CmoCh14G020150.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh14G020150.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Heavy metal transport/detoxification superfamily protein
Location	Cmo_Chr14 : 14755018 .. 14757404 (+)
Sequence length	732

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAGAAAGCTGAAGCCATTACTGCCATTTACAAAGTAAACCTTCATTGCCAACAATGTTGGAGAGAGATCAAAAGGCCTCTTGCTAGAACACAAGGTAAAGTTCACTATTTTCTTCATGGGTTTCAAATTTCTTTGGTTTTTTCGTTCATTTTCTTGCCAACCAAACACAATTATGTTCGATGATGAAAGTCCCACCTCAGCTAATTTAGAGAATGATCATGGATTTATAACCAAATAATACTATCTCTATTAGTACGAGGTTTTCTCGAAATGATCATGGATTTATAATCAAATAATACTATCTCTATTGGTACGAGGCCTTTTGAGGAGCACAAAGTAAAACTATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTATAAATAGTCGTGTTTACAAAAATAAAAATAAAATAAAAAGACATTTTTTTGTTCATCTCCAAAAAAACGATCCCCACTCCTCTCTCCACTTTGATCGATCTACTCCGATATGGAACAACTCATTCATGTTAAAAATAAAAAAATAAATTATTATTAAATTACAAGTTTAGTCCCTAAACTTTTTTTTTTCTTTTTTTAAACTGCTTCAACTAAGGCTCATGAACTTAATGTCTTATAAGTCTCTGAACTCTAAAAATATATAATTTGTAAACTATCAATTTTTTAAAATATATTGGATAGATCAAACGCCTATTAACCACAAAATTGAAAGTTCAATAATCTATTCGACGTTTTTTAAAGTTCAAGAATCAAATAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCGACTTTAATTTAATTATTAACCTTTTTTTTAATTTCATATTAGGGATTCATAATGTTGAAGTTGATATGGAGAAAAATGAAATTAGGGTGAAAGGTTCCAATTTGGATGTGCTCAAAATTCACAAGCAAATAGAGAAATTGAGCAAGAAAAGGGTTGACTTAATTTCTCCCAAAGTCAAGCCCAAGGAGAAAGAAACTCCTAAACCCAACGACAACAAACCCAAGCCAAGCCTCGTGAGTATAATTTCATTATTTATCAATTAAATATTTATTTACATATTTATTACATCATAAATATATTTAGTATATATATATATGTTAAATTAAATTAAATATATTATTTTCCACTTGGCAGATTCAACGCACAATAACAGCTAAGGTTCACTTGCACTGCGCCAAATGCGAGAAAGACCTCAAGAACAATTTGCTAAAGCATAAAGGTCTTTTATTTTATTTTTCATCTCTATATGTTATTCAAACCATTTTTAAGTTAGAAAATTCAAAAAAAAAAAAAAAAAAAAATCTCTATTTTAATTTGAACCCTAAACTTTTAAATATTGTGATTTAGTCCTCAAATTTCGTCTTTTGATCCGTTTATAAAATTAGAAAATAAATTCTACTCAATTTTTTATAATAAATCTTAAAATAATTTTAAAAATTCAAGGACTAAAATGAAATTTTGTAATGTTTAAGATCTAATTTAAAATGGACAAATTTAATAATTATTTTAAACTATTTTTAAATGTTTTTTTTTCTTTTTGTCTAATTTAAACCTAATTTAATAAATTTAATAATATGTCTAATTTTGAGATTCATGACAAAGTTGGGATTAAAATTAAATTTTTAAAAAGTATATCGACTAAAATAGAATAAATTTAAAATACGAGGACCATAATGATATTTTAACGAACAAAAATACTATTTTTTTAAATTATAAAAACAGCCCAAAAAGTTATGAAAAACACAAAAACTTCCCATTTGAAGGACAAATAATTTGAATATTTGCAGGAATATATAATGTGAAAACAGACATGAAAGCTCAAACCCTAACAATGGAAGGAACCATAGAACCAGAGAAGTTCAAGTCTTATCTGAAGAAGAAGCTGCAGAAACACGCTGACATTACTACGGCGGTCGATTCATCGAAGCCACCGGAGAAGAAGAAAGAAAGTAGTGAGCAAAAAGAGAAGCCTAAAGAAAAATCTTCATCCGAGACGACGACGACGACAACGACCAAGATAGTTGCAGAGATTCAAACCAAAGATACCGATAATAATAAAAATAACGCTCCTTATTTCGTTCACTACGTTTACGCTCCTCAATTGTTTAGCGACGAAAATCCAAATGCATGTCAAGTAATGTAA

