Query Frame = 1


Query: 55  FRRQLTSASSKPPSSTCSRQLSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDE 114
           ++RQL        S      + ++PV++Y +          + E  +C +CL EF   D+
Sbjct: 104 YQRQLQQLFHLHDSGLDQALIDALPVFLYKEI-------KGTKEPFDCAVCLCEFSEDDK 163

Query: 115 LRVLPNCNHAFHKGCIDQWLPLRSLLCPLCR 146
           LR+LPNC+HAFH  CID WL L +  CPLCR
Sbjct: 164 LRLLPNCSHAFHIDCIDTWL-LSNSTCPLCR 186

[Close]

BLAST of Cp4.1LG10g00230.1 vs. Swiss-Prot
Match: ATL74_ARATH (RING-H2 finger protein ATL74 OS=Arabidopsis thaliana GN=ATL74 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.5e-11
Identity = 44/126 (34.92%), Postives = 61/126 (48.41%), Query Frame = 1


Query: 33  ICVVSAIAAAFVLSLFI-SLFTCFRRQLTSASSKPPSSTCS-------RQLSSMPVYIYG 92
           + +++A+  A +L+L + S+  C  R     SS   + T +       R+L   PV  YG
Sbjct: 35  VIILAALLCALILALGLNSILRCAMRCGFGLSSSAAAGTVADRAGLKKRELKKFPVAEYG 94

Query: 93  DSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPLC 151
                  +      A  C ICL EF  G+ +RVLP CNH+FH  CID WL   S  CP C
Sbjct: 95  -------SGEVKIAATECAICLGEFADGERVRVLPPCNHSFHMSCIDTWLVSHS-SCPNC 152

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TrEMBL
Match: A0A0A0KKP7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G505940 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.1e-72
Identity = 139/192 (72.40%), Postives = 157/192 (81.77%), Query Frame = 1


Query: 1   MNSAQAPGYTYRRLLKDPLLQRPFGHHERLVTICVVSAIAAAFVLSLFISLFTCFRRQLT 60
           MNS    GY+ RRLLKD L ++PFG+ ER+VTIC  SAIAA FV+SLFISLFTCFRRQL 
Sbjct: 24  MNSVDDLGYSNRRLLKDSLFEQPFGNRERIVTICATSAIAALFVISLFISLFTCFRRQLN 83

Query: 61  SASSKPPSSTCSRQL-SSMPVYIYGDSAP-----YCFTSSSSFEARNCVICLAEFELGDE 120
           S SSKP SSTCS QL ++MPVYIYGDS+      YCFTS   FE+R CVICLAEFE GDE
Sbjct: 84  SPSSKPLSSTCSSQLLTTMPVYIYGDSSSNMPSSYCFTS---FESRTCVICLAEFEYGDE 143

Query: 121 LRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWARNPAFVLAF 180
           LRVLPNC H FHKGCIDQWLPLRSLLCPLCRE +I +I +MR+PVC+N+W+RNPAF+LAF
Sbjct: 144 LRVLPNCKHVFHKGCIDQWLPLRSLLCPLCREHTIKEIETMRRPVCSNYWSRNPAFILAF 203

Query: 181 APASNAPLPSQL 187
              SNAPLPSQL
Sbjct: 204 GLGSNAPLPSQL 212

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TrEMBL
Match: A5B1V6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g03460 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.7e-15
Identity = 49/125 (39.20%), Postives = 68/125 (54.40%), Query Frame = 1


Query: 32  TICV-VSAIAAAFVLSLFISLFTCFRRQLTSASSKPPSSTCSRQLSS----------MPV 91
           TIC  V+    AFV   FI   T  R +     S+   ST S   SS          +PV
Sbjct: 27  TICAFVAGFGMAFVFGAFIVSCTIRRLRGYGDESQHHGSTASAASSSGQRRREHVVAIPV 86

