Carg19957-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg19957-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	protein NRT1/ PTR FAMILY 5.6
Location	Cucurbita_argyrosperma_scaffold_167 : 65670 .. 67928 (-)
Sequence length	539

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAGTAGAGTCCAAGATACTGGGCGGTTGTTGTCCAGATCCAACTTATTGGGAGAACTGACAACCAAATGTGTGAGATGTCGAAAAGCTTGGAGTGTGAAGCTAATGGCGGCGATGACAAATGGGTTGCTGATTCTTCTCTTGATTACAAAGGGAGGCTGCCTCTCCGAGATTCCACCGGCGCTTGGAAAGCCTCTCTGTTCATCATCGGTATAACGCACTACTTCTTCCTCTGTTCTTCACCGGTGGATCAATTTTTGATTAATTACTAACAAAATTGGAATTTGGGATTGCAGCCATCGAGTTCGGTGAGAGGCTGAGCTATTTTGGGATCGCTTCGAGTTTGATTATATACCTAACCAAGGTGCTTCATCAAGAGCTGAAGACGGCGGCGAGAAGCGTCAATTACTGGGCCGGTGTCACCACTTTGATGCCTCTGCTCGGCGGCTTTCTCGCCGACGCTTACTTCGGCCGCTACGCCACCGTTCTCCTCTCCTCCGTTATCTACGTTTTGGTCGGTTTCATTTTCAATACCCCATTTGATTATTATTATTATTAATACCCAATTTTAAGATGTGAGTGATTGTTGAAACAGGGGTTGACTCTGTTAACCATGTCGGCGTTCGTTCCAAGTTTCAAACCCTGTGGAGGCGATGATCAGGTGTGCCCGGAGGCACGGAAGAGCCATGAGATCATCTTCTTCCTCGCAATTTACATGATCTCCATAGGAACTGGTGGGCACAAGCCATCTCTCGAGAGCTTCGGAGCCGACCAATTCGATGATGATCATTCGGAAGAACGGAAGAAAAAAATGTCTTATTTCAATTGGTGGAATTTCGGGCTCTGTTCTGGCCTCTTGTTTGGCGTTACCATCATTGTTTATATCCAGGATCACTTCGGGTGGGGAACTGCAGATGTCATTCTAACGGCGGTGCTGGTGGTTTCTGTCGTGATTTTTGTCGCCGGCCGCCCGTTTTATCGGTACCGAAAGCCATCTGGAAGCCCCTTGACGCCGTTGTTGCAGGTTGTTGTGGCGGCCATTCGGAAGAGAAAGCTTCCACTCCCTTCCAATCCTTCTTTATTGCATGAATTTCCCAGGACAGCTAATAACGCCGCCCATGGGAGGTTCTTGTGCCACACCCAAAACCTCAAGTAAGCAATCAAATCAAAATATTATTTTTATTTAGTGTCAAAGAGTGTTAGACGAACACGACTCTCCACAATGGTATGATATTGTCCACGTTGAGTATAAATTCTCATGGTTTTGCTTTGGGCTTCCCAAAAAGCTTCATAACAATGGAGTTAGTATTCCTCACTTATAAACTCATGATCATTCCCTAAATTAGCGGACCTGGAACTTCCATCGTTCAACAAAGATAACCCACCACACTAATTTAGGAAACCGTCATGAAATTAAAAAAATATTCTTTTCTCGATTGTGGAGAGAGTCATGATTCCTACACGATATAAGAGCTATGCCCTTAACTTAACTGTGTTAATAGAATTCTCTAATGTTGAATAAATAAGAGTCTCGAAGGTATAGTCAAAAGTGACTCATAAAAAAGAGTTTGGAAGCTGTAGTCAAAAGTGACTCAAGTGTCGAACAAATTGTGTACTTTGTTTGAAAAGTCTAGAGAAGGAGTCAAGCCTCGATTAATGGGAAGCTATTCAAGGGTTTGATAGGTCTTAGGGGAGGCTCTATAATTGTACTATGTTCGAGGGAGGGCTCCATAGATCTCAGAGGAGGATGAATACATCTTCATTGGTATGAGACATTTTGAGGAAGCCGAAAAAGATAATATCATATCATTGTGGATAAGTCTGATTTCTGACCCCGAGTAGAAATTAAGTTAAGAAAATTAAACTCCGAATTTGAATGTCCTAAATATTGTGCAATAACAAAATTGTTGATTGGGTAATAGATTTCTAGACAAAGCGGCGGTTTATGAAGACAAGGAGGGATCGGCGGAGAAGCAAAGTCCATGGCGGCTAGCGACGGTGACGAAGGTGGAAGAAATGAAGCTAATATTGAACATGATACCCATTTGGTTGGCGACTCTGCCGTTCGGAGTCACCATAGCTCAAACCTCGACGTTCTTCATAAAACAAGCCGCCAACTTGAACCGGAAGATGGGCGACGGCTTGATCCTCCCGCCGACCACCATCTTCTGCCTGACTGCCATAGGAATGATCGTGTCCCTCACCATTTATGATAAGCTGTTGGTGCCCATGCTCCGACGAGCCACCGGCAACGAGAGA

