CmaCh01G001960.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh01G001960.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Chaperone protein dnaJ 8
Location	Cma_Chr01 : 893316 .. 894693 (+)
Sequence length	962

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTAAAATTAACTCACTGGATAAGAACCGACGATCTCGCCTCTCCGCACCCTCCGTATCTAAAATCCTCTTCCTCTTCCTTTCTATTTAATTCGCTCTTCCCCTTTCTCGATCCCTCCAATTATATCCATCTTTGCATCGCTTTTGCCTCCGATTATCCTGGTTTTGAAGCTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTTAATGGCGTCTGTTTCTACTGCTGCTGGATTCATTGCCCGGACTGGTTCTTCGTCTTGGATTCGCCAACGAAATCGGGCGAACAAGCTTCATCGGAGTTCGAGGAATCAAGTTAGGGCTTCTTATTCTTCTTATTCCTCTTCTTCTATGGCTGATCCTTACCGGACTCTTCGGATTCAACCTGGCGCCTCTGAATCTGAGGTCAAAAAAGCCTTCCGTCAACTCGCTCTTCAGGTTCGATTTCTCTCTCGCTCTCTCTGTCTCTCTATTCTTTCTCGATTTGTGGACTTCCTCATCTGATTCCTTGAATTTTATCGGTTTTTCAGTATCATCCGGATGTATGCAAAGGAAGTAATTGTGGAGTGCAATTTCATCAAATCAATGAAGCCTACGATGTACGTGATCCAAGCCCTAACTAATTAATTATCCTCTTCCATTATTGCATATATTATATTAATAATCGGATCAATTATTTGAATCGTTTTCTTAAAAATAAAAATAAAAATAAAAATAATTGAGCAGCAAACGCGGTAACTAGTATTGATTATGATTTCTTGTCGGAGTATTTTTAATTCGAGGCGTCAATCGAGTGTTTGGATCAGATCCATATCGGTTCATCTAATATTGAAATCGGTATACTTATCTGTTGCATATATCATTTTCATGTTAGTTCAATCACAATACAAAACTAATGTCATATATATTTGCGTCTGAAACAGATTGTGATGAACAATTTAAGAGGAATATCAATGCCAATCGAGACGTACGACGAAGGGGGTGACGAACCGATGAGGGGAATGGGGGATCCGGATTGGGATTTGTGGGAGGAATGGATGGGATGGGAAGGCGCAGGGATTCGAGATTATTCATCGCATATAAACCCGTACATTTAAATGAAATTGGTGTGAAGGAACATAATTTGTTCATAGAACTGAGCCCTAATCTATCAACATCATCCAATGGTGGTGGTTGCTTGTGCATATAATTTGGATAGGGAGAGATTGAAGAATGGCTCTGTCTTGATGTATCCACTTTTGTACATAAGGTCAAGACAATGGCTTTGGGTTTTAATAATAATGTGAGAAGGCTTTCAAGATGCCCTTTCGAATTTGTGGGCCCTTTGTGTCTTATCTTTTGTCCTATTTAAGATATTAAATGATTGTGAAGTTGGTAAGCTC

