CmoCh01G018570.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh01G018570.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	(3-oxo-delta(4,5)-steroid 5-beta-reductase-like protein) (Progesterone 5-beta-reductase)
Location	Cmo_Chr01 : 13503775 .. 13505613 (-)
Sequence length	1756

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAAAAAAAAAAGGAAGTAATGACCTTCCATAAACATTCGTTTCTGATATTGGTAGATGGTTACATAGGAAAATCCAGTCGGGAGTCCAGCGGTTCGGGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCACCTTATAGCCCTTTCAACACACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCCCTCCTCCCTAAATCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGGTAATTAAAACCCCTAACTCCCTATCTATGCCCCTGTTTCTATTACGTTTCTCTAATCAGCATCTCCGTTCCCAATTTTGCAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGATTGCCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTATGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTGGTAATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATAAGTAATAATCTTTAGAACGTTGTTACATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCT

mRNA sequence

AAAAAAAAAAAAAAAAAAAAGGAAGTAATGACCTTCCATAAACATTCGTTTCTGATATTGGTAGATGGTTACATAGGAAAATCCAGTCGGGAGTCCAGCGGTTCGGGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCACCTTATAGCCCTTTCAACACACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCCCTCCTCCCTAAATCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGATTGCCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTATGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTGGTAATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATAAGTAATAATCTTTAGAACGTTGTTACATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCT

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGA

Homology

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BLAST of CmoCh01G018570.1 vs. Swiss-Prot
Match: VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 2.2e-186
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N+ D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV-DGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIET--VTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

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BLAST of CmoCh01G018570.1 vs. Swiss-Prot
Match: 5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 7.2e-169
Identity = 276/393 (70.23%), Postives = 317/393 (80.66%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARR RP W+ D+PI Y+QCD+SDP+D++AKLS LTDVTHVFYVTW N  TE ENCE N 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FE+      + HDPP+TED+PRL   NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY  ED++ EE+EK  K+ LTW+V RP  IFGFSP+S+MN+VGTLC YAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVEK--KEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           F G K+AW+GYS  SDADLIAE HIW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +    +EEG  L L +LMKGK  VWEEIV+EN L PTKL+DV  WW  D+ L     +DS
Sbjct: 301 VGCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

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BLAST of CmoCh01G018570.1 vs. Swiss-Prot
Match: IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 3.6e-136
Identity = 230/397 (57.93%), Postives = 291/397 (73.30%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQP---RSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWK 60
           MSWWW  +IGA K    ++++    +S++SVALV+GVTGIVG+SLAE+L L DTPGGPWK
Sbjct: 1   MSWWWKRSIGAGKNLPNQNKENGVCKSYKSVALVVGVTGIVGSSLAEVLKLPDTPGGPWK 60

Query: 61  VYGVARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCE 120
           VYGVARRP P W A  P+EYIQCDVSD ++  +KLS L D+TH+FYV+W       E+C+
Sbjct: 61  VYGVARRPCPVWLAKKPVEYIQCDVSDNQETISKLSPLKDITHIFYVSWIGS----EDCQ 120

Query: 121 VNGKMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDS 180
            N  M +N+L SVIPNA NL+H+CLQTG KHY G FE         HD PFTED+PRL+ 
Sbjct: 121 TNATMFKNILNSVIPNASNLQHVCLQTGIKHYFGIFEE--GSKVVPHDSPFTEDLPRLNV 180

Query: 181 PNFYYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGR 240
           PNFY+  ED+L+EE   + K +LTW+V RP  +FGFSP S+MNIV TLC YA ICKHE +
Sbjct: 181 PNFYHDLEDILYEE---TGKNNLTWSVHRPALVFGFSPCSMMNIVSTLCVYATICKHENK 240

Query: 241 PLKFPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAE 300
            L +PG+K++W  Y+   DADL+AE  IW AVDP AKN+  NC+NGDVFKWKH WK LAE
Sbjct: 241 ALVYPGSKNSWNCYADAVDADLVAEHEIWAAVDPKAKNQVLNCNNGDVFKWKHIWKKLAE 300

