CmoCh16G003360.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh16G003360.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Trypsin inhibitor 1
Location	Cmo_Chr16 : 1563756 .. 1563995 (+)
Sequence length	240

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGAGTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGGTGCCCGAGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCATATGCCGGCCCAACGGCTTCTGTGGTTGA

mRNA sequence

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGAGTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGGTGCCCGAGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCATATGCCGGCCCAACGGCTTCTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGAGTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGGTGCCCGAGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCATATGCCGGCCCAACGGCTTCTGTGGTTGA

Homology

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BLAST of CmoCh16G003360.1 vs. Swiss-Prot
Match: ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.2e-16
Identity = 41/65 (63.08%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MAAFAESAAVHGG--EVIQLVSDGVNDFPRKMMNGARGCPRILMECEVDSDCLPGCICRP 76
          MAAF ESA    G  EVIQLVSDGVN++  KMM G   CPRILM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of CmoCh16G003360.1 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-13
Identity = 37/65 (56.92%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGARG--CPRILMECEVDSDCLPGCICRP 76
          MA+ AES+ V   EVI+L+SDG ND PRK+M+G  G  CPRILM C+ D DC+  C C  
Sbjct: 1  MASVAESSGVV--EVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 63

[Close]

BLAST of CmoCh16G003360.1 vs. Swiss-Prot
Match: ITR2_ECBEL (Trypsin inhibitor 2 OS=Ecballium elaterium PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 2.9e-08
Identity = 22/28 (78.57%), Postives = 24/28 (85.71%), Query Frame = 1


Query: 52 GCPRILMECEVDSDCLPGCICRPNGFCG 80
          GCPRILM C+ DSDCL GC+C PNGFCG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of CmoCh16G003360.1 vs. Swiss-Prot
Match: ITR2_BRYDI (Trypsin inhibitor 2 OS=Bryonia dioica PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 6.4e-08
Identity = 21/29 (72.41%), Postives = 25/29 (86.21%), Query Frame = 1


Query: 51 RGCPRILMECEVDSDCLPGCICRPNGFCG 80
          RGCPRILM C+ DSDCL GC+C+ NG+CG
Sbjct: 1  RGCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of CmoCh16G003360.1 vs. Swiss-Prot
Match: ITR4_CYCPE (Trypsin inhibitor 4 OS=Cyclanthera pedata PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.2e-07
Identity = 22/29 (75.86%), Postives = 24/29 (82.76%), Query Frame = 1


Query: 51 RGCPRILMECEVDSDCLPGCICRPNGFCG 80
          R CPRILMEC+ DSDCL  CIC+ NGFCG
Sbjct: 1  RICPRILMECKADSDCLAQCICQENGFCG 29

[Close]

BLAST of CmoCh16G003360.1 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 9.5e-19
Identity = 47/81 (58.02%), Postives = 62/81 (76.54%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKM-MNGARGCPRILM 60
          MEWK+ AL+AM+G+LLMA F ES  +    E+IQLVSDGVN++ + +  + A GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECEVDSDCLPGCICRPNGFCG 80
          +C+ DSDC PGC C+P G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CmoCh16G003360.1 vs. TrEMBL
Match: A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 5.1e-12
Identity = 42/82 (51.22%), Postives = 51/82 (62.20%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMN-GARGCPRIL 60
          MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN   R CPRIL
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60

Query: 61 MECEVDSDCLPGCICRPNGFCG 80
           EC  D DC+  CIC  NGFCG
Sbjct: 61 EECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CmoCh16G003360.1 vs. TrEMBL
Match: A0A0A7HIA9_9ROSI (TIPRE5 (Fragment) OS=Momordica friesiorum GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.2e-06
Identity = 29/76 (38.16%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 10 AMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGA------RGCPRILMECEVD 69
          A+V ++L+A  A+S   +GG+ I L+S+G     + + +G       R CP+IL  C  D
Sbjct: 3  ALVAMMLVATSADS---NGGDTIHLISNGRAQTGQDINSGGVYSEEQRACPKILKRCRRD 62

Query: 70 SDCLPGCICRPNGFCG 80
          SDC   C+C+ NG+CG
Sbjct: 63 SDCPGACVCQDNGYCG 75

[Close]

BLAST of CmoCh16G003360.1 vs. TrEMBL
Match: A0A0A7HF87_9ROSI (TIPTOP4 protein OS=Momordica subangulata GN=TIPTOP4 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.2e-06
Identity = 32/79 (40.51%), Postives = 44/79 (55.70%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGARGCPRILMEC 60
          ME K+  L+ +V ++L+A    S   + G+   L+SDG        +NG   CP+IL  C
Sbjct: 1  MESKKILLVVLVAMMLVAT---STCFNDGDTTDLISDGRAQMD---INGG-ACPKILQRC 60

Query: 61 EVDSDCLPGCICRPNGFCG 80
            DSDC   CICR NG+CG
Sbjct: 61 RRDSDCPSACICRGNGYCG 72

[Close]

BLAST of CmoCh16G003360.1 vs. TrEMBL
Match: A0A0A7HG47_9ROSI (TIPRE1 (Fragment) OS=Momordica anigosantha GN=TIPRE1 PE=3 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 7.1e-06
Identity = 28/72 (38.89%), Postives = 40/72 (55.56%), Query Frame = 1


