Carg09309 (gene) Silver-seed gourd

NameCarg09309
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionthermospermine synthase ACAULIS5
LocationCucurbita_argyrosperma_scaffold_018 : 512072 .. 512559 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGGTATTTGATTTCGCTAATTAACTTCCAAATCTCTTCGTATTTCTCCGTTCTAATTCCATCGCCGATTCATTTGTAAATCCGTTTCTTCTCTTCAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

mRNA sequence

CAAAACTTCCGCCTCCCTGTTTCATAGACTCTCTCATTATATTCCCATTTTCCCCCTTTCTTGCTCCGTAAGGCGATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Coding sequence (CDS)

ATGGGTCCAGCAGTTCTGAGTTCACACACCAATGGCTTCTCGAAATCAGTAGATCGAAACTTCGAAGAGAAAAGCAATTACATCAATGGAGATTCTTCTCATGAATCGGTAATCAATACTGTTAATGGCAACGATTGCTATTGGTTCGAGGAAATCATCGACGAAAATCTCAAATGGTCTTTTGCTCTGAATAGCGTTCTGCAGAAAGGAACCAGCGAGTTTCAGGATATTGCGCTATTAGATACCAAGTGTTTCGGAAAAGTGCGTTTATCAAGTTTCGATTTCCGTTTCTGTTCGGCGATTTTCTGCGAGTCGTGA

Protein sequence

MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKVRLSSFDFRFCSAIFCES
BLAST of Carg09309 vs. NCBI nr
Match: XP_023546524.1 (thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 182.2 bits (461), Expect = 9.2e-43
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

BLAST of Carg09309 vs. NCBI nr
Match: XP_022961616.1 (thermospermine synthase ACAULIS5 [Cucurbita moschata])

HSP 1 Score: 181.0 bits (458), Expect = 2.1e-42
Identity = 86/87 (98.85%), Postives = 87/87 (100.00%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEK+NYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKNNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

BLAST of Carg09309 vs. NCBI nr
Match: XP_022997196.1 (thermospermine synthase ACAULIS5-like [Cucurbita maxima])

HSP 1 Score: 180.3 bits (456), Expect = 3.5e-42
Identity = 86/87 (98.85%), Postives = 86/87 (98.85%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVIN VNGNDCYWFEEIIDENLKWS
Sbjct: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINNVNGNDCYWFEEIIDENLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTKCFGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 87

BLAST of Carg09309 vs. NCBI nr
Match: XP_008445532.2 (PREDICTED: thermospermine synthase ACAULIS5 [Cucumis melo])

HSP 1 Score: 157.1 bits (396), Expect = 3.2e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

BLAST of Carg09309 vs. NCBI nr
Match: XP_022131411.1 (thermospermine synthase ACAULIS5 [Momordica charantia])

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-30
Identity = 71/87 (81.61%), Postives = 79/87 (90.80%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MGPAVLSSHTNGFSK     F++KSNYING+SSHE+VI+  NG+DC+WFEEIID+NLKWS
Sbjct: 1  MGPAVLSSHTNGFSK-----FDDKSNYINGNSSHEAVIS--NGSDCHWFEEIIDDNLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGK 88
          FALNSVLQKGTSEFQDIALLDTK FGK
Sbjct: 61 FALNSVLQKGTSEFQDIALLDTKRFGK 80

BLAST of Carg09309 vs. TAIR10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 81.6 bits (200), Expect = 3.1e-16
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0

Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

BLAST of Carg09309 vs. Swiss-Prot
Match: sp|Q9S7X6|ACL5_ARATH (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 5.5e-15
Identity = 36/47 (76.60%), Postives = 42/47 (89.36%), Query Frame = 0

Query: 42 NGNDCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          N  DC+W+EE ID++LKWSFALNSVL +GTSE+QDIALLDTK FGKV
Sbjct: 29 NQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKV 75

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A1S3BCH1|A0A1S3BCH1_CUCME (thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.1e-35
Identity = 77/88 (87.50%), Postives = 83/88 (94.32%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHTNGFS+SVDRNFEEK+NYIN D SSHESVIN VNG+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHTNGFSQSVDRNFEEKTNYINDDNSSHESVINNVNGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 88

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A0A0KIK6|A0A0A0KIK6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.8e-24
Identity = 63/88 (71.59%), Postives = 68/88 (77.27%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGD-SSHESVINTVNGNDCYWFEEIIDENLKW 60
          MGPAVLSSHT               NY NGD SSHESVIN V+G+DC+W+EEIIDENLKW
Sbjct: 1  MGPAVLSSHT---------------NYTNGDNSSHESVINNVSGDDCHWYEEIIDENLKW 60