Query: 92  YIYGDSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLL 146
           +IYG        +++S+E + C ICL E+E G+ + VLP C H FHK CI+QW+P++S+ 
Sbjct: 87  HIYGPGPSSSCAATTSYELQACAICLCEYENGERVSVLPRCKHMFHKDCIEQWVPVKSVN 146

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TrEMBL
Match: V4RVB6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027129mg PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.6e-14
Identity = 47/127 (37.01%), Postives = 74/127 (58.27%), Query Frame = 1


Query: 32  TICVVSAIAAAFVLSLFI---SLFTCFRRQLTSASSKPPSSTCSR----QLSSMPVYIYG 91
           T+ ++ AI     L  F+   +++ CF R   S   +     C R    +L+++PV+ YG
Sbjct: 18  TLSIIIAIIIVVCLICFLLIKTVYRCFNRSNQSPHGQQECQDCHRHREDKLAAIPVFAYG 77

Query: 92  DSAPYCFTSSSS-FEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPL 151
           D A    T+SS+ F  + C ICL ++   D +R+LP C H FHK CID+WL ++SL CP+
Sbjct: 78  DVATGLPTNSSTTFANKVCAICLDDYADTDVVRILPRCLHVFHKECIDKWLCVKSLQCPI 137

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TrEMBL
Match: A0A0D2SGF6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G194200 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.7e-13
Identity = 47/144 (32.64%), Postives = 75/144 (52.08%), Query Frame = 1


Query: 28  ERLVTICVVSAIAAAFVLSLFISLFTCFRRQLTSASSK---PPSSTCSRQLSSMPVYIYG 87
           +R+ +  +V+ I+   +   F  ++ C  R    +S +    P  +   +L  +PV +YG
Sbjct: 33  DRIWSTVMVAVISLGII---FFLIYKCIFRSPNHSSDENKTEPKPSVDSELDQIPVLVYG 92

Query: 88  DSA-----PYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSL 147
           +S      P C  + S  E   C ICL E+  G+ +RVLP C H FHK CI++WL + SL
Sbjct: 93  ESMMPSSEPSCSDTISELET--CAICLEEYVHGERVRVLPRCKHMFHKDCIEEWLEVPSL 152

Query: 148 LCPLCRELSIDD-IGSMRKPVCTN 163
            CP+CR+  ++  + S R   C N
Sbjct: 153 HCPICRDKVLEHCLQSARSNNCRN 171

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TrEMBL
Match: B9SLG1_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0686180 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.8e-13
Identity = 50/123 (40.65%), Postives = 64/123 (52.03%), Query Frame = 1


Query: 33  ICVVSAIAAAFVLSLFISLFTCFRRQLTSASSKPPSSTCSRQLS-----SMPVYIYGDSA 92
           ICVV  IA A    L         R+  SASS P  +  ++ L      S+P + YG +A
Sbjct: 92  ICVVGLIAVARCAWL---------RRTGSASSYPSQAAANKGLKKKILQSLPKFTYGSAA 151

Query: 93  PYCFTSSS-SFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRE 150
                + S  + +  C ICL EFE GDE+RVLP C H FH GCID WL   S  CP CR+
Sbjct: 152 ATAAAAGSCKYASTECAICLGEFEEGDEVRVLPQCGHGFHVGCIDTWLGSHS-SCPSCRQ 204

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TAIR10
Match: AT4G30400.1 (AT4G30400.1 RING/U-box superfamily protein)

HSP 1 Score: 73.2 bits (178), Expect = 1.9e-13
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 1


Query: 101 NCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKP 159
           +C +CL EFE  D+LR+LP C+HAFH  CID WL L    CPLCR   + D+ S + P
Sbjct: 133 DCAVCLCEFETEDKLRLLPKCSHAFHMDCIDTWL-LSHSTCPLCRSSLLSDLSSHQDP 189

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TAIR10
Match: AT2G27940.1 (AT2G27940.1 RING/U-box superfamily protein)

