Carg13860-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg13860-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Long-chain-fatty-acid CoA ligase, putative
Location	Cucurbita_argyrosperma_scaffold_042 : 299979 .. 300961 (-)
Sequence length	504

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGTGGCGGTTCCTTTTACTCATGGATTTCTACCATTTCTGGTCATTCGCTCCAAAGAAGCCCTGATTTTGGAATCTCTTGCCAATCGAAGGTAATTTTTGAAATCTGTTTTGAATGTCATTATGTTATGATTTAGAGATAAAATGAATGAATTATAATGTAGAGAGAGAGAGGTTGGAGTTATGGAGGTATTGTCCAATGCTAGAAAGTTGTTTGGTGGAAGGAATTAGTTCCTGATATTTGGAGGTTTTCAGCGGAGAGGTATGGCGATCGGGTAGCGTTGCTGGATCCATACCATAATCCTGCTTCCAAGATTACTTATAAACAGGTATCCATACCATAATCTTGTTAGTATTCCTCGTTTGCTTCTTCAGCATAGTCAGTGATTATGTTTTTGCTTCATTTTAGCAACGAAACACCATCTTGTTTCTTATATTACTTACTTTTCTCTTCTTTTTTTCTTCATAAATTTAAATTTGCAGCTAGAACAAAGTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATGGCTTGTTGCCGATCAAGGTATGCGTTGATTGTATAAAATATTGTATTTCATTTGGTTATATTTTATTAGTTTGGGTTCAGGTATTATGAGTATGGGAGAAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATTGAAAGGTTTCTTAAATTGCTTCATAGGTTTTAACATAAACTATGTTTTGGTTTTAAATTTAAACTTTCACTGTGTAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATCATTGAAACATTCCGTTCGAAGGCATCCATGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGACGGTATTGGACATCATGGATATGGGATGAGAGAGTCGTAA

mRNA sequence

ATGGGCGTGGCGGTTCCTTTTACTCATGGATTTCTACCATTTCTGGTCATTCGCTCCAAAGAAGCCCTGATTTTGGAATCTCTTGCCAATCGAAGAGAGAGAGAGGTTGGAGTTATGGAGGAATTAGTTCCTGATATTTGGAGGTTTTCAGCGGAGAGGTATGGCGATCGGGTAGCGTTGCTGGATCCATACCATAATCCTGCTTCCAAGATTACTTATAAACAGCTAGAACAAAGTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATGGCTTGTTGCCGATCAAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATCATTGAAACATTCCGTTCGAAGGCATCCATGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGACGGTATTGGACATCATGGATATGGGATGAGAGAGTCGTAA

Coding sequence (CDS)

ATGGGCGTGGCGGTTCCTTTTACTCATGGATTTCTACCATTTCTGGTCATTCGCTCCAAAGAAGCCCTGATTTTGGAATCTCTTGCCAATCGAAGAGAGAGAGAGGTTGGAGTTATGGAGGAATTAGTTCCTGATATTTGGAGGTTTTCAGCGGAGAGGTATGGCGATCGGGTAGCGTTGCTGGATCCATACCATAATCCTGCTTCCAAGATTACTTATAAACAGCTAGAACAAAGTATCTTGAATTTCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATTGCACTCTTTTCTGATAATTCATGCCGATGGCTTGTTGCCGATCAAGCATTAGTTGTAGACAACCCTGAATTGTTCAATCGAATCATTGAAACATTCCGTTCGAAGGCATCCATGAGATGTATCATTCTTCTATGGGGCGAAAACTCAAGCCTGGCAGCTGAGGGACTGGACGGTATTGGACATCATGGATATGGGATGAGAGAGTCGTAA

Protein sequence

MGVAVPFTHGFLPFLVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGLDGIGHHGYGMRES

Homology

[Close]

BLAST of Carg13860-RA vs. NCBI nr
Match: XP_022958899.1 (probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 205.3 bits (521), Expect = 1.6e-49
Identity = 111/188 (59.04%), Postives = 121/188 (64.36%), Query Frame = 0


