CmoCh13G003540 (gene) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh13G003540
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Subtilisin-like serine protease
Location	Cmo_Chr13 : 4540870 .. 4541551 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAGTCGTACTCGCCGGTGCCGTCAAAATGATTCTCTGCAATGATAGGCTTAGTGGATTTGAAATCATCGCCGATTTCCATGTTCTTCTAGCTTCCCGTATCAATTACAATGAAGGCCAAGTTGTTTCCTCGTATATCATGTTCTTCCAGCTTTCCATGTTCTAATACTTTGCAGCCGGACGTGACAGCCTCGGGAGTGAACATAATCATGGCATTCTTCGACGTCGTGAGCCCAACAAGGGAACCATTCGACAACAGAATAGTTTCATACATAACAATGTCAGGGACCTCCATGTCCTGTCGCCATGTCTCCGGCATTGTCGGCCTCCTCAAAGCTCTCCACCCCGAATGGAGCCCCGCCACCATCAAATCTGCCATAATGACCTCTACCAGAATTAGCGACAACACAATGAACCTCATGCTTGACGACGGCTCCCCTATCTTCGCTCCAGCCACCCCCTTCATATATGGTTCAGGGCACATCCGCCCCACTAGCGCCATCCACCCTGGTCTTGTCTACGACCTTTCCCCCAACAATTACTTGGAATTCCTCTGTGCCAGCGGCTACAAGGAGAAGAACATTCGAGTATTCGCTGATGGTTCTATTTTGAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGATTGGGAGTGTCACCAAGCGTTTTGAAGTTTGA

mRNA sequence

ATGCAAGTCGTACTCGCCGGTGCCGTCAAAATGATTCTCTGCAATGATAGGCTTAGTGGATTTGAAATCATCGCCGATTTCCATCCGGACGTGACAGCCTCGGGAGTGAACATAATCATGGCATTCTTCGACGTCGTGAGCCCAACAAGGGAACCATTCGACAACAGAATAGTTTCATACATAACAATGTCAGGGACCTCCATGTCCTGTCGCCATGTCTCCGGCATTGTCGGCCTCCTCAAAGCTCTCCACCCCGAATGGAGCCCCGCCACCATCAAATCTGCCATAATGACCTCTACCAGAATTAGCGACAACACAATGAACCTCATGCTTGACGACGGCTCCCCTATCTTCGCTCCAGCCACCCCCTTCATATATGGTTCAGGGCACATCCGCCCCACTAGCGCCATCCACCCTGGTCTTGTCTACGACCTTTCCCCCAACAATTACTTGGAATTCCTCTGTGCCAGCGGCTACAAGGAGAAGAACATTCGAGTATTCGCTGATGGTTCTATTTTGAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGATTGGGAGTGTCACCAAGCGTTTTGAAGTTTGA

Coding sequence (CDS)

ATGCAAGTCGTACTCGCCGGTGCCGTCAAAATGATTCTCTGCAATGATAGGCTTAGTGGATTTGAAATCATCGCCGATTTCCATCCGGACGTGACAGCCTCGGGAGTGAACATAATCATGGCATTCTTCGACGTCGTGAGCCCAACAAGGGAACCATTCGACAACAGAATAGTTTCATACATAACAATGTCAGGGACCTCCATGTCCTGTCGCCATGTCTCCGGCATTGTCGGCCTCCTCAAAGCTCTCCACCCCGAATGGAGCCCCGCCACCATCAAATCTGCCATAATGACCTCTACCAGAATTAGCGACAACACAATGAACCTCATGCTTGACGACGGCTCCCCTATCTTCGCTCCAGCCACCCCCTTCATATATGGTTCAGGGCACATCCGCCCCACTAGCGCCATCCACCCTGGTCTTGTCTACGACCTTTCCCCCAACAATTACTTGGAATTCCTCTGTGCCAGCGGCTACAAGGAGAAGAACATTCGAGTATTCGCTGATGGTTCTATTTTGAACTTTAATTACCCTTCCATTGGGGTTCAGAACTTGATTGGGAGTGTCACCAAGCGTTTTGAAGTTTGA