mRNA sequence

GCAAGTAGAGTCCAAGATACTGGGCGGTTGTTGTCCAGATCCAACTTATTGGGAGAACTGACAACCAAATGTGTGAGATGTCGAAAAGCTTGGAGTGTGAAGCTAATGGCGGCGATGACAAATGGGTTGCTGATTCTTCTCTTGATTACAAAGGGAGGCTGCCTCTCCGAGATTCCACCGGCGCTTGGAAAGCCTCTCTGTTCATCATCGACAAAGCGGCGGTTTATGAAGACAAGGAGGGATCGGCGGAGAAGCAAAGTCCATGGCGGCTAGCGACGGTGACGAAGGTGGAAGAAATGAAGCTAATATTGAACATGATACCCATTTGGTTGGCGACTCTGCCGTTCGGAGTCACCATAGCTCAAACCTCGACGTTCTTCATAAAACAAGCCGCCAACTTGAACCGGAAGATGGGCGACGGCTTGATCCTCCCGCCGACCACCATCTTCTGCCTGACTGCCATAGGAATGATCGTGTCCCTCACCATTTATGATAAGCTGTTGGTGCCCATGCTCCGACGAGCCACCGGCAACGAGAGA

Coding sequence (CDS)

ATGTGTGAGATGTCGAAAAGCTTGGAGTGTGAAGCTAATGGCGGCGATGACAAATGGGTTGCTGATTCTTCTCTTGATTACAAAGGGAGGCTGCCTCTCCGAGATTCCACCGGCGCTTGGAAAGCCTCTCTGTTCATCATCGACAAAGCGGCGGTTTATGAAGACAAGGAGGGATCGGCGGAGAAGCAAAGTCCATGGCGGCTAGCGACGGTGACGAAGGTGGAAGAAATGAAGCTAATATTGAACATGATACCCATTTGGTTGGCGACTCTGCCGTTCGGAGTCACCATAGCTCAAACCTCGACGTTCTTCATAAAACAAGCCGCCAACTTGAACCGGAAGATGGGCGACGGCTTGATCCTCCCGCCGACCACCATCTTCTGCCTGACTGCCATAGGAATGATCGTGTCCCTCACCATTTATGATAAGCTGTTGGTGCCCATGCTCCGACGAGCCACCGGCAACGAGAGA

Protein sequence

MCEMSKSLECEANGGDDKWVADSSLDYKGRLPLRDSTGAWKASLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTFFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER

Homology

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BLAST of Carg19957-RA vs. NCBI nr
Match: XP_022950472.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita moschata])

HSP 1 Score: 217.2 bits (552), Expect = 3.9e-53
Identity = 110/115 (95.65%), Postives = 112/115 (97.39%), Query Frame = 0


Query: 43  SLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 102
           +L  +DKAAVYEDK GSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST
Sbjct: 304 NLKFLDKAAVYEDKGGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 363

Query: 103 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER
Sbjct: 364 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 418

HSP 2 Score: 99.8 bits (247), Expect = 9.0e-18
Identity = 46/47 (97.87%), Postives = 46/47 (97.87%), Query Frame = 0


