Carg28070-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg28070-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cysteine proteinase inhibitor
Location	Cucurbita_argyrosperma_scaffold_169 : 298571 .. 302319 (-)
Sequence length	660

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCAACCAACGATCCACACTAAAAAATTCACTCAATTTCTTCCTCCAATTATCAACTTCGCTCTTACAAAGTCCTGTGTTCCAATCCCAGAAAGCGACCATGAAAGTGAATCACATAGCTGCGGTGTTGCTGCTGCTTCTTCTTCCTCTGTTTGATTCGGGGTATTGCGTTGAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAACACAACAAGAAAGAGGTTTTTTCCTTTGTTTTCTTTGGTTTTTCAGCTCTTTATTCATTAATTTCTGGATTCGTTTCACTGCCTAATTTTCTTTCTTATTTTTGCTCAGAATCTAGTCACTTGCTTATTTAATTCGTTAATTAGTGGCTATTTGGTACCCATGTTTTGGTTGTTCATGCGTCGTTTTGATTTGTTAATCACCACTAGATGCGGTAGTTTAAATCGAATTCTTGACAGGCTTTTATGTCTCTTTGGTGCAGAATGGTCTTCTGGAATTTGAACGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCATTGATGGTGGTATAAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGATGGATCTTCAGGTGCCGTTCTGCTCCTCGTGTTCAAACTGGTATGGTAGGAATCGTTCTGTTCTATGTTAATTGTATATTTTGTATATCTGCATGACAATACTAGACTATTAGTCCATTTATGGTAGGAACCTAAGATAGCCTGTCTATGTCTGACACATTAGACACCTTTTTGGACTTTTTGTAACGGCCCAAGCCTACTGTTAGCAGATATTGTCCTCTTTAGGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAGAACGCGTTTGCTAGGGAAAGGTCTCCACACCCTTATAAAAGGTGTTTTGTTCTTCTCCCCAACCGATATGGGATCTCACACTTGTGATGGACACTTGTTAGTACAATAAATATATGTTATACAAAATCAATATAGGTCCAACATTTGTTAGACACGTACTGATCACTTGCTAAGTATATCAGTATAATAAAATGATAATAGAAAAATACATCACACATTGATTTAGTTATAAATGCATAAACTTTTTCGTTCTGAATTTTCTTATGCCATTGAAATGATATATATTTTAATAAATGTGTTCTTGTTGTGTACGTGTCCTAAATTTTTTAAAACATGTTGTGTCACTATGGCTGTGTCGTGTTTTGTACTTTGTATTTGTATTCGTGCTTCTTAGCTTATAATTTGGTATATGTACATGTGTAAATGTCAATAATTTGAGTTTTTGCTTTTGAAAACTTTTCTGAAAAGTTGAAGAAAGATAAAGGGTGTGAGACTAGTTAATCCTCTTTTTCTTATTCTTGTTTGTTATAATTGCTGCTGTGCTTGGTAGGATGATTGCAATGAGAGATCGATTCACTCTTGCTGAGCACCACTGCTCACAACAGAGAAAATGGCTCTCACACACTGTTATCAAAATAACTAACTATGTTAACGATACCAAAATGGTACGGAGGGATTAATGGTGAGTCCAAGTCAGTCCCAAACTAAATTGAATAAGAAATCTTTGTATCCTTAGTGTTGAATGTATTTGAAGCCAAATCAAGTGTTAGTATTGATAGATTTAAGTGATTTGGTTGTAACGGCCCAAGCTCACCGCTAGCAGATATTGTCCACTTTGGGCTTTTCTTTCTGGGATTCCCCTCAAGGTTTTTAAAACGCGTTTGCTAGGGAAAGGTTTTCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAACCGTTGTAGGATCTCACAATCTACCCCTCTTCGGGGTCCAGCGTCCTCACTAGCACTCGTTCTCTTCTCCAATCAATGTGGAACCTCCAATCTACCATTTTCGGGGCCCAGTGTCCTTGTTGGCACACCGCCTCATGTTCGCCCCCCTTTGGGGCTCAGTCTCCTTGCTGGCACTTTGTCCGGTGTTTGGCTCTGATACCATTGTAACGGTCCAAGTCCACCGCTAGCAGATATTTCCTCTTTGGGCTTTCCCTTTCGGGCTTTCCCTCAAGGTTTTTAAAACGCGTCTGCAAGGGAGAGATTTCTACACCCTTATAAAGAATACTTCGTTCTCCTCTCCAACAGATGTGGATCTCACATTAGTGTTTGAGTTTAATTAAGTCAAAGGGAAACAAGAATTTGGTGGGTTGTGTGGTCTCTCCATTTTTGTTATGTTATTTTGGTCTTCCATGGTCATCAGGAAGCATGAGAGATTTTAAAAAAATTTTCTTTGGATCATCTTAATGAATTTGATTAGTAAAAACACTTCCATTTGGCCAGTAGTTGTCTGCTAGTGTTCCCTGGAATCTAAGACTCTTTGGTATAAGATTATTGTTAGTTTGCATATCTTCTGGTTTTGAGGTCAGATCGATTTTTCTTTTGAGAATGGAGCTCGTGTTAACTTCTGGGGAGGATCGATTGATTCTAGTCGTTAACACCGTATATACCATCTAGAAGTTTATTATGTTCTTCTTCGTCTGGTTGTGTATTATCTATAAGGAGCAGATTAAATACTATAGGATTGTGTTTTGTGGATTTTATTTTTTTCAACATGGATTGGATATGGCTATAGCTCATCTTAAAGATTTTCTTGAGCAAGTAGTTGCAAGGATACCTCCTCATGGGAACTGCCACTTTAACAAACGACATGGAATATTTTAGAGGGGCAAAAATAAACATAGTATGACGTAGAATCTTATGCTGCGTCGTCTCAGTTTTATATATATATATATGCAAGCATTCAATTTATCAGTATATCATGTCAACTTGATCCCACATCGAAACACTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGCGTTATAAAATCGTGAAACTGACGGTGGTACATAACGAGCTAAATAGAACAATATCTGCTAGCAGTGGGCTTGGGCCGTTACAAATGGCAGTAGAGCTAGACACTGGGGATGTGCCAACAAAGATGTTGGGCCCCGAAGGGGGGTGAATTTCGTGGGGGTCTCGCATCGATTGGAGAAAGGAACGAGTGCCAACGAGGACGCTGGGCCTCGGAGGGGGGTGGATTGTGATATCCCACATTGGTTTGGGAGGACAACGAAACACCTTTTATAAGGGTGTGGAAACCTCTCCCTAACTTTGAGGGGAAGTGTGGAAGGGAAAGTTCACATAGGACAATATCGGCTAGTAGTGGGCTTGGACCGTTACACTTTCATTCTTAAATTGGTTTTTGTGTTACTGTCTAGCTAGAAATTTATCTTACAATAAATTGATATATTCATCTAGATGTTCAAATAAGGGCGATATGCATCAAAATAAGAACTATAGTTGTAATAATGTAACCAAAATTTATGTGTTATGTTTCGTGCATTGTTAAAATGGAGTCGAGAGTCGTATTGTGTCGTAAGATCGATTTCCGCTATGTTGATTGTTGTTCTTGGATGGCAGGTAAGTGAAGAGTATACATAAACATACCCTATAGCCCATGTGGGTTGCTGCTGAAGGTAAACAACAGAATAGATCTGAAACATTATAGGAACTCTGTTATATGATATGTTTATAATAATGGTGTAATATATATATATATATATATATATG

