CmoCh13G003540.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh13G003540.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Subtilisin-like serine protease
Location	Cmo_Chr13 : 4540870 .. 4541551 (+)
Sequence length	588

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAGTCGTACTCGCCGGTGCCGTCAAAATGATTCTCTGCAATGATAGGCTTAGTGGATTTGAAATCATCGCCGATTTCCATGTTCTTCTAGCTTCCCGTATCAATTACAATGAAGGCCAAGTTGTTTCCTCGTATATCATGTTCTTCCAGCTTTCCATGTTCTAATACTTTGCAGCCGGACGTGACAGCCTCGGGAGTGAACATAATCATGGCATTCTTCGACGTCGTGAGCCCAACAAGGGAACCATTCGACAACAGAATAGTTTCATACATAACAATGTCAGGGACCTCCATGTCCTGTCGCCATGTCTCCGGCATTGTCGGCCTCCTCAAAGCTCTCCACCCCGAATGGAGCCCCGCCACCATCAAATCTGCCATAATGACCTCTACCAGAATTAGCGACAACACAATGAACCTCATGCTTGACGACGGCTCCCCTATCTTCGCTCCAGCCACCCCCTTCATATATGGTTCAGGGCACATCCGCCCCACTAGCGCCATCCACCCTGGTCTTGTCTACGACCTTTCCCCCAACAATTACTTGGAATTCCTCTGTGCCAGCGGCTACAAGGAGAAGAACATTCGAGTATTCGCTGATGGTTCTATTTTGAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGATTGGGAGTGTCACCAAGCGTTTTGAAGTTTGA

mRNA sequence

ATGCAAGTCGTACTCGCCGGTGCCGTCAAAATGATTCTCTGCAATGATAGGCTTAGTGGATTTGAAATCATCGCCGATTTCCATCCGGACGTGACAGCCTCGGGAGTGAACATAATCATGGCATTCTTCGACGTCGTGAGCCCAACAAGGGAACCATTCGACAACAGAATAGTTTCATACATAACAATGTCAGGGACCTCCATGTCCTGTCGCCATGTCTCCGGCATTGTCGGCCTCCTCAAAGCTCTCCACCCCGAATGGAGCCCCGCCACCATCAAATCTGCCATAATGACCTCTACCAGAATTAGCGACAACACAATGAACCTCATGCTTGACGACGGCTCCCCTATCTTCGCTCCAGCCACCCCCTTCATATATGGTTCAGGGCACATCCGCCCCACTAGCGCCATCCACCCTGGTCTTGTCTACGACCTTTCCCCCAACAATTACTTGGAATTCCTCTGTGCCAGCGGCTACAAGGAGAAGAACATTCGAGTATTCGCTGATGGTTCTATTTTGAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGATTGGGAGTGTCACCAAGCGTTTTGAAGTTTGA

Coding sequence (CDS)

ATGCAAGTCGTACTCGCCGGTGCCGTCAAAATGATTCTCTGCAATGATAGGCTTAGTGGATTTGAAATCATCGCCGATTTCCATCCGGACGTGACAGCCTCGGGAGTGAACATAATCATGGCATTCTTCGACGTCGTGAGCCCAACAAGGGAACCATTCGACAACAGAATAGTTTCATACATAACAATGTCAGGGACCTCCATGTCCTGTCGCCATGTCTCCGGCATTGTCGGCCTCCTCAAAGCTCTCCACCCCGAATGGAGCCCCGCCACCATCAAATCTGCCATAATGACCTCTACCAGAATTAGCGACAACACAATGAACCTCATGCTTGACGACGGCTCCCCTATCTTCGCTCCAGCCACCCCCTTCATATATGGTTCAGGGCACATCCGCCCCACTAGCGCCATCCACCCTGGTCTTGTCTACGACCTTTCCCCCAACAATTACTTGGAATTCCTCTGTGCCAGCGGCTACAAGGAGAAGAACATTCGAGTATTCGCTGATGGTTCTATTTTGAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGATTGGGAGTGTCACCAAGCGTTTTGAAGTTTGA

Homology

[Close]

BLAST of CmoCh13G003540.1 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.1e-45
Identity = 91/171 (53.22%), Postives = 115/171 (67.25%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GVNII AF +   PT    DNR   + T SGTSMSC H+SG+VGLLK LHP WS
Sbjct: 529 PDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWS 588

