CmoCh11G013580.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh11G013580.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Calcium-binding protein
Location	Cmo_Chr11 : 9443657 .. 9444211 (-)
Sequence length	555

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATCAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCTGATCTCAAAGCACGAATACAGGGGAGTTCTGAAGGCGCTGGGGAGAGGGAACTCGATGGAGGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGGGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

mRNA sequence

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATCAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCTGATCTCAAAGCACGAATACAGGGGAGTTCTGAAGGCGCTGGGGAGAGGGAACTCGATGGAGGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGGGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Coding sequence (CDS)

ATGCCGATGAACCCAAATCTCCTCGACCTGCAATACAGAGTCTCCAAGAAAAAGTTCCTCCGGGAGCCCTCCCGACTGTTCTCCTTCAAGGACCGGCAGAGCTCCGGCGTCCGGCGGGCTCTGCAACCGAGCACGGAGGAGATCAAGCAGATCTTCAACAAGTTCGACACGAACAGAGACGGGCTGATCTCAAAGCACGAATACAGGGGAGTTCTGAAGGCGCTGGGGAGAGGGAACTCGATGGAGGAGGTGCAGAAGATTTTCAGGGCGGTGGATACCGACGGCGATGGGTACATAAACTTGAATGAGTTCATGGAGGTTCACCGGAGTGGGGACGGAGTTCAGGCGAGAGATGTGGAATTTGCTTTTAAGACGTTTGATTTGAATGGGGATCGGAAGATTAGTGCAGAGGAAATTATGAGAGTGTTGAGGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGCGACGGCGACGGTATGGTGGACATTGATGAGTTCATGACTATGATGACTCGCTCTGTGATGTAA

Homology

[Close]

BLAST of CmoCh11G013580.1 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.1e-50
Identity = 97/182 (53.30%), Postives = 142/182 (78.02%), Query Frame = 1


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARD 123
           +DG IS+ EY+ VL+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++   G+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEE+M VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of CmoCh11G013580.1 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-28
Identity = 66/150 (44.00%), Postives = 96/150 (64.00%), Query Frame = 1


Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDT 93
           S  V R    S E+IK++F +FD N DG IS  E + V+ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEVHR----SGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEK 153
           DG+G+I+L+EF+ + +    S +    RD++ AF  +DL+ + +ISA E+  V++ LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmoCh11G013580.1 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 1.3e-27
Identity = 63/152 (41.45%), Postives = 100/152 (65.79%), Query Frame = 1


Query: 35  SGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTD 94
           +GV R+   S ++IK++F +FD N DG IS  E + V++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHRSGDGV------QARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGE 154
           G+G+I+L+EF+ + + G G          D++ AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CmoCh11G013580.1 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.2e-27
Identity = 64/137 (46.72%), Postives = 89/137 (64.96%), Query Frame = 1


Query: 46  EEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFM 105
           EE+K++F++FD+N DG IS  E  GV KA+G   +  E+ ++   VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CmoCh11G013580.1 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.7e-27
Identity = 60/144 (41.67%), Postives = 89/144 (61.81%), Query Frame = 1


Query: 39  RALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGY 98
           R+ +    E++ +F KFD N DG IS  E   ++ +LG     EE++K    +D  GDGY
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  INLNEFMEVHRSG--DGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCR 158
           IN  EF+E++  G        +++ AF  +D++G+  ISAEE+  VLR LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDGMVDIDEFMTMMT 181
           +M+  VD DGDG +D +EF  MMT
Sbjct: 149 KMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CmoCh11G013580.1 vs. TrEMBL
Match: A0A0A0K804_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 3.7e-90
Identity = 166/182 (91.21%), Postives = 178/182 (97.80%), Query Frame = 1


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           G ISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of CmoCh11G013580.1 vs. TrEMBL
Match: M5VNS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 3.3e-62
Identity = 114/178 (64.04%), Postives = 148/178 (83.15%), Query Frame = 1


Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISK 65
           N LD QY +SK+KFLR+PS +FSF+DRQ+SGV  + +P+  E+K++F+KFD+N+DG IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAFKT 125
            EY+ +L+ALG+GN + EV KIFR  D DGDG+IN  EFMEVH+ G G++  D++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           FDLNGD KISAEE+M VL  LGE CS+EDC+RMVRAVD+DGDG+VDIDEFMTMMTRS+
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSM 182

[Close]

BLAST of CmoCh11G013580.1 vs. TrEMBL
Match: B9HS04_POPTR (Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 9.8e-59
Identity = 109/180 (60.56%), Postives = 146/180 (81.11%), Query Frame = 1


