CmaCh20G000560 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh20G000560
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Delta-12-fatty acid desaturase
Location	Cma_Chr20 : 248698 .. 249152 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCTGTCCCGAATAAAAACTGAAGCCCACCAAAGGGAAAAATGGAAGATCAAAATGGTGGTATCTCGCAGTTTCTTTTTCTTTTTCTTTGCTCTTCTTCCTTTTATGGTCTGTCTGTACGTATTTGAAGAAAGACCCGTAGTGTTCTACTGGTGATGAAGGATGAACCTTTTTAATCCCTTAATTAAGGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

mRNA sequence

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

Coding sequence (CDS)

ATGGGAGTTGGAAGCAAAATGGAAAAGGCGGTCCATGAGCGAGTTCCACACGCAAAGCCTCCTTTCACTCTCAGCCAAATCAAGAAAGCCATTCCTCCCCACTGCTTCCACCGCTCTCTTCTCCGCACCTTCTCCTGCTTCTTTTTCTTTTTTTCCCCATTGAAGGAGTCGTCTGGTACCGCAACAAGCTCTGAGTCAATTTTTATTGGTGGGTTTACAATATATATCAGTTATGTTGTTGCTAATTCTATTCATTTGATTAACTAA

Protein sequence

MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFFFFSPLKESSGTATSSESIFIGGFTIYISYVVANSIHLIN

Homology

[Close]

BLAST of CmaCh20G000560 vs. Swiss-Prot
Match: FAD12_TRIKI (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.1e-13
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1


Query: 7  MEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          MEK V ERVPHAKPPFTLSQIKK +PPHCFHRSLLR+FS
Sbjct: 1  MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFS 39

[Close]

BLAST of CmaCh20G000560 vs. Swiss-Prot
Match: FAD2_VERFO (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.5e-10
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. Swiss-Prot
Match: FAD2_CALOF (Delta(12) fatty acid desaturase FAD2 OS=Calendula officinalis GN=FAD2 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 9.9e-10
Identity = 28/46 (60.87%), Postives = 36/46 (78.26%), Query Frame = 1


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFF 50
          G+K E    +RVPH KPPFT+  IKKAIPPHCF+RS++R+FS  F+
Sbjct: 14 GNKTEPEPIQRVPHEKPPFTVGDIKKAIPPHCFNRSVIRSFSYVFY 59

[Close]

BLAST of CmaCh20G000560 vs. Swiss-Prot
Match: FAD12_PUNGR (Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-09
Identity = 29/44 (65.91%), Postives = 35/44 (79.55%), Query Frame = 1


Query: 2  GVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          G G +  +   +RVP AKPPFTLS+IKKAIPPHCF RSLL++FS
Sbjct: 15 GEGDEKSQKPVQRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFS 58

[Close]

BLAST of CmaCh20G000560 vs. Swiss-Prot
Match: FADX_TRIKI (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAC PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.9e-09
Identity = 30/47 (63.83%), Postives = 35/47 (74.47%), Query Frame = 1


Query: 5  SKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFFFFF 52
          S  + A  ER+ HAKPPF++SQIKKAIPPHCF RSL R+FS   F F
Sbjct: 14 SSTKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFDF 60

[Close]

BLAST of CmaCh20G000560 vs. TrEMBL
Match: A0A088MFF4_CANSA (Delta 12 desaturase OS=Cannabis sativa GN=FAD2A PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.1e-10
Identity = 35/53 (66.04%), Postives = 41/53 (77.36%), Query Frame = 1


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFF--FFFSPL 55
          GSK    + ERVPH KPPFTLS+IKKAIPPHCF RSL+R+F+C F   FF+ L
Sbjct: 16 GSKNNNRLIERVPHTKPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASL 68

[Close]

BLAST of CmaCh20G000560 vs. TrEMBL
Match: A0A059DA68_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03641 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-08
Identity = 31/45 (68.89%), Postives = 35/45 (77.78%), Query Frame = 1


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          M V SK ++    RVPHAKPPFTL +IKKAIPPHCF RSL R+FS
Sbjct: 7  MSVPSKTDEGGLRRVPHAKPPFTLGEIKKAIPPHCFQRSLARSFS 51

[Close]