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT++R  +N    M+D+    F  A PF YGSGH++P  A HPGLVYDL+  
Sbjct: 589 PAAIRSAIMTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTG 648

Query: 149 NYLEFLCASGYKEKNIRVFAD---------GSILNFNYPSIGVQNLIGSVT 191
           +YL+FLCA GY    +++FA+          ++L+FNYPSI V NL GS+T
Sbjct: 649 DYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSIT 696

[Close]

BLAST of CmoCh13G003540.1 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.1e-37
Identity = 81/169 (47.93%), Postives = 107/169 (63.31%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GV++I A+   VSPT E FD R + +  +SGTSMSC H+SGI GLLK  +P WS
Sbjct: 523 PDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWS 582

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT+  I D+    + +  +     ATPF +G+GH++P  A++PGLVYDL   
Sbjct: 583 PAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIK 642

Query: 149 NYLEFLCASGYKEKNIRVFADG---------SILNFNYPSIGVQNLIGS 189
           +YL FLC+ GY    I VF+           S++N NYPSI V NL  S
Sbjct: 643 DYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS 688

[Close]

BLAST of CmoCh13G003540.1 vs. Swiss-Prot
Match: SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 85/167 (50.90%), Postives = 100/167 (59.88%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRI-VSYITMSGTSMSCRHVSGIVGLLKALHPEW 88
           PDVTA GVNI+ A+ +  S +    DNR    +  + GTSMSC H SGI GLLK  HP W
Sbjct: 537 PDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSW 596

Query: 89  SPATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSP 148
           SPA IKSAIMT+    DNT   + D      A A  F YGSGH+RP  AI PGLVYDLS 
Sbjct: 597 SPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSL 656

Query: 149 NNYLEFLCASGYKEKNI------RVF---ADGSILNFNYPSIGVQNL 186
            +YL FLCASGY ++ I      R F      S+ + NYPSI + NL
Sbjct: 657 TDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNL 701

[Close]

BLAST of CmoCh13G003540.1 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 9.8e-34
Identity = 73/162 (45.06%), Postives = 102/162 (62.96%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+ D V PT  P D R   +  +SGTSM+C HVSG   LLK+ HP+WS
Sbjct: 500 PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 559

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SA+MT+T + DN+   ++D+ +     ATP+ YGSGH+    A++PGLVYD++ +
Sbjct: 560 PAVIRSAMMTTTNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITND 619

Query: 149 NYLEFLCASGYKEKNIRVFADGSIL----------NFNYPSI 181
           +Y+ FLC+ GY  K I+V     +           N NYPSI
Sbjct: 620 DYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSI 659

[Close]

BLAST of CmoCh13G003540.1 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-31
Identity = 74/169 (43.79%), Postives = 98/169 (57.99%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+     PT    D+R V +  +SGTSMSC HVSG+  LLK++HPEWS
Sbjct: 504 PDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWS 563

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIF-----APATPFIYGSGHIRPTSAIHPGLVY 148
           PA I+SA+MT       T      DG P+       P+TPF +G+GH+ PT+A +PGL+Y
Sbjct: 564 PAAIRSALMT-------TAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 623

Query: 149 DLSPNNYLEFLCASGYKEKNIRVFA----------DGSILNFNYPSIGV 183
           DL+  +YL FLCA  Y    IR  +            S+ + NYPS  V
Sbjct: 624 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665

[Close]

BLAST of CmoCh13G003540.1 vs. TrEMBL
Match: A0A0A0LVY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171040 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-64
Identity = 126/170 (74.12%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP+WS
Sbjct: 506 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 565

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  AP+TPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 566 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 625

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILN NYPSIGVQNL GSVT
Sbjct: 626 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVT 675

[Close]

BLAST of CmoCh13G003540.1 vs. TrEMBL
Match: A0A0A0LYF1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171030 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 3.0e-58
Identity = 113/170 (66.47%), Postives = 132/170 (77.65%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GV+II AF + +SPTR+P DNR   +ITMSGTSMSC HV+G+VGLL+ LHP+W+
Sbjct: 524 PDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWT 583

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS ++ DNT+N MLD GS    PATPF YGSGHI PT A+ PGLVYDLSPN
Sbjct: 584 PSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPN 643

Query: 149 NYLEFLCASGYKEKNIRVFAD--------GSILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY E+ IR F+D         S+LN NYPSIGVQNL  SVT
Sbjct: 644 DYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVT 693