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N + L+ QY++S+ KFLR+PSRLFS +DRQSSG+    QP+ +E++Q+F+KFD+N+DG I
Sbjct: 3   NLSFLEFQYKLSRNKFLRKPSRLFSSRDRQSSGMLSTFQPNMKEMRQVFDKFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAF 123
           S+ EY+  L+ALG+GN + EV KIF+ VD DGDG+I+  EF+E  + G G++  D++ AF
Sbjct: 63  SQQEYKDTLRALGQGNMLGEVPKIFQVVDLDGDGFIDFKEFVEAQKKGGGIRTTDIQTAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           +TFD NGD KISAEE+M VLR LGE+CS+EDCRRMV AVD DGDGMV++DEFMTMMTRS+
Sbjct: 123 QTFDSNGDGKISAEEVMEVLRRLGERCSLEDCRRMVNAVDIDGDGMVNMDEFMTMMTRSM 182

[Close]

BLAST of CmoCh11G013580.1 vs. TrEMBL
Match: V4SBR8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029360mg PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.7e-56
Identity = 104/180 (57.78%), Postives = 143/180 (79.44%), Query Frame = 1


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N + LD QY++SK KFLR+PSRLFS +DRQ+S +    Q    E+K++F++FD+N+DG I
Sbjct: 3   NLSFLDFQYKISKNKFLRKPSRLFSSRDRQNSSLLPTFQADRNEMKRVFDRFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAF 123
           S+ EY+ +L+A+G+ + + EV KIF+ VD DGDG+I+  EFME H+ G G++  D++ AF
Sbjct: 63  SQMEYKAILRAIGQASMISEVPKIFQVVDLDGDGFIDFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           +TFD N D KISAEEI+ +LR LGE CS+EDCR+MVRAVD+DGDGMV++DEFMTMMTRS+
Sbjct: 123 RTFDKNDDGKISAEEILEMLRRLGESCSLEDCRKMVRAVDTDGDGMVNMDEFMTMMTRSM 182

[Close]

BLAST of CmoCh11G013580.1 vs. TrEMBL
Match: A0A067F0V1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041237mg PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.7e-56
Identity = 104/180 (57.78%), Postives = 143/180 (79.44%), Query Frame = 1


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N + LD QY++SK KFLR+PSRLFS +DRQ+S +    Q    E+K++F++FD+N+DG I
Sbjct: 3   NLSFLDFQYKISKNKFLRKPSRLFSSRDRQNSSLLPTFQADRNEMKRVFDRFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAF 123
           S+ EY+ +L+A+G+ + + EV KIF+ VD DGDG+I+  EFME H+ G G++  D++ AF
Sbjct: 63  SQMEYKAILRAIGQASMISEVPKIFQVVDLDGDGFIDFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           +TFD N D KISAEEI+ +LR LGE CS+EDCR+MVRAVD+DGDGMV++DEFMTMMTRS+
Sbjct: 123 RTFDKNDDGKISAEEILEMLRRLGESCSLEDCRKMVRAVDTDGDGMVNMDEFMTMMTRSM 182

[Close]

BLAST of CmoCh11G013580.1 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 199.1 bits (505), Expect = 2.3e-51
Identity = 97/182 (53.30%), Postives = 142/182 (78.02%), Query Frame = 1


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRA-----LQPSTEEIKQIFNKFDTN 63
           N + L+LQY++SK K LR+PSR+FS +DRQSSG+         QPS  E++++F++FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARD 123
           +DG IS+ EY+ VL+ALG+  ++E+V KIF+AVD DGDG+I+  EF++ ++   G+++ D
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTM 181
           +  +F TFDLNGD KISAEE+M VL  LGE+CS+EDC RMVRAVD+DGDG+V+++EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of CmoCh11G013580.1 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 127.5 bits (319), Expect = 8.6e-30
Identity = 66/150 (44.00%), Postives = 96/150 (64.00%), Query Frame = 1


Query: 34  SSGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDT 93
           S  V R    S E+IK++F +FD N DG IS  E + V+ AL    S EE + + +  D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYINLNEFMEVHR----SGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEK 153
           DG+G+I+L+EF+ + +    S +    RD++ AF  +DL+ + +ISA E+  V++ LGEK
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSIEDCRRMVRAVDSDGDGMVDIDEFMTMM 180
           CSI+DC+RM+  VDSDGDG VD +EF  MM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmoCh11G013580.1 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 124.4 bits (311), Expect = 7.3e-29
Identity = 63/152 (41.45%), Postives = 100/152 (65.79%), Query Frame = 1


Query: 35  SGVRRALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTD 94
           +GV R+   S ++IK++F +FD N DG IS  E + V++AL    S EE   + +  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVHRSGDGV------QARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGE 154
           G+G+I+L+EF+ + + G G          D++ AF+ +DL+G+ +ISA+E+  V++ LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 181
           KCS++DC++M+  VD DGDG V+ DEF  MM+
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CmoCh11G013580.1 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-28
Identity = 64/137 (46.72%), Postives = 89/137 (64.96%), Query Frame = 1


