Carg09309 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg09309
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	thermospermine synthase ACAULIS5
Location	Cucurbita_argyrosperma_scaffold_018 : 512072 .. 512559 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGGTATTTGATTTCGCTAATTAACTTCCAAATCTCTTCGTATTTCTCCGTTCTAATTCCATCGCCGATTCATTTGTAAATCCGTTTCTTCTCTTCAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

mRNA sequence

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Coding sequence (CDS)

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Protein sequence

MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES

Homology

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 182.2 bits (461), Expect = 9.2e-43
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])

HSP 1 Score: 181.0 bits (458), Expect = 2.1e-42
Identity = 86/87 (98.85%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 180.3 bits (456), Expect = 3.5e-42
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_008445532.2 (PREDICTED: thermospermine synthase ACAULIS5 [Cucumis melo])

HSP 1 Score: 157.1 bits (396), Expect = 3.2e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

[Close]

BLAST of Carg09309 vs. NCBI nr
Match: XP_022131411.1 (thermospermine synthase ACAULIS5 [Momordica charantia])

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-30
Identity = 71/87 (81.61%), Postives = 79/87 (90.80%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1  MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTK FGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKRFGK 80

[Close]

BLAST of Carg09309 vs. TAIR10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 81.6 bits (200), Expect = 3.1e-16
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

[Close]

BLAST of Carg09309 vs. Swiss-Prot
Match: sp|Q9S7X6|ACL5_ARATH (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 5.5e-15
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0


Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

[Close]

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A1S3BCH1|A0A1S3BCH1_CUCME (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

[Close]

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A0A0KIK6|A0A0A0KIK6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.8e-24
Identity = 63/88 (71.59%), Postives = 68/88 (77.27%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHT               NY NGD SSHESVIN V+G+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHT---------------NYTNGDNSSHESVINNVSGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 73

[Close]

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A2I4EZY8|A0A2I4EZY8_9ROSI (thermospermine synthase ACAULIS5 OS=Juglans regia OX=51240 GN=LOC108994283 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-15
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MG AV   HTNGF+K          N +N     +++IN ++ NDC W+EE+ID +LKWS
Sbjct: 1  MGEAVEFFHTNGFTK----------NCVN-----QTLINDIHNNDC-WYEELIDVDLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGKV 89
          FALN +L KGTSE+QDIALLDTK FGKV
Sbjct: 61 FALNRILYKGTSEYQDIALLDTKRFGKV 72

[Close]

BLAST of Carg09309 vs. TrEMBL
Match: tr|V4UPQ2|V4UPQ2_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10008847mg PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-13
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 41 VNGN--DCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          +NGN  DC WFEE+ID++LKWSFALN VL KGTSEFQDIALLDTK FGKV
Sbjct: 25 INGNLQDCCWFEEVIDDDLKWSFALNRVLHKGTSEFQDIALLDTKRFGKV 74

[Close]

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A067GE17|A0A067GE17_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g019550mg PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-13
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 41 VNGN--DCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          +NGN  DC WFEE+ID++LKWSFALN VL KGTSEFQDIALLDTK FGKV
Sbjct: 25 INGNLQDCCWFEEVIDDDLKWSFALNRVLHKGTSEFQDIALLDTKRFGKV 74

The following BLAST results are available for this feature:

BLAST of Carg09309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023546524.1	9.2e-43	100.00	thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022961616.1	2.1e-42	98.85	thermospermine synthase ACAULIS5 [Cucurbita moschata]	[more]
XP_022997196.1	3.5e-42	98.85	thermospermine synthase ACAULIS5-like [Cucurbita maxima]	[more]
XP_008445532.2	3.2e-35	87.50	PREDICTED: thermospermine synthase ACAULIS5 [Cucumis melo]	[more]
XP_022131411.1	2.4e-30	81.61	thermospermine synthase ACAULIS5 [Momordica charantia]	[more]

BLAST of Carg09309 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19530.1	3.1e-16	76.60	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

BLAST of Carg09309 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9S7X6\|ACL5_ARATH	5.5e-15	76.60	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]

BLAST of Carg09309 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BCH1\|A0A1S3BCH1_CUCME	2.1e-35	87.50	thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV...	[more]
tr\|A0A0A0KIK6\|A0A0A0KIK6_CUCSA	2.8e-24	71.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1	[more]
tr\|A0A2I4EZY8\|A0A2I4EZY8_9ROSI	2.4e-15	55.68	thermospermine synthase ACAULIS5 OS=Juglans regia OX=51240 GN=LOC108994283 PE=3 ...	[more]
tr\|V4UPQ2\|V4UPQ2_9ROSI	1.0e-13	80.00	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10008847mg PE=3 ...	[more]
tr\|A0A067GE17\|A0A067GE17_CITSI	1.0e-13	80.00	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g019550mg PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR030374	PABS
IPR037163	Spermidine_synt_N_sf
IPR035246	Spermidine_synt_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09309-RA	Carg09309-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 47..88 e-value: 2.0E-8 score: 33.9
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	G3DSA:2.30.140.10		coord: 36..90 e-value: 2.9E-10 score: 42.4
None	No IPR available	PANTHER	PTHR43317:SF2	SUBFAMILY NOT NAMED	coord: 24..89
None	No IPR available	PANTHER	PTHR43317	FAMILY NOT NAMED	coord: 24..89
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 46..105 score: 13.227

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg09309	ClCG08G009870	Watermelon (Charleston Gray)	carwcgB0138

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg09309	Cucumber (Gy14) v2	carcgybB0156
Carg09309	Cucumber (Gy14) v1	carcgyB0168
Carg09309	Cucurbita maxima (Rimu)	carcmaB0189
Carg09309	Cucurbita moschata (Rifu)	carcmoB0196
Carg09309	Cucurbita pepo (Zucchini)	carcpeB0199
Carg09309	Wild cucumber (PI 183967)	carcpiB0163
Carg09309	Cucumber (Chinese Long) v2	carcuB0158
Carg09309	Cucumber (Chinese Long) v3	carcucB0166
Carg09309	Bottle gourd (USVL1VR-Ls)	carlsiB129
Carg09309	Melon (DHL92) v3.5.1	carmeB0142
Carg09309	Melon (DHL92) v3.6.1	carmedB0146
Carg09309	Watermelon (97103) v1	carwmB0134
Carg09309	Watermelon (97103) v2	carwmbB0150
Carg09309	Wax gourd	carwgoB0174
Carg09309	Wax gourd	carwgoB0177
Carg09309	Silver-seed gourd	carcarB168