CmaCh19G006090.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh19G006090.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Calcium binding protein
Location	Cma_Chr19 : 6647034 .. 6648209 (+)
Sequence length	633

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAATGAACTCGAACCTCCTGGACCTGCAATACAGTGTCTTAAAGAAAAAGTTCCTCCGGGAACCCTCCCGTCTGTTCTCCTTCAAGAACCGACAGAGCTCAGGACTCCGGCAGGCGTTGCAGCCGAGCATGGAGGAGATAAAGCAGATCTTCAAATGGTTCGACACTAACAGAGATGGGAGGATCTCAAAGCATGAATACAGGGGAATTCTGGAGGCACTGGGGAGAGGGAGCTCCATGGAAGAGGTGCAGAAGATTTTCAAGGCAGTGGACACTGACGGCGATGGTTACATAAACTTGAACGAGTTTATGGAGGTTCTCCGGAGCGGAGTTCAGACTCGAGAGGTGGAATCTGCTTTCAAGACGTTTGATGTGAATGGGGATCAGAAGATTAGTGCCGAGGAAGTGATGAGAGTGTTGAAGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGGAACGGCGACGGCATGGTGGATATGGATGAGTTTATGACAATGATGACTCGGTCTGCAAAGTATGTTTAGAGGATGAGAGTTTCAACTTTTAAGGTTCTTTAATGGCGTTTGATTGTTAAAAAGTGTTAGTATATTGTAAAGGCTTTGTATAAATTCGACCCTACTATTATTTCCATCCCATAACAATTCCAACTTCTCTTTTAGTTAAACTGCTTCGGGTGAATCGAAACATGATAACAATGACATTTTTTATAAGCTTACAAACACTACTTTCACTCGTAAGTAAGTCTATATGTTTTGTTACCACTTTCTTCTACTGATATTTTAATAAACCAAGTCAAGGTTTAGAACTAGCTGGGATTCGAGTGATTCTTTCAACCTAATTGAAACAGAGGAGAAAAAAATTTCAAACCCAATAATTACCACTGGGACGTTTTCTTTTGCTAAAAGCTTACTAATCATCTATCAGGAGAAGAAAACTAGCACTTTCCAATTCAGTTCCAACAATAAACAGCAGGAAAAGAAGAAAAAAAAAAAACGATGATAAGACGAATGATTAAATATCAGGATAAGAAGAGCGTACATTATAAATCAATGGCTAGCTCGTCTCCAATCGGTACCTTGTTTCGTAGTTCGCATAAGGATATTGATGGTCTAACACTTTACATCGAGCTGCTATTGGGAAGATAA

mRNA sequence

ATGCCAATGAACTCGAACCTCCTGGACCTGCAATACAGTGTCTTAAAGAAAAAGTTCCTCCGGGAACCCTCCCGTCTGTTCTCCTTCAAGAACCGACAGAGCTCAGGACTCCGGCAGGCGTTGCAGCCGAGCATGGAGGAGATAAAGCAGATCTTCAAATGGTTCGACACTAACAGAGATGGGAGGATCTCAAAGCATGAATACAGGGGAATTCTGGAGGCACTGGGGAGAGGGAGCTCCATGGAAGAGGTGCAGAAGATTTTCAAGGCAGTGGACACTGACGGCGATGGTTACATAAACTTGAACGAGTTTATGGAGGTTCTCCGGAGCGGAGTTCAGACTCGAGAGGTGGAATCTGCTTTCAAGACGTTTGATGTGAATGGGGATCAGAAGATTAGTGCCGAGGAAGTGATGAGAGTGTTGAAGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGGAACGGCGACGGCATGGTGGATATGGATGAGTTTATGACAATGATGACTCGGTCTGCAAACTCGTCTCCAATCGGTACCTTGTTTCGTAGTTCGCATAAGGATATTGATGGTCTAACACTTTACATCGAGCTGCTATTGGGAAGATAA

Coding sequence (CDS)

