CmoCh11G014020.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh11G014020.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Retrotransposon protein, putative, Ty3-gypsy subclass
Location	Cmo_Chr11 : 9861731 .. 9863894 (-)
Sequence length	1389

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTGTGATAATAGGGCTGAAGCAATCCATGACACAGACAAATGGGCAACTGGCACAACTGATCCCGACCTTAGCGCAAAGTGCCAGGCAGACGACCCATCACAACCAGGGAGACGTTGATGCATTAGAAGAAAGTAAGTATTTTTGGGCTTTCAAACGTCGAGACCCCAAAGTATTCGAGGAAAAATCCAAGGACCCTGACGTAGCTGAATTATGGTTGTCGGCTATTGATGAAATTTTTCGACGCATGAAGTGTCTGGAGGAACATAGACTGAGTTGCGTGACCTATTTGTTACGCGAAGATGCTAAACTGTGGTGGCAATCAGCCTCTCGGGCGATTACTGCAGACAAGGATGACATCACCTGGATGCAGTTTAGAAAAGCGTTTCTCCGAAAATACTTCAAACCATTGGTGCGATATAAGAAGCAACGCGAATTCCTAAGCATAGAGCAGGAAAACAGGTCAGTAGAGGAGTATGAACGAGAGTTCACCCGTTTGTCTCGTTTCGCCCCAATAATGGTCTCAACAGAAGTGCTGAAAGTAGAATGTTTTGTTATGGGTTTGCGGCCCGATGTCTGAGGGGCGGTATCAGCTCACGTTTCACCTAACTATGCAACAGCACTCCGACTTGTCGAGACGCTCGATGTTAGGGAATATCCAACAGAAGACATCTAAACCCACTTAAACACGTCGGTTGACCAGAAGAGAAAGAGAGAGCAGACGTCACCTACCTCCTCAAAGGTATCTTGGCACCAGGCCAAGACAAATTTAAACCAAGAGCGCAGGCAACCAAAGGCTTGCCCACAGAACACGCCACGTGACAGACCACGTTGCTCGAACTGTGGGAGACGTCACCTAGGACAGTGCAGGTTTAAGAATAGGGTATGTTTCAACTGTCACTTAGAAGGCCACGTTGTTGCGCACTGCACCCAAGGGAGAGCAACTAGTATCAATAGACCCCCTAGTAACCAACCAAACTCAAACCTGAGGCCAGCTCCGCAACAGGGGCGAATGTTGCTACCACTCGCCAGGAGGCAGAAAACTCCAACACTGCAGTCACAGGTACACTCTCTATTCTTGGGTACTACGCACTCTTTTATCTCCACTCTTTTATCTCCATTAATCTTGTAAAACACGCAAGAGTAGAAGTTGAACCGCTAGGGTACGGGTTGTCTGTGGGTACCCCAGCAGAGGTAAGTATGGAAGCCTTCGAGAGTGTAAAGGATTATCAGTTTTGTGTGTCAAACTACACGATGGATGTGTGATAATAGTCCTAGATATGACAAATTTTGACGTCAACTTAGGGATGGAGTGGTTAGCTAAAACCCATGTATCCATCGATTGCTTCAACAAGGAGGTGGTGTTCAGACCTCCTGGTCAACCAAGCTTCAAGTTCAAGGGGACCAGGGAGGGAACGGTCTCGAGGATAGTCTCGGCATTAAAGGCAAGGAAGATGTTTTCCCAGGGTGCTTGGGGGATATTAGCTCATGTTGTGGAATTAGGGCGAACTGAGGCGAGCATTAACTCAGTGCTAGTAGTGAGAGAATTCGTGGACGTGTTCCCAGAAGATCTCCCGAGCCTACCCCCAGAGCGTGAGATGGAATTCGAAATTGTACTTGAGCCAGGAACAACCCCTATATCAAGAGCCCTATACAGAATGGCCCCTGCTGAACTCAAAGAACTAAAGTTGCAACTTCAAGAGCTACTAAGCAAATGCTTTATACGACCAAGTGTGTCTCCTTGGGGAGCCCCAGTGATATTTGTGAAGAAGAAGGACAACTCTATGCGCTTATACATGGACTATCGAGAACTTAACAAAGTGACAATTAAAAACAAGTATCCTTTACCCCGAATCGATGACCTGTTCGACCAGTTGCAAGGAGCAGTTGTGTTCTCAAAGATTGACTTACGGTCAGGCTACCATCAGTTGAGGATTAAGGAGAGTGATATCTCAAAGACTGCCTTTCGCATAAGATATGGACATTACGAGTTTAGGGTAATGTCGTTTGGCTTGACCAACACTCCAACAGCATTTATGGGGTTGATGAACAAAGTGTTCAGAGAGTTCTTAGACAACTTCGTGATCGTCTTCATCGATGATATCCTTGTGTGTATTCCAAGACTAAGGAACAACTCGAGGAACATCTTCGGAAAGTGCTGA