[Close]

BLAST of CmoCh13G003540.1 vs. TrEMBL
Match: A0A0J8BGA7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066150 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 7.0e-47
Identity = 99/174 (56.90%), Postives = 121/174 (69.54%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GV++I A+   VSPT EP D+R VSY+  SGTSMSC H+SGIVGLLK LHP WS
Sbjct: 514 PDITAPGVDVIAAYSQAVSPTDEPSDHRRVSYMMDSGTSMSCPHISGIVGLLKTLHPTWS 573

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT+ R  DNT+N M D     +A ATPF YG+GH+RPT A+ PGL+YDL+  
Sbjct: 574 PAAIRSAIMTTARTRDNTINPMEDAN---YAKATPFGYGAGHVRPTRAMDPGLIYDLNAT 633

Query: 149 NYLEFLCASGYKEKNIRVFADGS------------ILNFNYPSIGVQNLIGSVT 191
           +YL+FLC+ GY +  I  F +GS            +LNFNYPSI V  L GSVT
Sbjct: 634 DYLQFLCSIGYNKTTIETFYNGSHKCSKSSYNKENLLNFNYPSITVPKLSGSVT 684

[Close]

BLAST of CmoCh13G003540.1 vs. TrEMBL
Match: K7MJ92_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_17G004100 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-46
Identity = 99/170 (58.24%), Postives = 120/170 (70.59%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII A+ + VSPT   FD R V +ITMSGTSMSC HV+G+VGLLK LHP+WS
Sbjct: 130 PDVTAPGVNIIAAYSEGVSPTDMNFDKRRVPFITMSGTSMSCPHVAGVVGLLKTLHPDWS 189

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P  IKSA++T+ R  DNT   MLD G+   A ATPF YGSGHIRP  A+ PGLVYDL+ N
Sbjct: 190 PTVIKSALLTTARTRDNTGKPMLDGGNN--ANATPFAYGSGHIRPNRAMDPGLVYDLTNN 249

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YL FLC SGY +  I +F+          +IL+FNYP+I +  L GSV+
Sbjct: 250 DYLNFLCVSGYNQSQIEMFSGAHYRCPDIINILDFNYPTITIPKLYGSVS 297

[Close]

BLAST of CmoCh13G003540.1 vs. TrEMBL
Match: A0A068B924_CICAR (Subtilase OS=Cicer arietinum PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-46
Identity = 101/169 (59.76%), Postives = 120/169 (71.01%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII A+   VSPT   FD R V +ITMSGTSMSC HV+GIVGLLK LHP+WS
Sbjct: 510 PDVTAPGVNIIAAYSGAVSPTEMEFDKRRVPFITMSGTSMSCPHVAGIVGLLKTLHPDWS 569

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMT+ R  DNT   +LD+ +     ATPF YGSGHIRP  A+ PGLVY+L+ N
Sbjct: 570 PSAIKSAIMTTARTRDNTAKPILDNNN---MKATPFAYGSGHIRPNRAMDPGLVYELNIN 629

Query: 149 NYLEFLCASGYKEKNIRVFA-------DGSILNFNYPSIGVQNLIGSVT 191
           +YL FLC  GY +  I++F+       D +IL+FNYPSI V  L GSVT
Sbjct: 630 DYLNFLCFLGYNKTQIKMFSGNHYHCHDINILDFNYPSITVPKLYGSVT 675

[Close]

BLAST of CmoCh13G003540.1 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 184.5 bits (467), Expect = 6.3e-47
Identity = 91/171 (53.22%), Postives = 115/171 (67.25%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GVNII AF +   PT    DNR   + T SGTSMSC H+SG+VGLLK LHP WS
Sbjct: 529 PDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWS 588

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT++R  +N    M+D+    F  A PF YGSGH++P  A HPGLVYDL+  
Sbjct: 589 PAAIRSAIMTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTG 648

Query: 149 NYLEFLCASGYKEKNIRVFAD---------GSILNFNYPSIGVQNLIGSVT 191
           +YL+FLCA GY    +++FA+          ++L+FNYPSI V NL GS+T
Sbjct: 649 DYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSIT 696

[Close]

