Carg23580-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23580-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Secretory carrier-associated membrane protein
Location	Cucurbita_argyrosperma_scaffold_235 : 146573 .. 149884 (-)
Sequence length	429

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCGCCACAATGATCCAAATCCGTTCGATGAAGGAGAGGTCAATCCATTCTCGGTAAGTTTCTCTTTCTTTCGTGGCTAAATCTCATTGTTTTTTTTTTTTTCGCATTTTCGTTTCTTGTTTCGGCGATTTTCATTCGGCGAGTGTCGCGATCGGGTTCTGTTCCTATTTTTGAGTCTCGAGGGGGCGAGAGTGAGGTTGTTGGATTGATCTGAATCTTAGGGAAATCTTGCTAAATTTGGTGGAATACGGTTAGTTTATGGCTGCTGAGAAATTTCGTGGGAGACTGAACAAGGGCGGTGTGGAACATTTGAAGTGTTGAAAATCATGGGCTCCCTAATGGTATGATATCAATGGAGATGCATTCCTTACTTATAAACTCAAGATCGACCCTCCCCATAATGGTATGATGTCAATGGAGATGCATTCCTTACTTATAAACTCAAGATCGACCCTCTCCATAATGGTATGATATCAATGGAGATGCATTCCTTACTTATAAACTCAAGATCGACCCTCTCCATAATGGTGACCATCTCCATAATGGTATGATATCAATGGAGATGCATTTCTTACTTATAAACTCAAGATCGACCATCTCCATAATGGTATGGTGTCAATGGAGATGCATTTCTTACTTATAAACTCAAGATCGACCCTCCCCATAATGGTGTGATGTCAATGGAGATGCATTCCTTACTTATAAACTCAAGATCGACCCTCTCCATAATGGTATGATGTCAATGGAGATGCATTCCTTAATAATAAACTCAAGATTGACCCTCTCCATAATGGTATGATATCAATGGAGATGCATTCCTTACTTATAAACTCAAGATCGACCATCTCCATAATGGTGTGAGTTCAATGGAGATGCATTCCTTACTTATAAACTCAAGATTGACCATCTCCATAATGGTATGATGTCAATGGAGATGCTTTCCTTACTTATAAACTCAAGATCGACCCTCTCCATAATGGTATGATGTCAATGGAGATGCATTCCTTACTTATAAACTCAGGATCGACCCTCTCCATAATGGTATGATATCAATGGAAATGGAGATGTATTCCTTACTTATAAACCCAGGATCAACCTCTTAATTAGCCGATGTGGGACTCCTCTTCCAACAATCCTCCCCTTGAACTCGAACAAAGTACACCATAGAGCCTCCCTTGAGGTCTATGGAGCCCTCGAACAACCTCCCCTTAATCGAGGCTTGACTCCTTCTCTGGAGCTCTTGAACAAAGTACACCTTCTGTTCGACACTTGAGTCACTTTTGACTACACTTTTGAGGGTCTCAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGGCTAAGTTAAGGGCATGACTCTGATACCATGTTAGAAGTTGGTAAAATCACAACTCTTCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGACTTTGTTTTTAACTTCCCCAAAAGGCCTCGTACCAATGGAGATGTATTTCTTACTTATAAACCCACTATCAACCCCTTAATTAGCTGCCGACGTGGACTCCTCTTCCAACCATCCTCAACAGTTTGTTTAAGGATGGAGTAGAAATTAATGTTTAAAGGTTGAACGGCACTAAAAGTAGACTTACGTTTTGGAGTCCTAAGGAAGATGATTTCACCATTTCGGATTGAGAATGAGTGTCATTACAATTCTGTGTGGAATTGTTATTTGAATATGCATAAATGAAATCAACCGTTTGTTGATTATTGACAATTTCACAGGCGTTCTTTGATATCTGAAACTAAAAACAAGCAAAATCAAGCTTGTTCTATGGAGGATTGGAATGGTAATAACATCACTTGTTGCTAATTTTGACATTTGCAGAAAGACAATGCTGCTCCTGGATCTAAGTTTCGGATCCCACAAATGATATCGGACACTCTGGGATTTGGGCAGAAGCAGGATGCTACAGTGGATATACCACTGGATACGATGAATGTAACGAATATCATGTCCTGTCTTTTCTTGCTTACATTTTCTTTACCTAGAAGAATCAAGTAACGCTTCATTTGGTTCGTGTAGAATCTGAGGAAGAATGAGAGAGACATTGCCGCTTGGGAAGCAGAACTTAATAGGAAAGAAAAGGTATTCTTCGATATCCATGATTTTGAACTTGACAATATATGAATATATCTGTAGAGGTTACCTAACTGTTCTTATGCCTCATTTTTACCAGGAAATCAAAAAGAGGGAAGAAGCTGTTTCTAAAGGTGGTTGACATCAAATGTTGTTTGATTTTGTTTTTATGAAGCTATCGACAATCTATAACTTTTTCAATGTTGCACAGCTGGTGTCCCCACTGATGATAGAAACTGGCCTCCATTTTTCCCCATTATTCATCATGATATAGCTAATGAAATTCCAGTACATGCTCAGAAGTTGCAGTACTTGGCTTTCGCTAGCTGGCTAGGTATGCAAAGTTAATTGTCTCAATTCTTTTTTGTATAAACTTTATATCCAAATATTCGAGTATAAATTGTGAGATCCTACATCGGTTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTGACAACCTCTCTCTAGTAGACACGTTTTAAAAACCTTGAGGGGAAGCCCAAAGAGGACAATATCTGTTAGCGGTAGGCTTGAGCGGTTACAAACACATCGATTGGAGAGGGGAACGAGTGCCAGCAAGGACATTGGACTTGGGGGGGGGGGGGGGGAATTGTGAGATCCCACGTCGGTTGGAGAGGGGCATGAAGCATTCTTTACAAGAGTATGAAAACCTCTCCTTAGTAGACGCGTTTTAAAACTTTGAGGGGAAGCCCGGAAGGGAAAGTCCAAAGAAGACAATATCTACTGGCGGTGGGCTTGAGCTGTTACACAAACATTCTTGAAAGTGTTGCTTTTCTATGTGTATATGATGTTCTTCACCTCACTTAAGTTCTTCTTGCCTCACTTAGCGTCTCGTCCCCGACATTATCTTTTCAAACCATATGAACGTAGTTATTTTTAACTCTACATTTTATCCCTTGCTGTTTTTGCAGGCATAGGGTTGTGCTTGGTCTTCAATGTTATTGCTATAACTGTCTGCTGGATAAGAGGCGGGGGTAATTACTTCACTATCAGTTTTCTATTTTTTGTGCAATGGGTCACTTTAAGCTGCAACAAATGGATTTTTTTTTTACTGTTGTCACAACTTGCAGGAGTCACAATCTTTTTACTTGCTGTAATATATGCCATACTTGGAATTCCTCTTTCATATGTGCTATGGTATAGGCCTCTATACCGTGCTATGAGGTGA