mRNA sequence

ATGACTGTGATAATAGGGCTGAAGCAATCCATGACACAGACAAATGGGCAACTGGCACAACTGATCCCGACCTTAGCGCAAAGTGCCAGGCAGACGACCCATCACAACCAGGGAGACGTTGATGCATTAGAAGAAAGTAAGTATTTTTGGGCTTTCAAACGTCGAGACCCCAAAGTATTCGAGGAAAAATCCAAGGACCCTGACGTAGCTGAATTATGGTTGTCGGCTATTGATGAAATTTTTCGACGCATGAAGTGTCTGGAGGAACATAGACTGAGTTGCGTGACCTATTTGTTACGCGAAGATGCTAAACTGTGGTGGCAATCAGCCTCTCGGGCGATTACTGCAGACAAGGATGACATCACCTGGATGCAGTTTAGAAAAGCGTTTCTCCGAAAATACTTCAAACCATTGGTGCGATATAAGAAGCAACGCGAATTCCTAAGCATAGAGCAGGAAAACAGCTCCGCAACAGGGGCGAATGTTGCTACCACTCGCCAGGAGGCAGAAAACTCCAACACTGCAGTCACAGGGATGGAGTGGTTAGCTAAAACCCATGTATCCATCGATTGCTTCAACAAGGAGGTGGTGTTCAGACCTCCTGGTCAACCAAGCTTCAAGTTCAAGGGGACCAGGGAGGGAACGGTCTCGAGGATAGTCTCGGCATTAAAGGCAAGGAAGATGTTTTCCCAGGGTGCTTGGGGGATATTAGCTCATGTTGTGGAATTAGGGCGAACTGAGGCGAGCATTAACTCAGTGCTAGTAGTGAGAGAATTCGTGGACGTGTTCCCAGAAGATCTCCCGAGCCTACCCCCAGAGCGTGAGATGGAATTCGAAATTGTACTTGAGCCAGGAACAACCCCTATATCAAGAGCCCTATACAGAATGGCCCCTGCTGAACTCAAAGAACTAAAGTTGCAACTTCAAGAGCTACTAAGCAAATGCTTTATACGACCAAGTGTGTCTCCTTGGGGAGCCCCAGTGATATTTGTGAAGAAGAAGGACAACTCTATGCGCTTATACATGGACTATCGAGAACTTAACAAAGTGACAATTAAAAACAAGTATCCTTTACCCCGAATCGATGACCTGTTCGACCAGTTGCAAGGAGCAGTTGTGTTCTCAAAGATTGACTTACGGTCAGGCTACCATCAGTTGAGGATTAAGGAGAGTGATATCTCAAAGACTGCCTTTCGCATAAGATATGGACATTACGAGTTTAGGGTAATGTCGTTTGGCTTGACCAACACTCCAACAGCATTTATGGGGTTGATGAACAAAGTGTTCAGAGAGTTCTTAGACAACTTCGTGATCGTCTTCATCGATGATATCCTTGTGTGTATTCCAAGACTAAGGAACAACTCGAGGAACATCTTCGGAAAGTGCTGA

Coding sequence (CDS)