HSP 1 Score: 70.9 bits (172), Expect = 9.6e-13
Identity = 34/90 (37.78%), Postives = 50/90 (55.56%), Query Frame = 1


Query: 72  SRQLSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCID 131
           S+ + S+PVY Y        T ++     +CVICL++FE G+ ++V+P+C H FH  C+D
Sbjct: 118 SQAVRSLPVYRY--------TKAAKQRNEDCVICLSDFEEGETVKVIPHCGHVFHVDCVD 177

Query: 132 QWLPLRSLLCPLCRE---LSIDDIGSMRKP 159
            WL    + CPLCR     S  D+G    P
Sbjct: 178 TWLS-SYVTCPLCRSNQLFSDKDLGMQEPP 198

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TAIR10
Match: AT3G10910.1 (AT3G10910.1 RING/U-box superfamily protein)

HSP 1 Score: 69.7 bits (169), Expect = 2.1e-12
Identity = 33/72 (45.83%), Postives = 42/72 (58.33%), Query Frame = 1


Query: 75  LSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWL 134
           L  +PV +YG             +A  C+ICL +FE G+++RVLP CNH FH  CID WL
Sbjct: 94  LKQIPVGLYGSGI-------IDMKATECLICLGDFEDGEKVRVLPKCNHGFHVRCIDTWL 153

Query: 135 PLRSLLCPLCRE 147
             RS  CP CR+
Sbjct: 154 LSRS-SCPTCRQ 157

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TAIR10
Match: AT1G23980.1 (AT1G23980.1 RING/U-box superfamily protein)

HSP 1 Score: 69.3 bits (168), Expect = 2.8e-12
Identity = 35/91 (38.46%), Postives = 50/91 (54.95%), Query Frame = 1


Query: 55  FRRQLTSASSKPPSSTCSRQLSSMPVYIYGDSAPYCFTSSSSFEARNCVICLAEFELGDE 114
           ++RQL        S      + ++PV++Y +          + E  +C +CL EF   D+
Sbjct: 104 YQRQLQQLFHLHDSGLDQALIDALPVFLYKEI-------KGTKEPFDCAVCLCEFSEDDK 163

Query: 115 LRVLPNCNHAFHKGCIDQWLPLRSLLCPLCR 146
           LR+LPNC+HAFH  CID WL L +  CPLCR
Sbjct: 164 LRLLPNCSHAFHIDCIDTWL-LSNSTCPLCR 186

[Close]

BLAST of Cp4.1LG10g00230.1 vs. TAIR10
Match: AT1G76410.1 (AT1G76410.1 RING/U-box superfamily protein)

HSP 1 Score: 68.9 bits (167), Expect = 3.7e-12
Identity = 41/124 (33.06%), Postives = 57/124 (45.97%), Query Frame = 1


Query: 31  VTICVVSAIAAAFVLSLFISLFTCFRRQLTSASSKPPSSTCSRQLS-----SMPVYIYGD 90
           V +C ++ I     +S    L     R  +  +  PP +  ++ L      S+P   Y  
Sbjct: 34  VLLCALTCIIGLIAVSRCAWLRRIASRNRSDQTHPPPVAAANKGLKKKVLRSLPKLTYSP 93

Query: 91  SAPYCFTSSSSFEARNCVICLAEFELGDELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCR 150
            +P       + +   C ICL EF  GDELRVLP C H FH  CID WL   S  CP CR
Sbjct: 94  DSP------PAEKLVECAICLTEFAAGDELRVLPQCGHGFHVSCIDTWLGSHS-SCPSCR 150

[Close]

BLAST of Cp4.1LG10g00230.1 vs. NCBI nr
Match: gi|778722491|ref|XP_011658497.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis sativus])

HSP 1 Score: 280.0 bits (715), Expect = 3.0e-72
Identity = 139/192 (72.40%), Postives = 157/192 (81.77%), Query Frame = 1


