Cp4.1LG00g04370.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG00g04370.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	cinnamate-4-hydroxylase
Location	Cp4.1LG00 : 16321324 .. 16323062 (-)
Sequence length	1468

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAGCGCCAGAGCAAGAGCTTATACATCATCTGCTTCAATTTTAAAGAGGAGTTCAATACCATGGATCTTCTCCTTTTGGAGAAGGCTCTGCTGGGGCTGTTTGTGGCCATGGTTGTTGCTATTACCATATCCAAGCTCAGGGGTAAACGTTTTAAACTCCCACCAGGTCCCATCCCCATACCCATATTTGGAAATTGGCTGCAGGTGGGTGATGACTTGAACCACCGCAACCTGACGGAGATGGCCAAGAAGTTCGGTGACATACTGTTGCTCAGAATGGGACAGCGGAACCTGGTGGTTGTTTCTTCACCAGAGCTGGCTAAGGAAGTGCTGCATACCCAGGGAGTTGAATTCGGATCCAGGACTAGGAACGTGGTGTTCGATATATTCACTGACAAGGGTCAGGACATGGTGTTCACAGTTTATGGCGAGCACTGGAGGAAAATGAGACGAATCATGACTGTCCCCTTTTTCACAAACAAGGTGGTGCAGCAGAACAGGCTCGGTTGGGAGGAGGAGGCTTCCAGAGTTGTGGAGGATCTCAAGAACGATCCAAAAGCTTCCACCAGTGGGGTGGTTCTGAGAAGGCGCCTGCAGCTTCTTATGTATAACAATATGTATAGGATTATGTTCGACAGGAGATTCGACAGCATGGAGGACCCTCTCTTCAACAAACTCAAGCTTATAAACGCCGAGAGGAGTCGGTTATCTCAGAGCTTTGAATACAACTACGGCGATTTCATCCCCATACTGAGGCCTTTCTTGAGAGGGTATTTGAATGTGTGTAAGGACGTCAAGGAGAGGAGGCTCAAGCTCTTCAAGGACAATTTTGTCGATGAGAGGAGGTAATAATAAGTAACAATGTTTGCCTTTTTTGCTTAATTTGCTCCTTCTGCTACTACTAAAGCTTACAATGGCATAACATACAGGAGACTTACAAGCACGATGAGCAACAAGGAGGATAGTGTCAAATGTGCCATTGATCATATCGTGGAGGCTCAAGAGAATGGTGAAATCACCGAAGCTAATGTTCTCTTCATTGTTGAGAACATCAATGTTGCCGGTAAATATGATCTGTTCATGTCTTAGAGTGATATTCTTATGTTCTGAGATTAGCTAAATTAGCATGTTGCTGATGTTTGAACATTTAGCAATCGAAACAACATTGTGGTCGATGGAGTGGGCAATTGCTGAGCTCGTGAACCACCCCAACATCCAGAAAAAGCTGAAGGATGAGCTCGATTCGGTGCTTGGACATGGCGTTCAAATTACAGAGCCCGACATTCGCAATCTGCCTTACTTGCAGGCTGTTATCAAAGAGACCATGCGGCTAAGAATGGCCATTCCTCTCCTAGTGCCTCACATGAACCTCCTAGACGCCAAGTTGGCTGGTTATGACATCCCTGCAGAGAGCAAGATTCTTGTGAACGCATGGTGGCTGGCCAACAACCCTGACCACTGGAACAAACCCCATGAATTCCGACCCGAGAGGTTCTTAGAGGAGGAGGCCGACGTGGAGCCTAGCGGGAACGACTTCAGATACCTTCCCTTCGGCGCCGGGAGGAGGAGTTGCCCGGGGATCGTCCTTGCCTTGCCCATTGTCGCCATTACTTTGGGTCGTCTCATTCAGAACTTTGAGCTGCTGCCTCCTCCTGGACAGTCCAAGATTGATACCACTGAAAAGCCTGGACAGTTCAGCTTGCAAATATTGAACCACTCCACTATTGTGGCAAAGCCA