BLAST of CmaCh20G000560 vs. TrEMBL
Match: A1YZD3_9ROSI (Delta-12 oleic acid desaturase OS=Vernicia montana GN=FAD2 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. TrEMBL
Match: I6LVA4_VERFO (Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. TrEMBL
Match: I6LVA3_VERFO (Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. TAIR10
Match: AT3G12120.1 (AT3G12120.1 fatty acid desaturase 2)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-07
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K E    +RVP  KPPF++  +KKAIPPHCF RS+ R+FS
Sbjct: 15 KSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: gi|75328134|sp|Q84UB9.1|FAD12_TRIKI (RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2)

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-11
Identity = 34/39 (87.18%), Postives = 36/39 (92.31%), Query Frame = 1


Query: 7  MEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          MEK V ERVPHAKPPFTLSQIKK +PPHCFHRSLLR+FS
Sbjct: 1  MEKGVQERVPHAKPPFTLSQIKKVVPPHCFHRSLLRSFS 39

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: gi|682235498|gb|AIN36675.1| (delta 12 desaturase [Cannabis sativa])

HSP 1 Score: 72.4 bits (176), Expect = 4.4e-10
Identity = 35/53 (66.04%), Postives = 41/53 (77.36%), Query Frame = 1


Query: 4  GSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFSCFF--FFFSPL 55
          GSK    + ERVPH KPPFTLS+IKKAIPPHCF RSL+R+F+C F   FF+ L
Sbjct: 16 GSKNNNRLIERVPHTKPPFTLSEIKKAIPPHCFKRSLIRSFACVFHDLFFASL 68

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: gi|702278873|ref|XP_010044976.1| (PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [Eucalyptus grandis])

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-08
Identity = 31/45 (68.89%), Postives = 35/45 (77.78%), Query Frame = 1


Query: 1  MGVGSKMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          M V SK ++    RVPHAKPPFTL +IKKAIPPHCF RSL R+FS
Sbjct: 7  MSVPSKTDEGGLRRVPHAKPPFTLGEIKKAIPPHCFQRSLARSFS 51

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: gi|332384315|gb|AEE69020.1| (Fad2 [Vernicia fordii])

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

[Close]

BLAST of CmaCh20G000560 vs. NCBI nr
Match: gi|119655554|gb|ABL86147.1| (delta-12 oleic acid desaturase [Vernicia montana])

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-08
Identity = 29/40 (72.50%), Postives = 35/40 (87.50%), Query Frame = 1


Query: 6  KMEKAVHERVPHAKPPFTLSQIKKAIPPHCFHRSLLRTFS 46
          K+E  V +RVPH+KPPFTL Q+KKAIPPHCF RS+LR+FS
Sbjct: 15 KLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFS 54

The following BLAST results are available for this feature:

BLAST of CmaCh20G000560 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FAD12_TRIKI	1.1e-13	87.18	Delta(12)-oleate desaturase OS=Trichosanthes kirilowii GN=FAD2 PE=2 SV=1	[more]
FAD2_VERFO	1.5e-10	72.50	Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii GN=FAD2 PE=1 SV=1	[more]
FAD2_CALOF	9.9e-10	60.87	Delta(12) fatty acid desaturase FAD2 OS=Calendula officinalis GN=FAD2 PE=2 SV=1	[more]
FAD12_PUNGR	1.7e-09	65.91	Delta(12)-acyl-lipid-desaturase OS=Punica granatum GN=FAD12 PE=2 SV=2	[more]
FADX_TRIKI	2.9e-09	63.83	Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes k...	[more]

BLAST of CmaCh20G000560 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A088MFF4_CANSA	3.1e-10	66.04	Delta 12 desaturase OS=Cannabis sativa GN=FAD2A PE=2 SV=1	[more]
A0A059DA68_EUCGR	1.3e-08	68.89	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B03641 PE=4 SV=1	[more]
A1YZD3_9ROSI	1.7e-08	72.50	Delta-12 oleic acid desaturase OS=Vernicia montana GN=FAD2 PE=2 SV=1	[more]
I6LVA4_VERFO	1.7e-08	72.50	Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1	[more]
I6LVA3_VERFO	1.7e-08	72.50	Fad2 OS=Vernicia fordii GN=fad2 PE=4 SV=1	[more]

BLAST of CmaCh20G000560 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G12120.1	3.7e-07	57.50	fatty acid desaturase 2	[more]