BLAST of CmoCh13G003540.1 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 157.9 bits (398), Expect = 6.3e-39
Identity = 81/169 (47.93%), Postives = 107/169 (63.31%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GV++I A+   VSPT E FD R + +  +SGTSMSC H+SGI GLLK  +P WS
Sbjct: 523 PDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWS 582

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT+  I D+    + +  +     ATPF +G+GH++P  A++PGLVYDL   
Sbjct: 583 PAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIK 642

Query: 149 NYLEFLCASGYKEKNIRVFADG---------SILNFNYPSIGVQNLIGS 189
           +YL FLC+ GY    I VF+           S++N NYPSI V NL  S
Sbjct: 643 DYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS 688

[Close]

BLAST of CmoCh13G003540.1 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 144.8 bits (364), Expect = 5.5e-35
Identity = 73/162 (45.06%), Postives = 102/162 (62.96%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+ D V PT  P D R   +  +SGTSM+C HVSG   LLK+ HP+WS
Sbjct: 500 PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 559

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SA+MT+T + DN+   ++D+ +     ATP+ YGSGH+    A++PGLVYD++ +
Sbjct: 560 PAVIRSAMMTTTNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITND 619

Query: 149 NYLEFLCASGYKEKNIRVFADGSIL----------NFNYPSI 181
           +Y+ FLC+ GY  K I+V     +           N NYPSI
Sbjct: 620 DYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSI 659

[Close]

BLAST of CmoCh13G003540.1 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 137.9 bits (346), Expect = 6.7e-33
Identity = 74/169 (43.79%), Postives = 98/169 (57.99%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+     PT    D+R V +  +SGTSMSC HVSG+  LLK++HPEWS
Sbjct: 504 PDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWS 563

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIF-----APATPFIYGSGHIRPTSAIHPGLVY 148
           PA I+SA+MT       T      DG P+       P+TPF +G+GH+ PT+A +PGL+Y
Sbjct: 564 PAAIRSALMT-------TAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 623

Query: 149 DLSPNNYLEFLCASGYKEKNIRVFA----------DGSILNFNYPSIGV 183
           DL+  +YL FLCA  Y    IR  +            S+ + NYPS  V
Sbjct: 624 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665

[Close]

BLAST of CmoCh13G003540.1 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-32
Identity = 72/164 (43.90%), Postives = 95/164 (57.93%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDV   GVNI+  + D + PT    D+R   +  MSGTSMSC H+SG+ GLLKA HPEWS
Sbjct: 498 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 557

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSA+MT+  + DNT N  L D +   + + P+ +GSGH+ P  A+ PGLVYD+S  
Sbjct: 558 PSAIKSALMTTAYVLDNT-NAPLHDAAD-NSLSNPYAHGSGHVDPQKALSPGLVYDISTE 617

Query: 149 NYLEFLCASGYKEKNIRVFADGSILN----------FNYPSIGV 183
            Y+ FLC+  Y   +I        +N           NYPS  V
Sbjct: 618 EYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 659

[Close]

BLAST of CmoCh13G003540.1 vs. NCBI nr
Match: gi|659128687|ref|XP_008464322.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 256.5 bits (654), Expect = 3.7e-65
Identity = 128/170 (75.29%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP WS
Sbjct: 525 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWS 584

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  APATPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 585 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPN 644

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILNFNYPSIGVQNL GSVT
Sbjct: 645 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT 694

[Close]

BLAST of CmoCh13G003540.1 vs. NCBI nr
Match: gi|449443664|ref|XP_004139597.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-64
Identity = 126/170 (74.12%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP+WS
Sbjct: 500 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 559

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  AP+TPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 560 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 619

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILN NYPSIGVQNL GSVT
Sbjct: 620 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVT 669

[Close]

BLAST of CmoCh13G003540.1 vs. NCBI nr
Match: gi|700209886|gb|KGN64982.1| (hypothetical protein Csa_1G171040 [Cucumis sativus])

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-64
Identity = 126/170 (74.12%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP+WS
Sbjct: 506 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 565

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  AP+TPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 566 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 625

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILN NYPSIGVQNL GSVT
Sbjct: 626 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVT 675

[Close]

BLAST of CmoCh13G003540.1 vs. NCBI nr
Match: gi|659128619|ref|XP_008464289.1| (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-59
Identity = 116/170 (68.24%), Postives = 134/170 (78.82%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + +SPTR+  DNR   +ITMSGTSMSC HV+G+VGLL+ LHP+WS
Sbjct: 523 PDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWS 582