Query: 1  MCEMSKSLECEANGGDDKWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          MCEMSKSLECEANGGDDKWVADSSLDYKGRLPLR STGAWKASLFII
Sbjct: 1  MCEMSKSLECEANGGDDKWVADSSLDYKGRLPLRASTGAWKASLFII 47

[Close]

BLAST of Carg19957-RA vs. NCBI nr
Match: XP_023543405.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 217.2 bits (552), Expect = 3.9e-53
Identity = 110/115 (95.65%), Postives = 112/115 (97.39%), Query Frame = 0


Query: 43  SLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 102
           +L  +DKAAVYEDK GSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST
Sbjct: 304 NLKFLDKAAVYEDKGGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 363

Query: 103 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER
Sbjct: 364 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 418

HSP 2 Score: 97.1 bits (240), Expect = 5.8e-17
Identity = 45/47 (95.74%), Postives = 45/47 (95.74%), Query Frame = 0


Query: 1  MCEMSKSLECEANGGDDKWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          MCEMSKSLECEANGGD KWVADSSLDYKGRLPLR STGAWKASLFII
Sbjct: 1  MCEMSKSLECEANGGDHKWVADSSLDYKGRLPLRASTGAWKASLFII 47

[Close]

BLAST of Carg19957-RA vs. NCBI nr
Match: XP_022978121.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita maxima])

HSP 1 Score: 211.1 bits (536), Expect = 2.8e-51
Identity = 107/115 (93.04%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 43  SLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 102
           +L  +DKAAVYEDK GSAE QSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST
Sbjct: 304 NLKFLDKAAVYEDKGGSAEMQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 363

Query: 103 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQAANLNRKMGD LILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRR TGNER
Sbjct: 364 FFIKQAANLNRKMGDSLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRTTGNER 418

HSP 2 Score: 97.1 bits (240), Expect = 5.8e-17
Identity = 45/47 (95.74%), Postives = 45/47 (95.74%), Query Frame = 0


Query: 1  MCEMSKSLECEANGGDDKWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          MCEMSKSLECEANGGD KWVADSSLDYKGRLPLR STGAWKASLFII
Sbjct: 1  MCEMSKSLECEANGGDHKWVADSSLDYKGRLPLRASTGAWKASLFII 47

[Close]

BLAST of Carg19957-RA vs. NCBI nr
Match: XP_023517590.1 (protein NRT1/ PTR FAMILY 5.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 194.1 bits (492), Expect = 3.5e-46
Identity = 94/114 (82.46%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTF 103
           L  +DKAA+YE+    AEK+SPWRLATVTKVEEMKLILNMIPIWLATLPFGV + QTSTF
Sbjct: 316 LKFLDKAAIYEESGAPAEKESPWRLATVTKVEEMKLILNMIPIWLATLPFGVAVGQTSTF 375

Query: 104 FIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FIKQAANLNRK+ DGL LPPTTIFCL A+GMI+SLTIYDKLLVP+LRRATGNER
Sbjct: 376 FIKQAANLNRKISDGLTLPPTTIFCLAAVGMIISLTIYDKLLVPLLRRATGNER 429

HSP 2 Score: 59.7 bits (143), Expect = 1.0e-05
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 19 WVADSSLDYKGRLPLRDSTGAWKASLFII 48
          WVADSS+DYKGR+PLR STGAWKASLFII
Sbjct: 31 WVADSSVDYKGRVPLRASTGAWKASLFII 59

[Close]

BLAST of Carg19957-RA vs. NCBI nr
Match: XP_022151485.1 (protein NRT1/ PTR FAMILY 5.6-like [Momordica charantia])

HSP 1 Score: 193.7 bits (491), Expect = 4.6e-46
Identity = 96/115 (83.48%), Postives = 104/115 (90.43%), Query Frame = 0


Query: 43  SLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 102
           +L  +DKAAV E   G AEKQ+PWRLATVTKVEEMKLILNMIPIWLATLPFG+TIAQTST
Sbjct: 309 NLKFLDKAAVCEGTGGPAEKQNPWRLATVTKVEEMKLILNMIPIWLATLPFGMTIAQTST 368

