CmaCh20G007340.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh20G007340.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	RING/U-box superfamily protein
Location	Cma_Chr20 : 3425689 .. 3426372 (-)
Sequence length	684

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTCTTCCGCTACTTCTTCGCCGATTCAGCACGATCTTCACAGCGACGGCGACGAGCAATCGCCTCCTACTCTGGAAGTTCAAATGCGTTCTTACATACGTCTGATTTCAGGCGATTTAAACCAAATCGTCCTCGAAACTGCTCCGATTCCTCTGCGGAACAGTCTGTTTCCTCTGCCTCCGAACGCACGGAATATCGATCCGGTGTTCTTACAGATCTACATTGCGGAGATACTTTTATCGCTCAACATCGGCGCCGTAGCTTCCGAAGTCATCGCTGCACAGATCGCGTCGTTCACTCTCCAACTGGCGGAAGAACACAATGATACGAACGTTCACGTCGTCGCGGTGGTGGATAACGTCGTGGAGTCCAGGTTTTGGAGGTTGCAAGCGGTACCGGTGATGCAGGGCGGGAGAGCGGCGGAGGAGAGGAGGGAGGTTGTGGAGGAAGTAGCGGCGGCGAATGGGAGGTATAGAATGGAGAAAGTGGAGGAGAAGGAGGAGGAGGAAGTGGATTTAGGAAATTGCAGTATCTGTTTGGATGAGATGAAGTGCGAAGGGAGCGAGGTGATAAGAACTCCGTGCGGACATGTGTATCACGAATCATGCATCTTCAGGTGGCTTGATAATTCCGATTCTTGCCCCTTGTGTCGAACCCAATTTCCATTGATGGAGGACTGA

mRNA sequence

ATGATGTCTTCCGCTACTTCTTCGCCGATTCAGCACGATCTTCACAGCGACGGCGACGAGCAATCGCCTCCTACTCTGGAAGTTCAAATGCGTTCTTACATACGTCTGATTTCAGGCGATTTAAACCAAATCGTCCTCGAAACTGCTCCGATTCCTCTGCGGAACAGTCTGTTTCCTCTGCCTCCGAACGCACGGAATATCGATCCGGTGTTCTTACAGATCTACATTGCGGAGATACTTTTATCGCTCAACATCGGCGCCGTAGCTTCCGAAGTCATCGCTGCACAGATCGCGTCGTTCACTCTCCAACTGGCGGAAGAACACAATGATACGAACGTTCACGTCGTCGCGGTGGTGGATAACGTCGTGGAGTCCAGGTTTTGGAGGTTGCAAGCGGTACCGGTGATGCAGGGCGGGAGAGCGGCGGAGGAGAGGAGGGAGGTTGTGGAGGAAGTAGCGGCGGCGAATGGGAGGTATAGAATGGAGAAAGTGGAGGAGAAGGAGGAGGAGGAAGTGGATTTAGGAAATTGCAGTATCTGTTTGGATGAGATGAAGTGCGAAGGGAGCGAGGTGATAAGAACTCCGTGCGGACATGTGTATCACGAATCATGCATCTTCAGGTGGCTTGATAATTCCGATTCTTGCCCCTTGTGTCGAACCCAATTTCCATTGATGGAGGACTGA

Coding sequence (CDS)

ATGATGTCTTCCGCTACTTCTTCGCCGATTCAGCACGATCTTCACAGCGACGGCGACGAGCAATCGCCTCCTACTCTGGAAGTTCAAATGCGTTCTTACATACGTCTGATTTCAGGCGATTTAAACCAAATCGTCCTCGAAACTGCTCCGATTCCTCTGCGGAACAGTCTGTTTCCTCTGCCTCCGAACGCACGGAATATCGATCCGGTGTTCTTACAGATCTACATTGCGGAGATACTTTTATCGCTCAACATCGGCGCCGTAGCTTCCGAAGTCATCGCTGCACAGATCGCGTCGTTCACTCTCCAACTGGCGGAAGAACACAATGATACGAACGTTCACGTCGTCGCGGTGGTGGATAACGTCGTGGAGTCCAGGTTTTGGAGGTTGCAAGCGGTACCGGTGATGCAGGGCGGGAGAGCGGCGGAGGAGAGGAGGGAGGTTGTGGAGGAAGTAGCGGCGGCGAATGGGAGGTATAGAATGGAGAAAGTGGAGGAGAAGGAGGAGGAGGAAGTGGATTTAGGAAATTGCAGTATCTGTTTGGATGAGATGAAGTGCGAAGGGAGCGAGGTGATAAGAACTCCGTGCGGACATGTGTATCACGAATCATGCATCTTCAGGTGGCTTGATAATTCCGATTCTTGCCCCTTGTGTCGAACCCAATTTCCATTGATGGAGGACTGA

