Cp4.1LG00g04370 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG00g04370
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	cinnamate-4-hydroxylase
Location	Cp4.1LG00 : 16321324 .. 16323062 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAGCGCCAGAGCAAGAGCTTATACATCATCTGCTTCAATTTTAAAGAGGAGTTCAATACCATGGATCTTCTCCTTTTGGAGAAGGCTCTGCTGGGGCTGTTTGTGGCCATGGTTGTTGCTATTACCATATCCAAGCTCAGGGGTAAACGTTTTAAACTCCCACCAGGTCCCATCCCCATACCCATATTTGGAAATTGGCTGCAGGTGGGTGATGACTTGAACCACCGCAACCTGACGGAGATGGCCAAGAAGTTCGGTGACATACTGTTGCTCAGAATGGGACAGCGGAACCTGGTGGTTGTTTCTTCACCAGAGCTGGCTAAGGAAGTGCTGCATACCCAGGGAGTTGAATTCGGATCCAGGACTAGGAACGTGGTGTTCGATATATTCACTGACAAGGGTCAGGACATGGTGTTCACAGTTTATGGCGAGCACTGGAGGAAAATGAGACGAATCATGACTGTCCCCTTTTTCACAAACAAGGTGGTGCAGCAGAACAGGCTCGGTTGGGAGGAGGAGGCTTCCAGAGTTGTGGAGGATCTCAAGAACGATCCAAAAGCTTCCACCAGTGGGGTGGTTCTGAGAAGGCGCCTGCAGCTTCTTATGTATAACAATATGTATAGGATTATGTTCGACAGGAGATTCGACAGCATGGAGGACCCTCTCTTCAACAAACTCAAGCTTATAAACGCCGAGAGGAGTCGGTTATCTCAGAGCTTTGAATACAACTACGGCGATTTCATCCCCATACTGAGGCCTTTCTTGAGAGGGTATTTGAATGTGTGTAAGGACGTCAAGGAGAGGAGGCTCAAGCTCTTCAAGGACAATTTTGTCGATGAGAGGAGGTAATAATAAGTAACAATGTTTGCCTTTTTTGCTTAATTTGCTCCTTCTGCTACTACTAAAGCTTACAATGGCATAACATACAGGAGACTTACAAGCACGATGAGCAACAAGGAGGATAGTGTCAAATGTGCCATTGATCATATCGTGGAGGCTCAAGAGAATGGTGAAATCACCGAAGCTAATGTTCTCTTCATTGTTGAGAACATCAATGTTGCCGGTAAATATGATCTGTTCATGTCTTAGAGTGATATTCTTATGTTCTGAGATTAGCTAAATTAGCATGTTGCTGATGTTTGAACATTTAGCAATCGAAACAACATTGTGGTCGATGGAGTGGGCAATTGCTGAGCTCGTGAACCACCCCAACATCCAGAAAAAGCTGAAGGATGAGCTCGATTCGGTGCTTGGACATGGCGTTCAAATTACAGAGCCCGACATTCGCAATCTGCCTTACTTGCAGGCTGTTATCAAAGAGACCATGCGGCTAAGAATGGCCATTCCTCTCCTAGTGCCTCACATGAACCTCCTAGACGCCAAGTTGGCTGGTTATGACATCCCTGCAGAGAGCAAGATTCTTGTGAACGCATGGTGGCTGGCCAACAACCCTGACCACTGGAACAAACCCCATGAATTCCGACCCGAGAGGTTCTTAGAGGAGGAGGCCGACGTGGAGCCTAGCGGGAACGACTTCAGATACCTTCCCTTCGGCGCCGGGAGGAGGAGTTGCCCGGGGATCGTCCTTGCCTTGCCCATTGTCGCCATTACTTTGGGTCGTCTCATTCAGAACTTTGAGCTGCTGCCTCCTCCTGGACAGTCCAAGATTGATACCACTGAAAAGCCTGGACAGTTCAGCTTGCAAATATTGAACCACTCCACTATTGTGGCAAAGCCA

mRNA sequence

TGAGCGCCAGAGCAAGAGCTTATACATCATCTGCTTCAATTTTAAAGAGGAGTTCAATACCATGGATCTTCTCCTTTTGGAGAAGGCTCTGCTGGGGCTGTTTGTGGCCATGGTTGTTGCTATTACCATATCCAAGCTCAGGGGTAAACGTTTTAAACTCCCACCAGGTCCCATCCCCATACCCATATTTGGAAATTGGCTGCAGGTGGGTGATGACTTGAACCACCGCAACCTGACGGAGATGGCCAAGAAGTTCGGTGACATACTGTTGCTCAGAATGGGACAGCGGAACCTGGTGGTTGTTTCTTCACCAGAGCTGGCTAAGGAAGTGCTGCATACCCAGGGAGTTGAATTCGGATCCAGGACTAGGAACGTGGTGTTCGATATATTCACTGACAAGGGTCAGGACATGGTGTTCACAGTTTATGGCGAGCACTGGAGGAAAATGAGACGAATCATGACTGTCCCCTTTTTCACAAACAAGGTGGTGCAGCAGAACAGGCTCGGTTGGGAGGAGGAGGCTTCCAGAGTTGTGGAGGATCTCAAGAACGATCCAAAAGCTTCCACCAGTGGGGTGGTTCTGAGAAGGCGCCTGCAGCTTCTTATGTATAACAATATGTATAGGATTATGTTCGACAGGAGATTCGACAGCATGGAGGACCCTCTCTTCAACAAACTCAAGCTTATAAACGCCGAGAGGAGTCGGTTATCTCAGAGCTTTGAATACAACTACGGCGATTTCATCCCCATACTGAGGCCTTTCTTGAGAGGGTATTTGAATGTGTGTAAGGACGTCAAGGAGAGGAGGCTCAAGCTCTTCAAGGACAATTTTGTCGATGAGAGGAGGAGACTTACAAGCACGATGAGCAACAAGGAGGATACAATCGAAACAACATTGTGGTCGATGGAGTGGGCAATTGCTGAGCTCGTGAACCACCCCAACATCCAGAAAAAGCTGAAGGATGAGCTCGATTCGGTGCTTGGACATGGCGTTCAAATTACAGAGCCCGACATTCGCAATCTGCCTTACTTGCAGGCTGTTATCAAAGAGACCATGCGGCTAAGAATGGCCATTCCTCTCCTAGTGCCTCACATGAACCTCCTAGACGCCAAGTTGGCTGGTTATGACATCCCTGCAGAGAGCAAGATTCTTGTGAACGCATGGTGGCTGGCCAACAACCCTGACCACTGGAACAAACCCCATGAATTCCGACCCGAGAGGTTCTTAGAGGAGGAGGCCGACGTGGAGCCTAGCGGGAACGACTTCAGATACCTTCCCTTCGGCGCCGGGAGGAGGAGTTGCCCGGGGATCGTCCTTGCCTTGCCCATTGTCGCCATTACTTTGGGTCGTCTCATTCAGAACTTTGAGCTGCTGCCTCCTCCTGGACAGTCCAAGATTGATACCACTGAAAAGCCTGGACAGTTCAGCTTGCAAATATTGAACCACTCCACTATTGTGGCAAAGCCA