mRNA sequence

TCTAAAATTAACTCACTGGATAAGAACCGACGATCTCGCCTCTCCGCACCCTCCGTATCTAAAATCCTCTTCCTCTTCCTTTCTATTTAATTCGCTCTTCCCCTTTCTCGATCCCTCCAATTATATCCATCTTTGCATCGCTTTTGCCTCCGATTATCCTGGTTTTGAAGCTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTTAATGGCGTCTGTTTCTACTGCTGCTGGATTCATTGCCCGGACTGGTTCTTCGTCTTGGATTCGCCAACGAAATCGGGCGAACAAGCTTCATCGGAGTTCGAGGAATCAAGTTAGGGCTTCTTATTCTTCTTATTCCTCTTCTTCTATGGCTGATCCTTACCGGACTCTTCGGATTCAACCTGGCGCCTCTGAATCTGAGGTCAAAAAAGCCTTCCGTCAACTCGCTCTTCAGTATCATCCGGATGTATGCAAAGGAAGTAATTGTGGAGTGCAATTTCATCAAATCAATGAAGCCTACGATATTGTGATGAACAATTTAAGAGGAATATCAATGCCAATCGAGACGTACGACGAAGGGGGTGACGAACCGATGAGGGGAATGGGGGATCCGGATTGGGATTTGTGGGAGGAATGGATGGGATGGGAAGGCGCAGGGATTCGAGATTATTCATCGCATATAAACCCGTACATTTAAATGAAATTGGTGTGAAGGAACATAATTTGTTCATAGAACTGAGCCCTAATCTATCAACATCATCCAATGGTGGTGGTTGCTTGTGCATATAATTTGGATAGGGAGAGATTGAAGAATGGCTCTGTCTTGATGTATCCACTTTTGTACATAAGGTCAAGACAATGGCTTTGGGTTTTAATAATAATGTGAGAAGGCTTTCAAGATGCCCTTTCGAATTTGTGGGCCCTTTGTGTCTTATCTTTTGTCCTATTTAAGATATTAAATGATTGTGAAGTTGGTAAGCTC

Coding sequence (CDS)

ATGGCGTCTGTTTCTACTGCTGCTGGATTCATTGCCCGGACTGGTTCTTCGTCTTGGATTCGCCAACGAAATCGGGCGAACAAGCTTCATCGGAGTTCGAGGAATCAAGTTAGGGCTTCTTATTCTTCTTATTCCTCTTCTTCTATGGCTGATCCTTACCGGACTCTTCGGATTCAACCTGGCGCCTCTGAATCTGAGGTCAAAAAAGCCTTCCGTCAACTCGCTCTTCAGTATCATCCGGATGTATGCAAAGGAAGTAATTGTGGAGTGCAATTTCATCAAATCAATGAAGCCTACGATATTGTGATGAACAATTTAAGAGGAATATCAATGCCAATCGAGACGTACGACGAAGGGGGTGACGAACCGATGAGGGGAATGGGGGATCCGGATTGGGATTTGTGGGAGGAATGGATGGGATGGGAAGGCGCAGGGATTCGAGATTATTCATCGCATATAAACCCGTACATTTAA

Protein sequence

MASVSTAAGFIARTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPIETYDEGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI

Homology

[Close]

BLAST of CmaCh01G001960.1 vs. Swiss-Prot
Match: DNAJ8_ARATH (Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana GN=ATJ8 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 4.8e-39
Identity = 85/149 (57.05%), Postives = 104/149 (69.80%), Query Frame = 1


Query: 14  TGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGASESEVKKAFRQ 73
           + SSS+  + +R       +R++V  S    SSSS+ DPY+TL+I+P +SE EVKKAFRQ
Sbjct: 22  SSSSSFRLKNSRRKNTKMLNRSKVVCS----SSSSVMDPYKTLKIRPDSSEYEVKKAFRQ 81

Query: 74  LALQYHPDVCKGSNCGVQFHQINEAYDIVM----NNLRGIS--MPIETYDEGGDEPMRGM 133
           LA +YHPDVC+GSNCGVQF  INEAYDIV+    N + G     P + YDEG    + GM
Sbjct: 82  LAKKYHPDVCRGSNCGVQFQTINEAYDIVLKQIKNQMEGTEEFEPFDVYDEG----LNGM 141

Query: 134 GDPDWDLWEEWMGWEGAGIRDYSSHINPY 157
            DPD D WEEWMGWEGAG RDYSSH+NPY
Sbjct: 142 NDPDCDTWEEWMGWEGAGTRDYSSHVNPY 162

[Close]

BLAST of CmaCh01G001960.1 vs. Swiss-Prot
Match: DNJA1_RAT (DnaJ homolog subfamily A member 1 OS=Rattus norvegicus GN=Dnaja1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.0e-09
Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1


Query: 53  YRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP 112
           Y  L ++P A++ E+KKA+R+LAL+YHPD  K  N G +F QI++AY+++ +     S  
Sbjct: 8   YDVLGVKPNATQEELKKAYRKLALKYHPD--KNPNEGEKFKQISQAYEVLAD-----SKK 67

