Carg12004-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12004-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Urease accessory protein UreF
Location	Cucurbita_argyrosperma_scaffold_037 : 423872 .. 424558 (+)
Sequence length	687

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGACAAACAATTTCACTGGAGCCAGTGGCAACTGCTCGATTCTATCCTTCCTTCTGGTGGCTTTGCCCATTCGTTCGGTCTCGAGGCTGCAATTCAAGCTCGCATTGTCTCACAACCAGAAGATCTCAAAACGTTTGTGATCCATTTGTTAGACAACACTGGAAGTTTGCTTCTTCCTTTTGTGCATTCGACCACGCTATCACCTGATTTAGAAACTTGGGAGAATAATGACAGATTCTTGAATGCAATTTTGACAAATGAAGTTAGTCGAAAGGCGTCCGTTACGCAAGGATCGGCACTCATGAGAGTTGCAGCAATAGTATTTTCTGAAATCTCGTCTCTGAAAGCGATGAGAGAAACCACCACAGGGACCGGGGCTGTTTCCTTCCACCACGCTCCCATATTCGGGCTAATCTGCGGTCTACTAGGATGGGATAGCACGATGTCGCAGAGGGCGTATCTGTTTATTACCCTGAGAGACGTGATTTCTGCTGCAACAAGACTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAGCATCAGCTTGCGCTTGTTGCTGAAGACACATTGAAAAAATGGATGAACCGTCCTGTGGAAGAAGCTTGCCAAACGGTTCCTCTGTTAGAGACAGTACAGGGATGCCATACGTACCTGTTTTCTAGGCTGTTCTGTTCTTGA

mRNA sequence

ATGGATGACAAACAATTTCACTGGAGCCAGTGGCAACTGCTCGATTCTATCCTTCCTTCTGGTGGCTTTGCCCATTCGTTCGGTCTCGAGGCTGCAATTCAAGCTCGCATTGTCTCACAACCAGAAGATCTCAAAACGTTTGTGATCCATTTGTTAGACAACACTGGAAGTTTGCTTCTTCCTTTTGTGCATTCGACCACGCTATCACCTGATTTAGAAACTTGGGAGAATAATGACAGATTCTTGAATGCAATTTTGACAAATGAAGTTAGTCGAAAGGCGTCCGTTACGCAAGGATCGGCACTCATGAGAGTTGCAGCAATAGTATTTTCTGAAATCTCGTCTCTGAAAGCGATGAGAGAAACCACCACAGGGACCGGGGCTGTTTCCTTCCACCACGCTCCCATATTCGGGCTAATCTGCGGTCTACTAGGATGGGATAGCACGATGTCGCAGAGGGCGTATCTGTTTATTACCCTGAGAGACGTGATTTCTGCTGCAACAAGACTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAGCATCAGCTTGCGCTTGTTGCTGAAGACACATTGAAAAAATGGATGAACCGTCCTGTGGAAGAAGCTTGCCAAACGGTTCCTCTGTTAGAGACAGTACAGGGATGCCATACGTACCTGTTTTCTAGGCTGTTCTGTTCTTGA

Coding sequence (CDS)

ATGGATGACAAACAATTTCACTGGAGCCAGTGGCAACTGCTCGATTCTATCCTTCCTTCTGGTGGCTTTGCCCATTCGTTCGGTCTCGAGGCTGCAATTCAAGCTCGCATTGTCTCACAACCAGAAGATCTCAAAACGTTTGTGATCCATTTGTTAGACAACACTGGAAGTTTGCTTCTTCCTTTTGTGCATTCGACCACGCTATCACCTGATTTAGAAACTTGGGAGAATAATGACAGATTCTTGAATGCAATTTTGACAAATGAAGTTAGTCGAAAGGCGTCCGTTACGCAAGGATCGGCACTCATGAGAGTTGCAGCAATAGTATTTTCTGAAATCTCGTCTCTGAAAGCGATGAGAGAAACCACCACAGGGACCGGGGCTGTTTCCTTCCACCACGCTCCCATATTCGGGCTAATCTGCGGTCTACTAGGATGGGATAGCACGATGTCGCAGAGGGCGTATCTGTTTATTACCCTGAGAGACGTGATTTCTGCTGCAACAAGACTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAGCATCAGCTTGCGCTTGTTGCTGAAGACACATTGAAAAAATGGATGAACCGTCCTGTGGAAGAAGCTTGCCAAACGGTTCCTCTGTTAGAGACAGTACAGGGATGCCATACGTACCTGTTTTCTAGGCTGTTCTGTTCTTGA

