CmaCh20G000560.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh20G000560.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Delta-12-fatty acid desaturase
Location	Cma_Chr20 : 248698 .. 249152 (-)
Sequence length	267

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCTGTCCCGAATAAAAACTGAAGCCCACCAAAGGGAAAAATGGAAGATCAAAATGGTGGTATCTCGCAGTTTCTTTTTCTTTTTCTTTGCTCTTCTTCCTTTTATGGTCTGTCTGTACGTATTTGAAGAAAGACCCGTAGTGTTCTACTGGTGATGAAGGATGAACCTTTTTAATCCCTTAATTAAGGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

mRNA sequence

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

Coding sequence (CDS)

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

Protein sequence

MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFFFFSPLKESSGTATSSESIFIGGFTIYISYVVANSIHLIN

Homology

[Close]

BLAST of CmaCh20G000560.1 vs. Swiss-Prot
Match: FAD12_TRIKI (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.1e-13
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1


Query: 7  MEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          MEK V ERVPHAKPPFTLSQIKK +PPHCFHRSLLR+FS
Sbjct: 1  MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFS 39

[Close]

BLAST of CmaCh20G000560.1 vs. Swiss-Prot
Match: FAD2_VERFO (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.5e-10
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560.1 vs. Swiss-Prot
Match: FAD2_CALOF (Delta(12) fatty acid desaturase FAD2 OS=Calendula officinalis GN=FAD2 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.9e-10
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = 1


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFF 50
          G+K E    +RVPH KPPFT+  IKKAIPPHCF+RS++R+FS  F+
Sbjct: 14 GNKTEPEPIQRVPHEKPPFTVGDIKKAIPPHCFNRSVIRSFSYVFY 59

[Close]

BLAST of CmaCh20G000560.1 vs. Swiss-Prot
Match: FAD12_PUNGR (Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-09
Identity = 29/44 (65.91%), Postives = 35/44 (79.55%), Query Frame = 1


Query: 2  GVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          G G +  +   +RVP AKPPFTLS+IKKAIPPHCF RSLL++FS
Sbjct: 15 GEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFS 58

[Close]

BLAST of CmaCh20G000560.1 vs. Swiss-Prot
Match: FADX_TRIKI (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAC PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.9e-09
Identity = 30/47 (63.83%), Postives = 35/47 (74.47%), Query Frame = 1


Query: 5  SKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFFFF 52
          S  + A  ER+ HAKPPF++SQIKKAIPPHCF RSL R+FS   F F
Sbjct: 14 SSTKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFDF 60

[Close]

BLAST of CmaCh20G000560.1 vs. TrEMBL
Match: A0A088MFF4_CANSA (Delta 12 desaturase OS=Cannabis sativa GN=FAD2A PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.1e-10
Identity = 35/53 (66.04%), Postives = 41/53 (77.36%), Query Frame = 1


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFF--FFFSPL 55
          GSK    + ERVPH KPPFTLS+IKKAIPPHCF RSL+R+F+C F   FF+ L
Sbjct: 16 GSKNNNRLIERVPHTKPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASL 68

[Close]

BLAST of CmaCh20G000560.1 vs. TrEMBL
Match: A0A059DA68_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03641 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-08
Identity = 31/45 (68.89%), Postives = 35/45 (77.78%), Query Frame = 1


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          M V SK ++    RVPHAKPPFTL +IKKAIPPHCF RSL R+FS
Sbjct: 7  MSVPSKTDEGGLRRVPHAKPPFTLGEIKKAIPPHCFQRSLARSFS 51

[Close]

BLAST of CmaCh20G000560.1 vs. TrEMBL
Match: A1YZD3_9ROSI (Delta-12 oleic acid desaturase OS=Vernicia montana GN=FAD2 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560.1 vs. TrEMBL
Match: I6LVA4_VERFO (Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560.1 vs. TrEMBL
Match: I6LVA3_VERFO (Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560.1 vs. TAIR10
Match: AT3G12120.1 (AT3G12120.1 fatty acid desaturase 2)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-07
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K E    +RVP  KPPF++  +KKAIPPHCF RS+ R+FS
Sbjct: 15 KSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFS 54

[Close]

BLAST of CmaCh20G000560.1 vs. NCBI nr
Match: gi|75328134|sp|Q84UB9.1|FAD12_TRIKI (RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2)

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-11
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1


