Cp4.1LG07g05370.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG07g05370.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Calcium-binding EF-hand family protein, putative
Location	Cp4.1LG07 : 3101766 .. 3102038 (+)
Sequence length	273

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAGTAAATATGGCAAGAACAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTTCCGTGCAGTCCGTGGCATGCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTACGTATAACATAATCTAA

mRNA sequence

ATGGCTAGTAAATATGGCAAGAACAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTTCCGTGCAGTCCGTGGCATGCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTACGTATAACATAATCTAA

Coding sequence (CDS)

ATGGCTAGTAAATATGGCAAGAACAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTTCCGTGCAGTCCGTGGCATGCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTACGTATAACATAATCTAA

Protein sequence

MASKYGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHELDKVVDYAIKCTYNII

Homology

[Close]

BLAST of Cp4.1LG07g05370.1 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.6e-25
Identity = 56/86 (65.12%), Postives = 69/86 (80.23%), Query Frame = 1


Query: 5  YGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADV 64
          Y K  S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PL+RA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCTYNII 91
          +GDG+I+E ELDKVVDYAIKC YN++
Sbjct: 66 DGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Cp4.1LG07g05370.1 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.3e-16
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1


Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEH 73
          L RE+IK IF+EHD +GDGRLS+ E+ +AFG  G+ LP ++A  GM  AD NGDG+I E 
Sbjct: 10 LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 74 ELDKVVDYAIK 85
          ELDK+VDYA K
Sbjct: 70 ELDKLVDYAQK 80

[Close]

BLAST of Cp4.1LG07g05370.1 vs. TrEMBL
Match: A0A0D2QZC2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019300 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.8e-14
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 1


Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHELDK 77
           ++KA FK  D N DGRLSI+EL +AF  LGA++P +RA RG+ +AD NGDGY+++ ELD 
Sbjct: 30  QLKAAFKSFDVNKDGRLSIEELRKAFASLGAYIPGWRARRGLSVADGNGDGYVSDDELDD 89

Query: 78  VVDYAIKCTYNI 90
           +V YA+K  Y I
Sbjct: 90  LVKYAMKQGYTI 101

[Close]

BLAST of Cp4.1LG07g05370.1 vs. TrEMBL
Match: A0A061FBR8_THECC (Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_033854 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.0e-14
Identity = 40/84 (47.62%), Postives = 58/84 (69.05%), Query Frame = 1


Query: 4  KYGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIAD 63
          K  +  +  +L   E+++IFK  D+N DGRLS QEL +AF  LG+ LP +RA RG+  AD
Sbjct: 14 KCRRKTTPMLLTGTELRSIFKSFDYNQDGRLSKQELRKAFASLGSHLPGWRAGRGLHHAD 73

Query: 64 VNGDGYITEHELDKVVDYAIKCTY 88
           N DGY+++ ELD +V+YA++C Y
Sbjct: 74 ANRDGYVSDDELDDLVEYALECGY 97

[Close]

BLAST of Cp4.1LG07g05370.1 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.0e-14
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1


Query: 16 REEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHEL 75
          REE+K IF+EHD +GDG LSI EL +A G LG  +P ++A  GM   D NGDG I+E EL
Sbjct: 13 REEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDEL 72

Query: 76 DKVVDYA 83
          DK++DYA
Sbjct: 73 DKLIDYA 79

[Close]

BLAST of Cp4.1LG07g05370.1 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 47.4 bits (111), Expect = 5.5e-06
Identity = 24/72 (33.33%), Postives = 41/72 (56.94%), Query Frame = 1


Query: 8   NNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGD 67
           N+S+      E++A+F   D N DGR+S +EL ++F  LG  L    AV  + ++D +GD
Sbjct: 33  NSSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGD 92

Query: 68  GYITEHELDKVV 80
           G +   E  +++
Sbjct: 93  GMLDFEEFSQLI 104

[Close]

BLAST of Cp4.1LG07g05370.1 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-25
Identity = 56/86 (65.12%), Postives = 69/86 (80.23%), Query Frame = 1