ATGACTGTGATAATAGGGCTGAAGCAATCCATGACACAGACAAATGGGCAACTGGCACAACTGATCCCGACCTTAGCGCAAAGTGCCAGGCAGACGACCCATCACAACCAGGGAGACGTTGATGCATTAGAAGAAAGTAAGTATTTTTGGGCTTTCAAACGTCGAGACCCCAAAGTATTCGAGGAAAAATCCAAGGACCCTGACGTAGCTGAATTATGGTTGTCGGCTATTGATGAAATTTTTCGACGCATGAAGTGTCTGGAGGAACATAGACTGAGTTGCGTGACCTATTTGTTACGCGAAGATGCTAAACTGTGGTGGCAATCAGCCTCTCGGGCGATTACTGCAGACAAGGATGACATCACCTGGATGCAGTTTAGAAAAGCGTTTCTCCGAAAATACTTCAAACCATTGGTGCGATATAAGAAGCAACGCGAATTCCTAAGCATAGAGCAGGAAAACAGCTCCGCAACAGGGGCGAATGTTGCTACCACTCGCCAGGAGGCAGAAAACTCCAACACTGCAGTCACAGGGATGGAGTGGTTAGCTAAAACCCATGTATCCATCGATTGCTTCAACAAGGAGGTGGTGTTCAGACCTCCTGGTCAACCAAGCTTCAAGTTCAAGGGGACCAGGGAGGGAACGGTCTCGAGGATAGTCTCGGCATTAAAGGCAAGGAAGATGTTTTCCCAGGGTGCTTGGGGGATATTAGCTCATGTTGTGGAATTAGGGCGAACTGAGGCGAGCATTAACTCAGTGCTAGTAGTGAGAGAATTCGTGGACGTGTTCCCAGAAGATCTCCCGAGCCTACCCCCAGAGCGTGAGATGGAATTCGAAATTGTACTTGAGCCAGGAACAACCCCTATATCAAGAGCCCTATACAGAATGGCCCCTGCTGAACTCAAAGAACTAAAGTTGCAACTTCAAGAGCTACTAAGCAAATGCTTTATACGACCAAGTGTGTCTCCTTGGGGAGCCCCAGTGATATTTGTGAAGAAGAAGGACAACTCTATGCGCTTATACATGGACTATCGAGAACTTAACAAAGTGACAATTAAAAACAAGTATCCTTTACCCCGAATCGATGACCTGTTCGACCAGTTGCAAGGAGCAGTTGTGTTCTCAAAGATTGACTTACGGTCAGGCTACCATCAGTTGAGGATTAAGGAGAGTGATATCTCAAAGACTGCCTTTCGCATAAGATATGGACATTACGAGTTTAGGGTAATGTCGTTTGGCTTGACCAACACTCCAACAGCATTTATGGGGTTGATGAACAAAGTGTTCAGAGAGTTCTTAGACAACTTCGTGATCGTCTTCATCGATGATATCCTTGTGTGTATTCCAAGACTAAGGAACAACTCGAGGAACATCTTCGGAAAGTGCTGA

Homology

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BLAST of CmoCh11G014020.1 vs. Swiss-Prot
Match: YI31B_YEAST (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY3B-I PE=3 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 2.6e-32
Identity = 76/192 (39.58%), Postives = 113/192 (58.85%), Query Frame = 1


Query: 255 VVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYRMAPAELKELKLQLQELLSK 314
           ++R  +   P D+ ++P + ++E    ++PG        Y +     +E+   +Q+LL  
Sbjct: 593 IIRNDLPPRPADINNIPVKHDIE----IKPGARLPRLQPYHVTEKNEQEINKIVQKLLDN 652

Query: 315 CFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNKYPLPRIDDLFDQLQGAVVF 374
            FI PS SP  +PV+ V KKD + RL +DYR LNK TI + +PLPRID+L  ++  A +F
Sbjct: 653 KFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIF 712

Query: 375 SKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLTNTPTAFMGLMNKVFREFLD 434
           + +DL SGYHQ+ ++  D  KTAF    G YE+ VM FGL N P+ F   M   FR+   
Sbjct: 713 TTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRDL-- 772

Query: 435 NFVIVFIDDILV 447
            FV V++DDIL+
Sbjct: 773 RFVNVYLDDILI 778

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BLAST of CmoCh11G014020.1 vs. Swiss-Prot
Match: YG31B_YEAST (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 141.4 bits (355), Expect = 2.6e-32
Identity = 76/192 (39.58%), Postives = 113/192 (58.85%), Query Frame = 1


Query: 255 VVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYRMAPAELKELKLQLQELLSK 314
           ++R  +   P D+ ++P + ++E    ++PG        Y +     +E+   +Q+LL  
Sbjct: 567 IIRNDLPPRPADINNIPVKHDIE----IKPGARLPRLQPYHVTEKNEQEINKIVQKLLDN 626

Query: 315 CFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNKYPLPRIDDLFDQLQGAVVF 374
            FI PS SP  +PV+ V KKD + RL +DYR LNK TI + +PLPRID+L  ++  A +F
Sbjct: 627 KFIVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIF 686

Query: 375 SKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLTNTPTAFMGLMNKVFREFLD 434
           + +DL SGYHQ+ ++  D  KTAF    G YE+ VM FGL N P+ F   M   FR+   
Sbjct: 687 TTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRDL-- 746

Query: 435 NFVIVFIDDILV 447
            FV V++DDIL+
Sbjct: 747 RFVNVYLDDILI 752

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BLAST of CmoCh11G014020.1 vs. Swiss-Prot
Match: RRPO_OENBE (RNA-directed DNA polymerase homolog OS=Oenothera berteroana PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.8e-31
Identity = 65/110 (59.09%), Postives = 83/110 (75.45%), Query Frame = 1


Query: 337 SMRLYMDYRELNKVTIKNKYPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKT 396
           S+R+ +DYR L KVTIKNKYP+PR+DDLFD+L  A  F+K+DLRSGY Q+RI + D  KT
Sbjct: 5   SLRMCIDYRALTKVTIKNKYPIPRVDDLFDRLAQATWFTKLDLRSGYWQVRIAKGDEPKT 64