Query: 61 SFALNSVLQKGTSEFQDIALLDTKCFGK 88
          SFALN VLQ+GTSEFQDIALLDTK FGK
Sbjct: 61 SFALNRVLQQGTSEFQDIALLDTKRFGK 73

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A2I4EZY8|A0A2I4EZY8_9ROSI (thermospermine synthase ACAULIS5 OS=Juglans regia OX=51240 GN=LOC108994283 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-15
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 0

Query: 1  MGPAVLSSHTNGFSKSVDRNFEEKSNYINGDSSHESVINTVNGNDCYWFEEIIDENLKWS 60
          MG AV   HTNGF+K          N +N     +++IN ++ NDC W+EE+ID +LKWS
Sbjct: 1  MGEAVEFFHTNGFTK----------NCVN-----QTLINDIHNNDC-WYEELIDVDLKWS 60

Query: 61 FALNSVLQKGTSEFQDIALLDTKCFGKV 89
          FALN +L KGTSE+QDIALLDTK FGKV
Sbjct: 61 FALNRILYKGTSEYQDIALLDTKRFGKV 72

BLAST of Carg09309 vs. TrEMBL
Match: tr|V4UPQ2|V4UPQ2_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10008847mg PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-13
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = 0

Query: 41 VNGN--DCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          +NGN  DC WFEE+ID++LKWSFALN VL KGTSEFQDIALLDTK FGKV
Sbjct: 25 INGNLQDCCWFEEVIDDDLKWSFALNRVLHKGTSEFQDIALLDTKRFGKV 74

BLAST of Carg09309 vs. TrEMBL
Match: tr|A0A067GE17|A0A067GE17_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g019550mg PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-13
Identity = 40/50 (80.00%), Postives = 44/50 (88.00%), Query Frame = 0

Query: 41 VNGN--DCYWFEEIIDENLKWSFALNSVLQKGTSEFQDIALLDTKCFGKV 89
          +NGN  DC WFEE+ID++LKWSFALN VL KGTSEFQDIALLDTK FGKV
Sbjct: 25 INGNLQDCCWFEEVIDDDLKWSFALNRVLHKGTSEFQDIALLDTKRFGKV 74

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023546524.19.2e-43100.00thermospermine synthase ACAULIS5-like [Cucurbita pepo subsp. pepo][more]
XP_022961616.12.1e-4298.85thermospermine synthase ACAULIS5 [Cucurbita moschata][more]
XP_022997196.13.5e-4298.85thermospermine synthase ACAULIS5-like [Cucurbita maxima][more]
XP_008445532.23.2e-3587.50PREDICTED: thermospermine synthase ACAULIS5 [Cucumis melo][more]
XP_022131411.12.4e-3081.61thermospermine synthase ACAULIS5 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G19530.13.1e-1676.60S-adenosyl-L-methionine-dependent methyltransferases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9S7X6|ACL5_ARATH5.5e-1576.60Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BCH1|A0A1S3BCH1_CUCME2.1e-3587.50thermospermine synthase ACAULIS5 OS=Cucumis melo OX=3656 GN=LOC103488520 PE=3 SV... [more]
tr|A0A0A0KIK6|A0A0A0KIK6_CUCSA2.8e-2471.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G362930 PE=3 SV=1[more]
tr|A0A2I4EZY8|A0A2I4EZY8_9ROSI2.4e-1555.68thermospermine synthase ACAULIS5 OS=Juglans regia OX=51240 GN=LOC108994283 PE=3 ... [more]
tr|V4UPQ2|V4UPQ2_9ROSI1.0e-1380.00Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10008847mg PE=3 ... [more]
tr|A0A067GE17|A0A067GE17_CITSI1.0e-1380.00Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g019550mg PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR030374PABS
IPR037163Spermidine_synt_N_sf
IPR035246Spermidine_synt_N
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09309-RACarg09309-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 47..88
e-value: 2.0E-8
score: 33.9
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3DG3DSA:2.30.140.10coord: 36..90
e-value: 2.9E-10
score: 42.4
NoneNo IPR availablePANTHERPTHR43317:SF2SUBFAMILY NOT NAMEDcoord: 24..89
NoneNo IPR availablePANTHERPTHR43317FAMILY NOT NAMEDcoord: 24..89
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 46..105
score: 13.227