mRNA sequence

ATGAATCGCCACAATGATCCAAATCCGTTCGATGAAGGAGAGGTCAATCCATTCTCGAAAGACAATGCTGCTCCTGGATCTAAGTTTCGGATCCCACAAATGATATCGGACACTCTGGGATTTGGGCAGAAGCAGGATGCTACAGTGGATATACCACTGGATACGATGAATAATCTGAGGAAGAATGAGAGAGACATTGCCGCTTGGGAAGCAGAACTTAATAGGAAAGAAAAGGAAATCAAAAAGAGGGAAGAAGCTGTTTCTAAAGGCATAGGGTTGTGCTTGGTCTTCAATGTTATTGCTATAACTGTCTGCTGGATAAGAGGCGGGGGAGTCACAATCTTTTTACTTGCTGTAATATATGCCATACTTGGAATTCCTCTTTCATATGTGCTATGGTATAGGCCTCTATACCGTGCTATGAGGTGA

Coding sequence (CDS)

ATGAATCGCCACAATGATCCAAATCCGTTCGATGAAGGAGAGGTCAATCCATTCTCGAAAGACAATGCTGCTCCTGGATCTAAGTTTCGGATCCCACAAATGATATCGGACACTCTGGGATTTGGGCAGAAGCAGGATGCTACAGTGGATATACCACTGGATACGATGAATAATCTGAGGAAGAATGAGAGAGACATTGCCGCTTGGGAAGCAGAACTTAATAGGAAAGAAAAGGAAATCAAAAAGAGGGAAGAAGCTGTTTCTAAAGGCATAGGGTTGTGCTTGGTCTTCAATGTTATTGCTATAACTGTCTGCTGGATAAGAGGCGGGGGAGTCACAATCTTTTTACTTGCTGTAATATATGCCATACTTGGAATTCCTCTTTCATATGTGCTATGGTATAGGCCTCTATACCGTGCTATGAGGTGA

Protein sequence

MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLRKNERDIAAWEAELNRKEKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYRAMR

Homology

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BLAST of Carg23580-RA vs. NCBI nr
Match: XP_022923600.1 (secretory carrier-associated membrane protein 4-like [Cucurbita moschata])

HSP 1 Score: 261.5 bits (667), Expect = 1.6e-66
Identity = 142/183 (77.60%), Postives = 142/183 (77.60%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR
Sbjct: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60