Query: 113 IETYDEGGDEPMR------GMGDPDWDLWEEWMGWEG 144
            E YD+GG++ ++      G G P  D+++ + G  G
Sbjct: 68  RELYDKGGEQAIKEGGAGGGFGSP-MDIFDMFFGGGG 96

[Close]

BLAST of CmaCh01G001960.1 vs. Swiss-Prot
Match: DNJA1_MOUSE (DnaJ homolog subfamily A member 1 OS=Mus musculus GN=Dnaja1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.0e-09
Identity = 36/97 (37.11%), Postives = 60/97 (61.86%), Query Frame = 1


Query: 53  YRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP 112
           Y  L ++P A++ E+KKA+R+LAL+YHPD  K  N G +F QI++AY+++ +     S  
Sbjct: 8   YDVLGVKPNATQEELKKAYRKLALKYHPD--KNPNEGEKFKQISQAYEVLAD-----SKK 67

Query: 113 IETYDEGGDEPMR------GMGDPDWDLWEEWMGWEG 144
            E YD+GG++ ++      G G P  D+++ + G  G
Sbjct: 68  RELYDKGGEQAIKEGGAGGGFGSP-MDIFDMFFGGGG 96

[Close]

BLAST of CmaCh01G001960.1 vs. Swiss-Prot
Match: DNAJ_BIFLO (Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) GN=dnaJ PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.1e-09
Identity = 39/104 (37.50%), Postives = 55/104 (52.88%), Query Frame = 1


Query: 49  MADPYRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRG 108
           MAD Y TL ++ GAS+ E+KKA+R+L+ +YHPD+  G     +F ++N AYD++ N  + 
Sbjct: 1   MADYYETLGVERGASDDEIKKAYRKLSRKYHPDIA-GPEFEDKFKEVNNAYDVLSNPDKR 60

Query: 109 ISMPIETYDEGGD--EPMRGMGDPDWDLWEEWMGWEGAGIRDYS 151
                  YD G D   P  G G           G+ GAG  D S
Sbjct: 61  -----RMYDSGVDPNNPNAGAG-----------GFSGAGFGDMS 87

[Close]

BLAST of CmaCh01G001960.1 vs. Swiss-Prot
Match: DNJA1_HUMAN (DnaJ homolog subfamily A member 1 OS=Homo sapiens GN=DNAJA1 PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 6.7e-09
Identity = 35/97 (36.08%), Postives = 60/97 (61.86%), Query Frame = 1


Query: 53  YRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP 112
           Y  L ++P A++ E+KKA+R+LAL+YHPD  K  N G +F QI++AY+++ +     +  
Sbjct: 8   YDVLGVKPNATQEELKKAYRKLALKYHPD--KNPNEGEKFKQISQAYEVLSD-----AKK 67

Query: 113 IETYDEGGDEPMR------GMGDPDWDLWEEWMGWEG 144
            E YD+GG++ ++      G G P  D+++ + G  G
Sbjct: 68  RELYDKGGEQAIKEGGAGGGFGSP-MDIFDMFFGGGG 96

[Close]

BLAST of CmaCh01G001960.1 vs. TrEMBL
Match: Q2L989_CAMSI (Putative DnaJ protein OS=Camellia sinensis PE=2 SV=2)

HSP 1 Score: 216.1 bits (549), Expect = 3.1e-53
Identity = 111/161 (68.94%), Postives = 129/161 (80.12%), Query Frame = 1


Query: 4   VSTAAGFIAR-TGSSSWIRQRNRANKLHRSSRNQVRASYSSY-SSSSMADPYRTLRIQPG 63
           +++ AG I R  GSSSWI+ +N   K      ++VR   +SY +SS++ DPY+TLRIQPG
Sbjct: 1   MASVAGMIGRGCGSSSWIQLKNSKKKKAMRKNDRVRVCTASYSASSAVTDPYKTLRIQPG 60

Query: 64  ASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGIS-MP----IETY 123
           ASESEVKKAFRQLALQYHPDVC+G+NCGVQFH INEAYDIVM+NLRG S +P     E+Y
Sbjct: 61  ASESEVKKAFRQLALQYHPDVCRGNNCGVQFHHINEAYDIVMSNLRGESTLPEMEMYESY 120