Query: 9   HGFLP---FLVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYH 68
           H  LP    L +R    L+  S        V    + VPDIWR SAERYGDRVAL+DPYH
Sbjct: 6   HDVLPETETLQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYH 65

Query: 69  NPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQ------------ 128
           NPASK+TYK+LEQSILNF EGLRAIGIKPDEKIALF+DNSCRWLVADQ            
Sbjct: 66  NPASKMTYKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQGIMTMGAINVVR 125

Query: 129 -------------------ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGL 163
                              ALVVDNPELFNRI+ETF  KASMRC+ILLWGE SSLA EG+
Sbjct: 126 GSRSSSEELLQIYNHSESVALVVDNPELFNRIVETFHLKASMRCVILLWGEKSSLAHEGM 185

[Close]

BLAST of Carg13860-RA vs. NCBI nr
Match: XP_023006518.1 (probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 205.3 bits (521), Expect = 1.6e-49
Identity = 111/188 (59.04%), Postives = 121/188 (64.36%), Query Frame = 0


Query: 9   HGFLP---FLVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYH 68
           H  LP    L +R    L+  S        V    + VPDIWR SAERYGDRVAL+DPYH
Sbjct: 6   HDVLPETETLQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYH 65

Query: 69  NPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQ------------ 128
           NPASK+TYK+LEQSILNF EGLRAIGIKPDEKIALF+DNSCRWLVADQ            
Sbjct: 66  NPASKMTYKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQGIMTMGAINVVR 125

Query: 129 -------------------ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGL 163
                              ALVVDNPELFNRI+ETF  KASMRC+ILLWGE SSLA EG+
Sbjct: 126 GSRSSSEELLQIYNHSESVALVVDNPELFNRIVETFHLKASMRCVILLWGEKSSLADEGM 185

[Close]

BLAST of Carg13860-RA vs. NCBI nr
Match: XP_023548746.1 (probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 204.9 bits (520), Expect = 2.1e-49
Identity = 111/188 (59.04%), Postives = 121/188 (64.36%), Query Frame = 0


Query: 9   HGFLP---FLVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYH 68
           H  LP    L +R    L+  S        V    + VPDIWR SAERYGDRVAL+DPYH
Sbjct: 6   HDVLPETETLQLRRYSPLLESSFVPGNSATVSDDWQAVPDIWRSSAERYGDRVALVDPYH 65

Query: 69  NPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQ------------ 128
           NPASK+TYK+LEQSILNF EGLRAIGIKPDEKIALF+DNSCRWLVADQ            
Sbjct: 66  NPASKMTYKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQGIMTMGAINVVR 125

Query: 129 -------------------ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGL 163
                              ALVVDNPELFNRI+ETF  KASMRC+ILLWGE SSLA EG+
Sbjct: 126 GSRSSSEELLQIYNHSESVALVVDNPELFNRIVETFHLKASMRCVILLWGEKSSLAHEGM 185

[Close]

BLAST of Carg13860-RA vs. NCBI nr
Match: XP_022958898.1 (probable acyl-activating enzyme 16, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 204.5 bits (519), Expect = 2.8e-49
Identity = 108/179 (60.34%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 15  LVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYHNPASKITYK 74
           L +R    L+  S        V    + VPDIWR SAERYGDRVAL+DPYHNPASK+TYK
Sbjct: 66  LQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYHNPASKMTYK 125

Query: 75  QLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQ--------------------- 134
           +LEQSILNF EGLRAIGIKPDEKIALF+DNSCRWLVADQ                     
Sbjct: 126 ELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQGIMTMGAINVVRGSRSSSEEL 185

Query: 135 ----------ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGLDGIGHHGY 163
                     ALVVDNPELFNRI+ETF  KASMRC+ILLWGE SSLA EG+DGI    Y
Sbjct: 186 LQIYNHSESVALVVDNPELFNRIVETFHLKASMRCVILLWGEKSSLAHEGMDGIAVFDY 244

[Close]

