CmaCh07G002470.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh07G002470.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Heat stress transcription factor B-2a
Location	Cma_Chr07 : 1113316 .. 1114519 (+)
Sequence length	887

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAACCTCTACCCTTTATAGAACGTAACCTGGTAGCTTCCTAACAAATGGCTGCAGGTGGAAGGCTTATAAGTTGAGAAGCTGATCAACTTTGAACAGATTTTGGGAGTGAATTTGATTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTGTCAAAGACTTATGATCTGTTAGAAGAGGCTGGAAGCTTCCATTTAAAAGCAGCAGATCAGGAGGAAGAAGAAGAAGAAGAAGAAGAAAATAGGATTGTGTCCTGGAATGCAGAGGGAAATGGGTTCATAGTTTGGTCTCCTGCGGAATTCTCTGAGGTGTTGTTGCCTAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTACGTATGTATTCTCTCTTTCTCTATCTTTTTGAAAATGCCCCCACCACTTTTTATTCTCTGAAACATCTTGAAGAAGAATTATTCATTATAATGAGGATCCAATTGACCACTTTCCTGATTCGAATGCTTAAATTCTGCCATAGAAATTGGATCTAATAGGTAATATGTTATCTATTTGATGTGTTCCTTATGCTCCCTCTCACATAGGTTTGGGCATGAGTACCCATGGATATTAAATGAAAAAGGAAATGAGATTTTTGATAGGTTATGATATATTAGAGTCGAGTTTCAATCTAATTATTGTAATAATGGAAACAGGGGTTCAAGAAGACGTCGCAAAAGAGGTGGGAATTCAAGCATGAGAAGTTTCAGAGGGGAAACAAGCAGATGCTGGGGGAGATTTTACGTAAGAAATGTGAGCCTAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGATCTGAAACAATGGCGGTTGGTCAGAAGAATGGCGACCATCTGCAACTAATGGAAGAGAACAAGAATCTAAGAAGACGGAAGCTGGAATTGCAAGCTCGAATAGTCCGATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGATCAGTGCATTAAGAAGTAGCCTACAGAGTTTTGCTAATTAGAAAATTGGGTTGGTTGTGAAAATTTGAGGAAGAAAGTAATACTTCCGGGTTATGCATTTGCTCCAAATTGAGTTTCCATTGTCCACTCTAATTAGTTCTAGGGAAGTTGCTATCTGTAAGAGTTGATATGAACAATGAACAATTAACAAAACAGTTTATACTGGTTTATAAACTAA

mRNA sequence

GAAACCTCTACCCTTTATAGAACGTAACCTGGTAGCTTCCTAACAAATGGCTGCAGGTGGAAGGCTTATAAGTTGAGAAGCTGATCAACTTTGAACAGATTTTGGGAGTGAATTTGATTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTGTCAAAGACTTATGATCTGTTAGAAGAGGCTGGAAGCTTCCATTTAAAAGCAGCAGATCAGGAGGAAGAAGAAGAAGAAGAAGAAGAAAATAGGATTGTGTCCTGGAATGCAGAGGGAAATGGGTTCATAGTTTGGTCTCCTGCGGAATTCTCTGAGGTGTTGTTGCCTAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTACGGGTTCAAGAAGACGTCGCAAAAGAGGTGGGAATTCAAGCATGAGAAGTTTCAGAGGGGAAACAAGCAGATGCTGGGGGAGATTTTACGTAAGAAATGTGAGCCTAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGATCTGAAACAATGGCGGTTGGTCAGAAGAATGGCGACCATCTGCAACTAATGGAAGAGAACAAGAATCTAAGAAGACGGAAGCTGGAATTGCAAGCTCGAATAGTCCGATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGATCAGTGCATTAAGAAGTAGCCTACAGAGTTTTGCTAATTAGAAAATTGGGTTGGTTGTGAAAATTTGAGGAAGAAAGTAATACTTCCGGGTTATGCATTTGCTCCAAATTGAGTTTCCATTGTCCACTCTAATTAGTTCTAGGGAAGTTGCTATCTGTAAGAGTTGATATGAACAATGAACAATTAACAAAACAGTTTATACTGGTTTATAAACTAA