Query: 103 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQAANLNRK+GDG +LPPTTIFCL A+ M+VSLTIYDKLLVPMLRR TGNER
Sbjct: 369 FFIKQAANLNRKIGDGFLLPPTTIFCLAAVAMLVSLTIYDKLLVPMLRRTTGNER 423

HSP 2 Score: 64.7 bits (156), Expect = 3.2e-07
Identity = 30/40 (75.00%), Postives = 34/40 (85.00%), Query Frame = 0


Query: 11 EANGG---DDKWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          E NGG   D+KWV DSS+DY+GR+PLR STGAWKASLFII
Sbjct: 15 EINGGGETDEKWVRDSSVDYRGRIPLRASTGAWKASLFII 54

[Close]

BLAST of Carg19957-RA vs. TAIR10
Match: AT3G53960.1 (Major facilitator superfamily protein)

HSP 1 Score: 140.2 bits (352), Expect = 1.1e-33
Identity = 72/118 (61.02%), Postives = 93/118 (78.81%), Query Frame = 0


Query: 43  SLFIIDKAAVYEDKEGS--AEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQT 102
           +L  +DKAAV ED+  +  AEKQSPWRLATVTKVEE+KL++NMIPIW  TL FGV   Q+
Sbjct: 301 NLKFLDKAAVIEDRNENTKAEKQSPWRLATVTKVEEVKLLINMIPIWFFTLAFGVCATQS 360

Query: 103 STFFIKQAANLNRKM-GDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           ST FIKQA  ++R + G   I+PP ++F L A+ +I+++TIY+KLLVP+LRRATGNER
Sbjct: 361 STLFIKQAIIMDRHITGTSFIVPPASLFSLIALSIIITVTIYEKLLVPLLRRATGNER 418

[Close]

BLAST of Carg19957-RA vs. TAIR10
Match: AT2G37900.1 (Major facilitator superfamily protein)

HSP 1 Score: 134.8 bits (338), Expect = 4.6e-32
Identity = 68/115 (59.13%), Postives = 88/115 (76.52%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGSA-EKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 103
           L  +DKAA+ EDK   A EKQSPWRL T+TKVEE KLI+N+IPIW +TL FG+   Q ST
Sbjct: 303 LKFLDKAAIIEDKNPLALEKQSPWRLLTLTKVEETKLIINVIPIWFSTLAFGICATQAST 362

Query: 104 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQA  ++R +G G  +PP ++F LTA+ +I+SLT+Y+KLLVP+LR  T N+R
Sbjct: 363 FFIKQAITMDRHIG-GFTVPPASMFTLTALTLIISLTVYEKLLVPLLRSITRNQR 416

HSP 2 Score: 52.0 bits (123), Expect = 3.9e-07
Identity = 22/30 (73.33%), Postives = 26/30 (86.67%), Query Frame = 0


Query: 18 KWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          KWV DSSLD +GR+PLR  TGAW+A+LFII
Sbjct: 20 KWVLDSSLDSRGRVPLRARTGAWRAALFII 49

[Close]

BLAST of Carg19957-RA vs. TAIR10
Match: AT3G54450.1 (Major facilitator superfamily protein)

HSP 1 Score: 93.2 bits (230), Expect = 1.5e-19
Identity = 39/109 (35.78%), Postives = 75/109 (68.81%), Query Frame = 0


Query: 47  IDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTFFIK 106
           +DKA + ++ + + + ++PWRL TV +VEE+KLIL +IPIW++ + F  T+ Q +TFF+K
Sbjct: 275 LDKATIIDEIDHN-KNRNPWRLCTVNQVEEVKLILRLIPIWISLIMFCATLTQLNTFFLK 334

Query: 107 QAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGN 156
           Q + ++R +G+   +PP     +  + +++ + +YD++ VPM+R+ T +
Sbjct: 335 QGSMMDRTIGNHFTIPPAAFQSIVGVTILILIPLYDRVFVPMVRKITNH 382

[Close]

BLAST of Carg19957-RA vs. TAIR10
Match: AT5G46040.1 (Major facilitator superfamily protein)

