Carg15084 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg15084
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Folylpolyglutamate synthase
Location	Cucurbita_argyrosperma_scaffold_078 : 454860 .. 455891 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAGGATTGTCGCAAGTCCAAGTGTTCCAACAGTTCTCGCTCAATCAGCTATTCTTCAAGCTTGCAAGTCGCATTTCCTACCATTTTTTTTCTCAAGAAAGAATGCCCATTTCACAATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGTTGGTTATTTATGATGATCAACTCTTTACTTTTCTTAGTTTTCACTAAATTTTGAAGTTTTGGACCGACCCATTTCCTCTGCAATTCACTAAATTTAGTGTTTTATTCATATTAATTCTCTCCTCACTTGTGATGGATATCAATACCAATCGATGGGAAATGACACTACCAATGTTTGAACATTGCATATGAAAAGTTTAAGCTGTTAGTGACTGATGGTAGGACAATTATAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGGTACAGCCTTGTTGCAACACTTTTCAAATTTTTCGTATACTCCTACTTGGAAGTGATTTGAGAAGGCATAACTTTGTGCTTGTGAATTTCTTCATGTGCAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

mRNA sequence

GAGGAGGATTGTCGCAAGTCCAAGTGTTCCAACAGTTCTCGCTCAATCAGCTATTCTTCAAGCTTGCAAGTCGCATTTCCTACCATTTTTTTTCTCAAGAAAGAATGCCCATTTCACAATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

Coding sequence (CDS)

ATGAATCTCTTCAAATATCATCATCACTTCCGCCCGCAGATTCACGGAAGGCTTCTCCTAAATTATTTCGTTGGTGAAGGGCCTTCTATCAGTAGCCGAATTGGGTCCAAACAATGCTTCGGCAATCACTCAGAAGATCAAGACATGACGGAATTCATGGAATACTTGGATTCCCTGAAGAACTACGAGAAGTTGGGTGTGCCCACAGGCGCAGGGACGGAGTCTGAGGATGGGTTCGATCTCGGAAGGATGAAAAGACTGATGGAGCGCCTGGGTAATCCACAATCCAAGTTTAAGGCAATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCGGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGTTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTTGGTAGATCTGGAGAGATGGTCTCTGGAAAGGCACTAAATTTTCTTTTCAAAAGGAACAAAGAGTTACTTGACCAATCGGTAAAACTTGAAAATGGACGTATAAGCCACTTCGAGGTATTATATCGTGCAGTCTGTTGTTGTGATTGA

Protein sequence

MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVLYRAVCCCD

Homology

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_022922899.1 (dihydrofolate synthetase isoform X2 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_022922901.1 (dihydrofolate synthetase isoform X4 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_022922900.1 (dihydrofolate synthetase isoform X3 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_022922898.1 (dihydrofolate synthetase isoform X1 [Cucurbita moschata])

HSP 1 Score: 370.9 bits (951), Expect = 2.6e-99
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCF NHSEDQDMTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.8 bits (948), Expect = 5.7e-99
Identity = 181/184 (98.37%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQ MTEFMEYLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPTGAGT+SEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. TAIR10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein)

HSP 1 Score: 189.1 bits (479), Expect = 2.5e-48
Identity = 92/141 (65.25%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 44  SEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP GAGT+S+DGFDLGRMKRLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KELLDQSVKLENGRISHFEVL 185
           K +L+QS++ ENG +SHFE+L
Sbjct: 160 KPILEQSIQEENGSLSHFEIL 178

[Close]

BLAST of Carg15084 vs. TAIR10
Match: AT3G55630.3 (DHFS-FPGS homolog D)

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-09
Identity = 34/81 (41.98%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L    E+   K 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL-NGIEISQEKF 115

Query: 156 LNFL---FKRNKELLDQSVKL 174
           +N+    F + KE     V +
Sbjct: 116 VNYFWCCFHKLKEKTSNEVPM 135

[Close]

BLAST of Carg15084 vs. TAIR10
Match: AT5G05980.1 (DHFS-FPGS homolog B)

HSP 1 Score: 58.5 bits (140), Expect = 5.1e-09
Identity = 45/140 (32.14%), Postives = 64/140 (45.71%), Query Frame = 0


Query: 5   KYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEK 64
           + HHH+  Q H   L +    +  S+  +I      G+  E     E +  L SL     
Sbjct: 30  RLHHHY--QHHQPHLPSPLSFQIHSLRKQIDMAAQGGDSYE-----EALAALSSLITKRS 89