Coding sequence (CDS)

ATGGATCTTCTCCTTTTGGAGAAGGCTCTGCTGGGGCTGTTTGTGGCCATGGTTGTTGCTATTACCATATCCAAGCTCAGGGGTAAACGTTTTAAACTCCCACCAGGTCCCATCCCCATACCCATATTTGGAAATTGGCTGCAGGTGGGTGATGACTTGAACCACCGCAACCTGACGGAGATGGCCAAGAAGTTCGGTGACATACTGTTGCTCAGAATGGGACAGCGGAACCTGGTGGTTGTTTCTTCACCAGAGCTGGCTAAGGAAGTGCTGCATACCCAGGGAGTTGAATTCGGATCCAGGACTAGGAACGTGGTGTTCGATATATTCACTGACAAGGGTCAGGACATGGTGTTCACAGTTTATGGCGAGCACTGGAGGAAAATGAGACGAATCATGACTGTCCCCTTTTTCACAAACAAGGTGGTGCAGCAGAACAGGCTCGGTTGGGAGGAGGAGGCTTCCAGAGTTGTGGAGGATCTCAAGAACGATCCAAAAGCTTCCACCAGTGGGGTGGTTCTGAGAAGGCGCCTGCAGCTTCTTATGTATAACAATATGTATAGGATTATGTTCGACAGGAGATTCGACAGCATGGAGGACCCTCTCTTCAACAAACTCAAGCTTATAAACGCCGAGAGGAGTCGGTTATCTCAGAGCTTTGAATACAACTACGGCGATTTCATCCCCATACTGAGGCCTTTCTTGAGAGGGTATTTGAATGTGTGTAAGGACGTCAAGGAGAGGAGGCTCAAGCTCTTCAAGGACAATTTTGTCGATGAGAGGAGGAGACTTACAAGCACGATGAGCAACAAGGAGGATACAATCGAAACAACATTGTGGTCGATGGAGTGGGCAATTGCTGAGCTCGTGAACCACCCCAACATCCAGAAAAAGCTGAAGGATGAGCTCGATTCGGTGCTTGGACATGGCGTTCAAATTACAGAGCCCGACATTCGCAATCTGCCTTACTTGCAGGCTGTTATCAAAGAGACCATGCGGCTAAGAATGGCCATTCCTCTCCTAGTGCCTCACATGAACCTCCTAGACGCCAAGTTGGCTGGTTATGACATCCCTGCAGAGAGCAAGATTCTTGTGAACGCATGGTGGCTGGCCAACAACCCTGACCACTGGAACAAACCCCATGAATTCCGACCCGAGAGGTTCTTAGAGGAGGAGGCCGACGTGGAGCCTAGCGGGAACGACTTCAGATACCTTCCCTTCGGCGCCGGGAGGAGGAGTTGCCCGGGGATCGTCCTTGCCTTGCCCATTGTCGCCATTACTTTGGGTCGTCTCATTCAGAACTTTGAGCTGCTGCCTCCTCCTGGACAGTCCAAGATTGATACCACTGAAAAGCCTGGACAGTTCAGCTTGCAAATATTGAACCACTCCACTATTGTGGCAAAGCCA

Protein sequence

MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTEMAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQLLMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLNVCKDVKERRLKLFKDNFVDERRRLTSTMSNKEDTIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPERFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKIDTTEKPGQFSLQILNHSTIVAKP

Homology

[Close]

BLAST of Cp4.1LG00g04370 vs. Swiss-Prot
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 9.0e-227
Identity = 391/502 (77.89%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLG FVA++VAI +SKLRGKRFKLPPGP+P+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGSFVAILVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDILLLRMGQRNLVVVSSPEL+KEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDILLLRMGQRNLVVVSSPELSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWEEEA++VVED+K +P A+T G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQVVEDVKKNPGAATHGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLFNKLK +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFNKLKALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTST--MSNK-------------------EDT----- 300
           +C++VKERRL+LFKD FVDER++L ST  M N+                   ED      
Sbjct: 241 ICQEVKERRLQLFKDYFVDERKKLASTKNMCNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+ ELD++LG G QITEPD   LPYL AV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKL G+DIPAESKILVNAWWLANNP HW  P EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSKID
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

[Close]