Homology

[Close]

BLAST of CmoCh13G003540 vs. Swiss-Prot
Match: SBT54_ARATH (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.1e-45
Identity = 91/171 (53.22%), Postives = 115/171 (67.25%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GVNII AF +   PT    DNR   + T SGTSMSC H+SG+VGLLK LHP WS
Sbjct: 529 PDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWS 588

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT++R  +N    M+D+    F  A PF YGSGH++P  A HPGLVYDL+  
Sbjct: 589 PAAIRSAIMTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTG 648

Query: 149 NYLEFLCASGYKEKNIRVFAD---------GSILNFNYPSIGVQNLIGSVT 191
           +YL+FLCA GY    +++FA+          ++L+FNYPSI V NL GS+T
Sbjct: 649 DYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSIT 696

[Close]

BLAST of CmoCh13G003540 vs. Swiss-Prot
Match: AIR3_ARATH (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.1e-37
Identity = 81/169 (47.93%), Postives = 107/169 (63.31%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GV++I A+   VSPT E FD R + +  +SGTSMSC H+SGI GLLK  +P WS
Sbjct: 523 PDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWS 582

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT+  I D+    + +  +     ATPF +G+GH++P  A++PGLVYDL   
Sbjct: 583 PAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIK 642

Query: 149 NYLEFLCASGYKEKNIRVFADG---------SILNFNYPSIGVQNLIGS 189
           +YL FLC+ GY    I VF+           S++N NYPSI V NL  S
Sbjct: 643 DYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS 688

[Close]

BLAST of CmoCh13G003540 vs. Swiss-Prot
Match: SBT1_SOYBN (Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 85/167 (50.90%), Postives = 100/167 (59.88%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRI-VSYITMSGTSMSCRHVSGIVGLLKALHPEW 88
           PDVTA GVNI+ A+ +  S +    DNR    +  + GTSMSC H SGI GLLK  HP W
Sbjct: 537 PDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSW 596

Query: 89  SPATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSP 148
           SPA IKSAIMT+    DNT   + D      A A  F YGSGH+RP  AI PGLVYDLS 
Sbjct: 597 SPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSL 656

Query: 149 NNYLEFLCASGYKEKNI------RVF---ADGSILNFNYPSIGVQNL 186
            +YL FLCASGY ++ I      R F      S+ + NYPSI + NL
Sbjct: 657 TDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNL 701

[Close]

BLAST of CmoCh13G003540 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 9.8e-34
Identity = 73/162 (45.06%), Postives = 102/162 (62.96%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+ D V PT  P D R   +  +SGTSM+C HVSG   LLK+ HP+WS
Sbjct: 500 PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 559

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SA+MT+T + DN+   ++D+ +     ATP+ YGSGH+    A++PGLVYD++ +
Sbjct: 560 PAVIRSAMMTTTNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITND 619

Query: 149 NYLEFLCASGYKEKNIRVFADGSIL----------NFNYPSI 181
           +Y+ FLC+ GY  K I+V     +           N NYPSI
Sbjct: 620 DYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSI 659

[Close]

BLAST of CmoCh13G003540 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-31
Identity = 74/169 (43.79%), Postives = 98/169 (57.99%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+     PT    D+R V +  +SGTSMSC HVSG+  LLK++HPEWS
Sbjct: 504 PDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWS 563

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIF-----APATPFIYGSGHIRPTSAIHPGLVY 148
           PA I+SA+MT       T      DG P+       P+TPF +G+GH+ PT+A +PGL+Y
Sbjct: 564 PAAIRSALMT-------TAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 623

Query: 149 DLSPNNYLEFLCASGYKEKNIRVFA----------DGSILNFNYPSIGV 183
           DL+  +YL FLCA  Y    IR  +            S+ + NYPS  V
Sbjct: 624 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665

[Close]

BLAST of CmoCh13G003540 vs. TrEMBL
Match: A0A0A0LVY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171040 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-64
Identity = 126/170 (74.12%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP+WS
Sbjct: 506 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 565