Query: 124 DEGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
           D   DEPMRGM DPDWD+WEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 D--ADEPMRGMNDPDWDMWEEWMGWEGAGIRDYSSHINPYI 159

[Close]

BLAST of CmaCh01G001960.1 vs. TrEMBL
Match: A0A061GAB8_THECC (Chaperone DnaJ-domain superfamily protein OS=Theobroma cacao GN=TCM_027478 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.0e-52
Identity = 108/157 (68.79%), Postives = 123/157 (78.34%), Query Frame = 1


Query: 4   VSTAAGFIARTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGAS 63
           +++A G I R GSSSW++ + +  K    + N+VR   S+   SS+ DPY+TLRIQ GAS
Sbjct: 1   MASAVGMIGRNGSSSWMQVKGKGKK---KTVNRVRVRCSA---SSVMDPYKTLRIQRGAS 60

Query: 64  ESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRG---ISMPIETYDEGG 123
           ESEVKKAFRQLALQYHPDVC+GSNCGVQF  INEAYDIVM+N RG    S   E YDEG 
Sbjct: 61  ESEVKKAFRQLALQYHPDVCRGSNCGVQFQTINEAYDIVMSNFRGESNESRMYEAYDEGI 120

Query: 124 DEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
           DEPMRGM DPDWD+WEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 DEPMRGMDDPDWDMWEEWMGWEGAGIRDYSSHINPYI 151

[Close]

BLAST of CmaCh01G001960.1 vs. TrEMBL
Match: M5WS61_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012860mg PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.8e-52
Identity = 106/156 (67.95%), Postives = 122/156 (78.21%), Query Frame = 1


Query: 5   STAAGFIARTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGASE 64
           + AAG I   GSS W   +++  K    ++  V   YSS     + DPY+TL+IQPGASE
Sbjct: 3   AVAAGVIGGNGSS-WNLFKDKPRKKKTVNKVMVSCVYSS-----VMDPYQTLKIQPGASE 62

Query: 65  SEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP---IETYDEGGD 124
           SEV+KAFRQLAL+YHPDVC+G+NCGVQFHQINEAYDIVM+NLRG S P    ETYDEG D
Sbjct: 63  SEVRKAFRQLALKYHPDVCRGNNCGVQFHQINEAYDIVMSNLRGESTPSPMYETYDEGVD 122

Query: 125 EPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
           EPMRGM DPDWD+WEEWMGWEGAGIRDY+SHINPYI
Sbjct: 123 EPMRGMNDPDWDMWEEWMGWEGAGIRDYTSHINPYI 152

[Close]

BLAST of CmaCh01G001960.1 vs. TrEMBL
Match: A0A0B2P4K3_GLYSO (Chaperone protein dnaJ 8, chloroplastic OS=Glycine soja GN=glysoja_032687 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.8e-52
Identity = 105/158 (66.46%), Postives = 124/158 (78.48%), Query Frame = 1


Query: 5   STAAGFIARTGSS-SWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGAS 64
           +  AG +   GSS SW+R + +  K  + +++++  SYSS    S+ DPY+TLRIQ GAS
Sbjct: 3   AATAGVVGGNGSSASWMRFKGKERKQTKMNKSRISCSYSS----SVMDPYKTLRIQRGAS 62

Query: 65  ESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPIET----YDEG 124
           ESEV+KAFRQLALQYHPDVC+GSNCGVQFH+INEAYDIVM NLRG S   E+    YD G
Sbjct: 63  ESEVRKAFRQLALQYHPDVCRGSNCGVQFHEINEAYDIVMANLRGESNATESYEAYYDAG 122

Query: 125 GDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
            DEP+RGM DPDWD+WEEWMGWEGAGIRDYSSHINPYI
Sbjct: 123 IDEPLRGMNDPDWDMWEEWMGWEGAGIRDYSSHINPYI 156

[Close]