Coding sequence (CDS)

ATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTGTCAAAGACTTATGATCTGTTAGAAGAGGCTGGAAGCTTCCATTTAAAAGCAGCAGATCAGGAGGAAGAAGAAGAAGAAGAAGAAGAAAATAGGATTGTGTCCTGGAATGCAGAGGGAAATGGGTTCATAGTTTGGTCTCCTGCGGAATTCTCTGAGGTGTTGTTGCCTAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTACGGGTTCAAGAAGACGTCGCAAAAGAGGTGGGAATTCAAGCATGAGAAGTTTCAGAGGGGAAACAAGCAGATGCTGGGGGAGATTTTACGTAAGAAATGTGAGCCTAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGATCTGAAACAATGGCGGTTGGTCAGAAGAATGGCGACCATCTGCAACTAATGGAAGAGAACAAGAATCTAAGAAGACGGAAGCTGGAATTGCAAGCTCGAATAGTCCGATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGATCAGTGCATTAAGAAGTAG

Protein sequence

MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRLLDCLDQCIKK

Homology

[Close]

BLAST of CmaCh07G002470.1 vs. Swiss-Prot
Match: HFB2A_ARATH (Heat stress transcription factor B-2a OS=Arabidopsis thaliana GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.0e-28
Identity = 71/202 (35.15%), Postives = 110/202 (54.46%), Query Frame = 1


Query: 3   SSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAE 62
           SS+   P PFL+KT++L+E++                   + ++SWN +G+ FIVW+P +
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSI-----------------DDVISWNEDGSSFIVWNPTD 74

Query: 63  FSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEP 122
           F++ LLPK+FKHNNFSSF+RQLNTYGFKK    RWEF ++ F+RG K++L EI R+K   
Sbjct: 75  FAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITT 134

Query: 123 SVFPAFLRSSHEGSETMAVGQKN-----------------------------GDHLQLME 176
           +       SS + ++TM V   N                             G  ++L+E
Sbjct: 135 THQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLE 194

[Close]

BLAST of CmaCh07G002470.1 vs. Swiss-Prot
Match: HFA1B_ARATH (Heat stress transcription factor A-1b OS=Arabidopsis thaliana GN=HSFA1B PE=2 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 70/163 (42.94%), Postives = 96/163 (58.90%), Query Frame = 1


Query: 11  PFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPK 70
           PFLSKTYD++++  +                 N +VSW++  N F+VWS  EFS+VLLPK
Sbjct: 27  PFLSKTYDMVDDPLT-----------------NEVVSWSSGNNSFVVWSAPEFSKVLLPK 86

Query: 71  YFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAFLR 130
           YFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG KQ+L  I+R+K  PS      +
Sbjct: 87  YFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRK--PSHVQQNQQ 146

Query: 131 SSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFK 174
            +   S ++    + G    + EE + L+R K  L   +VR +
Sbjct: 147 QTQVQSSSVGACVEVG-KFGIEEEVERLKRDKNVLMQELVRLR 169

[Close]

BLAST of CmaCh07G002470.1 vs. Swiss-Prot
Match: HFA1A_ARATH (Heat stress transcription factor A-1a OS=Arabidopsis thaliana GN=HSFA1A PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 75/174 (43.10%), Postives = 97/174 (55.75%), Query Frame = 1


Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++E+  +                 + IVSW+   N FIVW P EFS  LL
Sbjct: 50  PPPFLSKTYDMVEDPAT-----------------DAIVSWSPTNNSFIVWDPPEFSRDLL 109

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAF 128
           PKYFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L +I R+K   SV    
Sbjct: 110 PKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRK---SVQGHG 169

