Carg12349-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12349-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	chitinase 2-like
Location	Cucurbita_argyrosperma_scaffold_074 : 448718 .. 450286 (+)
Sequence length	921

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGGATGGAATAATTGTTGTTCTTCTATTGATTCTGGTGGCTCCGCCGTCTAACACGGCGGTGGTAGTACCAGCGACAGTGTTTAGAGAATACATAGGAGCTGAGTTCAATGATGTCAAATTTTCCGACGTTCCGATTAACCCAAATGTAGAAGAGTTTCACTTTCTCCTGTCGTTTGCAATAGACTTTGACAGCTCCCGCCGTTGTTTCACTAATGGAGTATTCAACGTTTTCTGGGATTCCTATAATCTTAGCCCTTCCGCTGTTGCTGCCATTAAAAAGGTAATCAAACGTTCAACAACCCAATATTATACCTAAAAAAAATATATCCTTTTTTGAGGATAAACTCTCGTAGCTTTGTTTTTTGTTTCTTCGAAAGATCTCGTACCAATGGAGATACTCTTATTTGTTGGATGATGAAAGTTTCACGTCCACTAATTTAGAGAATGATTCTGAGTTTCTAATCAATGAATACTCTTTCCATTGGTACGAGGTCTTTTGGGAAGCCCAAAGCAAAGCCACTAGAGCTTATGCTTAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATTATTTACTTATAAACTCACGATCTTCCTCTTAATTAGCTAATGTGGGACTACTCTTTTAATAATCCTCCCCTCGAGCAAAATACACCATAGAGCCTCCCCCTGAGGCCTATGAAGCCCTCGAACAAAGTACATCATTTGTTCGACACTCGAGTCACTTTTGACTCTAATACTATGTTAGGAATCACGACTCTCCACAATAATATGATATTGTCTACTTTGAGTATAAGCTCTCGTAGGTTTGTTTTTCGTTTCTCCAAAAAGTCTCGTGTCGTTAGAGATAGTATTCCTTACTTATAAACTCATAATTGTTCTCTTAATTACCGAACGAAATTACTCGTGCTTTAGGATCATTCAAACGTGAGAGTGGGTGTAAGTCTCGGAGGAGACAGCGTGGGAGACTCAGGCTTTGTTTATTTCAAACCTTCTTCAATAGCTTCATGGGTTTCCAACGCAGAACGTTCACTCACAAAGCTCATAAACACCTTCAATCTCGACGGCATCGACATCGATTACGAACACTTCGAGTCGGACCCCAACACATTCGCTGAGTGCATAGGGCAGCTAATTAGACGGCTAAAGAAGAAGGGAGTCATCTCATTTGCTTCCATTGCCCCTTTTGACGACGCTGAAGTTCAAACCCATTACTTAGCCTTATGGAAAAGATACGGGCACTTGATAGATTATGTTAATTTTCAATTCTATGCTTATGATAAGACCACCACAGTTCATCAGTTTATCAAACATTTCAAAGCTCAAAGCTCAAAGTATGAAGATGGTAGGATCCTGGCAAGCTTAGCCAGCGACGATAGCGGCGGATTGTTGCCAAAGCATGGGTTTTTTAAGGCGTGTCGAAGGCTTAGAACGGACAAACTCCTTCATGGAATCTTTATTTGGTCTGCAGATGATTCACAAGCTCGTGGTTTTAGGTATGAAAAGAAGGCACAAGCACTCCTACAAAGCCCCAACTACCACTATTGA

