ClCG02G012910.1 (mRNA) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG02G012910.1
Type	mRNA
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	PR-6 proteinase inhibitor family protein
Location	CG_Chr02 : 26657941 .. 26658445 (+)
Sequence length	282

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGGTATTCGTACTTGTAGCTCTCCTCCTTACCCACCTTGTGTAAGTGATGGTTGCATGGATCCTCAATGTTGCGGTTAGAGCCTTTATCCCTCTTTTAATCAAAATTCGTTCCACCTCTAATTTTCTTATTAGATGAGATAATATTACATTTTAAGCAATTGTTTAAATTAGAATACAAATTATGAATATGGTACGAAAAATATCAAATACAGTTATATATGTGTTTATATTGAATCTCTTGTTTTTTAAATTGTCCATCAAATTGGAGGAATAATTACTAACGTTGAGTTTTGTAGATGCAGGAGGGAATTTTAGATGGCCTAAACTAGTAGGTAAGAAGGGAGATTATGCTAAAAGTGAAATAGAAAAGGATGTGCGTTGTGTGAGGGTTGTAATTTTAAGACAAGGCACGGTTAGAATTGAAAATTTCTGTTGCAATCGTGTTTTTGTTTACGTGGATGATAGTGGCCACGTGGTTGATGTCCCAACCATTGGTTGA

mRNA sequence

ATGGCAGGTATTCGTACTTGTAGCTCTCCTCCTTACCCACCTTGTGTAAGTGATGGTTGCATGGATCCTCAATGTTGCGATGCAGGAGGGAATTTTAGATGGCCTAAACTAGTAGGTAAGAAGGGAGATTATGCTAAAAGTGAAATAGAAAAGGATGTGCGTTGTGTGAGGGTTGTAATTTTAAGACAAGGCACGGTTAGAATTGAAAATTTCTGTTGCAATCGTGTTTTTGTTTACGTGGATGATAGTGGCCACGTGGTTGATGTCCCAACCATTGGTTGA

Coding sequence (CDS)

ATGGCAGGTATTCGTACTTGTAGCTCTCCTCCTTACCCACCTTGTGTAAGTGATGGTTGCATGGATCCTCAATGTTGCGATGCAGGAGGGAATTTTAGATGGCCTAAACTAGTAGGTAAGAAGGGAGATTATGCTAAAAGTGAAATAGAAAAGGATGTGCGTTGTGTGAGGGTTGTAATTTTAAGACAAGGCACGGTTAGAATTGAAAATTTCTGTTGCAATCGTGTTTTTGTTTACGTGGATGATAGTGGCCACGTGGTTGATGTCCCAACCATTGGTTGA

Protein sequence

MAGIRTCSSPPYPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVVDVPTIG

Homology

[Close]

BLAST of ClCG02G012910.1 vs. Swiss-Prot
Match: ICI_LINUS (Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.4e-08
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1


Query: 30 GNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVVDV 89
          G   WP+LVGK G+ A + +E++ R V  ++L++G+   ++F C+RV+V V+D G V  V
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 90 PTI 93
          P I
Sbjct: 66 PHI 68

[Close]

BLAST of ClCG02G012910.1 vs. Swiss-Prot
Match: ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 8.3e-07
Identity = 29/64 (45.31%), Postives = 37/64 (57.81%), Query Frame = 1


Query: 30 GNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVVDV 89
          G   WP LVG  G  AK+ IE+    V+ VIL +GT   ++F CNRV ++V+  G VV  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 90 PTIG 94
          P IG
Sbjct: 65 PRIG 68

[Close]

BLAST of ClCG02G012910.1 vs. Swiss-Prot
Match: BGIA_MOMCH (Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.1e-06
Identity = 28/64 (43.75%), Postives = 36/64 (56.25%), Query Frame = 1


Query: 30 GNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVVDV 89
          G   WP+LVG  G  AK+ IE++   VR VI+R G+    +F C+RV V+V + G V   
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 90 PTIG 94
          P IG
Sbjct: 65 PAIG 68