mRNA sequence

TGAGCGCCAGAGCAAGAGCTTATACATCATCTGCTTCAATTTTAAAGAGGAGTTCAATACCATGGATCTTCTCCTTTTGGAGAAGGCTCTGCTGGGGCTGTTTGTGGCCATGGTTGTTGCTATTACCATATCCAAGCTCAGGGGTAAACGTTTTAAACTCCCACCAGGTCCCATCCCCATACCCATATTTGGAAATTGGCTGCAGGTGGGTGATGACTTGAACCACCGCAACCTGACGGAGATGGCCAAGAAGTTCGGTGACATACTGTTGCTCAGAATGGGACAGCGGAACCTGGTGGTTGTTTCTTCACCAGAGCTGGCTAAGGAAGTGCTGCATACCCAGGGAGTTGAATTCGGATCCAGGACTAGGAACGTGGTGTTCGATATATTCACTGACAAGGGTCAGGACATGGTGTTCACAGTTTATGGCGAGCACTGGAGGAAAATGAGACGAATCATGACTGTCCCCTTTTTCACAAACAAGGTGGTGCAGCAGAACAGGCTCGGTTGGGAGGAGGAGGCTTCCAGAGTTGTGGAGGATCTCAAGAACGATCCAAAAGCTTCCACCAGTGGGGTGGTTCTGAGAAGGCGCCTGCAGCTTCTTATGTATAACAATATGTATAGGATTATGTTCGACAGGAGATTCGACAGCATGGAGGACCCTCTCTTCAACAAACTCAAGCTTATAAACGCCGAGAGGAGTCGGTTATCTCAGAGCTTTGAATACAACTACGGCGATTTCATCCCCATACTGAGGCCTTTCTTGAGAGGGTATTTGAATGTGTGTAAGGACGTCAAGGAGAGGAGGCTCAAGCTCTTCAAGGACAATTTTGTCGATGAGAGGAGGAGACTTACAAGCACGATGAGCAACAAGGAGGATACAATCGAAACAACATTGTGGTCGATGGAGTGGGCAATTGCTGAGCTCGTGAACCACCCCAACATCCAGAAAAAGCTGAAGGATGAGCTCGATTCGGTGCTTGGACATGGCGTTCAAATTACAGAGCCCGACATTCGCAATCTGCCTTACTTGCAGGCTGTTATCAAAGAGACCATGCGGCTAAGAATGGCCATTCCTCTCCTAGTGCCTCACATGAACCTCCTAGACGCCAAGTTGGCTGGTTATGACATCCCTGCAGAGAGCAAGATTCTTGTGAACGCATGGTGGCTGGCCAACAACCCTGACCACTGGAACAAACCCCATGAATTCCGACCCGAGAGGTTCTTAGAGGAGGAGGCCGACGTGGAGCCTAGCGGGAACGACTTCAGATACCTTCCCTTCGGCGCCGGGAGGAGGAGTTGCCCGGGGATCGTCCTTGCCTTGCCCATTGTCGCCATTACTTTGGGTCGTCTCATTCAGAACTTTGAGCTGCTGCCTCCTCCTGGACAGTCCAAGATTGATACCACTGAAAAGCCTGGACAGTTCAGCTTGCAAATATTGAACCACTCCACTATTGTGGCAAAGCCA

Coding sequence (CDS)

ATGGATCTTCTCCTTTTGGAGAAGGCTCTGCTGGGGCTGTTTGTGGCCATGGTTGTTGCTATTACCATATCCAAGCTCAGGGGTAAACGTTTTAAACTCCCACCAGGTCCCATCCCCATACCCATATTTGGAAATTGGCTGCAGGTGGGTGATGACTTGAACCACCGCAACCTGACGGAGATGGCCAAGAAGTTCGGTGACATACTGTTGCTCAGAATGGGACAGCGGAACCTGGTGGTTGTTTCTTCACCAGAGCTGGCTAAGGAAGTGCTGCATACCCAGGGAGTTGAATTCGGATCCAGGACTAGGAACGTGGTGTTCGATATATTCACTGACAAGGGTCAGGACATGGTGTTCACAGTTTATGGCGAGCACTGGAGGAAAATGAGACGAATCATGACTGTCCCCTTTTTCACAAACAAGGTGGTGCAGCAGAACAGGCTCGGTTGGGAGGAGGAGGCTTCCAGAGTTGTGGAGGATCTCAAGAACGATCCAAAAGCTTCCACCAGTGGGGTGGTTCTGAGAAGGCGCCTGCAGCTTCTTATGTATAACAATATGTATAGGATTATGTTCGACAGGAGATTCGACAGCATGGAGGACCCTCTCTTCAACAAACTCAAGCTTATAAACGCCGAGAGGAGTCGGTTATCTCAGAGCTTTGAATACAACTACGGCGATTTCATCCCCATACTGAGGCCTTTCTTGAGAGGGTATTTGAATGTGTGTAAGGACGTCAAGGAGAGGAGGCTCAAGCTCTTCAAGGACAATTTTGTCGATGAGAGGAGGAGACTTACAAGCACGATGAGCAACAAGGAGGATACAATCGAAACAACATTGTGGTCGATGGAGTGGGCAATTGCTGAGCTCGTGAACCACCCCAACATCCAGAAAAAGCTGAAGGATGAGCTCGATTCGGTGCTTGGACATGGCGTTCAAATTACAGAGCCCGACATTCGCAATCTGCCTTACTTGCAGGCTGTTATCAAAGAGACCATGCGGCTAAGAATGGCCATTCCTCTCCTAGTGCCTCACATGAACCTCCTAGACGCCAAGTTGGCTGGTTATGACATCCCTGCAGAGAGCAAGATTCTTGTGAACGCATGGTGGCTGGCCAACAACCCTGACCACTGGAACAAACCCCATGAATTCCGACCCGAGAGGTTCTTAGAGGAGGAGGCCGACGTGGAGCCTAGCGGGAACGACTTCAGATACCTTCCCTTCGGCGCCGGGAGGAGGAGTTGCCCGGGGATCGTCCTTGCCTTGCCCATTGTCGCCATTACTTTGGGTCGTCTCATTCAGAACTTTGAGCTGCTGCCTCCTCCTGGACAGTCCAAGATTGATACCACTGAAAAGCCTGGACAGTTCAGCTTGCAAATATTGAACCACTCCACTATTGTGGCAAAGCCA

Protein sequence

MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTEMAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQLLMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLNVCKDVKERRLKLFKDNFVDERRRLTSTMSNKEDTIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPERFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKIDTTEKPGQFSLQILNHSTIVAKP

Homology

[Close]