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  AP+TPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 566 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 625

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILN NYPSIGVQNL GSVT
Sbjct: 626 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVT 675

[Close]

BLAST of CmoCh13G003540 vs. TrEMBL
Match: A0A0A0LYF1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171030 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 3.0e-58
Identity = 113/170 (66.47%), Postives = 132/170 (77.65%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GV+II AF + +SPTR+P DNR   +ITMSGTSMSC HV+G+VGLL+ LHP+W+
Sbjct: 524 PDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWT 583

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS ++ DNT+N MLD GS    PATPF YGSGHI PT A+ PGLVYDLSPN
Sbjct: 584 PSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPN 643

Query: 149 NYLEFLCASGYKEKNIRVFAD--------GSILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY E+ IR F+D         S+LN NYPSIGVQNL  SVT
Sbjct: 644 DYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVT 693

[Close]

BLAST of CmoCh13G003540 vs. TrEMBL
Match: A0A0J8BGA7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066150 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 7.0e-47
Identity = 99/174 (56.90%), Postives = 121/174 (69.54%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GV++I A+   VSPT EP D+R VSY+  SGTSMSC H+SGIVGLLK LHP WS
Sbjct: 514 PDITAPGVDVIAAYSQAVSPTDEPSDHRRVSYMMDSGTSMSCPHISGIVGLLKTLHPTWS 573

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT+ R  DNT+N M D     +A ATPF YG+GH+RPT A+ PGL+YDL+  
Sbjct: 574 PAAIRSAIMTTARTRDNTINPMEDAN---YAKATPFGYGAGHVRPTRAMDPGLIYDLNAT 633

Query: 149 NYLEFLCASGYKEKNIRVFADGS------------ILNFNYPSIGVQNLIGSVT 191
           +YL+FLC+ GY +  I  F +GS            +LNFNYPSI V  L GSVT
Sbjct: 634 DYLQFLCSIGYNKTTIETFYNGSHKCSKSSYNKENLLNFNYPSITVPKLSGSVT 684

[Close]

BLAST of CmoCh13G003540 vs. TrEMBL
Match: K7MJ92_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_17G004100 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-46
Identity = 99/170 (58.24%), Postives = 120/170 (70.59%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII A+ + VSPT   FD R V +ITMSGTSMSC HV+G+VGLLK LHP+WS
Sbjct: 130 PDVTAPGVNIIAAYSEGVSPTDMNFDKRRVPFITMSGTSMSCPHVAGVVGLLKTLHPDWS 189

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P  IKSA++T+ R  DNT   MLD G+   A ATPF YGSGHIRP  A+ PGLVYDL+ N
Sbjct: 190 PTVIKSALLTTARTRDNTGKPMLDGGNN--ANATPFAYGSGHIRPNRAMDPGLVYDLTNN 249

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YL FLC SGY +  I +F+          +IL+FNYP+I +  L GSV+
Sbjct: 250 DYLNFLCVSGYNQSQIEMFSGAHYRCPDIINILDFNYPTITIPKLYGSVS 297

[Close]

BLAST of CmoCh13G003540 vs. TrEMBL
Match: A0A068B924_CICAR (Subtilase OS=Cicer arietinum PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-46
Identity = 101/169 (59.76%), Postives = 120/169 (71.01%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII A+   VSPT   FD R V +ITMSGTSMSC HV+GIVGLLK LHP+WS
Sbjct: 510 PDVTAPGVNIIAAYSGAVSPTEMEFDKRRVPFITMSGTSMSCPHVAGIVGLLKTLHPDWS 569

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMT+ R  DNT   +LD+ +     ATPF YGSGHIRP  A+ PGLVY+L+ N
Sbjct: 570 PSAIKSAIMTTARTRDNTAKPILDNNN---MKATPFAYGSGHIRPNRAMDPGLVYELNIN 629