mRNA sequence

ATGGAAATGGATGGAATAATTGTTGTTCTTCTATTGATTCTGGTGGCTCCGCCGTCTAACACGGCGGTGGTAGTACCAGCGACAGTGTTTAGAGAATACATAGGAGCTGAGTTCAATGATGTCAAATTTTCCGACGTTCCGATTAACCCAAATGTAGAAGAGTTTCACTTTCTCCTGTCGTTTGCAATAGACTTTGACAGCTCCCGCCGTTGTTTCACTAATGGAGTATTCAACGTTTTCTGGGATTCCTATAATCTTAGCCCTTCCGCTGTTGCTGCCATTAAAAAGGATCATTCAAACGTGAGAGTGGGTGTAAGTCTCGGAGGAGACAGCGTGGGAGACTCAGGCTTTGTTTATTTCAAACCTTCTTCAATAGCTTCATGGGTTTCCAACGCAGAACGTTCACTCACAAAGCTCATAAACACCTTCAATCTCGACGGCATCGACATCGATTACGAACACTTCGAGTCGGACCCCAACACATTCGCTGAGTGCATAGGGCAGCTAATTAGACGGCTAAAGAAGAAGGGAGTCATCTCATTTGCTTCCATTGCCCCTTTTGACGACGCTGAAGTTCAAACCCATTACTTAGCCTTATGGAAAAGATACGGGCACTTGATAGATTATGTTAATTTTCAATTCTATGCTTATGATAAGACCACCACAGTTCATCAGTTTATCAAACATTTCAAAGCTCAAAGCTCAAAGTATGAAGATGGTAGGATCCTGGCAAGCTTAGCCAGCGACGATAGCGGCGGATTGTTGCCAAAGCATGGGTTTTTTAAGGCGTGTCGAAGGCTTAGAACGGACAAACTCCTTCATGGAATCTTTATTTGGTCTGCAGATGATTCACAAGCTCGTGGTTTTAGGTATGAAAAGAAGGCACAAGCACTCCTACAAAGCCCCAACTACCACTATTGA

Coding sequence (CDS)

ATGGAAATGGATGGAATAATTGTTGTTCTTCTATTGATTCTGGTGGCTCCGCCGTCTAACACGGCGGTGGTAGTACCAGCGACAGTGTTTAGAGAATACATAGGAGCTGAGTTCAATGATGTCAAATTTTCCGACGTTCCGATTAACCCAAATGTAGAAGAGTTTCACTTTCTCCTGTCGTTTGCAATAGACTTTGACAGCTCCCGCCGTTGTTTCACTAATGGAGTATTCAACGTTTTCTGGGATTCCTATAATCTTAGCCCTTCCGCTGTTGCTGCCATTAAAAAGGATCATTCAAACGTGAGAGTGGGTGTAAGTCTCGGAGGAGACAGCGTGGGAGACTCAGGCTTTGTTTATTTCAAACCTTCTTCAATAGCTTCATGGGTTTCCAACGCAGAACGTTCACTCACAAAGCTCATAAACACCTTCAATCTCGACGGCATCGACATCGATTACGAACACTTCGAGTCGGACCCCAACACATTCGCTGAGTGCATAGGGCAGCTAATTAGACGGCTAAAGAAGAAGGGAGTCATCTCATTTGCTTCCATTGCCCCTTTTGACGACGCTGAAGTTCAAACCCATTACTTAGCCTTATGGAAAAGATACGGGCACTTGATAGATTATGTTAATTTTCAATTCTATGCTTATGATAAGACCACCACAGTTCATCAGTTTATCAAACATTTCAAAGCTCAAAGCTCAAAGTATGAAGATGGTAGGATCCTGGCAAGCTTAGCCAGCGACGATAGCGGCGGATTGTTGCCAAAGCATGGGTTTTTTAAGGCGTGTCGAAGGCTTAGAACGGACAAACTCCTTCATGGAATCTTTATTTGGTCTGCAGATGATTCACAAGCTCGTGGTTTTAGGTATGAAAAGAAGGCACAAGCACTCCTACAAAGCCCCAACTACCACTATTGA

Protein sequence

MEMDGIIVVLLLILVAPPSNTAVVVPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPNYHY

Homology

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BLAST of Carg12349-RA vs. NCBI nr
Match: XP_023524573.1 (chitinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 565.8 bits (1457), Expect = 8.6e-158
Identity = 296/305 (97.05%), Postives = 298/305 (97.70%), Query Frame = 0


Query: 1   MEMDGIXXXXXXXXXXXXXXXXXXXPATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS 60
           MEMDGIXXXXXXXXXXXXXXXXXXX ATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS
Sbjct: 1   MEMDGIXXXXXXXXXXXXXXXXXXXXATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLS 60

Query: 61  FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYF 120
           FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKK+HSNVRVGVSLGGDSVGDSGFVYF
Sbjct: 61  FAIDFDSSRRCFTNGVFNVFWDSYNLSPSAVAAIKKNHSNVRVGVSLGGDSVGDSGFVYF 120

Query: 121 KPSSIASWVSNAERSLTKLINTFNLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS 180
           KPSSIASWVSNAERSLTKLINTF LDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS
Sbjct: 121 KPSSIASWVSNAERSLTKLINTFKLDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVIS 180

Query: 181 FASIAPFDDAEVQTHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDG 240
           FASIAPFDD EVQ HYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSS YE G
Sbjct: 181 FASIAPFDDTEVQAHYLALWKRYGHLIDYVNFQFYAYDKTTTVHQFIKHFKAQSSNYEGG 240

Query: 241 RILASLASDDSGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQ 300
           RILASLASDD+GGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQA GFRYEKKAQALLQ
Sbjct: 241 RILASLASDDTGGLLPKHGFFKACRRLRTDKLLHGIFIWSADDSQAHGFRYEKKAQALLQ 300

Query: 301 SPNYH 306
           SPNYH
Sbjct: 301 SPNYH 305

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BLAST of Carg12349-RA vs. NCBI nr
Match: XP_022949055.1 (chitinase 2-like [Cucurbita moschata])

HSP 1 Score: 565.5 bits (1456), Expect = 1.1e-157
Identity = 274/278 (98.56%), Postives = 274/278 (98.56%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP 88
           VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP
Sbjct: 27  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP 86

Query: 89  SAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGI 148
           SAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGI
Sbjct: 87  SAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGI 146

Query: 149 DIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLID 208
           DIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLID
Sbjct: 147 DIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLID 206

Query: 209 YVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRLR 268
           YVNFQFYAYDKTTTVHQFIKHFKAQSS YE GRILASLASDDSGGLL KHGFFKACRRLR
Sbjct: 207 YVNFQFYAYDKTTTVHQFIKHFKAQSSNYEGGRILASLASDDSGGLLSKHGFFKACRRLR 266

Query: 269 TDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPNYHY 307
           TDKLLHGIFIWSADDSQA GFRYEKKAQALLQSPNYHY
Sbjct: 267 TDKLLHGIFIWSADDSQAHGFRYEKKAQALLQSPNYHY 304

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BLAST of Carg12349-RA vs. NCBI nr
Match: XP_004152902.1 (PREDICTED: chitinase 2-like [Cucumis sativus] >KGN65683.1 hypothetical protein Csa_1G496300 [Cucumis sativus])

HSP 1 Score: 477.2 bits (1227), Expect = 4.0e-131
Identity = 228/278 (82.01%), Postives = 253/278 (91.01%), Query Frame = 0


Query: 26  PATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYN 85
           PA VFREYIGAEFN+VKFSDVPI+ NVEEFHFLLSFAIDFD+S R FTNG F+VFWDS N
Sbjct: 33  PANVFREYIGAEFNNVKFSDVPIHSNVEEFHFLLSFAIDFDASSRSFTNGAFDVFWDSDN 92

Query: 86  LSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNL 145
           L+PSAVA+IKKDHSNVRVGVSLGGD+V + G V+FKPSSI SWVSNAE SLTK+I T+NL
Sbjct: 93  LNPSAVASIKKDHSNVRVGVSLGGDTVDNGGSVFFKPSSIHSWVSNAEHSLTKIIKTYNL 152

Query: 146 DGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGH 205
           DGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAPFDD EVQ+HYLALWKRYGH
Sbjct: 153 DGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAPFDDDEVQSHYLALWKRYGH 212

Query: 206 LIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACR 265
           LIDYVNFQFYAYDK TT+ QFI+HFK Q S YE GRILASL SDDSGGL+PK+GFFKACR
Sbjct: 213 LIDYVNFQFYAYDKGTTIGQFIQHFKDQMSNYEGGRILASLVSDDSGGLVPKNGFFKACR 272

Query: 266 RLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           RL+ +KLL+GIFIWSADDSQA+GF YEK AQ+LL  PN
Sbjct: 273 RLKKEKLLNGIFIWSADDSQAKGFDYEKTAQSLLAKPN 310

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BLAST of Carg12349-RA vs. NCBI nr
Match: XP_008457518.1 (PREDICTED: chitinase 2-like [Cucumis melo])

HSP 1 Score: 470.7 bits (1210), Expect = 3.8e-129
Identity = 225/279 (80.65%), Postives = 255/279 (91.40%), Query Frame = 0


Query: 26  PATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYN 85
           PA VFREYIGAEFN+VKFSDVPI  N+EEFHFLLSFAIDFD+S R FTNG F+VFWDS N
Sbjct: 33  PANVFREYIGAEFNNVKFSDVPIRSNIEEFHFLLSFAIDFDASSRSFTNGAFDVFWDSDN 92