Query: 61  KNERDIAAWEAELNRKEKEIKKREEAVSK------------------------------- 120
           KNERDIAAWEAELNRKEKEIKKREEAVSK                               
Sbjct: 61  KNERDIAAWEAELNRKEKEIKKREEAVSKAGVPTDDRNWPPFFPIIHHDIANEIPVHAQK 120

Query: 121 ----------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 143
                     GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR
Sbjct: 121 LQYLAFASWLGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 180

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BLAST of Carg23580-RA vs. NCBI nr
Match: XP_022965309.1 (secretory carrier-associated membrane protein 4-like [Cucurbita maxima])

HSP 1 Score: 260.4 bits (664), Expect = 3.6e-66
Identity = 141/183 (77.05%), Postives = 142/183 (77.60%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR
Sbjct: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60

Query: 61  KNERDIAAWEAELNRKEKEIKKREEAVSK------------------------------- 120
           KNERDIAAWEAELNRKEKEIKKREEAVS+                               
Sbjct: 61  KNERDIAAWEAELNRKEKEIKKREEAVSRAGVPTDDRNWPPFFPIIHHDIANEIPVHAQK 120

Query: 121 ----------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 143
                     GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR
Sbjct: 121 LQYLAFASWLGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 180

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BLAST of Carg23580-RA vs. NCBI nr
Match: XP_023552124.1 (secretory carrier-associated membrane protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259.2 bits (661), Expect = 8.0e-66
Identity = 140/183 (76.50%), Postives = 142/183 (77.60%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMI+DTLGFGQKQDATVDIPLDTMNNLR
Sbjct: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMITDTLGFGQKQDATVDIPLDTMNNLR 60

Query: 61  KNERDIAAWEAELNRKEKEIKKREEAVSK------------------------------- 120
           KNERDIAAWEAELNRKEKEIKKREEAVS+                               
Sbjct: 61  KNERDIAAWEAELNRKEKEIKKREEAVSRAGVPTDDRNWPPFFPIIHHDIANEIPVHAQK 120

Query: 121 ----------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 143
                     GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR
Sbjct: 121 LQYLAFASWLGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 180

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BLAST of Carg23580-RA vs. NCBI nr
Match: XP_017234947.1 (PREDICTED: secretory carrier-associated membrane protein 4 isoform X2 [Daucus carota subsp. sativus])

HSP 1 Score: 199.9 bits (507), Expect = 5.8e-48
Identity = 95/142 (66.90%), Postives = 120/142 (84.51%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MNR +D NPFDE  VNPFS  NA PGSK RIP+M+++TLGFGQK DA VDIPLD+M + +
Sbjct: 1   MNRRDDQNPFDEEAVNPFSNGNADPGSKSRIPKMVANTLGFGQKNDANVDIPLDSMKDPK 60

Query: 61  KNERDIAAWEAELNRKEKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVI 120
           K E+++AAW+AEL ++E+++K+RE+AV+ GI +CLVFN+IA+TVCWI+ GGV IF LA I
Sbjct: 61  KKEKELAAWDAELKKRERDLKRREDAVAGGINICLVFNLIAVTVCWIKHGGVKIFFLATI 120

Query: 121 YAILGIPLSYVLWYRPLYRAMR 143
           Y +LG PLSYVLWYRPLYRAMR
Sbjct: 121 YMLLGCPLSYVLWYRPLYRAMR 142

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BLAST of Carg23580-RA vs. NCBI nr
Match: XP_011074780.1 (secretory carrier-associated membrane protein 4 [Sesamum indicum])

HSP 1 Score: 197.6 bits (501), Expect = 2.9e-47
Identity = 107/185 (57.84%), Postives = 126/185 (68.11%), Query Frame = 0


Query: 1   MNRHNDPNPFDEG--EVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNN 60
           M R NDPNPFDEG  E+NPFS D AA GSK RIP++++ TLGFGQK DA VDIPLDTMN+
Sbjct: 1   MGRRNDPNPFDEGEPEINPFSNDTAAAGSKSRIPKVVASTLGFGQKNDANVDIPLDTMND 60

Query: 61  LRKNERDIAAWEAELNRKEKEIKKREEAVSK----------------------------- 120
            RK E+++A WE++LNR+E+EIK+RE+AV++                             
Sbjct: 61  PRKKEKELATWESDLNRREREIKRREDAVARAGVPVDDKNWPPCFPIIHHDIANEIPVPA 120

Query: 121 ------------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPL 143
                       GI LCLVFNVIAITVCWIRGGGV IF LA+IYA+LG PLSYVLWYRPL
Sbjct: 121 QRLQYLAFASWLGILLCLVFNVIAITVCWIRGGGVKIFFLAIIYALLGCPLSYVLWYRPL 180

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BLAST of Carg23580-RA vs. TAIR10
Match: AT1G32050.1 (SCAMP family protein)