Query: 397 AFRIRYGHYEFRVMSFGLTNTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
               RYG +EFRVM FGLTN    F  LMN V  E+LD+FV+V++DD++V
Sbjct: 65  TCVTRYGSFEFRVMPFGLTNALATFCNLMNNVLYEYLDHFVVVYLDDLVV 114

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BLAST of CmoCh11G014020.1 vs. Swiss-Prot
Match: POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster GN=pol PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.3e-30
Identity = 67/177 (37.85%), Postives = 104/177 (58.76%), Query Frame = 1


Query: 287 TPISRALYRMAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKD-----NSMRLY 346
           +PI    Y +A     E++ Q+QE+L++  IR S SP+ +P   V KK      N  R+ 
Sbjct: 205 SPIYSKQYPLAQTHEIEVENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVV 264

Query: 347 MDYRELNKVTIKNKYPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIR 406
           +DYR+LN++TI ++YP+P +D++  +L     F+ IDL  G+HQ+ + E  ISKTAF  +
Sbjct: 265 IDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTK 324

Query: 407 YGHYEFRVMSFGLTNTPTAFMGLMNKVFREFLDNFVIVFIDDILVCIPRLRNNSRNI 459
            GHYE+  M FGL N P  F   MN + R  L+   +V++DDI++    L  +  +I
Sbjct: 325 SGHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSI 381

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BLAST of CmoCh11G014020.1 vs. Swiss-Prot
Match: TF29_SCHPO (Transposon Tf2-9 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=Tf2-9 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.0e-28
Identity = 65/194 (33.51%), Postives = 114/194 (58.76%), Query Frame = 1


Query: 255 VVREFVDVFPE-DLPSLP-PEREMEFEIVLEPGTTPISRALYRMAPAELKELKLQLQELL 314
           + +EF D+  E +   LP P + +EFE+ L      +    Y + P +++ +  ++ + L
Sbjct: 377 IYKEFKDITAETNTEKLPKPIKGLEFEVELTQENYRLPIRNYPLPPGKMQAMNDEINQGL 436

Query: 315 SKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNKYPLPRIDDLFDQLQGAV 374
               IR S +    PV+FV KK+ ++R+ +DY+ LNK    N YPLP I+ L  ++QG+ 
Sbjct: 437 KSGIIRESKAINACPVMFVPKKEGTLRMVVDYKPLNKYVKPNIYPLPLIEQLLAKIQGST 496

Query: 375 VFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLTNTPTAFMGLMNKVFREF 434
           +F+K+DL+S YH +R+++ D  K AFR   G +E+ VM +G++  P  F   +N +  E 
Sbjct: 497 IFTKLDLKSAYHLIRVRKGDEHKLAFRCPRGVFEYLVMPYGISTAPAHFQYFINTILGEA 556

Query: 435 LDNFVIVFIDDILV 447
            ++ V+ ++DDIL+
Sbjct: 557 KESHVVCYMDDILI 570

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BLAST of CmoCh11G014020.1 vs. TrEMBL
Match: M5WLY8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021229mg PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 5.4e-98
Identity = 175/271 (64.58%), Postives = 212/271 (78.23%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WLA+   S+DCF KEVVF   GQP   F G R    S ++SA+ A+++  +G  G
Sbjct: 153 ILGMDWLARHRASVDCFRKEVVFHSLGQPEVTFYGERRVLPSCLISAMTAKRLLRKGCSG 212

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            +AHV++       +  + V+++F DVFPEDLP LPP RE+EF I L PGT PIS+A YR
Sbjct: 213 YIAHVIDTRDNGLRLEDIPVIQDFPDVFPEDLPGLPPHREIEFVIELAPGTNPISQAPYR 272

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAEL+ELK QLQEL+ K FIRPS SPWGAPV+FVKKKD +MRL +DYR+LNK+T++N+
Sbjct: 273 MAPAELRELKTQLQELVDKGFIRPSFSPWGAPVLFVKKKDGTMRLCVDYRQLNKITVRNR 332

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA VFSKIDLRSGYHQLR++E D+ KTAFR RYGHYEF VM FGLT
Sbjct: 333 YPLPRIDDLFDQLKGAKVFSKIDLRSGYHQLRVREEDMPKTAFRTRYGHYEFLVMPFGLT 392

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VFR +LD FVIVFIDDILV
Sbjct: 393 NAPAAFMDLMNRVFRRYLDRFVIVFIDDILV 423

[Close]