BLAST of Cp4.1LG00g04370 vs. Swiss-Prot
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 5.8e-226
Identity = 388/502 (77.29%), Postives = 431/502 (85.86%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLG FVA++VAI +SKLRGKRFKLPPGP+P+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGSFVAVLVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSP+L+KEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWEEEA++VVED+K +P+A+T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQVVEDVKKNPEAATNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S +DPLFNKLK +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTST--MSNK-------------------EDT----- 300
           +C++VKERRL+LFKD FVDER++L ST  MSN+                   ED      
Sbjct: 241 ICQEVKERRLQLFKDYFVDERKKLASTKNMSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+ ELD++LG G QITEPD   LPYL AV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKL G+DIPAESKILVNAWWLANNP HW  P EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEE A VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSKID
Sbjct: 421 FLEEGAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

[Close]

BLAST of Cp4.1LG00g04370 vs. Swiss-Prot
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.3e-225
Identity = 384/502 (76.49%), Postives = 423/502 (84.26%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF+A + AI ISKLRG+RFKLPPGPIP+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFIAAITAIAISKLRGRRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FGDI LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKRFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VV+D++ +P A+  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRRNPDAAAGGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLF KLK +N ERSRL+QSFEYNYGDFIPILRPFL+GYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKERRLKLFKD FVDER +L ST S   +                           
Sbjct: 241 ICKEVKERRLKLFKDYFVDERMKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKK++DE+D VLG G Q+TEPD++ LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDRVLGPGHQVTEPDMQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP +W +P EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKRPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. Swiss-Prot
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 4.2e-224
Identity = 385/502 (76.69%), Postives = 428/502 (85.26%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF A++VA  +SKLRGK+FKLPPGPIP+P+FGNWLQVGDDLNHRNL++
Sbjct: 1   MDLLLLEKTLLGLFAAIIVASIVSKLRGKKFKLPPGPIPVPVFGNWLQVGDDLNHRNLSD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFG+I LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGEIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWEEEA+RVVED+K +P+++T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAARVVEDVKKNPESATNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S +DPLF KLK +N ERSRL+Q FEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFVKLKALNGERSRLAQGFEYNYGDFIPILRPFLRGYLR 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTST--MSNK-------------------EDT----- 300
           +CK+VKERRL+LFKD FVDER++  ST  M N                    ED      
Sbjct: 241 ICKEVKERRLQLFKDYFVDERKKFGSTKSMDNNSLKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+DEL++VLG GVQITEPD   LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRDELETVLGPGVQITEPDTYKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPL +PHMNL DAKL GYDIPAESKILVNAW+LANNP+HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLFLPHMNLHDAKLGGYDIPAESKILVNAWFLANNPEHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ IT+GRL+QNFELLPPPG+SKID
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGKSKID 480

[Close]

BLAST of Cp4.1LG00g04370 vs. Swiss-Prot
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 5.5e-224
Identity = 381/503 (75.75%), Postives = 423/503 (84.10%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK L+GLF+A VVAI +S LRG++FKLPPGP+P+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLIGLFLAAVVAIAVSTLRGRKFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K +P A+ SG V+RRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWESEAAAVVEDVKKNPDAAVSGTVIRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDP+F +L+ +N ERSRL+QSFEYNYGDFIPILRPFL+GYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPIFQRLRALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKEDT-------------------------- 300
           +CK+VKE RLKLFKD FVDER++L ST S   +                           
Sbjct: 241 ICKEVKETRLKLFKDYFVDERKKLGSTKSTNNNNELKCAIDHILDAQRKGEINEDNVLYI 300

Query: 301 --------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQA 360
                   IETTLWS+EW IAELVNHP IQ+KL+DE+D VLG G Q+TEPDI+ LPYLQA
Sbjct: 301 VENINVAAIETTLWSIEWGIAELVNHPEIQQKLRDEIDRVLGAGHQVTEPDIQKLPYLQA 360

Query: 361 VIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPE 420
           V+KET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPE
Sbjct: 361 VVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPE 420