HSP 1 Score: 85.5 bits (210), Expect = 3.2e-17
Identity = 44/116 (37.93%), Postives = 70/116 (60.34%), Query Frame = 0


Query: 42  ASLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTS 101
           +SL  +++A++   K GS  K   WRL T+T+VEE K +L M+P+   T    + +AQ  
Sbjct: 297 SSLRFLNRASL---KTGSTHK---WRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIM 356

Query: 102 TFFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           T FIKQ   L+R++ +   +PP ++   T   M+VS+ IYD++ V  +R+ TGN R
Sbjct: 357 TLFIKQGTTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPR 406

[Close]

BLAST of Carg19957-RA vs. TAIR10
Match: AT2G02040.1 (peptide transporter 2)

HSP 1 Score: 85.1 bits (209), Expect = 4.2e-17
Identity = 42/112 (37.50%), Postives = 69/112 (61.61%), Query Frame = 0


Query: 47  IDKAAVYEDKEG-SAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTFFI 106
           +DKAAV  ++E  S +  + WRL TVT+VEE+K+++ M PIW + + F    AQ ST F+
Sbjct: 313 LDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFV 372

Query: 107 KQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           +Q   +N K+G    LPP  +       +I+ + +YD+ +VP+ R+ TG ++
Sbjct: 373 QQGRAMNCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDK 423

[Close]

BLAST of Carg19957-RA vs. Swiss-Prot
Match: sp|Q9M331|PTR45_ARATH (Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 2.0e-32
Identity = 72/118 (61.02%), Postives = 93/118 (78.81%), Query Frame = 0


Query: 43  SLFIIDKAAVYEDKEGS--AEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQT 102
           +L  +DKAAV ED+  +  AEKQSPWRLATVTKVEE+KL++NMIPIW  TL FGV   Q+
Sbjct: 301 NLKFLDKAAVIEDRNENTKAEKQSPWRLATVTKVEEVKLLINMIPIWFFTLAFGVCATQS 360

Query: 103 STFFIKQAANLNRKM-GDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           ST FIKQA  ++R + G   I+PP ++F L A+ +I+++TIY+KLLVP+LRRATGNER
Sbjct: 361 STLFIKQAIIMDRHITGTSFIVPPASLFSLIALSIIITVTIYEKLLVPLLRRATGNER 418

[Close]

BLAST of Carg19957-RA vs. Swiss-Prot
Match: sp|P0CI03|PTR28_ARATH (Protein NRT1/ PTR FAMILY 5.6 OS=Arabidopsis thaliana OX=3702 GN=NPF5.6 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 8.3e-31
Identity = 68/115 (59.13%), Postives = 88/115 (76.52%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGSA-EKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 103
           L  +DKAA+ EDK   A EKQSPWRL T+TKVEE KLI+N+IPIW +TL FG+   Q ST
Sbjct: 303 LKFLDKAAIIEDKNPLALEKQSPWRLLTLTKVEETKLIINVIPIWFSTLAFGICATQAST 362

Query: 104 FFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQA  ++R +G G  +PP ++F LTA+ +I+SLT+Y+KLLVP+LR  T N+R
Sbjct: 363 FFIKQAITMDRHIG-GFTVPPASMFTLTALTLIISLTVYEKLLVPLLRSITRNQR 416

HSP 2 Score: 52.0 bits (123), Expect = 7.0e-06
Identity = 22/30 (73.33%), Postives = 26/30 (86.67%), Query Frame = 0


Query: 18 KWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          KWV DSSLD +GR+PLR  TGAW+A+LFII
Sbjct: 20 KWVLDSSLDSRGRVPLRARTGAWRAALFII 49

[Close]

BLAST of Carg19957-RA vs. Swiss-Prot
Match: sp|Q9M1I2|PTR46_ARATH (Protein NRT1/ PTR FAMILY 5.4 OS=Arabidopsis thaliana OX=3702 GN=NPF5.4 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.8e-18
Identity = 39/109 (35.78%), Postives = 75/109 (68.81%), Query Frame = 0


Query: 47  IDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTFFIK 106
           +DKA + ++ + + + ++PWRL TV +VEE+KLIL +IPIW++ + F  T+ Q +TFF+K
Sbjct: 275 LDKATIIDEIDHN-KNRNPWRLCTVNQVEEVKLILRLIPIWISLIMFCATLTQLNTFFLK 334