ATGCCAATGAACTCGAACCTCCTGGACCTGCAATACAGTGTCTTAAAGAAAAAGTTCCTCCGGGAACCCTCCCGTCTGTTCTCCTTCAAGAACCGACAGAGCTCAGGACTCCGGCAGGCGTTGCAGCCGAGCATGGAGGAGATAAAGCAGATCTTCAAATGGTTCGACACTAACAGAGATGGGAGGATCTCAAAGCATGAATACAGGGGAATTCTGGAGGCACTGGGGAGAGGGAGCTCCATGGAAGAGGTGCAGAAGATTTTCAAGGCAGTGGACACTGACGGCGATGGTTACATAAACTTGAACGAGTTTATGGAGGTTCTCCGGAGCGGAGTTCAGACTCGAGAGGTGGAATCTGCTTTCAAGACGTTTGATGTGAATGGGGATCAGAAGATTAGTGCCGAGGAAGTGATGAGAGTGTTGAAGGGATTAGGGGAAAAGTGTAGCATTGAGGATTGCCGGCGGATGGTGAGGGCGGTGGACAGGAACGGCGACGGCATGGTGGATATGGATGAGTTTATGACAATGATGACTCGGTCTGCAAACTCGTCTCCAATCGGTACCTTGTTTCGTAGTTCGCATAAGGATATTGATGGTCTAACACTTTACATCGAGCTGCTATTGGGAAGATAA

Protein sequence

MPMNSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLRSGVQTREVESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRSANSSPIGTLFRSSHKDIDGLTLYIELLLGR

Homology

[Close]

BLAST of CmaCh19G006090.1 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.4e-46
Identity = 95/182 (52.20%), Postives = 138/182 (75.82%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQA-----LQPSMEEIKQIFKWFDTN 63
           N + L+LQY + K K LR+PSR+FS ++RQSSGL         QPS+ E++++F  FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTRE 123
           +DG+IS+ EY+ +L ALG+  ++E+V KIFKAVD DGDG+I+  EF++  +   G+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTM 179
           + ++F TFD+NGD KISAEEVM VL  LGE+CS+EDC RMVRAVD +GDG+V+M+EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of CmaCh19G006090.1 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-27
Identity = 59/138 (42.75%), Postives = 89/138 (64.49%), Query Frame = 1


Query: 45  MEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEF 104
           + E++ +FK FD N DG+IS  E   I+ +LG     EE++K    +D  GDGYIN  EF
Sbjct: 35  IRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEF 94

Query: 105 MEVLRSGVQTREV----ESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAV 164
           +E+   G+   +V    + AF  +D++G+  ISAEE+  VL+ LG++CSI +CR+M+  V
Sbjct: 95  VELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGV 154

Query: 165 DRNGDGMVDMDEFMTMMT 179
           D++GDG +D +EF  MMT
Sbjct: 155 DKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CmaCh19G006090.1 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-27
Identity = 61/140 (43.57%), Postives = 95/140 (67.86%), Query Frame = 1


Query: 44  SMEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNE 103
           SME+IK++F+ FD N DG+IS  E + ++ AL   +S EE + + K  D DG+G+I+L+E
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDE 71

Query: 104 FMEVLRSGVQT------REVESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMV 163
           F+ + +   Q+      R+++ AF  +D++ + +ISA E+  V+K LGEKCSI+DC+RM+
Sbjct: 72  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 131

Query: 164 RAVDRNGDGMVDMDEFMTMM 178
             VD +GDG VD +EF  MM
Sbjct: 132 NKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmaCh19G006090.1 vs. Swiss-Prot
Match: CML27_ARATH (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-27
Identity = 61/136 (44.85%), Postives = 88/136 (64.71%), Query Frame = 1


Query: 46  EEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFM 105
           EE+K++F  FD+N DG+IS  E  G+ +A+G   +  E+ ++ + VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVLRSGVQTREVESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGD 165
            + RS     E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD +GD
Sbjct: 82  TLCRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADGD 141

Query: 166 GMVDMDEFMTMMTRSA 182
           G V+ +EF  MMT S+
Sbjct: 142 GNVNFEEFQKMMTSSS 157

[Close]

BLAST of CmaCh19G006090.1 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 2.5e-27
Identity = 59/157 (37.58%), Postives = 103/157 (65.61%), Query Frame = 1