Query: 421 RFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKI 470
           RF EEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQS+I
Sbjct: 421 RFFEEESLVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQI 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TrEMBL
Match: X2D8D0_IPOPU (Cinnamate 4-monooxygenase OS=Ipomoea purpurea GN=C4H PE=2 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 1.4e-226
Identity = 391/502 (77.89%), Postives = 432/502 (86.06%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLFVA++VAI +SKLRGK++KLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFVAIIVAIVVSKLRGKKYKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFGDI +LRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGDIFMLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE E + VVED+KN+P+A+T+G VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYREGWENEIASVVEDVKNNPEAATTGTVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S +DPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMS----------------------NKEDT---- 300
           +CK+VKERRL+LFKD FVDER++L+ST S                      N+++     
Sbjct: 241 ICKEVKERRLQLFKDYFVDERKKLSSTKSMDTNSLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVN+P+IQKKL+DE+D+VLG GVQITEPD   LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNNPHIQKKLRDEIDTVLGPGVQITEPDTHKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE  VE +GNDFRYLPFGAGRRSCPGI+LALPI+ I LGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEKHVEANGNDFRYLPFGAGRRSCPGIILALPILGIVLGRLVQNFELLPPPGQSKVD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TrEMBL
Match: V9I5V9_9FABA (4-coumarate:CoA ligase OS=Caragana korshinskii GN=C4H PE=2 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.8e-226
Identity = 386/502 (76.89%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+EK LL LF+A  +AITISKLRGKRFKLPPGPIP+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLIEKTLLALFIAATIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FG+ILLLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKRFGEILLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VV+D++N+P+A+  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRNNPEAAAGGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S EDPLF KLK++N ERSRL+QSFEYNYGDFIPILRP L+GYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEEDPLFQKLKVLNGERSRLAQSFEYNYGDFIPILRPLLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VK+RRL+LFKD FVDER++L ST S   +                           
Sbjct: 241 ICKEVKDRRLQLFKDYFVDERKKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKK++DE+DSVLG G Q+TEPDI+NLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDSVLGPGHQVTEPDIQNLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ IT+GRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TrEMBL
Match: F2VR43_PRUAV (Trans-cinnamate 4-monooxygenase OS=Prunus avium GN=C4H1 PE=2 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.1e-226
Identity = 389/502 (77.49%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF+A++VAI ISKLRGKRFKLPPGPIP+PIFGNW+QVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFIAIIVAIMISKLRGKRFKLPPGPIPVPIFGNWIQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT +GQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ RLGWE EA+ VVED+K  P ++T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRLGWESEAAAVVEDVKKHPGSATNGMVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLF KL+ +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFMKLRGLNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +C++VKE+R++LFKD FVDER++L+ST +   +                           
Sbjct: 241 ICEEVKEKRIRLFKDYFVDERKKLSSTKTTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+DELDSVLG GVQITEPD + LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRDELDSVLGPGVQITEPDTQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLNDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TrEMBL
Match: W9RIS7_9ROSA (Trans-cinnamate 4-monooxygenase OS=Morus notabilis GN=L484_018794 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.1e-226
Identity = 390/502 (77.69%), Postives = 426/502 (84.86%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF A++VAI ISKLRGKRFKLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFAAVIVAIVISKLRGKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLV+VSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVIVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K +P+A+ +G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAASVVEDVKKNPEAARNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRFDS EDPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFDSEEDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKE RLKLFKD FVDER++L+S  S   +                           
Sbjct: 241 ICKEVKENRLKLFKDYFVDERKKLSSPKSTDNEGLKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQ+KL+DELD+VLG GVQITEPD+  LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRDELDTVLGRGVQITEPDMLKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP +W  P EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FL+EEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPP GQSKID
Sbjct: 421 FLQEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPAGQSKID 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TrEMBL
Match: B5KY03_9ROSA (Cinnamate-4-hydroxylase OS=Rubus coreanus PE=2 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 5.3e-226
Identity = 382/502 (76.10%), Postives = 432/502 (86.06%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+EK LLGLF A+VVAIT+SKLRGK+FKLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLMEKTLLGLFAAVVVAITVSKLRGKKFKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           MAKKFG++ +LRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  MAKKFGEVFMLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K  P+A+T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAAAVVEDVKKHPEAATNGMVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S +DPLF KLK +N ERSRL+QSFEYNYGDFIP+LRPFLRGYLN
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFVKLKGLNGERSRLAQSFEYNYGDFIPVLRPFLRGYLN 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKE+R++LFKD FVDER++L+ST +   +                           
Sbjct: 241 ICKEVKEKRIQLFKDYFVDERKKLSSTQATTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IET LWS+EW IAELVNHP IQKKL+DELD+VLGHGVQ+TEP+I+ LPYLQAV
Sbjct: 301 ENINVAAIETALWSIEWGIAELVNHPEIQKKLRDELDTVLGHGVQVTEPEIQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKL G+DIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQ+++D
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQTQLD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TAIR10
Match: AT2G30490.1 (AT2G30490.1 cinnamate-4-hydroxylase)

HSP 1 Score: 755.7 bits (1950), Expect = 1.6e-218
Identity = 369/502 (73.51%), Postives = 420/502 (83.67%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK+L+ +FVA+++A  ISKLRGK+ KLPPGPIPIPIFGNWLQVGDDLNHRNL +
Sbjct: 1   MDLLLLEKSLIAVFVAVILATVISKLRGKKLKLPPGPIPIPIFGNWLQVGDDLNHRNLVD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFGD+ LLRMGQRNLVVVSSP+L KEVL TQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGDLFLLRMGQRNLVVVSSPDLTKEVLLTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQNR GWE EA+ VVED+K +P ++T G+VLR+RLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNREGWEFEAASVVEDVKKNPDSATKGIVLRKRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNM+RIMFDRRF+S +DPLF +LK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMFRIMFDRRFESEDDPLFLRLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSN---------------------KED------ 300
           +C+DVK+RR+ LFK  FVDER+++ S+                         ED      
Sbjct: 241 ICQDVKDRRIALFKKYFVDERKQIASSKPTGSEGLKCAIDHILEAEQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQ KL++ELD+VLG GVQ+TEPD+  LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQSKLRNELDTVLGPGVQVTEPDLHKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           +KET+RLRMAIPLLVPHMNL DAKLAGYDIPAESKILVNAWWLANNP+ W KP EFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPNSWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           F EEE+ VE +GNDFRY+PFG GRRSCPGI+LALPI+ IT+GR++QNFELLPPPGQSK+D
Sbjct: 421 FFEEESHVEANGNDFRYVPFGVGRRSCPGIILALPILGITIGRMVQNFELLPPPGQSKVD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TAIR10
Match: AT2G40890.1 (AT2G40890.1 cytochrome P450, family 98, subfamily A, polypeptide 3)

HSP 1 Score: 266.5 bits (680), Expect = 3.0e-71
Identity = 174/481 (36.17%), Postives = 251/481 (52.18%), Query Frame = 1


Query: 15  VAMVVAITISKLRGK-RFKLPPGPIPIPIFGNWLQVGDDLNHRNLTEMAKKFGDILLLRM 74
           VA + A+   KL  + R+K PPGP P PI GN   +   +  R   E A+ +G I+ + +
Sbjct: 8   VATIAAVVSYKLIQRLRYKFPPGPSPKPIVGNLYDI-KPVRFRCYYEWAQSYGPIISVWI 67

Query: 75  GQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFTVYGEHWRKMRRIM 134
           G    VVVSS ELAKEVL     +   R RN   + F+  GQD+++  YG H+ K+R++ 
Sbjct: 68  GSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVC 127

Query: 135 TVPFFTNKVVQQNRLGWEEEASRVVEDLKND---PKASTSGVVLRRRLQLLMYNNMYRIM 194
           T+  FT K ++  R   E+E + +VE +  D   P+    G+ LR+ L  + +NN+ R+ 
Sbjct: 128 TLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLA 187