[Close]

BLAST of ClCG02G012910.1 vs. TrEMBL
Match: V4NI49_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10011055mg PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.2e-20
Identity = 47/82 (57.32%), Postives = 57/82 (69.51%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S GC DP+CC  GG F WP+LVGK G+ AK  IE++   V   I+  GT+RIE+F
Sbjct: 7  YPPCESSGCTDPRCCALGGKFEWPELVGKSGEIAKMTIERENPNVLGFIMPYGTLRIEDF 66

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVF+ V   GHVV +P IG
Sbjct: 67 CCNRVFIVVGSKGHVVMIPKIG 88

[Close]

BLAST of ClCG02G012910.1 vs. TrEMBL
Match: Q9STF8_ARATH (PR-6 proteinase inhibitor family protein OS=Arabidopsis thaliana GN=T6H20.110 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 3.5e-20
Identity = 46/82 (56.10%), Postives = 55/82 (67.07%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S  C DP+CC  G  +RWP+LVGK G  AK  IE++   V  ++LR G  RIENF
Sbjct: 4  YPPCWSGSCEDPECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVFVY+  +G V D P IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of ClCG02G012910.1 vs. TrEMBL
Match: A0MF11_ARATH (Putative uncharacterized protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.6e-19
Identity = 45/82 (54.88%), Postives = 54/82 (65.85%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S  C DP+C   G  +RWP+LVGK G  AK  IE++   V  ++LR G  RIENF
Sbjct: 4  YPPCWSGSCEDPECRAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVFVY+  +G V D P IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of ClCG02G012910.1 vs. TrEMBL
Match: R0G9B1_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10015502mg PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.6e-17
Identity = 42/83 (50.60%), Postives = 54/83 (65.06%), Query Frame = 1


Query: 11 PYPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIEN 70
          PYPPC+   C DP+CC  G   RWP+LVGK G+ AK  IE++   V  ++L  G  RI +
Sbjct: 3  PYPPCLVTACEDPECCANGKKCRWPELVGKSGEVAKMTIERENPNVLAILLHAGDGRISD 62

Query: 71 FCCNRVFVYVDDSGHVVDVPTIG 94
          FCCN+VFV +D  GHV  +P IG
Sbjct: 63 FCCNKVFVVLDIHGHVSTIPKIG 85

[Close]

BLAST of ClCG02G012910.1 vs. TrEMBL
Match: A0A0A0LJT7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G287050 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.6e-17
Identity = 42/66 (63.64%), Postives = 54/66 (81.82%), Query Frame = 1


Query: 28 AGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVV 87
          AGGN+RWP LVGK+  YAK +IE+++  V V ++R+G +RIE+FCCNRV VYVDDSG VV
Sbjct: 7  AGGNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVV 66

Query: 88 DVPTIG 94
          +VP IG
Sbjct: 67 EVPVIG 72

[Close]

BLAST of ClCG02G012910.1 vs. TAIR10
Match: AT3G46860.1 (AT3G46860.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 105.5 bits (262), Expect = 1.8e-23
Identity = 46/82 (56.10%), Postives = 53/82 (64.63%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S  C DP+CC  G  +RWP+LVGK G  AK  IE++   V  ++LR G  RIENF
Sbjct: 4  YPPCWSGSCEDPECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVFVY+  +G V D P IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of ClCG02G012910.1 vs. TAIR10
Match: AT5G43580.1 (AT5G43580.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 64.7 bits (156), Expect = 3.4e-11
Identity = 31/68 (45.59%), Postives = 43/68 (63.24%), Query Frame = 1


Query: 26 CDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGH 85
          C+  G   WP+L+G KG+ AK  IE++   ++ VI+  GTV  E F C+RV+V+V+D G 
Sbjct: 32 CEDPGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGI 91

Query: 86 VVDVPTIG 94
          VV +P IG
Sbjct: 92 VVQIPIIG 99

[Close]