BLAST of Carg13860-RA vs. NCBI nr
Match: XP_023006517.1 (probable acyl-activating enzyme 16, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 204.5 bits (519), Expect = 2.8e-49
Identity = 108/179 (60.34%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 15  LVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYHNPASKITYK 74
           L +R    L+  S        V    + VPDIWR SAERYGDRVAL+DPYHNPASK+TYK
Sbjct: 66  LQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYHNPASKMTYK 125

Query: 75  QLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQ--------------------- 134
           +LEQSILNF EGLRAIGIKPDEKIALF+DNSCRWLVADQ                     
Sbjct: 126 ELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQGIMTMGAINVVRGSRSSSEEL 185

Query: 135 ----------ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGLDGIGHHGY 163
                     ALVVDNPELFNRI+ETF  KASMRC+ILLWGE SSLA EG+DGI    Y
Sbjct: 186 LQIYNHSESVALVVDNPELFNRIVETFHLKASMRCVILLWGEKSSLADEGMDGISVFDY 244

[Close]

BLAST of Carg13860-RA vs. TAIR10
Match: AT4G14070.1 (acyl-activating enzyme 15)

HSP 1 Score: 170.2 bits (430), Expect = 1.0e-42
Identity = 83/143 (58.04%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 43  VPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 102
           VPDIWR SAE+YGDRVAL+DPYH+P  K+TYKQLEQ IL+F EGLR +G+K DEKIALF+
Sbjct: 99  VPDIWRSSAEKYGDRVALVDPYHDPPLKLTYKQLEQEILDFAEGLRVLGVKADEKIALFA 158

Query: 103 DNSCRWLVADQ-------------------------------ALVVDNPELFNRIIETFR 155
           DNSCRWLV+DQ                               A+VVDNPE FNRI E+F 
Sbjct: 159 DNSCRWLVSDQGIMATGAVNVVRGSRSSVEELLQIYRHSESVAIVVDNPEFFNRIAESFT 218

[Close]

BLAST of Carg13860-RA vs. TAIR10
Match: AT3G23790.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 161.8 bits (408), Expect = 3.7e-40
Identity = 85/161 (52.80%), Postives = 101/161 (62.73%), Query Frame = 0


Query: 25  LESLANRREREVGVME-ELVPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNF 84
           LE L+  RE  +   E + VPDIWR S E+YGDRVA++DPYH+P S  TY+QLEQ IL+F
Sbjct: 64  LERLSLPREAALSSNEWKSVPDIWRSSVEKYGDRVAVVDPYHDPPSTFTYRQLEQEILDF 123

Query: 85  CEGLRAIGIKPDEKIALFSDNSCRWLVADQ------------------------------ 144
            EGLR +G+K DEKIALF+DNSCRWLVADQ                              
Sbjct: 124 VEGLRVVGVKADEKIALFADNSCRWLVADQGIMATGAVNVVRGSRSSVEELLQIYCHSES 183

Query: 145 -ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEG 154
            ALVVDNPE FNRI E+F  KA+ + +ILLWGE SSL   G
Sbjct: 184 VALVVDNPEFFNRIAESFSYKAAPKFVILLWGEKSSLVTAG 224

[Close]

BLAST of Carg13860-RA vs. TAIR10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 43.1 bits (100), Expect = 1.9e-04
Identity = 22/75 (29.33%), Postives = 39/75 (52.00%), Query Frame = 0


Query: 45  DIWRFSAERYGDRVAL--LDPYHNPASKI---TYKQLEQSILNFCEGLRAIGIKPDEKIA 104
           DI+R + E+Y +   L   +  +  A K    TYK++   ++     LR+ GIK  EK  
Sbjct: 49  DIFRTAVEKYPNNRMLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCG 108

Query: 105 LFSDNSCRWLVADQA 115
           ++  N C W+++ +A
Sbjct: 109 IYGINCCEWIISMEA 123

[Close]

BLAST of Carg13860-RA vs. Swiss-Prot
Match: sp|Q8W471|AAE15_ARATH (Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AAE15 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.9e-41
Identity = 83/143 (58.04%), Postives = 98/143 (68.53%), Query Frame = 0