Query: 195 FDRRFDSME---DPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLNVCKDVKE 254
           F +RF + E   D    + K I +   +L  S   +  + IP LR           +   
Sbjct: 188 FGKRFMNAEGVVDEQGLEFKAIVSNGLKLGASL--SIAEHIPWLRWMFPADEKAFAEHGA 247

Query: 255 RRLKL-----------------FKDNFVDERRRLTSTMSNKEDTIETT----LWSM---- 314
           RR +L                  K +FVD       T+ ++ D  E T    LW M    
Sbjct: 248 RRDRLTRAIMEEHTLARQKSSGAKQHFVD----ALLTLKDQYDLSEDTIIGLLWDMITAG 307

Query: 315 --------EWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKETMRL 374
                   EWA+AE++ +P +Q+K+++E D V+G    +TE D   LPYLQ V+KE+ RL
Sbjct: 308 MDTTAITAEWAMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRL 367

Query: 375 RMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPERFLEEEAD 434
               PL++PH +  D K+ GYDIP  S + VN W +A +P  W  P EFRPERFLEE  D
Sbjct: 368 HPPTPLMLPHRSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEE--D 427

Query: 435 VEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPG--QSKIDTTEKP 454
           V+  G+DFR LPFGAGRR CPG  L + +V   +  L+ +F   PP G    +ID +E P
Sbjct: 428 VDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENP 479

[Close]

BLAST of Cp4.1LG00g04370 vs. TAIR10
Match: AT4G13310.1 (AT4G13310.1 cytochrome P450, family 71, subfamily A, polypeptide 20)

HSP 1 Score: 227.6 bits (579), Expect = 1.6e-59
Identity = 151/490 (30.82%), Postives = 253/490 (51.63%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           M+++L+   L  L   ++ +I + +   K F LPP P  +P+ GN  Q+     HR+L  
Sbjct: 1   MEMILITLCLTTLLALLLKSI-LKRTATKNFNLPPSPWRLPVIGNLHQLSLH-THRSLRS 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           ++ ++G ++LL  G+  +++VSS ++A +V+ T  +   +R +  V D     G+D+ F 
Sbjct: 61  LSLRYGPLMLLHFGRTPVLIVSSADVAHDVMKTHDLVCANRPKTKVVDKILSGGRDVAFA 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDL-KNDPKASTSGVVLRRRLQ 180
            YGE+WR+M+ I       NK+V+      EEE  R++E L K    +S S V L + L 
Sbjct: 121 PYGEYWRQMKSICIQNLLNNKMVRSYEKIREEEIKRMIEKLEKASCSSSPSPVNLSQILM 180

Query: 181 LLMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPF--LRG 240
            L  + + R+   R++   +D +     + N  R+  +   E+  G++IP L     +RG
Sbjct: 181 TLTNDIICRVALGRKYSGKKDGI----DVENIVRTFAALLGEFPVGEYIPSLSWIDRIRG 240

Query: 241 YLNVCKDVKERRLKLFKDNFVDERR------------RLTSTMSNKEDTIE--------- 300
            L+   +V ++R   F +  V E              +L +  S+K    E         
Sbjct: 241 -LDHKMEVVDKRFDEFLERVVKEHEEADKETRSDLVDKLLTIQSDKTGQFELEKSALKLI 300

Query: 301 ----------TTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQA 360
                     TTL  +EWA+ EL+ +P + KKL++E+ S     + +TE +   + YLQA
Sbjct: 301 IWDMFLAGTATTLSFLEWAMTELMRNPKVMKKLQEEIRSSSPQDLFVTEKEAEKMNYLQA 360

Query: 361 VIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHW-NKPHEFRP 420
           VIKE +RLR   PLLVP +   D KL GY+IPA ++++VNAW +  +   W     EF+P
Sbjct: 361 VIKEALRLRPPAPLLVPRVLSEDVKLKGYNIPAGTQVIVNAWAIQRDTTTWGTDAEEFKP 420

Query: 421 ERFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQ--NFELLPPPGQ 454
           ER L+   D +  G DF+++PFG+G+R CPGI     ++ +TL  +++  N+ +   P +
Sbjct: 421 ERHLDTNLDFQ--GQDFKFIPFGSGKRICPGIGFTSALIGVTLANIVKRFNWRMDVEPQR 480

[Close]

BLAST of Cp4.1LG00g04370 vs. TAIR10
Match: AT1G74550.1 (AT1G74550.1 cytochrome P450, family 98, subfamily A, polypeptide 9)

HSP 1 Score: 226.9 bits (577), Expect = 2.6e-59
Identity = 153/482 (31.74%), Postives = 248/482 (51.45%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+          +++A  +  LR +   +PPGP    + GN  Q+   L  ++ +E
Sbjct: 1   MDLLLIS------LTTIIIAAYMQNLRRRGSNIPPGPPTRFLVGNLHQL-KPLWTQSFSE 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            ++ +G I+ + +G +  VVVSS +LAK+VL  +  +  +R R       T  G D++++
Sbjct: 61  WSQTYGPIISVWLGSQLAVVVSSSDLAKQVLRDKDYQLCNRHRTARM---TQNGSDLIWS 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSG-VVLRRRLQ 180
            YG H+ KMR++ T+  F+ K ++  R   E E S +V+ + ND  +     VVLR  L 
Sbjct: 121 DYGAHYVKMRKLCTLELFSLKSIECFRSMREMEVSSMVKSIFNDFMSDDQKPVVLRNYLD 180

Query: 181 LLMYNNMYRIMFDRRFDSMEDPLFNKL-----KLINAER--------SRLSQSFEYNYGD 240
            +  N + R++  + F+  +   F  +     +L  A +         RLS  F  +   
Sbjct: 181 SVALNIVSRLVIGKTFEPKDGREFRSIVERETRLPGATKMLDYTVWLKRLSSWFTSDKAF 240