Query: 1   MNSAQAPGYTYRRLLKDPLLQRPFGHHERLVTICVVSAIAAAFVLSLFISLFTCFRRQLT 60
           MNS    GY+ RRLLKD L ++PFG+ ER+VTIC  SAIAA FV+SLFISLFTCFRRQL 
Sbjct: 1   MNSVDDLGYSNRRLLKDSLFEQPFGNRERIVTICATSAIAALFVISLFISLFTCFRRQLN 60

Query: 61  SASSKPPSSTCSRQL-SSMPVYIYGDSAP-----YCFTSSSSFEARNCVICLAEFELGDE 120
           S SSKP SSTCS QL ++MPVYIYGDS+      YCFTS   FE+R CVICLAEFE GDE
Sbjct: 61  SPSSKPLSSTCSSQLLTTMPVYIYGDSSSNMPSSYCFTS---FESRTCVICLAEFEYGDE 120

Query: 121 LRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWARNPAFVLAF 180
           LRVLPNC H FHKGCIDQWLPLRSLLCPLCRE +I +I +MR+PVC+N+W+RNPAF+LAF
Sbjct: 121 LRVLPNCKHVFHKGCIDQWLPLRSLLCPLCREHTIKEIETMRRPVCSNYWSRNPAFILAF 180

Query: 181 APASNAPLPSQL 187
              SNAPLPSQL
Sbjct: 181 GLGSNAPLPSQL 189

[Close]

BLAST of Cp4.1LG10g00230.1 vs. NCBI nr
Match: gi|700193713|gb|KGN48917.1| (hypothetical protein Csa_6G505940 [Cucumis sativus])

HSP 1 Score: 280.0 bits (715), Expect = 3.0e-72
Identity = 139/192 (72.40%), Postives = 157/192 (81.77%), Query Frame = 1


Query: 1   MNSAQAPGYTYRRLLKDPLLQRPFGHHERLVTICVVSAIAAAFVLSLFISLFTCFRRQLT 60
           MNS    GY+ RRLLKD L ++PFG+ ER+VTIC  SAIAA FV+SLFISLFTCFRRQL 
Sbjct: 24  MNSVDDLGYSNRRLLKDSLFEQPFGNRERIVTICATSAIAALFVISLFISLFTCFRRQLN 83

Query: 61  SASSKPPSSTCSRQL-SSMPVYIYGDSAP-----YCFTSSSSFEARNCVICLAEFELGDE 120
           S SSKP SSTCS QL ++MPVYIYGDS+      YCFTS   FE+R CVICLAEFE GDE
Sbjct: 84  SPSSKPLSSTCSSQLLTTMPVYIYGDSSSNMPSSYCFTS---FESRTCVICLAEFEYGDE 143

Query: 121 LRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWARNPAFVLAF 180
           LRVLPNC H FHKGCIDQWLPLRSLLCPLCRE +I +I +MR+PVC+N+W+RNPAF+LAF
Sbjct: 144 LRVLPNCKHVFHKGCIDQWLPLRSLLCPLCREHTIKEIETMRRPVCSNYWSRNPAFILAF 203

Query: 181 APASNAPLPSQL 187
              SNAPLPSQL
Sbjct: 204 GLGSNAPLPSQL 212

[Close]

BLAST of Cp4.1LG10g00230.1 vs. NCBI nr
Match: gi|659080490|ref|XP_008440820.1| (PREDICTED: RING-H2 finger protein ATL72-like isoform X1 [Cucumis melo])

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-71
Identity = 140/193 (72.54%), Postives = 158/193 (81.87%), Query Frame = 1


Query: 1   MNSAQAPGYTYRRLLKDP-LLQRPFGHHERLVTICVVSAIAAAFVLSLFISLFTCFRRQL 60
           MNS    GY+ RRLLKD  L ++PFG+ ER+VTIC  SAIAA FV+SLFISLFTCFRRQL
Sbjct: 11  MNSVDDLGYSNRRLLKDSSLFEQPFGNRERIVTICATSAIAALFVISLFISLFTCFRRQL 70

