CmoCh01G018570 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh01G018570
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Description(3-oxo-delta(4,5)-steroid 5-beta-reductase-like protein) (Progesterone 5-beta-reductase)
LocationCmo_Chr01 : 13503775 .. 13505613 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAGGAAGTAATGACCTTCCATAAACATTCGTTTCTGATATTGGTAGATGGTTACATAGGAAAATCCAGTCGGGAGTCCAGCGGTTCGGGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCACCTTATAGCCCTTTCAACACACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCCCTCCTCCCTAAATCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGGTAATTAAAACCCCTAACTCCCTATCTATGCCCCTGTTTCTATTACGTTTCTCTAATCAGCATCTCCGTTCCCAATTTTGCAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGATTGCCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTATGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTGGTAATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATAAGTAATAATCTTTAGAACGTTGTTACATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCT

mRNA sequence

AAAAAAAAAAAAAAAAAAAAGGAAGTAATGACCTTCCATAAACATTCGTTTCTGATATTGGTAGATGGTTACATAGGAAAATCCAGTCGGGAGTCCAGCGGTTCGGGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCACCTTATAGCCCTTTCAACACACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCCCTCCTCCCTAAATCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGATTGCCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTATGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTGGTAATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATAAGTAATAATCTTTAGAACGTTGTTACATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCT

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATCCAATGCGATGTCTCCGATCCGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATCATCCGACGCAGCGGCACGATCCGCCTTTCACGGAGGACATGCCTCGATTGGACTCCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACGTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGGGAGAGTTGATGAAGGGGAAAAATGCAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTTTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAAATCGTGCCCTGA
Homology
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BLAST of CmoCh01G018570 vs. Swiss-Prot
Match: VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 2.2e-186
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N+ D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV-DGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIET--VTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of CmoCh01G018570 vs. Swiss-Prot
Match: 5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 7.2e-169
Identity = 276/393 (70.23%), Postives = 317/393 (80.66%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARR RP W+ D+PI Y+QCD+SDP+D++AKLS LTDVTHVFYVTW N  TE ENCE N 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FE+      + HDPP+TED+PRL   NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY  ED++ EE+EK  K+ LTW+V RP  IFGFSP+S+MN+VGTLC YAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVEK--KEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           F G K+AW+GYS  SDADLIAE HIW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +    +EEG  L L +LMKGK  VWEEIV+EN L PTKL+DV  WW  D+ L     +DS
Sbjct: 301 VGCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

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BLAST of CmoCh01G018570 vs. Swiss-Prot
Match: IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 3.6e-136
Identity = 230/397 (57.93%), Postives = 291/397 (73.30%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQP---RSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWK 60
           MSWWW  +IGA K    ++++    +S++SVALV+GVTGIVG+SLAE+L L DTPGGPWK
Sbjct: 1   MSWWWKRSIGAGKNLPNQNKENGVCKSYKSVALVVGVTGIVGSSLAEVLKLPDTPGGPWK 60

Query: 61  VYGVARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCE 120
           VYGVARRP P W A  P+EYIQCDVSD ++  +KLS L D+TH+FYV+W       E+C+
Sbjct: 61  VYGVARRPCPVWLAKKPVEYIQCDVSDNQETISKLSPLKDITHIFYVSWIGS----EDCQ 120

Query: 121 VNGKMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDS 180
            N  M +N+L SVIPNA NL+H+CLQTG KHY G FE         HD PFTED+PRL+ 
Sbjct: 121 TNATMFKNILNSVIPNASNLQHVCLQTGIKHYFGIFEE--GSKVVPHDSPFTEDLPRLNV 180

Query: 181 PNFYYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGR 240
           PNFY+  ED+L+EE   + K +LTW+V RP  +FGFSP S+MNIV TLC YA ICKHE +
Sbjct: 181 PNFYHDLEDILYEE---TGKNNLTWSVHRPALVFGFSPCSMMNIVSTLCVYATICKHENK 240

