CmaCh18G006290.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh18G006290.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Ribonuclease T2 family protein
Location	Cma_Chr18 : 5605033 .. 5605424 (-)
Sequence length	333

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATATGTTCGGAGTCCGTTTTTAATCAAGTTCAATACTTTCAAACAACTTTGAACATTCGTACCAATTCCAACTACGACCTACTGGCCATTCTGAAAACTGCCAGGCTCAGCCCAACCGGCAGCATATCCCACCGCTATCATGTCATCGAAGGTGCCATTCAGGCTGCAATAGGAAAGAAGTCAGGCCTCCACTGCAACATCAATAAGAAAACTAGAAATAATCAATTGCACGAAATTGTATTATGCTTTGATAAAAATGGAGTGACTCTCATAGATTGTCCGTTTCCCCGCAAGTGCCCAACGCAGTTTAAGGCTCTGGTTTTAATACTTGTTTTTTTTTTTTTCTTCTTGCACTCTAAGATGTCCATTTATGCTATGGAAAAATAA

mRNA sequence

ATGGAATATGTTCGGATTCAATACTTTCAAACAACTTTGAACATTCGTACCAATTCCAACTACGACCTACTGGCCATTCTGAAAACTGCCAGGCTCAGCCCAACCGGCAGCATATCCCACCGCTATCATGTCATCGAAGGTGCCATTCAGGCTGCAATAGGAAAGAAGTCAGGCCTCCACTGCAACATCAATAAGAAAACTAGAAATAATCAATTGCACGAAATTGTATTATGCTTTGATAAAAATGGAGTGACTCTCATAGATTGTCCGTTTCCCCGCAAGTGCCCAACGCAGTTTAAGGCTCTGATGTCCATTTATGCTATGGAAAAATAA

Coding sequence (CDS)

ATGGAATATGTTCGGATTCAATACTTTCAAACAACTTTGAACATTCGTACCAATTCCAACTACGACCTACTGGCCATTCTGAAAACTGCCAGGCTCAGCCCAACCGGCAGCATATCCCACCGCTATCATGTCATCGAAGGTGCCATTCAGGCTGCAATAGGAAAGAAGTCAGGCCTCCACTGCAACATCAATAAGAAAACTAGAAATAATCAATTGCACGAAATTGTATTATGCTTTGATAAAAATGGAGTGACTCTCATAGATTGTCCGTTTCCCCGCAAGTGCCCAACGCAGTTTAAGGCTCTGATGTCCATTTATGCTATGGAAAAATAA

Protein sequence

MEYVRIQYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKTRNNQLHEIVLCFDKNGVTLIDCPFPRKCPTQFKALMSIYAMEK

Homology

[Close]

BLAST of CmaCh18G006290.1 vs. Swiss-Prot
Match: RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-07
Identity = 35/95 (36.84%), Postives = 60/95 (63.16%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF+  L+++  +  DLL+IL+ A + P G  S+    I  AI++AIG    + CN+++ +
Sbjct: 136 YFKKALDLK--NQIDLLSILQGADIHPDGE-SYDLVNIRNAIKSAIGYTPWIQCNVDQ-S 195

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP-FP-RKCPTQFK 101
            N+QL+++ +C D +G +LI+CP FP  KC T  +
Sbjct: 196 GNSQLYQVYICVDGSGSSLIECPIFPGGKCGTSIE 226

[Close]

BLAST of CmaCh18G006290.1 vs. Swiss-Prot
Match: RNMC_MOMCH (Ribonuclease MC OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-07
Identity = 29/82 (35.37%), Postives = 48/82 (58.54%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF+  +++R  +NYD++  L+     P G    R   I+G ++A  GK  GL C  + +T
Sbjct: 102 YFKLAVDMR--NNYDIIGALRPHAAGPNGRTKSR-QAIKGFLKAKFGKFPGLRCRTDPQT 161

Query: 68  RNNQLHEIVLCFDKNGVTLIDC 90
           + + L ++V CF ++G TLIDC
Sbjct: 162 KVSYLVQVVACFAQDGSTLIDC 180

[Close]