mRNA sequence

ATTTCAACCAACGATCCACACTAAAAAATTCACTCAATTTCTTCCTCCAATTATCAACTTCGCTCTTACAAAGTCCTGTGTTCCAATCCCAGAAAGCGACCATGAAAGTGAATCACATAGCTGCGGTGTTGCTGCTGCTTCTTCTTCCTCTGTTTGATTCGGGGTATTGCGTTGAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAACACAACAAGAAAGAGAATGGTCTTCTGGAATTTGAACGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCATTGATGGTGGTATAAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGATGGATCTTCAGGTGCCGTTCTGCTCCTCGTGTTCAAACTGGTAAGTGAAGAGTATACATAAACATACCCTATAGCCCATGTGGGTTGCTGCTGAAGGTAAACAACAGAATAGATCTGAAACATTATAGGAACTCTGTTATATGATATGTTTATAATAATGGTGTAATATATATATATATATATATATATG

Coding sequence (CDS)

ATGAAAGTGAATCACATAGCTGCGGTGTTGCTGCTGCTTCTTCTTCCTCTGTTTGATTCGGGGTATTGCGTTGAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAACACAACAAGAAAGAGAATGGTCTTCTGGAATTTGAACGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCATTGATGGTGGTATAAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGA

Protein sequence

MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKPCP

Homology

[Close]

BLAST of Carg28070-RA vs. NCBI nr
Match: XP_022932782.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932784.1 cysteine proteinase inhibitor A-like [Cucurbita moschata])

HSP 1 Score: 248.8 bits (634), Expect = 9.3e-63
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
           KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 123
           CP
Sbjct: 121 CP 122

[Close]

BLAST of Carg28070-RA vs. NCBI nr
Match: XP_022967919.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967921.1 cysteine proteinase inhibitor A-like [Cucurbita maxima])

HSP 1 Score: 241.5 bits (615), Expect = 1.5e-60
Identity = 117/121 (96.69%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHIAAVLL+LLLP+FDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLVLLLPVFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
           KKENGLLEFERVLKAKEQVV+GKLYHLTLEAIDGG+KKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 C 122
           C
Sbjct: 121 C 121

[Close]

BLAST of Carg28070-RA vs. NCBI nr
Match: XP_023543498.1 (cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543501.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.8 bits (608), Expect = 9.6e-60
Identity = 115/122 (94.26%), Postives = 120/122 (98.36%), Query Frame = 0


Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHI A+LLLLLLP+FDSGYCV+DPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQVVAGKLYHLTLEA DGG+KKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 123
           CP
Sbjct: 121 CP 122

[Close]

BLAST of Carg28070-RA vs. NCBI nr
Match: XP_008455746.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901819.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo])

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-50
Identity = 101/121 (83.47%), Postives = 110/121 (90.91%), Query Frame = 0


Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ +EDPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
            KENGLLEF+RVLKAKEQVVAGKLYHLTLEAIDGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 C 122
           C
Sbjct: 121 C 121

[Close]

BLAST of Carg28070-RA vs. NCBI nr
Match: XP_011041371.1 (PREDICTED: cysteine proteinase inhibitor 6-like [Populus euphratica])

HSP 1 Score: 167.5 bits (423), Expect = 2.7e-38
Identity = 85/114 (74.56%), Postives = 94/114 (82.46%), Query Frame = 0


Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCRQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLTLEAID G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTLEAIDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]

BLAST of Carg28070-RA vs. TAIR10
Match: AT3G12490.2 (cystatin B)

HSP 1 Score: 139.4 bits (350), Expect = 1.4e-33
Identity = 68/102 (66.67%), Postives = 82/102 (80.39%), Query Frame = 0


Query: 19  DSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQ 78
           D G+C E+  +   +GGV D    QNS E+++LARFAV EHNKKEN LLEF RV+KAKEQ
Sbjct: 26  DLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQ 85

Query: 79  VVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 121
           VVAG L+HLTLE ++ G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 86  VVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKP 124

[Close]

BLAST of Carg28070-RA vs. TAIR10
Match: AT2G40880.1 (cystatin A)

HSP 1 Score: 131.3 bits (329), Expect = 3.9e-31
Identity = 62/89 (69.66%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 31  MKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLE 90
           M LGGV D  G QNS EI++LARFA+QEHNK++N +LEF++++KA+EQVVAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  AIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           A +G   K +EAKVWVKPWMNFKQLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Carg28070-RA vs. TAIR10
Match: AT5G05110.1 (Cystatin/monellin family protein)

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-30
Identity = 66/95 (69.47%), Postives = 73/95 (76.84%), Query Frame = 0


Query: 26  DPFIKMKLGGVRDYIGAQN-SLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKL 85
           D  +KMKLGG  D     N   EID +A FAVQEHN++EN +LE  RVLKA EQVVAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           Y LTLE I+ G KK+YEAKVWVKPWMNFKQLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Carg28070-RA vs. TAIR10
Match: AT5G12140.1 (cystatin-1)

HSP 1 Score: 102.1 bits (253), Expect = 2.6e-22
Identity = 50/86 (58.14%), Postives = 62/86 (72.09%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAI 92
           +GGVRD     N L++++LARFAV EHNK EN  LE++R+L AK QVVAG ++HLT+E  
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 93  DGGIKKVYEAKVWVKPWMNFKQLQEF 119
           DG   KVYEAKV  K W N KQL+ F
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESF 95