Query: 129 LRSSHEGSETMAVGQKNGDHLQ---------LMEENKNLRRRKLELQARIVRFK 174
             SS+  S+ ++ GQ +   L          L EE + L+R K  L   +V+ +
Sbjct: 170 SSSSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLR 203

[Close]

BLAST of CmaCh07G002470.1 vs. Swiss-Prot
Match: HFA1D_ARATH (Heat stress transcription factor A-1d OS=Arabidopsis thaliana GN=HSFA1D PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 70/166 (42.17%), Postives = 89/166 (53.61%), Query Frame = 1


Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++++  +                 + IVSW+A  N FIVW P EF+  LL
Sbjct: 35  PPPFLSKTYDMVDDHNT-----------------DSIVSWSANNNSFIVWKPPEFARDLL 94

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAF 128
           PK FKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L  I R+K         
Sbjct: 95  PKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQGH 154

Query: 129 LRSSHEGSETMAVGQ-KNGDHLQLMEENKNLRRRKLELQARIVRFK 174
            RS H   +  +V          L EE + L+R K  L   +VR +
Sbjct: 155 QRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLR 183

[Close]

BLAST of CmaCh07G002470.1 vs. Swiss-Prot
Match: HSFA5_ARATH (Heat stress transcription factor A-5 OS=Arabidopsis thaliana GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 67/165 (40.61%), Postives = 95/165 (57.58%), Query Frame = 1


Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           PAPFL KTY++++++ +                 ++IVSW+A  N FIVW+ AEFS +LL
Sbjct: 21  PAPFLVKTYEMVDDSST-----------------DQIVSWSANNNSFIVWNHAEFSRLLL 80

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAF 128
           P YFKHNNFSSFIRQLNTYGF+K   +RWEF ++ F +  K +L  I R+K      P  
Sbjct: 81  PTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK------PIH 140

Query: 129 LRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFK 174
             S    S T      + +   L E+   L R K  ++A++++FK
Sbjct: 141 SHSHPPASST------DQERAVLQEQMDKLSREKAAIEAKLLKFK 156

[Close]

BLAST of CmaCh07G002470.1 vs. TrEMBL
Match: A0A0A0KI26_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517310 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 2.6e-83
Identity = 159/197 (80.71%), Postives = 174/197 (88.32%), Query Frame = 1


Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE--------NRIVSWNAEG 60
           MQSS+TRNPAPFLSKTYDLLEEAGSFH + ADQ++EE+EEEE        NR VSWNAEG
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFEEADQDQEEDEEEEEEEEKNGGNRTVSWNAEG 60

Query: 61  NGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQML 120
           +GFIVWSPA+FSE LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG + ML
Sbjct: 61  SGFIVWSPADFSEHLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHML 120

Query: 121 GEILRKKCEPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRF 180
            EI+RKKCEPSVFPAFLRSSHEG  TMAV Q+NGDHL LMEEN NLRR+KLELQA+I +F
Sbjct: 121 VEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQF 180

Query: 181 KALHIRLLDCLDQCIKK 190
           KALHIRLLDCL +CI+K
Sbjct: 181 KALHIRLLDCLGRCIEK 197

[Close]

BLAST of CmaCh07G002470.1 vs. TrEMBL
Match: I1J7X9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_01G143500 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.0e-58
Identity = 118/184 (64.13%), Postives = 139/184 (75.54%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPA 61
           QS + R PAPFL KTYDLLEE G            E  E+  +IVSWNAEG GF+VWSP+
Sbjct: 25  QSPRQRCPAPFLLKTYDLLEELG------------ENIEDSTKIVSWNAEGTGFVVWSPS 84

Query: 62  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCE 121
           EFSE+ LP+YFKHNNFSSFIRQLNTYGFKK S KRWEFKHEKFQRG + MLGEI RKKCE
Sbjct: 85  EFSELTLPRYFKHNNFSSFIRQLNTYGFKKISSKRWEFKHEKFQRGCRHMLGEITRKKCE 144

