Carg28070 (gene) Silver-seed gourd

Overview
NameCarg28070
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCysteine proteinase inhibitor
LocationCucurbita_argyrosperma_scaffold_169 : 298571 .. 302319 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCAACCAACGATCCACACTAAAAAATTCACTCAATTTCTTCCTCCAATTATCAACTTCGCTCTTACAAAGTCCTGTGTTCCAATCCCAGAAAGCGACCATGAAAGTGAATCACATAGCTGCGGTGTTGCTGCTGCTTCTTCTTCCTCTGTTTGATTCGGGGTATTGCGTTGAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAACACAACAAGAAAGAGGTTTTTTCCTTTGTTTTCTTTGGTTTTTCAGCTCTTTATTCATTAATTTCTGGATTCGTTTCACTGCCTAATTTTCTTTCTTATTTTTGCTCAGAATCTAGTCACTTGCTTATTTAATTCGTTAATTAGTGGCTATTTGGTACCCATGTTTTGGTTGTTCATGCGTCGTTTTGATTTGTTAATCACCACTAGATGCGGTAGTTTAAATCGAATTCTTGACAGGCTTTTATGTCTCTTTGGTGCAGAATGGTCTTCTGGAATTTGAACGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCATTGATGGTGGTATAAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGATGGATCTTCAGGTGCCGTTCTGCTCCTCGTGTTCAAACTGGTATGGTAGGAATCGTTCTGTTCTATGTTAATTGTATATTTTGTATATCTGCATGACAATACTAGACTATTAGTCCATTTATGGTAGGAACCTAAGATAGCCTGTCTATGTCTGACACATTAGACACCTTTTTGGACTTTTTGTAACGGCCCAAGCCTACTGTTAGCAGATATTGTCCTCTTTAGGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAGAACGCGTTTGCTAGGGAAAGGTCTCCACACCCTTATAAAAGGTGTTTTGTTCTTCTCCCCAACCGATATGGGATCTCACACTTGTGATGGACACTTGTTAGTACAATAAATATATGTTATACAAAATCAATATAGGTCCAACATTTGTTAGACACGTACTGATCACTTGCTAAGTATATCAGTATAATAAAATGATAATAGAAAAATACATCACACATTGATTTAGTTATAAATGCATAAACTTTTTCGTTCTGAATTTTCTTATGCCATTGAAATGATATATATTTTAATAAATGTGTTCTTGTTGTGTACGTGTCCTAAATTTTTTAAAACATGTTGTGTCACTATGGCTGTGTCGTGTTTTGTACTTTGTATTTGTATTCGTGCTTCTTAGCTTATAATTTGGTATATGTACATGTGTAAATGTCAATAATTTGAGTTTTTGCTTTTGAAAACTTTTCTGAAAAGTTGAAGAAAGATAAAGGGTGTGAGACTAGTTAATCCTCTTTTTCTTATTCTTGTTTGTTATAATTGCTGCTGTGCTTGGTAGGATGATTGCAATGAGAGATCGATTCACTCTTGCTGAGCACCACTGCTCACAACAGAGAAAATGGCTCTCACACACTGTTATCAAAATAACTAACTATGTTAACGATACCAAAATGGTACGGAGGGATTAATGGTGAGTCCAAGTCAGTCCCAAACTAAATTGAATAAGAAATCTTTGTATCCTTAGTGTTGAATGTATTTGAAGCCAAATCAAGTGTTAGTATTGATAGATTTAAGTGATTTGGTTGTAACGGCCCAAGCTCACCGCTAGCAGATATTGTCCACTTTGGGCTTTTCTTTCTGGGATTCCCCTCAAGGTTTTTAAAACGCGTTTGCTAGGGAAAGGTTTTCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAACCGTTGTAGGATCTCACAATCTACCCCTCTTCGGGGTCCAGCGTCCTCACTAGCACTCGTTCTCTTCTCCAATCAATGTGGAACCTCCAATCTACCATTTTCGGGGCCCAGTGTCCTTGTTGGCACACCGCCTCATGTTCGCCCCCCTTTGGGGCTCAGTCTCCTTGCTGGCACTTTGTCCGGTGTTTGGCTCTGATACCATTGTAACGGTCCAAGTCCACCGCTAGCAGATATTTCCTCTTTGGGCTTTCCCTTTCGGGCTTTCCCTCAAGGTTTTTAAAACGCGTCTGCAAGGGAGAGATTTCTACACCCTTATAAAGAATACTTCGTTCTCCTCTCCAACAGATGTGGATCTCACATTAGTGTTTGAGTTTAATTAAGTCAAAGGGAAACAAGAATTTGGTGGGTTGTGTGGTCTCTCCATTTTTGTTATGTTATTTTGGTCTTCCATGGTCATCAGGAAGCATGAGAGATTTTAAAAAAATTTTCTTTGGATCATCTTAATGAATTTGATTAGTAAAAACACTTCCATTTGGCCAGTAGTTGTCTGCTAGTGTTCCCTGGAATCTAAGACTCTTTGGTATAAGATTATTGTTAGTTTGCATATCTTCTGGTTTTGAGGTCAGATCGATTTTTCTTTTGAGAATGGAGCTCGTGTTAACTTCTGGGGAGGATCGATTGATTCTAGTCGTTAACACCGTATATACCATCTAGAAGTTTATTATGTTCTTCTTCGTCTGGTTGTGTATTATCTATAAGGAGCAGATTAAATACTATAGGATTGTGTTTTGTGGATTTTATTTTTTTCAACATGGATTGGATATGGCTATAGCTCATCTTAAAGATTTTCTTGAGCAAGTAGTTGCAAGGATACCTCCTCATGGGAACTGCCACTTTAACAAACGACATGGAATATTTTAGAGGGGCAAAAATAAACATAGTATGACGTAGAATCTTATGCTGCGTCGTCTCAGTTTTATATATATATATATGCAAGCATTCAATTTATCAGTATATCATGTCAACTTGATCCCACATCGAAACACTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGCGTTATAAAATCGTGAAACTGACGGTGGTACATAACGAGCTAAATAGAACAATATCTGCTAGCAGTGGGCTTGGGCCGTTACAAATGGCAGTAGAGCTAGACACTGGGGATGTGCCAACAAAGATGTTGGGCCCCGAAGGGGGGTGAATTTCGTGGGGGTCTCGCATCGATTGGAGAAAGGAACGAGTGCCAACGAGGACGCTGGGCCTCGGAGGGGGGTGGATTGTGATATCCCACATTGGTTTGGGAGGACAACGAAACACCTTTTATAAGGGTGTGGAAACCTCTCCCTAACTTTGAGGGGAAGTGTGGAAGGGAAAGTTCACATAGGACAATATCGGCTAGTAGTGGGCTTGGACCGTTACACTTTCATTCTTAAATTGGTTTTTGTGTTACTGTCTAGCTAGAAATTTATCTTACAATAAATTGATATATTCATCTAGATGTTCAAATAAGGGCGATATGCATCAAAATAAGAACTATAGTTGTAATAATGTAACCAAAATTTATGTGTTATGTTTCGTGCATTGTTAAAATGGAGTCGAGAGTCGTATTGTGTCGTAAGATCGATTTCCGCTATGTTGATTGTTGTTCTTGGATGGCAGGTAAGTGAAGAGTATACATAAACATACCCTATAGCCCATGTGGGTTGCTGCTGAAGGTAAACAACAGAATAGATCTGAAACATTATAGGAACTCTGTTATATGATATGTTTATAATAATGGTGTAATATATATATATATATATATATATG