HSP 1 Score: 153.3 bits (386), Expect = 1.1e-37
Identity = 93/185 (50.27%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGE--VNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNN 60
           MNRH+DPNPFDE E  VNPFSK         R+P   S  + +GQ  DATVDIPLD MN+
Sbjct: 1   MNRHHDPNPFDEDEEIVNPFSKGGG------RVP-AASRPVEYGQSLDATVDIPLDNMND 60

Query: 61  LRKNERDIAAWEAELNRKEKEIKKREEAVSK----------------------------- 120
             + +R +A WEAEL +KE +IK+REEA++K                             
Sbjct: 61  SSQKQRKLADWEAELRKKEMDIKRREEAIAKFGVQIDDKNWPPFFPIIHHDIAKEIPVHA 120

Query: 121 ------------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPL 143
                       GI LCLVFNVIA  VCWI+GGGV IF LA IYA++G PLSYVLWYRPL
Sbjct: 121 QKLQYLAFASWLGIVLCLVFNVIATMVCWIKGGGVKIFFLATIYALIGCPLSYVLWYRPL 178

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BLAST of Carg23580-RA vs. TAIR10
Match: AT1G61250.1 (secretory carrier 3)

HSP 1 Score: 73.9 bits (180), Expect = 8.7e-14
Identity = 35/53 (66.04%), Postives = 43/53 (81.13%), Query Frame = 0


Query: 90  GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYRAMR 143
           G+ LCL +N+IA+T  WI+G GVTI+LLA+IY I G+P  YVLWYRPLYRA R
Sbjct: 133 GLVLCLFWNIIAVTTAWIKGEGVTIWLLALIYFIAGVPGGYVLWYRPLYRAFR 185

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BLAST of Carg23580-RA vs. TAIR10
Match: AT1G03550.1 (Secretory carrier membrane protein (SCAMP) family protein)

HSP 1 Score: 72.0 bits (175), Expect = 3.3e-13
Identity = 64/184 (34.78%), Postives = 88/184 (47.83%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVN-PFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNL 60
           M RH DPNPF + E+N   +  +  P S   +  +  +    G    ATVDIPLD+ N+L
Sbjct: 1   MARH-DPNPFADEEINXXXNHTSVPPASNSYLKPLPPEPYDRG----ATVDIPLDSGNDL 60

Query: 61  RKNERDIAAWE------------------------AELNRKE----------KEI----- 120
           R  E                                E N  E           EI     
Sbjct: 61  RAKEMXXXXXXXXXXXXXXXXXXXXXXXXXXGVVIEEKNWPEFFPLIHHDIPNEIPIHLQ 120

Query: 121 KKREEAVSKGIGL--CLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLY 143
           K +  A +  +GL  CL++N++A+TV WI+G G TI+LL++IY + G+P +YVLWYRPLY
Sbjct: 121 KIQYVAFTTLLGLVGCLLWNIVAVTVAWIKGEGPTIWLLSIIYFLAGVPGAYVLWYRPLY 179

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BLAST of Carg23580-RA vs. TAIR10
Match: AT2G20840.1 (Secretory carrier membrane protein (SCAMP) family protein)

HSP 1 Score: 72.0 bits (175), Expect = 3.3e-13
Identity = 56/186 (30.11%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           M+R+   + FD+GE+NPF+   + P +  ++  +  +    G    AT+DIPLD+  +L+
Sbjct: 1   MSRYQS-HSFDDGEINPFANPTSVPAATSKLSPLPPEPYDRG----ATMDIPLDSGKDLK 60

Query: 61  ------------------------------------KN--------ERDIAAWEAELNRK 120
                                               KN          DI+    E+   
Sbjct: 61  AKXXXXXXXXXXXXXXXXXXXXXXXXXXXAGIVIEEKNWPPFFPLIHHDIS---NEIPIH 120

Query: 121 EKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRP 143
            + I+        G+ +CL++N++A+T  WI+G G TI+ LA+IY I G+P +YV+WYRP
Sbjct: 121 LQRIQYVAFTSMLGLVVCLLWNIVAVTTAWIKGEGPTIWFLAIIYFISGVPGAYVMWYRP 178

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BLAST of Carg23580-RA vs. TAIR10
Match: AT1G11180.2 (Secretory carrier membrane protein (SCAMP) family protein)

HSP 1 Score: 70.1 bits (170), Expect = 1.3e-12
Identity = 65/202 (32.18%), Postives = 87/202 (43.07%), Query Frame = 0


Query: 1   MNRHNDPNPFDE-GEVNPFSKDNAAP-GSKFRIPQMISDTLGFGQKQDATVDIPLDT--- 60
           M    D N FDE  EVNPF+   + P  S  R+  +  +  GFG  +  TVDIPLD    
Sbjct: 1   MGGRYDRNTFDEQDEVNPFANPGSVPAASNSRLSPLPPEPAGFGYGR--TVDIPLDRPGS 60