BLAST of Cp4.1LG00g04370.1 vs. Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 9.0e-227
Identity = 391/502 (77.89%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLG FVA++VAI +SKLRGKRFKLPPGP+P+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGSFVAILVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDILLLRMGQRNLVVVSSPEL+KEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDILLLRMGQRNLVVVSSPELSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWEEEA++VVED+K +P A+T G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQVVEDVKKNPGAATHGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLFNKLK +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFNKLKALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTST--MSNK-------------------EDT----- 300
           +C++VKERRL+LFKD FVDER++L ST  M N+                   ED      
Sbjct: 241 ICQEVKERRLQLFKDYFVDERKKLASTKNMCNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+ ELD++LG G QITEPD   LPYL AV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKL G+DIPAESKILVNAWWLANNP HW  P EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSKID
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 5.8e-226
Identity = 388/502 (77.29%), Postives = 431/502 (85.86%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLG FVA++VAI +SKLRGKRFKLPPGP+P+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGSFVAVLVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSP+L+KEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWEEEA++VVED+K +P+A+T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQVVEDVKKNPEAATNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S +DPLFNKLK +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTST--MSNK-------------------EDT----- 300
           +C++VKERRL+LFKD FVDER++L ST  MSN+                   ED      
Sbjct: 241 ICQEVKERRLQLFKDYFVDERKKLASTKNMSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+ ELD++LG G QITEPD   LPYL AV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKL G+DIPAESKILVNAWWLANNP HW  P EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEE A VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSKID
Sbjct: 421 FLEEGAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. Swiss-Prot
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.3e-225
Identity = 384/502 (76.49%), Postives = 423/502 (84.26%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF+A + AI ISKLRG+RFKLPPGPIP+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFIAAITAIAISKLRGRRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FGDI LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKRFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VV+D++ +P A+  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRRNPDAAAGGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLF KLK +N ERSRL+QSFEYNYGDFIPILRPFL+GYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKERRLKLFKD FVDER +L ST S   +                           
Sbjct: 241 ICKEVKERRLKLFKDYFVDERMKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKK++DE+D VLG G Q+TEPD++ LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDRVLGPGHQVTEPDMQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP +W +P EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKRPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. Swiss-Prot
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 4.2e-224
Identity = 385/502 (76.69%), Postives = 428/502 (85.26%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF A++VA  +SKLRGK+FKLPPGPIP+P+FGNWLQVGDDLNHRNL++
Sbjct: 1   MDLLLLEKTLLGLFAAIIVASIVSKLRGKKFKLPPGPIPVPVFGNWLQVGDDLNHRNLSD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFG+I LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGEIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWEEEA+RVVED+K +P+++T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAARVVEDVKKNPESATNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S +DPLF KLK +N ERSRL+Q FEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFVKLKALNGERSRLAQGFEYNYGDFIPILRPFLRGYLR 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTST--MSNK-------------------EDT----- 300
           +CK+VKERRL+LFKD FVDER++  ST  M N                    ED      
Sbjct: 241 ICKEVKERRLQLFKDYFVDERKKFGSTKSMDNNSLKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+DEL++VLG GVQITEPD   LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRDELETVLGPGVQITEPDTYKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPL +PHMNL DAKL GYDIPAESKILVNAW+LANNP+HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLFLPHMNLHDAKLGGYDIPAESKILVNAWFLANNPEHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ IT+GRL+QNFELLPPPG+SKID
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGKSKID 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. Swiss-Prot
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 5.5e-224
Identity = 381/503 (75.75%), Postives = 423/503 (84.10%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK L+GLF+A VVAI +S LRG++FKLPPGP+P+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLIGLFLAAVVAIAVSTLRGRKFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K +P A+ SG V+RRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWESEAAAVVEDVKKNPDAAVSGTVIRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDP+F +L+ +N ERSRL+QSFEYNYGDFIPILRPFL+GYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPIFQRLRALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKEDT-------------------------- 300
           +CK+VKE RLKLFKD FVDER++L ST S   +                           
Sbjct: 241 ICKEVKETRLKLFKDYFVDERKKLGSTKSTNNNNELKCAIDHILDAQRKGEINEDNVLYI 300

Query: 301 --------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQA 360
                   IETTLWS+EW IAELVNHP IQ+KL+DE+D VLG G Q+TEPDI+ LPYLQA
Sbjct: 301 VENINVAAIETTLWSIEWGIAELVNHPEIQQKLRDEIDRVLGAGHQVTEPDIQKLPYLQA 360

Query: 361 VIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPE 420
           V+KET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPE
Sbjct: 361 VVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPE 420