Query: 107 QAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGN 156
           Q + ++R +G+   +PP     +  + +++ + +YD++ VPM+R+ T +
Sbjct: 335 QGSMMDRTIGNHFTIPPAAFQSIVGVTILILIPLYDRVFVPMVRKITNH 382

[Close]

BLAST of Carg19957-RA vs. Swiss-Prot
Match: sp|Q9FNL8|PTR4_ARATH (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.7e-16
Identity = 44/116 (37.93%), Postives = 70/116 (60.34%), Query Frame = 0


Query: 42  ASLFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTS 101
           +SL  +++A++   K GS  K   WRL T+T+VEE K +L M+P+   T    + +AQ  
Sbjct: 297 SSLRFLNRASL---KTGSTHK---WRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIM 356

Query: 102 TFFIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           T FIKQ   L+R++ +   +PP ++   T   M+VS+ IYD++ V  +R+ TGN R
Sbjct: 357 TLFIKQGTTLDRRLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPR 406

[Close]

BLAST of Carg19957-RA vs. Swiss-Prot
Match: sp|P46032|PTR2_ARATH (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.5e-16
Identity = 42/112 (37.50%), Postives = 69/112 (61.61%), Query Frame = 0


Query: 47  IDKAAVYEDKEG-SAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTFFI 106
           +DKAAV  ++E  S +  + WRL TVT+VEE+K+++ M PIW + + F    AQ ST F+
Sbjct: 313 LDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFV 372

Query: 107 KQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           +Q   +N K+G    LPP  +       +I+ + +YD+ +VP+ R+ TG ++
Sbjct: 373 QQGRAMNCKIG-SFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDK 423

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BLAST of Carg19957-RA vs. TrEMBL
Match: tr|A0A0A0KXC6|A0A0A0KXC6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303040 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.0e-46
Identity = 99/116 (85.34%), Postives = 107/116 (92.24%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGS-AEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 103
           L  +DKAAVYE+  G  AEKQSPWRLATVTKVEEMKLILNMIPIWL+TLPFGVTIAQTST
Sbjct: 312 LKFLDKAAVYEENNGGPAEKQSPWRLATVTKVEEMKLILNMIPIWLSTLPFGVTIAQTST 371

Query: 104 FFIKQAANLNRKMGD-GLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQA+N+NRK+GD GLILPPTTIFCL AIGMIVS+TIYDK+LVPMLRR TGNER
Sbjct: 372 FFIKQASNMNRKIGDGGLILPPTTIFCLAAIGMIVSITIYDKVLVPMLRRTTGNER 427

HSP 2 Score: 59.7 bits (143), Expect = 6.8e-06
Identity = 26/29 (89.66%), Postives = 28/29 (96.55%), Query Frame = 0


Query: 19 WVADSSLDYKGRLPLRDSTGAWKASLFII 48
          WVADSS+DYKGR+PLR STGAWKASLFII
Sbjct: 27 WVADSSVDYKGRVPLRASTGAWKASLFII 55

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BLAST of Carg19957-RA vs. TrEMBL
Match: tr|A0A1S3BJN5|A0A1S3BJN5_CUCME (protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo OX=3656 GN=LOC103490633 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.2e-46
Identity = 98/116 (84.48%), Postives = 107/116 (92.24%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGS-AEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTST 103
           L  +DKAA+YE+  G  AEKQSPWRLATVTKVEEMKLILNMIPIWL+TLPFGVTIAQTST
Sbjct: 310 LKFLDKAAIYEENNGGPAEKQSPWRLATVTKVEEMKLILNMIPIWLSTLPFGVTIAQTST 369

Query: 104 FFIKQAANLNRKMGD-GLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FFIKQA+N+NRK+GD GLILPPTTIFCL AIGMIVS+TIYDK+LVPMLRR TGNER
Sbjct: 370 FFIKQASNMNRKIGDGGLILPPTTIFCLAAIGMIVSITIYDKVLVPMLRRTTGNER 425