Query: 241 FIPILRP---FLRGYLNVCKDVKERRLKLFKDNFVDERRRLTSTMSNKEDTIETTLWSM- 300
              + R    F R  ++     ++++       FV     L       E+T+   +W+M 
Sbjct: 241 MKHMARKRNWFKRAVMDEVYGGRDQKC------FVQSLLELKEKDELTEETVMGLVWNML 300

Query: 301 -----------EWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKET 360
                      EWA+AE++  P +++K++DELDSV+G G  +++ DI  LP+LQ V+KE 
Sbjct: 301 TAGADTTAITIEWAMAEMIRCPTVKEKVQDELDSVVGSGRLMSDADIPKLPFLQCVLKEA 360

Query: 361 MRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPERFLEE 420
           +RL    PL++PH      ++ GY +P  + + VN   +A +P +W+ P EFRPERFL E
Sbjct: 361 LRLHPPTPLMLPHKASESVQVGGYKVPKGATVYVNVQAIARDPANWSNPDEFRPERFLVE 420

Query: 421 EADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKIDTTEK 454
           E DV+  G DFR LPFG+GRR CP   L+L ++ + LG L+  F       +  ID TEK
Sbjct: 421 ETDVK--GQDFRVLPFGSGRRVCPAAQLSLNMMTLALGSLLHCFSWTSSTPREHIDMTEK 464

[Close]

BLAST of Cp4.1LG00g04370 vs. TAIR10
Match: AT4G13290.1 (AT4G13290.1 cytochrome P450, family 71, subfamily A, polypeptide 19)

HSP 1 Score: 226.9 bits (577), Expect = 2.6e-59
Identity = 142/481 (29.52%), Postives = 252/481 (52.39%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           M+++L+   L  L   +++   + +       LPP P  +P+ GN  Q+  +  HR+L  
Sbjct: 1   MEIILVTLCLTTLLALLLLKSILKRTTTNNLNLPPSPWRLPVIGNLHQLSLN-THRSLRS 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           ++ ++G ++LL  G+  +++VSS ++A ++L T  V   +R +  V D     G+D+ F 
Sbjct: 61  LSLRYGPLMLLHFGRTPVLIVSSADVAHDILKTYDVICANRPKTKVIDKILRGGRDVAFA 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
            YGE+W++M+ I      +NK+V+  +   E+E   ++E ++N    S    V   +L +
Sbjct: 121 PYGEYWKQMKSICIQNLLSNKMVRSYKKIREDEIKLMIEKVENASSCSPPSPVNLSQLFM 180

Query: 181 LMYNNMY-RIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPF--LRG 240
            + N++  R    R++ S ED +     + N  R+  +   E+  G++IP L     +RG
Sbjct: 181 TLTNDIICRAALGRKYSSKEDGI----DVENIVRAFSALVGEFPIGEYIPSLSWIDKIRG 240

Query: 241 YLNVCKDVK-------ERRLKLFKDNFVDERRRLTSTM---SNKEDTIETTLWSM----- 300
             +  ++V        ER +K  +D   D R  L  T+    + +  ++  +W M     
Sbjct: 241 QDHKMEEVDKRFDEFLERVVKEHEDANKDTRSDLVDTLLTIQSDKSALKLIIWDMFLAGT 300

Query: 301 -------EWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAVIKETMRLR 360
                  EWA+ EL+ +P + KKL++E+ S    G+ +TE +   + YLQAVIKE +RLR
Sbjct: 301 ATSLSFLEWAMTELMRNPKVMKKLQEEIRSSSRQGLFVTEKEAEKMDYLQAVIKEALRLR 360

Query: 361 MAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWN-KPHEFRPERFLEEEAD 420
              PL+VP +   D  L GY+IPA +++++NAW +  +   W     EFRPER L+   D
Sbjct: 361 PPAPLMVPRVFSEDVTLKGYNIPAGTQVIINAWAIQRDTTTWGIDAEEFRPERHLDSILD 420

Query: 421 VEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQ--NFELLPPPGQSKIDTTEKP 454
            +  G DF+++PFG+G+R CPGI     ++ +TL  +++  N+ +   P + + D TE  
Sbjct: 421 FQ--GQDFKFIPFGSGKRICPGIGFTSALIGVTLANIVKRFNWRMDVEPQRVQHDLTEAT 474

[Close]

BLAST of Cp4.1LG00g04370 vs. NCBI nr
Match: gi|659114910|ref|XP_008457287.1| (PREDICTED: trans-cinnamate 4-monooxygenase-like [Cucumis melo])

HSP 1 Score: 834.7 bits (2155), Expect = 7.9e-239
Identity = 417/504 (82.74%), Postives = 445/504 (88.29%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEKAL+G+FV +VVAI+ISK++GKRF LPPGPIPIPIFGNWLQVGDDLNH NL E
Sbjct: 1   MDLLLLEKALVGMFVTIVVAISISKIKGKRFNLPPGPIPIPIFGNWLQVGDDLNHHNLRE 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT
Sbjct: 61  LAKRFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEA+ VVEDL+N+ +AST+GVVLR+RLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEAAIVVEDLRNNLEASTTGVVLRKRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYR+MFD+RFD+MEDPLFNKLKLINAERSRLSQSF+YNYGDFIPILRPFL  YLN
Sbjct: 181 LMYNNMYRMMFDKRFDNMEDPLFNKLKLINAERSRLSQSFDYNYGDFIPILRPFLTRYLN 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTS--------------------TMSNKE-------- 300
            CKDVKERRL+LFKD+FVDERRRLT                        N E        
Sbjct: 241 NCKDVKERRLRLFKDHFVDERRRLTGTMSKKKLVGGVKCAIDHIVEAQENGEITEDNVLF 300

Query: 301 -------DTIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQ 360
                    IETTLWSMEWAIAELVNHPNIQ K++DELDSVL  GVQITEPDIRNLPYLQ
Sbjct: 301 IVENINVAAIETTLWSMEWAIAELVNHPNIQSKVRDELDSVLKDGVQITEPDIRNLPYLQ 360