Query: 7  MEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          MEK V ERVPHAKPPFTLSQIKK +PPHCFHRSLLR+FS
Sbjct: 1  MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFS 39

[Close]

BLAST of CmaCh20G000560.1 vs. NCBI nr
Match: gi|682235498|gb|AIN36675.1| (delta 12 desaturase [Cannabis sativa])

HSP 1 Score: 72.4 bits (176), Expect = 4.4e-10
Identity = 35/53 (66.04%), Postives = 41/53 (77.36%), Query Frame = 1


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFF--FFFSPL 55
          GSK    + ERVPH KPPFTLS+IKKAIPPHCF RSL+R+F+C F   FF+ L
Sbjct: 16 GSKNNNRLIERVPHTKPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASL 68

[Close]

BLAST of CmaCh20G000560.1 vs. NCBI nr
Match: gi|702278873|ref|XP_010044976.1| (PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [Eucalyptus grandis])

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-08
Identity = 31/45 (68.89%), Postives = 35/45 (77.78%), Query Frame = 1


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          M V SK ++    RVPHAKPPFTL +IKKAIPPHCF RSL R+FS
Sbjct: 7  MSVPSKTDEGGLRRVPHAKPPFTLGEIKKAIPPHCFQRSLARSFS 51

[Close]

BLAST of CmaCh20G000560.1 vs. NCBI nr
Match: gi|332384315|gb|AEE69020.1| (Fad2 [Vernicia fordii])

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560.1 vs. NCBI nr
Match: gi|119655554|gb|ABL86147.1| (delta-12 oleic acid desaturase [Vernicia montana])

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

The following BLAST results are available for this feature:

BLAST of CmaCh20G000560.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FAD12_TRIKI	1.1e-13	87.18	Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1	[more]
FAD2_VERFO	1.5e-10	72.50	Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1	[more]
FAD2_CALOF	9.9e-10	60.87	Delta(12) fatty acid desaturase FAD2 OS=Calendula officinalis GN=FAD2 PE=2 SV=1	[more]
FAD12_PUNGR	1.7e-09	65.91	Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2	[more]
FADX_TRIKI	2.9e-09	63.83	Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes k...	[more]

BLAST of CmaCh20G000560.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A088MFF4_CANSA	3.1e-10	66.04	Delta 12 desaturase OS=Cannabis sativa GN=FAD2A PE=2 SV=1	[more]
A0A059DA68_EUCGR	1.3e-08	68.89	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03641 PE=4 SV=1	[more]
A1YZD3_9ROSI	1.7e-08	72.50	Delta-12 oleic acid desaturase OS=Vernicia montana GN=FAD2 PE=2 SV=1	[more]
I6LVA4_VERFO	1.7e-08	72.50	Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1	[more]
I6LVA3_VERFO	1.7e-08	72.50	Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1	[more]

BLAST of CmaCh20G000560.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G12120.1	3.7e-07	57.50	fatty acid desaturase 2	[more]

BLAST of CmaCh20G000560.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|75328134\|sp\|Q84UB9.1\|FAD12_TRIKI	1.8e-11	87.18	RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2	[more]
gi\|682235498\|gb\|AIN36675.1\|	4.4e-10	66.04	delta 12 desaturase [Cannabis sativa]	[more]
gi\|702278873\|ref\|XP_010044976.1\|	1.9e-08	68.89	PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [...	[more]
gi\|332384315\|gb\|AEE69020.1\|	2.4e-08	72.50	Fad2 [Vernicia fordii]	[more]
gi\|119655554\|gb\|ABL86147.1\|	2.4e-08	72.50	delta-12 oleic acid desaturase [Vernicia montana]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016720	delta12-fatty acid dehydrogenase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh20G000560	CmaCh20G000560	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh20G000560.1	CmaCh20G000560.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G000560.1.CDS.2	CmaCh20G000560.1.CDS.2	CDS
CmaCh20G000560.1.CDS.1	CmaCh20G000560.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G000560.1.exon.2	CmaCh20G000560.1.exon.2	exon
CmaCh20G000560.1.exon.1	CmaCh20G000560.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR32100	FAMILY NOT NAMED	coord: 6..45 score: 4.2
None	No IPR available	PANTHER	PTHR32100:SF13	OMEGA-6 FATTY ACID DESATURASE, ENDOPLASMIC RETICULUM	coord: 6..45 score: 4.2