Cla97C07G129040.1 (mRNA) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C07G129040.1
Type	mRNA
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	Inhibitor protein
Location	Cla97Chr07 : 754915 .. 755232 (+)
Sequence length	240

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAAATTGTTATTCCTTCAGGTTAGTAAGACTTCTCCTTCCCACTTGGGTGTTTTCAAATAATTATTTTTTGTTGTCCTTTTCAGTATTTACATTTTCACCATTCAATTTATTTAGGGAAAAATCCTCGTGGCCCGAACTCGTATTCGTCGAATCTTCCACTGCAGTACATATTATAGAGAGAGAATCCTAAAGTGAAGACAATCGTGCTATTAGCTGGATCTCCAGTTACTGAAGATTTGAGATTTGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTATTATTCCTACAATTGGTTGA

mRNA sequence

ATGGCTGAAGAAATTGTTATTCCTTCAGTATTTACATTTTCACCATTCAATTTATTTAGGGAAAAATCCTCGTGGCCCGAACTCGTATTCGTCGAATCTTCCACTGCAAGAGAGAATCCTAAAGTGAAGACAATCGTGCTATTAGCTGGATCTCCAGTTACTGAAGATTTGAGATTTGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTATTATTCCTACAATTGGTTGA

Coding sequence (CDS)

ATGGCTGAAGAAATTGTTATTCCTTCAGTATTTACATTTTCACCATTCAATTTATTTAGGGAAAAATCCTCGTGGCCCGAACTCGTATTCGTCGAATCTTCCACTGCAAGAGAGAATCCTAAAGTGAAGACAATCGTGCTATTAGCTGGATCTCCAGTTACTGAAGATTTGAGATTTGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTATTATTCCTACAATTGGTTGA

Protein sequence

MAEEIVIPSVFTFSPFNLFREKSSWPELVFVESSTARENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIPTIG

Homology

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BLAST of Cla97C07G129040.1 vs. NCBI nr
Match: XP_011654473.1 (PREDICTED: inhibitor of trypsin and hageman factor-like [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_5G027950 [Cucumis sativus])

HSP 1 Score: 77.0 bits (188), Expect = 3.1e-11
Identity = 49/85 (57.65%), Postives = 56/85 (65.88%), Query Frame = 0


Query: 1  MAEE-IVIPSVFTFSPFNLFREKSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVT 60
          MAEE IV PS            KSSWPELVFV+SS A     R+ P VK +VLLAGSPVT
Sbjct: 1  MAEEKIVFPS----------SGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVT 60

Query: 61 EDLRFDRVRVFVNINSVVVIIPTIG 80
          EDLR +RVR+FVN+N  VV +P  G
Sbjct: 61 EDLRPNRVRIFVNMNDRVVEVPRTG 75

[Close]

BLAST of Cla97C07G129040.1 vs. NCBI nr
Match: AES61050.1 (inhibitor of trypsin and hageman factor-like protein [Medicago truncatula])

HSP 1 Score: 68.2 bits (165), Expect = 1.5e-08
Identity = 37/63 (58.73%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPELV VE   A     REN  VK I++L GS VT+DLRFDRVRV+V+   +V  +P
Sbjct: 9  KSSWPELVGVEGKVAEATIQRENASVKAIIVLEGSSVTDDLRFDRVRVWVDKEGIVTQVP 68

[Close]

BLAST of Cla97C07G129040.1 vs. NCBI nr
Match: XP_020423680.1 (glu S.griseus protease inhibitor [Prunus persica] >ONI26577.1 hypothetical protein PRUPE_1G032100 [Prunus persica])

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-07
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 34 KDSWPELVGAEGKVAKATIERENPLVTALILHLGAPITQDLRFDRVRVWVNTNGIVTWIP 93

[Close]

BLAST of Cla97C07G129040.1 vs. NCBI nr
Match: PNX97894.1 (inhibitor of trypsin and hageman factor [Trifolium pratense])

HSP 1 Score: 62.4 bits (150), Expect = 8.0e-07
Identity = 33/68 (48.53%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 17 NLFREKSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSV 76
          N  + K SWPEL+ VE   A     +ENP V  IVLL G+ VT+D R DRVRV+V+   +
Sbjct: 5  NESKGKDSWPELIGVEGKVAAATIEKENPSVNAIVLLEGTAVTDDFRLDRVRVWVDTYGI 64

Query: 77 VVIIPTIG 80
          V+ +P IG
Sbjct: 65 VIEVPKIG 72

[Close]

BLAST of Cla97C07G129040.1 vs. NCBI nr
Match: KMT10314.1 (hypothetical protein BVRB_5g120650 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 62.0 bits (149), Expect = 1.0e-06
Identity = 34/65 (52.31%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 20 REKSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVI 79
          +EK+ WPELV      A     +ENP + TIVLL GSPVT+D R DRVR+FV+   +V  
Sbjct: 5  QEKTQWPELVGENGKEAAKIIEKENPSLHTIVLLIGSPVTKDYRTDRVRIFVDCKGIVGP 64