Protein sequence

MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS

Homology

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BLAST of Carg12004-RA vs. NCBI nr
Match: XP_022946416.1 (urease accessory protein F [Cucurbita moschata])

HSP 1 Score: 453.4 bits (1165), Expect = 4.7e-124
Identity = 226/228 (99.12%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MDDKQFHWSQWQLLDSILP+GGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFVHSTTLSPDLETWE NDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

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BLAST of Carg12004-RA vs. NCBI nr
Match: XP_023545319.1 (urease accessory protein F [Cucurbita pepo subsp. pepo])

HSP 1 Score: 451.8 bits (1161), Expect = 1.4e-123
Identity = 225/228 (98.68%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MDDKQFHWSQWQLLDSILP+GGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL
Sbjct: 14  MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 73

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFVHSTTLSPDLETWE NDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR
Sbjct: 74  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 133

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           ET+TGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 134 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 193

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS
Sbjct: 194 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 241

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BLAST of Carg12004-RA vs. NCBI nr
Match: XP_022999571.1 (urease accessory protein F [Cucurbita maxima] >XP_022999572.1 urease accessory protein F [Cucurbita maxima])

HSP 1 Score: 448.7 bits (1153), Expect = 1.1e-122
Identity = 223/228 (97.81%), Postives = 226/228 (99.12%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MDDKQFHWSQWQLLDSILP+GGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFVHSTTLSPDLETWE NDRFLNAILTNEVSRKAS+TQGSALMRVAAIVFSEISSLKAMR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASITQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           ET+TGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 128 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           LQHQLALVAE TLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS
Sbjct: 188 LQHQLALVAEGTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

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BLAST of Carg12004-RA vs. NCBI nr
Match: XP_022153866.1 (urease accessory protein F [Momordica charantia] >XP_022153867.1 urease accessory protein F [Momordica charantia])

HSP 1 Score: 411.4 bits (1056), Expect = 2.0e-111
Identity = 201/228 (88.16%), Postives = 213/228 (93.42%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MD K   W+QWQLLDS+LP+GGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFVHS TLSPDLETW+ NDR L+A+LTNEV RKAS+TQGSALMRVAA+VFSEI SLK MR
Sbjct: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           LQHQLALVAED LKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 181 LQHQLALVAEDILKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 228

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BLAST of Carg12004-RA vs. NCBI nr
Match: XP_004144755.1 (PREDICTED: urease accessory protein F [Cucumis sativus] >XP_011648859.1 PREDICTED: urease accessory protein F [Cucumis sativus] >XP_011648860.1 PREDICTED: urease accessory protein F [Cucumis sativus] >KGN60994.1 hypothetical protein Csa_2G033980 [Cucumis sativus])

HSP 1 Score: 403.7 bits (1036), Expect = 4.2e-109
Identity = 199/228 (87.28%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MDDK  HWSQWQLLDSILP+GGFAHSFGLEAAIQA+IVS P+DLKTFVIHLLDNTGSL L
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFVHS T SPD ETW+ ND  L+A+LTNEVSRKASVTQGSALMRV+AIVFSEI SLKAMR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           LQHQLA VAED LK+WMNRPVEEACQTVPLLETVQGCH+ LFS++FCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

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BLAST of Carg12004-RA vs. TAIR10
Match: AT1G21840.1 (urease accessory protein F)

HSP 1 Score: 328.2 bits (840), Expect = 4.1e-90
Identity = 156/221 (70.59%), Postives = 186/221 (84.16%), Query Frame = 0


Query: 8   WSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPFVHSTT 67
           WSQWQLLDSILP+GGFAHSFGLEAAIQ R+VS PEDL+T +IH+LDNT SLLLPFV+S  
Sbjct: 20  WSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYSAL 79

Query: 68  LSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTG 127
            SPD+ETW   D  LNA LTN+VS KAS++QGSAL R+AA VF+E+ +LK +R+ + G+ 
Sbjct: 80  KSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLGSK 139