Query: 301 QFNIEDYGFEEG-ERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASG 360
           +F IE  G+ EG E++ L ELMK K+ VW+EIVK+N L PTKL+++A +W  DIA  +  
Sbjct: 301 EFGIEMVGYVEGKEQVSLAELMKDKDQVWDEIVKKNNLVPTKLKEIAAFWFADIAFCSEN 360

Query: 361 NIDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
            I SMNKSKE GFLGFRNS  SF+S IDK++ ++ +P
Sbjct: 361 LISSMNKSKELGFLGFRNSMKSFVSCIDKMRDYRFIP 388

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BLAST of CmoCh01G018570.1 vs. Swiss-Prot
Match: YJ72_SCHPO (Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC757.02c PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.2e-29
Identity = 108/406 (26.60%), Postives = 174/406 (42.86%), Query Frame = 1


Query: 28  VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDPED 87
           VA+V G TG+ G ++ + L   D       +    +   P     H I+ +     +P+D
Sbjct: 7   VAIVTGATGLNGAAIIKRLSEDDNCKTIHCISRSLKDEYPRKIKHHSIDLLN---EEPKD 66

Query: 88  AEAKLSK--LTDVTHVFYVTWTNLPTEIENCEVNGKMLRNVLRSV-IPNAPNLRHICLQT 147
              K S   +  + + ++  +     E + CEVNG MLRN ++++ + +   LR + L T
Sbjct: 67  IAKKFSLEGVKGINYAYFAAYKEENNEEKLCEVNGNMLRNFVQALELTSIQTLRRVILTT 126

Query: 148 GTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNFYYTQEDLLFEEIEKSQKKDLTWAV 207
           G K Y G     +  P    D    E      +PNFYY QED+L +E    +K D T A+
Sbjct: 127 GLKFY-GLHLGEVRLPMIETDIRVPETFS--GTPNFYYVQEDIL-KEFSNGKKWDYTIAM 186

Query: 208 IRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAWEGYSVVSDADLIAEQH 267
             PN I G S  S MN   T+  YA +C+    P +FPG +  + G+  +S + LIA+  
Sbjct: 187 --PNDICGVSKGSYMNEAFTIALYALVCRELHEPFRFPGNEKFYLGFDDISYSKLIADFQ 246

Query: 268 IWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFNIE--------------DYGFEEGE 327
           +W         E FN  NGD+  W   W  +AE F +E              +       
Sbjct: 247 LWMTFKAECSEEKFNIVNGDIHSWSRTWPKIAEYFGVEVPKNQFATDFTLSTEVTLSTPS 306

Query: 328 RLPLGELMKG-KNAVWEEIVKENQLQ----PTKLED-----------------VANWWHT 387
            + L E   G K+    +I+ +  LQ      K++D                 V  W   
Sbjct: 307 PINLYEKELGIKHTPNSKIINQISLQQWVKQKKVQDAWRTIAEREKLNAHALEVGTWAFC 366

Query: 388 DIALSASGN-IDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           D     + N I SM+K+++ G+  + ++ + F    D++K  K +P
Sbjct: 367 DFLFGRTYNVISSMSKARKLGYTDYYDTFDGFKETFDELKKQKQIP 403

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BLAST of CmoCh01G018570.1 vs. TrEMBL
Match: A0A0B5CYJ8_CUCPE (Putative progesterone 5-beta-reductase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 3.4e-234
Identity = 386/393 (98.22%), Postives = 388/393 (98.73%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570.1 vs. TrEMBL
Match: A0A0A0L8Y0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G610790 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-206
Identity = 338/394 (85.79%), Postives = 360/394 (91.37%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN  TEIENCE N 
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE+II+  +QRHDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLL+EEIE+SQKKDLTWAVIRPN IFGFSPFSLMN+VGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE + V SDADLIAEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGF-EEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
           IE+YGF EEGE L L +LMK K+ VW+EIVKENQLQ TKLE++  WW  D   S SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           SMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]