Query: 35  SGLRQALQPSMEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTD 94
           +G+ ++   SM++IK++F+ FD N DG+IS  E + ++ AL   +S EE   + K  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVLRSGV--------QTREVESAFKTFDVNGDQKISAEEVMRVLKGLGE 154
           G+G+I+L+EF+ + + G+           +++ AF+ +D++G+ +ISA+E+  V+K LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRSANS 184
           KCS++DC++M+  VD +GDG V+ DEF  MM+    +
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMSNGGGA 161

[Close]

BLAST of CmaCh19G006090.1 vs. TrEMBL
Match: A0A0A0K804_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 8.8e-80
Identity = 155/183 (84.70%), Postives = 171/183 (93.44%), Query Frame = 1


Query: 1   MPMNSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRD 60
           MP+N N L+LQY+V KKKFLREPSRLFSFK+RQSSGLR+ALQPS EEIKQIF  FDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLRS--GVQTREVE 120
           GRISKHEYRGIL+ALGRG+SMEEVQKIF+AVD+DGDGYINLNEFMEV RS  GVQ +EVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 SAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMT 180
            AFKTFD+NGD+KISAEEVMRVLKGLGEKCSIEDCRRMVRAVD +GDGMVD++EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSA 182
           RSA
Sbjct: 181 RSA 183

[Close]

BLAST of CmaCh19G006090.1 vs. TrEMBL
Match: M5VNS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 9.8e-55
Identity = 105/179 (58.66%), Postives = 146/179 (81.56%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRI 63
           ++N LD QY++ K+KFLR+PS +FSF++RQ+SG+  + +P+  E+K++F  FD+N+DG+I
Sbjct: 3   SANFLDFQYNLSKRKFLRKPSHVFSFRDRQNSGVSPSFEPNPNEMKRVFDKFDSNKDGKI 62

Query: 64  SKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTREVESAF 123
           S+ EY+ IL ALG+G+ + EV KIF+  D DGDG+IN  EFMEV +   G++T ++++AF
Sbjct: 63  SQPEYKAILRALGKGNMVGEVPKIFRIADLDGDGFINFKEFMEVHKKGGGIRTIDIQNAF 122

Query: 124 KTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRS 181
           +TFD+NGD KISAEEVM VL  LGE CS+EDC+RMVRAVD +GDG+VD+DEFMTMMTRS
Sbjct: 123 RTFDLNGDGKISAEEVMEVLGRLGESCSLEDCQRMVRAVDTDGDGVVDIDEFMTMMTRS 181

[Close]

BLAST of CmaCh19G006090.1 vs. TrEMBL
Match: B9HS04_POPTR (Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 2.2e-54
Identity = 106/182 (58.24%), Postives = 144/182 (79.12%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRI 63
           N + L+ QY + + KFLR+PSRLFS ++RQSSG+    QP+M+E++Q+F  FD+N+DG+I
Sbjct: 3   NLSFLEFQYKLSRNKFLRKPSRLFSSRDRQSSGMLSTFQPNMKEMRQVFDKFDSNKDGKI 62

Query: 64  SKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTREVESAF 123
           S+ EY+  L ALG+G+ + EV KIF+ VD DGDG+I+  EF+E  +   G++T ++++AF
Sbjct: 63  SQQEYKDTLRALGQGNMLGEVPKIFQVVDLDGDGFIDFKEFVEAQKKGGGIRTTDIQTAF 122

Query: 124 KTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRSA 183
           +TFD NGD KISAEEVM VL+ LGE+CS+EDCRRMV AVD +GDGMV+MDEFMTMMTRS 
Sbjct: 123 QTFDSNGDGKISAEEVMEVLRRLGERCSLEDCRRMVNAVDIDGDGMVNMDEFMTMMTRSM 182

[Close]

BLAST of CmaCh19G006090.1 vs. TrEMBL
Match: V4SBR8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029360mg PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-51
Identity = 102/179 (56.98%), Postives = 137/179 (76.54%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRI 63
           N + LD QY + K KFLR+PSRLFS ++RQ+S L    Q    E+K++F  FD+N+DG+I
Sbjct: 3   NLSFLDFQYKISKNKFLRKPSRLFSSRDRQNSSLLPTFQADRNEMKRVFDRFDSNKDGKI 62