Query: 14 LLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGA------RGCPRILMECEVDSDCL 73
          L+ M   A SA  +GG+ I L+S+G     + + +G       R CP+IL  C  DSDC 
Sbjct: 4  LVAMMLVATSADFNGGDTIHLISNGRAQTGQDINSGGVYSEEQRACPKILKRCRRDSDCP 63

Query: 74 PGCICRPNGFCG 80
            C+C+ NG+CG
Sbjct: 64 GACVCQDNGYCG 75

[Close]

BLAST of CmoCh16G003360.1 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 100.5 bits (249), Expect = 1.4e-18
Identity = 47/81 (58.02%), Postives = 62/81 (76.54%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFAESAAVH-GGEVIQLVSDGVNDFPRKM-MNGARGCPRILM 60
          MEWK+ AL+AM+G+LLMA F ES  +    E+IQLVSDGVN++ + +  + A GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECEVDSDCLPGCICRPNGFCG 80
          +C+ DSDC PGC C+P G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CmoCh16G003360.1 vs. NCBI nr
Match: gi|8134511|sp|Q43667.1|ITR1_TRIKI (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor)

HSP 1 Score: 85.9 bits (211), Expect = 3.5e-14
Identity = 41/65 (63.08%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MAAFAESAAVHGG--EVIQLVSDGVNDFPRKMMNGARGCPRILMECEVDSDCLPGCICRP 76
          MAAF ESA    G  EVIQLVSDGVN++  KMM G   CPRILM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of CmoCh16G003360.1 vs. NCBI nr
Match: gi|778697386|ref|XP_011654311.1| (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 7.3e-12
Identity = 42/82 (51.22%), Postives = 51/82 (62.20%), Query Frame = 1


Query: 1   MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMN-GARGCPRIL 60
           MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN   R CPRIL
Sbjct: 126 MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 185

Query: 61  MECEVDSDCLPGCICRPNGFCG 80
            EC  D DC+  CIC  NGFCG
Sbjct: 186 EECTTDDDCMNDCICLSNGFCG 204

[Close]

BLAST of CmoCh16G003360.1 vs. NCBI nr
Match: gi|700197602|gb|KGN52760.1| (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 7.3e-12
Identity = 42/82 (51.22%), Postives = 51/82 (62.20%), Query Frame = 1


Query: 1  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMN-GARGCPRIL 60
          MEWK+  ++A+VG+L+MAA  E   AA+    V  L     N+ PRKMMN   R CPRIL
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGL---NKNELPRKMMNEETRMCPRIL 60

Query: 61 MECEVDSDCLPGCICRPNGFCG 80
           EC  D DC+  CIC  NGFCG
Sbjct: 61 EECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CmoCh16G003360.1 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 76.6 bits (187), Expect = 2.1e-11
Identity = 37/65 (56.92%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGARG--CPRILMECEVDSDCLPGCICRP 76
          MA+ AES+ V   EVI+L+SDG ND PRK+M+G  G  CPRILM C+ D DC+  C C  
Sbjct: 1  MASVAESSGVV--EVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 63

The following BLAST results are available for this feature:

BLAST of CmoCh16G003360.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR1_TRIKI	2.2e-16	63.08	Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1	[more]
ITR5_LUFAE	1.3e-13	56.92	Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR2_ECBEL	2.9e-08	78.57	Trypsin inhibitor 2 OS=Ecballium elaterium PE=1 SV=2	[more]
ITR2_BRYDI	6.4e-08	72.41	Trypsin inhibitor 2 OS=Bryonia dioica PE=1 SV=1	[more]
ITR4_CYCPE	3.2e-07	75.86	Trypsin inhibitor 4 OS=Cyclanthera pedata PE=1 SV=1	[more]

BLAST of CmoCh16G003360.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY34_CUCSA	9.5e-19	58.02	Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1	[more]
A0A0A0KT01_CUCSA	5.1e-12	51.22	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1	[more]
A0A0A7HIA9_9ROSI	3.2e-06	38.16	TIPRE5 (Fragment) OS=Momordica friesiorum GN=TIPRE5 PE=3 SV=1	[more]
A0A0A7HF87_9ROSI	3.2e-06	40.51	TIPTOP4 protein OS=Momordica subangulata GN=TIPTOP4 PE=3 SV=1	[more]
A0A0A7HG47_9ROSI	7.1e-06	38.89	TIPRE1 (Fragment) OS=Momordica anigosantha GN=TIPRE1 PE=3 SV=1	[more]

BLAST of CmoCh16G003360.1 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh16G003360.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197603\|gb\|KGN52761.1\|	1.4e-18	58.02	Trypsin inhibitor 1 [Cucumis sativus]	[more]
gi\|8134511\|sp\|Q43667.1\|ITR1_TRIKI	3.5e-14	63.08	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]
gi\|778697386\|ref\|XP_011654311.1\|	7.3e-12	51.22	PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
gi\|700197602\|gb\|KGN52760.1\|	7.3e-12	51.22	hypothetical protein Csa_4G000805 [Cucumis sativus]	[more]
gi\|462426\|sp\|P34950.1\|ITR5_LUFAE	2.1e-11	56.92	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000737	Prot_inh_squash
IPR011052	Proteinase_amylase_inhib_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh16G003360	CmoCh16G003360	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003360.1	CmoCh16G003360.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003360.1.exon.1	CmoCh16G003360.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh16G003360.1.CDS.1	CmoCh16G003360.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 51..79 score: 5.3
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 53..72 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 50..79 score: 5.02
None	No IPR available	SMART	SM00286	PTI_1	coord: 51..79 score: 2.4