BLAST of CmaCh18G006290.1 vs. Swiss-Prot
Match: RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.5e-07
Identity = 35/96 (36.46%), Postives = 56/96 (58.33%), Query Frame = 1


Query: 7   QYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKK 66
           +YFQT LN++  +N  LL  L  A ++P G  S+    I  +I+ +IG    + CN    
Sbjct: 135 EYFQTALNLKQKTN--LLGALTKAGINPDGK-SYSLESIRDSIKESIGFTPWVECN-RDG 194

Query: 67  TRNNQLHEIVLCFDKNGVTLIDCP-FPR-KCPTQFK 101
           + N+QL+++ LC D++G  LI+CP FP  KC  + +
Sbjct: 195 SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIE 226

[Close]

BLAST of CmaCh18G006290.1 vs. TrEMBL
Match: A0A0A0LDS5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G743990 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 3.7e-13
Identity = 49/99 (49.49%), Postives = 58/99 (58.59%), Query Frame = 1


Query: 7   QYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCN-INK 66
           QYF+  LNIR    YDL+AIL  A L P+ S  H+Y  I   IQAA+  K  L CN  N 
Sbjct: 120 QYFELALNIRKYKKYDLMAILNNAGLHPSTSKLHQYDDIADLIQAAVEAKPLLKCNDKNG 179

Query: 67  KTRNNQLHEIVLCFDKNGVTLIDC---PFPRK-CPTQFK 101
           + +NNQL E++LCFD  GV  IDC   P P K C   FK
Sbjct: 180 QGQNNQLWEVILCFDHGGVNPIDCPAQPVPHKMCVGDFK 218

[Close]

BLAST of CmaCh18G006290.1 vs. TrEMBL
Match: A0A0A0LAN6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-10
Identity = 41/84 (48.81%), Postives = 51/84 (60.71%), Query Frame = 1


Query: 7   QYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKK 66
           QYFQ +LN+     +DLL ILK A L+P  S +     I   IQ    K  G+ CN N K
Sbjct: 122 QYFQISLNLLRK--FDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVK 181

Query: 67  TRNNQLHEIVLCFDKNGVTLIDCP 91
           T  +QL+EIVLC +K+G TLIDCP
Sbjct: 182 TGKSQLNEIVLCLEKDGATLIDCP 203

[Close]

BLAST of CmaCh18G006290.1 vs. TrEMBL
Match: A0A0A0LAB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 9.5e-09
Identity = 38/83 (45.78%), Postives = 45/83 (54.22%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF  +L  R N  YDLL IL  A LS           +   I+ A G + G+ CNIN  T
Sbjct: 123 YFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGIT 182

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP 91
           +  QL EIVLCF  +G TLIDCP
Sbjct: 183 KKLQLKEIVLCFGNDGTTLIDCP 205

[Close]

BLAST of CmaCh18G006290.1 vs. TrEMBL
Match: G7KFA3_MEDTR (Ribonuclease T2 family protein OS=Medicago truncatula GN=MTR_5g040960 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-08
Identity = 38/87 (43.68%), Postives = 56/87 (64.37%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF+TTLN+R  +N  LL  L +A + P G  S+    I+GAIQ AIG    + CN++  +
Sbjct: 135 YFETTLNLRQKAN--LLQALTSAGIQPDGG-SYTLSSIKGAIQNAIGYTPYIECNVD-SS 194

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP-FPR 94
           +N+QL+++ LC D +G   IDCP FP+
Sbjct: 195 KNSQLYQVYLCVDTSGSDFIDCPVFPK 217

[Close]

BLAST of CmaCh18G006290.1 vs. TrEMBL
Match: G7KFA1_MEDTR (Ribonuclease T2 family protein OS=Medicago truncatula GN=MTR_5g040940 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.1e-08
Identity = 39/95 (41.05%), Postives = 60/95 (63.16%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF+TTLN+R  +N  LL  L +A + P G+ S+    I+GAIQ A+G    + CN++  +
Sbjct: 134 YFETTLNLRQKAN--LLQALTSAGVQPDGN-SYSLSSIKGAIQNAVGFAPFIECNVD-SS 193