Query: 421 RFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKI 470
           RF EEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQS+I
Sbjct: 421 RFFEEESLVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQI 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TrEMBL
Match: X2D8D0_IPOPU (Cinnamate 4-monooxygenase OS=Ipomoea purpurea GN=C4H PE=2 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 1.4e-226
Identity = 391/502 (77.89%), Postives = 432/502 (86.06%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLFVA++VAI +SKLRGK++KLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFVAIIVAIVVSKLRGKKYKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFGDI +LRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGDIFMLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE E + VVED+KN+P+A+T+G VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYREGWENEIASVVEDVKNNPEAATTGTVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S +DPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMS----------------------NKEDT---- 300
           +CK+VKERRL+LFKD FVDER++L+ST S                      N+++     
Sbjct: 241 ICKEVKERRLQLFKDYFVDERKKLSSTKSMDTNSLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVN+P+IQKKL+DE+D+VLG GVQITEPD   LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNNPHIQKKLRDEIDTVLGPGVQITEPDTHKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE  VE +GNDFRYLPFGAGRRSCPGI+LALPI+ I LGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEKHVEANGNDFRYLPFGAGRRSCPGIILALPILGIVLGRLVQNFELLPPPGQSKVD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TrEMBL
Match: V9I5V9_9FABA (4-coumarate:CoA ligase OS=Caragana korshinskii GN=C4H PE=2 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.8e-226
Identity = 386/502 (76.89%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+EK LL LF+A  +AITISKLRGKRFKLPPGPIP+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLIEKTLLALFIAATIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FG+ILLLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKRFGEILLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VV+D++N+P+A+  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRNNPEAAAGGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S EDPLF KLK++N ERSRL+QSFEYNYGDFIPILRP L+GYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEEDPLFQKLKVLNGERSRLAQSFEYNYGDFIPILRPLLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VK+RRL+LFKD FVDER++L ST S   +                           
Sbjct: 241 ICKEVKDRRLQLFKDYFVDERKKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKK++DE+DSVLG G Q+TEPDI+NLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDSVLGPGHQVTEPDIQNLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ IT+GRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TrEMBL
Match: F2VR43_PRUAV (Trans-cinnamate 4-monooxygenase OS=Prunus avium GN=C4H1 PE=2 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.1e-226
Identity = 389/502 (77.49%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF+A++VAI ISKLRGKRFKLPPGPIP+PIFGNW+QVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFIAIIVAIMISKLRGKRFKLPPGPIPVPIFGNWIQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT +GQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ RLGWE EA+ VVED+K  P ++T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRLGWESEAAAVVEDVKKHPGSATNGMVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLF KL+ +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFMKLRGLNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +C++VKE+R++LFKD FVDER++L+ST +   +                           
Sbjct: 241 ICEEVKEKRIRLFKDYFVDERKKLSSTKTTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+DELDSVLG GVQITEPD + LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRDELDSVLGPGVQITEPDTQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLNDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TrEMBL
Match: W9RIS7_9ROSA (Trans-cinnamate 4-monooxygenase OS=Morus notabilis GN=L484_018794 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.1e-226
Identity = 390/502 (77.69%), Postives = 426/502 (84.86%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF A++VAI ISKLRGKRFKLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFAAVIVAIVISKLRGKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLV+VSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVIVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K +P+A+ +G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAASVVEDVKKNPEAARNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRFDS EDPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFDSEEDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKE RLKLFKD FVDER++L+S  S   +                           
Sbjct: 241 ICKEVKENRLKLFKDYFVDERKKLSSPKSTDNEGLKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQ+KL+DELD+VLG GVQITEPD+  LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRDELDTVLGRGVQITEPDMLKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP +W  P EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FL+EEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPP GQSKID
Sbjct: 421 FLQEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPAGQSKID 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TrEMBL
Match: B5KY03_9ROSA (Cinnamate-4-hydroxylase OS=Rubus coreanus PE=2 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 5.3e-226
Identity = 382/502 (76.10%), Postives = 432/502 (86.06%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+EK LLGLF A+VVAIT+SKLRGK+FKLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLMEKTLLGLFAAVVVAITVSKLRGKKFKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           MAKKFG++ +LRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  MAKKFGEVFMLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K  P+A+T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAAAVVEDVKKHPEAATNGMVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S +DPLF KLK +N ERSRL+QSFEYNYGDFIP+LRPFLRGYLN
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFVKLKGLNGERSRLAQSFEYNYGDFIPVLRPFLRGYLN 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKE+R++LFKD FVDER++L+ST +   +                           
Sbjct: 241 ICKEVKEKRIQLFKDYFVDERKKLSSTQATTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IET LWS+EW IAELVNHP IQKKL+DELD+VLGHGVQ+TEP+I+ LPYLQAV
Sbjct: 301 ENINVAAIETALWSIEWGIAELVNHPEIQKKLRDELDTVLGHGVQVTEPEIQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKL G+DIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQ+++D
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQTQLD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TAIR10
Match: AT2G30490.1 (AT2G30490.1 cinnamate-4-hydroxylase)

HSP 1 Score: 755.7 bits (1950), Expect = 1.6e-218
Identity = 369/502 (73.51%), Postives = 420/502 (83.67%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK+L+ +FVA+++A  ISKLRGK+ KLPPGPIPIPIFGNWLQVGDDLNHRNL +
Sbjct: 1   MDLLLLEKSLIAVFVAVILATVISKLRGKKLKLPPGPIPIPIFGNWLQVGDDLNHRNLVD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFGD+ LLRMGQRNLVVVSSP+L KEVL TQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGDLFLLRMGQRNLVVVSSPDLTKEVLLTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQNR GWE EA+ VVED+K +P ++T G+VLR+RLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNREGWEFEAASVVEDVKKNPDSATKGIVLRKRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNM+RIMFDRRF+S +DPLF +LK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMFRIMFDRRFESEDDPLFLRLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSN---------------------KED------ 300
           +C+DVK+RR+ LFK  FVDER+++ S+                         ED      
Sbjct: 241 ICQDVKDRRIALFKKYFVDERKQIASSKPTGSEGLKCAIDHILEAEQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQ KL++ELD+VLG GVQ+TEPD+  LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQSKLRNELDTVLGPGVQVTEPDLHKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKLAGYDIPAESKILVNAWWLANNP+ W KP EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPNSWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           F EEE+ VE +GNDFRY+PFG GRRSCPGI+LALPI+ IT+GR++QNFELLPPPGQSK+D
Sbjct: 421 FFEEESHVEANGNDFRYVPFGVGRRSCPGIILALPILGITIGRMVQNFELLPPPGQSKVD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TAIR10
Match: AT2G40890.1 (AT2G40890.1 cytochrome P450, family 98, subfamily A, polypeptide 3)