Query: 86  LSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNL 145
           L+PSAVA+IKKDHSNVRVGVSLGGD+V + GFV+FKPSSI SWVSNAE SLTK+INT+NL
Sbjct: 93  LNPSAVASIKKDHSNVRVGVSLGGDTVDNGGFVFFKPSSIHSWVSNAEHSLTKIINTYNL 152

Query: 146 DGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGH 205
           DGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAPFDD EVQ+HYLALW+ YGH
Sbjct: 153 DGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAPFDDDEVQSHYLALWRSYGH 212

Query: 206 LIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDS-GGLLPKHGFFKAC 265
           LIDYVNFQFYAYDK TTV QFI+HFK Q S YE GR+LASLASD+S GGL+P++GFFKAC
Sbjct: 213 LIDYVNFQFYAYDKGTTVGQFIRHFKDQVSNYEGGRVLASLASDNSAGGLVPENGFFKAC 272

Query: 266 RRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           RRL+ +KLL GIFIWSADDS+A+GF YEK+AQ+LL +PN
Sbjct: 273 RRLKREKLLDGIFIWSADDSKAKGFGYEKEAQSLLATPN 311

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BLAST of Carg12349-RA vs. NCBI nr
Match: XP_022156744.1 (chitinase 2-like [Momordica charantia])

HSP 1 Score: 468.0 bits (1203), Expect = 2.5e-128
Identity = 226/277 (81.59%), Postives = 249/277 (89.89%), Query Frame = 0


Query: 27  ATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNL 86
           A +FREYIGAEF DVKFSDVPINP+V EFHFLLSFAIDFD+SRR FTNG+FNVFWDS+NL
Sbjct: 29  AKLFREYIGAEFKDVKFSDVPINPDV-EFHFLLSFAIDFDASRRSFTNGIFNVFWDSHNL 88

Query: 87  SPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLD 146
           +PS V AIKKDHSNV+VG+S+GGD+V  +GF YFKPSSI SWVSNAERSLTKLIN FNLD
Sbjct: 89  NPSDVVAIKKDHSNVKVGLSIGGDTV-PNGFAYFKPSSIDSWVSNAERSLTKLINAFNLD 148

Query: 147 GIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHL 206
            IDIDYEHF+SDPNTFAECIG+LI+RLKKKG ISFASIAPFD AEVQ+HYLALW+ YGHL
Sbjct: 149 SIDIDYEHFKSDPNTFAECIGRLIKRLKKKGAISFASIAPFDKAEVQSHYLALWRSYGHL 208

Query: 207 IDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRR 266
           IDYVNFQFYAYDKTTTV QFI+HFKAQ+S YE GR+LASL SDDSGGLL ++GFF ACRR
Sbjct: 209 IDYVNFQFYAYDKTTTVPQFIQHFKAQTSNYEGGRVLASLVSDDSGGLLAENGFFTACRR 268

Query: 267 LRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           LR +KLLHGIF+WSADDS A GF YEKKAQ LL  PN
Sbjct: 269 LRAEKLLHGIFVWSADDSIANGFGYEKKAQELLAMPN 303

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BLAST of Carg12349-RA vs. Swiss-Prot
Match: sp|Q9SLP4|CHIT1_TULBA (Chitinase 1 OS=Tulipa bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.1e-101
Identity = 179/280 (63.93%), Postives = 220/280 (78.57%), Query Frame = 0


Query: 27  ATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDS-SRRCFTNGVFNVFWDSYN 86
           A VFREYIG++FNDVKFSDVPINP+V +FHF+L+FAID+ S S    TNG F  FWD+ N
Sbjct: 26  ALVFREYIGSQFNDVKFSDVPINPDV-DFHFILAFAIDYTSGSSPTPTNGNFKPFWDTNN 85

Query: 87  LSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNL 146
           LSPS VAA+K+ HSNV+V +SLGGDSVG    V+F PSS++SWV NA  SLT++I  ++L
Sbjct: 86  LSPSQVAAVKRTHSNVKVSLSLGGDSVGGKN-VFFSPSSVSSWVENAVSSLTRIIKQYHL 145