Protein sequence

MMSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLISGDLNQIVLETAPIPLRNSLFPLPPNARNIDPVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHVVAVVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED

Homology

[Close]

BLAST of CmaCh20G007340.1 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.4e-09
Identity = 22/57 (38.60%), Postives = 40/57 (70.18%), Query Frame = 1


Query: 167 KEEEEVDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFP 224
           K+  + ++  C++C+DE + +GS+V + PC HV+H+ C+  WL+  +SCP+CR + P
Sbjct: 206 KDMLKSEMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of CmaCh20G007340.1 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.3e-08
Identity = 23/48 (47.92%), Postives = 30/48 (62.50%), Query Frame = 1


Query: 176 NCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFP 224
           NC +C DE +  GSE  + PC H+YH  CI  WL   +SCP+CR + P
Sbjct: 189 NCPVCKDEFEL-GSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of CmaCh20G007340.1 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.3e-08
Identity = 20/51 (39.22%), Postives = 35/51 (68.63%), Query Frame = 1


Query: 173 DLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFP 224
           +   C++C+D+ + EG+E  + PC H+YH+ C+  WL+  +SCP+CR + P
Sbjct: 220 EFNQCAVCMDDFE-EGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELP 269

[Close]

BLAST of CmaCh20G007340.1 vs. Swiss-Prot
Match: RN181_MOUSE (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 4.8e-08
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 1


Query: 177 CSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED 228
           C +CL E + E + VI  PC H++H +CI  WL  ++SCPLCR + P  +D
Sbjct: 88  CPVCLLEFEAEET-VIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDDD 137

[Close]

BLAST of CmaCh20G007340.1 vs. Swiss-Prot
Match: RN181_RAT (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.3e-08
Identity = 23/51 (45.10%), Postives = 32/51 (62.75%), Query Frame = 1


Query: 177 CSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED 228
           C +CL E + E   VI  PC H++H +CI  WL  ++SCPLCR + P  +D
Sbjct: 88  CPVCLLEFE-EEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDDD 137

[Close]

BLAST of CmaCh20G007340.1 vs. TrEMBL
Match: A0A0A0K696_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.0e-32
Identity = 101/236 (42.80%), Postives = 144/236 (61.02%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLIS-----GDLNQIVLETAPIPLRNS 61
           MS A S+PIQ+ L++ G   +   L+V +    ++IS     G  ++++ +T  IP  N+
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60

Query: 62  LFPLPPNARNID-PVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHV 121
           LF L  + R +  P+F   YI +IL SLNI   AS+ +A +IAS  LQ+ + +++TN H+
Sbjct: 61  LFYL--SLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHI 120

Query: 122 VAVVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGR----YRMEKVEEKEEEE 181
           +A VD      F RL  +P M GG A  E   V++E   A  R     R E++  +E+ E
Sbjct: 121 LAEVD------FIRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKME 180

Query: 182 VDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED 228
            +LG+CSICLDE+ CE  EV+R PCGHVYHESCIF+WL+N +SCPLCR   PL  D
Sbjct: 181 -ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHHD 221

[Close]

BLAST of CmaCh20G007340.1 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.0e-32
Identity = 101/236 (42.80%), Postives = 144/236 (61.02%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLIS-----GDLNQIVLETAPIPLRNS 61
           MS A S+PIQ+ L++ G   +   L+V +    ++IS     G  ++++ +T  IP  N+
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60

Query: 62  LFPLPPNARNID-PVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHV 121
           LF L  + R +  P+F   YI +IL SLNI   AS+ +A +IAS  LQ+ + +++TN H+
Sbjct: 61  LFYL--SLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHI 120

Query: 122 VAVVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGR----YRMEKVEEKEEEE 181
           +A VD      F RL  +P M GG A  E   V++E   A  R     R E++  +E+ E
Sbjct: 121 LAEVD------FIRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKME 180

Query: 182 VDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED 228
            +LG+CSICLDE+ CE  EV+R PCGHVYHESCIF+WL+N +SCPLCR   PL  D
Sbjct: 181 -ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHHD 221

[Close]