HSP 1 Score: 266.5 bits (680), Expect = 3.0e-71
Identity = 174/481 (36.17%), Postives = 251/481 (52.18%), Query Frame = 1


Query: 15  VAMVVAITISKLRGK-RFKLPPGPIPIPIFGNWLQVGDDLNHRNLTEMAKKFGDILLLRM 74
           VA + A+   KL  + R+K PPGP P PI GN   +   +  R   E A+ +G I+ + +
Sbjct: 8   VATIAAVVSYKLIQRLRYKFPPGPSPKPIVGNLYDI-KPVRFRCYYEWAQSYGPIISVWI 67

Query: 75  GQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFTVYGEHWRKMRRIM 134
           G    VVVSS ELAKEVL     +   R RN   + F+  GQD+++  YG H+ K+R++ 
Sbjct: 68  GSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVC 127

Query: 135 TVPFFTNKVVQQNRLGWEEEASRVVEDLKND---PKASTSGVVLRRRLQLLMYNNMYRIM 194
           T+  FT K ++  R   E+E + +VE +  D   P+    G+ LR+ L  + +NN+ R+ 
Sbjct: 128 TLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLA 187

Query: 195 FDRRFDSME---DPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLNVCKDVKE 254
           F +RF + E   D    + K I +   +L  S   +  + IP LR           +   
Sbjct: 188 FGKRFMNAEGVVDEQGLEFKAIVSNGLKLGASL--SIAEHIPWLRWMFPADEKAFAEHGA 247

Query: 255 RRLKL-----------------FKDNFVDERRRLTSTMSNKEDTIETT----LWSM---- 314
           RR +L                  K +FVD       T+ ++ D  E T    LW M    
Sbjct: 248 RRDRLTRAIMEEHTLARQKSSGAKQHFVD----ALLTLKDQYDLSEDTIIGLLWDMITAG 307

Query: 315 --------EWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKETMRL 374
                   EWA+AE++ +P +Q+K+++E D V+G    +TE D   LPYLQ V+KE+ RL
Sbjct: 308 MDTTAITAEWAMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRL 367

Query: 375 RMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPERFLEEEAD 434
               PL++PH +  D K+ GYDIP  S + VN W +A +P  W  P EFRPERFLEE  D
Sbjct: 368 HPPTPLMLPHRSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEE--D 427

Query: 435 VEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPG--QSKIDTTEKP 454
           V+  G+DFR LPFGAGRR CPG  L + +V   +  L+ +F   PP G    +ID +E P
Sbjct: 428 VDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENP 479

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TAIR10
Match: AT4G13310.1 (AT4G13310.1 cytochrome P450, family 71, subfamily A, polypeptide 20)

HSP 1 Score: 227.6 bits (579), Expect = 1.6e-59
Identity = 151/490 (30.82%), Postives = 253/490 (51.63%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           M+++L+   L  L   ++ +I + +   K F LPP P  +P+ GN  Q+     HR+L  
Sbjct: 1   MEMILITLCLTTLLALLLKSI-LKRTATKNFNLPPSPWRLPVIGNLHQLSLH-THRSLRS 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           ++ ++G ++LL  G+  +++VSS ++A +V+ T  +   +R +  V D     G+D+ F 
Sbjct: 61  LSLRYGPLMLLHFGRTPVLIVSSADVAHDVMKTHDLVCANRPKTKVVDKILSGGRDVAFA 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDL-KNDPKASTSGVVLRRRLQ 180
            YGE+WR+M+ I       NK+V+      EEE  R++E L K    +S S V L + L 
Sbjct: 121 PYGEYWRQMKSICIQNLLNNKMVRSYEKIREEEIKRMIEKLEKASCSSSPSPVNLSQILM 180

Query: 181 LLMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPF--LRG 240
            L  + + R+   R++   +D +     + N  R+  +   E+  G++IP L     +RG
Sbjct: 181 TLTNDIICRVALGRKYSGKKDGI----DVENIVRTFAALLGEFPVGEYIPSLSWIDRIRG 240

Query: 241 YLNVCKDVKERRLKLFKDNFVDERR------------RLTSTMSNKEDTIE--------- 300
            L+   +V ++R   F +  V E              +L +  S+K    E         
Sbjct: 241 -LDHKMEVVDKRFDEFLERVVKEHEEADKETRSDLVDKLLTIQSDKTGQFELEKSALKLI 300

Query: 301 ----------TTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQA 360
                     TTL  +EWA+ EL+ +P + KKL++E+ S     + +TE +   + YLQA
Sbjct: 301 IWDMFLAGTATTLSFLEWAMTELMRNPKVMKKLQEEIRSSSPQDLFVTEKEAEKMNYLQA 360

Query: 361 VIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHW-NKPHEFRP 420
           VIKE +RLR   PLLVP +   D KL GY+IPA ++++VNAW +  +   W     EF+P
Sbjct: 361 VIKEALRLRPPAPLLVPRVLSEDVKLKGYNIPAGTQVIVNAWAIQRDTTTWGTDAEEFKP 420

Query: 421 ERFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQ--NFELLPPPGQ 454
           ER L+   D +  G DF+++PFG+G+R CPGI     ++ +TL  +++  N+ +   P +
Sbjct: 421 ERHLDTNLDFQ--GQDFKFIPFGSGKRICPGIGFTSALIGVTLANIVKRFNWRMDVEPQR 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TAIR10
Match: AT1G74550.1 (AT1G74550.1 cytochrome P450, family 98, subfamily A, polypeptide 9)