Query: 128 AVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAL 187
            V FHHAPIFGL+CGLLG DS  SQRAYLF+TLRDV+SAATRLN+VGP+GA+V+QH++A+
Sbjct: 140 NVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRIAI 199

Query: 188 VAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           V E  L+KWMNR   EACQT PLL+ VQGCH YLFSRLFCS
Sbjct: 200 VTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

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BLAST of Carg12004-RA vs. Swiss-Prot
Match: sp|Q9XHZ3|UREF_ARATH (Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 7.4e-89
Identity = 156/221 (70.59%), Postives = 186/221 (84.16%), Query Frame = 0


Query: 8   WSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPFVHSTT 67
           WSQWQLLDSILP+GGFAHSFGLEAAIQ R+VS PEDL+T +IH+LDNT SLLLPFV+S  
Sbjct: 20  WSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYSAL 79

Query: 68  LSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTG 127
            SPD+ETW   D  LNA LTN+VS KAS++QGSAL R+AA VF+E+ +LK +R+ + G+ 
Sbjct: 80  KSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLGSK 139

Query: 128 AVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAL 187
            V FHHAPIFGL+CGLLG DS  SQRAYLF+TLRDV+SAATRLN+VGP+GA+V+QH++A+
Sbjct: 140 NVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRIAI 199

Query: 188 VAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           V E  L+KWMNR   EACQT PLL+ VQGCH YLFSRLFCS
Sbjct: 200 VTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

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BLAST of Carg12004-RA vs. Swiss-Prot
Match: sp|E0ZS46|UREF_ORYSI (Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 2.7e-83
Identity = 149/221 (67.42%), Postives = 187/221 (84.62%), Query Frame = 0


Query: 8   WSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPFVHSTT 67
           WSQWQLLDSILP+GGFAHS+GLEAA+Q+R+V+ PE+L++FV+ +L+NTGSLLLPFV    
Sbjct: 41  WSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPFVCCAN 100

Query: 68  LSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTG 127
            SPD  TW   D+ L A+LTNEVSRKAS++QGSAL+RVAA VF+EI SL+ +R+T  G+ 
Sbjct: 101 KSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQTFLGSK 160

Query: 128 AVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAL 187
            VSFHHAPIFGLICGL+G+DS  +QRAY+F+T+RDVISAATRLNL+GPL A+VLQHQ+A 
Sbjct: 161 IVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQHQVAE 220

Query: 188 VAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
            AE  ++KW +R VEEA QT PLL+ +QGCH Y+FSRLFC+
Sbjct: 221 DAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

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BLAST of Carg12004-RA vs. Swiss-Prot
Match: sp|Q0E3L5|UREF_ORYSJ (Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2 SV=2)

HSP 1 Score: 309.7 bits (792), Expect = 2.7e-83
Identity = 149/221 (67.42%), Postives = 187/221 (84.62%), Query Frame = 0


Query: 8   WSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPFVHSTT 67
           WSQWQLLDSILP+GGFAHS+GLEAA+Q+R+V+ PE+L++FV+ +L+NTGSLLLPFV    
Sbjct: 41  WSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPFVCCAN 100

Query: 68  LSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTG 127
            SPD  TW   D+ L A+LTNEVSRKAS++QGSAL+RVAA VF+EI SL+ +R+T  G+ 
Sbjct: 101 KSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQTFLGSK 160

Query: 128 AVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAL 187
            VSFHHAPIFGLICGL+G+DS  +QRAY+F+T+RDVISAATRLNL+GPL A+VLQHQ+A 
Sbjct: 161 IVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQHQVAE 220

Query: 188 VAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
            AE  ++KW +R VEEA QT PLL+ +QGCH Y+FSRLFC+
Sbjct: 221 DAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

[Close]

BLAST of Carg12004-RA vs. Swiss-Prot
Match: sp|B7K911|UREF_CYAP7 (Urease accessory protein UreF OS=Cyanothece sp. (strain PCC 7424) OX=65393 GN=ureF PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.6e-14
Identity = 67/221 (30.32%), Postives = 100/221 (45.25%), Query Frame = 0


Query: 12  QLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDN----TGSLLLPFVHSTT 71
           QL DS  PSG F  S GLEA  Q   +   +DL+ F+  LL N    T  + L   +  +
Sbjct: 14  QLSDSFFPSGSFTLSHGLEALAQGEQIHSIKDLEIFLQILLHNKLGTTDLVALIHAYRGS 73