Query: 61  --------------MNNLRKNER------------------------------DIAAWEA 120
                         + +L+K E                               +   W  
Sbjct: 61  GAQNNSCLNVTLLGIQDLKKKEXXXXXXXXXXXXXXXXXXXXXXXAARAGIVIEAKNWPT 120

Query: 121 -----------ELNRKEKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVI 143
                      E+  + + ++    A   G+ L L +N+IA+T  WI+G GVTI+LLAVI
Sbjct: 121 FFPLIHHDIANEILVRLQRLQYIAFATYLGLVLALFWNIIAVTTAWIKGEGVTIWLLAVI 180

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BLAST of Carg23580-RA vs. Swiss-Prot
Match: sp|Q9C6X2|SCAM4_ARATH (Secretory carrier-associated membrane protein 4 OS=Arabidopsis thaliana OX=3702 GN=SCAMP4 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.0e-36
Identity = 93/185 (50.27%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGE--VNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNN 60
           MNRH+DPNPFDE E  VNPFSK         R+P   S  + +GQ  DATVDIPLD MN+
Sbjct: 1   MNRHHDPNPFDEDEEIVNPFSKGGG------RVP-AASRPVEYGQSLDATVDIPLDNMND 60

Query: 61  LRKNERDIAAWEAELNRKEKEIKKREEAVSK----------------------------- 120
             + +R +A WEAEL +KE +IK+REEA++K                             
Sbjct: 61  SSQKQRKLADWEAELRKKEMDIKRREEAIAKFGVQIDDKNWPPFFPIIHHDIAKEIPVHA 120

Query: 121 ------------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPL 143
                       GI LCLVFNVIA  VCWI+GGGV IF LA IYA++G PLSYVLWYRPL
Sbjct: 121 QKLQYLAFASWLGIVLCLVFNVIATMVCWIKGGGVKIFFLATIYALIGCPLSYVLWYRPL 178

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BLAST of Carg23580-RA vs. Swiss-Prot
Match: sp|Q0JAI9|SCAM6_ORYSJ (Secretory carrier-associated membrane protein 6 OS=Oryza sativa subsp. japonica OX=39947 GN=SCAMP6 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.3e-19
Identity = 67/186 (36.02%), Postives = 90/186 (48.39%), Query Frame = 0


Query: 4   HNDPNPFDEGEV--NPFSKDNAAPGSKFRIPQ---MISDTLGFGQ-KQDATVDIPLDTMN 63
           H+DPNPFDEG    NPFS                   ++  GFG  + DATVD+PLDTM 
Sbjct: 2   HHDPNPFDEGNADDNPFSXXXXXXXXXXXXXXYGFRPTEPAGFGAGRGDATVDVPLDTMG 61

Query: 64  NLRKNERDIAAWEA-----------------------------------------ELNRK 123
           + +   R++++WE                                          E+   
Sbjct: 62  DSKSKARELSSWETXXXXXXXXXXXXXXXXXNAGVPMEDKNWPPFFPIIHHDIANEIPAN 121

Query: 124 EKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRP 143
            ++++    A   GI LCL +N IA+ VCWI+ G   +F LA IYA+LGIPLSY++WYRP
Sbjct: 122 LQKLQYLAFASWLGIVLCLSWNFIAVIVCWIKEGDSKLFFLATIYALLGIPLSYLIWYRP 181

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BLAST of Carg23580-RA vs. Swiss-Prot
Match: sp|Q6Z8F5|SCAM5_ORYSJ (Secretory carrier-associated membrane protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=SCAMP5 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 8.9e-16
Identity = 63/194 (32.47%), Postives = 84/194 (43.30%), Query Frame = 0


Query: 4   HNDPNPFDEG-EVNPFSKDNAAPGSKFRIPQMISDTLGFGQK-------------QDATV 63
           H+DPNPFDEG + NPFS                        +               ATV
Sbjct: 2   HHDPNPFDEGADDNPFSXXXXXXXXXXXXXXXXXXXXXXXSQFSFXXXXXXXXXXXXATV 61

Query: 64  DIPLDTMNNLRKNERDIAAWEA-------------------------------------- 123
           DIPLD M++ +   +++  WEA                                      
Sbjct: 62  DIPLDNMSDSKGKGKELLQWEADXXXXXXXXXXXXXXXXSAGVPMEEKNWPPFFPIIHHD 121

Query: 124 ---ELNRKEKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPL 143
              E+    ++++    A   GI LCL +N IA+ VCWIRGG   +F LA IY +LG+PL
Sbjct: 122 IANEIPANAQKLQYLAFASWLGIVLCLFWNFIAVIVCWIRGGDSKLFFLATIYGMLGMPL 181