HSP 1 Score: 226.9 bits (577), Expect = 2.6e-59
Identity = 153/482 (31.74%), Postives = 248/482 (51.45%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+          +++A  +  LR +   +PPGP    + GN  Q+   L  ++ +E
Sbjct: 1   MDLLLIS------LTTIIIAAYMQNLRRRGSNIPPGPPTRFLVGNLHQL-KPLWTQSFSE 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            ++ +G I+ + +G +  VVVSS +LAK+VL  +  +  +R R       T  G D++++
Sbjct: 61  WSQTYGPIISVWLGSQLAVVVSSSDLAKQVLRDKDYQLCNRHRTARM---TQNGSDLIWS 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSG-VVLRRRLQ 180
            YG H+ KMR++ T+  F+ K ++  R   E E S +V+ + ND  +     VVLR  L 
Sbjct: 121 DYGAHYVKMRKLCTLELFSLKSIECFRSMREMEVSSMVKSIFNDFMSDDQKPVVLRNYLD 180

Query: 181 LLMYNNMYRIMFDRRFDSMEDPLFNKL-----KLINAER--------SRLSQSFEYNYGD 240
            +  N + R++  + F+  +   F  +     +L  A +         RLS  F  +   
Sbjct: 181 SVALNIVSRLVIGKTFEPKDGREFRSIVERETRLPGATKMLDYTVWLKRLSSWFTSDKAF 240

Query: 241 FIPILRP---FLRGYLNVCKDVKERRLKLFKDNFVDERRRLTSTMSNKEDTIETTLWSM- 300
              + R    F R  ++     ++++       FV     L       E+T+   +W+M 
Sbjct: 241 MKHMARKRNWFKRAVMDEVYGGRDQKC------FVQSLLELKEKDELTEETVMGLVWNML 300

Query: 301 -----------EWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKET 360
                      EWA+AE++  P +++K++DELDSV+G G  +++ DI  LP+LQ V+KE 
Sbjct: 301 TAGADTTAITIEWAMAEMIRCPTVKEKVQDELDSVVGSGRLMSDADIPKLPFLQCVLKEA 360

Query: 361 MRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPERFLEE 420
           +RL    PL++PH      ++ GY +P  + + VN   +A +P +W+ P EFRPERFL E
Sbjct: 361 LRLHPPTPLMLPHKASESVQVGGYKVPKGATVYVNVQAIARDPANWSNPDEFRPERFLVE 420

Query: 421 EADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKIDTTEK 454
           E DV+  G DFR LPFG+GRR CP   L+L ++ + LG L+  F       +  ID TEK
Sbjct: 421 ETDVK--GQDFRVLPFGSGRRVCPAAQLSLNMMTLALGSLLHCFSWTSSTPREHIDMTEK 464

[Close]

BLAST of Cp4.1LG00g04370.1 vs. TAIR10
Match: AT4G13290.1 (AT4G13290.1 cytochrome P450, family 71, subfamily A, polypeptide 19)

HSP 1 Score: 226.9 bits (577), Expect = 2.6e-59
Identity = 142/481 (29.52%), Postives = 252/481 (52.39%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           M+++L+   L  L   +++   + +       LPP P  +P+ GN  Q+  +  HR+L  
Sbjct: 1   MEIILVTLCLTTLLALLLLKSILKRTTTNNLNLPPSPWRLPVIGNLHQLSLN-THRSLRS 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           ++ ++G ++LL  G+  +++VSS ++A ++L T  V   +R +  V D     G+D+ F 
Sbjct: 61  LSLRYGPLMLLHFGRTPVLIVSSADVAHDILKTYDVICANRPKTKVIDKILRGGRDVAFA 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
            YGE+W++M+ I      +NK+V+  +   E+E   ++E ++N    S    V   +L +
Sbjct: 121 PYGEYWKQMKSICIQNLLSNKMVRSYKKIREDEIKLMIEKVENASSCSPPSPVNLSQLFM 180

Query: 181 LMYNNMY-RIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPF--LRG 240
            + N++  R    R++ S ED +     + N  R+  +   E+  G++IP L     +RG
Sbjct: 181 TLTNDIICRAALGRKYSSKEDGI----DVENIVRAFSALVGEFPIGEYIPSLSWIDKIRG 240

Query: 241 YLNVCKDVK-------ERRLKLFKDNFVDERRRLTSTM---SNKEDTIETTLWSM----- 300
             +  ++V        ER +K  +D   D R  L  T+    + +  ++  +W M     
Sbjct: 241 QDHKMEEVDKRFDEFLERVVKEHEDANKDTRSDLVDTLLTIQSDKSALKLIIWDMFLAGT 300

Query: 301 -------EWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKETMRLR 360
                  EWA+ EL+ +P + KKL++E+ S    G+ +TE +   + YLQAVIKE +RLR
Sbjct: 301 ATSLSFLEWAMTELMRNPKVMKKLQEEIRSSSRQGLFVTEKEAEKMDYLQAVIKEALRLR 360

Query: 361 MAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWN-KPHEFRPERFLEEEAD 420
              PL+VP +   D  L GY+IPA +++++NAW +  +   W     EFRPER L+   D
Sbjct: 361 PPAPLMVPRVFSEDVTLKGYNIPAGTQVIINAWAIQRDTTTWGIDAEEFRPERHLDSILD 420