Query: 241 PLKFPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAE 300
            L +PG+K++W  Y+   DADL+AE  IW AVDP AKN+  NC+NGDVFKWKH WK LAE
Sbjct: 241 ALVYPGSKNSWNCYADAVDADLVAEHEIWAAVDPKAKNQVLNCNNGDVFKWKHIWKKLAE 300

Query: 301 QFNIEDYGFEEG-ERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASG 360
           +F IE  G+ EG E++ L ELMK K+ VW+EIVK+N L PTKL+++A +W  DIA  +  
Sbjct: 301 EFGIEMVGYVEGKEQVSLAELMKDKDQVWDEIVKKNNLVPTKLKEIAAFWFADIAFCSEN 360

Query: 361 NIDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
            I SMNKSKE GFLGFRNS  SF+S IDK++ ++ +P
Sbjct: 361 LISSMNKSKELGFLGFRNSMKSFVSCIDKMRDYRFIP 388

[Close]
BLAST of CmoCh01G018570 vs. Swiss-Prot
Match: YJ72_SCHPO (Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC757.02c PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.2e-29
Identity = 108/406 (26.60%), Postives = 174/406 (42.86%), Query Frame = 1 

Query: 28  VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDPED 87
           VA+V G TG+ G ++ + L   D       +    +   P     H I+ +     +P+D
Sbjct: 7   VAIVTGATGLNGAAIIKRLSEDDNCKTIHCISRSLKDEYPRKIKHHSIDLLN---EEPKD 66

Query: 88  AEAKLSK--LTDVTHVFYVTWTNLPTEIENCEVNGKMLRNVLRSV-IPNAPNLRHICLQT 147
              K S   +  + + ++  +     E + CEVNG MLRN ++++ + +   LR + L T
Sbjct: 67  IAKKFSLEGVKGINYAYFAAYKEENNEEKLCEVNGNMLRNFVQALELTSIQTLRRVILTT 126

Query: 148 GTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNFYYTQEDLLFEEIEKSQKKDLTWAV 207
           G K Y G     +  P    D    E      +PNFYY QED+L +E    +K D T A+
Sbjct: 127 GLKFY-GLHLGEVRLPMIETDIRVPETFS--GTPNFYYVQEDIL-KEFSNGKKWDYTIAM 186

Query: 208 IRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAWEGYSVVSDADLIAEQH 267
             PN I G S  S MN   T+  YA +C+    P +FPG +  + G+  +S + LIA+  
Sbjct: 187 --PNDICGVSKGSYMNEAFTIALYALVCRELHEPFRFPGNEKFYLGFDDISYSKLIADFQ 246

Query: 268 IWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFNIE--------------DYGFEEGE 327
           +W         E FN  NGD+  W   W  +AE F +E              +       
Sbjct: 247 LWMTFKAECSEEKFNIVNGDIHSWSRTWPKIAEYFGVEVPKNQFATDFTLSTEVTLSTPS 306

Query: 328 RLPLGELMKG-KNAVWEEIVKENQLQ----PTKLED-----------------VANWWHT 387
            + L E   G K+    +I+ +  LQ      K++D                 V  W   
Sbjct: 307 PINLYEKELGIKHTPNSKIINQISLQQWVKQKKVQDAWRTIAEREKLNAHALEVGTWAFC 366

Query: 388 DIALSASGN-IDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           D     + N I SM+K+++ G+  + ++ + F    D++K  K +P
Sbjct: 367 DFLFGRTYNVISSMSKARKLGYTDYYDTFDGFKETFDELKKQKQIP 403