Query: 61  TSASSKPPSSTCSRQL-SSMPVYIYGDSAP-----YCFTSSSSFEARNCVICLAEFELGD 120
            S SSKP SSTCS QL ++MPVYIYGDS+      YCFTS   FE+RNCVICLAEFE GD
Sbjct: 71  NSPSSKPLSSTCSSQLLTTMPVYIYGDSSSNMPSSYCFTS---FESRNCVICLAEFEYGD 130

Query: 121 ELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWARNPAFVLA 180
           ELRVLPNC H FHKGCIDQWLPLRSLLCPLCRE +I +I +MR+P+C+N+WARNPAF+LA
Sbjct: 131 ELRVLPNCKHVFHKGCIDQWLPLRSLLCPLCREHTIKEIETMRRPLCSNYWARNPAFILA 190

Query: 181 FAPASNAPLPSQL 187
           F   SNAPLPSQL
Sbjct: 191 FGLGSNAPLPSQL 200

[Close]

BLAST of Cp4.1LG10g00230.1 vs. NCBI nr
Match: gi|659080492|ref|XP_008440821.1| (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B isoform X2 [Cucumis melo])

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-71
Identity = 140/193 (72.54%), Postives = 158/193 (81.87%), Query Frame = 1


Query: 1   MNSAQAPGYTYRRLLKDP-LLQRPFGHHERLVTICVVSAIAAAFVLSLFISLFTCFRRQL 60
           MNS    GY+ RRLLKD  L ++PFG+ ER+VTIC  SAIAA FV+SLFISLFTCFRRQL
Sbjct: 1   MNSVDDLGYSNRRLLKDSSLFEQPFGNRERIVTICATSAIAALFVISLFISLFTCFRRQL 60

Query: 61  TSASSKPPSSTCSRQL-SSMPVYIYGDSAP-----YCFTSSSSFEARNCVICLAEFELGD 120
            S SSKP SSTCS QL ++MPVYIYGDS+      YCFTS   FE+RNCVICLAEFE GD
Sbjct: 61  NSPSSKPLSSTCSSQLLTTMPVYIYGDSSSNMPSSYCFTS---FESRNCVICLAEFEYGD 120

Query: 121 ELRVLPNCNHAFHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWARNPAFVLA 180
           ELRVLPNC H FHKGCIDQWLPLRSLLCPLCRE +I +I +MR+P+C+N+WARNPAF+LA
Sbjct: 121 ELRVLPNCKHVFHKGCIDQWLPLRSLLCPLCREHTIKEIETMRRPLCSNYWARNPAFILA 180

Query: 181 FAPASNAPLPSQL 187
           F   SNAPLPSQL
Sbjct: 181 FGLGSNAPLPSQL 190

[Close]

BLAST of Cp4.1LG10g00230.1 vs. NCBI nr
Match: gi|720049887|ref|XP_010271587.1| (PREDICTED: RING-H2 finger protein ATL74-like [Nelumbo nucifera])

HSP 1 Score: 115.9 bits (289), Expect = 7.4e-23
Identity = 75/186 (40.32%), Postives = 102/186 (54.84%), Query Frame = 1


Query: 18  PLLQRPFGHH-ERLVTICVVSAIAAAFVLSLFISLFTCFRRQLTSASSKPPSSTCS---- 77
           P L   +G + ER + +   S +    ++ LF S+F C RR   S SS P SS  S    
Sbjct: 6   PTLLPGYGSNLERTLIVLSSSFVVMMLIVFLFKSIFRCTRR--CSHSSHPQSSQSSISGT 65

Query: 78  -----RQLSSMPVYIYGDS--APYCFTSSSS-FEARNCVICLAEFELGDELRVLPNCNHA 137
                  L ++P+ IYG S  +P  F+ SS+ FE+ NC ICLAEFE G  +RVLP C H 
Sbjct: 66  IQPYSNDLDAIPISIYGASPTSPPSFSCSSTPFESENCAICLAEFEYGQTVRVLPRCKHI 125