Query: 46  EEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFM 105
           EE+K++F++FD+N DG IS  E  GV KA+G   +  E+ ++   VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVHRSGDGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSD 165
            + RS     A ++  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD+D
Sbjct: 82  TLCRSSS--SAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 141

Query: 166 GDGMVDIDEFMTMMTRS 183
           GDG V+ +EF  MMT S
Sbjct: 142 GDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CmoCh11G013580.1 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 122.9 bits (307), Expect = 2.1e-28
Identity = 60/144 (41.67%), Postives = 89/144 (61.81%), Query Frame = 1


Query: 39  RALQPSTEEIKQIFNKFDTNRDGLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGY 98
           R+ +    E++ +F KFD N DG IS  E   ++ +LG     EE++K    +D  GDGY
Sbjct: 29  RSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGY 88

Query: 99  INLNEFMEVHRSG--DGVQARDVEFAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCR 158
           IN  EF+E++  G        +++ AF  +D++G+  ISAEE+  VLR LG++CSI +CR
Sbjct: 89  INFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECR 148

Query: 159 RMVRAVDSDGDGMVDIDEFMTMMT 181
           +M+  VD DGDG +D +EF  MMT
Sbjct: 149 KMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CmoCh11G013580.1 vs. NCBI nr
Match: gi|778725125|ref|XP_004137114.2| (PREDICTED: calmodulin-like protein 1 [Cucumis sativus])

HSP 1 Score: 339.0 bits (868), Expect = 5.3e-90
Identity = 166/182 (91.21%), Postives = 178/182 (97.80%), Query Frame = 1


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN L+LQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS+EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           G ISKHEYRG+LKALGRGNSMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of CmoCh11G013580.1 vs. NCBI nr
Match: gi|659109907|ref|XP_008454944.1| (PREDICTED: calmodulin-like protein 1 [Cucumis melo])

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-89
Identity = 166/182 (91.21%), Postives = 177/182 (97.25%), Query Frame = 1


Query: 1   MPMNPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRD 60
           MP+NPN LDLQY VSKKKFLREPSRLFSFKDRQSSG+RRALQPS EEIKQIFNKFDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GLISKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVE 120
           G ISKHEYRG+LKALGRG+SMEEVQKIFRAVD+DGDGYINLNEFMEVHRSG GVQA++VE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 FAFKTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMT 180
           FAFKTFDLNGDRKISAEE+MRVL+GLGEKCSIEDCRRMVRAVDSDGDGMVDI+EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RS 183
           RS
Sbjct: 181 RS 182

[Close]

BLAST of CmoCh11G013580.1 vs. NCBI nr
Match: gi|595798018|ref|XP_007201342.1| (hypothetical protein PRUPE_ppa011990mg [Prunus persica])

HSP 1 Score: 246.1 bits (627), Expect = 4.7e-62
Identity = 114/178 (64.04%), Postives = 148/178 (83.15%), Query Frame = 1


Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISK 65
           N LD QY +SK+KFLR+PS +FSF+DRQ+SGV  + +P+  E+K++F+KFD+N+DG IS+
Sbjct: 5   NFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAFKT 125
            EY+ +L+ALG+GN + EV KIFR  D DGDG+IN  EFMEVH+ G G++  D++ AF+T
Sbjct: 65  PEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAFRT 124

Query: 126 FDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           FDLNGD KISAEE+M VL  LGE CS+EDC+RMVRAVD+DGDG+VDIDEFMTMMTRS+
Sbjct: 125 FDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSM 182

[Close]

BLAST of CmoCh11G013580.1 vs. NCBI nr
Match: gi|645261897|ref|XP_008236514.1| (PREDICTED: calmodulin-like protein 1 [Prunus mume])

HSP 1 Score: 244.2 bits (622), Expect = 1.8e-61
Identity = 112/178 (62.92%), Postives = 147/178 (82.58%), Query Frame = 1


Query: 6   NLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLISK 65
           N LD Q+ +SK+KFLR+PS + SF+DRQ+SG+  + +P+  E+K +F KFD+N+DG IS+
Sbjct: 5   NFLDFQFNLSKRKFLRKPSHVCSFRDRQNSGLSPSFEPNPNEMKIVFEKFDSNKDGKISQ 64

Query: 66  HEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAFKT 125
            EY+ +L+ALG+GN++ EV KIFR  D DGDG+IN  EFMEVH+ G G++  D++ AF+T
Sbjct: 65  EEYKAILRALGKGNTVREVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTMDIQNAFRT 124

Query: 126 FDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 184
           FDLNGD KISAEE+M VLR LGE CS+EDC+RMVRAVD+DGDG+VDIDEFMTMMTRS+
Sbjct: 125 FDLNGDGKISAEEVMEVLRRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRSM 182