mRNA sequence

ATGGGAGAGAAAGCTGAAGCCATTACTGCCATTTACAAAGTAAACCTTCATTGCCAACAATGTTGGAGAGAGATCAAAAGGCCTCTTGCTAGAACACAAGGGATTCATAATGTTGAAGTTGATATGGAGAAAAATGAAATTAGGGTGAAAGGTTCCAATTTGGATGTGCTCAAAATTCACAAGCAAATAGAGAAATTGAGCAAGAAAAGGGTTGACTTAATTTCTCCCAAAGTCAAGCCCAAGGAGAAAGAAACTCCTAAACCCAACGACAACAAACCCAAGCCAAGCCTCATTCAACGCACAATAACAGCTAAGGTTCACTTGCACTGCGCCAAATGCGAGAAAGACCTCAAGAACAATTTGCTAAAGCATAAAGGAATATATAATGTGAAAACAGACATGAAAGCTCAAACCCTAACAATGGAAGGAACCATAGAACCAGAGAAGTTCAAGTCTTATCTGAAGAAGAAGCTGCAGAAACACGCTGACATTACTACGGCGGTCGATTCATCGAAGCCACCGGAGAAGAAGAAAGAAAGTAGTGAGCAAAAAGAGAAGCCTAAAGAAAAATCTTCATCCGAGACGACGACGACGACAACGACCAAGATAGTTGCAGAGATTCAAACCAAAGATACCGATAATAATAAAAATAACGCTCCTTATTTCGTTCACTACGTTTACGCTCCTCAATTGTTTAGCGACGAAAATCCAAATGCATGTCAAGTAATGTAA

Coding sequence (CDS)

ATGGGAGAGAAAGCTGAAGCCATTACTGCCATTTACAAAGTAAACCTTCATTGCCAACAATGTTGGAGAGAGATCAAAAGGCCTCTTGCTAGAACACAAGGGATTCATAATGTTGAAGTTGATATGGAGAAAAATGAAATTAGGGTGAAAGGTTCCAATTTGGATGTGCTCAAAATTCACAAGCAAATAGAGAAATTGAGCAAGAAAAGGGTTGACTTAATTTCTCCCAAAGTCAAGCCCAAGGAGAAAGAAACTCCTAAACCCAACGACAACAAACCCAAGCCAAGCCTCATTCAACGCACAATAACAGCTAAGGTTCACTTGCACTGCGCCAAATGCGAGAAAGACCTCAAGAACAATTTGCTAAAGCATAAAGGAATATATAATGTGAAAACAGACATGAAAGCTCAAACCCTAACAATGGAAGGAACCATAGAACCAGAGAAGTTCAAGTCTTATCTGAAGAAGAAGCTGCAGAAACACGCTGACATTACTACGGCGGTCGATTCATCGAAGCCACCGGAGAAGAAGAAAGAAAGTAGTGAGCAAAAAGAGAAGCCTAAAGAAAAATCTTCATCCGAGACGACGACGACGACAACGACCAAGATAGTTGCAGAGATTCAAACCAAAGATACCGATAATAATAAAAATAACGCTCCTTATTTCGTTCACTACGTTTACGCTCCTCAATTGTTTAGCGACGAAAATCCAAATGCATGTCAAGTAATGTAA

Homology

[Close]

BLAST of CmoCh14G020150.1 vs. Swiss-Prot
Match: HIP3_ARATH (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana GN=HIPP3 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.7e-17
Identity = 76/268 (28.36%), Postives = 131/268 (48.88%), Query Frame = 1


Query: 7   AITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQIEKL 66
           +IT + KV++HC+ C   I + +   QG+  V+ +    ++ V G+ LD +K+ +++E+ 
Sbjct: 25  SITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA-LDPVKLREKLEEK 84

Query: 67  SKKRVDLISP-----------------KVKPKEKETPKPNDNKPKPSLIQRTITAKVHLH 126
           +KK+VDL+SP                 K K +EK+ P  ND KPK + +  T   K++ H
Sbjct: 85  TKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETPVT-TAVLKLNFH 144

Query: 127 CAKCEKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVD 186
           C  C   ++  + K KG+  +  D +   LT++GT++ +K    L +KL++  +I     
Sbjct: 145 CQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEKLKRAVEIV---- 204

Query: 187 SSKPPEKKKESSEQKEKPKEKSSSETTTTTTTKIVAEIQTKDTDNNKNNA---------- 244
              PP+K+K+     E  ++K           K   +   +  +  +  A          
Sbjct: 205 ---PPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGEGVNMMEYMAAQPAYGYGYY 264

[Close]

BLAST of CmoCh14G020150.1 vs. TrEMBL
Match: A0A0A0L4G4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G127060 PE=4 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.4e-73
Identity = 161/218 (73.85%), Postives = 182/218 (83.49%), Query Frame = 1


Query: 42  MEKNEIRVKGSNLDVLKIHKQIEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRT 101
           MEKNEIRVKGSNLDVLKI KQIEKLSKK+V+LISPKVKPKEK+ PKP D+KPKP+++ R 
Sbjct: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60

Query: 102 ITAKVHLHCAKCEKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKH 161
           ITAKVHLHC KCE+DLKN LLKHKGIY+VKTD+KAQTLTMEG+IE EKFKSYLK KLQKH
Sbjct: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120

Query: 162 ADITT---AVDSSK----PPEKKKES-SEQKEKPKEKSSSETTTTTTTK---IVAEIQTK 221
            DIT    + DSSK      EKKKES +++KEKPKEK+SSETT TTT K   +VAEIQ+K
Sbjct: 121 VDITVDTKSTDSSKSTAVASEKKKESNTDKKEKPKEKASSETTITTTDKKTIVVAEIQSK 180

Query: 222 -----DTDNNKNNAPYFVHYVYAPQLFSDENPNACQVM 244
                D +N  NN PYF+HYVYAPQLFSDENPNAC+VM
Sbjct: 181 ENNSNDINNKNNNVPYFIHYVYAPQLFSDENPNACRVM 218

[Close]

BLAST of CmoCh14G020150.1 vs. TrEMBL
Match: B9SKJ8_RICCO (Metal ion binding protein, putative OS=Ricinus communis GN=RCOM_0659680 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.2e-57
Identity = 129/242 (53.31%), Postives = 172/242 (71.07%), Query Frame = 1


Query: 6   EAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQIEK 65
           E ITA+YKVNLHCQQC R+IK+PL   QG+HNV+VD +K EI+VKG  +DV+KIHKQIEK
Sbjct: 15  EVITAVYKVNLHCQQCARDIKKPLMNMQGVHNVDVDFQKAEIKVKGV-IDVIKIHKQIEK 74

Query: 66  LSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLLKHK 125
            SKK+V+++SP++K K     +    +     I RT + KVH+HC KCE DL+N LLKH+
Sbjct: 75  WSKKKVEMVSPEIKIKNTGATEKKVVEQTKKAILRTTSIKVHMHCDKCENDLQNRLLKHE 134

Query: 126 GIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKPPEKKKESSEQKE 185
           GIY+VKT+MK QTL ++G IE +K  +Y++KK+ K+A+I T+      PEK +E  E KE
Sbjct: 135 GIYSVKTNMKTQTLLVQGIIESDKLLAYIRKKVHKNAEIITS-----KPEKMEEKKEVKE 194

Query: 186 -KPKEKSSSETTTTTTTKIVAEIQTKD---TDNNKNNAPYFVHYVYAPQLFSDENPNACQ 244
            + KEK   E  +  +TK+V E +T+        + NAPYF+HYVYAPQLFSDENPNAC 
Sbjct: 195 AEIKEKQQVEAISINSTKLV-EFKTEKKVAAQTTEGNAPYFIHYVYAPQLFSDENPNACI 249

[Close]

BLAST of CmoCh14G020150.1 vs. TrEMBL
Match: A0A061FTM9_THECC (Metal ion binding protein, putative OS=Theobroma cacao GN=TCM_011949 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.2e-57
Identity = 129/236 (54.66%), Postives = 172/236 (72.88%), Query Frame = 1


Query: 8   ITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQIEKLS 67
           ITA+YKVNLHC+QC R+IK+PL RTQG+H+V+V++EK EI+VKG  +DV+KIHK IEKLS
Sbjct: 16  ITAVYKVNLHCRQCARKIKKPLIRTQGVHSVDVEIEKGEIKVKGV-IDVIKIHKLIEKLS 75

Query: 68  KKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLLKHKGI 127
           +K+V+L+SP++K K   T        +P L   T   KVHLHC KCE+DL+  LLKHKGI
Sbjct: 76  QKKVELVSPQIKEKAAATQTKVKETQEPIL--STTLVKVHLHCDKCEQDLRKKLLKHKGI 135

Query: 128 YNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKPPEKKKESSEQKEKP 187
           Y+VKTDMKAQTLT++GTI+ +   SY++K + KHA+I ++   +K  E+KKE+ +   K 
Sbjct: 136 YSVKTDMKAQTLTVQGTIKSDDLLSYIRKNVHKHAEIVSSKTEAK--EEKKEAKKDDVKD 195

Query: 188 KEKSSSETTTTTTTKIVAEIQTKDTDNNKNNAPYFVHYVYAPQLFSDENPNACQVM 244
             K SSE T  T  K   +++ K   + + N PYFVHYVYAPQLFSDENPNAC ++
Sbjct: 196 GAK-SSEKTQVTEFKQQEKVEVK---SKEGNTPYFVHYVYAPQLFSDENPNACSIL 242

[Close]

BLAST of CmoCh14G020150.1 vs. TrEMBL
Match: V4MQL6_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10009467mg PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.8e-55
Identity = 136/273 (49.82%), Postives = 177/273 (64.84%), Query Frame = 1


Query: 1   MGEKAEA-------ITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSN 60
           MGEK E        ITA+YKV+LHC++C  +IK+PL R QG+H VE D+EKNEI+VKG  
Sbjct: 1   MGEKEEKKEKGEEIITAVYKVHLHCRKCACDIKKPLLRFQGVHGVEFDLEKNEIKVKGK- 60