HSP 2 Score: 61.6 bits (148), Expect = 1.8e-06
Identity = 38/78 (48.72%), Postives = 47/78 (60.26%), Query Frame = 0


Query: 17 DKWVADSSLDYKGRLPLRDSTGAWKASLFIIDKAAVYEDKEGSAEKQSPWRLAT-----V 76
          +KWVADSS+DYKGR+PLR STGAWKASLFII             E+ S + +AT     +
Sbjct: 23 EKWVADSSVDYKGRVPLRASTGAWKASLFIIAME--------FGERLSYFGIATSLIIYL 82

Query: 77 TKV--EEMKLILNMIPIW 88
          TKV  EE+K     +  W
Sbjct: 83 TKVLHEELKTAARSVNYW 92

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BLAST of Carg19957-RA vs. TrEMBL
Match: tr|A0A2C9VEI3|A0A2C9VEI3_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G078700 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.7e-39
Identity = 80/114 (70.18%), Postives = 98/114 (85.96%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTF 103
           L  +DKAA+ ED E +AEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFG+ +AQ +TF
Sbjct: 299 LKFLDKAAILEDTENAAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGICVAQAATF 358

Query: 104 FIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FIKQ   +NR++ +G ++PP +I+ LTAIGMI S+TIY+K+LVP+LRR TGNER
Sbjct: 359 FIKQCTTMNREISNGFLIPPASIYALTAIGMIFSVTIYEKVLVPILRRVTGNER 412

HSP 2 Score: 57.8 bits (138), Expect = 2.6e-05
Identity = 24/32 (75.00%), Postives = 28/32 (87.50%), Query Frame = 0


Query: 16 DDKWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          +DKWV DSS+D+KGR+PLR STG WK SLFII
Sbjct: 14 EDKWVYDSSVDHKGRVPLRSSTGVWKGSLFII 45

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BLAST of Carg19957-RA vs. TrEMBL
Match: tr|A0A2I4DZA8|A0A2I4DZA8_9ROSI (protein NRT1/ PTR FAMILY 5.6-like OS=Juglans regia OX=51240 GN=LOC108984860 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-38
Identity = 80/114 (70.18%), Postives = 98/114 (85.96%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTF 103
           L  +DKAAV ED +  AEKQSPW+LATVTKVEE+KL+LNMIPIWLATLPFG+ IAQ +TF
Sbjct: 304 LKFLDKAAVLEDNDNPAEKQSPWKLATVTKVEELKLVLNMIPIWLATLPFGICIAQGTTF 363

Query: 104 FIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FIKQ A LNRK+G+G  LPP +I+ + AIGMI+S+TIY+K+LVP +R+ATGNER
Sbjct: 364 FIKQGAILNRKIGNGFELPPASIYAVAAIGMIISVTIYEKILVPTIRKATGNER 417

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BLAST of Carg19957-RA vs. TrEMBL
Match: tr|A0A1Q3BBM5|A0A1Q3BBM5_CEPFO (PTR2 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_08962 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-38
Identity = 79/114 (69.30%), Postives = 97/114 (85.09%), Query Frame = 0


Query: 44  LFIIDKAAVYEDKEGSAEKQSPWRLATVTKVEEMKLILNMIPIWLATLPFGVTIAQTSTF 103
           L  +DKAA+ ED+  S EKQSPWRLATVTKVEEMKL+LN+IPIWLATLPFGV +AQ STF
Sbjct: 299 LKFLDKAAIIEDEGNSDEKQSPWRLATVTKVEEMKLLLNIIPIWLATLPFGVCVAQASTF 358

Query: 104 FIKQAANLNRKMGDGLILPPTTIFCLTAIGMIVSLTIYDKLLVPMLRRATGNER 158
           FIKQ   LNRK+ D  ++PP +++ L AIGMI+S+T+Y+K+LVP+LRRATGNER
Sbjct: 359 FIKQGTTLNRKIVDDFLIPPASVYSLAAIGMIISVTVYEKILVPLLRRATGNER 412