Query: 138 FHKGCIDQWLPLRSLLCPLCRELSIDDIGSMRKPVCTNHWAR----NPAFVLAFAPASNA 187
           FHK CIDQWLP RS  CP+CR+ ++++  +     C N   R    +   VL F P+ + 
Sbjct: 126 FHKDCIDQWLPARSSFCPVCRDQAVENDVNPPTTNCRNAGGRTGNGSALLVLHFTPSVH- 185

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g00230.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ATL13_ARATH	3.4e-12	51.72	RING-H2 finger protein ATL13 OS=Arabidopsis thaliana GN=ATL13 PE=2 SV=2	[more]
ATL57_ARATH	1.7e-11	37.78	RING-H2 finger protein ATL57 OS=Arabidopsis thaliana GN=ATL57 PE=2 SV=1	[more]
ATL72_ARATH	3.8e-11	45.83	RING-H2 finger protein ATL72 OS=Arabidopsis thaliana GN=ATL72 PE=2 SV=1	[more]
ATL47_ARATH	5.0e-11	38.46	RING-H2 finger protein ATL47 OS=Arabidopsis thaliana GN=ATL47 PE=2 SV=1	[more]
ATL74_ARATH	6.5e-11	34.92	RING-H2 finger protein ATL74 OS=Arabidopsis thaliana GN=ATL74 PE=2 SV=1	[more]

BLAST of Cp4.1LG10g00230.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKP7_CUCSA	2.1e-72	72.40	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G505940 PE=4 SV=1	[more]
A5B1V6_VITVI	6.7e-15	39.20	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g03460 PE=4 SV=...	[more]
V4RVB6_9ROSI	2.6e-14	37.01	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027129mg PE=4 SV=1	[more]
A0A0D2SGF6_GOSRA	3.7e-13	32.64	Uncharacterized protein OS=Gossypium raimondii GN=B456_007G194200 PE=4 SV=1	[more]
B9SLG1_RICCO	4.8e-13	40.65	Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0686180 PE=4 SV=1	[more]

BLAST of Cp4.1LG10g00230.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G30400.1	1.9e-13	51.72	RING/U-box superfamily protein	[more]
AT2G27940.1	9.6e-13	37.78	RING/U-box superfamily protein	[more]
AT3G10910.1	2.1e-12	45.83	RING/U-box superfamily protein	[more]
AT1G23980.1	2.8e-12	38.46	RING/U-box superfamily protein	[more]
AT1G76410.1	3.7e-12	33.06	RING/U-box superfamily protein	[more]

BLAST of Cp4.1LG10g00230.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778722491\|ref\|XP_011658497.1\|	3.0e-72	72.40	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis sativus]	[more]
gi\|700193713\|gb\|KGN48917.1\|	3.0e-72	72.40	hypothetical protein Csa_6G505940 [Cucumis sativus]	[more]
gi\|659080490\|ref\|XP_008440820.1\|	1.5e-71	72.54	PREDICTED: RING-H2 finger protein ATL72-like isoform X1 [Cucumis melo]	[more]
gi\|659080492\|ref\|XP_008440821.1\|	1.5e-71	72.54	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B isoform X2 [Cucumis melo]	[more]
gi\|720049887\|ref\|XP_010271587.1\|	7.4e-23	40.32	PREDICTED: RING-H2 finger protein ATL74-like [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008270	zinc ion binding
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG10g00230	Cp4.1LG10g00230	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g00230.1:cds:001	Cp4.1LG10g00230.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG10g00230.1	Cp4.1LG10g00230.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 101..145 score: 3.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 102..144 score: 7.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 102..145 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 100..146 score: 1.5
None	No IPR available	unknown	Coil	Coil	coord: 186..186 scor
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 30..145 score: 7.2
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 95..152 score: 4.42