Query: 61  LDVLKIHKQIEKLSKKRVDLISPKVKPKEKETP--------KPNDNKPKPSLIQRTITAK 120
           ++V+KIHKQIEK SKK+V+LISPK    +K T         +    + K   I+ TI  K
Sbjct: 61  IEVVKIHKQIEKWSKKKVELISPKPSEVKKTTTTTTTTTVAEKKTTEVKKDAIRTTIL-K 120

Query: 121 VHLHCAKCEKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADIT 180
           VH+HCAKC+KDL+N LLKHK I+ VKTD KAQTLT++GTIE  K  +Y++KK+ KHA+I 
Sbjct: 121 VHIHCAKCDKDLQNKLLKHKAIHIVKTDSKAQTLTVQGTIESAKLLTYIRKKVHKHAEI- 180

Query: 181 TAVDSSKPPEKKKESSEQKEKPKEKSSSE---------------TTTTTTTKIVAEIQTK 240
             + S    EKKKE  E+K+K +EK   E                T T T ++  +I+ +
Sbjct: 181 --ISSKTDEEKKKEEEEKKKKEEEKKKKEEEEKKKKEEVKVEVTKTITETVELKEKIKVE 240

Query: 241 DTDNNKNNAPYFVHYVYAPQLFSDENPNACQVM 244
              +   N PYFVHYVYAPQLFSDENPNAC ++
Sbjct: 241 GQKDKDGNIPYFVHYVYAPQLFSDENPNACCIV 268

[Close]

BLAST of CmoCh14G020150.1 vs. TrEMBL
Match: A0A059CE78_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01129 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.3e-55
Identity = 121/253 (47.83%), Postives = 174/253 (68.77%), Query Frame = 1


Query: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62
           +K + ITA+YKVNLHC +C  E++RPL RTQG+H VEV+ME NE++VKG+ +D + I+K+
Sbjct: 7   KKVDVITAVYKVNLHCPECAGEVRRPLLRTQGVHGVEVNMESNEVKVKGA-IDPVGIYKK 66

Query: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLL 122
           IE+LSK++V+LISPK+  K  E  K    +P   L  RT   KV++HC +CE+DL+  L+
Sbjct: 67  IERLSKRKVELISPKIDIKAAEIKKKVVKEPPKELPVRTHNLKVYMHCDQCERDLRRKLI 126

Query: 123 KHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKPPEKKKESSE 182
           +H+GIY+VKTD+KAQT+T++GTIEPEK  SY+++K+ K A++       K  +K+++  E
Sbjct: 127 RHRGIYSVKTDIKAQTMTVQGTIEPEKLLSYVRRKVHKQAELIPPKPEKKEEKKEEKKGE 186

Query: 183 QKEKPK------------EKSSSETTTTTTTKIVAEIQTKDTDNNKNNAPYFVHYVYAPQ 242
           +KE+ K            E    ET   T  K V E+++K+ +      PYF+HYVYAPQ
Sbjct: 187 KKEEEKKVTIEKEKVKVVETKGKETVEFTEEKKVVEVKSKEGN------PYFIHYVYAPQ 246

Query: 243 LFSDENPNACQVM 244
           LFSDENPN+C VM
Sbjct: 247 LFSDENPNSCSVM 252

[Close]

BLAST of CmoCh14G020150.1 vs. TAIR10
Match: AT1G29000.1 (AT1G29000.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 9.2e-56
Identity = 128/276 (46.38%), Postives = 173/276 (62.68%), Query Frame = 1


Query: 6   EAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQIEK 65
           E ITA+YKV+LHC++C  +IK+PL R QG+ NV+ D+EKNEI+VKG  ++V+KIHKQIEK
Sbjct: 13  EIITAVYKVHLHCRKCACDIKKPLLRFQGVQNVDFDLEKNEIKVKGK-IEVVKIHKQIEK 72

Query: 66  LSKKRVDLISPKVKPKEKETPKPNDN---KPKPSLIQ----RTITAKVHLHCAKCEKDLK 125
            SKK+V+LISPK    +K T         + K + I+    RT   KVH+HCA+C+KDL+
Sbjct: 73  WSKKKVELISPKPSEVKKTTTTTTTTSVVEKKTTEIKKDVIRTTVLKVHIHCAQCDKDLQ 132

Query: 126 NNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTA----------- 185
           + LLKHK I+ VKTD KAQTLT++GTIE  K  +Y+KKK+ KHA+I ++           
Sbjct: 133 HKLLKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYIKKKVHKHAEIISSKTEEEKKKEEE 192

Query: 186 -------------VDSSKPPEKKKESSEQKEKPKEKSSSETTTTTTTKIVAE-------I 244
                         D  K  E+KK+  E K+K  EK   E     TTK + +       +
Sbjct: 193 DKKKKEEEDKKKKEDEKKKEEEKKKEEENKKKEGEKKKEEVKVEVTTKTITQVVEYKEIV 252