[Close]
BLAST of CmoCh01G018570 vs. TrEMBL
Match: A0A0B5CYJ8_CUCPE (Putative progesterone 5-beta-reductase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 3.4e-234
Identity = 386/393 (98.22%), Postives = 388/393 (98.73%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of CmoCh01G018570 vs. TrEMBL
Match: A0A0A0L8Y0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G610790 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-206
Identity = 338/394 (85.79%), Postives = 360/394 (91.37%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN  TEIENCE N 
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE+II+  +QRHDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLL+EEIE+SQKKDLTWAVIRPN IFGFSPFSLMN+VGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE + V SDADLIAEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGF-EEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
           IE+YGF EEGE L L +LMK K+ VW+EIVKENQLQ TKLE++  WW  D   S SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           SMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]
BLAST of CmoCh01G018570 vs. TrEMBL
Match: A0A0S3RB21_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G051100 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 8.8e-190
Identity = 308/393 (78.37%), Postives = 343/393 (87.28%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEED+ PRSFESV LVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDTPRSFESVGLVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEY+QCDVSDP DAE+KLS LTDVT++F+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPPWNADHPIEYVQCDVSDPADAESKLSVLTDVTNIFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLRNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG+LC YAAICKHEG PL+
Sbjct: 181 YYTQEDILFEETAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSLCVYAAICKHEGIPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGT+ AWEGYS  SDADLIAEQHIW AVDPYA+NEAFNCSNGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTRGAWEGYSFSSDADLIAEQHIWAAVDPYARNEAFNCSNGDIFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E MK K  VW+EIV +NQL PTKL++V +WW  D+  S  G +DS
Sbjct: 301 IEEYGFEEGSGLRLSEAMKDKGPVWDEIVSQNQLLPTKLDEVGDWWFVDVIFSGEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNSFISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSFISWIDKSKGYKIVP 389

[Close]
BLAST of CmoCh01G018570 vs. TrEMBL
Match: V7AWY5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G070100g PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.1e-189
Identity = 311/393 (79.13%), Postives = 341/393 (86.77%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESV LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WNADHPIEY+QCDVSDP DAE+KLS LTDVTHVF+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG++C YAAICKHEG PL+
Sbjct: 181 YYNQEDILFEESAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSVCVYAAICKHEGVPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWEGYS  SDADLIAEQHIW AVDP A+NEAFNCSNGDVFKWKH WKVLAEQF 
Sbjct: 241 FPGTKGAWEGYSFSSDADLIAEQHIWAAVDPNARNEAFNCSNGDVFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E+MK K  VW+EIV  NQL PTKLE+V +WW  DI  SA G +DS
Sbjct: 301 IEEYGFEEGSHLKLSEVMKDKGPVWDEIVSANQLLPTKLEEVGDWWFVDIIFSAEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNS ISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSLISWIDKCKGYKIVP 389

[Close]
BLAST of CmoCh01G018570 vs. TrEMBL
Match: W9SGC8_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_011348 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 4.8e-188
Identity = 307/393 (78.12%), Postives = 338/393 (86.01%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE+E P S++SV LV+GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEENEAPISYQSVGLVVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           V RRPRPNWNADHPIEYIQCD+SDPED   KLS LTDVTH+FYVTW N  TE+ENCEVNG
Sbjct: 61  VGRRPRPNWNADHPIEYIQCDISDPEDTNTKLSTLTDVTHIFYVTWANRATEVENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            M  NVLR++IPNAPNL HICLQTGTKHYIG FE+      + HDPPFTED+PRL+ PNF
Sbjct: 121 AMFHNVLRALIPNAPNLSHICLQTGTKHYIGPFESF--GKIKPHDPPFTEDLPRLEYPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYT ED+L EE+ K  K+ LTW+V RP+TIFGFSP+SLMN++GTLC YAAICKHEGRPLK
Sbjct: 181 YYTLEDVLQEEVRK--KESLTWSVHRPDTIFGFSPYSLMNLIGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK+AW+ YSV SDADLIAEQ IW AVD YAKNEAFNC+NGDVF+WKHFWKVLAEQF 
Sbjct: 241 FPGTKAAWDSYSVASDADLIAEQQIWAAVDLYAKNEAFNCNNGDVFRWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IED+G EEGERL L ELMK K  VWEEIVKENQL+PTKLE+V  WW  D+  S    + S
Sbjct: 301 IEDWGLEEGERLRLTELMKDKGPVWEEIVKENQLEPTKLEEVGVWWFADLMFSGRAPLSS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAYKIVP 389

[Close]
BLAST of CmoCh01G018570 vs. TAIR10
Match: AT4G24220.1 (AT4G24220.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 652.9 bits (1683), Expect = 1.3e-187
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N+ D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV-DGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIET--VTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of CmoCh01G018570 vs. TAIR10
Match: AT5G58750.1 (AT5G58750.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 237.3 bits (604), Expect = 1.6e-62
Identity = 142/379 (37.47%), Postives = 207/379 (54.62%), Query Frame = 1 

Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDP 85
           E+VAL+ GVTG+VG  + + L +S  PG  W++YGVAR P  N +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSK-PG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  EDAEAKLSKLTD-VTHVFYVTWTN-LPTEIENCEVNGK-MLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+HVF+VTW+   P + + C V  K ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDS-PNFYYTQEDLLFEEIEKSQKKDLT 205
           QTG KHY+   E  +          ++E+ PR  S  NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195

Query: 206 WAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAW-EGYSVVSDADLI 265
           W+V RP  + G S  +L N +G+LC Y A+CK+   P  F GT+  W E Y   SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVDPYA--KNEAFNCSNGDVFKWKHFWKVLAEQFNI---EDYGFEEGERLPLG 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  +   E   F+EG     G
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEG--FWFG 315

Query: 326 ELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGN-IDSMNKSKEHGFLGFRN 385
             M  +  VW+EIV + +L  T++ED+ANW+  D         +    K    GF     
Sbjct: 316 REMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYR 375

Query: 386 SKNSFISWIDKVKAFKIVP 394
           + +S + WID ++  K++P
Sbjct: 376 TLDSVLYWIDVMRDEKLIP 385

[Close]
BLAST of CmoCh01G018570 vs. NCBI nr
Match: gi|748038959|gb|AJE29749.1| (putative progesterone 5-beta-reductase [Cucurbita pepo])

HSP 1 Score: 818.5 bits (2113), Expect = 4.9e-234
Identity = 386/393 (98.22%), Postives = 388/393 (98.73%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of CmoCh01G018570 vs. NCBI nr
Match: gi|659093104|ref|XP_008447374.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo])

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-209
Identity = 337/393 (85.75%), Postives = 364/393 (92.62%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN   EI+NCE N 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE++I+  +QRH+PPFTED+PRL+ PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE +   SDADL+AEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEGERL L +LMK K+ VW+EIVKENQLQPTKLE++ NWW+ D+  S SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of CmoCh01G018570 vs. NCBI nr
Match: gi|449468584|ref|XP_004152001.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus])

HSP 1 Score: 726.1 bits (1873), Expect = 3.3e-206
Identity = 338/394 (85.79%), Postives = 360/394 (91.37%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSDP+DAE KLS+L DVTH+FYVTWTN  TEIENCE N 
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE+II+  +QRHDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLL+EEIE+SQKKDLTWAVIRPN IFGFSPFSLMN+VGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE + V SDADLIAEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGF-EEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
           IE+YGF EEGE L L +LMK K+ VW+EIVKENQLQ TKLE++  WW  D   S SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           SMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]
BLAST of CmoCh01G018570 vs. NCBI nr
Match: gi|965662190|dbj|BAT77899.1| (hypothetical protein VIGAN_02051100 [Vigna angularis var. angularis])

HSP 1 Score: 671.0 bits (1730), Expect = 1.3e-189
Identity = 308/393 (78.37%), Postives = 343/393 (87.28%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEED+ PRSFESV LVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDTPRSFESVGLVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEY+QCDVSDP DAE+KLS LTDVT++F+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPPWNADHPIEYVQCDVSDPADAESKLSVLTDVTNIFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            MLRNVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLRNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG+LC YAAICKHEG PL+
Sbjct: 181 YYTQEDILFEETAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSLCVYAAICKHEGIPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGT+ AWEGYS  SDADLIAEQHIW AVDPYA+NEAFNCSNGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTRGAWEGYSFSSDADLIAEQHIWAAVDPYARNEAFNCSNGDIFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E MK K  VW+EIV +NQL PTKL++V +WW  D+  S  G +DS
Sbjct: 301 IEEYGFEEGSGLRLSEAMKDKGPVWDEIVSQNQLLPTKLDEVGDWWFVDVIFSGEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNSFISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSFISWIDKSKGYKIVP 389

[Close]
BLAST of CmoCh01G018570 vs. NCBI nr
Match: gi|593269120|ref|XP_007136737.1| (hypothetical protein PHAVU_009G070100g [Phaseolus vulgaris])