Query: 65  LGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEG 124
               +  G   E  FD  ++  L E +     K   IH+AGTKGKGST  F  +I+R  G
Sbjct: 90  RADKSNKGDRFELVFDYLKLLDLEEDI----LKMNVIHVAGTKGKGSTCTFTESIIRNYG 149

Query: 125 YSVGCYTSPHIETIRERISL 145
           +  G +TSPH+  +RER  L
Sbjct: 150 FRTGLFTSPHLIDVRERFRL 158

[Close]

BLAST of Carg15084 vs. TAIR10
Match: AT3G10160.1 (DHFS-FPGS homolog C)

HSP 1 Score: 56.2 bits (134), Expect = 2.5e-08
Identity = 25/48 (52.08%), Postives = 32/48 (66.67%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISL 145
           + K IH+AGTKGKGST  F   ILR  G+  G +TSPH+  +RER  +
Sbjct: 130 ELKVIHVAGTKGKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRI 177

[Close]

BLAST of Carg15084 vs. Swiss-Prot
Match: sp|F4JYE9|DHFS_ARATH (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.5e-47
Identity = 92/141 (65.25%), Postives = 115/141 (81.56%), Query Frame = 0


Query: 44  SEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHI 103
           +ED ++ +F+ +L+SLKNYEK GVP GAGT+S+DGFDLGRMKRLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KELLDQSVKLENGRISHFEVL 185
           K +L+QS++ ENG +SHFE+L
Sbjct: 160 KPILEQSIQEENGSLSHFEIL 178

[Close]

BLAST of Carg15084 vs. Swiss-Prot
Match: sp|Q05865|FOLC_BACSU (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 7.5e-18
Identity = 46/104 (44.23%), Postives = 70/104 (67.31%), Query Frame = 0


Query: 81  LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRMK+LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
           RIS+  +G  +S +    L  + K  ++   + E G+ + FE++
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIM 125

[Close]

BLAST of Carg15084 vs. Swiss-Prot
Match: sp|Q12676|FOLD_YEAST (Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FOL3 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 27/64 (42.19%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 81  LGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           L R+ +L+E LGNPQ+  + +HIAGT GKGS   +LS++L+ + Y +G +T+PH+  + +
Sbjct: 7   LSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPHLVHVTD 66

Query: 141 RISL 145
            I++
Sbjct: 67  SITI 70

[Close]

BLAST of Carg15084 vs. Swiss-Prot
Match: sp|Q54CY5|FOLC_DICDI (Putative folylpolyglutamate synthase OS=Dictyostelium discoideum OX=44689 GN=folC PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.4e-10
Identity = 39/104 (37.50%), Postives = 58/104 (55.77%), Query Frame = 0


Query: 74  ESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSP 133
           E    F +  M+   + L     +   IH+AGTKGKGST A   +I+R +G+S G +TSP
Sbjct: 110 EESAKFLMEEMRNYCKTLSIDLERESIIHVAGTKGKGSTCAITESIIREQGFSTGLFTSP 169

Query: 134 HIETIRERISLGRSGEMVSGKALNFLFKRNKELL--DQSVKLEN 176
           H+ + RERI +  +GEM+S +  +  F    +LL  D   +L N
Sbjct: 170 HLISPRERIRI--NGEMISKEMFSQYFWNCWDLLIKDYQTQLPN 211

[Close]

BLAST of Carg15084 vs. Swiss-Prot
Match: sp|P15925|FPGS_LACCA (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.7e-09
Identity = 36/95 (37.89%), Postives = 49/95 (51.58%), Query Frame = 0


Query: 50  TEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGK 109
           TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT GK
Sbjct: 4   TETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGTNGK 63

Query: 110 GSTAAFLSNILRAEGYSVGCYTSPHIETIRERISL 145
           GS A  ++++L A G +VG YTSP I    ERI +
Sbjct: 64  GSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMI 85

[Close]

BLAST of Carg15084 vs. TrEMBL
Match: tr|A0A1S4E568|A0A1S4E568_CUCME (dihydrofolate synthetase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.5e-87
Identity = 162/184 (88.04%), Postives = 172/184 (93.48%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALN LFKRNKE+ DQSV+LE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. TrEMBL
Match: tr|A0A1S3CNR7|A0A1S3CNR7_CUCME (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.5e-87
Identity = 162/184 (88.04%), Postives = 172/184 (93.48%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MTEFMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALN LFKRNKE+ DQSV+LE+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. TrEMBL
Match: tr|A0A0A0L7A4|A0A0A0L7A4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-86
Identity = 158/184 (85.87%), Postives = 171/184 (92.93%), Query Frame = 0