[Close]

BLAST of CmoCh14G020150.1 vs. TAIR10
Match: AT5G50740.3 (AT5G50740.3 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.0e-30
Identity = 86/266 (32.33%), Postives = 142/266 (53.38%), Query Frame = 1


Query: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62
           +K E    + K+ +HC+ C ++I R L   +G+ +V  D + +++ VKG   D LK+ ++
Sbjct: 29  KKKEPQEIVLKIFMHCEGCAKKIHRCLKGFEGVEDVTTDCKTSKVVVKGEKADPLKVLQR 88

Query: 63  IEKLSKKRVDLISPKVKPK---------EKETPKPNDNKPKPSLIQRTITAKVHLHCAKC 122
           +++ S ++V+LISP  +PK         EKE PKP + K +      T+  +VH+HC  C
Sbjct: 89  LQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEEKKEEVV----TVVLRVHMHCEAC 148

Query: 123 EKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKP 182
             +++  +++ KG+ +V+ D KA  ++++G   PEK   ++ K++ KHA +       KP
Sbjct: 149 AMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVEFIYKRIGKHAAVVKQDPPPKP 208

Query: 183 PEKKKESSEQKEKPKE-------KSSSETTTTTTTK---IVAEIQTKDTDNNKNNAPY-- 242
           PEK+KE+ ++ EK KE       K + E       K     AE   K  D  KN   Y  
Sbjct: 209 PEKEKETKDKDEKKKEEGQPKEGKEAKENGGGGGAKGDGAAAEEGNKVVDLKKNEYQYQP 268

Query: 243 ----FVHYVYAPQLFSDENPNACQVM 244
                  + Y PQ+FSDENPNAC ++
Sbjct: 269 PRYPVEMFAYPPQIFSDENPNACTII 290

[Close]

BLAST of CmoCh14G020150.1 vs. TAIR10
Match: AT5G63530.1 (AT5G63530.1 farnesylated protein 3)

HSP 1 Score: 112.1 bits (279), Expect = 4.9e-25
Identity = 81/282 (28.72%), Postives = 140/282 (49.65%), Query Frame = 1


Query: 11  IYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQIEKLSKKR 70
           + KV +HC+ C R+++R L   +G+ +V  D +  ++ VKG   D LK+  ++++ + ++
Sbjct: 76  VLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQ 135

Query: 71  VDLISPKVKP-----KEKETPKP--NDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLLK 130
           V L+SP   P     K+ E  KP   + K +P ++  T+  KVH+HC  C  ++K  +++
Sbjct: 136 VQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVV-TVVLKVHMHCEACATEIKKRIMR 195

Query: 131 HKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKPPEKKKESS-- 190
            KG+ + ++D+K+  +T++G  EP+K   Y+ K+  KHA I   +D   PP  ++ ++  
Sbjct: 196 MKGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAI-MKIDPPPPPPPEEAAAAA 255

Query: 191 --EQKEKPKEKSSSETTTTTTTKIVAEIQTKDTDNNKNNA-------------------- 244
             E+KE+ K +  S+       K   + + K+ D  K                       
Sbjct: 256 EGEKKEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKI 315

[Close]

BLAST of CmoCh14G020150.1 vs. TAIR10
Match: AT3G02960.1 (AT3G02960.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 3.6e-20
Identity = 64/244 (26.23%), Postives = 120/244 (49.18%), Query Frame = 1


Query: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62
           +K +    + KV +HC+ C  ++   L    G+ +++ ++  N++ V G   D LKI ++
Sbjct: 31  KKNQCKEIVLKVYMHCEGCASQVSHCLRGYDGVEHIKTEIGDNKVVVSGKFDDPLKILRR 90

Query: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLL 122
           ++K   +  ++ISPK  PK+ +       +  P +  +T   ++++HC  C  ++K  + 
Sbjct: 91  VQKKFSRNAEMISPKHNPKQDQKEPQQKKESAPEI--KTAILRMNMHCEGCVHEIKRGIE 150

Query: 123 KHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKPPEKKKESSE 182
           K KGI +V+ D    T+ + G ++P K    +KKKL KHA++ + +      EK K++++
Sbjct: 151 KIKGIQSVEPDRSKSTVVVRGVMDPPKLVEKIKKKLGKHAELLSQI-----TEKGKDNNK 210

Query: 183 QKEKPKEKSSSETTTTTTTKIVAEIQTKDTDNNK---NNAPYFVHYVYAPQLFSDENPNA 242
           +    KE+S                     D NK       Y   + Y  Q+FSDEN ++
Sbjct: 211 KNNNKKEES---------------------DGNKIFSYPPQYSSQHAYPSQIFSDENVHS 246

