Cla021559.1 (mRNA) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla021559
Type	mRNA
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Protease inhibitor 1 (AHRD V1 ***- D3W0X2_9ROSI); contains Interpro domain(s) IPR000864 Proteinase inhibitor I13, potato inhibitor I
Location	Chr5 : 4136784 .. 4137258 (+)
Sequence length	216

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTACAACATGCAAAGGTAAATGAGTAATATTGCATCATCAAGTCATCTTCGGTTTTTGAACATTGTATTTATTTTAATTTTATCATTTTTTCACAATAATTTTAAATTTTAATTTAAAAAACACAAACTATGCCTATTTAGATTTTGGATTTTTAACACTATATTTATAAACATTATTTTTATATATAGATTTTTTTTTTCATGTTATTCAACCTACACGAGCTTAAATTTATGAAAAAATGAATTAATATCGTTTTGCATGTTTCACTTTTAGGAAAGAGTTCATGGCCGGAATTGGTTGGTAAGGCAGGAAAAGAAGCAGAGAAAATTATTGAGAAGGAGAATCCATTGGTTGACGCCATTATTGTTGATGAAGGATCAATGGTGATTCTAGACTTCAGGTGCGATAGGGTCTGGGTTTGGGTTGATTCCAAAACAGGCATAGTCACTAGGACTCCTTTCATTGGTTAA

mRNA sequence

ATGTCTACAACATGCAAAGGAAAGAGTTCATGGCCGGAATTGGTTGGTAAGGCAGGAAAAGAAGCAGAGAAAATTATTGAGAAGGAGAATCCATTGGTTGACGCCATTATTGTTGATGAAGGATCAATGGTGATTCTAGACTTCAGGTGCGATAGGGTCTGGGTTTGGGTTGATTCCAAAACAGGCATAGTCACTAGGACTCCTTTCATTGGTTAA

Coding sequence (CDS)

ATGTCTACAACATGCAAAGGAAAGAGTTCATGGCCGGAATTGGTTGGTAAGGCAGGAAAAGAAGCAGAGAAAATTATTGAGAAGGAGAATCCATTGGTTGACGCCATTATTGTTGATGAAGGATCAATGGTGATTCTAGACTTCAGGTGCGATAGGGTCTGGGTTTGGGTTGATTCCAAAACAGGCATAGTCACTAGGACTCCTTTCATTGGTTAA

Protein sequence

MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTGIVTRTPFIG

Homology

[Close]

BLAST of Cla021559 vs. Swiss-Prot
Match: BGIA_MOMCH (Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 6.3e-15
Identity = 40/67 (59.70%), Postives = 46/67 (68.66%), Query Frame = 1


Query: 5  CKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTGIV 64
          C+GK SWP+LVG  G  A+ +IE+ENP V A+IV  GS V  DFRCDRV VWV ++ GIV
Sbjct: 3  CQGKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWV-TERGIV 62

Query: 65 TRTPFIG 72
           R P IG
Sbjct: 63 ARPPAIG 68

[Close]

BLAST of Cla021559 vs. Swiss-Prot
Match: ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.3e-14
Identity = 37/69 (53.62%), Postives = 49/69 (71.01%), Query Frame = 1


Query: 3  TTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTG 62
          ++C GKSSWP LVG  G  A+ IIE++NP V A+I++EG+ V  DFRC+RV +WV +K G
Sbjct: 1  SSCPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWV-NKRG 60

Query: 63 IVTRTPFIG 72
          +V   P IG
Sbjct: 61 LVVSPPRIG 68

[Close]

BLAST of Cla021559 vs. Swiss-Prot
Match: ATSI_AMACA (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 7.0e-14
Identity = 39/64 (60.94%), Postives = 43/64 (67.19%), Query Frame = 1


Query: 5  CKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTGIV 64
          C GK  WPELVG+ G +A  IIE+ENP V +I+  E S    DFRCDRVWV VDS TG+V
Sbjct: 4  CPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDS-TGVV 63

Query: 65 TRTP 69
           RTP
Sbjct: 64 VRTP 66

[Close]

BLAST of Cla021559 vs. Swiss-Prot
Match: ICI_LINUS (Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.0e-13
Identity = 37/69 (53.62%), Postives = 45/69 (65.22%), Query Frame = 1


Query: 2  STTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKT 61
          S  C GK++WPELVGK+G  A   +E+EN  V AI++ EGS +  DFRCDRVWV V+   
Sbjct: 1  SRRCPGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDH- 60

Query: 62 GIVTRTPFI 71
          G+VT  P I
Sbjct: 61 GVVTSVPHI 68

[Close]

BLAST of Cla021559 vs. Swiss-Prot
Match: ITI_FAGTA (Trypsin inhibitor OS=Fagopyrum tataricum PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.2e-09
Identity = 33/72 (45.83%), Postives = 41/72 (56.94%), Query Frame = 1