HSP 2 Score: 57.8 bits (138), Expect = 2.6e-05
Identity = 28/38 (73.68%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 11 EANGGDD-KWVADSSLDYKGRLPLRDSTGAWKASLFII 48
          EA   DD KWV DSS+DYKGR+P R STG WKASLFII
Sbjct: 8  EARDADDKKWVYDSSVDYKGRVPHRASTGVWKASLFII 45

The following BLAST results are available for this feature:

BLAST of Carg19957-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950472.1	3.9e-53	95.65	protein NRT1/ PTR FAMILY 5.6-like [Cucurbita moschata]	[more]
XP_023543405.1	3.9e-53	95.65	protein NRT1/ PTR FAMILY 5.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978121.1	2.8e-51	93.04	protein NRT1/ PTR FAMILY 5.6-like [Cucurbita maxima]	[more]
XP_023517590.1	3.5e-46	82.46	protein NRT1/ PTR FAMILY 5.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022151485.1	4.6e-46	83.48	protein NRT1/ PTR FAMILY 5.6-like [Momordica charantia]	[more]

BLAST of Carg19957-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT3G53960.1	1.1e-33	61.02	Major facilitator superfamily protein	[more]
AT2G37900.1	4.6e-32	59.13	Major facilitator superfamily protein	[more]
AT3G54450.1	1.5e-19	35.78	Major facilitator superfamily protein	[more]
AT5G46040.1	3.2e-17	37.93	Major facilitator superfamily protein	[more]
AT2G02040.1	4.2e-17	37.50	peptide transporter 2	[more]

BLAST of Carg19957-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9M331\|PTR45_ARATH	2.0e-32	61.02	Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2	[more]
sp\|P0CI03\|PTR28_ARATH	8.3e-31	59.13	Protein NRT1/ PTR FAMILY 5.6 OS=Arabidopsis thaliana OX=3702 GN=NPF5.6 PE=2 SV=1	[more]
sp\|Q9M1I2\|PTR46_ARATH	2.8e-18	35.78	Protein NRT1/ PTR FAMILY 5.4 OS=Arabidopsis thaliana OX=3702 GN=NPF5.4 PE=2 SV=1	[more]
sp\|Q9FNL8\|PTR4_ARATH	5.7e-16	37.93	Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1	[more]
sp\|P46032\|PTR2_ARATH	7.5e-16	37.50	Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1	[more]

BLAST of Carg19957-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KXC6\|A0A0A0KXC6_CUCSA	4.0e-46	85.34	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G303040 PE=4 SV=1	[more]
tr\|A0A1S3BJN5\|A0A1S3BJN5_CUCME	5.2e-46	84.48	protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo OX=3656 GN=LOC103490633 PE=4 SV=1	[more]
tr\|A0A2C9VEI3\|A0A2C9VEI3_MANES	2.7e-39	70.18	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G078700 PE=4 SV=...	[more]
tr\|A0A2I4DZA8\|A0A2I4DZA8_9ROSI	1.8e-38	70.18	protein NRT1/ PTR FAMILY 5.6-like OS=Juglans regia OX=51240 GN=LOC108984860 PE=4...	[more]
tr\|A0A1Q3BBM5\|A0A1Q3BBM5_CEPFO	1.8e-38	69.30	PTR2 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_089...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0022857	transmembrane transporter activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0055085	transmembrane transport

Vocabulary: **INTERPRO**
Term	Definition
IPR000109	POT_fam

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg19957	Carg19957	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg19957-RA.CDS.2	Carg19957-RA.CDS.2	CDS
Carg19957-RA.CDS.1	Carg19957-RA.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg19957-RA.five_prime_UTR.1	Carg19957-RA.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg19957-RA.exon.2	Carg19957-RA.exon.2	exon
Carg19957-RA.exon.1	Carg19957-RA.exon.1	exon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg19957-RA	Carg19957-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:1.20.1250.20		coord: 47..157 e-value: 7.8E-25 score: 88.9
None	No IPR available	PANTHER	PTHR11654:SF293	PROTEIN NRT1/ PTR FAMILY 5.6-RELATED	coord: 47..157 coord: 15..47
IPR000109	Proton-dependent oligopeptide transporter family	PFAM	PF00854	PTR2	coord: 53..153 e-value: 1.9E-16 score: 59.8
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 47..157
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 15..47