Query: 147 DGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGH 206
           DGIDIDYEHF+ DPNTFAECIGQL+ RLKK  V+SF SIAPFDDA+VQ+HY ALW++YGH
Sbjct: 146 DGIDIDYEHFKGDPNTFAECIGQLVTRLKKNEVVSFVSIAPFDDAQVQSHYQALWEKYGH 205

Query: 207 LIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACR 266
            IDYVNFQFYAY   T+V QF+K+F+ QSS Y  G++L S ++D SGGL P +GFF+AC 
Sbjct: 206 QIDYVNFQFYAYSARTSVEQFLKYFEEQSSNYHGGKVLVSFSTDSSGGLKPDNGFFRACS 265

Query: 267 RLRTDKLLHGIFIWSADDSQARG--FRYEKKAQALLQSPN 304
            L+    LHGIF+WSADDS      FRYE +AQ++L S N
Sbjct: 266 ILKKQGKLHGIFVWSADDSLMSNNVFRYEMQAQSMLASVN 303

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BLAST of Carg12349-RA vs. Swiss-Prot
Match: sp|Q7M443|CHIT2_TULBA (Chitinase 2 OS=Tulipa bakeri OX=110455 PE=1 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-99
Identity = 177/276 (64.13%), Postives = 218/276 (78.99%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDS-SRRCFTNGVFNVFWDSYNLS 88
           +FREYIGA+FNDVKFSDVPINPNV +FHF+L+FAID+ S S    TNG FN FWD+ NLS
Sbjct: 2   LFREYIGAQFNDVKFSDVPINPNV-DFHFILAFAIDYTSGSSPTPTNGNFNPFWDTNNLS 61

Query: 89  PSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDG 148
           PS VAAIK+ ++NV+V VSLGG+SVG    V+F PSS++SWV NA  SLTK+I  ++LDG
Sbjct: 62  PSQVAAIKRTYNNVKVSVSLGGNSVGGER-VFFNPSSVSSWVDNAVSSLTKIIKQYHLDG 121

Query: 149 IDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLI 208
           IDIDYEHF+ DPNTFAECIGQL+ RLKK GV+SF SIAPFDDA+VQ+HY ALW++YGH I
Sbjct: 122 IDIDYEHFKGDPNTFAECIGQLVTRLKKNGVVSFVSIAPFDDAQVQSHYQALWEKYGHQI 181

Query: 209 DYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRL 268
           DYVNFQFY Y    +V QF+K+F+ Q S Y  G++L S ++D+SGGL P++GFF AC  L
Sbjct: 182 DYVNFQFYVYSSRMSVEQFLKYFEMQRSNYPGGKVLVSFSTDNSGGLKPRNGFFDACSIL 241

Query: 269 RTDKLLHGIFIWSADDSQARG--FRYEKKAQALLQS 302
           +    LHGIF+WSADDS      F+YE +AQ+LL S
Sbjct: 242 KKQGKLHGIFVWSADDSLMSNDVFKYEMQAQSLLAS 275

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BLAST of Carg12349-RA vs. Swiss-Prot
Match: sp|P39657|RUAP_SOYBN (RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 6.8e-21
Identity = 65/188 (34.57%), Postives = 93/188 (49.47%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP 88
           VFRE+     +D  F +  I  N+ EF   LS A D+D +    TNG F  +WD+  ++P
Sbjct: 7   VFREFT----SDDSFLNQVIPENITEFQVTLSLARDYDGNNS--TNGKFIPYWDTEKVTP 66

Query: 89  SAVAAIKKDHS----NVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFN 148
             +   KK +      V+V VS+G  +     F     S+  +WVS A  SL  +I T+N
Sbjct: 67  EVIKKFKKKYEPTALRVKVLVSIGNKN-KQFPFTIGSDSNSEAWVSEATASLKSIIKTYN 126

Query: 149 LDGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYG 208
           LDGID+ YE   ++   F   +G L+R LK+  +I+ AS A   DA     Y  L+  Y 
Sbjct: 127 LDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNLLYAEYA 186

Query: 209 HLIDYVNF 213
              D V F
Sbjct: 187 TFFDTVVF 187

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BLAST of Carg12349-RA vs. TrEMBL
Match: tr|A0A0A0LV90|A0A0A0LV90_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G496300 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 2.7e-131
Identity = 228/278 (82.01%), Postives = 253/278 (91.01%), Query Frame = 0