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP-FPR-KCPTQFK 101
            N+QL+++ LC D +G   IDCP FP  KC ++ +
Sbjct: 194 GNSQLYQVYLCVDTSGSNFIDCPVFPHGKCGSEIE 224

[Close]

BLAST of CmaCh18G006290.1 vs. TAIR10
Match: AT2G02990.1 (AT2G02990.1 ribonuclease 1)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-09
Identity = 35/96 (36.46%), Postives = 56/96 (58.33%), Query Frame = 1


Query: 7   QYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKK 66
           +YFQT LN++  +N  LL  L  A ++P G  S+    I  +I+ +IG    + CN    
Sbjct: 135 EYFQTALNLKQKTN--LLGALTKAGINPDGK-SYSLESIRDSIKESIGFTPWVECN-RDG 194

Query: 67  TRNNQLHEIVLCFDKNGVTLIDCP-FPR-KCPTQFK 101
           + N+QL+++ LC D++G  LI+CP FP  KC  + +
Sbjct: 195 SGNSQLYQVYLCVDRSGSGLIECPVFPHGKCGAEIE 226

[Close]

BLAST of CmaCh18G006290.1 vs. NCBI nr
Match: gi|700203881|gb|KGN59014.1| (hypothetical protein Csa_3G743990 [Cucumis sativus])

HSP 1 Score: 82.4 bits (202), Expect = 5.4e-13
Identity = 49/99 (49.49%), Postives = 58/99 (58.59%), Query Frame = 1


Query: 7   QYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCN-INK 66
           QYF+  LNIR    YDL+AIL  A L P+ S  H+Y  I   IQAA+  K  L CN  N 
Sbjct: 120 QYFELALNIRKYKKYDLMAILNNAGLHPSTSKLHQYDDIADLIQAAVEAKPLLKCNDKNG 179

Query: 67  KTRNNQLHEIVLCFDKNGVTLIDC---PFPRK-CPTQFK 101
           + +NNQL E++LCFD  GV  IDC   P P K C   FK
Sbjct: 180 QGQNNQLWEVILCFDHGGVNPIDCPAQPVPHKMCVGDFK 218

[Close]

BLAST of CmaCh18G006290.1 vs. NCBI nr
Match: gi|700203887|gb|KGN59020.1| (hypothetical protein Csa_3G745530 [Cucumis sativus])

HSP 1 Score: 73.9 bits (180), Expect = 1.9e-10
Identity = 41/84 (48.81%), Postives = 51/84 (60.71%), Query Frame = 1


Query: 7   QYFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKK 66
           QYFQ +LN+     +DLL ILK A L+P  S +     I   IQ    K  G+ CN N K
Sbjct: 122 QYFQISLNLLRK--FDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNKNVK 181

Query: 67  TRNNQLHEIVLCFDKNGVTLIDCP 91
           T  +QL+EIVLC +K+G TLIDCP
Sbjct: 182 TGKSQLNEIVLCLEKDGATLIDCP 203

[Close]

BLAST of CmaCh18G006290.1 vs. NCBI nr
Match: gi|778683692|ref|XP_011651927.1| (PREDICTED: ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-08
Identity = 38/83 (45.78%), Postives = 45/83 (54.22%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF  +L  R N  YDLL IL  A LS           +   I+ A G + G+ CNIN  T
Sbjct: 123 YFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCNINGIT 182

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP 91
           +  QL EIVLCF  +G TLIDCP
Sbjct: 183 KKLQLKEIVLCFGNDGTTLIDCP 205

[Close]

BLAST of CmaCh18G006290.1 vs. NCBI nr
Match: gi|357487007|ref|XP_003613791.1| (ribonuclease T2 family protein [Medicago truncatula])

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-08
Identity = 38/87 (43.68%), Postives = 56/87 (64.37%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF+TTLN+R  +N  LL  L +A + P G  S+    I+GAIQ AIG    + CN++  +
Sbjct: 135 YFETTLNLRQKAN--LLQALTSAGIQPDGG-SYTLSSIKGAIQNAIGYTPYIECNVD-SS 194

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP-FPR 94
           +N+QL+++ LC D +G   IDCP FP+
Sbjct: 195 KNSQLYQVYLCVDTSGSDFIDCPVFPK 217