Query: 122 PSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRLLD 181
           PSVFPA+L+SS E +   +  ++N DH  LMEENKNL++ +LELQ +I   KAL ++LL+
Sbjct: 145 PSVFPAYLKSSSEENNATSSTEENNDHQLLMEENKNLKKERLELQVQIDECKALEMKLLE 196

Query: 182 CLDQ 186
           CL Q
Sbjct: 205 CLSQ 196

[Close]

BLAST of CmaCh07G002470.1 vs. TrEMBL
Match: A0A0B2S611_GLYSO (Heat stress transcription factor B-2a OS=Glycine soja GN=glysoja_028764 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.0e-58
Identity = 118/184 (64.13%), Postives = 139/184 (75.54%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPA 61
           QS + R PAPFL KTYDLLEE G            E  E+  +IVSWNAEG GF+VWSP+
Sbjct: 25  QSPRQRCPAPFLLKTYDLLEELG------------ENIEDSTKIVSWNAEGTGFVVWSPS 84

Query: 62  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCE 121
           EFSE+ LP+YFKHNNFSSFIRQLNTYGFKK S KRWEFKHEKFQRG + MLGEI RKKCE
Sbjct: 85  EFSELTLPRYFKHNNFSSFIRQLNTYGFKKISSKRWEFKHEKFQRGCRHMLGEITRKKCE 144

Query: 122 PSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRLLD 181
           PSVFPA+L+SS E +   +  ++N DH  LMEENKNL++ +LELQ +I   KAL ++LL+
Sbjct: 145 PSVFPAYLKSSSEENNATSSTEENNDHQLLMEENKNLKKERLELQVQIDECKALEMKLLE 196

Query: 182 CLDQ 186
           CL Q
Sbjct: 205 CLSQ 196

[Close]

BLAST of CmaCh07G002470.1 vs. TrEMBL
Match: M5WVD4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011804mg PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.5e-57
Identity = 119/186 (63.98%), Postives = 143/186 (76.88%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPA 61
           QS +TR+PAPFLSKTYDLLEE  +           EE +   +IVSWNAEG+GF+VWSPA
Sbjct: 9   QSPRTRSPAPFLSKTYDLLEEGAA-----------EEGDSGKKIVSWNAEGSGFVVWSPA 68

Query: 62  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCE 121
           EFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS ++WEFKHE+FQ+G + ML EI RKKCE
Sbjct: 69  EFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSPRQWEFKHERFQKGCRHMLVEITRKKCE 128

Query: 122 PSVFPAFLRSSHE--GSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRL 181
           PS FP +L++S E   S T     +  + L LMEENKNLR++KLELQ +I +FKAL ++L
Sbjct: 129 PSAFPVYLKASEESGSSNTAVAAAEENNCLLLMEENKNLRKQKLELQMQISQFKALEMKL 183

Query: 182 LDCLDQ 186
           LDCL Q
Sbjct: 189 LDCLAQ 183

[Close]

BLAST of CmaCh07G002470.1 vs. TrEMBL
Match: W9RZ03_9ROSA (Heat stress transcription factor B-2a OS=Morus notabilis GN=L484_014528 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.2e-57
Identity = 118/189 (62.43%), Postives = 142/189 (75.13%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEE-------ENRIVSWNAEGNG 61
           QS  T++P PFLSKTY+LLEE G+  + ++D +      E        + ++SWN EGNG
Sbjct: 36  QSPMTKSPTPFLSKTYNLLEEGGAGIIMSSDHDRSIINGEYYAGSYKRSSVISWNEEGNG 95

Query: 62  FIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGE 121
           F+VWSPAEFSE+LLPKYFKHNNFSSF+RQLNTYGFKKTS KRWEFKH+KFQ+G K ML E
Sbjct: 96  FVVWSPAEFSELLLPKYFKHNNFSSFVRQLNTYGFKKTSSKRWEFKHDKFQKGCKHMLVE 155

Query: 122 ILRKKCEPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKA 181
           I RKKCEPSVFPAF ++S   S   A   +  + LQLMEENKNLRR K+ELQ +I  FKA
Sbjct: 156 ITRKKCEPSVFPAFRKASDRDS---AAATEENNRLQLMEENKNLRREKVELQTQIAHFKA 215