Query: 4  TCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVI----LDFRCDRVWVWVDS 63
          T  GK SWPELVG  GK A   I+KEN  V A++    + +      DFRCDRV V ++ 
Sbjct: 15 TYVGKQSWPELVGTKGKTAAATIDKENTHVTAVLCPPLTTLAACRTFDFRCDRVRVLINR 74

Query: 64 KTGIVTRTPFIG 72
            G+VT+TP +G
Sbjct: 75 IGGVVTKTPTVG 86

[Close]

BLAST of Cla021559 vs. TrEMBL
Match: A0A0A0L795_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142960 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 3.7e-30
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MSTTCKGKSSWPELVGKAGKEAEKIIEKENP VDA+IVDEGS+V  DFRCDRVWVWVDSK
Sbjct: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSK 60

Query: 61 TGIVTRTPFIG 72
          T IVTRTPFIG
Sbjct: 61 TSIVTRTPFIG 71

[Close]

BLAST of Cla021559 vs. TrEMBL
Match: U5G1C3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s08600g PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.0e-20
Identity = 52/69 (75.36%), Postives = 57/69 (82.61%), Query Frame = 1


Query: 3  TTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTG 62
          TTC+GKSSWPEL+G  GK A   IE+ENPLV+AIIV EGSMVILDFRCDRVWVWVD K G
Sbjct: 5  TTCQGKSSWPELLGAEGKVAAATIERENPLVEAIIVPEGSMVILDFRCDRVWVWVD-KRG 64

Query: 63 IVTRTPFIG 72
          IV + P IG
Sbjct: 65 IVFQVPGIG 72

[Close]

BLAST of Cla021559 vs. TrEMBL
Match: B1ACD2_SOYBN (Putative protease inhibitor OS=Glycine max GN=GLYMA_10G184700 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.0e-20
Identity = 52/71 (73.24%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MS  CKGKSSWPELVG  G  AE  IE+ENPLVDAIIV EG+MVI DFRCDRVWVW+D K
Sbjct: 1  MSDDCKGKSSWPELVGVQGTVAEATIERENPLVDAIIVPEGNMVITDFRCDRVWVWID-K 60

Query: 61 TGIVTRTPFIG 72
           GIV   P IG
Sbjct: 61 DGIVKEVPHIG 70

[Close]

BLAST of Cla021559 vs. TrEMBL
Match: A0A0B2P035_GLYSO (Inhibitor of trypsin and hageman factor OS=Glycine soja GN=glysoja_000750 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.0e-20
Identity = 52/71 (73.24%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MS  CKGKSSWPELVG  G  AE  IE+ENPLVDAIIV EG+MVI DFRCDRVWVW+D K
Sbjct: 1  MSDDCKGKSSWPELVGVQGTVAEATIERENPLVDAIIVPEGNMVITDFRCDRVWVWID-K 60

Query: 61 TGIVTRTPFIG 72
           GIV   P IG
Sbjct: 61 DGIVKEVPHIG 70

[Close]

BLAST of Cla021559 vs. TrEMBL
Match: A0A0A0L4J0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142970 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.5e-20
Identity = 50/71 (70.42%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MS  CKGKSSWPELVG +G  AEKIIEKEN  V+AIIV++G  V  DFRCDRVWVWVD  
Sbjct: 1  MSIDCKGKSSWPELVGVSGNIAEKIIEKENCYVNAIIVEQGKFVTQDFRCDRVWVWVDKH 60

Query: 61 TGIVTRTPFIG 72
          T IV +TP IG
Sbjct: 61 THIVIKTPIIG 71

[Close]

BLAST of Cla021559 vs. NCBI nr
Match: gi|449432606|ref|XP_004134090.1| (PREDICTED: glu S.griseus protease inhibitor-like [Cucumis sativus])

HSP 1 Score: 138.3 bits (347), Expect = 5.3e-30
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MSTTCKGKSSWPELVGKAGKEAEKIIEKENP VDA+IVDEGS+V  DFRCDRVWVWVDSK
Sbjct: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPSVDAVIVDEGSVVTQDFRCDRVWVWVDSK 60

Query: 61 TGIVTRTPFIG 72
          T IVTRTPFIG
Sbjct: 61 TSIVTRTPFIG 71

[Close]

BLAST of Cla021559 vs. NCBI nr
Match: gi|659076233|ref|XP_008438570.1| (PREDICTED: glu S.griseus protease inhibitor-like [Cucumis melo])

HSP 1 Score: 137.9 bits (346), Expect = 6.9e-30
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MSTTCKGKSSWPELVGKAGKEAEK IE+ENPLVDAIIVDEGS+VI DFRCDRVWVWVD K
Sbjct: 1  MSTTCKGKSSWPELVGKAGKEAEKTIERENPLVDAIIVDEGSVVIQDFRCDRVWVWVDPK 60

Query: 61 TGIVTRTPFIG 72
          T IVTRTPFIG
Sbjct: 61 TAIVTRTPFIG 71

[Close]

BLAST of Cla021559 vs. NCBI nr
Match: gi|351727373|ref|NP_001238694.1| (protease inhibitor-like [Glycine max])