[Close]

BLAST of CmaCh18G006290.1 vs. NCBI nr
Match: gi|357487003|ref|XP_003613789.1| (ribonuclease T2 family protein [Medicago truncatula])

HSP 1 Score: 66.6 bits (161), Expect = 3.0e-08
Identity = 39/95 (41.05%), Postives = 60/95 (63.16%), Query Frame = 1


Query: 8   YFQTTLNIRTNSNYDLLAILKTARLSPTGSISHRYHVIEGAIQAAIGKKSGLHCNINKKT 67
           YF+TTLN+R  +N  LL  L +A + P G+ S+    I+GAIQ A+G    + CN++  +
Sbjct: 134 YFETTLNLRQKAN--LLQALTSAGVQPDGN-SYSLSSIKGAIQNAVGFAPFIECNVD-SS 193

Query: 68  RNNQLHEIVLCFDKNGVTLIDCP-FPR-KCPTQFK 101
            N+QL+++ LC D +G   IDCP FP  KC ++ +
Sbjct: 194 GNSQLYQVYLCVDTSGSNFIDCPVFPHGKCGSEIE 224

The following BLAST results are available for this feature:

BLAST of CmaCh18G006290.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RNLE_SOLLC	1.2e-07	36.84	Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2	[more]
RNMC_MOMCH	1.5e-07	35.37	Ribonuclease MC OS=Momordica charantia PE=1 SV=1	[more]
RNS1_ARATH	1.5e-07	36.46	Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1	[more]

BLAST of CmaCh18G006290.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDS5_CUCSA	3.7e-13	49.49	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G743990 PE=3 SV=1	[more]
A0A0A0LAN6_CUCSA	1.3e-10	48.81	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G745530 PE=3 SV=1	[more]
A0A0A0LAB0_CUCSA	9.5e-09	45.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G736680 PE=3 SV=1	[more]
G7KFA3_MEDTR	1.2e-08	43.68	Ribonuclease T2 family protein OS=Medicago truncatula GN=MTR_5g040960 PE=2 SV=1	[more]
G7KFA1_MEDTR	2.1e-08	41.05	Ribonuclease T2 family protein OS=Medicago truncatula GN=MTR_5g040940 PE=3 SV=1	[more]

BLAST of CmaCh18G006290.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G02990.1	8.5e-09	36.46	ribonuclease 1	[more]

BLAST of CmaCh18G006290.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700203881\|gb\|KGN59014.1\|	5.4e-13	49.49	hypothetical protein Csa_3G743990 [Cucumis sativus]	[more]
gi\|700203887\|gb\|KGN59020.1\|	1.9e-10	48.81	hypothetical protein Csa_3G745530 [Cucumis sativus]	[more]
gi\|778683692\|ref\|XP_011651927.1\|	1.4e-08	45.78	PREDICTED: ribonuclease MC-like [Cucumis sativus]	[more]
gi\|357487007\|ref\|XP_003613791.1\|	1.8e-08	43.68	ribonuclease T2 family protein [Medicago truncatula]	[more]
gi\|357487003\|ref\|XP_003613789.1\|	3.0e-08	41.05	ribonuclease T2 family protein [Medicago truncatula]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001568	RNase_T2-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0033897	ribonuclease T2 activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh18G006290	CmaCh18G006290	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh18G006290.1	CmaCh18G006290.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh18G006290.1.CDS.3	CmaCh18G006290.1.CDS.3	CDS
CmaCh18G006290.1.CDS.2	CmaCh18G006290.1.CDS.2	CDS
CmaCh18G006290.1.CDS.1	CmaCh18G006290.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh18G006290.1.exon.3	CmaCh18G006290.1.exon.3	exon
CmaCh18G006290.1.exon.2	CmaCh18G006290.1.exon.2	exon
CmaCh18G006290.1.exon.1	CmaCh18G006290.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	GENE3D	G3DSA:3.90.730.10		coord: 7..101 score: 6.8
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 6..89 score: 2.2
IPR001568	Ribonuclease T2-like	unknown	SSF55895	Ribonuclease Rh-like	coord: 6..98 score: 7.59