[Close]

BLAST of CmoCh11G013580.1 vs. NCBI nr
Match: gi|657963113|ref|XP_008373157.1| (PREDICTED: calmodulin-like protein 1 [Malus domestica])

HSP 1 Score: 242.7 bits (618), Expect = 5.2e-61
Identity = 113/180 (62.78%), Postives = 148/180 (82.22%), Query Frame = 1


Query: 4   NPNLLDLQYRVSKKKFLREPSRLFSFKDRQSSGVRRALQPSTEEIKQIFNKFDTNRDGLI 63
           N N LD QY +SK+KFLR+PS++FSF+DRQ SG+    +P+  E+K++F KFD+N+DG I
Sbjct: 3   NANFLDFQYNLSKRKFLRKPSQMFSFRDRQYSGIAPTFEPNPNEMKKVFEKFDSNKDGKI 62

Query: 64  SKHEYRGVLKALGRGNSMEEVQKIFRAVDTDGDGYINLNEFMEVHRSGDGVQARDVEFAF 123
           S+ EY+ +L+ALG+GN + EV KIF+ VD D DG+IN  EFMEVH+ G G++  D++ AF
Sbjct: 63  SQAEYKAILRALGKGNMIGEVPKIFKFVDLDEDGFINFKEFMEVHKKG-GIRTMDIQNAF 122

Query: 124 KTFDLNGDRKISAEEIMRVLRGLGEKCSIEDCRRMVRAVDSDGDGMVDIDEFMTMMTRSV 183
           + FDLNGD KISAEE+M VLRGL E+C++EDCRRMVRAVD+DGDGMVD+DEFMTMMTRS+
Sbjct: 123 RMFDLNGDGKISAEEVMEVLRGLEERCNLEDCRRMVRAVDTDGDGMVDLDEFMTMMTRSM 181

The following BLAST results are available for this feature:

BLAST of CmoCh11G013580.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML1_ARATH	4.1e-50	53.30	Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1	[more]
CML23_ARATH	1.5e-28	44.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=...	[more]
CML24_ARATH	1.3e-27	41.45	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
CML27_ARATH	2.2e-27	46.72	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=...	[more]
CML25_ARATH	3.7e-27	41.67	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]

BLAST of CmoCh11G013580.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K804_CUCSA	3.7e-90	91.21	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1	[more]
M5VNS5_PRUPE	3.3e-62	64.04	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1	[more]
B9HS04_POPTR	9.8e-59	60.56	Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE...	[more]
V4SBR8_9ROSI	2.7e-56	57.78	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029360mg PE=4 SV=1	[more]
A0A067F0V1_CITSI	2.7e-56	57.78	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041237mg PE=4 SV=1	[more]

BLAST of CmoCh11G013580.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G15680.1	2.3e-51	53.30	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	8.6e-30	44.00	calmodulin like 23	[more]
AT5G37770.1	7.3e-29	41.45	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.2e-28	46.72	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	2.1e-28	41.67	EF hand calcium-binding protein family	[more]

BLAST of CmoCh11G013580.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778725125\|ref\|XP_004137114.2\|	5.3e-90	91.21	PREDICTED: calmodulin-like protein 1 [Cucumis sativus]	[more]
gi\|659109907\|ref\|XP_008454944.1\|	1.2e-89	91.21	PREDICTED: calmodulin-like protein 1 [Cucumis melo]	[more]
gi\|595798018\|ref\|XP_007201342.1\|	4.7e-62	64.04	hypothetical protein PRUPE_ppa011990mg [Prunus persica]	[more]
gi\|645261897\|ref\|XP_008236514.1\|	1.8e-61	62.92	PREDICTED: calmodulin-like protein 1 [Prunus mume]	[more]
gi\|657963113\|ref\|XP_008373157.1\|	5.2e-61	62.78	PREDICTED: calmodulin-like protein 1 [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh11G013580	CmoCh11G013580	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh11G013580.1	CmoCh11G013580.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G013580.1.CDS.1	CmoCh11G013580.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh11G013580.1.exon.1	CmoCh11G013580.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 46..106 score: 6.9E-15coord: 119..179 score: 1.1
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 118..146 score: 0.0024coord: 154..182 score: 0.0063coord: 83..111 score: 2.3E-5coord: 47..75 score: 3.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 150..184 score: 11.194coord: 118..149 score: 12.477coord: 79..114 score: 14.179coord: 43..78 score: 15
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 130..181 score: 9.8E-20coord: 45..129 score: 2.2
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 45..181 score: 5.0
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175 score: -coord: 127..139 score: -coord: 56..68 score: -coord: 92..104 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 42..181 score: 9.7
None	No IPR available	PANTHER	PTHR10891:SF533	SUBFAMILY NOT NAMED	coord: 42..181 score: 9.7