Query: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60
           MN+FKYHHH RPQI G LLLN+F+GE PSISSR GSKQCF  HSEDQ MT+FMEYLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP G+GT+S+DGFDLGRM+RLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALN LFKRNKE+ DQSV+LENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVL 185
           FEVL
Sbjct: 181 FEVL 184

[Close]

BLAST of Carg15084 vs. TrEMBL
Match: tr|A0A2I4H7G0|A0A2I4H7G0_9ROSI (dihydrofolate synthetase isoform X1 OS=Juglans regia OX=51240 GN=LOC109014167 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.5e-60
Identity = 115/152 (75.66%), Postives = 133/152 (87.50%), Query Frame = 0


Query: 33  RIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLG 92
           ++GSK+CF   SED ++ +FMEY+DSLKNYEKLGVP G+GT+SEDGFDLGRM+RLMERLG
Sbjct: 30  QVGSKRCFSTQSEDPELNDFMEYMDSLKNYEKLGVPRGSGTDSEDGFDLGRMRRLMERLG 89

Query: 93  NPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVS 152
           NPQSKF A+HIAGTKGKGSTAAFLSNILRAEGYSVGCY+SPHI+TIRERISLG     VS
Sbjct: 90  NPQSKFMAVHIAGTKGKGSTAAFLSNILRAEGYSVGCYSSPHIQTIRERISLGGFSAPVS 149

Query: 153 GKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
            K LN LF + K+ LDQ+++ ENGRISHFEVL
Sbjct: 150 AKTLNCLFLQFKKTLDQAMERENGRISHFEVL 181

[Close]

BLAST of Carg15084 vs. TrEMBL
Match: tr|A0A2I4H7E2|A0A2I4H7E2_9ROSI (dihydrofolate synthetase isoform X2 OS=Juglans regia OX=51240 GN=LOC109014167 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.5e-60
Identity = 115/152 (75.66%), Postives = 133/152 (87.50%), Query Frame = 0


Query: 33  RIGSKQCFGNHSEDQDMTEFMEYLDSLKNYEKLGVPTGAGTESEDGFDLGRMKRLMERLG 92
           ++GSK+CF   SED ++ +FMEY+DSLKNYEKLGVP G+GT+SEDGFDLGRM+RLMERLG
Sbjct: 30  QVGSKRCFSTQSEDPELNDFMEYMDSLKNYEKLGVPRGSGTDSEDGFDLGRMRRLMERLG 89

Query: 93  NPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVS 152
           NPQSKF A+HIAGTKGKGSTAAFLSNILRAEGYSVGCY+SPHI+TIRERISLG     VS
Sbjct: 90  NPQSKFMAVHIAGTKGKGSTAAFLSNILRAEGYSVGCYSSPHIQTIRERISLGGFSAPVS 149

Query: 153 GKALNFLFKRNKELLDQSVKLENGRISHFEVL 185
            K LN LF + K+ LDQ+++ ENGRISHFEVL
Sbjct: 150 AKTLNCLFLQFKKTLDQAMERENGRISHFEVL 181

The following BLAST results are available for this feature:

BLAST of Carg15084 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922899.1	2.6e-99	98.91	dihydrofolate synthetase isoform X2 [Cucurbita moschata]	[more]
XP_022922901.1	2.6e-99	98.91	dihydrofolate synthetase isoform X4 [Cucurbita moschata]	[more]
XP_022922900.1	2.6e-99	98.91	dihydrofolate synthetase isoform X3 [Cucurbita moschata]	[more]
XP_022922898.1	2.6e-99	98.91	dihydrofolate synthetase isoform X1 [Cucurbita moschata]	[more]
XP_023553540.1	5.7e-99	98.37	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg15084 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G41480.1	2.5e-48	65.25	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	1.8e-09	41.98	DHFS-FPGS homolog D	[more]
AT5G05980.1	5.1e-09	32.14	DHFS-FPGS homolog B	[more]
AT3G10160.1	2.5e-08	52.08	DHFS-FPGS homolog C	[more]

BLAST of Carg15084 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|F4JYE9\|DHFS_ARATH	4.5e-47	65.25	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
sp\|Q05865\|FOLC_BACSU	7.5e-18	44.23	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
sp\|Q12676\|FOLD_YEAST	3.1e-11	42.19	Dihydrofolate synthetase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
sp\|Q54CY5\|FOLC_DICDI	4.4e-10	37.50	Putative folylpolyglutamate synthase OS=Dictyostelium discoideum OX=44689 GN=fol...	[more]
sp\|P15925\|FPGS_LACCA	1.7e-09	37.89	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]

BLAST of Carg15084 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4E568\|A0A1S4E568_CUCME	2.5e-87	88.04	dihydrofolate synthetase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4...	[more]
tr\|A0A1S3CNR7\|A0A1S3CNR7_CUCME	2.5e-87	88.04	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=4...	[more]
tr\|A0A0A0L7A4\|A0A0A0L7A4_CUCSA	1.6e-86	85.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=4 SV=1	[more]
tr\|A0A2I4H7G0\|A0A2I4H7G0_9ROSI	3.5e-60	75.66	dihydrofolate synthetase isoform X1 OS=Juglans regia OX=51240 GN=LOC109014167 PE...	[more]
tr\|A0A2I4H7E2\|A0A2I4H7E2_9ROSI	3.5e-60	75.66	dihydrofolate synthetase isoform X2 OS=Juglans regia OX=51240 GN=LOC109014167 PE...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004326	tetrahydrofolylpolyglutamate synthase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0009396	folic acid-containing compound biosynthetic process
GO:0009058	biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR018109	Folylpolyglutamate_synth_CS
IPR001645	Folylpolyglutamate_synth
IPR036565	Mur-like_cat_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046656	folic acid biosynthetic process
biological_process	GO:0006536	glutamate metabolic process
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15084-RA	Carg15084-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	G3DSA:3.40.1190.10		coord: 38..190 e-value: 4.8E-37 score: 129.5
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	SSF53623	MurD-like peptide ligases, catalytic domain	coord: 77..186
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 43..184
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 43..184
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg15084	Silver-seed gourd	carcarB494
Carg15084	Silver-seed gourd	carcarB503
Carg15084	Cucumber (Gy14) v1	carcgyB1107
Carg15084	Cucumber (Gy14) v2	carcgybB0978
Carg15084	Cucumber (Gy14) v2	carcgybB0977
Carg15084	Cucumber (Gy14) v1	carcgyB1106
Carg15084	Cucurbita maxima (Rimu)	carcmaB1364
Carg15084	Cucurbita maxima (Rimu)	carcmaB1365
Carg15084	Cucurbita maxima (Rimu)	carcmaB1366
Carg15084	Cucurbita maxima (Rimu)	carcmaB1368
Carg15084	Cucurbita maxima (Rimu)	carcmaB1369
Carg15084	Cucurbita moschata (Rifu)	carcmoB1325
Carg15084	Cucurbita moschata (Rifu)	carcmoB1326
Carg15084	Cucurbita moschata (Rifu)	carcmoB1328
Carg15084	Cucurbita moschata (Rifu)	carcmoB1329
Carg15084	Cucurbita pepo (Zucchini)	carcpeB1373
Carg15084	Cucurbita pepo (Zucchini)	carcpeB1375
Carg15084	Cucurbita pepo (Zucchini)	carcpeB1376
Carg15084	Cucurbita pepo (Zucchini)	carcpeB1377
Carg15084	Wild cucumber (PI 183967)	carcpiB1029
Carg15084	Wild cucumber (PI 183967)	carcpiB1025
Carg15084	Cucumber (Chinese Long) v2	carcuB1002
Carg15084	Cucumber (Chinese Long) v2	carcuB1005
Carg15084	Cucumber (Chinese Long) v3	carcucB1033
Carg15084	Cucumber (Chinese Long) v3	carcucB1035
Carg15084	Bottle gourd (USVL1VR-Ls)	carlsiB902
Carg15084	Bottle gourd (USVL1VR-Ls)	carlsiB904
Carg15084	Melon (DHL92) v3.5.1	carmeB0962
Carg15084	Melon (DHL92) v3.5.1	carmeB0966
Carg15084	Melon (DHL92) v3.6.1	carmedB0970
Carg15084	Melon (DHL92) v3.6.1	carmedB0971
Carg15084	Melon (DHL92) v3.6.1	carmedB0975
Carg15084	Watermelon (Charleston Gray)	carwcgB0963
Carg15084	Watermelon (Charleston Gray)	carwcgB0964
Carg15084	Watermelon (Charleston Gray)	carwcgB0965
Carg15084	Watermelon (97103) v1	carwmB0990
Carg15084	Watermelon (97103) v1	carwmB0991
Carg15084	Watermelon (97103) v2	carwmbB1040
Carg15084	Watermelon (97103) v2	carwmbB1041
Carg15084	Watermelon (97103) v2	carwmbB1042
Carg15084	Wax gourd	carwgoB1157
Carg15084	Wax gourd	carwgoB1160