BLAST of CmaCh20G007340.1 vs. TrEMBL
Match: A0A0A0KYC0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G003650 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 7.2e-27
Identity = 94/227 (41.41%), Postives = 128/227 (56.39%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLISGDL--NQIVLETAPIPLRNSLFP 61
           MS   SSPI + L+++G       LEVQ+    R IS      +I+ ET P P  N+LF 
Sbjct: 1   MSFEISSPIPYLLNANGFGNG--VLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFY 60

Query: 62  LPPNARNIDPVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHN--DTNVHVVA 121
           L  +     P+F   +I + L SLNI     E IA QIASF   +   +   ++N +V+A
Sbjct: 61  LSVHQLQ-HPLF---HIRQFLYSLNI---RRETIARQIASFVAHMCNGNTGWNSNFYVIA 120

Query: 122 VVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGNC 181
            VD V   R  R++  P  +G R        VE         R+ K++ +EE+    G+C
Sbjct: 121 RVDLV---RVIRIEEQPRAEGWRGV-----AVE---------RLSKLKSEEEK----GDC 180

Query: 182 SICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPL 225
           S+CLDE+ CE  EVIR PCGHVYHESCIF+WL++S+SCPLCR+ FPL
Sbjct: 181 SVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPL 197

[Close]

BLAST of CmaCh20G007340.1 vs. TrEMBL
Match: A0A0A0LW68_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573640 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.1e-19
Identity = 74/199 (37.19%), Postives = 106/199 (53.27%), Query Frame = 1


Query: 26  LEVQMRSYIRLISGDLNQIVLETAPIPLR--NSLFPLPPNARNI--DPVFLQIYIAEILL 85
           LE+Q R    L+     QI+ +T PI L   N+ FPL  N  +   +P     Y+A++  
Sbjct: 27  LEIQRRISHSLMFNFPPQIIHQTLPILLDYDNNFFPLHFNFFHSYGEPSSFTFYVAQLFS 86

Query: 86  SLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHVVAVVDNVVESRFWRLQAVPVMQGGRA 145
           SLNI ++ASE +A  I     Q+A E+++ + H+  ++D +  +    L          +
Sbjct: 87  SLNINSIASEAMARNIFLIANQVANENSNASFHIAVIIDFINFTWMCPL----------S 146

Query: 146 AEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGNCSICLDEMKCEGSEVI-RTPCGHVY 205
              R+    EV+          VE+K+    DLG+CSICLDE+     EV+ +  CGHVY
Sbjct: 147 FSTRQLTGNEVSM---------VEQKKIGN-DLGDCSICLDELSPSNREVVLKISCGHVY 205

Query: 206 HESCIFRWLDNSDSCPLCR 220
           HE CI RWLDNSDSCPLCR
Sbjct: 207 HELCILRWLDNSDSCPLCR 205

[Close]

BLAST of CmaCh20G007340.1 vs. TrEMBL
Match: A0A0A0LHI3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-16
Identity = 70/230 (30.43%), Postives = 112/230 (48.70%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLISGDLNQIVLETAPIPLRNSLFPLP 61
           MSS T+  +   + S         +E+++R + +L+S   NQ + +T P  + N+LF  P
Sbjct: 1   MSSTTNLSLDCCVRSIPTTADKQLVEIRLRRFSKLVSPFSNQNIQQTFPTSISNALFHFP 60

Query: 62  -PNARNIDPVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHVVAVVD 121
            P     +P     ++ + L   +     S  I   I+SF LQLA  + + N H++A VD
Sbjct: 61  LPELELENPSLFHDFLLQFLSPAD-----SAAIFHNISSFALQLAAGNLNLNFHLIATVD 120

Query: 122 NV----VESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGN 181
            +    +       Q  P ++ G  A     V+E       R   EK ++    E   G 
Sbjct: 121 LLYTISINLNLNPPQYSPPVRNGVPAS----VME-------RLMNEKYDDGNGGEGMGGQ 180

Query: 182 CSICLDEMKCEGSE----VIRTPCGHVYHESCIFRWLDNSDSCPLCRTQF 223
           C +C +++ CE  E      R PCGHVYH+SCI +WL+ ++SCPLCR++F
Sbjct: 181 CCVCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSEF 214

[Close]

BLAST of CmaCh20G007340.1 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 62.4 bits (150), Expect = 4.2e-10
Identity = 22/57 (38.60%), Postives = 40/57 (70.18%), Query Frame = 1


Query: 167 KEEEEVDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFP 224
           K+  + ++  C++C+DE + +GS+V + PC HV+H+ C+  WL+  +SCP+CR + P
Sbjct: 206 KDMLKSEMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of CmaCh20G007340.1 vs. TAIR10
Match: AT2G40830.2 (AT2G40830.2 RING-H2 finger C1A)

HSP 1 Score: 61.6 bits (148), Expect = 7.1e-10
Identity = 23/48 (47.92%), Postives = 30/48 (62.50%), Query Frame = 1


Query: 176 NCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFP 224
           NC +C DE +  GSE  + PC H+YH  CI  WL   +SCP+CR + P
Sbjct: 189 NCPVCKDEFEL-GSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of CmaCh20G007340.1 vs. TAIR10
Match: AT1G60360.1 (AT1G60360.1 RING/U-box superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 1.2e-09
Identity = 21/53 (39.62%), Postives = 33/53 (62.26%), Query Frame = 1


Query: 173 DLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLM 226
           D+  C++C++E    G +    PC H+YH+ CI  WL  ++SCP+CR   PL+
Sbjct: 220 DMSQCTVCMEEF-IVGGDATELPCKHIYHKDCIVPWLRLNNSCPICRRDLPLV 271

[Close]

BLAST of CmaCh20G007340.1 vs. TAIR10
Match: AT3G02340.1 (AT3G02340.1 RING/U-box superfamily protein)

HSP 1 Score: 60.5 bits (145), Expect = 1.6e-09
Identity = 29/62 (46.77%), Postives = 37/62 (59.68%), Query Frame = 1


Query: 164 VEEKEEEEVDLGN--CSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQ 223
           V E   EE+D GN  C++C DEM  E  +V R PC H YH  CI  WL   ++CP+CR +
Sbjct: 320 VVELAVEELDKGNNVCAVCKDEMLVE-EKVRRLPCSHFYHGECIIPWLGIRNTCPVCRYE 379

[Close]

BLAST of CmaCh20G007340.1 vs. TAIR10
Match: AT1G18770.1 (AT1G18770.1 RING/U-box superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 3.5e-09
Identity = 21/47 (44.68%), Postives = 30/47 (63.83%), Query Frame = 1


Query: 177 CSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFP 224
           C ICL+E   EG  V+  PCGH + + C+ +W + + SCPLCR + P
Sbjct: 59  CIICLEEFS-EGRRVVTLPCGHDFDDECVLKWFETNHSCPLCRFKLP 104

[Close]

BLAST of CmaCh20G007340.1 vs. NCBI nr
Match: gi|449454422|ref|XP_004144954.1| (PREDICTED: uncharacterized protein LOC101214496 [Cucumis sativus])

HSP 1 Score: 147.5 bits (371), Expect = 2.8e-32
Identity = 101/236 (42.80%), Postives = 144/236 (61.02%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLIS-----GDLNQIVLETAPIPLRNS 61
           MS A S+PIQ+ L++ G   +   L+V +    ++IS     G  ++++ +T  IP  N+
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60

Query: 62  LFPLPPNARNID-PVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHV 121
           LF L  + R +  P+F   YI +IL SLNI   AS+ +A +IAS  LQ+ + +++TN H+
Sbjct: 61  LFYL--SLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHI 120

Query: 122 VAVVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGR----YRMEKVEEKEEEE 181
           +A VD      F RL  +P M GG A  E   V++E   A  R     R E++  +E+ E
Sbjct: 121 LAEVD------FIRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKME 180

Query: 182 VDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED 228
            +LG+CSICLDE+ CE  EV+R PCGHVYHESCIF+WL+N +SCPLCR   PL  D
Sbjct: 181 -ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHHD 221

[Close]

BLAST of CmaCh20G007340.1 vs. NCBI nr
Match: gi|778730084|ref|XP_011659694.1| (PREDICTED: uncharacterized protein LOC105436225 [Cucumis sativus])

HSP 1 Score: 147.5 bits (371), Expect = 2.8e-32
Identity = 101/236 (42.80%), Postives = 144/236 (61.02%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLIS-----GDLNQIVLETAPIPLRNS 61
           MS A S+PIQ+ L++ G   +   L+V +    ++IS     G  ++++ +T  IP  N+
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNA 60

Query: 62  LFPLPPNARNID-PVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHV 121
           LF L  + R +  P+F   YI +IL SLNI   AS+ +A +IAS  LQ+ + +++TN H+
Sbjct: 61  LFYL--SLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNFHI 120

Query: 122 VAVVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGR----YRMEKVEEKEEEE 181
           +A VD      F RL  +P M GG A  E   V++E   A  R     R E++  +E+ E
Sbjct: 121 LAEVD------FIRLIWLPSMGGGGAGAEETVVLDEAPPAVKRGVGVARGERLRSEEKME 180

Query: 182 VDLGNCSICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPLMED 228
            +LG+CSICLDE+ CE  EV+R PCGHVYHESCIF+WL+N +SCPLCR   PL  D
Sbjct: 181 -ELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHHD 221

[Close]

BLAST of CmaCh20G007340.1 vs. NCBI nr
Match: gi|778697402|ref|XP_011654314.1| (PREDICTED: E3 ubiquitin-protein ligase RNF115-like [Cucumis sativus])

HSP 1 Score: 129.0 bits (323), Expect = 1.0e-26
Identity = 94/227 (41.41%), Postives = 128/227 (56.39%), Query Frame = 1


Query: 2   MSSATSSPIQHDLHSDGDEQSPPTLEVQMRSYIRLISGDL--NQIVLETAPIPLRNSLFP 61
           MS   SSPI + L+++G       LEVQ+    R IS      +I+ ET P P  N+LF 
Sbjct: 1   MSFEISSPIPYLLNANGFGNG--VLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFY 60

Query: 62  LPPNARNIDPVFLQIYIAEILLSLNIGAVASEVIAAQIASFTLQLAEEHN--DTNVHVVA 121
           L  +     P+F   +I + L SLNI     E IA QIASF   +   +   ++N +V+A
Sbjct: 61  LSVHQLQ-HPLF---HIRQFLYSLNI---RRETIARQIASFVAHMCNGNTGWNSNFYVIA 120

Query: 122 VVDNVVESRFWRLQAVPVMQGGRAAEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGNC 181
            VD V   R  R++  P  +G R        VE         R+ K++ +EE+    G+C
Sbjct: 121 RVDLV---RVIRIEEQPRAEGWRGV-----AVE---------RLSKLKSEEEK----GDC 180

Query: 182 SICLDEMKCEGSEVIRTPCGHVYHESCIFRWLDNSDSCPLCRTQFPL 225
           S+CLDE+ CE  EVIR PCGHVYHESCIF+WL++S+SCPLCR+ FPL
Sbjct: 181 SVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPL 197

[Close]

BLAST of CmaCh20G007340.1 vs. NCBI nr
Match: gi|659100547|ref|XP_008451147.1| (PREDICTED: E3 ubiquitin-protein ligase ATL59-like [Cucumis melo])

HSP 1 Score: 109.4 bits (272), Expect = 8.4e-21
Identity = 73/208 (35.10%), Postives = 113/208 (54.33%), Query Frame = 1


Query: 26  LEVQMRSYIRLISGDLNQIVLETAPIPLRN--SLFPLPPNARN--IDPVFLQIYIAEILL 85
           LE+Q R    L+    +Q + +T+ I + N  + FPLP N  +   +P     Y+ ++L 
Sbjct: 28  LEIQRRFSHSLMFDFPHQTIHQTSSISIDNYDNYFPLPLNFFHNYYEPSSFPSYVTQLLS 87

Query: 86  SLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHVVAVVDNVVESRFWRLQAVPVMQGGRA 145
           SL+I +V SEVIA +I      +A+E+++ + H+V  VD +   +F  + ++ ++     
Sbjct: 88  SLDINSVNSEVIAREIVLIANHVAKENSNASFHIVVTVDFI---QFTWMHSLSLLTQQST 147

Query: 146 AEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGNCSICLDEMKCEGSEV-IRTPCGHVY 205
             E R V        G   +EK         DL +CSICL+E  C   ++ ++ PCGHVY
Sbjct: 148 RNEVRMV--------GWKEIEK---------DLDDCSICLEEFSCNNRKIGLKIPCGHVY 207

Query: 206 HESCIFRWLDNSDSCPLCRTQ-FPLMED 228
           HESCI +WL+NS SCPLCR   FP  E+
Sbjct: 208 HESCILKWLENSHSCPLCRRLIFPRFEN 215

[Close]

BLAST of CmaCh20G007340.1 vs. NCBI nr
Match: gi|700211049|gb|KGN66145.1| (hypothetical protein Csa_1G573640 [Cucumis sativus])

