CmaCh16G008210.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh16G008210.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSesquiterpene synthase
LocationCma_Chr16 : 4572537 .. 4572950 (+)
Sequence length261
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAACCAAAGTAATCACCATTTCTTCAATTTTAGATGATATATATAATGCTTATGGAAGAACTCCAAGTCCTCCCTCCCGCTATTCAAAGGGTCACTTAAATTCATTCAAATTGATGAAGATTTTAGCTTTAAAAGTTTGGCTTACTTAGTACTAACTTTTTTTTTCATCGTACTACACACTGTTTAAAAACCCCATATAAATTTTTTATATGGGAATAATCCATATTTAAAATAAAGTTGAAGATTTTATTAACAATGATGTCATTAAATTTTCAAGAAGAAGCAGAATCAATGTACTCCATGAGTACGTGAAACCTTTTTCTATAGCAAAGTTAGATCTTTATAAGGAAATTAGTAAAGAGATAGACAAGAATGAAAGTTCACTCACCAGAAGGAGAGGTACACGGTAA

mRNA sequence

ATGTTAACCAAAGTAATCACCATTTCTTCAATTTTAGATGATATATATAATGCTTATGGAAGAACTCCAAGTCCTCCCTCCCGCTATTCAAAGGATTTTATTAACAATGATGTCATTAAATTTTCAAGAAGAAGCAGAATCAATGTACTCCATGAGTACGTGAAACCTTTTTCTATAGCAAAGTTAGATCTTTATAAGGAAATTAGTAAAGAGATAGACAAGAATGAAAGTTCACTCACCAGAAGGAGAGGTACACGGTAA

Coding sequence (CDS)

ATGTTAACCAAAGTAATCACCATTTCTTCAATTTTAGATGATATATATAATGCTTATGGAAGAACTCCAAGTCCTCCCTCCCGCTATTCAAAGGATTTTATTAACAATGATGTCATTAAATTTTCAAGAAGAAGCAGAATCAATGTACTCCATGAGTACGTGAAACCTTTTTCTATAGCAAAGTTAGATCTTTATAAGGAAATTAGTAAAGAGATAGACAAGAATGAAAGTTCACTCACCAGAAGGAGAGGTACACGGTAA

Protein sequence

MLTKVITISSILDDIYNAYGRTPSPPSRYSKDFINNDVIKFSRRSRINVLHEYVKPFSIAKLDLYKEISKEIDKNESSLTRRRGTR
BLAST of CmaCh16G008210.1 vs. Swiss-Prot
Match: GCSY1_CUCME ((+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-10
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = 1

Query: 1   MLTKVITISSILDDIYNAYGRTPSPPSRYSKDFINNDVIKFSRRSRINVLHEYVKPFSIA 60
           MLTKVI I+SILDDIY+AYG        + +  +    I+   RS ++ L  Y+KPF +A
Sbjct: 311 MLTKVIAIASILDDIYDAYGT-------FEELQVLTPAIQRWDRSMVHTLPLYMKPFYVA 370

Query: 61  KLDLYKEISKEIDKNESSL 80
            L+LY+EI KEIDK+++SL
Sbjct: 371 MLELYEEIGKEIDKDQNSL 382

BLAST of CmaCh16G008210.1 vs. Swiss-Prot
Match: TPSVS_VITVI (Valencene synthase OS=Vitis vinifera GN=ValCS PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.0e-06
Identity = 32/74 (43.24%), Postives = 47/74 (63.51%), Query Frame = 1

Query: 1   MLTKVITISSILDDIYNAYGRTPSPPSRYSKDFINNDVIKFSRRSRINVLHEYVKPFSIA 60
           +LTKVI ++SILDDI++AYG TP     + +     D+      + IN L EY+K   +A
Sbjct: 298 ILTKVIAMTSILDDIHDAYG-TPEELKLFIEAIERWDI------NSINQLPEYMKLCYVA 357