BLAST of CmaCh20G000560 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|75328134\|sp\|Q84UB9.1\|FAD12_TRIKI	1.8e-11	87.18	RecName: Full=Delta(12)-oleate desaturase; Short=PgFAD2	[more]
gi\|682235498\|gb\|AIN36675.1\|	4.4e-10	66.04	delta 12 desaturase [Cannabis sativa]	[more]
gi\|702278873\|ref\|XP_010044976.1\|	1.9e-08	68.89	PREDICTED: omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2-like [...	[more]
gi\|332384315\|gb\|AEE69020.1\|	2.4e-08	72.50	Fad2 [Vernicia fordii]	[more]
gi\|119655554\|gb\|ABL86147.1\|	2.4e-08	72.50	delta-12 oleic acid desaturase [Vernicia montana]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016720	delta12-fatty acid dehydrogenase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh20G000560.1	CmaCh20G000560.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR32100	FAMILY NOT NAMED	coord: 6..45 score: 4.2
None	No IPR available	PANTHER	PTHR32100:SF13	OMEGA-6 FATTY ACID DESATURASE, ENDOPLASMIC RETICULUM	coord: 6..45 score: 4.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh20G000560	CmoCh02G012550	Cucurbita moschata (Rifu)	cmacmoB555
CmaCh20G000560	Cp4.1LG05g05130	Cucurbita pepo (Zucchini)	cmacpeB586
CmaCh20G000560	CsaV3_4G023720	Cucumber (Chinese Long) v3	cmacucB0660

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh20G000560	Cucurbita maxima (Rimu)	cmacmaB111
CmaCh20G000560	Cucurbita maxima (Rimu)	cmacmaB364
CmaCh20G000560	Cucurbita maxima (Rimu)	cmacmaB470
CmaCh20G000560	Cucurbita maxima (Rimu)	cmacmaB494
CmaCh20G000560	Cucumber (Gy14) v1	cgycmaB0051
CmaCh20G000560	Cucumber (Gy14) v1	cgycmaB0398
CmaCh20G000560	Cucumber (Gy14) v1	cgycmaB0423
CmaCh20G000560	Cucurbita moschata (Rifu)	cmacmoB538
CmaCh20G000560	Cucurbita moschata (Rifu)	cmacmoB550
CmaCh20G000560	Cucurbita moschata (Rifu)	cmacmoB572
CmaCh20G000560	Wild cucumber (PI 183967)	cmacpiB543
CmaCh20G000560	Wild cucumber (PI 183967)	cmacpiB554
CmaCh20G000560	Wild cucumber (PI 183967)	cmacpiB581
CmaCh20G000560	Cucumber (Chinese Long) v2	cmacuB549
CmaCh20G000560	Cucumber (Chinese Long) v2	cmacuB577
CmaCh20G000560	Melon (DHL92) v3.5.1	cmameB514
CmaCh20G000560	Melon (DHL92) v3.5.1	cmameB521
CmaCh20G000560	Watermelon (Charleston Gray)	cmawcgB489
CmaCh20G000560	Watermelon (97103) v1	cmawmB531
CmaCh20G000560	Cucurbita pepo (Zucchini)	cmacpeB564
CmaCh20G000560	Cucurbita pepo (Zucchini)	cmacpeB566
CmaCh20G000560	Cucurbita pepo (Zucchini)	cmacpeB567
CmaCh20G000560	Bottle gourd (USVL1VR-Ls)	cmalsiB500
CmaCh20G000560	Cucumber (Gy14) v2	cgybcmaB522
CmaCh20G000560	Melon (DHL92) v3.6.1	cmamedB591
CmaCh20G000560	Melon (DHL92) v3.6.1	cmamedB598
CmaCh20G000560	Silver-seed gourd	carcmaB0627
CmaCh20G000560	Silver-seed gourd	carcmaB0732
CmaCh20G000560	Silver-seed gourd	carcmaB1267
CmaCh20G000560	Cucumber (Chinese Long) v3	cmacucB0647
CmaCh20G000560	Cucumber (Chinese Long) v3	cmacucB0688
CmaCh20G000560	Watermelon (97103) v2	cmawmbB577
CmaCh20G000560	Wax gourd	cmawgoB0706
CmaCh20G000560	Wax gourd	cmawgoB0693