HSP 1 Score: 105.1 bits (261), Expect = 1.6e-19
Identity = 74/199 (37.19%), Postives = 106/199 (53.27%), Query Frame = 1


Query: 26  LEVQMRSYIRLISGDLNQIVLETAPIPLR--NSLFPLPPNARNI--DPVFLQIYIAEILL 85
           LE+Q R    L+     QI+ +T PI L   N+ FPL  N  +   +P     Y+A++  
Sbjct: 27  LEIQRRISHSLMFNFPPQIIHQTLPILLDYDNNFFPLHFNFFHSYGEPSSFTFYVAQLFS 86

Query: 86  SLNIGAVASEVIAAQIASFTLQLAEEHNDTNVHVVAVVDNVVESRFWRLQAVPVMQGGRA 145
           SLNI ++ASE +A  I     Q+A E+++ + H+  ++D +  +    L          +
Sbjct: 87  SLNINSIASEAMARNIFLIANQVANENSNASFHIAVIIDFINFTWMCPL----------S 146

Query: 146 AEERREVVEEVAAANGRYRMEKVEEKEEEEVDLGNCSICLDEMKCEGSEVI-RTPCGHVY 205
              R+    EV+          VE+K+    DLG+CSICLDE+     EV+ +  CGHVY
Sbjct: 147 FSTRQLTGNEVSM---------VEQKKIGN-DLGDCSICLDELSPSNREVVLKISCGHVY 205

Query: 206 HESCIFRWLDNSDSCPLCR 220
           HE CI RWLDNSDSCPLCR
Sbjct: 207 HELCILRWLDNSDSCPLCR 205

The following BLAST results are available for this feature:

BLAST of CmaCh20G007340.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RNG1L_ARATH	7.4e-09	38.60	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]
RHC1A_ARATH	1.3e-08	47.92	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2...	[more]
RING1_GOSHI	1.3e-08	39.22	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
RN181_MOUSE	4.8e-08	45.10	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus GN=Rnf181 PE=1 SV=1	[more]
RN181_RAT	6.3e-08	45.10	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus GN=Rnf181 PE=1 SV=1	[more]

BLAST of CmaCh20G007340.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K696_CUCSA	2.0e-32	42.80	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1	[more]
A0A0A0K2W2_CUCSA	2.0e-32	42.80	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1	[more]
A0A0A0KYC0_CUCSA	7.2e-27	41.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G003650 PE=4 SV=1	[more]
A0A0A0LW68_CUCSA	1.1e-19	37.19	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573640 PE=4 SV=1	[more]
A0A0A0LHI3_CUCSA	1.1e-16	30.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1	[more]

BLAST of CmaCh20G007340.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G19950.1	4.2e-10	38.60	RING/U-box superfamily protein	[more]
AT2G40830.2	7.1e-10	47.92	RING-H2 finger C1A	[more]
AT1G60360.1	1.2e-09	39.62	RING/U-box superfamily protein	[more]
AT3G02340.1	1.6e-09	46.77	RING/U-box superfamily protein	[more]
AT1G18770.1	3.5e-09	44.68	RING/U-box superfamily protein	[more]

BLAST of CmaCh20G007340.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449454422\|ref\|XP_004144954.1\|	2.8e-32	42.80	PREDICTED: uncharacterized protein LOC101214496 [Cucumis sativus]	[more]
gi\|778730084\|ref\|XP_011659694.1\|	2.8e-32	42.80	PREDICTED: uncharacterized protein LOC105436225 [Cucumis sativus]	[more]
gi\|778697402\|ref\|XP_011654314.1\|	1.0e-26	41.41	PREDICTED: E3 ubiquitin-protein ligase RNF115-like [Cucumis sativus]	[more]
gi\|659100547\|ref\|XP_008451147.1\|	8.4e-21	35.10	PREDICTED: E3 ubiquitin-protein ligase ATL59-like [Cucumis melo]	[more]
gi\|700211049\|gb\|KGN66145.1\|	1.6e-19	37.19	hypothetical protein Csa_1G573640 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh20G007340	CmaCh20G007340	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh20G007340.1	CmaCh20G007340.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G007340.1.CDS.1	CmaCh20G007340.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G007340.1.exon.1	CmaCh20G007340.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..219 score: 7.5
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..218 score: 1.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 177..219 score: 13
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 176..220 score: 3.6
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 102..223 score: 1.7E-16coord: 1..42 score: 1.7
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 161..224 score: 5.82