mRNA sequence

ATTTCAACCAACGATCCACACTAAAAAATTCACTCAATTTCTTCCTCCAATTATCAACTTCGCTCTTACAAAGTCCTGTGTTCCAATCCCAGAAAGCGACCATGAAAGTGAATCACATAGCTGCGGTGTTGCTGCTGCTTCTTCTTCCTCTGTTTGATTCGGGGTATTGCGTTGAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAACACAACAAGAAAGAGAATGGTCTTCTGGAATTTGAACGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCATTGATGGTGGTATAAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGATGGATCTTCAGGTGCCGTTCTGCTCCTCGTGTTCAAACTGGTAAGTGAAGAGTATACATAAACATACCCTATAGCCCATGTGGGTTGCTGCTGAAGGTAAACAACAGAATAGATCTGAAACATTATAGGAACTCTGTTATATGATATGTTTATAATAATGGTGTAATATATATATATATATATATATATG

Coding sequence (CDS)

ATGAAAGTGAATCACATAGCTGCGGTGTTGCTGCTGCTTCTTCTTCCTCTGTTTGATTCGGGGTATTGCGTTGAAGACCCATTTATCAAGATGAAACTGGGCGGTGTTCGCGATTACATCGGCGCTCAGAACAGCCTTGAAATTGACGCTCTCGCTCGTTTCGCAGTCCAAGAACACAACAAGAAAGAGAATGGTCTTCTGGAATTTGAACGGGTGTTGAAAGCTAAAGAACAGGTAGTTGCCGGTAAGTTGTATCATCTTACATTGGAAGCCATTGATGGTGGTATAAAAAAGGTTTATGAAGCCAAAGTGTGGGTGAAGCCATGGATGAACTTCAAACAGTTGCAGGAATTCAAACCATGCCCATGA

Protein sequence

MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKPCP
Homology
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BLAST of Carg28070 vs. NCBI nr
Match: XP_022932782.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932784.1 cysteine proteinase inhibitor A-like [Cucurbita moschata])

HSP 1 Score: 248.8 bits (634), Expect = 9.3e-63
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0 

Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
           KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 123
           CP
Sbjct: 121 CP 122

[Close]
BLAST of Carg28070 vs. NCBI nr
Match: XP_022967919.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967921.1 cysteine proteinase inhibitor A-like [Cucurbita maxima])

HSP 1 Score: 241.5 bits (615), Expect = 1.5e-60
Identity = 117/121 (96.69%), Postives = 121/121 (100.00%), Query Frame = 0 

Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHIAAVLL+LLLP+FDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIAAVLLVLLLPVFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
           KKENGLLEFERVLKAKEQVV+GKLYHLTLEAIDGG+KKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 C 122
           C
Sbjct: 121 C 121

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BLAST of Carg28070 vs. NCBI nr
Match: XP_023543498.1 (cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543501.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.8 bits (608), Expect = 9.6e-60
Identity = 115/122 (94.26%), Postives = 120/122 (98.36%), Query Frame = 0 

Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MKVNHI A+LLLLLLP+FDSGYCV+DPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN
Sbjct: 1   MKVNHIPALLLLLLLPVFDSGYCVQDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
           KK+NGLLEFERVLKAKEQVVAGKLYHLTLEA DGG+KKVYEAKVWVKPWMNFKQLQEFKP
Sbjct: 61  KKQNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKP 120

Query: 121 CP 123
           CP
Sbjct: 121 CP 122

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BLAST of Carg28070 vs. NCBI nr
Match: XP_008455746.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901819.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo])

HSP 1 Score: 208.8 bits (530), Expect = 1.1e-50
Identity = 101/121 (83.47%), Postives = 110/121 (90.91%), Query Frame = 0 

Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ +EDPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
            KENGLLEF+RVLKAKEQVVAGKLYHLTLEAIDGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 C 122
           C
Sbjct: 121 C 121

[Close]
BLAST of Carg28070 vs. NCBI nr
Match: XP_011041371.1 (PREDICTED: cysteine proteinase inhibitor 6-like [Populus euphratica])

HSP 1 Score: 167.5 bits (423), Expect = 2.7e-38
Identity = 85/114 (74.56%), Postives = 94/114 (82.46%), Query Frame = 0 

Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCRQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLTLEAID G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTLEAIDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]
BLAST of Carg28070 vs. TAIR10
Match: AT3G12490.2 (cystatin B)

HSP 1 Score: 139.4 bits (350), Expect = 1.4e-33
Identity = 68/102 (66.67%), Postives = 82/102 (80.39%), Query Frame = 0 