Query: 421 VEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQ--NFELLPPPGQSKIDTTEKP 454
            +  G DF+++PFG+G+R CPGI     ++ +TL  +++  N+ +   P + + D TE  
Sbjct: 421 FQ--GQDFKFIPFGSGKRICPGIGFTSALIGVTLANIVKRFNWRMDVEPQRVQHDLTEAT 474

[Close]

BLAST of Cp4.1LG00g04370.1 vs. NCBI nr
Match: gi|659114910|ref|XP_008457287.1| (PREDICTED: trans-cinnamate 4-monooxygenase-like [Cucumis melo])

HSP 1 Score: 834.7 bits (2155), Expect = 7.9e-239
Identity = 417/504 (82.74%), Postives = 445/504 (88.29%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEKAL+G+FV +VVAI+ISK++GKRF LPPGPIPIPIFGNWLQVGDDLNH NL E
Sbjct: 1   MDLLLLEKALVGMFVTIVVAISISKIKGKRFNLPPGPIPIPIFGNWLQVGDDLNHHNLRE 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT
Sbjct: 61  LAKRFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEA+ VVEDL+N+ +AST+GVVLR+RLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEAAIVVEDLRNNLEASTTGVVLRKRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYR+MFD+RFD+MEDPLFNKLKLINAERSRLSQSF+YNYGDFIPILRPFL  YLN
Sbjct: 181 LMYNNMYRMMFDKRFDNMEDPLFNKLKLINAERSRLSQSFDYNYGDFIPILRPFLTRYLN 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTS--------------------TMSNKE-------- 300
            CKDVKERRL+LFKD+FVDERRRLT                        N E        
Sbjct: 241 NCKDVKERRLRLFKDHFVDERRRLTGTMSKKKLVGGVKCAIDHIVEAQENGEITEDNVLF 300

Query: 301 -------DTIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQ 360
                    IETTLWSMEWAIAELVNHPNIQ K++DELDSVL  GVQITEPDIRNLPYLQ
Sbjct: 301 IVENINVAAIETTLWSMEWAIAELVNHPNIQSKVRDELDSVLKDGVQITEPDIRNLPYLQ 360

Query: 361 AVIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRP 420
           AVIKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNPDHWNKP EFRP
Sbjct: 361 AVIKETLRLRMAIPLLVPHMNLQDAKLGGYDIPAESKILVNAWWLANNPDHWNKPQEFRP 420

Query: 421 ERFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSK 470
           ERFLEEE +VEPSGNDFRYLPFGAGRRSCPGI+LALPI+AITLGRL+QNFEL+PPPGQSK
Sbjct: 421 ERFLEEEENVEPSGNDFRYLPFGAGRRSCPGIILALPILAITLGRLLQNFELMPPPGQSK 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. NCBI nr
Match: gi|585867810|gb|AHJ60265.1| (cinnamate 4-monooxygenase [Ipomoea purpurea])

HSP 1 Score: 793.5 bits (2048), Expect = 2.0e-226
Identity = 391/502 (77.89%), Postives = 432/502 (86.06%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLFVA++VAI +SKLRGK++KLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFVAIIVAIVVSKLRGKKYKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFGDI +LRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGDIFMLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE E + VVED+KN+P+A+T+G VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYREGWENEIASVVEDVKNNPEAATTGTVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S +DPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMS----------------------NKEDT---- 300
           +CK+VKERRL+LFKD FVDER++L+ST S                      N+++     
Sbjct: 241 ICKEVKERRLQLFKDYFVDERKKLSSTKSMDTNSLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVN+P+IQKKL+DE+D+VLG GVQITEPD   LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNNPHIQKKLRDEIDTVLGPGVQITEPDTHKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE  VE +GNDFRYLPFGAGRRSCPGI+LALPI+ I LGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEKHVEANGNDFRYLPFGAGRRSCPGIILALPILGIVLGRLVQNFELLPPPGQSKVD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. NCBI nr
Match: gi|360047692|gb|AEV93475.1| (4-coumarate:CoA ligase [Caragana korshinskii])

HSP 1 Score: 793.1 bits (2047), Expect = 2.6e-226
Identity = 386/502 (76.89%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+EK LL LF+A  +AITISKLRGKRFKLPPGPIP+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLIEKTLLALFIAATIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FG+ILLLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKRFGEILLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VV+D++N+P+A+  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRNNPEAAAGGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S EDPLF KLK++N ERSRL+QSFEYNYGDFIPILRP L+GYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEEDPLFQKLKVLNGERSRLAQSFEYNYGDFIPILRPLLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VK+RRL+LFKD FVDER++L ST S   +                           
Sbjct: 241 ICKEVKDRRLQLFKDYFVDERKKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKK++DE+DSVLG G Q+TEPDI+NLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDSVLGPGHQVTEPDIQNLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ IT+GRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. NCBI nr
Match: gi|326366169|gb|ADZ54778.1| (trans-cinnamate 4-monooxygenase [Prunus avium])

HSP 1 Score: 792.3 bits (2045), Expect = 4.5e-226
Identity = 389/502 (77.49%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF+A++VAI ISKLRGKRFKLPPGPIP+PIFGNW+QVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFIAIIVAIMISKLRGKRFKLPPGPIPVPIFGNWIQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT +GQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ RLGWE EA+ VVED+K  P ++T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRLGWESEAAAVVEDVKKHPGSATNGMVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLF KL+ +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFMKLRGLNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +C++VKE+R++LFKD FVDER++L+ST +   +                           
Sbjct: 241 ICEEVKEKRIRLFKDYFVDERKKLSSTKTTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+DELDSVLG GVQITEPD + LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRDELDSVLGPGVQITEPDTQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLNDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370.1 vs. NCBI nr
Match: gi|703091683|ref|XP_010094425.1| (Trans-cinnamate 4-monooxygenase [Morus notabilis])