BLAST of CmoCh11G014020.1 vs. TrEMBL
Match: M5WXB0_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014973mg PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.7e-97
Identity = 172/271 (63.47%), Postives = 213/271 (78.60%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WLA+   S+DCF KEVVFR PG+    F G R    S ++SA+ A+++  +G  G
Sbjct: 256 ILGMDWLARHRASVDCFRKEVVFRSPGRHEVTFYGERRVLPSCLISAMTAKRLLRKGCSG 315

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            +AHV++       +  + ++++F DVFPEDLP +PP+RE+EF I L PGT PIS+A YR
Sbjct: 316 YIAHVIDTRDNGLRLEDIPIIQDFPDVFPEDLPGVPPQREIEFVIELAPGTNPISQAPYR 375

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAEL+ELK QLQEL+ K FI PS SPWGAPV+FVKKKD +MRL +DYR+LNK+T++N+
Sbjct: 376 MAPAELRELKTQLQELVDKGFICPSFSPWGAPVLFVKKKDGTMRLCVDYRQLNKITVRNR 435

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA VFSKIDLRSGYHQLR++E D+ KTAFR RYGHYEF VM FGLT
Sbjct: 436 YPLPRIDDLFDQLKGAKVFSKIDLRSGYHQLRVREEDVPKTAFRTRYGHYEFLVMPFGLT 495

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VFR +LD FVIVFIDDILV
Sbjct: 496 NVPAAFMDLMNRVFRRYLDRFVIVFIDDILV 526

[Close]

BLAST of CmoCh11G014020.1 vs. TrEMBL
Match: A0A061EEG7_THECC (DNA/RNA polymerases superfamily protein OS=Theobroma cacao GN=TCM_018243 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 4.6e-97
Identity = 179/271 (66.05%), Postives = 210/271 (77.49%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM WL+  H S+DC++K V F  PG+PSF  +G R    + ++S + AR++  QG  G
Sbjct: 434 ILGMNWLSPCHASVDCYHKLVRFDFPGEPSFSIQGDRSNAPTNLISVISARRLLRQGCIG 493

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            LA V +       +  V VV+EFVDVFPE+LPSLPPERE+EF I L P T PIS   YR
Sbjct: 494 YLAVVKDSQAKIGDVTQVSVVKEFVDVFPEELPSLPPEREVEFCIDLIPDTRPISIPPYR 553

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAELKELK QL++LL K FIRPSVSPWGAPV+FVKKKD S+RL +DYR+LNKVT+KNK
Sbjct: 554 MAPAELKELKDQLEDLLDKGFIRPSVSPWGAPVLFVKKKDGSLRLCIDYRQLNKVTVKNK 613

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQLQGA  FSKIDLRSGYHQLRI+  DI KTAFR RYGHYEF VMSFGLT
Sbjct: 614 YPLPRIDDLFDQLQGAQCFSKIDLRSGYHQLRIRNEDIPKTAFRTRYGHYEFLVMSFGLT 673

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VF+ +LD FV+VFIDDIL+
Sbjct: 674 NAPAAFMDLMNRVFKPYLDKFVVVFIDDILI 704

[Close]

BLAST of CmoCh11G014020.1 vs. TrEMBL
Match: A0A061G943_THECC (Uncharacterized protein OS=Theobroma cacao GN=TCM_027940 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 6.0e-97
Identity = 178/271 (65.68%), Postives = 210/271 (77.49%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM WL+  H S+DC++K V F  PG+PSF  +G R    + ++S + AR++  QG  G
Sbjct: 392 ILGMNWLSPCHASVDCYHKLVRFDFPGEPSFSIQGDRSNAPTNLISVISARRLLRQGCMG 451

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            LA + +       +  V VV+EFVDVFPE+LPSLPPERE+EF I L P T PIS   YR
Sbjct: 452 YLAVLKDSQAKIGDVTQVSVVKEFVDVFPEELPSLPPEREVEFCIDLIPDTRPISIPPYR 511

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAELKELK QL++LL K FIRPSVSPWGAPV+FVKKKD S+RL +DYR+LNKVT+KNK
Sbjct: 512 MAPAELKELKDQLEDLLDKGFIRPSVSPWGAPVLFVKKKDGSLRLCIDYRQLNKVTVKNK 571

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQLQGA  FSKIDLRSGYHQLRI+  DI KTAFR RYGHYEF VMSFGLT
Sbjct: 572 YPLPRIDDLFDQLQGAQCFSKIDLRSGYHQLRIRNEDIPKTAFRTRYGHYEFLVMSFGLT 631

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VF+ +LD FV+VFIDDIL+
Sbjct: 632 NAPAAFMDLMNRVFKPYLDKFVVVFIDDILI 662

[Close]

BLAST of CmoCh11G014020.1 vs. TrEMBL
Match: M5X787_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022673mg PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 2.3e-96
Identity = 175/271 (64.58%), Postives = 211/271 (77.86%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WLA+   S+DCF KEVVFR PG+P   F G R    S ++SA+ A+++  +G  G
Sbjct: 524 ILGMDWLARHRASVDCFRKEVVFRSPGRPEVTFYGKRRVLPSYLISAMTAKRLLRKGCSG 583

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            +AHV++    E  +  + VV++F DVFPEDLP LPP RE+EF I L PGT  IS+A YR
Sbjct: 584 YIAHVIDTRDNELRLEDIPVVQDFSDVFPEDLPGLPPHREIEFVIELAPGTNLISQAPYR 643

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAEL+ELK QLQEL+ K FIRPS SPWGA V+FVKKKD +MRL +DYR+LNK+T++N+
Sbjct: 644 MAPAELRELKTQLQELVDKGFIRPSFSPWGALVLFVKKKDGTMRLCIDYRQLNKITVQNR 703

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA VFSKIDLRSGYHQL  +E D+ KTAFR RYGHYEF VM FGLT
Sbjct: 704 YPLPRIDDLFDQLKGAKVFSKIDLRSGYHQLWGREEDVPKTAFRTRYGHYEFLVMPFGLT 763

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VFR +LD FVIVFIDDILV
Sbjct: 764 NAPAAFMDLMNRVFRRYLDRFVIVFIDDILV 794

[Close]

BLAST of CmoCh11G014020.1 vs. NCBI nr
Match: gi|985458836|ref|XP_015387942.1| (PREDICTED: uncharacterized protein LOC107177914 [Citrus sinensis])

HSP 1 Score: 376.7 bits (966), Expect = 5.8e-101
Identity = 181/271 (66.79%), Postives = 217/271 (80.07%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WL   HVSIDCF KE++FR PG+  F F+G  +   + ++S +KA KM  +G  G
Sbjct: 455 ILGMDWLGPYHVSIDCFAKEIIFRLPGEEEFHFQGNHKSHKA-LISMVKAMKMLKKGCEG 514

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            LA++V      A +  + +VREF+DVFPEDLP LPP+RE+EF I L PGTTPIS+A YR
Sbjct: 515 FLAYIVADHPDGACLEDIPIVREFIDVFPEDLPGLPPDREVEFTIELVPGTTPISKAPYR 574

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAP ELKELK+QLQELL K FIRPSVSPWGAPV+FVKKKD SMRL +DYR+LN VT+KNK
Sbjct: 575 MAPIELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRQLNMVTVKNK 634

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA +FSKIDLRSGYHQL+I+  D+SKTAFR RYGHYEF VM FGLT
Sbjct: 635 YPLPRIDDLFDQLRGAAIFSKIDLRSGYHQLKIRSEDVSKTAFRTRYGHYEFLVMPFGLT 694

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN++F+ +LD FVIVFIDDIL+
Sbjct: 695 NAPAAFMDLMNRIFQPYLDQFVIVFIDDILI 724

[Close]

BLAST of CmoCh11G014020.1 vs. NCBI nr
Match: gi|985452009|ref|XP_015386531.1| (PREDICTED: uncharacterized protein LOC107177356 [Citrus sinensis])

HSP 1 Score: 376.7 bits (966), Expect = 5.8e-101
Identity = 181/271 (66.79%), Postives = 217/271 (80.07%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WL   HVSIDCF KE++FR PG+  F F+G  +   + ++S +KA KM  +G  G
Sbjct: 470 ILGMDWLGPYHVSIDCFAKEIIFRLPGEEEFHFQGNHKSHKA-LISMVKAMKMLKKGCEG 529

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            LA++V      A +  + +VREF+DVFPEDLP LPP+RE+EF I L PGTTPIS+A YR
Sbjct: 530 FLAYIVADHPDGACLEDIPIVREFIDVFPEDLPGLPPDREVEFTIELVPGTTPISKAPYR 589

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAP ELKELK+QLQELL K FIRPSVSPWGAPV+FVKKKD SMRL +DYR+LN VT+KNK
Sbjct: 590 MAPIELKELKVQLQELLDKGFIRPSVSPWGAPVLFVKKKDGSMRLCIDYRQLNMVTVKNK 649

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA +FSKIDLRSGYHQL+I+  D+SKTAFR RYGHYEF VM FGLT
Sbjct: 650 YPLPRIDDLFDQLRGAAIFSKIDLRSGYHQLKIRSEDVSKTAFRTRYGHYEFLVMPFGLT 709

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN++F+ +LD FVIVFIDDIL+
Sbjct: 710 NAPAAFMDLMNRIFQPYLDQFVIVFIDDILI 739

[Close]

BLAST of CmoCh11G014020.1 vs. NCBI nr
Match: gi|645233554|ref|XP_008223402.1| (PREDICTED: uncharacterized protein LOC103323205 [Prunus mume])

HSP 1 Score: 373.2 bits (957), Expect = 6.4e-100
Identity = 180/283 (63.60%), Postives = 219/283 (77.39%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WL K   S+DCF KEVV R PGQP   F G R    S  +SA++A+++F++G  G
Sbjct: 202 ILGMDWLEKHRASVDCFRKEVVSRSPGQPEVVFHGERRILPSCFISAIRAKRLFNKGCVG 261

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            LAH+++  R+  ++  + VV EF DVFP+DLP LPP+RE EF I L PGT PI +A YR
Sbjct: 262 YLAHIIDTQRSTLNLEDIPVVCEFSDVFPDDLPGLPPQRETEFTIELLPGTNPIHQAPYR 321

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAEL+ELK QLQEL+   FIRPSVSPWGAPV+FV+KKD SMRL +DYR+LNKVT++N+
Sbjct: 322 MAPAELRELKTQLQELVDLGFIRPSVSPWGAPVLFVRKKDGSMRLCIDYRQLNKVTVRNR 381

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA  FSKIDLRSGYHQLR++E DI KTAFR RYGHYEF VM FGLT
Sbjct: 382 YPLPRIDDLFDQLKGAKYFSKIDLRSGYHQLRVREDDIPKTAFRTRYGHYEFLVMPFGLT 441

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILVCIPRLRNNSRNI 459
           N P AFM LMN+VFR +LD FVIVFIDDILV    L  + +++
Sbjct: 442 NAPAAFMDLMNRVFRPYLDRFVIVFIDDILVYSRTLEGHKKHL 484

[Close]

BLAST of CmoCh11G014020.1 vs. NCBI nr
Match: gi|1009116226|ref|XP_015874658.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC107411564, partial [Ziziphus jujuba])

HSP 1 Score: 368.6 bits (945), Expect = 1.6e-98
Identity = 178/271 (65.68%), Postives = 215/271 (79.34%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WLA+ H S+DCF+KEV FR PG P   F G     + R++S + A+K+ ++G  G
Sbjct: 218 ILGMDWLARNHASVDCFSKEVTFRRPGLPEVVFHGGIGRPLPRLISTITAKKLLNKGCQG 277

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            LAHV++   +   +  + VVR+F +VFPE+LP LPPERE++F I L PGT PIS   YR
Sbjct: 278 YLAHVIDTRVSGVRLEDMPVVRDFPNVFPEELPGLPPEREVDFPIELIPGTVPISLPPYR 337

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAP EL+ELK+QLQ+L+ K FIRPS+SPWGAPV+FVKKKD S+RL +DYR+LNKVTI N+
Sbjct: 338 MAPTELRELKVQLQDLVDKGFIRPSISPWGAPVLFVKKKDGSLRLCIDYRQLNKVTIPNR 397

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRID LFDQLQGA VFSKIDLRSGYHQLRI+ESDI KTAFR RYGHYEF VMSFGLT
Sbjct: 398 YPLPRIDYLFDQLQGAKVFSKIDLRSGYHQLRIRESDIPKTAFRTRYGHYEFLVMSFGLT 457

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VFR +LD FVIVFIDDIL+
Sbjct: 458 NAPAAFMDLMNRVFRPYLDRFVIVFIDDILI 488

[Close]

BLAST of CmoCh11G014020.1 vs. NCBI nr
Match: gi|595885005|ref|XP_007213082.1| (hypothetical protein PRUPE_ppa021229mg [Prunus persica])

HSP 1 Score: 366.3 bits (939), Expect = 7.8e-98
Identity = 175/271 (64.58%), Postives = 212/271 (78.23%), Query Frame = 1


Query: 176 VTGMEWLAKTHVSIDCFNKEVVFRPPGQPSFKFKGTREGTVSRIVSALKARKMFSQGAWG 235
           + GM+WLA+   S+DCF KEVVF   GQP   F G R    S ++SA+ A+++  +G  G
Sbjct: 153 ILGMDWLARHRASVDCFRKEVVFHSLGQPEVTFYGERRVLPSCLISAMTAKRLLRKGCSG 212

Query: 236 ILAHVVELGRTEASINSVLVVREFVDVFPEDLPSLPPEREMEFEIVLEPGTTPISRALYR 295
            +AHV++       +  + V+++F DVFPEDLP LPP RE+EF I L PGT PIS+A YR
Sbjct: 213 YIAHVIDTRDNGLRLEDIPVIQDFPDVFPEDLPGLPPHREIEFVIELAPGTNPISQAPYR 272