[Close]

BLAST of Cla97C07G129040.1 vs. TrEMBL
Match: tr|A0A0A0KPI0|A0A0A0KPI0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.1e-11
Identity = 49/85 (57.65%), Postives = 56/85 (65.88%), Query Frame = 0


Query: 1  MAEE-IVIPSVFTFSPFNLFREKSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVT 60
          MAEE IV PS            KSSWPELVFV+SS A     R+ P VK +VLLAGSPVT
Sbjct: 1  MAEEKIVFPS----------SGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVT 60

Query: 61 EDLRFDRVRVFVNINSVVVIIPTIG 80
          EDLR +RVR+FVN+N  VV +P  G
Sbjct: 61 EDLRPNRVRIFVNMNDRVVEVPRTG 75

[Close]

BLAST of Cla97C07G129040.1 vs. TrEMBL
Match: tr|G7I4R4|G7I4R4_MEDTR (Inhibitor of trypsin and hageman factor-like protein OS=Medicago truncatula OX=3880 GN=11430529 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.7e-09
Identity = 37/63 (58.73%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPELV VE   A     REN  VK I++L GS VT+DLRFDRVRV+V+   +V  +P
Sbjct: 9  KSSWPELVGVEGKVAEATIQRENASVKAIIVLEGSSVTDDLRFDRVRVWVDKEGIVTQVP 68

[Close]

BLAST of Cla97C07G129040.1 vs. TrEMBL
Match: tr|A0A251QS16|A0A251QS16_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G032100 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.2e-08
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 34 KDSWPELVGAEGKVAKATIERENPLVTALILHLGAPITQDLRFDRVRVWVNTNGIVTWIP 93

[Close]

BLAST of Cla97C07G129040.1 vs. TrEMBL
Match: tr|M5XN51|M5XN51_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa015379mg PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.2e-08
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 6  KDSWPELVGAEGKVAKATIERENPLVTALILHLGAPITQDLRFDRVRVWVNTNGIVTWIP 65

[Close]

BLAST of Cla97C07G129040.1 vs. TrEMBL
Match: tr|A0A1J3DSG5|A0A1J3DSG5_NOCCA (Protease inhibitor HPI (Fragment) OS=Noccaea caerulescens OX=107243 GN=GA_TR19920_c3_g1_i1_g.66185 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 5.3e-07
Identity = 33/63 (52.38%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 22 KSSWPELV-----FVESSTARENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K++WPELV     +  S   REN +V  +V+L GSPVT D R DRVRVFVN N +VV +P
Sbjct: 18 KNAWPELVGKNGDYAASVIERENTRVDAVVILDGSPVTADFRCDRVRVFVNSNRIVVKVP 77

[Close]

BLAST of Cla97C07G129040.1 vs. Swiss-Prot
Match: sp|P19873|ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.9e-08
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWP LV V  S A     R+NP VK ++L  G+PVT+D R +RVR++VN   +VV  P
Sbjct: 6  KSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPP 65

[Close]

BLAST of Cla97C07G129040.1 vs. Swiss-Prot
Match: sp|Q6XNP7|HPI_HEVBR (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 6.5e-08
Identity = 32/63 (50.79%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K+SWPELV      A      EN  VK IV+  G P+T+DL F+RVRVFV+ N VV  +P
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVP 67

[Close]

BLAST of Cla97C07G129040.1 vs. Swiss-Prot
Match: sp|P24076|BGIA_MOMCH (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.3e-07
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K SWP+LV    + A     RENP+V+ +++  GSPVT D R DRVRV+V    +V   P
Sbjct: 6  KRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARPP 65

[Close]

BLAST of Cla97C07G129040.1 vs. Swiss-Prot
Match: sp|P20076|IER1_SOLLC (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.1e-06
Identity = 30/64 (46.88%), Postives = 40/64 (62.50%), Query Frame = 0


Query: 22  KSSWPELV-----FVESSTARENPKVKTI-VLLAGSPVTEDLRFDRVRVFVNINSVVVII 80
           K SWPEL+     F +    +ENPK+  +  LL GS  TEDLR +RVR+FVN+  +VV  
Sbjct: 56  KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115

[Close]

BLAST of Cla97C07G129040.1 vs. Swiss-Prot
Match: sp|P16231|ICI1_SOLPE (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.7e-06
Identity = 35/79 (44.30%), Postives = 46/79 (58.23%), Query Frame = 0


Query: 6   VIPSVFTFSPFNLFREKSSWPELVFVESSTA-----RENPKVKTI-VLLAGSPVTEDLRF 65
           VI  +  F   +  + K  WPEL+ V +  A     +ENP +  I +LL GSPVT+D R 
Sbjct: 32  VIKLLKEFESDSRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRC 91