Query: 361 AVIKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRP 420
           AVIKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNPDHWNKP EFRP
Sbjct: 361 AVIKETLRLRMAIPLLVPHMNLQDAKLGGYDIPAESKILVNAWWLANNPDHWNKPQEFRP 420

Query: 421 ERFLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSK 470
           ERFLEEE +VEPSGNDFRYLPFGAGRRSCPGI+LALPI+AITLGRL+QNFEL+PPPGQSK
Sbjct: 421 ERFLEEEENVEPSGNDFRYLPFGAGRRSCPGIILALPILAITLGRLLQNFELMPPPGQSK 480

[Close]

BLAST of Cp4.1LG00g04370 vs. NCBI nr
Match: gi|585867810|gb|AHJ60265.1| (cinnamate 4-monooxygenase [Ipomoea purpurea])

HSP 1 Score: 793.5 bits (2048), Expect = 2.0e-226
Identity = 391/502 (77.89%), Postives = 432/502 (86.06%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLFVA++VAI +SKLRGK++KLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFVAIIVAIVVSKLRGKKYKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
            AKKFGDI +LRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  YAKKFGDIFMLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE E + VVED+KN+P+A+T+G VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYREGWENEIASVVEDVKNNPEAATTGTVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S +DPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMS----------------------NKEDT---- 300
           +CK+VKERRL+LFKD FVDER++L+ST S                      N+++     
Sbjct: 241 ICKEVKERRLQLFKDYFVDERKKLSSTKSMDTNSLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 -------IETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVN+P+IQKKL+DE+D+VLG GVQITEPD   LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNNPHIQKKLRDEIDTVLGPGVQITEPDTHKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE  VE +GNDFRYLPFGAGRRSCPGI+LALPI+ I LGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEKHVEANGNDFRYLPFGAGRRSCPGIILALPILGIVLGRLVQNFELLPPPGQSKVD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. NCBI nr
Match: gi|360047692|gb|AEV93475.1| (4-coumarate:CoA ligase [Caragana korshinskii])

HSP 1 Score: 793.1 bits (2047), Expect = 2.6e-226
Identity = 386/502 (76.89%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLL+EK LL LF+A  +AITISKLRGKRFKLPPGPIP+PIFGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLIEKTLLALFIAATIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AK+FG+ILLLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKRFGEILLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VV+D++N+P+A+  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRNNPEAAAGGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           LMYNNMYRIMFDRRF+S EDPLF KLK++N ERSRL+QSFEYNYGDFIPILRP L+GYL 
Sbjct: 181 LMYNNMYRIMFDRRFESEEDPLFQKLKVLNGERSRLAQSFEYNYGDFIPILRPLLKGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VK+RRL+LFKD FVDER++L ST S   +                           
Sbjct: 241 ICKEVKDRRLQLFKDYFVDERKKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKK++DE+DSVLG G Q+TEPDI+NLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDSVLGPGHQVTEPDIQNLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEE+ VE +GNDFRYLPFG GRRSCPGI+LALPI+ IT+GRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. NCBI nr
Match: gi|326366169|gb|ADZ54778.1| (trans-cinnamate 4-monooxygenase [Prunus avium])

HSP 1 Score: 792.3 bits (2045), Expect = 4.5e-226
Identity = 389/502 (77.49%), Postives = 430/502 (85.66%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF+A++VAI ISKLRGKRFKLPPGPIP+PIFGNW+QVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFIAIIVAIMISKLRGKRFKLPPGPIPVPIFGNWIQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFT +GQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ RLGWE EA+ VVED+K  P ++T+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRLGWESEAAAVVEDVKKHPGSATNGMVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRF+S EDPLF KL+ +N ERSRL+QSF+YNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFMKLRGLNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +C++VKE+R++LFKD FVDER++L+ST +   +                           
Sbjct: 241 ICEEVKEKRIRLFKDYFVDERKKLSSTKTTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQKKL+DELDSVLG GVQITEPD + LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRDELDSVLGPGVQITEPDTQKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP HW KP EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLNDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FLEEEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPPPGQSK+D
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

[Close]

BLAST of Cp4.1LG00g04370 vs. NCBI nr
Match: gi|703091683|ref|XP_010094425.1| (Trans-cinnamate 4-monooxygenase [Morus notabilis])

HSP 1 Score: 792.0 bits (2044), Expect = 5.8e-226
Identity = 390/502 (77.69%), Postives = 426/502 (84.86%), Query Frame = 1


Query: 1   MDLLLLEKALLGLFVAMVVAITISKLRGKRFKLPPGPIPIPIFGNWLQVGDDLNHRNLTE 60
           MDLLLLEK LLGLF A++VAI ISKLRGKRFKLPPGPIP+P+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLLGLFAAVIVAIVISKLRGKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  MAKKFGDILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTDKGQDMVFT 120
           +AKKFGDI LLRMGQRNLV+VSSPELAKEVLHTQGVEFGSRTRNVVFDIFT KGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVIVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNRLGWEEEASRVVEDLKNDPKASTSGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R GWE EA+ VVED+K +P+A+ +G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAASVVEDVKKNPEAARNGIVLRRRLQL 180

Query: 181 LMYNNMYRIMFDRRFDSMEDPLFNKLKLINAERSRLSQSFEYNYGDFIPILRPFLRGYLN 240
           +MYNNMYRIMFDRRFDS EDPLFNKLK +N ERSRL+QSFEYNYGDFIPILRPFLRGYL 
Sbjct: 181 MMYNNMYRIMFDRRFDSEEDPLFNKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 VCKDVKERRLKLFKDNFVDERRRLTSTMSNKED--------------------------- 300
           +CK+VKE RLKLFKD FVDER++L+S  S   +                           
Sbjct: 241 ICKEVKENRLKLFKDYFVDERKKLSSPKSTDNEGLKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 ------TIETTLWSMEWAIAELVNHPNIQKKLKDELDSVLGHGVQITEPDIRNLPYLQAV 360
                  IETTLWS+EW IAELVNHP IQ+KL+DELD+VLG GVQITEPD+  LPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRDELDTVLGRGVQITEPDMLKLPYLQAV 360