Query: 19  DSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQ 78
           D G+C E+  +   +GGV D    QNS E+++LARFAV EHNKKEN LLEF RV+KAKEQ
Sbjct: 26  DLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQ 85

Query: 79  VVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 121
           VVAG L+HLTLE ++ G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 86  VVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKP 124

[Close]
BLAST of Carg28070 vs. TAIR10
Match: AT2G40880.1 (cystatin A)

HSP 1 Score: 131.3 bits (329), Expect = 3.9e-31
Identity = 62/89 (69.66%), Postives = 76/89 (85.39%), Query Frame = 0 

Query: 31  MKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLE 90
           M LGGV D  G QNS EI++LARFA+QEHNK++N +LEF++++KA+EQVVAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  AIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           A +G   K +EAKVWVKPWMNFKQLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]
BLAST of Carg28070 vs. TAIR10
Match: AT5G05110.1 (Cystatin/monellin family protein)

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-30
Identity = 66/95 (69.47%), Postives = 73/95 (76.84%), Query Frame = 0 

Query: 26  DPFIKMKLGGVRDYIGAQN-SLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKL 85
           D  +KMKLGG  D     N   EID +A FAVQEHN++EN +LE  RVLKA EQVVAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           Y LTLE I+ G KK+YEAKVWVKPWMNFKQLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]
BLAST of Carg28070 vs. TAIR10
Match: AT5G12140.1 (cystatin-1)

HSP 1 Score: 102.1 bits (253), Expect = 2.6e-22
Identity = 50/86 (58.14%), Postives = 62/86 (72.09%), Query Frame = 0 

Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAI 92
           +GGVRD     N L++++LARFAV EHNK EN  LE++R+L AK QVVAG ++HLT+E  
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 93  DGGIKKVYEAKVWVKPWMNFKQLQEF 119
           DG   KVYEAKV  K W N KQL+ F
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESF 95

[Close]
BLAST of Carg28070 vs. TAIR10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 67.4 bits (163), Expect = 7.0e-12
Identity = 42/112 (37.50%), Postives = 61/112 (54.46%), Query Frame = 0 

Query: 10  LLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEF 69
           L+++LLPL+ S           ++GG    I      ++  +  FAV E+NK+    L+F
Sbjct: 13  LVVVLLPLYASA--------AARVGGWSP-ISNVTDPQVVEIGEFAVSEYNKRSESGLKF 72

Query: 70  ERVLKAKEQVVAGKLYHLTLEAIDG-GIKKVYEAKVWVKPWMNFKQLQEFKP 121
           E V+  + QVV+G  Y L + A DG G+ K Y A VW KPWM F+ L  F+P
Sbjct: 73  ETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]
BLAST of Carg28070 vs. Swiss-Prot
Match: sp|Q8H0X6|CYT6_ARATH (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 2.6e-32
Identity = 68/102 (66.67%), Postives = 82/102 (80.39%), Query Frame = 0 

Query: 19  DSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQ 78
           D G+C E+  +   +GGV D    QNS E+++LARFAV EHNKKEN LLEF RV+KAKEQ
Sbjct: 26  DLGFCNEEMAL---VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQ 85

Query: 79  VVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 121
           VVAG L+HLTLE ++ G KK+YEAKVWVKPW+NFK+LQEFKP
Sbjct: 86  VVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFKP 124

[Close]
BLAST of Carg28070 vs. Swiss-Prot
Match: sp|Q06445|CYTI_VIGUN (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.4e-31
Identity = 65/87 (74.71%), Postives = 75/87 (86.21%), Query Frame = 0 

Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAI 92
           LGG RD  G QNSLEID+LARFAV+EHNKK+N LLEF RV+ A++QVV+G LY +TLEA 
Sbjct: 4   LGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLEAK 63

Query: 93  DGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           DGG KKVYEAKVW KPW+NFK+LQEFK
Sbjct: 64  DGGQKKVYEAKVWEKPWLNFKELQEFK 90

[Close]
BLAST of Carg28070 vs. Swiss-Prot
Match: sp|Q0JNR2|CYT12_ORYSJ (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.2e-30
Identity = 63/87 (72.41%), Postives = 74/87 (85.06%), Query Frame = 0 

Query: 33  LGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLEAI 92
           LGG  D   A NS+E DALARFAV EHNK+EN LLEF RV++AKEQVVAG L+HLTLEA+
Sbjct: 48  LGGAHDAPSAANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEAL 107

Query: 93  DGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           + G KKVYEAKVWVKPW++FK+LQEF+
Sbjct: 108 EAGRKKVYEAKVWVKPWLDFKELQEFR 134

[Close]
BLAST of Carg28070 vs. Swiss-Prot
Match: sp|Q41906|CYT3_ARATH (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 7.1e-30
Identity = 62/89 (69.66%), Postives = 76/89 (85.39%), Query Frame = 0 

Query: 31  MKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKLYHLTLE 90
           M LGGV D  G QNS EI++LARFA+QEHNK++N +LEF++++KA+EQVVAG +YHLTLE
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQNKILEFKKIVKAREQVVAGTMYHLTLE 92

Query: 91  AIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           A +G   K +EAKVWVKPWMNFKQLQEFK
Sbjct: 93  AKEGDQTKNFEAKVWVKPWMNFKQLQEFK 121

[Close]
BLAST of Carg28070 vs. Swiss-Prot
Match: sp|Q8LC76|CYT7_ARATH (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 2.7e-29
Identity = 66/95 (69.47%), Postives = 73/95 (76.84%), Query Frame = 0 

Query: 26  DPFIKMKLGGVRDYIGAQN-SLEIDALARFAVQEHNKKENGLLEFERVLKAKEQVVAGKL 85
           D  +KMKLGG  D     N   EID +A FAVQEHN++EN +LE  RVLKA EQVVAGKL
Sbjct: 38  DNVVKMKLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKL 97

Query: 86  YHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           Y LTLE I+ G KK+YEAKVWVKPWMNFKQLQEFK
Sbjct: 98  YRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]
BLAST of Carg28070 vs. TrEMBL
Match: tr|A0A1S4E0R5|A0A1S4E0R5_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 7.1e-51
Identity = 101/121 (83.47%), Postives = 110/121 (90.91%), Query Frame = 0 

Query: 1   MKVNHIAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHN 60
           MK+NHIA  LL LLL  FD G+ +EDPFI+MKLGGVRDYIG+QN +EI++LARFAVQEHN
Sbjct: 1   MKLNHIAVPLLFLLLAAFDLGFSIEDPFIRMKLGGVRDYIGSQNRVEIESLARFAVQEHN 60

Query: 61  KKENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKP 120
            KENGLLEF+RVLKAKEQVVAGKLYHLTLEAIDGG KKVYEAKVWVKPWMNFKQLQEFK 
Sbjct: 61  NKENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKL 120

Query: 121 C 122
           C
Sbjct: 121 C 121

[Close]
BLAST of Carg28070 vs. TrEMBL
Match: tr|A0FK05|A0FK05_POPTO (Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 6.9e-38
Identity = 84/114 (73.68%), Postives = 93/114 (81.58%), Query Frame = 0 

Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCRQDNFLKMKLGGVHDCRGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLTLEA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTLEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]
BLAST of Carg28070 vs. TrEMBL
Match: tr|A9PCX4|A9PCX4_POPTR (Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-37
Identity = 83/114 (72.81%), Postives = 93/114 (81.58%), Query Frame = 0 

Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCGQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLT+EA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTVEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]
BLAST of Carg28070 vs. TrEMBL
Match: tr|U5FQ48|U5FQ48_POPTR (Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 GN=POPTR_016G030900v3 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-37
Identity = 83/114 (72.81%), Postives = 93/114 (81.58%), Query Frame = 0 

Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCGQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +LEF RVLKAKEQVVAGKLYHLT+EA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILEFVRVLKAKEQVVAGKLYHLTVEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

[Close]
BLAST of Carg28070 vs. TrEMBL
Match: tr|A0FK03|A0FK03_POPTO (Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.0e-37
Identity = 83/114 (72.81%), Postives = 93/114 (81.58%), Query Frame = 0 