Query: 26  PATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYN 85
           PA VFREYIGAEFN+VKFSDVPI+ NVEEFHFLLSFAIDFD+S R FTNG F+VFWDS N
Sbjct: 33  PANVFREYIGAEFNNVKFSDVPIHSNVEEFHFLLSFAIDFDASSRSFTNGAFDVFWDSDN 92

Query: 86  LSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNL 145
           L+PSAVA+IKKDHSNVRVGVSLGGD+V + G V+FKPSSI SWVSNAE SLTK+I T+NL
Sbjct: 93  LNPSAVASIKKDHSNVRVGVSLGGDTVDNGGSVFFKPSSIHSWVSNAEHSLTKIIKTYNL 152

Query: 146 DGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGH 205
           DGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAPFDD EVQ+HYLALWKRYGH
Sbjct: 153 DGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAPFDDDEVQSHYLALWKRYGH 212

Query: 206 LIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACR 265
           LIDYVNFQFYAYDK TT+ QFI+HFK Q S YE GRILASL SDDSGGL+PK+GFFKACR
Sbjct: 213 LIDYVNFQFYAYDKGTTIGQFIQHFKDQMSNYEGGRILASLVSDDSGGLVPKNGFFKACR 272

Query: 266 RLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           RL+ +KLL+GIFIWSADDSQA+GF YEK AQ+LL  PN
Sbjct: 273 RLKKEKLLNGIFIWSADDSQAKGFDYEKTAQSLLAKPN 310

[Close]

BLAST of Carg12349-RA vs. TrEMBL
Match: tr|A0A1S3C5A3|A0A1S3C5A3_CUCME (chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103497192 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.5e-129
Identity = 225/279 (80.65%), Postives = 255/279 (91.40%), Query Frame = 0


Query: 26  PATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYN 85
           PA VFREYIGAEFN+VKFSDVPI  N+EEFHFLLSFAIDFD+S R FTNG F+VFWDS N
Sbjct: 33  PANVFREYIGAEFNNVKFSDVPIRSNIEEFHFLLSFAIDFDASSRSFTNGAFDVFWDSDN 92

Query: 86  LSPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNL 145
           L+PSAVA+IKKDHSNVRVGVSLGGD+V + GFV+FKPSSI SWVSNAE SLTK+INT+NL
Sbjct: 93  LNPSAVASIKKDHSNVRVGVSLGGDTVDNGGFVFFKPSSIHSWVSNAEHSLTKIINTYNL 152

Query: 146 DGIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGH 205
           DGIDIDYEHF+SDP+TFAECIGQLI+RLKKKG+ISFASIAPFDD EVQ+HYLALW+ YGH
Sbjct: 153 DGIDIDYEHFKSDPDTFAECIGQLIKRLKKKGIISFASIAPFDDDEVQSHYLALWRSYGH 212

Query: 206 LIDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDS-GGLLPKHGFFKAC 265
           LIDYVNFQFYAYDK TTV QFI+HFK Q S YE GR+LASLASD+S GGL+P++GFFKAC
Sbjct: 213 LIDYVNFQFYAYDKGTTVGQFIRHFKDQVSNYEGGRVLASLASDNSAGGLVPENGFFKAC 272

Query: 266 RRLRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           RRL+ +KLL GIFIWSADDS+A+GF YEK+AQ+LL +PN
Sbjct: 273 RRLKREKLLDGIFIWSADDSKAKGFGYEKEAQSLLATPN 311

[Close]

BLAST of Carg12349-RA vs. TrEMBL
Match: tr|M5VZB8|M5VZB8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G088400 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.8e-111
Identity = 198/275 (72.00%), Postives = 229/275 (83.27%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP 88
           +FREYIGAEFNDVKFSDVPINPNV EFHF+LSFAID+D S    TNG FNVFWDS NLSP
Sbjct: 34  LFREYIGAEFNDVKFSDVPINPNV-EFHFILSFAIDYDPSGSSPTNGKFNVFWDSDNLSP 93

Query: 89  SAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGI 148
           S V++IK  HSNV+V +SLGGDSV  SG  YFKPSSI SWVSNA  SLT +I  +NLDGI
Sbjct: 94  SQVSSIKNQHSNVKVALSLGGDSV-SSGSAYFKPSSIDSWVSNAVSSLTSIIQQYNLDGI 153

Query: 149 DIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLID 208
           DIDYEHF +DP+TF+ECIG+LI  LK  GVISFASIAPFDD +VQ+HYLALWK YGHLID
Sbjct: 154 DIDYEHFSADPDTFSECIGKLITTLKNNGVISFASIAPFDDDQVQSHYLALWKSYGHLID 213