BLAST of CmaCh01G001960.1 vs. TrEMBL
Match: M1B4C5_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400014210 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 7.6e-52
Identity = 106/163 (65.03%), Postives = 127/163 (77.91%), Query Frame = 1


Query: 4   VSTAAGFIARTG-----SSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRI 63
           ++TA G +   G     S+S  R RN A K  R+ +N  R S   YSSS++ADPY+TL+I
Sbjct: 1   MATAMGMMGSIGGCGAASASLFRLRNSAKKKTRNDKNGFRVS-CVYSSSAVADPYKTLKI 60

Query: 64  QPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP----IE 123
           QPGASESEV+KAFRQLAL+YHPDVC+G+NCGVQFHQINEAYD+VM+NLRG +      IE
Sbjct: 61  QPGASESEVRKAFRQLALKYHPDVCRGNNCGVQFHQINEAYDVVMSNLRGETRAELEMIE 120

Query: 124 TYDEGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
            YD+  DE MRGM +PDWDLWEEWMGWEGAGIRDY+SH+NPYI
Sbjct: 121 EYDDSNDESMRGMYEPDWDLWEEWMGWEGAGIRDYTSHVNPYI 162

[Close]

BLAST of CmaCh01G001960.1 vs. TAIR10
Match: AT1G80920.1 (AT1G80920.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 162.2 bits (409), Expect = 2.7e-40
Identity = 85/149 (57.05%), Postives = 104/149 (69.80%), Query Frame = 1


Query: 14  TGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGASESEVKKAFRQ 73
           + SSS+  + +R       +R++V  S    SSSS+ DPY+TL+I+P +SE EVKKAFRQ
Sbjct: 22  SSSSSFRLKNSRRKNTKMLNRSKVVCS----SSSSVMDPYKTLKIRPDSSEYEVKKAFRQ 81

Query: 74  LALQYHPDVCKGSNCGVQFHQINEAYDIVM----NNLRGIS--MPIETYDEGGDEPMRGM 133
           LA +YHPDVC+GSNCGVQF  INEAYDIV+    N + G     P + YDEG    + GM
Sbjct: 82  LAKKYHPDVCRGSNCGVQFQTINEAYDIVLKQIKNQMEGTEEFEPFDVYDEG----LNGM 141

Query: 134 GDPDWDLWEEWMGWEGAGIRDYSSHINPY 157
            DPD D WEEWMGWEGAG RDYSSH+NPY
Sbjct: 142 NDPDCDTWEEWMGWEGAGTRDYSSHVNPY 162

[Close]

BLAST of CmaCh01G001960.1 vs. TAIR10
Match: AT1G72070.1 (AT1G72070.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-11
Identity = 36/91 (39.56%), Postives = 52/91 (57.14%), Query Frame = 1


Query: 53  YRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP 112
           Y  L + P AS++EVK+AF++LAL+YHPDV KG +    F +I  AY+ +M   +     
Sbjct: 41  YTVLGLTPLASQTEVKRAFKRLALKYHPDVHKGQD--KDFKEIKSAYECLMQKFKKEEEE 100

Query: 113 IETYDEGGDEPMRGMGDPDWDLWEEWMGWEG 144
           +E  + G           + D WEEWMG+EG
Sbjct: 101 MEITEMG-----------EIDEWEEWMGFEG 118

[Close]

BLAST of CmaCh01G001960.1 vs. TAIR10
Match: AT5G59610.1 (AT5G59610.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 2.4e-09
Identity = 37/115 (32.17%), Postives = 61/115 (53.04%), Query Frame = 1


Query: 44  YSSSSMADPYRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVM 103
           + + + + PY  L + P A+  ++K+A+R+LAL+YHPDV K +N   +F +I  AY  ++
Sbjct: 66  FVTRARSSPYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEANAQEKFLKIKHAYTTLI 125

Query: 104 NN-----------LRGISMPIETYDEGG---DEPMRGMGDPDWDLWEEWMGWEGA 145
           N+             G S   +T  +G    +E   G+GD   DL EE+  WE +
Sbjct: 126 NSDSRRKYGSDSRATGSSTG-QTSRKGNSQVEEDFYGLGDFFKDLQEEYKNWEAS 179