Query: 72  LSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTG 131
              DL+     D  L A    E +R+     G AL+ VA   + +       +ET  GT 
Sbjct: 74  KREDLDAVREADHQLFAQTLIEKNREMGRKSGRALLMVARETWQDTQLETLEKETAKGT- 133

Query: 132 AVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAL 191
            ++  H  IF ++  + G   T +  A+L   +  ++ AA RLN++G L A  +  +LA 
Sbjct: 134 -INGLHPIIFAVVGRVAGLSETDTGLAFLHSFITGLLGAAIRLNIIGHLQAQKILLKLAP 193

Query: 192 VAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
             E T +K     + E     PL++  Q  H  L  RLF +
Sbjct: 194 DLETTYQKATEINLNEMFSCTPLIDIAQMNHQNLDYRLFAN 232

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BLAST of Carg12004-RA vs. Swiss-Prot
Match: sp|Q117Z4|UREF_TRIEI (Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=ureF PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.6e-11
Identity = 62/221 (28.05%), Postives = 101/221 (45.70%), Query Frame = 0


Query: 12  QLLDSILPSGGFAHSFGLEAAIQ-ARIVSQPEDLKTFVIHLLDNTG-SLLLPFVHS--TT 71
           QL DS  P+G F  S GLE  +Q  +I SQPE L    I L +  G S ++  +HS    
Sbjct: 14  QLSDSFFPTGSFTFSHGLETLVQTGKIQSQPEILYFLQILLRNKVGVSDVVALIHSYRGC 73

Query: 72  LSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRETTTGTG 131
            + D+E     DR L        +R+     G AL+ VA+  + +   LK +      +G
Sbjct: 74  KNGDIEAVRVADRMLFVQTAIAKNRETQRQSGRALLMVASSTWQD-ERLKTLNIDAV-SG 133

Query: 132 AVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQHQLAL 191
            +   H  IFG +  ++G D   +  A+L   + +++ AA RL ++G + A  +  QL  
Sbjct: 134 NIHCLHPVIFGAVTSVVGLDERNAVLAFLHGLVTNILGAAIRLGILGHIQAQQILLQLVP 193

Query: 192 VAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
             E      ++  +E+     P ++  Q  H  L  +LF +
Sbjct: 194 DIEAVWSTAVSMNLEQMWSCTPFIDIAQMQHPKLAHKLFAN 232

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BLAST of Carg12004-RA vs. TrEMBL
Match: tr|A0A0A0LJ08|A0A0A0LJ08_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.8e-109
Identity = 199/228 (87.28%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MDDK  HWSQWQLLDSILP+GGFAHSFGLEAAIQA+IVS P+DLKTFVIHLLDNTGSL L
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFVHS T SPD ETW+ ND  L+A+LTNEVSRKASVTQGSALMRV+AIVFSEI SLKAMR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTGAVSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           LQHQLA VAED LK+WMNRPVEEACQTVPLLETVQGCH+ LFS++FCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

[Close]

BLAST of Carg12004-RA vs. TrEMBL
Match: tr|A0A1S3BWP6|A0A1S3BWP6_CUCME (urease accessory protein F OS=Cucumis melo OX=3656 GN=LOC103494267 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.6e-101
Identity = 189/213 (88.73%), Postives = 198/213 (92.96%), Query Frame = 0


Query: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60
           MDDK+ HWSQWQLLDSILP+GGFAHSFGLEAAIQA+IVS PEDLKTFVIHLLDNTGSL L
Sbjct: 1   MDDKRCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPEDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120
           PFV+S TLSPD ETW+ NDR L+AILTNEVSRKASVTQGSALMRV+AIVFSEISSLKAMR
Sbjct: 61  PFVYSATLSPDFETWKKNDRLLDAILTNEVSRKASVTQGSALMRVSAIVFSEISSLKAMR 120

Query: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180
           E   GTG VSFHHAPIFGLICGLLGWD TMSQRAYLFITLRDVISAATRLNLVGPLGAAV
Sbjct: 121 EHLYGTGTVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLET 214
           LQHQLA VAE+ LK+WMNRPVEEACQ VPLLET
Sbjct: 181 LQHQLAQVAENVLKRWMNRPVEEACQAVPLLET 213