Query: 209 YVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRLR 268
           YVNFQFYAYD+ TTV QFI +FK QSS Y  G++LAS  SD SGGL P++GFF AC RL+
Sbjct: 214 YVNFQFYAYDQGTTVSQFINYFKTQSSNYNGGKVLASFISDGSGGLSPENGFFTACHRLK 273

Query: 269 TDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           +++ LHGIF+WSADDS+  GFRYEK++QALL +P+
Sbjct: 274 SEQQLHGIFVWSADDSKKIGFRYEKQSQALLATPH 306

[Close]

BLAST of Carg12349-RA vs. TrEMBL
Match: tr|A0A2P6QI35|A0A2P6QI35_ROSCH (Putative chitinase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0062101 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.6e-110
Identity = 195/275 (70.91%), Postives = 233/275 (84.73%), Query Frame = 0


Query: 29  VFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNLSP 88
           +FREYIGAEFN+VKFSDVPINPNV EFHFLLSFAID+D+S    TNG FNVFWD+ NL  
Sbjct: 34  IFREYIGAEFNNVKFSDVPINPNV-EFHFLLSFAIDYDTSGSSPTNGNFNVFWDTDNLGA 93

Query: 89  SAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLDGI 148
           S V+AIK DHSNV+VG+SLGGDSV +SG  YF PSS+ SWVSNA  SLT +I  +NLDGI
Sbjct: 94  SQVSAIKNDHSNVKVGLSLGGDSV-NSGSCYFNPSSVDSWVSNAVSSLTSIIQQYNLDGI 153

Query: 149 DIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHLID 208
           DIDYEHF+SDPNTF+ECIG+LI+ LK KGVI FASIAPFDD +VQ+HYLALWK YG LID
Sbjct: 154 DIDYEHFKSDPNTFSECIGRLIKTLKDKGVIKFASIAPFDDDDVQSHYLALWKNYGQLID 213

Query: 209 YVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRRLR 268
           YVNFQFYAYD++TTV QFI +FK QSS Y  G++LAS ++D SGGL P++GFF AC RL+
Sbjct: 214 YVNFQFYAYDQSTTVSQFINYFKKQSSNYNGGKVLASFSTDGSGGLSPENGFFTACHRLK 273

Query: 269 TDKLLHGIFIWSADDSQARGFRYEKKAQALLQSPN 304
           +++ L+GIF+WSADDS+  GFRYEK++QALL +P+
Sbjct: 274 SEQNLNGIFVWSADDSKKNGFRYEKQSQALLATPH 306

[Close]

BLAST of Carg12349-RA vs. TrEMBL
Match: tr|A0A2P5EF55|A0A2P5EF55_9ROSA (2S globulin OS=Trema orientalis OX=63057 GN=TorRG33x02_200750 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 9.9e-110
Identity = 196/275 (71.27%), Postives = 228/275 (82.91%), Query Frame = 0


Query: 27  ATVFREYIGAEFNDVKFSDVPINPNVEEFHFLLSFAIDFDSSRRCFTNGVFNVFWDSYNL 86
           + +FREYIGAEFN+VKFSDVPINPNV +FHF+LSFAID+D+S    TNG FNVFWDS NL
Sbjct: 31  SNLFREYIGAEFNNVKFSDVPINPNV-DFHFILSFAIDYDASGTTPTNGKFNVFWDSENL 90

Query: 87  SPSAVAAIKKDHSNVRVGVSLGGDSVGDSGFVYFKPSSIASWVSNAERSLTKLINTFNLD 146
           SPS V++IK  H NV+V +SLGGDSVGD GF YFKP S+ SWVSNA  +LT++I  +NLD
Sbjct: 91  SPSQVSSIKSSHPNVKVALSLGGDSVGD-GFAYFKPFSVDSWVSNAVSTLTRIIKEYNLD 150

Query: 147 GIDIDYEHFESDPNTFAECIGQLIRRLKKKGVISFASIAPFDDAEVQTHYLALWKRYGHL 206
           GIDIDYEHF SDP TFAECIG+LI+ LK+  VISFASIAPFDD +VQ+HYLALWKRYG+L
Sbjct: 151 GIDIDYEHFSSDPQTFAECIGRLIKTLKRNKVISFASIAPFDDDQVQSHYLALWKRYGNL 210