Query: 64  SKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTREVESAF 123
           S+ EY+ IL A+G+ S + EV KIF+ VD DGDG+I+  EFME  +   G++T +++SAF
Sbjct: 63  SQMEYKAILRAIGQASMISEVPKIFQVVDLDGDGFIDFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 KTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRS 181
           +TFD N D KISAEE++ +L+ LGE CS+EDCR+MVRAVD +GDGMV+MDEFMTMMTRS
Sbjct: 123 RTFDKNDDGKISAEEILEMLRRLGESCSLEDCRKMVRAVDTDGDGMVNMDEFMTMMTRS 181

[Close]

BLAST of CmaCh19G006090.1 vs. TrEMBL
Match: A0A067F0V1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041237mg PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-51
Identity = 102/179 (56.98%), Postives = 137/179 (76.54%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRI 63
           N + LD QY + K KFLR+PSRLFS ++RQ+S L    Q    E+K++F  FD+N+DG+I
Sbjct: 3   NLSFLDFQYKISKNKFLRKPSRLFSSRDRQNSSLLPTFQADRNEMKRVFDRFDSNKDGKI 62

Query: 64  SKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTREVESAF 123
           S+ EY+ IL A+G+ S + EV KIF+ VD DGDG+I+  EFME  +   G++T +++SAF
Sbjct: 63  SQMEYKAILRAIGQASMISEVPKIFQVVDLDGDGFIDFKEFMEAHKKGGGIRTMDIQSAF 122

Query: 124 KTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRS 181
           +TFD N D KISAEE++ +L+ LGE CS+EDCR+MVRAVD +GDGMV+MDEFMTMMTRS
Sbjct: 123 RTFDKNDDGKISAEEILEMLRRLGESCSLEDCRKMVRAVDTDGDGMVNMDEFMTMMTRS 181

[Close]

BLAST of CmaCh19G006090.1 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 187.6 bits (475), Expect = 8.0e-48
Identity = 95/182 (52.20%), Postives = 138/182 (75.82%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQA-----LQPSMEEIKQIFKWFDTN 63
           N + L+LQY + K K LR+PSR+FS ++RQSSGL         QPS+ E++++F  FD +
Sbjct: 3   NVSFLELQYKLSKNKMLRKPSRMFS-RDRQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLD 62

Query: 64  RDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTRE 123
           +DG+IS+ EY+ +L ALG+  ++E+V KIFKAVD DGDG+I+  EF++  +   G+++ +
Sbjct: 63  KDGKISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSD 122

Query: 124 VESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTM 179
           + ++F TFD+NGD KISAEEVM VL  LGE+CS+EDC RMVRAVD +GDG+V+M+EF+ M
Sbjct: 123 IRNSFWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKM 182

[Close]

BLAST of CmaCh19G006090.1 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 124.8 bits (312), Expect = 6.3e-29
Identity = 59/138 (42.75%), Postives = 89/138 (64.49%), Query Frame = 1


Query: 45  MEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEF 104
           + E++ +FK FD N DG+IS  E   I+ +LG     EE++K    +D  GDGYIN  EF
Sbjct: 35  IRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEF 94

Query: 105 MEVLRSGVQTREV----ESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAV 164
           +E+   G+   +V    + AF  +D++G+  ISAEE+  VL+ LG++CSI +CR+M+  V
Sbjct: 95  VELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGV 154

Query: 165 DRNGDGMVDMDEFMTMMT 179
           D++GDG +D +EF  MMT
Sbjct: 155 DKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of CmaCh19G006090.1 vs. TAIR10
Match: AT1G18210.1 (AT1G18210.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 124.8 bits (312), Expect = 6.3e-29
Identity = 61/136 (44.85%), Postives = 88/136 (64.71%), Query Frame = 1


Query: 46  EEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFM 105
           EE+K++F  FD+N DG+IS  E  G+ +A+G   +  E+ ++ + VDTD DGYINL+EF 
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 106 EVLRSGVQTREVESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGD 165
            + RS     E+  AF  +D + +  ISA E+ +VL  LG  CS+EDC RM+  VD +GD
Sbjct: 82  TLCRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDADGD 141