Query: 243 CQVM 244
           C +M
Sbjct: 271 CSIM 246

[Close]

BLAST of CmoCh14G020150.1 vs. TAIR10
Match: AT5G60800.2 (AT5G60800.2 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.5e-18
Identity = 78/268 (29.10%), Postives = 133/268 (49.63%), Query Frame = 1


Query: 7   AITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQIEKL 66
           +IT + KV++HC+ C   I + +   QG+  V+ +    ++ V G+ LD +K+ +++E+ 
Sbjct: 25  SITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA-LDPVKLREKLEEK 84

Query: 67  SKKRVDLISP-----------------KVKPKEKETPKPNDNKPKPSLIQRTITAKVHLH 126
           +KK+VDL+SP                 K K +EK+ P  ND KPK + +  T   K++ H
Sbjct: 85  TKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETPV-TTAVLKLNFH 144

Query: 127 CAKCEKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVD 186
           C  C   ++  + K KG+  +  D +   LT++GT++ +K    L +KL++  +I     
Sbjct: 145 CQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEKLKRAVEIV---- 204

Query: 187 SSKPPEKKKE---SSEQKEKP---------KEKSSSETTTTTTTKI-VAEIQTKDTDNNK 244
              PP+K+K+    +E  EK          KEK+ ++        +     Q        
Sbjct: 205 ---PPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGEGVNMMEYMAAQPAYGYGYY 264

[Close]

BLAST of CmoCh14G020150.1 vs. NCBI nr
Match: gi|449433137|ref|XP_004134354.1| (PREDICTED: uncharacterized protein PF11_0207 [Cucumis sativus])

HSP 1 Score: 350.9 bits (899), Expect = 1.8e-93
Identity = 190/257 (73.93%), Postives = 216/257 (84.05%), Query Frame = 1


Query: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62
           EK E ITAIYK+NLHC QCWR+IK+PL+ TQG+ NVEVDMEKNEIRVKGSNLDVLKI KQ
Sbjct: 5   EKVEGITAIYKLNLHCHQCWRDIKKPLSTTQGVQNVEVDMEKNEIRVKGSNLDVLKIQKQ 64

Query: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLL 122
           IEKLSKK+V+LISPKVKPKEK+ PKP D+KPKP+++ R ITAKVHLHC KCE+DLKN LL
Sbjct: 65  IEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRIITAKVHLHCPKCEQDLKNKLL 124

Query: 123 KHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITT---AVDSSK----PPE 182
           KHKGIY+VKTD+KAQTLTMEG+IE EKFKSYLK KLQKH DIT    + DSSK      E
Sbjct: 125 KHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKHVDITVDTKSTDSSKSTAVASE 184

Query: 183 KKKES-SEQKEKPKEKSSSETTTTTTTK---IVAEIQTK-----DTDNNKNNAPYFVHYV 242
           KKKES +++KEKPKEK+SSETT TTT K   +VAEIQ+K     D +N  NN PYF+HYV
Sbjct: 185 KKKESNTDKKEKPKEKASSETTITTTDKKTIVVAEIQSKENNSNDINNKNNNVPYFIHYV 244

Query: 243 YAPQLFSDENPNACQVM 244
           YAPQLFSDENPNAC+VM
Sbjct: 245 YAPQLFSDENPNACRVM 261

[Close]

BLAST of CmoCh14G020150.1 vs. NCBI nr
Match: gi|659075593|ref|XP_008438227.1| (PREDICTED: uncharacterized protein DDB_G0286299 [Cucumis melo])

HSP 1 Score: 348.6 bits (893), Expect = 8.9e-93
Identity = 186/259 (71.81%), Postives = 216/259 (83.40%), Query Frame = 1


Query: 1   MGEKAEA---ITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVL 60
           MGEK +    ITAIYK+NLHC QCWR+IK+PL+ TQG+ NVEVDMEKNEIRVKGSNLDVL
Sbjct: 1   MGEKEKVEGIITAIYKLNLHCHQCWRDIKKPLSITQGVQNVEVDMEKNEIRVKGSNLDVL 60

Query: 61  KIHKQIEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDL 120
           KI KQIEKLSKK+V+LISPKVKPK+K+ PKP  +KPKP+++ RTITAKVHLHC KCE+DL
Sbjct: 61  KIQKQIEKLSKKKVELISPKVKPKDKDPPKPTADKPKPTIVNRTITAKVHLHCPKCEQDL 120

Query: 121 KNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSK----- 180
           KN LLKHKGIY+VKTD+KAQTLTMEG+IE EKFKSYLK KLQKH DIT    S+      
Sbjct: 121 KNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKHVDITVDTKSTDYSSKS 180

Query: 181 ---PPEKKKES-SEQKEKPKEKSSSETTTTTTTK----IVAEIQTKDTDNNKNNAPYFVH 240
                EKKKES ++QKEKPKEK+SS+TTTTTTT     +VAEIQ+K+ +N  +N PYF+H
Sbjct: 181 TAVASEKKKESNTDQKEKPKEKASSDTTTTTTTDKTTIVVAEIQSKENNNKNDNVPYFIH 240