[Close]

BLAST of Carg28070-RA vs. TAIR10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 67.4 bits (163), Expect = 7.0e-12
Identity = 42/112 (37.50%), Postives = 61/112 (54.46%), Query Frame = 0


Query: 10  LLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEF 69
           L+++LLPL+ S           ++GG    I      ++  +  FAV E+NK+    L+F
Sbjct: 13  LVVVLLPLYASA--------AARVGGWSP-ISNVTDPQVVEIGEFAVSEYNKRSESGLKF 72

Query: 70  ERVLKAKEQVVAGKLYHLTLEAIDG-GIKKVYEAKVWVKPWMNFKQLQEFKP 121
           E V+  + QVV+G  Y L + A DG G+ K Y A VW KPWM F+ L  F+P
Sbjct: 73  ETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Carg28070-RA vs. Swiss-Prot
Match: sp|Q8H0X6|CYT6_ARATH (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 2.6e-32
Identity = 68/102 (66.67%), Postives = 82/102 (80.39%), Query Frame = 0


Query: 19  DSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQ 78
           D G+C E+  +   +GGV D    QNS E+++LARFAV EHNKKEN LLEF RV+KAKEQ
Sbjct: 26  DLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQ 85

Query: 79  VVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 121
           VVAG L+HLTLE ++ G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 86  VVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKP 124

[Close]

BLAST of Carg28070-RA vs. Swiss-Prot
Match: sp|Q06445|CYTI_VIGUN (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.4e-31
Identity = 65/87 (74.71%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAI 92
           LGG RD  G QNSLEID+LARFAV+EHNKK+N LLEF RV+ A++QVV+G LY +TLEA 
Sbjct: 4   LGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLEAK 63

Query: 93  DGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           DGG KKVYEAKVW KPW+NFK+LQEFK
Sbjct: 64  DGGQKKVYEAKVWEKPWLNFKELQEFK 90

[Close]

BLAST of Carg28070-RA vs. Swiss-Prot
Match: sp|Q0JNR2|CYT12_ORYSJ (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.2e-30
Identity = 63/87 (72.41%), Postives = 74/87 (85.06%), Query Frame = 0


Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAI 92
           LGG  D   A NS+E DALARFAV EHNK+EN LLEF RV++AKEQVVAG L+HLTLEA+
Sbjct: 48  LGGAHDAPSAANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEAL 107

Query: 93  DGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           + G KKVYEAKVWVKPW++FK+LQEF+
Sbjct: 108 EAGRKKVYEAKVWVKPWLDFKELQEFR 134

[Close]

BLAST of Carg28070-RA vs. Swiss-Prot
Match: sp|Q41906|CYT3_ARATH (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 7.1e-30
Identity = 62/89 (69.66%), Postives = 76/89 (85.39%), Query Frame = 0


Query: 31  MKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLE 90
           M LGGV D  G QNS EI++LARFA+QEHNK++N +LEF++++KA+EQVVAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  AIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           A +G   K +EAKVWVKPWMNFKQLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]

BLAST of Carg28070-RA vs. Swiss-Prot
Match: sp|Q8LC76|CYT7_ARATH (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 2.7e-29
Identity = 66/95 (69.47%), Postives = 73/95 (76.84%), Query Frame = 0


Query: 26  DPFIKMKLGGVRDYIGAQN-SLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKL 85
           D  +KMKLGG  D     N   EID +A FAVQEHN++EN +LE  RVLKA EQVVAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           Y LTLE I+ G KK+YEAKVWVKPWMNFKQLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Carg28070-RA vs. TrEMBL
Match: tr|A0A1S4E0R5|A0A1S4E0R5_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 7.1e-51
Identity = 101/121 (83.47%), Postives = 110/121 (90.91%), Query Frame = 0


Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ +EDPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
            KENGLLEF+RVLKAKEQVVAGKLYHLTLEAIDGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 C 122
           C
Sbjct: 121 C 121

[Close]

BLAST of Carg28070-RA vs. TrEMBL
Match: tr|A0FK05|A0FK05_POPTO (Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 6.9e-38
Identity = 84/114 (73.68%), Postives = 93/114 (81.58%), Query Frame = 0


Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCRQDNFLKMKLGGVHDCRGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLTLEA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTLEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]

