Carg09309-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg09309-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	thermospermine synthase ACAULIS5
Location	Cucurbita_argyrosperma_scaffold_018 : 512072 .. 512559 (-)
Sequence length	393

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGGTATTTGATTTCGCTAATTAACTTCCAAATCTCTTCGTATTTCTCCGTTCTAATTCCATCGCCGATTCATTTGTAAATCCGTTTCTTCTCTTCAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

mRNA sequence

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Coding sequence (CDS)

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Protein sequence

MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES

Homology

[Close]

BLAST of Carg09309-RA vs. NCBI nr
Match: XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 182.2 bits (461), Expect = 9.2e-43
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309-RA vs. NCBI nr
Match: XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])

HSP 1 Score: 181.0 bits (458), Expect = 2.1e-42
Identity = 86/87 (98.85%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309-RA vs. NCBI nr
Match: XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 180.3 bits (456), Expect = 3.5e-42
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309-RA vs. NCBI nr
Match: XP_008445532.2 (PREDICTED: thermospermine synthase ACAULIS5 [Cucumis melo])

HSP 1 Score: 157.1 bits (396), Expect = 3.2e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

[Close]

BLAST of Carg09309-RA vs. NCBI nr
Match: XP_022131411.1 (thermospermine synthase ACAULIS5 [Momordica charantia])

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-30
Identity = 71/87 (81.61%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1  MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTK FGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKRFGK 80

[Close]

BLAST of Carg09309-RA vs. TAIR10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 81.6 bits (200), Expect = 3.1e-16
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

[Close]

BLAST of Carg09309-RA vs. Swiss-Prot
Match: sp|Q9S7X6|ACL5_ARATH (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 5.5e-15
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

[Close]

BLAST of Carg09309-RA vs. TrEMBL
Match: tr|A0A1S3BCH1|A0A1S3BCH1_CUCME (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

[Close]

BLAST of Carg09309-RA vs. TrEMBL
Match: tr|A0A0A0KIK6|A0A0A0KIK6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.8e-24
Identity = 63/88 (71.59%), Postives = 68/88 (77.27%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHT               NY NGD SSHESVIN V+G+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHT---------------NYTNGDNSSHESVINNVSGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 73

[Close]

BLAST of Carg09309-RA vs. TrEMBL
Match: tr|A0A2I4EZY8|A0A2I4EZY8_9ROSI (thermospermine synthase ACAULIS5 OS=Juglans regia OX=51240 GN=LOC108994283 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-15
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MG AV   HTNGF+K          N +N     +++IN ++ NDC W+EE+ID +LKWS
Sbjct: 1  MGEAVEFFHTNGFTK----------NCVN-----QTLINDIHNNDC-WYEELIDVDLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGKV 89
          FALN +L KGTSE+QDIALLDTK FGKV
Sbjct: 61 FALNRILYKGTSEYQDIALLDTKRFGKV 72

[Close]

BLAST of Carg09309-RA vs. TrEMBL
Match: tr|V4UPQ2|V4UPQ2_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10008847mg PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-13
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 41 VNGN--DCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          +NGN  DC WFEE+ID++LKWSFALN VL KGTSEFQDIALLDTK FGKV
Sbjct: 25 INGNLQDCCWFEEVIDDDLKWSFALNRVLHKGTSEFQDIALLDTKRFGKV 74

[Close]

BLAST of Carg09309-RA vs. TrEMBL
Match: tr|A0A067GE17|A0A067GE17_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g019550mg PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-13
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 41 VNGN--DCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          +NGN  DC WFEE+ID++LKWSFALN VL KGTSEFQDIALLDTK FGKV
Sbjct: 25 INGNLQDCCWFEEVIDDDLKWSFALNRVLHKGTSEFQDIALLDTKRFGKV 74

The following BLAST results are available for this feature:

BLAST of Carg09309-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023546524.1	9.2e-43	100.00	thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022961616.1	2.1e-42	98.85	thermospermine synthase ACAULIS5 [Cucurbita moschata]	[more]
XP_022997196.1	3.5e-42	98.85	thermospermine synthase ACAULIS5-like [Cucurbita maxima]	[more]
XP_008445532.2	3.2e-35	87.50	PREDICTED: thermospermine synthase ACAULIS5 [Cucumis melo]	[more]
XP_022131411.1	2.4e-30	81.61	thermospermine synthase ACAULIS5 [Momordica charantia]	[more]

BLAST of Carg09309-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19530.1	3.1e-16	76.60	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

BLAST of Carg09309-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9S7X6\|ACL5_ARATH	5.5e-15	76.60	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]

BLAST of Carg09309-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BCH1\|A0A1S3BCH1_CUCME	2.1e-35	87.50	thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV...	[more]
tr\|A0A0A0KIK6\|A0A0A0KIK6_CUCSA	2.8e-24	71.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1	[more]
tr\|A0A2I4EZY8\|A0A2I4EZY8_9ROSI	2.4e-15	55.68	thermospermine synthase ACAULIS5 OS=Juglans regia OX=51240 GN=LOC108994283 PE=3 ...	[more]
tr\|V4UPQ2\|V4UPQ2_9ROSI	1.0e-13	80.00	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10008847mg PE=3 ...	[more]
tr\|A0A067GE17\|A0A067GE17_CITSI	1.0e-13	80.00	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g019550mg PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR030374	PABS
IPR037163	Spermidine_synt_N_sf
IPR035246	Spermidine_synt_N

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg09309	Carg09309	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09309-RA.CDS.2	Carg09309-RA.CDS.2	CDS
Carg09309-RA.CDS.1	Carg09309-RA.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09309-RA.five_prime_UTR.1	Carg09309-RA.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09309-RA.exon.2	Carg09309-RA.exon.2	exon
Carg09309-RA.exon.1	Carg09309-RA.exon.1	exon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg09309-RA	Carg09309-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 47..88 e-value: 2.0E-8 score: 33.9
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	G3DSA:2.30.140.10		coord: 36..90 e-value: 2.9E-10 score: 42.4
None	No IPR available	PANTHER	PTHR43317:SF2	SUBFAMILY NOT NAMED	coord: 24..89
None	No IPR available	PANTHER	PTHR43317	FAMILY NOT NAMED	coord: 24..89
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 46..105 score: 13.227