HSP 1 Score: 792.0 bits (2044), Expect = 5.8e-226
Identity = 390/502 (77.69%), Postives = 426/502 (84.86%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF A++VAI ISKLRGKRFKLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFAAVIVAIVISKLRGKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLV+VSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVIVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K +P+A+ +G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAASVVEDVKKNPEAARNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRFDS EDPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFDSEEDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKE RLKLFKD FVDER++L+S  S   +                           
Sbjct: 241 ICKEVKENRLKLFKDYFVDERKKLSSPKSTDNEGLKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQ+KL+DELD+VLG GVQITEPD+  LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRDELDTVLGRGVQITEPDMLKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP +W  P EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FL+EEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPP GQSKID
Sbjct: 421 FLQEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPAGQSKID 480

The following BLAST results are available for this feature:

BLAST of Cp4.1LG00g04370.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TCMO_POPTM	9.0e-227	77.89	Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1	[more]
TCMO_POPKI	5.8e-226	77.29	Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1	[more]
TCMO_GLYEC	1.3e-225	76.49	Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1	[more]
TCMO_CATRO	4.2e-224	76.69	Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1	[more]
TCMO_SOYBN	5.5e-224	75.75	Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1	[more]

BLAST of Cp4.1LG00g04370.1 vs. TrEMBL


Match Name	E-value	Identity	Description
X2D8D0_IPOPU	1.4e-226	77.89	Cinnamate 4-monooxygenase OS=Ipomoea purpurea GN=C4H PE=2 SV=1	[more]
V9I5V9_9FABA	1.8e-226	76.89	4-coumarate:CoA ligase OS=Caragana korshinskii GN=C4H PE=2 SV=1	[more]
F2VR43_PRUAV	3.1e-226	77.49	Trans-cinnamate 4-monooxygenase OS=Prunus avium GN=C4H1 PE=2 SV=1	[more]
W9RIS7_9ROSA	4.1e-226	77.69	Trans-cinnamate 4-monooxygenase OS=Morus notabilis GN=L484_018794 PE=3 SV=1	[more]
B5KY03_9ROSA	5.3e-226	76.10	Cinnamate-4-hydroxylase OS=Rubus coreanus PE=2 SV=1	[more]

BLAST of Cp4.1LG00g04370.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G30490.1	1.6e-218	73.51	cinnamate-4-hydroxylase	[more]
AT2G40890.1	3.0e-71	36.17	cytochrome P450, family 98, subfamily A, polypeptide 3	[more]
AT4G13310.1	1.6e-59	30.82	cytochrome P450, family 71, subfamily A, polypeptide 20	[more]
AT1G74550.1	2.6e-59	31.74	cytochrome P450, family 98, subfamily A, polypeptide 9	[more]
AT4G13290.1	2.6e-59	29.52	cytochrome P450, family 71, subfamily A, polypeptide 19	[more]

BLAST of Cp4.1LG00g04370.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659114910\|ref\|XP_008457287.1\|	7.9e-239	82.74	PREDICTED: trans-cinnamate 4-monooxygenase-like [Cucumis melo]	[more]
gi\|585867810\|gb\|AHJ60265.1\|	2.0e-226	77.89	cinnamate 4-monooxygenase [Ipomoea purpurea]	[more]
gi\|360047692\|gb\|AEV93475.1\|	2.6e-226	76.89	4-coumarate:CoA ligase [Caragana korshinskii]	[more]
gi\|326366169\|gb\|ADZ54778.1\|	4.5e-226	77.49	trans-cinnamate 4-monooxygenase [Prunus avium]	[more]
gi\|703091683\|ref\|XP_010094425.1\|	5.8e-226	77.69	Trans-cinnamate 4-monooxygenase [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR017972	Cyt_P450_CS
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009805	coumarin biosynthetic process
biological_process	GO:0009809	lignin biosynthetic process
biological_process	GO:0006558	L-phenylalanine metabolic process
biological_process	GO:0009811	stilbene biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0016710	trans-cinnamate 4-monooxygenase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG00g04370	Cp4.1LG00g04370	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1_LG00_102g00080.1:five_prime_utr:001	Cp4.1_LG00_102g00080.1:five_prime_utr:001	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1_LG00_102g00080.1:cds:003	Cp4.1_LG00_102g00080.1:cds:003	CDS
Cp4.1_LG00_102g00080.1:cds:002	Cp4.1_LG00_102g00080.1:cds:002	CDS
Cp4.1_LG00_102g00080.1:cds:001	Cp4.1_LG00_102g00080.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG00g04370.1	Cp4.1LG00g04370.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 273..290 score: 3.3E-6coord: 326..337 score: 3.3E-6coord: 405..414 score: 3.
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 26..457 score: 2.4E
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 34..271 score: 1.9E-40coord: 275..462 score: 2.2
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 34..453 score: 4.45E
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 414..437 score: 4.6E-48coord: 86..107 score: 4.6E-48coord: 325..343 score: 4.6E-48coord: 366..390 score: 4.6E-48coord: 282..308 score: 4.6E-48coord: 62..81 score: 4.6E-48coord: 404..414 score: 4.6
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 407..416 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 2..469 score:
None	No IPR available	PANTHER	PTHR24298:SF56	TRANS-CINNAMATE 4-MONOOXYGENASE	coord: 2..469 score: