Carg15084-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg15084-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Folylpolyglutamate synthase
Location	Cucurbita_argyrosperma_scaffold_078 : 454860 .. 455891 (-)
Sequence length	697

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAGGATTGTCGCAAGTCCAAGTGTTCCAACAGTTCTCGCTCAATCAGCTATTCTTCAAGCTTGCAAGTCGCATTTCCTACCATTTTTTTTCTCAAGAAAGAATGCCCATTTCACAATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGTTGGTTATTTATGATGATCAACTCTTTACTTTTCTTAGTTTTCACTAAATTTTGAAGTTTTGGACCGACCCATTTCCTCTGCAATTCACTAAATTTAGTGTTTTATTCATATTAATTCTCTCCTCACTTGTGATGGATATCAATACCAATCGATGGGAAATGACACTACCAATGTTTGAACATTGCATATGAAAAGTTTAAGCTGTTAGTGACTGATGGTAGGACAATTATAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGGTACAGCCTTGTTGCAACACTTTTCAAATTTTTCGTATACTCCTACTTGGAAGTGATTTGAGAAGGCATAACTTTGTGCTTGTGAATTTCTTCATGTGCAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

mRNA sequence

GAGGAGGATTGTCGCAAGTCCAAGTGTTCCAACAGTTCTCGCTCAATCAGCTATTCTTCAAGCTTGCAAGTCGCATTTCCTACCATTTTTTTTCTCAAGAAAGAATGCCCATTTCACAATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

Coding sequence (CDS)

ATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

Protein sequence

MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLYRAVCCCD

Homology

[Close]

BLAST of Carg15084-RA vs. NCBI nr
Match: XP_022922899.1 (dihydrofolate synthetase isoform X2 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. NCBI nr
Match: XP_022922901.1 (dihydrofolate synthetase isoform X4 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. NCBI nr
Match: XP_022922900.1 (dihydrofolate synthetase isoform X3 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. NCBI nr
Match: XP_022922898.1 (dihydrofolate synthetase isoform X1 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.8 bits (948), Expect = 5.7e-99
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQ MTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. TAIR10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein)

HSP 1 Score: 189.1 bits (479), Expect = 2.5e-48
Identity = 92/141 (65.25%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 44  SEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP GAGT+S+DGFDLGRMKRLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KELLDQSVKLENGRISHFEVL 185
           K +L+QS++ ENG +SHFE+L
Sbjct: 160 KPILEQSIQEENGSLSHFEIL 178

[Close]

BLAST of Carg15084-RA vs. TAIR10
Match: AT3G55630.3 (DHFS-FPGS homolog D)

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-09
Identity = 34/81 (41.98%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L    E+   K 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKF 115

Query: 156 LNFL---FKRNKELLDQSVKL 174
           +N+    F + KE     V +
Sbjct: 116 VNYFWCCFHKLKEKTSNEVPM 135

[Close]

BLAST of Carg15084-RA vs. TAIR10
Match: AT5G05980.1 (DHFS-FPGS homolog B)

HSP 1 Score: 58.5 bits (140), Expect = 5.1e-09
Identity = 45/140 (32.14%), Postives = 64/140 (45.71%), Query Frame = 0


Query: 5   KYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEK 64
           + HHH+  Q H   L +    +  S+  +I      G+  E     E +  L SL     
Sbjct: 30  RLHHHY--QHHQPHLPSPLSFQIHSLRKQIDMAAQGGDSYE-----EALAALSSLITKRS 89

Query: 65  LGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEG 124
               +  G   E  FD  ++  L E +     K   IH+AGTKGKGST  F  +I+R  G
Sbjct: 90  RADKSNKGDRFELVFDYLKLLDLEEDI----LKMNVIHVAGTKGKGSTCTFTESIIRNYG 149

Query: 125 YSVGCYTSPHIETIRERISL 145
           +  G +TSPH+  +RER  L
Sbjct: 150 FRTGLFTSPHLIDVRERFRL 158

[Close]

BLAST of Carg15084-RA vs. TAIR10
Match: AT3G10160.1 (DHFS-FPGS homolog C)

HSP 1 Score: 56.2 bits (134), Expect = 2.5e-08
Identity = 25/48 (52.08%), Postives = 32/48 (66.67%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISL 145
           + K IH+AGTKGKGST  F   ILR  G+  G +TSPH+  +RER  +
Sbjct: 130 ELKVIHVAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRI 177

[Close]

BLAST of Carg15084-RA vs. Swiss-Prot
Match: sp|F4JYE9|DHFS_ARATH (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.5e-47
Identity = 92/141 (65.25%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 44  SEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP GAGT+S+DGFDLGRMKRLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KELLDQSVKLENGRISHFEVL 185
           K +L+QS++ ENG +SHFE+L
Sbjct: 160 KPILEQSIQEENGSLSHFEIL 178

[Close]

BLAST of Carg15084-RA vs. Swiss-Prot
Match: sp|Q05865|FOLC_BACSU (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 7.5e-18
Identity = 46/104 (44.23%), Postives = 70/104 (67.31%), Query Frame = 0


Query: 81  LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRMK+LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
           RIS+  +G  +S +    L  + K  ++   + E G+ + FE++
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIM 125

[Close]

BLAST of Carg15084-RA vs. Swiss-Prot
Match: sp|Q12676|FOLD_YEAST (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FOL3 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 27/64 (42.19%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 81  LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           L R+ +L+E LGNPQ+  + +HIAGT GKGS   +LS++L+ + Y +G +T+PH+  + +
Sbjct: 7   LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66

Query: 141 RISL 145
            I++
Sbjct: 67  SITI 70

[Close]

BLAST of Carg15084-RA vs. Swiss-Prot
Match: sp|Q54CY5|FOLC_DICDI (Putative folylpolyglutamate synthase OS=Dictyostelium discoideum OX=44689 GN=folC PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.4e-10
Identity = 39/104 (37.50%), Postives = 58/104 (55.77%), Query Frame = 0


Query: 74  ESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSP 133
           E    F +  M+   + L     +   IH+AGTKGKGST A   +I+R +G+S G +TSP
Sbjct: 110 EESAKFLMEEMRNYCKTLSIDLERESIIHVAGTKGKGSTCAITESIIREQGFSTGLFTSP 169

Query: 134 HIETIRERISLGRSGEMVSGKALNFLFKRNKELL--DQSVKLEN 176
           H+ + RERI +  +GEM+S +  +  F    +LL  D   +L N
Sbjct: 170 HLISPRERIRI--NGEMISKEMFSQYFWNCWDLLIKDYQTQLPN 211

[Close]

BLAST of Carg15084-RA vs. Swiss-Prot
Match: sp|P15925|FPGS_LACCA (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.7e-09
Identity = 36/95 (37.89%), Postives = 49/95 (51.58%), Query Frame = 0


Query: 50  TEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGK 109
           TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT GK
Sbjct: 4   TETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGTNGK 63

Query: 110 GSTAAFLSNILRAEGYSVGCYTSPHIETIRERISL 145
           GS A  ++++L A G +VG YTSP I    ERI +
Sbjct: 64  GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMI 85

[Close]

BLAST of Carg15084-RA vs. TrEMBL
Match: tr|A0A1S4E568|A0A1S4E568_CUCME (dihydrofolate synthetase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.5e-87
Identity = 162/184 (88.04%), Postives = 172/184 (93.48%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALN LFKRNKE+ DQSV+LE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. TrEMBL
Match: tr|A0A1S3CNR7|A0A1S3CNR7_CUCME (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.5e-87
Identity = 162/184 (88.04%), Postives = 172/184 (93.48%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALN LFKRNKE+ DQSV+LE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. TrEMBL
Match: tr|A0A0A0L7A4|A0A0A0L7A4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-86
Identity = 158/184 (85.87%), Postives = 171/184 (92.93%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP G+GT+S+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084-RA vs. TrEMBL
Match: tr|A0A2I4H7G0|A0A2I4H7G0_9ROSI (dihydrofolate synthetase isoform X1 OS=Juglans regia OX=51240 GN=LOC109014167 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.5e-60
Identity = 115/152 (75.66%), Postives = 133/152 (87.50%), Query Frame = 0


Query: 33  RIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLG 92
           ++GSK+CF   SED ++ +FMEY+DSLKNYEKLGVP G+GT+SEDGFDLGRM+RLMERLG
Sbjct: 30  QVGSKRCFSTQSEDPELNDFMEYMDSLKNYEKLGVPRGSGTDSEDGFDLGRMRRLMERLG 89

Query: 93  NPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVS 152
           NPQSKF A+HIAGTKGKGSTAAFLSNILRAEGYSVGCY+SPHI+TIRERISLG     VS
Sbjct: 90  NPQSKFMAVHIAGTKGKGSTAAFLSNILRAEGYSVGCYSSPHIQTIRERISLGGFSAPVS 149

Query: 153 GKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
            K LN LF + K+ LDQ+++ ENGRISHFEVL
Sbjct: 150 AKTLNCLFLQFKKTLDQAMERENGRISHFEVL 181

[Close]