Query: 241 YVYAPQLFSDENPNACQVM 244
           YVYAPQLFSDENPNAC+VM
Sbjct: 241 YVYAPQLFSDENPNACRVM 259

[Close]

BLAST of CmoCh14G020150.1 vs. NCBI nr
Match: gi|700201510|gb|KGN56643.1| (hypothetical protein Csa_3G127060 [Cucumis sativus])

HSP 1 Score: 284.3 bits (726), Expect = 2.1e-73
Identity = 161/218 (73.85%), Postives = 182/218 (83.49%), Query Frame = 1


Query: 42  MEKNEIRVKGSNLDVLKIHKQIEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRT 101
           MEKNEIRVKGSNLDVLKI KQIEKLSKK+V+LISPKVKPKEK+ PKP D+KPKP+++ R 
Sbjct: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60

Query: 102 ITAKVHLHCAKCEKDLKNNLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKH 161
           ITAKVHLHC KCE+DLKN LLKHKGIY+VKTD+KAQTLTMEG+IE EKFKSYLK KLQKH
Sbjct: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120

Query: 162 ADITT---AVDSSK----PPEKKKES-SEQKEKPKEKSSSETTTTTTTK---IVAEIQTK 221
            DIT    + DSSK      EKKKES +++KEKPKEK+SSETT TTT K   +VAEIQ+K
Sbjct: 121 VDITVDTKSTDSSKSTAVASEKKKESNTDKKEKPKEKASSETTITTTDKKTIVVAEIQSK 180

Query: 222 -----DTDNNKNNAPYFVHYVYAPQLFSDENPNACQVM 244
                D +N  NN PYF+HYVYAPQLFSDENPNAC+VM
Sbjct: 181 ENNSNDINNKNNNVPYFIHYVYAPQLFSDENPNACRVM 218

[Close]

BLAST of CmoCh14G020150.1 vs. NCBI nr
Match: gi|1009110398|ref|XP_015895358.1| (PREDICTED: transcription initiation factor TFIID subunit 3 [Ziziphus jujuba])

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-59
Identity = 144/267 (53.93%), Postives = 182/267 (68.16%), Query Frame = 1


Query: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62
           +K   I A+YKVN+HC++C  +IK+PL RTQG+ NVEVDMEK EIR KG   D +KI KQ
Sbjct: 46  KKDGIINAVYKVNMHCRKCAHDIKKPLMRTQGVQNVEVDMEKGEIRAKGI-FDPIKIQKQ 105

Query: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSL--IQRTITAKVHLHCAKCEKDLKNN 122
           IE+LSKK+ +LISPK++ KEK+T K  D KPK +   I RT + KVH+HC +CEKDLK  
Sbjct: 106 IERLSKKKTELISPKIQIKEKDTSKV-DQKPKETKQEISRTTSVKVHMHCEQCEKDLKKK 165

Query: 123 LLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTA------------- 182
           LLK K IY+VKTDMKAQTLT++GTIEPEK  + L+KK+ KHA+I  +             
Sbjct: 166 LLKRKDIYSVKTDMKAQTLTVDGTIEPEKLINLLRKKVHKHAEIIASKPDKKDEKKDMDK 225

Query: 183 --VDSSKPPEKKKESSEQKEKPKEKSSSETTTTTTTKI---------VAEIQTKDTDNNK 242
              D  K  EK ++  E+K+K KE  SS T+T+ + KI         V E++ KD     
Sbjct: 226 GEKDEKKDKEKGEKDEEKKDKEKEGKSS-TSTSESAKIVEINKEKTKVVEVKAKD----- 285

Query: 243 NNAPYFVHYVYAPQLFSDENPNACQVM 244
           +NAPYFVHYVYAPQLFSDENPNAC V+
Sbjct: 286 SNAPYFVHYVYAPQLFSDENPNACCVV 304

[Close]

BLAST of CmoCh14G020150.1 vs. NCBI nr
Match: gi|764630818|ref|XP_011469577.1| (PREDICTED: uncharacterized protein LOC101294110 isoform X2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 236.1 bits (601), Expect = 6.4e-59
Identity = 135/251 (53.78%), Postives = 174/251 (69.32%), Query Frame = 1


Query: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62
           EK   ITA+YKVNLHC QC REIK+PL   QG+HNVEV+ EK EI+V+G + D  KI K+
Sbjct: 25  EKDGVITAVYKVNLHCPQCAREIKKPLISIQGVHNVEVEREKGEIKVRG-DFDPTKIQKR 84

Query: 63  IEKLSKKRVDLISPKVKPKEKETPKP---NDNKPKPSLIQRTITAKVHLHCAKCEKDLKN 122
           +EKL KK+V+L+SPKV+ KE  T +     ++K K + I RT   KVH+HC KCE+DLK 
Sbjct: 85  LEKLCKKKVELVSPKVQIKESTTTETIVVKESKTKEA-ISRTTLVKVHMHCHKCEQDLKK 144