Query: 149 NYLEFLCASGYKEKNIRVFA-------DGSILNFNYPSIGVQNLIGSVT 191
           +YL FLC  GY +  I++F+       D +IL+FNYPSI V  L GSVT
Sbjct: 630 DYLNFLCFLGYNKTQIKMFSGNHYHCHDINILDFNYPSITVPKLYGSVT 675

[Close]

BLAST of CmoCh13G003540 vs. TAIR10
Match: AT5G59810.1 (AT5G59810.1 Subtilase family protein)

HSP 1 Score: 184.5 bits (467), Expect = 6.3e-47
Identity = 91/171 (53.22%), Postives = 115/171 (67.25%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GVNII AF +   PT    DNR   + T SGTSMSC H+SG+VGLLK LHP WS
Sbjct: 529 PDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWS 588

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT++R  +N    M+D+    F  A PF YGSGH++P  A HPGLVYDL+  
Sbjct: 589 PAAIRSAIMTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTG 648

Query: 149 NYLEFLCASGYKEKNIRVFAD---------GSILNFNYPSIGVQNLIGSVT 191
           +YL+FLCA GY    +++FA+          ++L+FNYPSI V NL GS+T
Sbjct: 649 DYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSIT 696

[Close]

BLAST of CmoCh13G003540 vs. TAIR10
Match: AT2G04160.1 (AT2G04160.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 157.9 bits (398), Expect = 6.3e-39
Identity = 81/169 (47.93%), Postives = 107/169 (63.31%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+TA GV++I A+   VSPT E FD R + +  +SGTSMSC H+SGI GLLK  +P WS
Sbjct: 523 PDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWS 582

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SAIMT+  I D+    + +  +     ATPF +G+GH++P  A++PGLVYDL   
Sbjct: 583 PAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIK 642

Query: 149 NYLEFLCASGYKEKNIRVFADG---------SILNFNYPSIGVQNLIGS 189
           +YL FLC+ GY    I VF+           S++N NYPSI V NL  S
Sbjct: 643 DYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS 688

[Close]

BLAST of CmoCh13G003540 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 144.8 bits (364), Expect = 5.5e-35
Identity = 73/162 (45.06%), Postives = 102/162 (62.96%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+ D V PT  P D R   +  +SGTSM+C HVSG   LLK+ HP+WS
Sbjct: 500 PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWS 559

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           PA I+SA+MT+T + DN+   ++D+ +     ATP+ YGSGH+    A++PGLVYD++ +
Sbjct: 560 PAVIRSAMMTTTNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITND 619

Query: 149 NYLEFLCASGYKEKNIRVFADGSIL----------NFNYPSI 181
           +Y+ FLC+ GY  K I+V     +           N NYPSI
Sbjct: 620 DYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSI 659

[Close]

BLAST of CmoCh13G003540 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 137.9 bits (346), Expect = 6.7e-33
Identity = 74/169 (43.79%), Postives = 98/169 (57.99%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PD+ A GVNI+ A+     PT    D+R V +  +SGTSMSC HVSG+  LLK++HPEWS
Sbjct: 504 PDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWS 563

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIF-----APATPFIYGSGHIRPTSAIHPGLVY 148
           PA I+SA+MT       T      DG P+       P+TPF +G+GH+ PT+A +PGL+Y
Sbjct: 564 PAAIRSALMT-------TAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 623

Query: 149 DLSPNNYLEFLCASGYKEKNIRVFA----------DGSILNFNYPSIGV 183
           DL+  +YL FLCA  Y    IR  +            S+ + NYPS  V
Sbjct: 624 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV 665

[Close]

BLAST of CmoCh13G003540 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-32
Identity = 72/164 (43.90%), Postives = 95/164 (57.93%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDV   GVNI+  + D + PT    D+R   +  MSGTSMSC H+SG+ GLLKA HPEWS
Sbjct: 498 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 557

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSA+MT+  + DNT N  L D +   + + P+ +GSGH+ P  A+ PGLVYD+S  
Sbjct: 558 PSAIKSALMTTAYVLDNT-NAPLHDAAD-NSLSNPYAHGSGHVDPQKALSPGLVYDISTE 617