HSP 1 Score: 105.9 bits (263), Expect = 2.9e-20
Identity = 52/71 (73.24%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 1  MSTTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSK 60
          MS  CKGKSSWPELVG  G  AE  IE+ENPLVDAIIV EG+MVI DFRCDRVWVW+D K
Sbjct: 1  MSDDCKGKSSWPELVGVQGTVAEATIERENPLVDAIIVPEGNMVITDFRCDRVWVWID-K 60

Query: 61 TGIVTRTPFIG 72
           GIV   P IG
Sbjct: 61 DGIVKEVPHIG 70

[Close]

BLAST of Cla021559 vs. NCBI nr
Match: gi|566189800|ref|XP_006378352.1| (hypothetical protein POPTR_0010s08600g [Populus trichocarpa])

HSP 1 Score: 105.9 bits (263), Expect = 2.9e-20
Identity = 52/69 (75.36%), Postives = 57/69 (82.61%), Query Frame = 1


Query: 3  TTCKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTG 62
          TTC+GKSSWPEL+G  GK A   IE+ENPLV+AIIV EGSMVILDFRCDRVWVWVD K G
Sbjct: 5  TTCQGKSSWPELLGAEGKVAAATIERENPLVEAIIVPEGSMVILDFRCDRVWVWVD-KRG 64

Query: 63 IVTRTPFIG 72
          IV + P IG
Sbjct: 65 IVFQVPGIG 72

[Close]

BLAST of Cla021559 vs. NCBI nr
Match: gi|357441047|ref|XP_003590801.1| (inhibitor of trypsin and hageman factor-like protein [Medicago truncatula])

HSP 1 Score: 104.8 bits (260), Expect = 6.5e-20
Identity = 52/67 (77.61%), Postives = 55/67 (82.09%), Query Frame = 1


Query: 5  CKGKSSWPELVGKAGKEAEKIIEKENPLVDAIIVDEGSMVILDFRCDRVWVWVDSKTGIV 64
          CKGKSSWPELVG  GK AE  IE+ENPLV+AIIV EGS VILDFRCDRVWVWVD K GIV
Sbjct: 7  CKGKSSWPELVGVEGKVAEATIERENPLVNAIIVPEGSAVILDFRCDRVWVWVD-KDGIV 66

Query: 65 TRTPFIG 72
           + P IG
Sbjct: 67 FKVPTIG 72

The following BLAST results are available for this feature:

BLAST of Cla021559 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BGIA_MOMCH	6.3e-15	59.70	Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1	[more]
ITH5_CUCMA	5.3e-14	53.62	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1	[more]
ATSI_AMACA	7.0e-14	60.94	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus PE=1 SV=1	[more]
ICI_LINUS	2.0e-13	53.62	Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1	[more]
ITI_FAGTA	5.2e-09	45.83	Trypsin inhibitor OS=Fagopyrum tataricum PE=1 SV=1	[more]

BLAST of Cla021559 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L795_CUCSA	3.7e-30	91.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142960 PE=4 SV=1	[more]
U5G1C3_POPTR	2.0e-20	75.36	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s08600g PE=4 SV=1	[more]
B1ACD2_SOYBN	2.0e-20	73.24	Putative protease inhibitor OS=Glycine max GN=GLYMA_10G184700 PE=2 SV=1	[more]
A0A0B2P035_GLYSO	2.0e-20	73.24	Inhibitor of trypsin and hageman factor OS=Glycine soja GN=glysoja_000750 PE=4 S...	[more]
A0A0A0L4J0_CUCSA	4.5e-20	70.42	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142970 PE=4 SV=1	[more]

BLAST of Cla021559 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432606\|ref\|XP_004134090.1\|	5.3e-30	91.55	PREDICTED: glu S.griseus protease inhibitor-like [Cucumis sativus]	[more]
gi\|659076233\|ref\|XP_008438570.1\|	6.9e-30	91.55	PREDICTED: glu S.griseus protease inhibitor-like [Cucumis melo]	[more]
gi\|351727373\|ref\|NP_001238694.1\|	2.9e-20	73.24	protease inhibitor-like [Glycine max]	[more]
gi\|566189800\|ref\|XP_006378352.1\|	2.9e-20	75.36	hypothetical protein POPTR_0010s08600g [Populus trichocarpa]	[more]
gi\|357441047\|ref\|XP_003590801.1\|	6.5e-20	77.61	inhibitor of trypsin and hageman factor-like protein [Medicago truncatula]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000864	Prot_inh_pot1

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cla021559	Cla021559	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cla021559	Cla021559.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cla021559.1.cds1	Cla021559.1.cds1	CDS
Cla021559.1.cds2	Cla021559.1.cds2	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 10..71 score: 4.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 10..23 score: 5.2E-7coord: 47..57 score: 5.2E-7coord: 35..46 score: 5.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 8..71 score: 5.6
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 11..22 scor
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 4..71 score: 1.14
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 4..71 score: 3.4
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 1..71 score: 4.7
None	No IPR available	PANTHER	PTHR33091:SF14	SUBFAMILY NOT NAMED	coord: 1..71 score: 4.7