Query: 182 LHIRLLDCL 184
           L ++LLDCL
Sbjct: 216 LEVKLLDCL 221

[Close]

BLAST of CmaCh07G002470.1 vs. TAIR10
Match: AT5G62020.1 (AT5G62020.1 heat shock transcription factor B2A)

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 71/202 (35.15%), Postives = 110/202 (54.46%), Query Frame = 1


Query: 3   SSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAE 62
           SS+   P PFL+KT++L+E++                   + ++SWN +G+ FIVW+P +
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSI-----------------DDVISWNEDGSSFIVWNPTD 74

Query: 63  FSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEP 122
           F++ LLPK+FKHNNFSSF+RQLNTYGFKK    RWEF ++ F+RG K++L EI R+K   
Sbjct: 75  FAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITT 134

Query: 123 SVFPAFLRSSHEGSETMAVGQKN-----------------------------GDHLQLME 176
           +       SS + ++TM V   N                             G  ++L+E
Sbjct: 135 THQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLE 194

[Close]

BLAST of CmaCh07G002470.1 vs. TAIR10
Match: AT5G16820.1 (AT5G16820.1 heat shock factor 3)

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-27
Identity = 70/163 (42.94%), Postives = 96/163 (58.90%), Query Frame = 1


Query: 11  PFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPK 70
           PFLSKTYD++++  +                 N +VSW++  N F+VWS  EFS+VLLPK
Sbjct: 27  PFLSKTYDMVDDPLT-----------------NEVVSWSSGNNSFVVWSAPEFSKVLLPK 86

Query: 71  YFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAFLR 130
           YFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG KQ+L  I+R+K  PS      +
Sbjct: 87  YFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRK--PSHVQQNQQ 146

Query: 131 SSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFK 174
            +   S ++    + G    + EE + L+R K  L   +VR +
Sbjct: 147 QTQVQSSSVGACVEVG-KFGIEEEVERLKRDKNVLMQELVRLR 169

[Close]

BLAST of CmaCh07G002470.1 vs. TAIR10
Match: AT1G32330.1 (AT1G32330.1 heat shock transcription factor A1D)

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-27
Identity = 70/166 (42.17%), Postives = 89/166 (53.61%), Query Frame = 1


Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++++  +                 + IVSW+A  N FIVW P EF+  LL
Sbjct: 35  PPPFLSKTYDMVDDHNT-----------------DSIVSWSANNNSFIVWKPPEFARDLL 94

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAF 128
           PK FKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L  I R+K         
Sbjct: 95  PKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQGH 154

Query: 129 LRSSHEGSETMAVGQ-KNGDHLQLMEENKNLRRRKLELQARIVRFK 174
            RS H   +  +V          L EE + L+R K  L   +VR +
Sbjct: 155 QRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLR 183

[Close]

BLAST of CmaCh07G002470.1 vs. TAIR10
Match: AT4G17750.1 (AT4G17750.1 heat shock factor 1)

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-27
Identity = 75/174 (43.10%), Postives = 97/174 (55.75%), Query Frame = 1


Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++E+  +                 + IVSW+   N FIVW P EFS  LL
Sbjct: 50  PPPFLSKTYDMVEDPAT-----------------DAIVSWSPTNNSFIVWDPPEFSRDLL 109

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAF 128
           PKYFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L +I R+K   SV    
Sbjct: 110 PKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRK---SVQGHG 169

Query: 129 LRSSHEGSETMAVGQKNGDHLQ---------LMEENKNLRRRKLELQARIVRFK 174
             SS+  S+ ++ GQ +   L          L EE + L+R K  L   +V+ +
Sbjct: 170 SSSSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLR 203

[Close]

BLAST of CmaCh07G002470.1 vs. TAIR10
Match: AT4G13980.1 (AT4G13980.1 winged-helix DNA-binding transcription factor family protein)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-26
Identity = 67/165 (40.61%), Postives = 95/165 (57.58%), Query Frame = 1


Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           PAPFL KTY++++++ +                 ++IVSW+A  N FIVW+ AEFS +LL
Sbjct: 21  PAPFLVKTYEMVDDSST-----------------DQIVSWSANNNSFIVWNHAEFSRLLL 80

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCEPSVFPAF 128
           P YFKHNNFSSFIRQLNTYGF+K   +RWEF ++ F +  K +L  I R+K      P  
Sbjct: 81  PTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK------PIH 140

Query: 129 LRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFK 174
             S    S T      + +   L E+   L R K  ++A++++FK
Sbjct: 141 SHSHPPASST------DQERAVLQEQMDKLSREKAAIEAKLLKFK 156

[Close]

BLAST of CmaCh07G002470.1 vs. NCBI nr
Match: gi|659078703|ref|XP_008439862.1| (PREDICTED: heat stress transcription factor B-2a-like [Cucumis melo])

HSP 1 Score: 322.4 bits (825), Expect = 5.3e-85
Identity = 162/198 (81.82%), Postives = 176/198 (88.89%), Query Frame = 1


Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE---------NRIVSWNAE 60
           MQSS+TRNPAPFLSKTYDLLEEAGSFH K ADQE+E+E+EEE         NRIVSWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 61  GNGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQM 120
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG + M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 121 LGEILRKKCEPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVR 180
           L EI+RKKCEPSVFPAFLRSSHEG  TMAV Q+NGDHL LMEEN NLRR+KLELQA+I +
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 181 FKALHIRLLDCLDQCIKK 190
           FKALHIRLLDCL QCI+K
Sbjct: 181 FKALHIRLLDCLGQCIEK 198

[Close]

BLAST of CmaCh07G002470.1 vs. NCBI nr
Match: gi|778720797|ref|XP_011658207.1| (PREDICTED: heat stress transcription factor B-2a-like [Cucumis sativus])

HSP 1 Score: 316.2 bits (809), Expect = 3.8e-83
Identity = 159/197 (80.71%), Postives = 174/197 (88.32%), Query Frame = 1


Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE--------NRIVSWNAEG 60
           MQSS+TRNPAPFLSKTYDLLEEAGSFH + ADQ++EE+EEEE        NR VSWNAEG
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFEEADQDQEEDEEEEEEEEKNGGNRTVSWNAEG 60

Query: 61  NGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQML 120
           +GFIVWSPA+FSE LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG + ML
Sbjct: 61  SGFIVWSPADFSEHLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHML 120

Query: 121 GEILRKKCEPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRF 180
            EI+RKKCEPSVFPAFLRSSHEG  TMAV Q+NGDHL LMEEN NLRR+KLELQA+I +F
Sbjct: 121 VEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQF 180

Query: 181 KALHIRLLDCLDQCIKK 190
           KALHIRLLDCL +CI+K
Sbjct: 181 KALHIRLLDCLGRCIEK 197

[Close]

BLAST of CmaCh07G002470.1 vs. NCBI nr
Match: gi|645237175|ref|XP_008225084.1| (PREDICTED: heat stress transcription factor B-2a-like [Prunus mume])

HSP 1 Score: 232.6 bits (592), Expect = 5.5e-58
Identity = 122/187 (65.24%), Postives = 147/187 (78.61%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPA 61
           QS +TR+PAPFLSKTYDLLE+  +           EE +   +IVSWNAEG+GFIVWSPA
Sbjct: 9   QSPRTRSPAPFLSKTYDLLEKGAA-----------EEGDSGKKIVSWNAEGSGFIVWSPA 68

Query: 62  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCE 121
           EFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS K+WEFKHEKFQ+G + ML EI RKKCE
Sbjct: 69  EFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSPKQWEFKHEKFQKGCRHMLVEITRKKCE 128