Query: 166 GMVDMDEFMTMMTRSA 182
           G V+ +EF  MMT S+
Sbjct: 142 GNVNFEEFQKMMTSSS 157

[Close]

BLAST of CmaCh19G006090.1 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 124.8 bits (312), Expect = 6.3e-29
Identity = 61/140 (43.57%), Postives = 95/140 (67.86%), Query Frame = 1


Query: 44  SMEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNE 103
           SME+IK++F+ FD N DG+IS  E + ++ AL   +S EE + + K  D DG+G+I+L+E
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDE 71

Query: 104 FMEVLRSGVQT------REVESAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMV 163
           F+ + +   Q+      R+++ AF  +D++ + +ISA E+  V+K LGEKCSI+DC+RM+
Sbjct: 72  FVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMI 131

Query: 164 RAVDRNGDGMVDMDEFMTMM 178
             VD +GDG VD +EF  MM
Sbjct: 132 NKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmaCh19G006090.1 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 123.6 bits (309), Expect = 1.4e-28
Identity = 59/157 (37.58%), Postives = 103/157 (65.61%), Query Frame = 1


Query: 35  SGLRQALQPSMEEIKQIFKWFDTNRDGRISKHEYRGILEALGRGSSMEEVQKIFKAVDTD 94
           +G+ ++   SM++IK++F+ FD N DG+IS  E + ++ AL   +S EE   + K  D D
Sbjct: 5   NGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLD 64

Query: 95  GDGYINLNEFMEVLRSGV--------QTREVESAFKTFDVNGDQKISAEEVMRVLKGLGE 154
           G+G+I+L+EF+ + + G+           +++ AF+ +D++G+ +ISA+E+  V+K LGE
Sbjct: 65  GNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGE 124

Query: 155 KCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRSANS 184
           KCS++DC++M+  VD +GDG V+ DEF  MM+    +
Sbjct: 125 KCSVQDCKKMISKVDIDGDGCVNFDEFKKMMSNGGGA 161

[Close]

BLAST of CmaCh19G006090.1 vs. NCBI nr
Match: gi|659109907|ref|XP_008454944.1| (PREDICTED: calmodulin-like protein 1 [Cucumis melo])

HSP 1 Score: 308.5 bits (789), Expect = 8.8e-81
Identity = 157/183 (85.79%), Postives = 172/183 (93.99%), Query Frame = 1


Query: 1   MPMNSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRD 60
           MP+N N LDLQY+V KKKFLREPSRLFSFK+RQSSGLR+ALQPS+EEIKQIF  FDTN+D
Sbjct: 1   MPINPNFLDLQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSLEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLRS--GVQTREVE 120
           GRISKHEYRGIL+ALGRGSSMEEVQKIF+AVD+DGDGYINLNEFMEV RS  GVQ +EVE
Sbjct: 61  GRISKHEYRGILKALGRGSSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 SAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMT 180
            AFKTFD+NGD+KISAEEVMRVLKGLGEKCSIEDCRRMVRAVD +GDGMVD++EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSA 182
           RSA
Sbjct: 181 RSA 183

[Close]

BLAST of CmaCh19G006090.1 vs. NCBI nr
Match: gi|778725125|ref|XP_004137114.2| (PREDICTED: calmodulin-like protein 1 [Cucumis sativus])

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-79
Identity = 155/183 (84.70%), Postives = 171/183 (93.44%), Query Frame = 1


Query: 1   MPMNSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRD 60
           MP+N N L+LQY+V KKKFLREPSRLFSFK+RQSSGLR+ALQPS EEIKQIF  FDTN+D
Sbjct: 1   MPINPNFLELQYNVSKKKFLREPSRLFSFKDRQSSGLRRALQPSSEEIKQIFNKFDTNKD 60

Query: 61  GRISKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLRS--GVQTREVE 120
           GRISKHEYRGIL+ALGRG+SMEEVQKIF+AVD+DGDGYINLNEFMEV RS  GVQ +EVE
Sbjct: 61  GRISKHEYRGILKALGRGNSMEEVQKIFRAVDSDGDGYINLNEFMEVHRSGGGVQAKEVE 120