[Close]

BLAST of CmaCh01G001960.1 vs. TAIR10
Match: AT3G05345.1 (AT3G05345.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 53.9 bits (128), Expect = 1.0e-07
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 1


Query: 48  SMADPYRTLRIQPGASESEVKKAFRQLALQYHPDVCK-GSNCGVQFHQINEAYDIVMNNL 107
           S + PY  L ++P  S SE+K AFR    QYHPDV K GSN  +   +I +AY+++ N  
Sbjct: 41  SSSSPYSILGVEPSCSSSELKAAFRAKVKQYHPDVNKDGSNSDIMIRRIIQAYEMLTNYS 100

[Close]

BLAST of CmaCh01G001960.1 vs. TAIR10
Match: AT3G08970.1 (AT3G08970.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 52.8 bits (125), Expect = 2.3e-07
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1


Query: 51  DPYRTLRIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGIS 110
           DPY+ L +   A + E++KAF + +L+YHPD  K      +F +IN AY+I+ +  +   
Sbjct: 27  DPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKDKGAQEKFAEINNAYEILSDEEKR-- 86

Query: 111 MPIETYDEGGDEPMRGMGDPDWD 134
              + YD  GDE     G P +D
Sbjct: 87  ---KNYDLYGDE----KGQPGFD 100

[Close]

BLAST of CmaCh01G001960.1 vs. NCBI nr
Match: gi|659130374|ref|XP_008465135.1| (PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo])

HSP 1 Score: 249.6 bits (636), Expect = 3.6e-63
Identity = 134/165 (81.21%), Postives = 141/165 (85.45%), Query Frame = 1


Query: 1   MASVSTAAGFIARTGSSS----WIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTL 60
           MASV+TA GFIAR+ SSS     IR  NR  K +R+S NQ+R S S  SSSS+ADPYRTL
Sbjct: 1   MASVATA-GFIARSSSSSSSSSCIRNTNRPKK-NRTSSNQLRVSCS--SSSSVADPYRTL 60

Query: 61  RIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPIET- 120
           RIQPGASESEVKKAFR LALQYHPDVC+GSNCGVQFHQINEAYDIVMNNLRGIS PIET 
Sbjct: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120

Query: 121 ---YDEGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
              YDEG DEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 ETIYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 161

[Close]

BLAST of CmaCh01G001960.1 vs. NCBI nr
Match: gi|145688390|gb|ABC69274.2| (putative DnaJ protein [Camellia sinensis])

HSP 1 Score: 216.1 bits (549), Expect = 4.4e-53
Identity = 111/161 (68.94%), Postives = 129/161 (80.12%), Query Frame = 1


Query: 4   VSTAAGFIAR-TGSSSWIRQRNRANKLHRSSRNQVRASYSSY-SSSSMADPYRTLRIQPG 63
           +++ AG I R  GSSSWI+ +N   K      ++VR   +SY +SS++ DPY+TLRIQPG
Sbjct: 1   MASVAGMIGRGCGSSSWIQLKNSKKKKAMRKNDRVRVCTASYSASSAVTDPYKTLRIQPG 60

Query: 64  ASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGIS-MP----IETY 123
           ASESEVKKAFRQLALQYHPDVC+G+NCGVQFH INEAYDIVM+NLRG S +P     E+Y
Sbjct: 61  ASESEVKKAFRQLALQYHPDVCRGNNCGVQFHHINEAYDIVMSNLRGESTLPEMEMYESY 120

Query: 124 DEGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
           D   DEPMRGM DPDWD+WEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 D--ADEPMRGMNDPDWDMWEEWMGWEGAGIRDYSSHINPYI 159

[Close]

BLAST of CmaCh01G001960.1 vs. NCBI nr
Match: gi|590616291|ref|XP_007023465.1| (Chaperone DnaJ-domain superfamily protein [Theobroma cacao])

HSP 1 Score: 213.4 bits (542), Expect = 2.9e-52
Identity = 108/157 (68.79%), Postives = 123/157 (78.34%), Query Frame = 1