Query: 122 PSVFPAFLRSSHE---GSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIR 181
           PS FP +L++S E    S T+A  ++N + L LMEENKNLR++KLELQ ++ +FKAL ++
Sbjct: 129 PSAFPVYLKASEESGSSSTTVAAAEEN-NRLLLMEENKNLRKQKLELQMQLSQFKALEMK 183

Query: 182 LLDCLDQ 186
           LLDCL Q
Sbjct: 189 LLDCLAQ 183

[Close]

BLAST of CmaCh07G002470.1 vs. NCBI nr
Match: gi|356495198|ref|XP_003516466.1| (PREDICTED: heat stress transcription factor B-2a-like [Glycine max])

HSP 1 Score: 232.3 bits (591), Expect = 7.2e-58
Identity = 118/184 (64.13%), Postives = 139/184 (75.54%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPA 61
           QS + R PAPFL KTYDLLEE G            E  E+  +IVSWNAEG GF+VWSP+
Sbjct: 25  QSPRQRCPAPFLLKTYDLLEELG------------ENIEDSTKIVSWNAEGTGFVVWSPS 84

Query: 62  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCE 121
           EFSE+ LP+YFKHNNFSSFIRQLNTYGFKK S KRWEFKHEKFQRG + MLGEI RKKCE
Sbjct: 85  EFSELTLPRYFKHNNFSSFIRQLNTYGFKKISSKRWEFKHEKFQRGCRHMLGEITRKKCE 144

Query: 122 PSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRLLD 181
           PSVFPA+L+SS E +   +  ++N DH  LMEENKNL++ +LELQ +I   KAL ++LL+
Sbjct: 145 PSVFPAYLKSSSEENNATSSTEENNDHQLLMEENKNLKKERLELQVQIDECKALEMKLLE 196

Query: 182 CLDQ 186
           CL Q
Sbjct: 205 CLSQ 196

[Close]

BLAST of CmaCh07G002470.1 vs. NCBI nr
Match: gi|595874379|ref|XP_007212104.1| (hypothetical protein PRUPE_ppa011804mg [Prunus persica])

HSP 1 Score: 230.7 bits (587), Expect = 2.1e-57
Identity = 119/186 (63.98%), Postives = 143/186 (76.88%), Query Frame = 1


Query: 2   QSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPA 61
           QS +TR+PAPFLSKTYDLLEE  +           EE +   +IVSWNAEG+GF+VWSPA
Sbjct: 9   QSPRTRSPAPFLSKTYDLLEEGAA-----------EEGDSGKKIVSWNAEGSGFVVWSPA 68

Query: 62  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKCE 121
           EFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS ++WEFKHE+FQ+G + ML EI RKKCE
Sbjct: 69  EFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSPRQWEFKHERFQKGCRHMLVEITRKKCE 128

Query: 122 PSVFPAFLRSSHE--GSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRL 181
           PS FP +L++S E   S T     +  + L LMEENKNLR++KLELQ +I +FKAL ++L
Sbjct: 129 PSAFPVYLKASEESGSSNTAVAAAEENNCLLLMEENKNLRKQKLELQMQISQFKALEMKL 183

Query: 182 LDCLDQ 186
           LDCL Q
Sbjct: 189 LDCLAQ 183

The following BLAST results are available for this feature:

BLAST of CmaCh07G002470.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HFB2A_ARATH	2.0e-28	35.15	Heat stress transcription factor B-2a OS=Arabidopsis thaliana GN=HSFB2A PE=2 SV=...	[more]
HFA1B_ARATH	1.9e-26	42.94	Heat stress transcription factor A-1b OS=Arabidopsis thaliana GN=HSFA1B PE=2 SV=...	[more]
HFA1A_ARATH	1.9e-26	43.10	Heat stress transcription factor A-1a OS=Arabidopsis thaliana GN=HSFA1A PE=1 SV=...	[more]
HFA1D_ARATH	1.9e-26	42.17	Heat stress transcription factor A-1d OS=Arabidopsis thaliana GN=HSFA1D PE=1 SV=...	[more]
HSFA5_ARATH	2.1e-25	40.61	Heat stress transcription factor A-5 OS=Arabidopsis thaliana GN=HSFA5 PE=2 SV=1	[more]