Query: 61  KLDLYKEISKEIDK 75
            LD+YKEI +E++K
Sbjct: 358 LLDVYKEIEEEMEK 364

BLAST of CmaCh16G008210.1 vs. TrEMBL
Match: A0A0A0L2I8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-08
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = 1

Query: 1   MLTKVITISSILDDIYNAYGRTPSPPSRYSKDFINNDVIKFSRRSRINVLHEYVKPFSIA 60
           MLTKVI ISSILDDIY+AYG        + +  +    I+   RS ++ L EY+KPF +A
Sbjct: 298 MLTKVIAISSILDDIYDAYGT-------FEELQVLAPAIQRWDRSMVHTLPEYMKPFYVA 357

Query: 61  KLDLYKEISKEIDKNESSL 80
             ++Y+EISKEI K+++SL
Sbjct: 358 MFEVYEEISKEIGKDQNSL 369

BLAST of CmaCh16G008210.1 vs. NCBI nr
Match: gi|661902982|ref|NP_001284382.1| ((+)-gamma-cadinene synthase [Cucumis melo])

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-08
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = 1

Query: 1   MLTKVITISSILDDIYNAYGRTPSPPSRYSKDFINNDVIKFSRRSRINVLHEYVKPFSIA 60
           MLTKVI I+SILDDIY+AYG        + +  +    I+   RS ++ L  Y+KPF +A
Sbjct: 311 MLTKVIAIASILDDIYDAYGT-------FEELQVLTPAIQRWDRSMVHTLPLYMKPFYVA 370

Query: 61  KLDLYKEISKEIDKNESSL 80
            L+LY+EI KEIDK+++SL
Sbjct: 371 MLELYEEIGKEIDKDQNSL 382

BLAST of CmaCh16G008210.1 vs. NCBI nr
Match: gi|778676208|ref|XP_011650545.1| (PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus])

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-08
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = 1

Query: 1   MLTKVITISSILDDIYNAYGRTPSPPSRYSKDFINNDVIKFSRRSRINVLHEYVKPFSIA 60
           MLTKVI ISSILDDIY+AYG        + +  +    I+   RS ++ L EY+KPF +A
Sbjct: 298 MLTKVIAISSILDDIYDAYGT-------FEELQVLAPAIQRWDRSMVHTLPEYMKPFYVA 357

Query: 61  KLDLYKEISKEIDKNESSL 80
             ++Y+EISKEI K+++SL
Sbjct: 358 MFEVYEEISKEIGKDQNSL 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GCSY1_CUCME1.1e-1048.10(+)-gamma-cadinene synthase OS=Cucumis melo PE=1 SV=1[more]
TPSVS_VITVI5.0e-0643.24Valencene synthase OS=Vitis vinifera GN=ValCS PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2I8_CUCSA1.7e-0848.10Uncharacterized protein OS=Cucumis sativus GN=Csa_3G097540 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|661902982|ref|NP_001284382.1|1.8e-0848.10(+)-gamma-cadinene synthase [Cucumis melo][more]
gi|778676208|ref|XP_011650545.1|2.4e-0848.10PREDICTED: (+)-gamma-cadinene synthase [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR005630Terpene_synthase_metal-bd
IPR008949Isoprenoid_synthase_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0010333terpene synthase activity
GO:0016829lyase activity
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045338 farnesyl diphosphate metabolic process
biological_process GO:0051762 sesquiterpene biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016829 lyase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0010334 sesquiterpene synthase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh16G008210CmaCh16G008210gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh16G008210.1CmaCh16G008210.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh16G008210.1.exon.1CmaCh16G008210.1.exon.1exon
CmaCh16G008210.1.exon.2CmaCh16G008210.1.exon.2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh16G008210.1.CDS.1CmaCh16G008210.1.CDS.1CDS
CmaCh16G008210.1.CDS.2CmaCh16G008210.1.CDS.2CDS


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 1..78
score: 3.
IPR008949Isoprenoid synthase domainGENE3DG3DSA:1.10.600.10coord: 1..76
score: 1.
IPR008949Isoprenoid synthase domainunknownSSF48576Terpenoid synthasescoord: 1..81
score: 4.8