Query: 361 IKETMRLRMAIPLLVPHMNLLDAKLAGYDIPAESKILVNAWWLANNPDHWNKPHEFRPER 420
           IKET+RLRMAIPLLVPHMNL DAKL GYDIPAESKILVNAWWLANNP +W  P EFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEEADVEPSGNDFRYLPFGAGRRSCPGIVLALPIVAITLGRLIQNFELLPPPGQSKID 470
           FL+EEA VE +GNDFRYLPFG GRRSCPGI+LALPI+ ITLGRL+QNFELLPP GQSKID
Sbjct: 421 FLQEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPAGQSKID 480

The following BLAST results are available for this feature:

BLAST of Cp4.1LG00g04370 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TCMO_POPTM	9.0e-227	77.89	Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1	[more]
TCMO_POPKI	5.8e-226	77.29	Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1	[more]
TCMO_GLYEC	1.3e-225	76.49	Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1	[more]
TCMO_CATRO	4.2e-224	76.69	Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1	[more]
TCMO_SOYBN	5.5e-224	75.75	Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1	[more]

BLAST of Cp4.1LG00g04370 vs. TrEMBL


Match Name	E-value	Identity	Description
X2D8D0_IPOPU	1.4e-226	77.89	Cinnamate 4-monooxygenase OS=Ipomoea purpurea GN=C4H PE=2 SV=1	[more]
V9I5V9_9FABA	1.8e-226	76.89	4-coumarate:CoA ligase OS=Caragana korshinskii GN=C4H PE=2 SV=1	[more]
F2VR43_PRUAV	3.1e-226	77.49	Trans-cinnamate 4-monooxygenase OS=Prunus avium GN=C4H1 PE=2 SV=1	[more]
W9RIS7_9ROSA	4.1e-226	77.69	Trans-cinnamate 4-monooxygenase OS=Morus notabilis GN=L484_018794 PE=3 SV=1	[more]
B5KY03_9ROSA	5.3e-226	76.10	Cinnamate-4-hydroxylase OS=Rubus coreanus PE=2 SV=1	[more]

BLAST of Cp4.1LG00g04370 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G30490.1	1.6e-218	73.51	cinnamate-4-hydroxylase	[more]
AT2G40890.1	3.0e-71	36.17	cytochrome P450, family 98, subfamily A, polypeptide 3	[more]
AT4G13310.1	1.6e-59	30.82	cytochrome P450, family 71, subfamily A, polypeptide 20	[more]
AT1G74550.1	2.6e-59	31.74	cytochrome P450, family 98, subfamily A, polypeptide 9	[more]
AT4G13290.1	2.6e-59	29.52	cytochrome P450, family 71, subfamily A, polypeptide 19	[more]

BLAST of Cp4.1LG00g04370 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659114910\|ref\|XP_008457287.1\|	7.9e-239	82.74	PREDICTED: trans-cinnamate 4-monooxygenase-like [Cucumis melo]	[more]
gi\|585867810\|gb\|AHJ60265.1\|	2.0e-226	77.89	cinnamate 4-monooxygenase [Ipomoea purpurea]	[more]
gi\|360047692\|gb\|AEV93475.1\|	2.6e-226	76.89	4-coumarate:CoA ligase [Caragana korshinskii]	[more]
gi\|326366169\|gb\|ADZ54778.1\|	4.5e-226	77.49	trans-cinnamate 4-monooxygenase [Prunus avium]	[more]
gi\|703091683\|ref\|XP_010094425.1\|	5.8e-226	77.69	Trans-cinnamate 4-monooxygenase [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR017972	Cyt_P450_CS
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009805	coumarin biosynthetic process
biological_process	GO:0009809	lignin biosynthetic process
biological_process	GO:0006558	L-phenylalanine metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009811	stilbene biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016710	trans-cinnamate 4-monooxygenase activity
molecular_function	GO:0003779	actin binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG00g04370.1	Cp4.1LG00g04370.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 273..290 score: 3.3E-6coord: 326..337 score: 3.3E-6coord: 405..414 score: 3.
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 26..457 score: 2.4E
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 34..271 score: 1.9E-40coord: 275..462 score: 2.2
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 34..453 score: 4.45E
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 414..437 score: 4.6E-48coord: 86..107 score: 4.6E-48coord: 325..343 score: 4.6E-48coord: 366..390 score: 4.6E-48coord: 282..308 score: 4.6E-48coord: 62..81 score: 4.6E-48coord: 404..414 score: 4.6
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 407..416 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 2..469 score:
None	No IPR available	PANTHER	PTHR24298:SF56	TRANS-CINNAMATE 4-MONOOXYGENASE	coord: 2..469 score:

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG00g04370	CmaCh01G007350	Cucurbita maxima (Rimu)	cmacpeB456
Cp4.1LG00g04370	CmoCh01G007640	Cucurbita moschata (Rifu)	cmocpeB417
Cp4.1LG00g04370	Cla008096	Watermelon (97103) v1	cpewmB010
Cp4.1LG00g04370	MELO3C020483	Melon (DHL92) v3.5.1	cpemeB006
Cp4.1LG00g04370	Lsi02G012060	Bottle gourd (USVL1VR-Ls)	cpelsiB011
Cp4.1LG00g04370	Bhi08G000346	Wax gourd	cpewgoB0014

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG00g04370	Cp4.1LG02g11240	Cucurbita pepo (Zucchini)	cpecpeB010

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG00g04370	Silver-seed gourd	carcpeB0731