HSP 1 Score: 670.6 bits (1729), Expect = 1.6e-189
Identity = 311/393 (79.13%), Postives = 341/393 (86.77%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESV LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WNADHPIEY+QCDVSDP DAE+KLS LTDVTHVF+V+WTN PTE ENCEVNG
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFE+I     Q H+PPFTED+PRLD+PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QED+LFEE  K  K+ LTW+V RP TIFGFSP+SLMN+VG++C YAAICKHEG PL+
Sbjct: 181 YYNQEDILFEESAK--KEGLTWSVHRPGTIFGFSPYSLMNLVGSVCVYAAICKHEGVPLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWEGYS  SDADLIAEQHIW AVDP A+NEAFNCSNGDVFKWKH WKVLAEQF 
Sbjct: 241 FPGTKGAWEGYSFSSDADLIAEQHIWAAVDPNARNEAFNCSNGDVFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG  L L E+MK K  VW+EIV  NQL PTKLE+V +WW  DI  SA G +DS
Sbjct: 301 IEEYGFEEGSHLKLSEVMKDKGPVWDEIVSANQLLPTKLEEVGDWWFVDIIFSAEGVLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNK+KEHGFLGFRNSKNS ISWIDK K +KIVP
Sbjct: 361 MNKAKEHGFLGFRNSKNSLISWIDKCKGYKIVP 389