Query: 4   VSTAAGFIARTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGAS 63
           +++A G I R GSSSW++ + +  K    + N+VR   S+   SS+ DPY+TLRIQ GAS
Sbjct: 1   MASAVGMIGRNGSSSWMQVKGKGKK---KTVNRVRVRCSA---SSVMDPYKTLRIQRGAS 60

Query: 64  ESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRG---ISMPIETYDEGG 123
           ESEVKKAFRQLALQYHPDVC+GSNCGVQF  INEAYDIVM+N RG    S   E YDEG 
Sbjct: 61  ESEVKKAFRQLALQYHPDVCRGSNCGVQFQTINEAYDIVMSNFRGESNESRMYEAYDEGI 120

Query: 124 DEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
           DEPMRGM DPDWD+WEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 DEPMRGMDDPDWDMWEEWMGWEGAGIRDYSSHINPYI 151

[Close]

BLAST of CmaCh01G001960.1 vs. NCBI nr
Match: gi|595923676|ref|XP_007214842.1| (hypothetical protein PRUPE_ppa012860mg [Prunus persica])

HSP 1 Score: 211.8 bits (538), Expect = 8.4e-52
Identity = 106/156 (67.95%), Postives = 122/156 (78.21%), Query Frame = 1


Query: 5   STAAGFIARTGSSSWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGASE 64
           + AAG I   GSS W   +++  K    ++  V   YSS     + DPY+TL+IQPGASE
Sbjct: 3   AVAAGVIGGNGSS-WNLFKDKPRKKKTVNKVMVSCVYSS-----VMDPYQTLKIQPGASE 62

Query: 65  SEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMP---IETYDEGGD 124
           SEV+KAFRQLAL+YHPDVC+G+NCGVQFHQINEAYDIVM+NLRG S P    ETYDEG D
Sbjct: 63  SEVRKAFRQLALKYHPDVCRGNNCGVQFHQINEAYDIVMSNLRGESTPSPMYETYDEGVD 122

Query: 125 EPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
           EPMRGM DPDWD+WEEWMGWEGAGIRDY+SHINPYI
Sbjct: 123 EPMRGMNDPDWDMWEEWMGWEGAGIRDYTSHINPYI 152

[Close]

BLAST of CmaCh01G001960.1 vs. NCBI nr
Match: gi|734321530|gb|KHN04191.1| (Chaperone protein dnaJ 8, chloroplastic [Glycine soja])

HSP 1 Score: 211.8 bits (538), Expect = 8.4e-52
Identity = 105/158 (66.46%), Postives = 124/158 (78.48%), Query Frame = 1


Query: 5   STAAGFIARTGSS-SWIRQRNRANKLHRSSRNQVRASYSSYSSSSMADPYRTLRIQPGAS 64
           +  AG +   GSS SW+R + +  K  + +++++  SYSS    S+ DPY+TLRIQ GAS
Sbjct: 3   AATAGVVGGNGSSASWMRFKGKERKQTKMNKSRISCSYSS----SVMDPYKTLRIQRGAS 62

Query: 65  ESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPIET----YDEG 124
           ESEV+KAFRQLALQYHPDVC+GSNCGVQFH+INEAYDIVM NLRG S   E+    YD G
Sbjct: 63  ESEVRKAFRQLALQYHPDVCRGSNCGVQFHEINEAYDIVMANLRGESNATESYEAYYDAG 122

Query: 125 GDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 158
            DEP+RGM DPDWD+WEEWMGWEGAGIRDYSSHINPYI
Sbjct: 123 IDEPLRGMNDPDWDMWEEWMGWEGAGIRDYSSHINPYI 156

The following BLAST results are available for this feature:

BLAST of CmaCh01G001960.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DNAJ8_ARATH	4.8e-39	57.05	Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana GN=ATJ8 PE=2 SV=...	[more]
DNJA1_RAT	3.0e-09	37.11	DnaJ homolog subfamily A member 1 OS=Rattus norvegicus GN=Dnaja1 PE=1 SV=1	[more]
DNJA1_MOUSE	3.0e-09	37.11	DnaJ homolog subfamily A member 1 OS=Mus musculus GN=Dnaja1 PE=1 SV=1	[more]
DNAJ_BIFLO	5.1e-09	37.50	Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) GN=dnaJ PE=3 ...	[more]
DNJA1_HUMAN	6.7e-09	36.08	DnaJ homolog subfamily A member 1 OS=Homo sapiens GN=DNAJA1 PE=1 SV=2	[more]