Query: 5  YGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADV 64
          Y K  S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PL+RA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCTYNII 91
          +GDG+I+E ELDKVVDYAIKC YN++
Sbjct: 66 DGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Cp4.1LG07g05370.1 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 4.7e-23
Identity = 54/82 (65.85%), Postives = 65/82 (79.27%), Query Frame = 1


Query: 5  YGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADV 64
          Y K  S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PL+RA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCT 87
          +GDG+I+E ELDKVVDYAIK T
Sbjct: 66 DGDGFISEQELDKVVDYAIKST 87

[Close]

BLAST of Cp4.1LG07g05370.1 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 92.8 bits (229), Expect = 3.2e-16
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1


Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEH 73
          L RE+IK IF+EHD +GDGRLS+ E+ +AFG  G+ LP ++A  GM  AD NGDG+I E 
Sbjct: 10 LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 74 ELDKVVDYAIK 85
          ELDK+VDYA K
Sbjct: 70 ELDKLVDYAQK 80

[Close]

BLAST of Cp4.1LG07g05370.1 vs. NCBI nr
Match: gi|823232722|ref|XP_012449049.1| (PREDICTED: polcalcin Che a 3-like [Gossypium raimondii])

HSP 1 Score: 85.9 bits (211), Expect = 4.0e-14
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 1


Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHELDK 77
           ++KA FK  D N DGRLSI+EL +AF  LGA++P +RA RG+ +AD NGDGY+++ ELD 
Sbjct: 30  QLKAAFKSFDVNKDGRLSIEELRKAFASLGAYIPGWRARRGLSVADGNGDGYVSDDELDD 89

Query: 78  VVDYAIKCTYNI 90
           +V YA+K  Y I
Sbjct: 90  LVKYAMKQGYTI 101

[Close]

BLAST of Cp4.1LG07g05370.1 vs. NCBI nr
Match: gi|590592372|ref|XP_007017261.1| (Calcium-binding EF-hand family protein, putative [Theobroma cacao])

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-13
Identity = 40/84 (47.62%), Postives = 58/84 (69.05%), Query Frame = 1


Query: 4  KYGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIAD 63
          K  +  +  +L   E+++IFK  D+N DGRLS QEL +AF  LG+ LP +RA RG+  AD
Sbjct: 14 KCRRKTTPMLLTGTELRSIFKSFDYNQDGRLSKQELRKAFASLGSHLPGWRAGRGLHHAD 73

Query: 64 VNGDGYITEHELDKVVDYAIKCTY 88
           N DGY+++ ELD +V+YA++C Y
Sbjct: 74 ANRDGYVSDDELDDLVEYALECGY 97

The following BLAST results are available for this feature:

BLAST of Cp4.1LG07g05370.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Cp4.1LG07g05370.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKW4_CUCSA	1.6e-25	65.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	2.3e-16	60.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0D2QZC2_GOSRA	2.8e-14	55.56	Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019300 PE=4 SV=1	[more]
A0A061FBR8_THECC	8.0e-14	47.62	Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_03385...	[more]
A0A0A0LKX1_CUCSA	8.0e-14	58.21	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]

BLAST of Cp4.1LG07g05370.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76650.1	5.5e-06	33.33	calmodulin-like 38	[more]

BLAST of Cp4.1LG07g05370.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207396\|gb\|KGN62515.1\|	2.2e-25	65.12	hypothetical protein Csa_2G358860 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	4.7e-23	65.85	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207397\|gb\|KGN62516.1\|	3.2e-16	60.56	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|823232722\|ref\|XP_012449049.1\|	4.0e-14	55.56	PREDICTED: polcalcin Che a 3-like [Gossypium raimondii]	[more]
gi\|590592372\|ref\|XP_007017261.1\|	1.2e-13	47.62	Calcium-binding EF-hand family protein, putative [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG07g05370	Cp4.1LG07g05370	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG07g05370.1:cds:001	Cp4.1LG07g05370.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG07g05370.1	Cp4.1LG07g05370.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 62..79 score: 0.
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 18..47 score: 1.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 18..46 score: 1.5E-4coord: 54..82 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 14..49 score: 14.151coord: 63..85 score: 7
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 16..78 score: 1.1
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 11..76 score: 2.57
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 27..39 score: -coord: 63..75 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 8..83 score: 9.1
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 8..83 score: 9.1