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BLAST of Carg23580-RA vs. Swiss-Prot
Match: sp|Q9ZTX0|SCAM_PEA (Secretory carrier-associated membrane protein OS=Pisum sativum OX=3888 GN=PSAM2 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.7e-14
Identity = 60/179 (33.52%), Postives = 82/179 (45.81%), Query Frame = 0


Query: 6   DPNPFDEGEVNPFSKD-NAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLRKNER 65
           DPNPFDE +VNPFS   +AA  +  R   +  D   +      TVDIPLDT  + +K ER
Sbjct: 6   DPNPFDEEQVNPFSNPRSAASATNSRPAPLNPDRADYNYGFGPTVDIPLDTSTDGKKKER 65

Query: 66  ------------------------------DIAAWEA-----------ELNRKEKEIKKR 125
                                         +   W             E+    + ++  
Sbjct: 66  XXXXXXXXXXXXXXXXXXXXXXXXXXGIVIEEKNWPPFFPIIHHDITNEIPIHLRTLQYV 125

Query: 126 EEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYRAMR 143
                 G+ LCL +NV+++T  WI+G GV I+ LA+IY I G+P +Y LWYRPLYRA R
Sbjct: 126 AFFSLLGLVLCLTWNVVSVTAAWIKGEGVKIWFLAIIYFIAGVPGAYALWYRPLYRAFR 184

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BLAST of Carg23580-RA vs. Swiss-Prot
Match: sp|Q9SXA5|SCAM5_ARATH (Secretory carrier-associated membrane protein 5 OS=Arabidopsis thaliana OX=3702 GN=SCAMP5 PE=2 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.2e-12
Identity = 65/189 (34.39%), Postives = 86/189 (45.50%), Query Frame = 0


Query: 1   MNRHNDPNPFDE-GEVNPFSKDNAAP-GSKFRIPQMISDTLGFGQKQDATVDIPLDT--- 60
           M    D N FDE  EVNPF+   + P  S  R+  +  +  GFG  +  TVDIPLD    
Sbjct: 1   MGGRYDRNTFDEQDEVNPFANPGSVPAASNSRLSPLPPEPAGFGYGR--TVDIPLDRPGS 60

Query: 61  -MNNLRKNER------------------------------DIAAWEA-----------EL 120
              +L+K E                               +   W             E+
Sbjct: 61  GAQDLKKKEXXXXXXXXXXXXXXXXXXXXXXXAARAGIVIEAKNWPTFFPLIHHDIANEI 120

Query: 121 NRKEKEIKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLW 143
             + + ++    A   G+ L L +N+IA+T  WI+G GVTI+LLAVIY I G+P  YVLW
Sbjct: 121 LVRLQRLQYIAFATYLGLVLALFWNIIAVTTAWIKGEGVTIWLLAVIYFISGVPGGYVLW 180

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BLAST of Carg23580-RA vs. TrEMBL
Match: tr|A0A2R6PHR2|A0A2R6PHR2_ACTCH (Secretory carrier-associated membrane protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc28733 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.5e-47
Identity = 106/183 (57.92%), Postives = 126/183 (68.85%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MN  NDPNPFDE EVNPFSK ++APGSK RIPQM++ TLGFGQK DATVDIPLDTMN+ +
Sbjct: 1   MNSRNDPNPFDE-EVNPFSKGSSAPGSKSRIPQMVAQTLGFGQKHDATVDIPLDTMNDGK 60

Query: 61  KNERDIAAWEAELNRKEKEIKKREEAVSK------------------------------- 120
             E+++AAW+A+L R+E+EIK+RE+AV+                                
Sbjct: 61  SKEKELAAWDADLKRREREIKRREDAVASAGVPADDRNWPPFFPIIHHDIANEIPVHAQK 120

Query: 121 ----------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 143
                     GI LCLVFNV+A+TVCWIRGGGV IF LA+IYA+LG PLSYVLWYRPLYR
Sbjct: 121 LQYLAFASWLGIVLCLVFNVVAVTVCWIRGGGVKIFFLAIIYALLGCPLSYVLWYRPLYR 180

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BLAST of Carg23580-RA vs. TrEMBL
Match: tr|A0A1S3BK39|A0A1S3BK39_CUCME (Secretory carrier-associated membrane protein OS=Cucumis melo OX=3656 GN=LOC103490740 PE=3 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.6e-46
Identity = 110/183 (60.11%), Postives = 120/183 (65.57%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MNRHNDPNPFDE EVNPFSK   APGSKFRIPQM+SDTLGFGQK DATVDIPLDTMN+ +
Sbjct: 1   MNRHNDPNPFDEEEVNPFSKGGGAPGSKFRIPQMVSDTLGFGQKHDATVDIPLDTMNDPK 60

Query: 61  KNERDIAAWEA-----------------------------------------ELNRKEKE 120
           K ER++AAWEA                                         E+    ++
Sbjct: 61  KKERELAAWEAXXXXXXXXXXXXXXXXSKAGVPADDRNWPPFFPIIHHDIANEIPVHAQK 120

Query: 121 IKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 143
           ++    A   GI LCL FNVIAITVCWIRGGGV IF LAVIYAILGIPLSYVLWYRPLYR
Sbjct: 121 LQYLAFASWLGIVLCLFFNVIAITVCWIRGGGVKIFFLAVIYAILGIPLSYVLWYRPLYR 180

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BLAST of Carg23580-RA vs. TrEMBL
Match: tr|A0A0A0L3U1|A0A0A0L3U1_CUCSA (Secretory carrier-associated membrane protein OS=Cucumis sativus OX=3659 GN=Csa_3G006700 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.5e-44
Identity = 107/183 (58.47%), Postives = 118/183 (64.48%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNNLR 60
           MNRHNDPNPFDE  VNPFSK   APGSKFRIPQM+S+TLGFGQK DATVDIPLDTMN+ +
Sbjct: 1   MNRHNDPNPFDEEGVNPFSKGGGAPGSKFRIPQMVSETLGFGQKHDATVDIPLDTMNDPK 60

Query: 61  KNERDIAAWEA-----------------------------------------ELNRKEKE 120
           K ER++AAW                                           E+    ++
Sbjct: 61  KKERELAAWXXXXXXXXXXXXXXXXXXXXAGVPADDRNWPPFFPIIHHDIANEIPVHAQK 120

Query: 121 IKKREEAVSKGIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPLYR 143
           ++    A   GI LCLVFNVIAITVCWIRGGGV IF LAVIYAILGIPLSYVLWYRPLYR
Sbjct: 121 LQYLAFASWLGIVLCLVFNVIAITVCWIRGGGVKIFFLAVIYAILGIPLSYVLWYRPLYR 180

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BLAST of Carg23580-RA vs. TrEMBL
Match: tr|A0A2G9FZP0|A0A2G9FZP0_9LAMI (Secretory carrier-associated membrane protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_28982 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 7.5e-44
Identity = 104/185 (56.22%), Postives = 124/185 (67.03%), Query Frame = 0


Query: 1   MNRHNDPNPFDE--GEVNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNN 60
           MNR NDPNPFDE   EVNPF+    A GSK RIP++++ TLGFGQK DATVDIPLDTMN+
Sbjct: 1   MNRGNDPNPFDEEQPEVNPFAN---AAGSKSRIPKVVASTLGFGQKHDATVDIPLDTMND 60

Query: 61  LRKNERDIAAWEAELNRKEKEIKKREEAVSK----------------------------- 120
            +K E+++A WEA+LN++E+EIK+RE+AV+                              
Sbjct: 61  SKKKEKELANWEADLNKREREIKRREDAVASAGVPVDDRNWPPFFPIIHHDIANEIPVHA 120

Query: 121 ------------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPL 143
                       GI LCLVFNV+AITVCWIRGGG  IFLLAVIYA++G PLSYVLWYRPL
Sbjct: 121 QKLQYLAFASWLGIVLCLVFNVVAITVCWIRGGGAKIFLLAVIYALMGCPLSYVLWYRPL 180

[Close]

BLAST of Carg23580-RA vs. TrEMBL
Match: tr|A0A1U7VVR0|A0A1U7VVR0_NICSY (Secretory carrier-associated membrane protein OS=Nicotiana sylvestris OX=4096 GN=LOC104217457 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.3e-43
Identity = 101/185 (54.59%), Postives = 121/185 (65.41%), Query Frame = 0


Query: 1   MNRHNDPNPFDEGE--VNPFSKDNAAPGSKFRIPQMISDTLGFGQKQDATVDIPLDTMNN 60
           M++ NDPNPFDE E  +NPFS   +AP +K R PQMI+ TLGFGQK DATVDIPLD+MN+
Sbjct: 1   MSKGNDPNPFDEEEPQINPFSNGGSAPATKSRFPQMIASTLGFGQKNDATVDIPLDSMND 60

Query: 61  LRKNERDIAAWEAELNRKEKEIKKREEAVSK----------------------------- 120
            +K ++++AAW  +L RKE+EIK+RE+AV+                              
Sbjct: 61  PKKRQKELAAWAEDLERKEREIKRREDAVASAGVPTNDKNWPPFFPIIHHDIANEIPVHA 120

Query: 121 ------------GIGLCLVFNVIAITVCWIRGGGVTIFLLAVIYAILGIPLSYVLWYRPL 143
                       GI LCLVFNVIA+TVCWIR GGV IFLLAVIYA+LG PLSYVLWYRPL
Sbjct: 121 QRMQYLAFASWLGIVLCLVFNVIAVTVCWIRDGGVKIFLLAVIYALLGCPLSYVLWYRPL 180