BLAST of CmoCh01G018570.1 vs. TrEMBL
Match: A0A0S3RB21_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G051100 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 8.8e-190
Identity = 308/393 (78.37%), Postives = 343/393 (87.28%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEED+ PRSFESV LVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDTPRSFESVGLVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEY+QCDVSDP DAE+KLS LTDVT++F+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPPWNADHPIEYVQCDVSDPADAESKLSVLTDVTNIFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLRNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG+LC YAAICKHEG PL+
Sbjct: 181 YYTQEDILFEETAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSLCVYAAICKHEGIPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGT+ AWEGYS  SDADLIAEQHIW AVDPYA+NEAFNCSNGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTRGAWEGYSFSSDADLIAEQHIWAAVDPYARNEAFNCSNGDIFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E MK K  VW+EIV +NQL PTKL++V +WW  D+  S  G +DS
Sbjct: 301 IEEYGFEEGSGLRLSEAMKDKGPVWDEIVSQNQLLPTKLDEVGDWWFVDVIFSGEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNSFISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSFISWIDKSKGYKIVP 389

[Close]

BLAST of CmoCh01G018570.1 vs. TrEMBL
Match: V7AWY5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G070100g PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.1e-189
Identity = 311/393 (79.13%), Postives = 341/393 (86.77%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESV LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WNADHPIEY+QCDVSDP DAE+KLS LTDVTHVF+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG++C YAAICKHEG PL+
Sbjct: 181 YYNQEDILFEESAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSVCVYAAICKHEGVPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWEGYS  SDADLIAEQHIW AVDP A+NEAFNCSNGDVFKWKH WKVLAEQF 
Sbjct: 241 FPGTKGAWEGYSFSSDADLIAEQHIWAAVDPNARNEAFNCSNGDVFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E+MK K  VW+EIV  NQL PTKLE+V +WW  DI  SA G +DS
Sbjct: 301 IEEYGFEEGSHLKLSEVMKDKGPVWDEIVSANQLLPTKLEEVGDWWFVDIIFSAEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNS ISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSLISWIDKCKGYKIVP 389

[Close]

BLAST of CmoCh01G018570.1 vs. TrEMBL
Match: W9SGC8_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_011348 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 4.8e-188
Identity = 307/393 (78.12%), Postives = 338/393 (86.01%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE+E P S++SV LV+GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEENEAPISYQSVGLVVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           V RRPRPNWNADHPIEYIQCD+SDPED   KLS LTDVTH+FYVTW N  TE+ENCEVNG
Sbjct: 61  VGRRPRPNWNADHPIEYIQCDISDPEDTNTKLSTLTDVTHIFYVTWANRATEVENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            M  NVLR++IPNAPNL HICLQTGTKHYIG FE+      + HDPPFTED+PRL+ PNF
Sbjct: 121 AMFHNVLRALIPNAPNLSHICLQTGTKHYIGPFESF--GKIKPHDPPFTEDLPRLEYPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYT ED+L EE+ K  K+ LTW+V RP+TIFGFSP+SLMN++GTLC YAAICKHEGRPLK
Sbjct: 181 YYTLEDVLQEEVRK--KESLTWSVHRPDTIFGFSPYSLMNLIGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK+AW+ YSV SDADLIAEQ IW AVD YAKNEAFNC+NGDVF+WKHFWKVLAEQF 
Sbjct: 241 FPGTKAAWDSYSVASDADLIAEQQIWAAVDLYAKNEAFNCNNGDVFRWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IED+G EEGERL L ELMK K  VWEEIVKENQL+PTKLE+V  WW  D+  S    + S
Sbjct: 301 IEDWGLEEGERLRLTELMKDKGPVWEEIVKENQLEPTKLEEVGVWWFADLMFSGRAPLSS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAYKIVP 389

[Close]

BLAST of CmoCh01G018570.1 vs. TAIR10
Match: AT4G24220.1 (AT4G24220.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 652.9 bits (1683), Expect = 1.3e-187
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N+ D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV-DGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIET--VTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of CmoCh01G018570.1 vs. TAIR10
Match: AT5G58750.1 (AT5G58750.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 237.3 bits (604), Expect = 1.6e-62
Identity = 142/379 (37.47%), Postives = 207/379 (54.62%), Query Frame = 1


Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDP 85
           E+VAL+ GVTG+VG  + + L +S  PG  W++YGVAR P  N +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSK-PG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  EDAEAKLSKLTD-VTHVFYVTWTN-LPTEIENCEVNGK-MLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+HVF+VTW+   P + + C V  K ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDS-PNFYYTQEDLLFEEIEKSQKKDLT 205
           QTG KHY+   E  +          ++E+ PR  S  NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195

Query: 206 WAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAW-EGYSVVSDADLI 265
           W+V RP  + G S  +L N +G+LC Y A+CK+   P  F GT+  W E Y   SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVDPYA--KNEAFNCSNGDVFKWKHFWKVLAEQFNI---EDYGFEEGERLPLG 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  +   E   F+EG     G
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEG--FWFG 315

Query: 326 ELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGN-IDSMNKSKEHGFLGFRN 385
             M  +  VW+EIV + +L  T++ED+ANW+  D         +    K    GF     
Sbjct: 316 REMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYR 375

Query: 386 SKNSFISWIDKVKAFKIVP 394
           + +S + WID ++  K++P
Sbjct: 376 TLDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of CmoCh01G018570.1 vs. NCBI nr
Match: gi|748038959|gb|AJE29749.1| (putative progesterone 5-beta-reductase [Cucurbita pepo])

HSP 1 Score: 818.5 bits (2113), Expect = 4.9e-234
Identity = 386/393 (98.22%), Postives = 388/393 (98.73%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of CmoCh01G018570.1 vs. NCBI nr
Match: gi|659093104|ref|XP_008447374.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo])

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-209
Identity = 337/393 (85.75%), Postives = 364/393 (92.62%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN   EI+NCE N 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE++I+  +QRH+PPFTED+PRL+ PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE +   SDADL+AEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEGERL L +LMK K+ VW+EIVKENQLQPTKLE++ NWW+ D+  S SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of CmoCh01G018570.1 vs. NCBI nr
Match: gi|449468584|ref|XP_004152001.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus])

HSP 1 Score: 726.1 bits (1873), Expect = 3.3e-206
Identity = 338/394 (85.79%), Postives = 360/394 (91.37%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN  TEIENCE N 
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE+II+  +QRHDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLL+EEIE+SQKKDLTWAVIRPN IFGFSPFSLMN+VGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE + V SDADLIAEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGF-EEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
           IE+YGF EEGE L L +LMK K+ VW+EIVKENQLQ TKLE++  WW  D   S SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           SMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]

BLAST of CmoCh01G018570.1 vs. NCBI nr
Match: gi|965662190|dbj|BAT77899.1| (hypothetical protein VIGAN_02051100 [Vigna angularis var. angularis])

HSP 1 Score: 671.0 bits (1730), Expect = 1.3e-189
Identity = 308/393 (78.37%), Postives = 343/393 (87.28%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEED+ PRSFESV LVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDTPRSFESVGLVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEY+QCDVSDP DAE+KLS LTDVT++F+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPPWNADHPIEYVQCDVSDPADAESKLSVLTDVTNIFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLRNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG+LC YAAICKHEG PL+
Sbjct: 181 YYTQEDILFEETAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSLCVYAAICKHEGIPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGT+ AWEGYS  SDADLIAEQHIW AVDPYA+NEAFNCSNGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTRGAWEGYSFSSDADLIAEQHIWAAVDPYARNEAFNCSNGDIFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E MK K  VW+EIV +NQL PTKL++V +WW  D+  S  G +DS
Sbjct: 301 IEEYGFEEGSGLRLSEAMKDKGPVWDEIVSQNQLLPTKLDEVGDWWFVDVIFSGEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNSFISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSFISWIDKSKGYKIVP 389

[Close]

BLAST of CmoCh01G018570.1 vs. NCBI nr
Match: gi|593269120|ref|XP_007136737.1| (hypothetical protein PHAVU_009G070100g [Phaseolus vulgaris])

HSP 1 Score: 670.6 bits (1729), Expect = 1.6e-189
Identity = 311/393 (79.13%), Postives = 341/393 (86.77%), Query Frame = 1


Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESV LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WNADHPIEY+QCDVSDP DAE+KLS LTDVTHVF+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG++C YAAICKHEG PL+
Sbjct: 181 YYNQEDILFEESAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSVCVYAAICKHEGVPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWEGYS  SDADLIAEQHIW AVDP A+NEAFNCSNGDVFKWKH WKVLAEQF 
Sbjct: 241 FPGTKGAWEGYSFSSDADLIAEQHIWAAVDPNARNEAFNCSNGDVFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E+MK K  VW+EIV  NQL PTKLE+V +WW  DI  SA G +DS
Sbjct: 301 IEEYGFEEGSHLKLSEVMKDKGPVWDEIVSANQLLPTKLEEVGDWWFVDIIFSAEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNS ISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSLISWIDKCKGYKIVP 389

The following BLAST results are available for this feature:

BLAST of CmoCh01G018570.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
VEP1_ARATH	2.2e-186	77.10	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 S...	[more]
5BPOR_DIGLA	7.2e-169	70.23	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1	[more]
IRIS_CATRO	3.6e-136	57.93	Iridoid synthase OS=Catharanthus roseus PE=1 SV=1	[more]
YJ72_SCHPO	2.2e-29	26.60	Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of CmoCh01G018570.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0B5CYJ8_CUCPE	3.4e-234	98.22	Putative progesterone 5-beta-reductase OS=Cucurbita pepo PE=2 SV=1	[more]
A0A0A0L8Y0_CUCSA	2.3e-206	85.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G610790 PE=4 SV=1	[more]
A0A0S3RB21_PHAAN	8.8e-190	78.37	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G051100 PE=...	[more]
V7AWY5_PHAVU	1.1e-189	79.13	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G070100g PE=4 SV=1	[more]
W9SGC8_9ROSA	4.8e-188	78.12	Uncharacterized protein OS=Morus notabilis GN=L484_011348 PE=4 SV=1	[more]

BLAST of CmoCh01G018570.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G24220.1	1.3e-187	77.10	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G58750.1	1.6e-62	37.47	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of CmoCh01G018570.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|748038959\|gb\|AJE29749.1\|	4.9e-234	98.22	putative progesterone 5-beta-reductase [Cucurbita pepo]	[more]
gi\|659093104\|ref\|XP_008447374.1\|	1.4e-209	85.75	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo]	[more]
gi\|449468584\|ref\|XP_004152001.1\|	3.3e-206	85.79	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus]	[more]
gi\|965662190\|dbj\|BAT77899.1\|	1.3e-189	78.37	hypothetical protein VIGAN_02051100 [Vigna angularis var. angularis]	[more]
gi\|593269120\|ref\|XP_007136737.1\|	1.6e-189	79.13	hypothetical protein PHAVU_009G070100g [Phaseolus vulgaris]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001509	Epimerase_deHydtase
IPR016040	NAD(P)-bd_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0050662	coenzyme binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008209	androgen metabolic process
biological_process	GO:0006699	bile acid biosynthetic process
biological_process	GO:0008207	C21-steroid hormone metabolic process
biological_process	GO:0008210	estrogen metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0010051	xylem and phloem pattern formation
cellular_component	GO:0005829	cytosol
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050662	coenzyme binding
molecular_function	GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function	GO:0047568	3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function	GO:0047787	delta4-3-oxosteroid 5beta-reductase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh01G018570	CmoCh01G018570	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh01G018570.1	CmoCh01G018570.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G018570.1.three_prime_UTR.1	CmoCh01G018570.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G018570.1.CDS.2	CmoCh01G018570.1.CDS.2	CDS
CmoCh01G018570.1.CDS.1	CmoCh01G018570.1.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G018570.1.five_prime_UTR.1	CmoCh01G018570.1.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh01G018570.1.exon.2	CmoCh01G018570.1.exon.2	exon
CmoCh01G018570.1.exon.1	CmoCh01G018570.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase, N-terminal domain	PFAM	PF01370	Epimerase	coord: 29..256 score: 6.
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 29..311 score: 1.3
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 28..302 score: 1.02
None	No IPR available	PANTHER	PTHR32487	FAMILY NOT NAMED	coord: 2..393 score: 1.3E
None	No IPR available	PANTHER	PTHR32487:SF0	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 2..393 score: 1.3E