BLAST of CmaCh01G001960.1 vs. TrEMBL


Match Name	E-value	Identity	Description
Q2L989_CAMSI	3.1e-53	68.94	Putative DnaJ protein OS=Camellia sinensis PE=2 SV=2	[more]
A0A061GAB8_THECC	2.0e-52	68.79	Chaperone DnaJ-domain superfamily protein OS=Theobroma cacao GN=TCM_027478 PE=4 ...	[more]
M5WS61_PRUPE	5.8e-52	67.95	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012860mg PE=4 SV=1	[more]
A0A0B2P4K3_GLYSO	5.8e-52	66.46	Chaperone protein dnaJ 8, chloroplastic OS=Glycine soja GN=glysoja_032687 PE=4 S...	[more]
M1B4C5_SOLTU	7.6e-52	65.03	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400014210 PE=4 SV=1	[more]

BLAST of CmaCh01G001960.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G80920.1	2.7e-40	57.05	Chaperone DnaJ-domain superfamily protein	[more]
AT1G72070.1	3.4e-11	39.56	Chaperone DnaJ-domain superfamily protein	[more]
AT5G59610.1	2.4e-09	32.17	Chaperone DnaJ-domain superfamily protein	[more]
AT3G05345.1	1.0e-07	44.26	Chaperone DnaJ-domain superfamily protein	[more]
AT3G08970.1	2.3e-07	34.94	DNAJ heat shock N-terminal domain-containing protein	[more]

BLAST of CmaCh01G001960.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659130374\|ref\|XP_008465135.1\|	3.6e-63	81.21	PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo]	[more]
gi\|145688390\|gb\|ABC69274.2\|	4.4e-53	68.94	putative DnaJ protein [Camellia sinensis]	[more]
gi\|590616291\|ref\|XP_007023465.1\|	2.9e-52	68.79	Chaperone DnaJ-domain superfamily protein [Theobroma cacao]	[more]
gi\|595923676\|ref\|XP_007214842.1\|	8.4e-52	67.95	hypothetical protein PRUPE_ppa012860mg [Prunus persica]	[more]
gi\|734321530\|gb\|KHN04191.1\|	8.4e-52	66.46	Chaperone protein dnaJ 8, chloroplastic [Glycine soja]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001623	DnaJ_domain

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh01G001960	CmaCh01G001960	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh01G001960.1	CmaCh01G001960.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh01G001960.1.exon.1	CmaCh01G001960.1.exon.1	exon
CmaCh01G001960.1.exon.2	CmaCh01G001960.1.exon.2	exon
CmaCh01G001960.1.exon.3	CmaCh01G001960.1.exon.3	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh01G001960.1.five_prime_UTR.1	CmaCh01G001960.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh01G001960.1.CDS.1	CmaCh01G001960.1.CDS.1	CDS
CmaCh01G001960.1.CDS.2	CmaCh01G001960.1.CDS.2	CDS
CmaCh01G001960.1.CDS.3	CmaCh01G001960.1.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh01G001960.1.three_prime_UTR.1	CmaCh01G001960.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 71..86 score: 4.8E-11coord: 87..107 score: 4.8E-11coord: 53..71 score: 4.8
IPR001623	DnaJ domain	GENE3D	G3DSA:1.10.287.110		coord: 44..128 score: 6.0
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 51..104 score: 2.7
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 50..107 score: 3.2
IPR001623	DnaJ domain	PROFILE	PS50076	DNAJ_2	coord: 51..120 score: 15
IPR001623	DnaJ domain	unknown	SSF46565	Chaperone J-domain	coord: 47..143 score: 1.44
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 2..101 score: 3.2
None	No IPR available	PANTHER	PTHR24078:SF231	CHAPERONE PROTEIN DNAJ	coord: 2..101 score: 3.2