Query: 123 NLLKHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITTAVDSSKPPEKKKE 182
            LL +KG++NVKTDMKAQTL +EGTI+PEK  SYL+KK+ KHA+I       K   K+KE
Sbjct: 145 KLLTNKGVHNVKTDMKAQTLAVEGTIDPEKLVSYLRKKVHKHAEIIPPKQEKKDGTKEKE 204

Query: 183 SS-----EQKEKPKEKSSSETTTTTTTKIVA--EIQTKDTDNNKNNAPYFVHYVYAPQLF 242
            S     E+K++ KEK S ++T   T  + A  EI+  +    +  APYF+HYVYAPQ F
Sbjct: 205 KSSSKPPEKKDEKKEKESGKSTEIVTKVVEAKEEIKVVEVKTKEGGAPYFIHYVYAPQTF 264

Query: 243 SDENPNACQVM 244
           SDENPNAC +M
Sbjct: 265 SDENPNACSIM 273

The following BLAST results are available for this feature:

BLAST of CmoCh14G020150.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HIP3_ARATH	2.7e-17	28.36	Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana GN=...	[more]

BLAST of CmoCh14G020150.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4G4_CUCSA	1.4e-73	73.85	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G127060 PE=4 SV=1	[more]
B9SKJ8_RICCO	3.2e-57	53.31	Metal ion binding protein, putative OS=Ricinus communis GN=RCOM_0659680 PE=4 SV=...	[more]
A0A061FTM9_THECC	4.2e-57	54.66	Metal ion binding protein, putative OS=Theobroma cacao GN=TCM_011949 PE=4 SV=1	[more]
V4MQL6_EUTSA	1.8e-55	49.82	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10009467mg PE=4 SV=1	[more]
A0A059CE78_EUCGR	2.3e-55	47.83	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01129 PE=4 SV=1	[more]

BLAST of CmoCh14G020150.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G29000.1	9.2e-56	46.38	Heavy metal transport/detoxification superfamily protein	[more]
AT5G50740.3	3.0e-30	32.33	Heavy metal transport/detoxification superfamily protein	[more]
AT5G63530.1	4.9e-25	28.72	farnesylated protein 3	[more]
AT3G02960.1	3.6e-20	26.23	Heavy metal transport/detoxification superfamily protein	[more]
AT5G60800.2	4.5e-18	29.10	Heavy metal transport/detoxification superfamily protein	[more]

BLAST of CmoCh14G020150.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449433137\|ref\|XP_004134354.1\|	1.8e-93	73.93	PREDICTED: uncharacterized protein PF11_0207 [Cucumis sativus]	[more]
gi\|659075593\|ref\|XP_008438227.1\|	8.9e-93	71.81	PREDICTED: uncharacterized protein DDB_G0286299 [Cucumis melo]	[more]
gi\|700201510\|gb\|KGN56643.1\|	2.1e-73	73.85	hypothetical protein Csa_3G127060 [Cucumis sativus]	[more]
gi\|1009110398\|ref\|XP_015895358.1\|	1.3e-59	53.93	PREDICTED: transcription initiation factor TFIID subunit 3 [Ziziphus jujuba]	[more]
gi\|764630818\|ref\|XP_011469577.1\|	6.4e-59	53.78	PREDICTED: uncharacterized protein LOC101294110 isoform X2 [Fragaria vesca subsp...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030001	metal ion transport
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh14G020150	CmoCh14G020150	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh14G020150.1	CmoCh14G020150.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh14G020150.1.exon.1	CmoCh14G020150.1.exon.1	exon
CmoCh14G020150.1.exon.2	CmoCh14G020150.1.exon.2	exon
CmoCh14G020150.1.exon.3	CmoCh14G020150.1.exon.3	exon
CmoCh14G020150.1.exon.4	CmoCh14G020150.1.exon.4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh14G020150.1.CDS.1	CmoCh14G020150.1.CDS.1	CDS
CmoCh14G020150.1.CDS.2	CmoCh14G020150.1.CDS.2	CDS
CmoCh14G020150.1.CDS.3	CmoCh14G020150.1.CDS.3	CDS
CmoCh14G020150.1.CDS.4	CmoCh14G020150.1.CDS.4	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 107..160 score: 9.5E-13coord: 13..68 score: 2.0
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 8..72 score: 8
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 98..156 score: 5.89E-9coord: 10..69 score: 5.5
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 9..69 score: 2.7E-12coord: 99..163 score: 2.4
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 209..242 score: 8.2E-84coord: 4..163 score: 8.2
None	No IPR available	PANTHER	PTHR22814:SF148	F1K23.4	coord: 209..242 score: 8.2E-84coord: 4..163 score: 8.2