BLAST of ClCG02G012910.1 vs. TAIR10
Match: AT2G38900.2 (AT2G38900.2 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 49.7 bits (117), Expect = 1.1e-06
Identity = 26/64 (40.62%), Postives = 34/64 (53.12%), Query Frame = 1


Query: 30 GNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVVDV 89
          G   WP+L+G  GDYA S I+ +   + VV++  G    E+  C RV V+VD+   VV  
Sbjct: 25 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 90 PTIG 94
          PT G
Sbjct: 85 PTAG 88

[Close]

BLAST of ClCG02G012910.1 vs. TAIR10
Match: AT2G38870.1 (AT2G38870.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 46.6 bits (109), Expect = 9.7e-06
Identity = 23/60 (38.33%), Postives = 31/60 (51.67%), Query Frame = 1


Query: 34 WPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVVDVPTIG 93
          WP+L G  GDYA   IE++   V   ++  G+    +F C+RV V+VD +  VV  P  G
Sbjct: 11 WPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKSG 70

[Close]

BLAST of ClCG02G012910.1 vs. NCBI nr
Match: gi|567189624|ref|XP_006404448.1| (hypothetical protein EUTSA_v10011055mg [Eutrema salsugineum])

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-20
Identity = 47/82 (57.32%), Postives = 57/82 (69.51%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S GC DP+CC  GG F WP+LVGK G+ AK  IE++   V   I+  GT+RIE+F
Sbjct: 7  YPPCESSGCTDPRCCALGGKFEWPELVGKSGEIAKMTIERENPNVLGFIMPYGTLRIEDF 66

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVF+ V   GHVV +P IG
Sbjct: 67 CCNRVFIVVGSKGHVVMIPKIG 88

[Close]

BLAST of ClCG02G012910.1 vs. NCBI nr
Match: gi|15232657|ref|NP_190270.1| (PR-6 proteinase inhibitor family protein [Arabidopsis thaliana])

HSP 1 Score: 105.5 bits (262), Expect = 5.0e-20
Identity = 46/82 (56.10%), Postives = 55/82 (67.07%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S  C DP+CC  G  +RWP+LVGK G  AK  IE++   V  ++LR G  RIENF
Sbjct: 4  YPPCWSGSCEDPECCAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVFVY+  +G V D P IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of ClCG02G012910.1 vs. NCBI nr
Match: gi|116831272|gb|ABK28590.1| (unknown [Arabidopsis thaliana])

HSP 1 Score: 100.9 bits (250), Expect = 1.2e-18
Identity = 45/82 (54.88%), Postives = 54/82 (65.85%), Query Frame = 1


Query: 12 YPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENF 71
          YPPC S  C DP+C   G  +RWP+LVGK G  AK  IE++   V  ++LR G  RIENF
Sbjct: 4  YPPCWSGSCEDPECRAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENF 63

Query: 72 CCNRVFVYVDDSGHVVDVPTIG 94
          CCNRVFVY+  +G V D P IG
Sbjct: 64 CCNRVFVYLGSNGQVADAPMIG 85

[Close]

BLAST of ClCG02G012910.1 vs. NCBI nr
Match: gi|565483408|ref|XP_006299344.1| (hypothetical protein CARUB_v10015502mg [Capsella rubella])

HSP 1 Score: 96.7 bits (239), Expect = 2.3e-17
Identity = 42/83 (50.60%), Postives = 54/83 (65.06%), Query Frame = 1


Query: 11 PYPPCVSDGCMDPQCCDAGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIEN 70
          PYPPC+   C DP+CC  G   RWP+LVGK G+ AK  IE++   V  ++L  G  RI +
Sbjct: 3  PYPPCLVTACEDPECCANGKKCRWPELVGKSGEVAKMTIERENPNVLAILLHAGDGRISD 62

Query: 71 FCCNRVFVYVDDSGHVVDVPTIG 94
          FCCN+VFV +D  GHV  +P IG
Sbjct: 63 FCCNKVFVVLDIHGHVSTIPKIG 85

[Close]