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS+++ DNT+N M+D GS   APATPF YGSGHI PT AI PGLVYDLSPN
Sbjct: 583 PSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPN 642

Query: 149 NYLEFLCASGYKEKNIRVFAD--------GSILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK IR F+D         S+LN NYPSIGVQNL GSV+
Sbjct: 643 DYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQNLKGSVS 692

[Close]

BLAST of CmoCh13G003540.1 vs. NCBI nr
Match: gi|778665004|ref|XP_011648463.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 4.4e-58
Identity = 113/170 (66.47%), Postives = 132/170 (77.65%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GV+II AF + +SPTR+P DNR   +ITMSGTSMSC HV+G+VGLL+ LHP+W+
Sbjct: 524 PDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWT 583

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS ++ DNT+N MLD GS    PATPF YGSGHI PT A+ PGLVYDLSPN
Sbjct: 584 PSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPN 643

Query: 149 NYLEFLCASGYKEKNIRVFAD--------GSILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY E+ IR F+D         S+LN NYPSIGVQNL  SVT
Sbjct: 644 DYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVT 693

The following BLAST results are available for this feature:

BLAST of CmoCh13G003540.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT54_ARATH	1.1e-45	53.22	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1	[more]
AIR3_ARATH	1.1e-37	47.93	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1	[more]
SBT1_SOYBN	2.6e-34	50.90	Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3	[more]
SBT16_ARATH	9.8e-34	45.06	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1	[more]
SBT17_ARATH	1.2e-31	43.79	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1	[more]

BLAST of CmoCh13G003540.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVY8_CUCSA	1.7e-64	74.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171040 PE=4 SV=1	[more]
A0A0A0LYF1_CUCSA	3.0e-58	66.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171030 PE=4 SV=1	[more]
A0A0J8BGA7_BETVU	7.0e-47	56.90	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066150 PE=4 S...	[more]
K7MJ92_SOYBN	1.2e-46	58.24	Uncharacterized protein OS=Glycine max GN=GLYMA_17G004100 PE=4 SV=1	[more]
A0A068B924_CICAR	1.2e-46	59.76	Subtilase OS=Cicer arietinum PE=4 SV=1	[more]

BLAST of CmoCh13G003540.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G59810.1	6.3e-47	53.22	Subtilase family protein	[more]
AT2G04160.1	6.3e-39	47.93	Subtilisin-like serine endopeptidase family protein	[more]
AT4G34980.1	5.5e-35	45.06	subtilisin-like serine protease 2	[more]
AT5G67360.1	6.7e-33	43.79	Subtilase family protein	[more]
AT2G05920.1	1.1e-32	43.90	Subtilase family protein	[more]

BLAST of CmoCh13G003540.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659128687\|ref\|XP_008464322.1\|	3.7e-65	75.29	PREDICTED: subtilisin-like protease [Cucumis melo]	[more]
gi\|449443664\|ref\|XP_004139597.1\|	2.4e-64	74.12	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
gi\|700209886\|gb\|KGN64982.1\|	2.4e-64	74.12	hypothetical protein Csa_1G171040 [Cucumis sativus]	[more]
gi\|659128619\|ref\|XP_008464289.1\|	1.4e-59	68.24	PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo]	[more]
gi\|778665004\|ref\|XP_011648463.1\|	4.4e-58	66.47	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000209	Peptidase_S8/S53_dom
IPR015500	Peptidase_S8_subtilisin-rel

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh13G003540	CmoCh13G003540	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh13G003540.1	CmoCh13G003540.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh13G003540.1.exon.1	CmoCh13G003540.1.exon.1	exon
CmoCh13G003540.1.exon.2	CmoCh13G003540.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh13G003540.1.CDS.1	CmoCh13G003540.1.CDS.1	CDS
CmoCh13G003540.1.CDS.2	CmoCh13G003540.1.CDS.2	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	GENE3D	G3DSA:3.40.50.200		coord: 24..151 score: 7.4
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 26..103 score: 1.9
IPR000209	Peptidase S8/S53 domain	unknown	SSF52743	Subtilisin-like	coord: 13..137 score: 6.81
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..190 score: 1.4
None	No IPR available	PANTHER	PTHR10795:SF3	SUBFAMILY NOT NAMED	coord: 1..190 score: 1.4