The following BLAST results are available for this feature:
BLAST of CmoCh01G018570 vs. Swiss-Prot
Match NameE-valueIdentityDescription
VEP1_ARATH2.2e-18677.103-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 S... [more]
5BPOR_DIGLA7.2e-16970.233-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1[more]
IRIS_CATRO3.6e-13657.93Iridoid synthase OS=Catharanthus roseus PE=1 SV=1[more]
YJ72_SCHPO2.2e-2926.60Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
BLAST of CmoCh01G018570 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0B5CYJ8_CUCPE3.4e-23498.22Putative progesterone 5-beta-reductase OS=Cucurbita pepo PE=2 SV=1[more]
A0A0A0L8Y0_CUCSA2.3e-20685.79Uncharacterized protein OS=Cucumis sativus GN=Csa_3G610790 PE=4 SV=1[more]
A0A0S3RB21_PHAAN8.8e-19078.37Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.02G051100 PE=... [more]
V7AWY5_PHAVU1.1e-18979.13Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G070100g PE=4 SV=1[more]
W9SGC8_9ROSA4.8e-18878.12Uncharacterized protein OS=Morus notabilis GN=L484_011348 PE=4 SV=1[more]
BLAST of CmoCh01G018570 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G24220.11.3e-18777.10 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G58750.11.6e-6237.47 NAD(P)-binding Rossmann-fold superfamily protein[more]
BLAST of CmoCh01G018570 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|748038959|gb|AJE29749.1|4.9e-23498.22putative progesterone 5-beta-reductase [Cucurbita pepo][more]
gi|659093104|ref|XP_008447374.1|1.4e-20985.75PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo][more]
gi|449468584|ref|XP_004152001.1|3.3e-20685.79PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus][more]
gi|965662190|dbj|BAT77899.1|1.3e-18978.37hypothetical protein VIGAN_02051100 [Vigna angularis var. angularis][more]
gi|593269120|ref|XP_007136737.1|1.6e-18979.13hypothetical protein PHAVU_009G070100g [Phaseolus vulgaris][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001509Epimerase_deHydtase
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0050662coenzyme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008209 androgen metabolic process
biological_process GO:0006699 bile acid biosynthetic process
biological_process GO:0008207 C21-steroid hormone metabolic process
biological_process GO:0008210 estrogen metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0010051 xylem and phloem pattern formation
biological_process GO:0008150 biological_process
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0047568 3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function GO:0050662 coenzyme binding
molecular_function GO:0047787 delta4-3-oxosteroid 5beta-reductase activity
molecular_function GO:0035671 enone reductase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh01G018570.1CmoCh01G018570.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratase, N-terminal domainPFAMPF01370Epimerasecoord: 29..256
score: 6.
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 29..311
score: 1.3
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 28..302
score: 1.02
NoneNo IPR availablePANTHERPTHR32487FAMILY NOT NAMEDcoord: 2..393
score: 1.3E
NoneNo IPR availablePANTHERPTHR32487:SF03-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 2..393
score: 1.3E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh01G018570CmaCh09G002740Cucurbita maxima (Rimu)cmacmoB047
CmoCh01G018570CmaCh01G017980Cucurbita maxima (Rimu)cmacmoB469
CmoCh01G018570Cla020616Watermelon (97103) v1cmowmB434
CmoCh01G018570ClCG05G020410Watermelon (Charleston Gray)cmowcgB412
CmoCh01G018570Lsi04G015010Bottle gourd (USVL1VR-Ls)cmolsiB412
CmoCh01G018570Cp4.1LG06g01930Cucurbita pepo (Zucchini)cmocpeB465
CmoCh01G018570Cp4.1LG02g06610Cucurbita pepo (Zucchini)cmocpeB450
CmoCh01G018570Cla97C05G102150Watermelon (97103) v2cmowmbB461
CmoCh01G018570Bhi09G002679Wax gourdcmowgoB0598
CmoCh01G018570Carg21791Silver-seed gourdcarcmoB0441
CmoCh01G018570Carg10633Silver-seed gourdcarcmoB1044
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmoCh01G018570CmoCh09G002700Cucurbita moschata (Rifu)cmocmoB017
The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh01G018570Cucumber (Chinese Long) v3cmocucB0510
CmoCh01G018570Cucumber (Chinese Long) v3cmocucB0551
CmoCh01G018570Watermelon (97103) v2cmowmbB486
CmoCh01G018570Wax gourdcmowgoB0570
CmoCh01G018570Cucurbita moschata (Rifu)cmocmoB105
CmoCh01G018570Cucurbita moschata (Rifu)cmocmoB141
CmoCh01G018570Cucurbita moschata (Rifu)cmocmoB360
CmoCh01G018570Cucurbita moschata (Rifu)cmocmoB369
CmoCh01G018570Cucurbita moschata (Rifu)cmocmoB376
CmoCh01G018570Cucumber (Gy14) v1cgycmoB0626
CmoCh01G018570Cucumber (Gy14) v1cgycmoB0967
CmoCh01G018570Cucumber (Gy14) v1cgycmoB1090
CmoCh01G018570Cucurbita maxima (Rimu)cmacmoB184
CmoCh01G018570Cucurbita maxima (Rimu)cmacmoB779
CmoCh01G018570Wild cucumber (PI 183967)cmocpiB428
CmoCh01G018570Wild cucumber (PI 183967)cmocpiB465
CmoCh01G018570Wild cucumber (PI 183967)cmocpiB491
CmoCh01G018570Cucumber (Chinese Long) v2cmocuB424
CmoCh01G018570Cucumber (Chinese Long) v2cmocuB461
CmoCh01G018570Melon (DHL92) v3.5.1cmomeB398
CmoCh01G018570Melon (DHL92) v3.5.1cmomeB403
CmoCh01G018570Melon (DHL92) v3.5.1cmomeB422
CmoCh01G018570Watermelon (Charleston Gray)cmowcgB435
CmoCh01G018570Watermelon (97103) v1cmowmB416
CmoCh01G018570Cucurbita pepo (Zucchini)cmocpeB420
CmoCh01G018570Cucurbita pepo (Zucchini)cmocpeB440
CmoCh01G018570Cucurbita pepo (Zucchini)cmocpeB476
CmoCh01G018570Bottle gourd (USVL1VR-Ls)cmolsiB411
CmoCh01G018570Bottle gourd (USVL1VR-Ls)cmolsiB442
CmoCh01G018570Cucumber (Gy14) v2cgybcmoB193
CmoCh01G018570Cucumber (Gy14) v2cgybcmoB485
CmoCh01G018570Melon (DHL92) v3.6.1cmomedB461
CmoCh01G018570Melon (DHL92) v3.6.1cmomedB486
CmoCh01G018570Silver-seed gourdcarcmoB0259
CmoCh01G018570Silver-seed gourdcarcmoB0548