[Close]

BLAST of Carg12004-RA vs. TrEMBL
Match: tr|A0A2I4FN91|A0A2I4FN91_9ROSI (urease accessory protein F isoform X1 OS=Juglans regia OX=51240 GN=LOC109000628 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.7e-96
Identity = 176/226 (77.88%), Postives = 198/226 (87.61%), Query Frame = 0


Query: 3   DKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPF 62
           D    WSQWQL+DSILP+GGFAHSFGLEAAIQARI+S PEDL+TFVIH+L+NTGSLLLPF
Sbjct: 87  DSHLQWSQWQLIDSILPTGGFAHSFGLEAAIQARIISGPEDLQTFVIHVLENTGSLLLPF 146

Query: 63  VHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRET 122
           V S T+SPDLETW+  DR L+A+LTNEV RKAS+ QGSALMRVAA VFSE  SLK+MR+T
Sbjct: 147 VFSATISPDLETWQKLDRTLDAMLTNEVGRKASIAQGSALMRVAAAVFSETPSLKSMRDT 206

Query: 123 TTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
           + G+G VSFHHAPIFGL CGLLG DS  SQRAYLFIT+RDVISAATRLNLVGP+GAAVLQ
Sbjct: 207 SVGSGVVSFHHAPIFGLTCGLLGLDSRTSQRAYLFITMRDVISAATRLNLVGPMGAAVLQ 266

Query: 183 HQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           HQ+ ++AE  L KWM+R VEEACQT PLL+TVQGCH YLFSRLFCS
Sbjct: 267 HQIVVLAEAILTKWMDRTVEEACQTSPLLDTVQGCHAYLFSRLFCS 312

[Close]

BLAST of Carg12004-RA vs. TrEMBL
Match: tr|A0A0D2W4U1|A0A0D2W4U1_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_013G107000 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 9.7e-94
Identity = 168/226 (74.34%), Postives = 199/226 (88.05%), Query Frame = 0


Query: 3   DKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPF 62
           D    WSQWQLLDSILP+GGFAHSFGLEAAIQAR+VS P+DL+TFVIHLL+NTGSLLLPF
Sbjct: 58  DPSLQWSQWQLLDSILPTGGFAHSFGLEAAIQARLVSGPDDLQTFVIHLLENTGSLLLPF 117

Query: 63  VHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRET 122
           V+S T++P L+ W+  DR L+A L+NEV RKASV+QGS+LMRVAA VF+E+ SLK+MR+ 
Sbjct: 118 VYSATMNPTLDNWQKLDRILDATLSNEVGRKASVSQGSSLMRVAASVFTELPSLKSMRDV 177

Query: 123 TTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
             G+G VSFHHAP+FG+ICGLLG DS  SQRAY+F+T+RDVISAATRLNLVGPLGAAVLQ
Sbjct: 178 CIGSGVVSFHHAPVFGMICGLLGMDSGTSQRAYIFMTMRDVISAATRLNLVGPLGAAVLQ 237

Query: 183 HQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           H++A VAE  +KKWM+R VE+ACQT PLL+T+QGCHTYLFSRLFCS
Sbjct: 238 HRVAYVAESMVKKWMDRSVEDACQTAPLLDTIQGCHTYLFSRLFCS 283

[Close]

BLAST of Carg12004-RA vs. TrEMBL
Match: tr|A0A1U8NKR7|A0A1U8NKR7_GOSHI (urease accessory protein F-like OS=Gossypium hirsutum OX=3635 GN=LOC107949525 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.3e-93
Identity = 168/226 (74.34%), Postives = 199/226 (88.05%), Query Frame = 0


Query: 3   DKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLLPF 62
           D    WSQWQLLDSILP+GGFAHSFGLEAAIQAR+VS P+DL+TFVIHLL+NTGSLLLPF
Sbjct: 58  DPSLKWSQWQLLDSILPTGGFAHSFGLEAAIQARLVSGPDDLQTFVIHLLENTGSLLLPF 117

Query: 63  VHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMRET 122
           V+S T++P L+ W+  DR L+A L+NEV RKASV+QGS+LMRVAA VF+E+ SLK+MR+ 
Sbjct: 118 VYSATMNPTLDNWQKLDRILDATLSNEVGRKASVSQGSSLMRVAASVFTELPSLKSMRDV 177