BLAST of ClCG02G012910.1 vs. NCBI nr
Match: gi|700206904|gb|KGN62023.1| (hypothetical protein Csa_2G287050 [Cucumis sativus])

HSP 1 Score: 95.5 bits (236), Expect = 5.2e-17
Identity = 42/66 (63.64%), Postives = 54/66 (81.82%), Query Frame = 1


Query: 28 AGGNFRWPKLVGKKGDYAKSEIEKDVRCVRVVILRQGTVRIENFCCNRVFVYVDDSGHVV 87
          AGGN+RWP LVGK+  YAK +IE+++  V V ++R+G +RIE+FCCNRV VYVDDSG VV
Sbjct: 7  AGGNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVV 66

Query: 88 DVPTIG 94
          +VP IG
Sbjct: 67 EVPVIG 72

The following BLAST results are available for this feature:

BLAST of ClCG02G012910.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ICI_LINUS	3.4e-08	41.27	Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1	[more]
ITH5_CUCMA	8.3e-07	45.31	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1	[more]
BGIA_MOMCH	9.1e-06	43.75	Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1	[more]

BLAST of ClCG02G012910.1 vs. TrEMBL


Match Name	E-value	Identity	Description
V4NI49_EUTSA	1.2e-20	57.32	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10011055mg PE=4 SV=1	[more]
Q9STF8_ARATH	3.5e-20	56.10	PR-6 proteinase inhibitor family protein OS=Arabidopsis thaliana GN=T6H20.110 PE...	[more]
A0MF11_ARATH	8.6e-19	54.88	Putative uncharacterized protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1	[more]
R0G9B1_9BRAS	1.6e-17	50.60	Uncharacterized protein OS=Capsella rubella GN=CARUB_v10015502mg PE=4 SV=1	[more]
A0A0A0LJT7_CUCSA	3.6e-17	63.64	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G287050 PE=4 SV=1	[more]

BLAST of ClCG02G012910.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G46860.1	1.8e-23	56.10	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	3.4e-11	45.59	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	1.1e-06	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	9.7e-06	38.33	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

BLAST of ClCG02G012910.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|567189624\|ref\|XP_006404448.1\|	1.7e-20	57.32	hypothetical protein EUTSA_v10011055mg [Eutrema salsugineum]	[more]
gi\|15232657\|ref\|NP_190270.1\|	5.0e-20	56.10	PR-6 proteinase inhibitor family protein [Arabidopsis thaliana]	[more]
gi\|116831272\|gb\|ABK28590.1\|	1.2e-18	54.88	unknown [Arabidopsis thaliana]	[more]
gi\|565483408\|ref\|XP_006299344.1\|	2.3e-17	50.60	hypothetical protein CARUB_v10015502mg [Capsella rubella]	[more]
gi\|700206904\|gb\|KGN62023.1\|	5.2e-17	63.64	hypothetical protein Csa_2G287050 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000864	Prot_inh_pot1

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009611	response to wounding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
ClCG02G012910	ClCG02G012910	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
ClCG02G012910.1	ClCG02G012910.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
ClCG02G012910.1.cds1	ClCG02G012910.1.cds1	CDS
ClCG02G012910.1.cds2	ClCG02G012910.1.cds2	CDS

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRODOM	PD002604		coord: 34..92 score: 3.
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 33..93 score: 2.0
IPR000864	Proteinase inhibitor I13, potato inhibitor I	unknown	SSF54654	CI-2 family of serine protease inhibitors	coord: 28..93 score: 3.79
None	No IPR available	GENE3D	G3DSA:3.30.10.10		coord: 32..93 score: 2.1
None	No IPR available	PANTHER	PTHR33091	FAMILY NOT NAMED	coord: 1..93 score: 4.5
None	No IPR available	PANTHER	PTHR33091:SF13	PR-6 PROTEINASE INHIBITOR FAMILY PROTEIN-RELATED	coord: 1..93 score: 4.5