BLAST of CmaCh07G002470.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KI26_CUCSA	2.6e-83	80.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G517310 PE=3 SV=1	[more]
I1J7X9_SOYBN	5.0e-58	64.13	Uncharacterized protein OS=Glycine max GN=GLYMA_01G143500 PE=3 SV=1	[more]
A0A0B2S611_GLYSO	5.0e-58	64.13	Heat stress transcription factor B-2a OS=Glycine soja GN=glysoja_028764 PE=3 SV=...	[more]
M5WVD4_PRUPE	1.5e-57	63.98	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011804mg PE=3 SV=1	[more]
W9RZ03_9ROSA	4.2e-57	62.43	Heat stress transcription factor B-2a OS=Morus notabilis GN=L484_014528 PE=3 SV=...	[more]

BLAST of CmaCh07G002470.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62020.1	1.1e-29	35.15	heat shock transcription factor B2A	[more]
AT5G16820.1	1.1e-27	42.94	heat shock factor 3	[more]
AT1G32330.1	1.1e-27	42.17	heat shock transcription factor A1D	[more]
AT4G17750.1	1.1e-27	43.10	heat shock factor 1	[more]
AT4G13980.1	1.2e-26	40.61	winged-helix DNA-binding transcription factor family protein	[more]

BLAST of CmaCh07G002470.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659078703\|ref\|XP_008439862.1\|	5.3e-85	81.82	PREDICTED: heat stress transcription factor B-2a-like [Cucumis melo]	[more]
gi\|778720797\|ref\|XP_011658207.1\|	3.8e-83	80.71	PREDICTED: heat stress transcription factor B-2a-like [Cucumis sativus]	[more]
gi\|645237175\|ref\|XP_008225084.1\|	5.5e-58	65.24	PREDICTED: heat stress transcription factor B-2a-like [Prunus mume]	[more]
gi\|356495198\|ref\|XP_003516466.1\|	7.2e-58	64.13	PREDICTED: heat stress transcription factor B-2a-like [Glycine max]	[more]
gi\|595874379\|ref\|XP_007212104.1\|	2.1e-57	63.98	hypothetical protein PRUPE_ppa011804mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000232	HSF_DNA-bd
IPR011991	Winged helix-turn-helix DNA-binding domain
IPR027725	HSF_fam

Vocabulary: **Molecular Function**
Term	Definition
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0009408	response to heat
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh07G002470	CmaCh07G002470	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh07G002470.1	CmaCh07G002470.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh07G002470.1.exon.1	CmaCh07G002470.1.exon.1	exon
CmaCh07G002470.1.exon.2	CmaCh07G002470.1.exon.2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh07G002470.1.five_prime_UTR.1	CmaCh07G002470.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh07G002470.1.CDS.1	CmaCh07G002470.1.CDS.1	CDS
CmaCh07G002470.1.CDS.2	CmaCh07G002470.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh07G002470.1.three_prime_UTR.1	CmaCh07G002470.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 67..79 score: 9.8E-12coord: 80..92 score: 9.8E-12coord: 29..52 score: 9.8
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 12..118 score: 8.4
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 8..118 score: 1.8
IPR011991	Winged helix-turn-helix DNA-binding domain	GENE3D	G3DSA:1.10.10.10		coord: 3..112 score: 3.2
IPR011991	Winged helix-turn-helix DNA-binding domain	unknown	SSF46785	"Winged helix" DNA-binding domain	coord: 39..118 score: 3.67
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 42..187 score: 1.0E-95coord: 2..24 score: 1.0
None	No IPR available	unknown	Coil	Coil	coord: 148..175 score: -coord: 31..51 scor
None	No IPR available	PANTHER	PTHR10015:SF155	SUBFAMILY NOT NAMED	coord: 42..187 score: 1.0E-95coord: 2..24 score: 1.0