Query: 121 SAFKTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMT 180
            AFKTFD+NGD+KISAEEVMRVLKGLGEKCSIEDCRRMVRAVD +GDGMVD++EFMTMMT
Sbjct: 121 FAFKTFDLNGDRKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDSDGDGMVDINEFMTMMT 180

Query: 181 RSA 182
           RSA
Sbjct: 181 RSA 183

[Close]

BLAST of CmaCh19G006090.1 vs. NCBI nr
Match: gi|764562626|ref|XP_011461491.1| (PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-57
Identity = 112/179 (62.57%), Postives = 148/179 (82.68%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRI 63
           N + LD QY++ K+KFLR P+RLFS ++RQ+SGL    +P++ E+K++F+ FDTN+DG+I
Sbjct: 3   NMSFLDFQYNLSKRKFLRTPTRLFSSRDRQNSGLTPGFEPNLNEMKRVFEKFDTNKDGKI 62

Query: 64  SKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLR--SGVQTREVESAF 123
           S+ +YR IL ALG+GS + EV KIFK VD DGDG+IN  EFMEV +   GV+T ++++AF
Sbjct: 63  SQQDYRAILRALGKGSMVGEVPKIFKLVDLDGDGFINFKEFMEVHKKSGGVKTIDIQNAF 122

Query: 124 KTFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRS 181
           +TFD+NGD KISAEEVM VLK LGE+CS+E+C+RMVRAVD +GDGMVD+DEFMTMMTRS
Sbjct: 123 QTFDLNGDGKISAEEVMEVLKRLGERCSLEECQRMVRAVDTDGDGMVDLDEFMTMMTRS 181

[Close]

BLAST of CmaCh19G006090.1 vs. NCBI nr
Match: gi|1009150194|ref|XP_015892888.1| (PREDICTED: calmodulin-like protein 1 [Ziziphus jujuba])

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-56
Identity = 112/179 (62.57%), Postives = 147/179 (82.12%), Query Frame = 1


Query: 6   NLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRISK 65
           + LD QY++ K+KFLR+P+RLFS ++RQSSGL    QP+  E++Q+F  FD N+DG+IS+
Sbjct: 5   SFLDFQYNISKRKFLRKPTRLFSSQDRQSSGLT-TFQPNQAEMRQVFDKFDANKDGKISQ 64

Query: 66  HEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEVLRSG--VQTREVESAFKT 125
           HEY+GIL ALGR +++E+V KIF+  D DGDG+IN  EFMEV + G  V+T ++++AF+T
Sbjct: 65  HEYKGILRALGRTNTIEQVPKIFQIADLDGDGFINFKEFMEVQKKGGGVKTIDIQNAFRT 124

Query: 126 FDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRSAN 183
           FD+NGD KISA EV+ VLK LGE+CS+EDC+RMVRAVD +GDGMVDMDEFMTMMTRS N
Sbjct: 125 FDLNGDGKISAVEVLEVLKRLGERCSLEDCQRMVRAVDIDGDGMVDMDEFMTMMTRSLN 182

[Close]

BLAST of CmaCh19G006090.1 vs. NCBI nr
Match: gi|657963113|ref|XP_008373157.1| (PREDICTED: calmodulin-like protein 1 [Malus domestica])

HSP 1 Score: 226.9 bits (577), Expect = 3.4e-56
Identity = 107/178 (60.11%), Postives = 147/178 (82.58%), Query Frame = 1


Query: 4   NSNLLDLQYSVLKKKFLREPSRLFSFKNRQSSGLRQALQPSMEEIKQIFKWFDTNRDGRI 63
           N+N LD QY++ K+KFLR+PS++FSF++RQ SG+    +P+  E+K++F+ FD+N+DG+I
Sbjct: 3   NANFLDFQYNLSKRKFLRKPSQMFSFRDRQYSGIAPTFEPNPNEMKKVFEKFDSNKDGKI 62

Query: 64  SKHEYRGILEALGRGSSMEEVQKIFKAVDTDGDGYINLNEFMEV-LRSGVQTREVESAFK 123
           S+ EY+ IL ALG+G+ + EV KIFK VD D DG+IN  EFMEV  + G++T ++++AF+
Sbjct: 63  SQAEYKAILRALGKGNMIGEVPKIFKFVDLDEDGFINFKEFMEVHKKGGIRTMDIQNAFR 122