Query: 296 MAPAELKELKLQLQELLSKCFIRPSVSPWGAPVIFVKKKDNSMRLYMDYRELNKVTIKNK 355
           MAPAEL+ELK QLQEL+ K FIRPS SPWGAPV+FVKKKD +MRL +DYR+LNK+T++N+
Sbjct: 273 MAPAELRELKTQLQELVDKGFIRPSFSPWGAPVLFVKKKDGTMRLCVDYRQLNKITVRNR 332

Query: 356 YPLPRIDDLFDQLQGAVVFSKIDLRSGYHQLRIKESDISKTAFRIRYGHYEFRVMSFGLT 415
           YPLPRIDDLFDQL+GA VFSKIDLRSGYHQLR++E D+ KTAFR RYGHYEF VM FGLT
Sbjct: 333 YPLPRIDDLFDQLKGAKVFSKIDLRSGYHQLRVREEDMPKTAFRTRYGHYEFLVMPFGLT 392

Query: 416 NTPTAFMGLMNKVFREFLDNFVIVFIDDILV 447
           N P AFM LMN+VFR +LD FVIVFIDDILV
Sbjct: 393 NAPAAFMDLMNRVFRRYLDRFVIVFIDDILV 423

The following BLAST results are available for this feature:

BLAST of CmoCh11G014020.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
YI31B_YEAST	2.6e-32	39.58	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
YG31B_YEAST	2.6e-32	39.58	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
RRPO_OENBE	2.8e-31	59.09	RNA-directed DNA polymerase homolog OS=Oenothera berteroana PE=4 SV=1	[more]
POL2_DROME	5.3e-30	37.85	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
TF29_SCHPO	5.0e-28	33.51	Transposon Tf2-9 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]

BLAST of CmoCh11G014020.1 vs. TrEMBL


Match Name	E-value	Identity	Description
M5WLY8_PRUPE	5.4e-98	64.58	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021229mg PE=4 SV=1	[more]
M5WXB0_PRUPE	2.7e-97	63.47	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014973mg PE=4 S...	[more]
A0A061EEG7_THECC	4.6e-97	66.05	DNA/RNA polymerases superfamily protein OS=Theobroma cacao GN=TCM_018243 PE=4 SV...	[more]
A0A061G943_THECC	6.0e-97	65.68	Uncharacterized protein OS=Theobroma cacao GN=TCM_027940 PE=4 SV=1	[more]
M5X787_PRUPE	2.3e-96	64.58	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022673mg PE=4 SV=1	[more]

BLAST of CmoCh11G014020.1 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmoCh11G014020.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|985458836\|ref\|XP_015387942.1\|	5.8e-101	66.79	PREDICTED: uncharacterized protein LOC107177914 [Citrus sinensis]	[more]
gi\|985452009\|ref\|XP_015386531.1\|	5.8e-101	66.79	PREDICTED: uncharacterized protein LOC107177356 [Citrus sinensis]	[more]
gi\|645233554\|ref\|XP_008223402.1\|	6.4e-100	63.60	PREDICTED: uncharacterized protein LOC103323205 [Prunus mume]	[more]
gi\|1009116226\|ref\|XP_015874658.1\|	1.6e-98	65.68	PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC107411564, partial [Z...	[more]
gi\|595885005\|ref\|XP_007213082.1\|	7.8e-98	64.58	hypothetical protein PRUPE_ppa021229mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000477	RT_dom
IPR005162	Retrotrans_gag_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh11G014020	CmoCh11G014020	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh11G014020.1	CmoCh11G014020.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G014020.1.CDS.3	CmoCh11G014020.1.CDS.3	CDS
CmoCh11G014020.1.CDS.2	CmoCh11G014020.1.CDS.2	CDS
CmoCh11G014020.1.CDS.1	CmoCh11G014020.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G014020.1.exon.3	CmoCh11G014020.1.exon.3	exon
CmoCh11G014020.1.exon.2	CmoCh11G014020.1.exon.2	exon
CmoCh11G014020.1.exon.1	CmoCh11G014020.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 331..450 score: 1.9
IPR000477	Reverse transcriptase domain	PROFILE	PS50878	RT_POL	coord: 312..462 score:
IPR005162	Retrotransposon gag domain	PFAM	PF03732	Retrotrans_gag	coord: 95..157 score: 8.
None	No IPR available	GENE3D	G3DSA:3.10.10.10		coord: 280..425 score: 3.9
None	No IPR available	PANTHER	PTHR24559	FAMILY NOT NAMED	coord: 324..446 score: 3.6
None	No IPR available	PANTHER	PTHR24559:SF207	SUBFAMILY NOT NAMED	coord: 324..446 score: 3.6
None	No IPR available	unknown	SSF56672	DNA/RNA polymerases	coord: 256..449 score: 4.56