BLAST of Carg15084-RA vs. TrEMBL
Match: tr|A0A2I4H7E2|A0A2I4H7E2_9ROSI (dihydrofolate synthetase isoform X2 OS=Juglans regia OX=51240 GN=LOC109014167 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.5e-60
Identity = 115/152 (75.66%), Postives = 133/152 (87.50%), Query Frame = 0


Query: 33  RIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLG 92
           ++GSK+CF   SED ++ +FMEY+DSLKNYEKLGVP G+GT+SEDGFDLGRM+RLMERLG
Sbjct: 30  QVGSKRCFSTQSEDPELNDFMEYMDSLKNYEKLGVPRGSGTDSEDGFDLGRMRRLMERLG 89

Query: 93  NPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVS 152
           NPQSKF A+HIAGTKGKGSTAAFLSNILRAEGYSVGCY+SPHI+TIRERISLG     VS
Sbjct: 90  NPQSKFMAVHIAGTKGKGSTAAFLSNILRAEGYSVGCYSSPHIQTIRERISLGGFSAPVS 149

Query: 153 GKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
            K LN LF + K+ LDQ+++ ENGRISHFEVL
Sbjct: 150 AKTLNCLFLQFKKTLDQAMERENGRISHFEVL 181

The following BLAST results are available for this feature:

BLAST of Carg15084-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922899.1	2.6e-99	98.91	dihydrofolate synthetase isoform X2 [Cucurbita moschata]	[more]
XP_022922901.1	2.6e-99	98.91	dihydrofolate synthetase isoform X4 [Cucurbita moschata]	[more]
XP_022922900.1	2.6e-99	98.91	dihydrofolate synthetase isoform X3 [Cucurbita moschata]	[more]
XP_022922898.1	2.6e-99	98.91	dihydrofolate synthetase isoform X1 [Cucurbita moschata]	[more]
XP_023553540.1	5.7e-99	98.37	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg15084-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G41480.1	2.5e-48	65.25	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	1.8e-09	41.98	DHFS-FPGS homolog D	[more]
AT5G05980.1	5.1e-09	32.14	DHFS-FPGS homolog B	[more]
AT3G10160.1	2.5e-08	52.08	DHFS-FPGS homolog C	[more]

BLAST of Carg15084-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|F4JYE9\|DHFS_ARATH	4.5e-47	65.25	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
sp\|Q05865\|FOLC_BACSU	7.5e-18	44.23	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
sp\|Q12676\|FOLD_YEAST	3.1e-11	42.19	Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
sp\|Q54CY5\|FOLC_DICDI	4.4e-10	37.50	Putative folylpolyglutamate synthase OS=Dictyostelium discoideum OX=44689 GN=fol...	[more]
sp\|P15925\|FPGS_LACCA	1.7e-09	37.89	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]

BLAST of Carg15084-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4E568\|A0A1S4E568_CUCME	2.5e-87	88.04	dihydrofolate synthetase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4...	[more]
tr\|A0A1S3CNR7\|A0A1S3CNR7_CUCME	2.5e-87	88.04	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4...	[more]
tr\|A0A0A0L7A4\|A0A0A0L7A4_CUCSA	1.6e-86	85.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=4 SV=1	[more]
tr\|A0A2I4H7G0\|A0A2I4H7G0_9ROSI	3.5e-60	75.66	dihydrofolate synthetase isoform X1 OS=Juglans regia OX=51240 GN=LOC109014167 PE...	[more]
tr\|A0A2I4H7E2\|A0A2I4H7E2_9ROSI	3.5e-60	75.66	dihydrofolate synthetase isoform X2 OS=Juglans regia OX=51240 GN=LOC109014167 PE...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004326	tetrahydrofolylpolyglutamate synthase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0009396	folic acid-containing compound biosynthetic process
GO:0009058	biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR018109	Folylpolyglutamate_synth_CS
IPR001645	Folylpolyglutamate_synth
IPR036565	Mur-like_cat_sf

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
biological_process	GO:0046656	folic acid biosynthetic process
biological_process	GO:0006536	glutamate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg15084	Carg15084	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg15084-RA	Carg15084-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg15084-RA.CDS.3	Carg15084-RA.CDS.3	CDS
Carg15084-RA.CDS.2	Carg15084-RA.CDS.2	CDS
Carg15084-RA.CDS.1	Carg15084-RA.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg15084-RA.five_prime_UTR.1	Carg15084-RA.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg15084-RA.exon.3	Carg15084-RA.exon.3	exon
Carg15084-RA.exon.2	Carg15084-RA.exon.2	exon
Carg15084-RA.exon.1	Carg15084-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	G3DSA:3.40.1190.10		coord: 38..190 e-value: 4.8E-37 score: 129.5
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	SSF53623	MurD-like peptide ligases, catalytic domain	coord: 77..186
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 43..184
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 43..184
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124