Query: 6   IAAVLLLLLLPLFDSGYCVEDPFIKMKLGGVRDYIGAQNSLEIDALARFAVQEHNKKENG 65
           I +VL+LL     + G C +D F+KMKLGGV D  G+QNS EID+LARFAVQEHNKKEN 
Sbjct: 9   ILSVLVLLCGCYTELGLCRQDNFLKMKLGGVHDCKGSQNSAEIDSLARFAVQEHNKKENA 68

Query: 66  LLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFK 120
           +L+F RVLKAKEQVVAGKLYHLTLEA D G  K+YE KVWVKPWMNFKQLQEFK
Sbjct: 69  ILKFVRVLKAKEQVVAGKLYHLTLEATDAGNNKMYEVKVWVKPWMNFKQLQEFK 122

The following BLAST results are available for this feature:
BLAST of Carg28070 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022932782.19.3e-63100.00cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cystei... [more]
XP_022967919.11.5e-6096.69cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine... [more]
XP_023543498.19.6e-6094.26cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500.... [more]
XP_008455746.11.1e-5083.47PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDIC... [more]
XP_011041371.12.7e-3874.56PREDICTED: cysteine proteinase inhibitor 6-like [Populus euphratica][more]
BLAST of Carg28070 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G12490.21.4e-3366.67cystatin B[more]
AT2G40880.13.9e-3169.66cystatin A[more]
AT5G05110.11.5e-3069.47Cystatin/monellin family protein[more]
AT5G12140.12.6e-2258.14cystatin-1[more]
AT5G47550.17.0e-1237.50Cystatin/monellin superfamily protein[more]
BLAST of Carg28070 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8H0X6|CYT6_ARATH2.6e-3266.67Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=... [more]
sp|Q06445|CYTI_VIGUN6.4e-3174.71Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1[more]
sp|Q0JNR2|CYT12_ORYSJ4.2e-3072.41Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0... [more]
sp|Q41906|CYT3_ARATH7.1e-3069.66Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=... [more]
sp|Q8LC76|CYT7_ARATH2.7e-2969.47Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=... [more]
BLAST of Carg28070 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S4E0R5|A0A1S4E0R5_CUCME7.1e-5183.47Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1[more]
tr|A0FK05|A0FK05_POPTO6.9e-3873.68Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=2 SV=1[more]
tr|A9PCX4|A9PCX4_POPTR1.5e-3772.81Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 PE=2 SV=1[more]
tr|U5FQ48|U5FQ48_POPTR1.5e-3772.81Cysteine proteinase inhibitor OS=Populus trichocarpa OX=3694 GN=POPTR_016G030900... [more]
tr|A0FK03|A0FK03_POPTO2.0e-3772.81Cysteine proteinase inhibitor OS=Populus tomentosa OX=118781 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR018073Prot_inh_cystat_CS
IPR027214Cystatin
IPR000010Cystatin_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg28070-RACarg28070-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 31..121
e-value: 3.0E-27
score: 106.5
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 46..121
e-value: 3.1E-23
score: 81.8
IPR000010Cystatin domainCDDcd00042CYcoord: 44..120
e-value: 2.51986E-20
score: 79.4404
NoneNo IPR availableGENE3DG3DSA:3.10.450.650coord: 24..121
e-value: 1.9E-40
score: 140.2
NoneNo IPR availablePANTHERPTHR11413:SF56CYSTEINE PROTEINASE INHIBITOR 3coord: 6..120
NoneNo IPR availablePRODOMPD001231coord: 45..121
e-value: 1.0E-29
score: 317.0
NoneNo IPR availableSUPERFAMILYSSF54403Cystatin/monellincoord: 40..121
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 6..120
IPR018073Proteinase inhibitor I25, cystatin, conserved sitePROSITEPS00287CYSTATINcoord: 77..90

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg28070CmaCh03G001560Cucurbita maxima (Rimu)carcmaB1225
Carg28070CmoCh03G010910Cucurbita moschata (Rifu)carcmoB1195
Carg28070Cp4.1LG10g10610Cucurbita pepo (Zucchini)carcpeB1234
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None