BLAST of Carg28070-RA vs. TrEMBL
Match: tr|A9PCX4|A9PCX4_POPTR (Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-37
Identity = 83/114 (72.81%), Postives = 93/114 (81.58%), Query Frame = 0


Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCGQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLT+EA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTVEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]

BLAST of Carg28070-RA vs. TrEMBL
Match: tr|U5FQ48|U5FQ48_POPTR (Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 GN=POPTR_016G030900v3 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-37
Identity = 83/114 (72.81%), Postives = 93/114 (81.58%), Query Frame = 0


Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCGQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLT+EA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTVEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]

BLAST of Carg28070-RA vs. TrEMBL
Match: tr|A0FK03|A0FK03_POPTO (Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.0e-37
Identity = 83/114 (72.81%), Postives = 93/114 (81.58%), Query Frame = 0


Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCRQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +L+F RVLKAKEQVVAGKLYHLTLEA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILKFVRVLKAKEQVVAGKLYHLTLEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

The following BLAST results are available for this feature:

BLAST of Carg28070-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932782.1	9.3e-63	100.00	cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cystei...	[more]
XP_022967919.1	1.5e-60	96.69	cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine...	[more]
XP_023543498.1	9.6e-60	94.26	cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500....	[more]
XP_008455746.1	1.1e-50	83.47	PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDIC...	[more]
XP_011041371.1	2.7e-38	74.56	PREDICTED: cysteine proteinase inhibitor 6-like [Populus euphratica]	[more]

BLAST of Carg28070-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT3G12490.2	1.4e-33	66.67	cystatin B	[more]
AT2G40880.1	3.9e-31	69.66	cystatin A	[more]
AT5G05110.1	1.5e-30	69.47	Cystatin/monellin family protein	[more]
AT5G12140.1	2.6e-22	58.14	cystatin-1	[more]
AT5G47550.1	7.0e-12	37.50	Cystatin/monellin superfamily protein	[more]

BLAST of Carg28070-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8H0X6\|CYT6_ARATH	2.6e-32	66.67	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
sp\|Q06445\|CYTI_VIGUN	6.4e-31	74.71	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
sp\|Q0JNR2\|CYT12_ORYSJ	4.2e-30	72.41	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
sp\|Q41906\|CYT3_ARATH	7.1e-30	69.66	Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=...	[more]
sp\|Q8LC76\|CYT7_ARATH	2.7e-29	69.47	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]

BLAST of Carg28070-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4E0R5\|A0A1S4E0R5_CUCME	7.1e-51	83.47	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1	[more]
tr\|A0FK05\|A0FK05_POPTO	6.9e-38	73.68	Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=2 SV=1	[more]
tr\|A9PCX4\|A9PCX4_POPTR	1.5e-37	72.81	Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 PE=2 SV=1	[more]
tr\|U5FQ48\|U5FQ48_POPTR	1.5e-37	72.81	Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 GN=POPTR_016G030900...	[more]
tr\|A0FK03\|A0FK03_POPTO	2.0e-37	72.81	Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR018073	Prot_inh_cystat_CS
IPR027214	Cystatin
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg28070	Carg28070	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg28070-RA	Carg28070-RA-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg28070-RA.three_prime_UTR.2	Carg28070-RA.three_prime_UTR.2	three_prime_UTR
Carg28070-RA.three_prime_UTR.1	Carg28070-RA.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg28070-RA.CDS.2	Carg28070-RA.CDS.2	CDS
Carg28070-RA.CDS.1	Carg28070-RA.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg28070-RA.five_prime_UTR.1	Carg28070-RA.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg28070-RA.exon.3	Carg28070-RA.exon.3	exon
Carg28070-RA.exon.2	Carg28070-RA.exon.2	exon
Carg28070-RA.exon.1	Carg28070-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 31..121 e-value: 3.0E-27 score: 106.5
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 46..121 e-value: 3.1E-23 score: 81.8
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 44..120 e-value: 2.51986E-20 score: 79.4404
None	No IPR available	GENE3D	G3DSA:3.10.450.650		coord: 24..121 e-value: 1.9E-40 score: 140.2
None	No IPR available	PANTHER	PTHR11413:SF56	CYSTEINE PROTEINASE INHIBITOR 3	coord: 6..120
None	No IPR available	PRODOM	PD001231		coord: 45..121 e-value: 1.0E-29 score: 317.0
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 40..121
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 6..120
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 77..90