Query: 43  VPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 102
           VPDIWR SAE+YGDRVAL+DPYH+P  K+TYKQLEQ IL+F EGLR +G+K DEKIALF+
Sbjct: 99  VPDIWRSSAEKYGDRVALVDPYHDPPLKLTYKQLEQEILDFAEGLRVLGVKADEKIALFA 158

Query: 103 DNSCRWLVADQ-------------------------------ALVVDNPELFNRIIETFR 155
           DNSCRWLV+DQ                               A+VVDNPE FNRI E+F 
Sbjct: 159 DNSCRWLVSDQGIMATGAVNVVRGSRSSVEELLQIYRHSESVAIVVDNPEFFNRIAESFT 218

[Close]

BLAST of Carg13860-RA vs. Swiss-Prot
Match: sp|Q9LK39|AAE16_ARATH (Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AAE16 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 6.7e-39
Identity = 85/161 (52.80%), Postives = 101/161 (62.73%), Query Frame = 0


Query: 25  LESLANRREREVGVME-ELVPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNF 84
           LE L+  RE  +   E + VPDIWR S E+YGDRVA++DPYH+P S  TY+QLEQ IL+F
Sbjct: 64  LERLSLPREAALSSNEWKSVPDIWRSSVEKYGDRVAVVDPYHDPPSTFTYRQLEQEILDF 123

Query: 85  CEGLRAIGIKPDEKIALFSDNSCRWLVADQ------------------------------ 144
            EGLR +G+K DEKIALF+DNSCRWLVADQ                              
Sbjct: 124 VEGLRVVGVKADEKIALFADNSCRWLVADQGIMATGAVNVVRGSRSSVEELLQIYCHSES 183

Query: 145 -ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEG 154
            ALVVDNPE FNRI E+F  KA+ + +ILLWGE SSL   G
Sbjct: 184 VALVVDNPEFFNRIAESFSYKAAPKFVILLWGEKSSLVTAG 224

[Close]

BLAST of Carg13860-RA vs. Swiss-Prot
Match: sp|Q70LM7|LGRA_BREPA (Linear gramicidin synthase subunit A OS=Brevibacillus parabrevis OX=54914 GN=lgrA PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.4e-04
Identity = 26/97 (26.80%), Postives = 47/97 (48.45%), Query Frame = 0


Query: 20  KEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQS 79
           +E L L+ L    +R    +++    ++    E   D VA++D        +TYKQL + 
Sbjct: 181 RELLALKRLCAEPKRGEKPIDKTFHQLFEQQVEMTPDHVAVVD----RGQSLTYKQLNER 240

Query: 80  ILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQALV 117
                  LR  G+KPD+++A+  D S   +V+  A++
Sbjct: 241 ANQLAHHLRGKGVKPDDQVAIMLDKSLDMIVSILAVM 273

[Close]

BLAST of Carg13860-RA vs. TrEMBL
Match: tr|A0A1S3C750|A0A1S3C750_CUCME (probable acyl-activating enzyme 16, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497672 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.4e-48
Identity = 105/174 (60.34%), Postives = 116/174 (66.67%), Query Frame = 0


Query: 15  LVIRSKEALILESLANRREREVGVMEELVPDIWRFSAERYGDRVALLDPYHNPASKITYK 74
           L +R    L+  S        V    + VPDIWR SAERYGDR+AL+DPYHNPASK+TYK
Sbjct: 63  LQLRRYSPLLESSFVPGTSATVSDEWQAVPDIWRSSAERYGDRIALVDPYHNPASKMTYK 122

Query: 75  QLEQSILNFCEGLRAIGIKPDEKIALFSDNSCRWLVADQ--------------------- 134
           +LEQSILNF EGLRAIGIKPDEKIALF+DNSCRWLVADQ                     
Sbjct: 123 ELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQGIMTMGAINVVRGSRSSSEEL 182

Query: 135 ----------ALVVDNPELFNRIIETFRSKASMRCIILLWGENSSLAAEGLDGI 158
                     ALVVDNPELFNRI+ETF  KASMRC+ILLWGE SSLA EG+D I
Sbjct: 183 LQIYNHSESVALVVDNPELFNRILETFHLKASMRCVILLWGEKSSLANEGVDDI 236

[Close]