Query: 124 TFDVNGDQKISAEEVMRVLKGLGEKCSIEDCRRMVRAVDRNGDGMVDMDEFMTMMTRS 181
            FD+NGD KISAEEVM VL+GL E+C++EDCRRMVRAVD +GDGMVD+DEFMTMMTRS
Sbjct: 123 MFDLNGDGKISAEEVMEVLRGLEERCNLEDCRRMVRAVDTDGDGMVDLDEFMTMMTRS 180

The following BLAST results are available for this feature:

BLAST of CmaCh19G006090.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML1_ARATH	1.4e-46	52.20	Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1	[more]
CML25_ARATH	1.1e-27	42.75	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]
CML23_ARATH	1.1e-27	43.57	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=...	[more]
CML27_ARATH	1.1e-27	44.85	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana GN=CML27 PE=1 SV=...	[more]
CML24_ARATH	2.5e-27	37.58	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]

BLAST of CmaCh19G006090.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K804_CUCSA	8.8e-80	84.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073720 PE=4 SV=1	[more]
M5VNS5_PRUPE	9.8e-55	58.66	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011990mg PE=4 SV=1	[more]
B9HS04_POPTR	2.2e-54	58.24	Calmodulin-related family protein OS=Populus trichocarpa GN=POPTR_0009s10530g PE...	[more]
V4SBR8_9ROSI	1.3e-51	56.98	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10029360mg PE=4 SV=1	[more]
A0A067F0V1_CITSI	1.3e-51	56.98	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041237mg PE=4 SV=1	[more]

BLAST of CmaCh19G006090.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G15680.1	8.0e-48	52.20	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	6.3e-29	42.75	EF hand calcium-binding protein family	[more]
AT1G18210.1	6.3e-29	44.85	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	6.3e-29	43.57	calmodulin like 23	[more]
AT5G37770.1	1.4e-28	37.58	EF hand calcium-binding protein family	[more]

BLAST of CmaCh19G006090.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109907\|ref\|XP_008454944.1\|	8.8e-81	85.79	PREDICTED: calmodulin-like protein 1 [Cucumis melo]	[more]
gi\|778725125\|ref\|XP_004137114.2\|	1.3e-79	84.70	PREDICTED: calmodulin-like protein 1 [Cucumis sativus]	[more]
gi\|764562626\|ref\|XP_011461491.1\|	1.0e-57	62.57	PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca]	[more]
gi\|1009150194\|ref\|XP_015892888.1\|	1.2e-56	62.57	PREDICTED: calmodulin-like protein 1 [Ziziphus jujuba]	[more]
gi\|657963113\|ref\|XP_008373157.1\|	3.4e-56	60.11	PREDICTED: calmodulin-like protein 1 [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh19G006090	CmaCh19G006090	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh19G006090.1	CmaCh19G006090.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh19G006090.1.exon.1	CmaCh19G006090.1.exon.1	exon
CmaCh19G006090.1.exon.2	CmaCh19G006090.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh19G006090.1.CDS.1	CmaCh19G006090.1.CDS.1	CDS
CmaCh19G006090.1.CDS.2	CmaCh19G006090.1.CDS.2	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 118..177 score: 5.8E-12coord: 46..108 score: 6.8
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 47..75 score: 1.3E-4coord: 152..180 score: 0.0017coord: 83..111 score: 3.0E-7coord: 116..144 score: 3.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 116..147 score: 12.868coord: 148..183 score: 13.37coord: 43..78 score: 13.816coord: 79..114 score: 15
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 44..180 score: 8.1
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 42..178 score: 1.49
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 125..137 score: -coord: 92..104 score: -coord: 161..173 score: -coord: 56..68 scor
None	No IPR available	PRINTS	PR00450	RECOVERIN	coord: 51..72 score: 5.6E-5coord: 97..115 score: 5.6E-5coord: 75..94 score: 5.
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 42..181 score: 2.3
None	No IPR available	PANTHER	PTHR10891:SF533	SUBFAMILY NOT NAMED	coord: 42..181 score: 2.3