Query: 207 IDYVNFQFYAYDKTTTVHQFIKHFKAQSSKYEDGRILASLASDDSGGLLPKHGFFKACRR 266
           IDYVNFQFYAYD+ TTV QFI +FK QSS Y  G++LAS  SD SGGL P +GFF AC R
Sbjct: 211 IDYVNFQFYAYDQGTTVSQFIDYFKTQSSNYHGGKVLASFISDGSGGLSPDNGFFDACSR 270

Query: 267 LRTDKLLHGIFIWSADDSQARGFRYEKKAQALLQS 302
           L++   LHGIFIWSADDS+  GFRYEK++QALL S
Sbjct: 271 LKSQGHLHGIFIWSADDSKKNGFRYEKRSQALLAS 303

The following BLAST results are available for this feature:

BLAST of Carg12349-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524573.1	8.6e-158	97.05	chitinase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022949055.1	1.1e-157	98.56	chitinase 2-like [Cucurbita moschata]	[more]
XP_004152902.1	4.0e-131	82.01	PREDICTED: chitinase 2-like [Cucumis sativus] >KGN65683.1 hypothetical protein C...	[more]
XP_008457518.1	3.8e-129	80.65	PREDICTED: chitinase 2-like [Cucumis melo]	[more]
XP_022156744.1	2.5e-128	81.59	chitinase 2-like [Momordica charantia]	[more]

BLAST of Carg12349-RA vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Carg12349-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9SLP4\|CHIT1_TULBA	5.1e-101	63.93	Chitinase 1 OS=Tulipa bakeri OX=110455 PE=1 SV=1	[more]
sp\|Q7M443\|CHIT2_TULBA	1.6e-99	64.13	Chitinase 2 OS=Tulipa bakeri OX=110455 PE=1 SV=1	[more]
sp\|P39657\|RUAP_SOYBN	6.8e-21	34.57	RuBisCO-associated protein OS=Glycine max OX=3847 PE=2 SV=1	[more]

BLAST of Carg12349-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LV90\|A0A0A0LV90_CUCSA	2.7e-131	82.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G496300 PE=3 SV=1	[more]
tr\|A0A1S3C5A3\|A0A1S3C5A3_CUCME	2.5e-129	80.65	chitinase 2-like OS=Cucumis melo OX=3656 GN=LOC103497192 PE=3 SV=1	[more]
tr\|M5VZB8\|M5VZB8_PRUPE	6.8e-111	72.00	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G088400 PE=3 SV=1	[more]
tr\|A0A2P6QI35\|A0A2P6QI35_ROSCH	2.6e-110	70.91	Putative chitinase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0062101 PE=3 SV=1	[more]
tr\|A0A2P5EF55\|A0A2P5EF55_9ROSA	9.9e-110	71.27	2S globulin OS=Trema orientalis OX=63057 GN=TorRG33x02_200750 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR017853	Glycoside_hydrolase_SF
IPR001579	Glyco_hydro_18_chit_AS
IPR001223	Glyco_hydro18_cat
IPR000677	2S_globulin

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12349	Carg12349	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12349-RA.exon.1	Carg12349-RA.exon.1	exon
Carg12349-RA.exon.2	Carg12349-RA.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12349-RA.CDS.1	Carg12349-RA.CDS.1	CDS
Carg12349-RA.CDS.2	Carg12349-RA.CDS.2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12349-RA	Carg12349-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000677	2S globulin	PRINTS	PR00551	2SGLOBULIN	coord: 27..40 score: 46.43 coord: 74..89 score: 27.6 coord: 91..109 score: 40.35 coord: 262..281 score: 37.08
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 89..224 e-value: 3.1E-14 score: 53.3
None	No IPR available	GENE3D	G3DSA:3.20.20.80		coord: 26..301 e-value: 6.0E-83 score: 280.5
None	No IPR available	PANTHER	PTHR11177:SF253	SUBFAMILY NOT NAMED	coord: 7..300
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 7..300
None	No IPR available	CDD	cd06544	GH18_narbonin	coord: 29..284 e-value: 1.55401E-120 score: 348.592
IPR001579	Glycoside hydrolase, chitinase active site	PROSITE	PS01095	CHITINASE_18	coord: 145..153
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	SSF51445	(Trans)glycosidases	coord: 28..299