BLAST of Carg13860-RA vs. TrEMBL
Match: tr|A0A0A0KA45|A0A0A0KA45_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G007970 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 4.5e-48
Identity = 99/148 (66.89%), Postives = 108/148 (72.97%), Query Frame = 0


Query: 41  ELVPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIAL 100
           + VPDIWR SAERYGDR+AL+DPYHNPASK+TYK+LEQSILNF EGLR IGIKPDEKIAL
Sbjct: 89  QAVPDIWRSSAERYGDRIALVDPYHNPASKMTYKELEQSILNFSEGLRVIGIKPDEKIAL 148

Query: 101 FSDNSCRWLVADQ-------------------------------ALVVDNPELFNRIIET 158
           F+DNSCRWLVADQ                               ALVVDNPELFNRI+ET
Sbjct: 149 FADNSCRWLVADQGIMTMGAINVVRGSRSSSEELLQIYNHSESVALVVDNPELFNRIVET 208

[Close]

BLAST of Carg13860-RA vs. TrEMBL
Match: tr|A0A2P5FCI4|A0A2P5FCI4_9ROSA (2,3-dihydroxybenzoate-AMP ligase OS=Trema orientalis OX=63057 GN=TorRG33x02_086340 PE=4 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.1e-41
Identity = 85/151 (56.29%), Postives = 103/151 (68.21%), Query Frame = 0


Query: 43  VPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 102
           VPDIWR SAE+YGDR+A++DPYH+P   +TYKQLEQ IL+F EGLR IGIKPDEK+ALF+
Sbjct: 93  VPDIWRTSAEKYGDRIAVMDPYHDPPLNLTYKQLEQEILDFSEGLRVIGIKPDEKLALFA 152

Query: 103 DNSCRWLVADQ-------------------------------ALVVDNPELFNRIIETFR 162
           DNSCRWLVADQ                                L VD+PELFNRI ETFR
Sbjct: 153 DNSCRWLVADQGIMTTGAINVVRGSRSSVEELLQIYSHSESVGLAVDSPELFNRISETFR 212

[Close]

BLAST of Carg13860-RA vs. TrEMBL
Match: tr|A0A218WW65|A0A218WW65_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr004951 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-40
Identity = 84/151 (55.63%), Postives = 103/151 (68.21%), Query Frame = 0


Query: 43  VPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 102
           VPDIWR SAE+YGDR+AL+DPYH+P S +TYKQLEQ ILNF EGLR +G+KP+EK+ALF+
Sbjct: 97  VPDIWRSSAEKYGDRIALVDPYHDPPSNMTYKQLEQEILNFSEGLRVVGVKPEEKLALFA 156

Query: 103 DNSCRWLVADQ-------------------------------ALVVDNPELFNRIIETFR 162
           DNSCRWLV+DQ                               ALVVDNPELFNR+ E F 
Sbjct: 157 DNSCRWLVSDQGIMATGAINVVRGSRSSVEELLQIYQHSESVALVVDNPELFNRMAEVFF 216

[Close]

BLAST of Carg13860-RA vs. TrEMBL
Match: tr|A0A2I0LD95|A0A2I0LD95_PUNGR (Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_001421 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-40
Identity = 84/151 (55.63%), Postives = 103/151 (68.21%), Query Frame = 0


Query: 43  VPDIWRFSAERYGDRVALLDPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFS 102
           VPDIWR SAE+YGDR+AL+DPYH+P S +TYKQLEQ ILNF EGLR +G+KP+EK+ALF+
Sbjct: 48  VPDIWRSSAEKYGDRIALVDPYHDPPSNMTYKQLEQEILNFSEGLRVVGVKPEEKLALFA 107

Query: 103 DNSCRWLVADQ-------------------------------ALVVDNPELFNRIIETFR 162
           DNSCRWLV+DQ                               ALVVDNPELFNR+ E F 
Sbjct: 108 DNSCRWLVSDQGIMATGAINVVRGSRSSVEELLQIYQHSESVALVVDNPELFNRMAEVFF 167

The following BLAST results are available for this feature:

BLAST of Carg13860-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958899.1	1.6e-49	59.04	probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita moschata...	[more]
XP_023006518.1	1.6e-49	59.04	probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita maxima]	[more]
XP_023548746.1	2.1e-49	59.04	probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita pepo sub...	[more]
XP_022958898.1	2.8e-49	60.34	probable acyl-activating enzyme 16, chloroplastic isoform X1 [Cucurbita moschata...	[more]
XP_023006517.1	2.8e-49	60.34	probable acyl-activating enzyme 16, chloroplastic isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg13860-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14070.1	1.0e-42	58.04	acyl-activating enzyme 15	[more]
AT3G23790.1	3.7e-40	52.80	AMP-dependent synthetase and ligase family protein	[more]
AT4G11030.1	1.9e-04	29.33	AMP-dependent synthetase and ligase family protein	[more]

BLAST of Carg13860-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8W471\|AAE15_ARATH	1.9e-41	58.04	Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Ara...	[more]
sp\|Q9LK39\|AAE16_ARATH	6.7e-39	52.80	Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
sp\|Q70LM7\|LGRA_BREPA	5.4e-04	26.80	Linear gramicidin synthase subunit A OS=Brevibacillus parabrevis OX=54914 GN=lgr...	[more]

BLAST of Carg13860-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C750\|A0A1S3C750_CUCME	3.4e-48	60.34	probable acyl-activating enzyme 16, chloroplastic OS=Cucumis melo OX=3656 GN=LOC...	[more]
tr\|A0A0A0KA45\|A0A0A0KA45_CUCSA	4.5e-48	66.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G007970 PE=4 SV=1	[more]
tr\|A0A2P5FCI4\|A0A2P5FCI4_9ROSA	9.1e-41	56.29	2,3-dihydroxybenzoate-AMP ligase OS=Trema orientalis OX=63057 GN=TorRG33x02_0863...	[more]
tr\|A0A218WW65\|A0A218WW65_PUNGR	1.5e-40	55.63	Uncharacterized protein OS=Punica granatum OX=22663 GN=CDL15_Pgr004951 PE=4 SV=1	[more]
tr\|A0A2I0LD95\|A0A2I0LD95_PUNGR	1.5e-40	55.63	Uncharacterized protein OS=Punica granatum OX=22663 GN=CRG98_001421 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR000873	AMP-dep_Synth/Lig

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg13860	Carg13860	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg13860-RA	Carg13860-RA-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg13860-RA.CDS.5	Carg13860-RA.CDS.5	CDS
Carg13860-RA.CDS.4	Carg13860-RA.CDS.4	CDS
Carg13860-RA.CDS.3	Carg13860-RA.CDS.3	CDS
Carg13860-RA.CDS.2	Carg13860-RA.CDS.2	CDS
Carg13860-RA.CDS.1	Carg13860-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg13860-RA.exon.5	Carg13860-RA.exon.5	exon
Carg13860-RA.exon.4	Carg13860-RA.exon.4	exon
Carg13860-RA.exon.3	Carg13860-RA.exon.3	exon
Carg13860-RA.exon.2	Carg13860-RA.exon.2	exon
Carg13860-RA.exon.1	Carg13860-RA.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.40.50.980		coord: 39..117 e-value: 8.4E-11 score: 43.7
None	No IPR available	PANTHER	PTHR43813:SF2	LONG-CHAIN-FATTY-ACID-- ACYL-CARRIER-PROTEIN LIGASE AEE15, CHLOROPLASTIC-RELATED	coord: 114..155
None	No IPR available	PANTHER	PTHR43813:SF2	LONG-CHAIN-FATTY-ACID-- ACYL-CARRIER-PROTEIN LIGASE AEE15, CHLOROPLASTIC-RELATED	coord: 25..115
None	No IPR available	PANTHER	PTHR43813	FAMILY NOT NAMED	coord: 25..115
None	No IPR available	PANTHER	PTHR43813	FAMILY NOT NAMED	coord: 114..155
None	No IPR available	SUPERFAMILY	SSF56801	Acetyl-CoA synthetase-like	coord: 41..115
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 50..115 e-value: 1.1E-11 score: 43.9