The following BLAST results are available for this feature:

BLAST of Carg23580-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923600.1	1.6e-66	77.60	secretory carrier-associated membrane protein 4-like [Cucurbita moschata]	[more]
XP_022965309.1	3.6e-66	77.05	secretory carrier-associated membrane protein 4-like [Cucurbita maxima]	[more]
XP_023552124.1	8.0e-66	76.50	secretory carrier-associated membrane protein 4-like [Cucurbita pepo subsp. pepo...	[more]
XP_017234947.1	5.8e-48	66.90	PREDICTED: secretory carrier-associated membrane protein 4 isoform X2 [Daucus ca...	[more]
XP_011074780.1	2.9e-47	57.84	secretory carrier-associated membrane protein 4 [Sesamum indicum]	[more]

BLAST of Carg23580-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G32050.1	1.1e-37	50.27	SCAMP family protein	[more]
AT1G61250.1	8.7e-14	66.04	secretory carrier 3	[more]
AT1G03550.1	3.3e-13	34.78	Secretory carrier membrane protein (SCAMP) family protein	[more]
AT2G20840.1	3.3e-13	30.11	Secretory carrier membrane protein (SCAMP) family protein	[more]
AT1G11180.2	1.3e-12	32.18	Secretory carrier membrane protein (SCAMP) family protein	[more]

BLAST of Carg23580-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9C6X2\|SCAM4_ARATH	2.0e-36	50.27	Secretory carrier-associated membrane protein 4 OS=Arabidopsis thaliana OX=3702 ...	[more]
sp\|Q0JAI9\|SCAM6_ORYSJ	2.3e-19	36.02	Secretory carrier-associated membrane protein 6 OS=Oryza sativa subsp. japonica ...	[more]
sp\|Q6Z8F5\|SCAM5_ORYSJ	8.9e-16	32.47	Secretory carrier-associated membrane protein 5 OS=Oryza sativa subsp. japonica ...	[more]
sp\|Q9ZTX0\|SCAM_PEA	1.7e-14	33.52	Secretory carrier-associated membrane protein OS=Pisum sativum OX=3888 GN=PSAM2 ...	[more]
sp\|Q9SXA5\|SCAM5_ARATH	1.2e-12	34.39	Secretory carrier-associated membrane protein 5 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg23580-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2R6PHR2\|A0A2R6PHR2_ACTCH	2.5e-47	57.92	Secretory carrier-associated membrane protein OS=Actinidia chinensis var. chinen...	[more]
tr\|A0A1S3BK39\|A0A1S3BK39_CUCME	1.6e-46	60.11	Secretory carrier-associated membrane protein OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
tr\|A0A0A0L3U1\|A0A0A0L3U1_CUCSA	1.5e-44	58.47	Secretory carrier-associated membrane protein OS=Cucumis sativus OX=3659 GN=Csa_...	[more]
tr\|A0A2G9FZP0\|A0A2G9FZP0_9LAMI	7.5e-44	56.22	Secretory carrier-associated membrane protein OS=Handroanthus impetiginosus OX=4...	[more]
tr\|A0A1U7VVR0\|A0A1U7VVR0_NICSY	6.3e-43	54.59	Secretory carrier-associated membrane protein OS=Nicotiana sylvestris OX=4096 GN...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0015031	protein transport

Vocabulary: **INTERPRO**
Term	Definition
IPR007273	SCAMP

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23580	Carg23580	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23580-RA	Carg23580-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23580-RA.CDS.6	Carg23580-RA.CDS.6	CDS
Carg23580-RA.CDS.5	Carg23580-RA.CDS.5	CDS
Carg23580-RA.CDS.4	Carg23580-RA.CDS.4	CDS
Carg23580-RA.CDS.3	Carg23580-RA.CDS.3	CDS
Carg23580-RA.CDS.2	Carg23580-RA.CDS.2	CDS
Carg23580-RA.CDS.1	Carg23580-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23580-RA.exon.6	Carg23580-RA.exon.6	exon
Carg23580-RA.exon.5	Carg23580-RA.exon.5	exon
Carg23580-RA.exon.4	Carg23580-RA.exon.4	exon
Carg23580-RA.exon.3	Carg23580-RA.exon.3	exon
Carg23580-RA.exon.2	Carg23580-RA.exon.2	exon
Carg23580-RA.exon.1	Carg23580-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 59..86
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR10687:SF15	SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 4	coord: 1..89 coord: 90..142
IPR007273	SCAMP	PFAM	PF04144	SCAMP	coord: 91..142 e-value: 7.2E-14 score: 52.4
IPR007273	SCAMP	PANTHER	PTHR10687	SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP	coord: 1..89 coord: 90..142