Query: 149 NYLEFLCASGYKEKNIRVFADGSILN----------FNYPSIGV 183
            Y+ FLC+  Y   +I        +N           NYPS  V
Sbjct: 618 EYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 659

[Close]

BLAST of CmoCh13G003540 vs. NCBI nr
Match: gi|659128687|ref|XP_008464322.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 256.5 bits (654), Expect = 3.7e-65
Identity = 128/170 (75.29%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP WS
Sbjct: 525 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWS 584

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  APATPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 585 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPN 644

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILNFNYPSIGVQNL GSVT
Sbjct: 645 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVT 694

[Close]

BLAST of CmoCh13G003540 vs. NCBI nr
Match: gi|449443664|ref|XP_004139597.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-64
Identity = 126/170 (74.12%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP+WS
Sbjct: 500 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 559

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  AP+TPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 560 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 619

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILN NYPSIGVQNL GSVT
Sbjct: 620 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVT 669

[Close]

BLAST of CmoCh13G003540 vs. NCBI nr
Match: gi|700209886|gb|KGN64982.1| (hypothetical protein Csa_1G171040 [Cucumis sativus])

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-64
Identity = 126/170 (74.12%), Postives = 136/170 (80.00%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + VSPT EPFDNR V +ITMSGTSMSC HVSG+VGLL+ LHP+WS
Sbjct: 506 PDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 565

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS RI DNT   MLD GSP  AP+TPF YGSGHIRPT AI PGLVYDLSPN
Sbjct: 566 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 625

Query: 149 NYLEFLCASGYKEKNIRVFADG--------SILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK I+ F+DG        SILN NYPSIGVQNL GSVT
Sbjct: 626 DYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVT 675

[Close]

BLAST of CmoCh13G003540 vs. NCBI nr
Match: gi|659128619|ref|XP_008464289.1| (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-59
Identity = 116/170 (68.24%), Postives = 134/170 (78.82%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GVNII AF + +SPTR+  DNR   +ITMSGTSMSC HV+G+VGLL+ LHP+WS
Sbjct: 523 PDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWS 582

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS+++ DNT+N M+D GS   APATPF YGSGHI PT AI PGLVYDLSPN
Sbjct: 583 PSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPN 642

Query: 149 NYLEFLCASGYKEKNIRVFAD--------GSILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY EK IR F+D         S+LN NYPSIGVQNL GSV+
Sbjct: 643 DYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQNLKGSVS 692

[Close]

BLAST of CmoCh13G003540 vs. NCBI nr
Match: gi|778665004|ref|XP_011648463.1| (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 4.4e-58
Identity = 113/170 (66.47%), Postives = 132/170 (77.65%), Query Frame = 1


Query: 29  PDVTASGVNIIMAFFDVVSPTREPFDNRIVSYITMSGTSMSCRHVSGIVGLLKALHPEWS 88
           PDVTA GV+II AF + +SPTR+P DNR   +ITMSGTSMSC HV+G+VGLL+ LHP+W+
Sbjct: 524 PDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWT 583

Query: 89  PATIKSAIMTSTRISDNTMNLMLDDGSPIFAPATPFIYGSGHIRPTSAIHPGLVYDLSPN 148
           P+ IKSAIMTS ++ DNT+N MLD GS    PATPF YGSGHI PT A+ PGLVYDLSPN
Sbjct: 584 PSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPN 643

Query: 149 NYLEFLCASGYKEKNIRVFAD--------GSILNFNYPSIGVQNLIGSVT 191
           +YLEFLCASGY E+ IR F+D         S+LN NYPSIGVQNL  SVT
Sbjct: 644 DYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNYPSIGVQNLKDSVT 693

The following BLAST results are available for this feature:

BLAST of CmoCh13G003540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBT54_ARATH	1.1e-45	53.22	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana GN=SBT5.4 PE=1 SV=1	[more]
AIR3_ARATH	1.1e-37	47.93	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana GN=AIR3 PE=2 SV=1	[more]
SBT1_SOYBN	2.6e-34	50.90	Subtilisin-like protease Glyma18g48580 OS=Glycine max GN=Glyma18g48580 PE=1 SV=3	[more]
SBT16_ARATH	9.8e-34	45.06	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1	[more]
SBT17_ARATH	1.2e-31	43.79	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1	[more]

BLAST of CmoCh13G003540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVY8_CUCSA	1.7e-64	74.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171040 PE=4 SV=1	[more]
A0A0A0LYF1_CUCSA	3.0e-58	66.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G171030 PE=4 SV=1	[more]
A0A0J8BGA7_BETVU	7.0e-47	56.90	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066150 PE=4 S...	[more]
K7MJ92_SOYBN	1.2e-46	58.24	Uncharacterized protein OS=Glycine max GN=GLYMA_17G004100 PE=4 SV=1	[more]
A0A068B924_CICAR	1.2e-46	59.76	Subtilase OS=Cicer arietinum PE=4 SV=1	[more]

BLAST of CmoCh13G003540 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G59810.1	6.3e-47	53.22	Subtilase family protein	[more]
AT2G04160.1	6.3e-39	47.93	Subtilisin-like serine endopeptidase family protein	[more]
AT4G34980.1	5.5e-35	45.06	subtilisin-like serine protease 2	[more]
AT5G67360.1	6.7e-33	43.79	Subtilase family protein	[more]
AT2G05920.1	1.1e-32	43.90	Subtilase family protein	[more]

BLAST of CmoCh13G003540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659128687\|ref\|XP_008464322.1\|	3.7e-65	75.29	PREDICTED: subtilisin-like protease [Cucumis melo]	[more]
gi\|449443664\|ref\|XP_004139597.1\|	2.4e-64	74.12	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
gi\|700209886\|gb\|KGN64982.1\|	2.4e-64	74.12	hypothetical protein Csa_1G171040 [Cucumis sativus]	[more]
gi\|659128619\|ref\|XP_008464289.1\|	1.4e-59	68.24	PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo]	[more]
gi\|778665004\|ref\|XP_011648463.1\|	4.4e-58	66.47	PREDICTED: subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000209	Peptidase_S8/S53_dom
IPR015500	Peptidase_S8_subtilisin-rel

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh13G003540.1	CmoCh13G003540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	GENE3D	G3DSA:3.40.50.200		coord: 24..151 score: 7.4
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 26..103 score: 1.9
IPR000209	Peptidase S8/S53 domain	unknown	SSF52743	Subtilisin-like	coord: 13..137 score: 6.81
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..190 score: 1.4
None	No IPR available	PANTHER	PTHR10795:SF3	SUBFAMILY NOT NAMED	coord: 1..190 score: 1.4

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmoCh13G003540	Cucurbita pepo (Zucchini)	cmocpeB180
CmoCh13G003540	Cucurbita pepo (Zucchini)	cmocpeB204
CmoCh13G003540	Bottle gourd (USVL1VR-Ls)	cmolsiB194
CmoCh13G003540	Cucumber (Gy14) v2	cgybcmoB072
CmoCh13G003540	Cucumber (Chinese Long) v3	cmocucB0239
CmoCh13G003540	Watermelon (97103) v2	cmowmbB192
CmoCh13G003540	Wax gourd	cmowgoB0270
CmoCh13G003540	Cucurbita moschata (Rifu)	cmocmoB167
CmoCh13G003540	Cucumber (Gy14) v1	cgycmoB0880
CmoCh13G003540	Cucurbita maxima (Rimu)	cmacmoB218
CmoCh13G003540	Cucurbita maxima (Rimu)	cmacmoB395
CmoCh13G003540	Wild cucumber (PI 183967)	cmocpiB208
CmoCh13G003540	Cucumber (Chinese Long) v2	cmocuB206
CmoCh13G003540	Watermelon (Charleston Gray)	cmowcgB171
CmoCh13G003540	Watermelon (97103) v1	cmowmB189