Query: 66  DRVRVFVNINSVVVIIPTI 79
           DRVR+FVNI   VV IP +
Sbjct: 92  DRVRLFVNILGDVVQIPRV 110

[Close]

BLAST of Cla97C07G129040.1 vs. TAIR10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 56.6 bits (135), Expect = 8.0e-09
Identity = 33/63 (52.38%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K+SWPEL       A     RENP V   V+L GSPVT D R DRVRVFV+ N +VV  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

[Close]

BLAST of Cla97C07G129040.1 vs. TAIR10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 48.5 bits (114), Expect = 2.2e-06
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPEL+  +   A     RENPK+K +++L G+ V E     RV V+VN   +VV IP
Sbjct: 37 KSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQIP 96

[Close]

BLAST of Cla97C07G129040.1 vs. TAIR10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 41.6 bits (96), Expect = 2.7e-04
Identity = 30/74 (40.54%), Postives = 42/74 (56.76%), Query Frame = 0


Query: 11 FTFSPFNLFREKSSWPELV-----FVESSTARENPKVKTIVLLAGSPVTEDLRFDRVRVF 70
          F FS F++   K+SWPEL+     +  S    EN  +  +V+  G+ VTEDL   RVRV+
Sbjct: 17 FYFSFFDI--GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVW 76

Query: 71 VNINSVVVIIPTIG 80
          V+   +VV  PT G
Sbjct: 77 VDEIRIVVRNPTAG 88

The following BLAST results are available for this feature:

BLAST of Cla97C07G129040.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654473.1	3.1e-11	57.65	PREDICTED: inhibitor of trypsin and hageman factor-like [Cucumis sativus] >KGN49...	[more]
AES61050.1	1.5e-08	58.73	inhibitor of trypsin and hageman factor-like protein [Medicago truncatula]	[more]
XP_020423680.1	1.2e-07	54.84	glu S.griseus protease inhibitor [Prunus persica] >ONI26577.1 hypothetical prote...	[more]
PNX97894.1	8.0e-07	48.53	inhibitor of trypsin and hageman factor [Trifolium pratense]	[more]
KMT10314.1	1.0e-06	52.31	hypothetical protein BVRB_5g120650 [Beta vulgaris subsp. vulgaris]	[more]

BLAST of Cla97C07G129040.1 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KPI0\|A0A0A0KPI0_CUCSA	2.1e-11	57.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=4 SV=1	[more]
tr\|G7I4R4\|G7I4R4_MEDTR	9.7e-09	58.73	Inhibitor of trypsin and hageman factor-like protein OS=Medicago truncatula OX=3...	[more]
tr\|A0A251QS16\|A0A251QS16_PRUPE	8.2e-08	54.84	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G032100 PE=4 SV=1	[more]
tr\|M5XN51\|M5XN51_PRUPE	8.2e-08	54.84	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa015379mg PE=4 SV=1	[more]
tr\|A0A1J3DSG5\|A0A1J3DSG5_NOCCA	5.3e-07	52.38	Protease inhibitor HPI (Fragment) OS=Noccaea caerulescens OX=107243 GN=GA_TR1992...	[more]

BLAST of Cla97C07G129040.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P19873\|ITH5_CUCMA	4.9e-08	50.79	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
sp\|Q6XNP7\|HPI_HEVBR	6.5e-08	50.79	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
sp\|P24076\|BGIA_MOMCH	9.3e-07	46.03	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
sp\|P20076\|IER1_SOLLC	2.1e-06	46.88	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
sp\|P16231\|ICI1_SOLPE	2.7e-06	44.30	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]

BLAST of Cla97C07G129040.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G38870.1	8.0e-09	52.38	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	2.2e-06	46.03	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	2.7e-04	40.54	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036354	Prot_inh_pot1_sf
IPR000864	Prot_inh_pot1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla97C07G129040	Cla97C07G129040	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla97C07G129040.1	Cla97C07G129040.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C07G129040.1.exon.1	Cla97C07G129040.1.exon.1	exon
Cla97C07G129040.1.exon.2	Cla97C07G129040.1.exon.2	exon
Cla97C07G129040.1.exon.3	Cla97C07G129040.1.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla97C07G129040.1.CDS.1	Cla97C07G129040.1.CDS.1	CDS
Cla97C07G129040.1.CDS.2	Cla97C07G129040.1.CDS.2	CDS
Cla97C07G129040.1.CDS.3	Cla97C07G129040.1.CDS.3	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 22..79 e-value: 1.0E-14 score: 54.8
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 21..79
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 24..79 e-value: 2.0E-4 score: 99.0
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 18..79 e-value: 2.8E-18 score: 67.9
None	No IPR available	PANTHER	PTHR33091:SF15	PR-6 PROTEINASE INHIBITOR FAMILY PROTEIN	coord: 21..79
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	SSF54654	CI-2 family of serine protease inhibitors	coord: 20..79