Query: 123 TTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAVLQ 182
             G+G VSFHHAP+FG+ICGLLG DS  SQRAY+F+T+RDVISAATRLNLVGPLGAAVLQ
Sbjct: 178 CIGSGVVSFHHAPVFGMICGLLGMDSGTSQRAYIFMTMRDVISAATRLNLVGPLGAAVLQ 237

Query: 183 HQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 229
           H++A VAE  +KKWM+R VE+ACQT PLL+T+QGCHTYLFSRLFCS
Sbjct: 238 HRVAYVAESMVKKWMDRSVEDACQTAPLLDTIQGCHTYLFSRLFCS 283

The following BLAST results are available for this feature:

BLAST of Carg12004-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946416.1	4.7e-124	99.12	urease accessory protein F [Cucurbita moschata]	[more]
XP_023545319.1	1.4e-123	98.68	urease accessory protein F [Cucurbita pepo subsp. pepo]	[more]
XP_022999571.1	1.1e-122	97.81	urease accessory protein F [Cucurbita maxima] >XP_022999572.1 urease accessory p...	[more]
XP_022153866.1	2.0e-111	88.16	urease accessory protein F [Momordica charantia] >XP_022153867.1 urease accessor...	[more]
XP_004144755.1	4.2e-109	87.28	PREDICTED: urease accessory protein F [Cucumis sativus] >XP_011648859.1 PREDICTE...	[more]

BLAST of Carg12004-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G21840.1	4.1e-90	70.59	urease accessory protein F	[more]

BLAST of Carg12004-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9XHZ3\|UREF_ARATH	7.4e-89	70.59	Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1	[more]
sp\|E0ZS46\|UREF_ORYSI	2.7e-83	67.42	Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 S...	[more]
sp\|Q0E3L5\|UREF_ORYSJ	2.7e-83	67.42	Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2...	[more]
sp\|B7K911\|UREF_CYAP7	4.6e-14	30.32	Urease accessory protein UreF OS=Cyanothece sp. (strain PCC 7424) OX=65393 GN=ur...	[more]
sp\|Q117Z4\|UREF_TRIEI	1.6e-11	28.05	Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203...	[more]

BLAST of Carg12004-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LJ08\|A0A0A0LJ08_CUCSA	2.8e-109	87.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1	[more]
tr\|A0A1S3BWP6\|A0A1S3BWP6_CUCME	1.6e-101	88.73	urease accessory protein F OS=Cucumis melo OX=3656 GN=LOC103494267 PE=4 SV=1	[more]
tr\|A0A2I4FN91\|A0A2I4FN91_9ROSI	2.7e-96	77.88	urease accessory protein F isoform X1 OS=Juglans regia OX=51240 GN=LOC109000628 ...	[more]
tr\|A0A0D2W4U1\|A0A0D2W4U1_GOSRA	9.7e-94	74.34	Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_013G107000 PE=4 ...	[more]
tr\|A0A1U8NKR7\|A0A1U8NKR7_GOSHI	1.3e-93	74.34	urease accessory protein F-like OS=Gossypium hirsutum OX=3635 GN=LOC107949525 PE...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016151	nickel cation binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006807	nitrogen compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002639	UreF
IPR038277	UreF_sf

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016151	nickel cation binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12004	Carg12004	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12004-RA.exon.1	Carg12004-RA.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12004-RA.CDS.1	Carg12004-RA.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12004-RA	Carg12004-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038277	UreF domain superfamily	GENE3D	G3DSA:1.10.4190.10		coord: 3..207 e-value: 2.0E-30 score: 108.1
IPR002639	Urease accessory protein UreF	PFAM	PF01730	UreF	coord: 38..185 e-value: 2.2E-17 score: 63.8
IPR002639	Urease accessory protein UreF	PIRSF	PIRSF009467	Urease_acces_UreF	coord: 3..228 e-value: 4.6E-45 score: 152.1
None	No IPR available	PANTHER	PTHR33620:SF1	UREASE ACCESSORY PROTEIN F	coord: 1..228
None	No IPR available	PANTHER	PTHR33620	FAMILY NOT NAMED	coord: 1..228